projectin, twitchin, l(4)23, l(4)2, CT8086
Please see the JBrowse view of Dmel\bt for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.55
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.47
5.286 (compiled cDNA)
>20 (northern blot)
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bt using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
bt protein was immunolocalized to indirect flight muscle with a Lethocerus antibody. It localizes to flight muscles in the region containing C-filaments immediately adjacent to Z-discs.
bt protein is first detected by westerns blots at the mid-embryo stage and increases progressively until the end of the adult stage. The location of bt protein differs in nonfibrillar muscle from that in flight muscles. In the latter, it is located around the Z disc while in tubular muscle, it is located on the A band with a gap in the middle.
JBrowse - Visual display of RNA-Seq signals
View Dmel\bt in JBrowse4-0
4-1.4
Mapped in diplo-4 triploids.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
"Identified with: GM13171" was stated as revision. "Identified with: GM10074" was stated as revision. "Identified with: HL02324" was stated as revision. "Identified with: HL05966" was stated as revision.
The genomic organisation of the bt locus has been characterised.
The bt genomic region has been analysed.
Isolation and partial characterization of bt, includes identification of twitchin-like motifs.
An analysis of bt gene product, projectin, was performed to present evidence that projectin and twitchin may share functional protein kinase domains. As described for titin, bt is also demonstrated to be phosphorylated in vivo at Ser residues.
bt is a single copy gene containing a two motif amino acid sequence pattern that characterizes twitchin and titin. bt appears to encode projectin: a muscle protein thought to play a structural role in asynchronous flight muscle.
A protein component of connecting filaments of both asynchronous and synchronous muscles. In synchronous muscles projectin is found in the region of the thick filaments; in asynchronous muscles it is between the Z-band and thick filaments. The projectin of the two classes of muscle are similar, if not identical, by SDS electrophoresis and peptide mapping.
Source for merge of: bt CG10285
Annotations CG10285, CG1479 merged as CG32019 in release 3 of the genome annotation.
Hochman isolated 3 spontaneous alleles, 6 X ray induced alleles, 23 ethyl methanesulfonate induced alleles and 3 ICR170 induced alleles.
Source for identity of: bt CG1479