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Gene Dmel\Vha55

General Information
Symbol	Dmel\Vha55	Species	D. melanogaster
Name	Vacuolar H+-ATPase 55kD B subunit	Annotation symbol	CG17369
Feature type	protein_coding_gene	FlyBase ID	FBgn0005671
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	l(3)87Ca, SzA, l(3)j2E9
Genomic Location
Chromosome (arm)	3R	Recombination map	3-51.7
Cytogenetic map	87C2-87C3	Sequence location	3R:8,449,484..8,453,551 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vacuolar H+-ATPase 55kD B subunit is referred to in FlyBase by the symbol Dmel\Vha55 (CG17369, FBgn0005671). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: hydrogen-exporting ATPase activity, phosphorylative mechanism. It is reported to be involved in the biological process: proton transport. 25 alleles are reported. The phenotype of these alleles is annotated with: Malpighian tubule. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal; ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal; ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain; ATPase, V1 complex, subunit B; ATPase, alpha/beta subunit, nucleotide-binding domain, active site; V-type ATP synthase regulatory subunit B/beta. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1625131_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Vha55. Gene sequence location is 3R:8449484..8453551.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(3)87Ca Most extreme hemizygous phenotype is embryonic lethality, even though homozygous Df(3R)kar3J, which lacks the locus entirely, dies during the first larval instar (Ish-Horowicz, Holden, and Gehring, 1977, Cell 12: 643-52). First instar larvae have transparent Malpighian tubules; autonomous in transplants. Two alleles isolated on the basis of their dominant suppression of ectopic sex-comb teeth in Pc4/+ males (Kennison and Tamkun, 1988, Proc. Nat. Acad. Sci. USA 85: 8136-40).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Sequence features HFA16896 BKN21075 References Adamson et al., 2011, Genetics 189(2): 495--506
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87C2-87C3   Limits computationally determined from genome sequence between P{PZ}svp07842 and P{lacW}Vha55j2E9
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87C1-87C3   (determined by in situ hybridisation)   (Deak, 1999.12.22) 87C2-87C3   (determined by in situ hybridisation)   (Spradling et al., 1999) 87C1-87C3   (determined by in situ hybridisation)   (Szeged Stock Center, 1998-) 87C2-87C3     (Dow, 1999) 87C-87C     (Dow et al., 1997) 87C2-87C4   (determined by in situ hybridisation)   (Davies et al., 1996) 87C2-87C3     (BDGP Project Members, 1994-1999) 87C4-87C5   Determined by complementation analysis with deficiency and duplication chromosomes (details unspecified).   (Kennison and Tamkun, 1988)
Experimentally Determined Recombination Data
Location	3-51.7 (Kennison and Tamkun, 1988, )
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vha55 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vha55-RA FBtr0082671  NM_169475   2658   490 Vha55-RB FBtr0082670  NM_057560   2722   490 Vha55-RC FBtr0301661  NM_001170126   2048   490
Additional Transcript Data & Comments
Reported size (kB)	2.8, 2.6, 2.5, 2.0, 1.8 (longest cDNA) (Davies et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vha55-PA   FBpp0082140   54.5   490   5.05     NP_731726   AAF54836 Vha55-PB   FBpp0082139   54.5   490   5.05     NP_476908   AAF54837 Vha55-PC   FBpp0290875   54.5   490   5.05     NP_001163597   ACZ94894
Additional Polypeptide Data & Comments
Reported size (kDa)	490 (aa); 55 (kD) (Davies et al., 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (IPR000194) ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (IPR000793) ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal (IPR004100) ATPase, V1 complex, subunit B (IPR005723) ATPase, alpha/beta subunit, nucleotide-binding domain, active site (IPR020003) V-type ATP synthase regulatory subunit B/beta (IPR022879)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246500   AA264040   AA439281   AA540205   AA540369   AA540372   AA695147   AA695528   AA695642   AA698584   AA803132   AA804036   AA821029   AA821078   AA951166   AA979024   AE014297 AAF54836   AE014297 AAF54837   AC007668   AC007752   AE014297 ACZ94894   AI062743   AI062814   AI063104   AI109328   AI134990   AI135559   AI258765   AI261141   AI294620   AI294734   AI295417   AI295830   AI295836   AI295966   AI297083   AI297192   AI297525   AI456435   AI514090   AI530801   AI533849   AI546674   AK255236   AQ026310   AW941902   AY051623 AAK93047   BE662088   BE976783   BF486804   BF487276   BF487383   BF488302   BF489898   BF498226   BG633337   BG636047   BG636948   BG637066   BI162433   BI163333   BI163754   BI165037   BI169452   BI170544   BI171217   BI173458   BI214403   BI229026   BI231334   BI231443   BI236856   BI238200   BI238577   BI239055   BI240007   BI241642   BI241854   BI242266   BI353059   BI354209   BI354211   BI355988   BI358043   BI363061   BI363095   BI363493   BI363726   BI364257   BI364807   BI365280   BI365841   BI366023   BI368445   BI368552   BI369082   BI369789   BI371559   BI374243   BI374518   BI375859   BI482430   BI483281   BI484210   BI484332   BI485264   BI485558   BI485578   BI563577   BI564523   BI564611   BI565835   BI573484   BI573485   BI583027   BI584740   BI585512   BI587690   BI588415   BI588421   BI589325   BI607154   BI609533   BI609846   BI613969   BI617919   BI618030   BI618159   BI619225   BI621937   BI622988   BI623064   BI623143   BI624223   BI625942   BI626548   BI626687   BI626760   BI627113   BI627435   BI627537   BI632967   BI637093   BI637502   BI640328   BI640347   BP541527   BP547652   BT001302 AAN71057   CA803239   CA803483   CA806543   CK134764   CK661302   CO178814   CO190748   CO193432   CO193906   CO273341   CO277725   CO292649   CO294463   CO294798   CO296843   CO297558   CO302392   CO305115   CO305349   CO305468   CO310081   CO310299   CO311571   CO314991   CO321148   CO332232   CO343606   CZ486393   CZ486394   DQ507282   EC064196   EC064349   EC064493   EC065473   EC067799   EC069228   EC076238   EC076892   EC080559   EC085331   EC088635   EC088686   EC088813   EC194435   EC194436   EC201361   EC202047   EC206492   EC217359   EC221619   EC222434   EC224128   EC236566   EC237730   EC239558   EC240619   EC244384   EC245948   EC248831   EC260611   EC264383   EC266204   EF091933   EL875109   EL875110   EV577573   EV579245   EV588067   HQ383989   X67839 CAA48034
UniProtKB/Swiss-Prot	P31409
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
	Northern blots show Vha55 to be ubiquitously expressed, and reveal 4 bands of 2.8, 2.5, 2.0 and 1.8 kb. (Davies et al., 1996)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0005671    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0005671 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0005671 expression level Developmental Stage   Expression Level embryo 00-02hr    23075 embryo 02-04hr    5308 embryo 04-06hr    1619 embryo 06-08hr    3008 embryo 08-10hr    5292 embryo 10-12hr    6735 embryo 12-14hr    7534 embryo 14-16hr    19516 embryo 16-18hr    29144 embryo 18-20hr    40944 embryo 20-22hr    57828 embryo 22-24hr    58567 larva L1    82911 larva L2    54346 larva L3 12hr old    42587 larva L3 puffstage 1-2    24519 larva L3 puffstage 3-6    20242 larva L3 puffstage 7-9    15469 white prepupae new    11090 white prepupae 12hr    10944 white prepupae 24hr    6848 pupae 2d postWPP    8968 pupae 3d postWPP    12308 pupae 4d postWPP    30680 adult male 01day    28046 adult male 05day    24853 adult male 30day    26988 adult female 01day    39918 adult female 05day    27175 adult female 30day    25480 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (23075) embryo 02-04hr  (5308) embryo 04-06hr  (1619) embryo 06-08hr  (3008) embryo 08-10hr  (5292) embryo 10-12hr  (6735) embryo 12-14hr  (7534) embryo 14-16hr  (19516) embryo 16-18hr  (29144) embryo 18-20hr  (40944) embryo 20-22hr  (57828) embryo 22-24hr  (58567) larva L1  (82911) larva L2  (54346) larva L3 12hr old  (42587) larva L3 puffstage 1-2  (24519) larva L3 puffstage 3-6  (20242) larva L3 puffstage 7-9  (15469) white prepupae new  (11090) white prepupae 12hr  (10944) white prepupae 24hr  (6848) pupae 2d postWPP  (8968) pupae 3d postWPP  (12308) pupae 4d postWPP  (30680) adult male 01day  (28046) adult male 05day  (24853) adult male 30day  (26988) adult female 01day  (39918) adult female 05day  (27175) adult female 30day  (25480) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (23075) embryo 02-04hr  (5308) embryo 04-06hr  (1619) embryo 06-08hr  (3008) embryo 08-10hr  (5292) embryo 10-12hr  (6735) embryo 12-14hr  (7534) embryo 14-16hr  (19516) embryo 16-18hr  (29144) embryo 18-20hr  (40944) embryo 20-22hr  (57828) embryo 22-24hr  (58567) larva L1  (82911) larva L2  (54346) larva L3 12hr old  (42587) larva L3 puffstage 1-2  (24519) larva L3 puffstage 3-6  (20242) larva L3 puffstage 7-9  (15469) white prepupae new  (11090) white prepupae 12hr  (10944) white prepupae 24hr  (6848) pupae 2d postWPP  (8968) pupae 3d postWPP  (12308) pupae 4d postWPP  (30680) adult male 01day  (28046) adult male 05day  (24853) adult male 30day  (26988) adult female 01day  (39918) adult female 05day  (27175) adult female 30day  (25480) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (23075) embryo 02-04hr    5308 embryo 04-06hr    1619 embryo 06-08hr    3008 embryo 08-10hr    5292 embryo 10-12hr    6735 embryo 12-14hr    7534 embryo 14-16hr  (19516) embryo 16-18hr  (29144) embryo 18-20hr  (40944) embryo 20-22hr  (57828) embryo 22-24hr  (58567) larva L1  (82911) larva L2  (54346) larva L3 12hr old  (42587) larva L3 puffstage 1-2  (24519) larva L3 puffstage 3-6  (20242) larva L3 puffstage 7-9  (15469) white prepupae new  (11090) white prepupae 12hr  (10944) white prepupae 24hr    6848 pupae 2d postWPP    8968 pupae 3d postWPP  (12308) pupae 4d postWPP  (30680) adult male 01day  (28046) adult male 05day  (24853) adult male 30day  (26988) adult female 01day  (39918) adult female 05day  (27175) adult female 30day  (25480) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    23075 embryo 02-04hr    5308 embryo 04-06hr    1619 embryo 06-08hr    3008 embryo 08-10hr    5292 embryo 10-12hr    6735 embryo 12-14hr    7534 embryo 14-16hr    19516 embryo 16-18hr    29144 embryo 18-20hr    40944 embryo 20-22hr    57828 embryo 22-24hr    58567 larva L1    82911 larva L2    54346 larva L3 12hr old    42587 larva L3 puffstage 1-2    24519 larva L3 puffstage 3-6    20242 larva L3 puffstage 7-9    15469 white prepupae new    11090 white prepupae 12hr    10944 white prepupae 24hr    6848 pupae 2d postWPP    8968 pupae 3d postWPP    12308 pupae 4d postWPP    30680 adult male 01day    28046 adult male 05day    24853 adult male 30day    26988 adult female 01day    39918 adult female 05day    27175 adult female 30day    25480 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0005671 expression level Developmental Stage   Expression Level embryo 00-02hr    23075 embryo 02-04hr    5308 embryo 04-06hr    1619 embryo 06-08hr    3008 embryo 08-10hr    5292 embryo 10-12hr    6735 embryo 12-14hr    7534 embryo 14-16hr    19516 embryo 16-18hr    29144 embryo 18-20hr    40944 embryo 20-22hr    57828 embryo 22-24hr    58567 larva L1    82911 larva L2    54346 larva L3 12hr old    42587 larva L3 puffstage 1-2    24519 larva L3 puffstage 3-6    20242 larva L3 puffstage 7-9    15469 white prepupae new    11090 white prepupae 12hr    10944 white prepupae 24hr    6848 pupae 2d postWPP    8968 pupae 3d postWPP    12308 pupae 4d postWPP    30680 adult male 01day    28046 adult male 05day    24853 adult male 30day    26988 adult female 01day    39918 adult female 05day    27175 adult female 30day    25480 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (23075) embryo 02-04hr  (5308) embryo 04-06hr  (1619) embryo 06-08hr  (3008) embryo 08-10hr  (5292) embryo 10-12hr  (6735) embryo 12-14hr  (7534) embryo 14-16hr  (19516) embryo 16-18hr  (29144) embryo 18-20hr  (40944) embryo 20-22hr  (57828) embryo 22-24hr  (58567) larva L1  (82911) larva L2  (54346) larva L3 12hr old  (42587) larva L3 puffstage 1-2  (24519) larva L3 puffstage 3-6  (20242) larva L3 puffstage 7-9  (15469) white prepupae new  (11090) white prepupae 12hr  (10944) white prepupae 24hr  (6848) pupae 2d postWPP  (8968) pupae 3d postWPP  (12308) pupae 4d postWPP  (30680) adult male 01day  (28046) adult male 05day  (24853) adult male 30day  (26988) adult female 01day  (39918) adult female 05day  (27175) adult female 30day  (25480) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (23075) embryo 02-04hr  (5308) embryo 04-06hr  1619 embryo 06-08hr  (3008) embryo 08-10hr  (5292) embryo 10-12hr  (6735) embryo 12-14hr  (7534) embryo 14-16hr  (19516) embryo 16-18hr  (29144) embryo 18-20hr  (40944) embryo 20-22hr  (57828) embryo 22-24hr  (58567) larva L1  (82911) larva L2  (54346) larva L3 12hr old  (42587) larva L3 puffstage 1-2  (24519) larva L3 puffstage 3-6  (20242) larva L3 puffstage 7-9  (15469) white prepupae new  (11090) white prepupae 12hr  (10944) white prepupae 24hr  (6848) pupae 2d postWPP  (8968) pupae 3d postWPP  (12308) pupae 4d postWPP  (30680) adult male 01day  (28046) adult male 05day  (24853) adult male 30day  (26988) adult female 01day  (39918) adult female 05day  (27175) adult female 30day  (25480) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (23075) embryo 02-04hr    5308 embryo 04-06hr    1619 embryo 06-08hr    3008 embryo 08-10hr    5292 embryo 10-12hr    6735 embryo 12-14hr    7534 embryo 14-16hr  19516 embryo 16-18hr  (29144) embryo 18-20hr  (40944) embryo 20-22hr  (57828) embryo 22-24hr  (58567) larva L1  (82911) larva L2  (54346) larva L3 12hr old  (42587) larva L3 puffstage 1-2  (24519) larva L3 puffstage 3-6  20242 larva L3 puffstage 7-9  15469 white prepupae new  11090 white prepupae 12hr  10944 white prepupae 24hr    6848 pupae 2d postWPP    8968 pupae 3d postWPP  12308 pupae 4d postWPP  (30680) adult male 01day  (28046) adult male 05day  (24853) adult male 30day  (26988) adult female 01day  (39918) adult female 05day  (27175) adult female 30day  (25480) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    23075 embryo 02-04hr    5308 embryo 04-06hr    1619 embryo 06-08hr    3008 embryo 08-10hr    5292 embryo 10-12hr    6735 embryo 12-14hr    7534 embryo 14-16hr    19516 embryo 16-18hr    29144 embryo 18-20hr    40944 embryo 20-22hr    57828 embryo 22-24hr    58567 larva L1    82911 larva L2    54346 larva L3 12hr old    42587 larva L3 puffstage 1-2    24519 larva L3 puffstage 3-6    20242 larva L3 puffstage 7-9    15469 white prepupae new    11090 white prepupae 12hr    10944 white prepupae 24hr    6848 pupae 2d postWPP    8968 pupae 3d postWPP    12308 pupae 4d postWPP    30680 adult male 01day    28046 adult male 05day    24853 adult male 30day    26988 adult female 01day    39918 adult female 05day    27175 adult female 30day    25480 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0005671    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0005671 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0005671 expression level Tissue   Expression Level Larval Central Nervous System    1120.475 Larval Midgut    3636.9 Larval Hindgut    3827.3 Larval Malpighian Tubules    2817.4 Larval Fat Body    829.6 Larval Salivary Gland    2265.9 Larval Trachea    1285.75 Larval Carcass    1772.625 Adult Head    1395.4 Adult Eye    911.05 Adult Brain    1856 Adult Thoracic-Abdominal Ganglion    1967.4 Adult Crop    1075.5 Adult Midgut    2366.9 Adult Hindgut    3011 Adult Malpighian Tubules    4016.2 Adult Fat Body    706.8 Adult Salivary Gland    6275.2 Adult Heart    579.925 Adult VirginFemale Spermatheca    982.9 Adult InseminatedFemale Spermatheca    1009 Adult Ovary    1146.8 Adult Testis    227.7 Adult Male Accessory Gland    608.2 Adult Carcass    990.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1120.475) Larval Midgut  (3636.9) Larval Hindgut  (3827.3) Larval Malpighian Tubules  (2817.4) Larval Fat Body  (829.6) Larval Salivary Gland  (2265.9) Larval Trachea  (1285.75) Larval Carcass  (1772.625) Adult Head  (1395.4) Adult Eye  (911.05) Adult Brain  (1856) Adult Thoracic-Abdominal Ganglion  (1967.4) Adult Crop  (1075.5) Adult Midgut  (2366.9) Adult Hindgut  (3011) Adult Malpighian Tubules  (4016.2) Adult Fat Body  (706.8) Adult Salivary Gland  (6275.2) Adult Heart  (579.925) Adult VirginFemale Spermatheca  (982.9) Adult InseminatedFemale Spermatheca  (1009) Adult Ovary  (1146.8) Adult Testis    227.7 Adult Male Accessory Gland  (608.2) Adult Carcass  (990.8) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1120.475) Larval Midgut  (3636.9) Larval Hindgut  (3827.3) Larval Malpighian Tubules  (2817.4) Larval Fat Body    829.6 Larval Salivary Gland  (2265.9) Larval Trachea  (1285.75) Larval Carcass  (1772.625) Adult Head  (1395.4) Adult Eye    911.05 Adult Brain  (1856) Adult Thoracic-Abdominal Ganglion  (1967.4) Adult Crop  1075.5 Adult Midgut  (2366.9) Adult Hindgut  (3011) Adult Malpighian Tubules  (4016.2) Adult Fat Body    706.8 Adult Salivary Gland  (6275.2) Adult Heart    579.925 Adult VirginFemale Spermatheca    982.9 Adult InseminatedFemale Spermatheca  1009 Adult Ovary  (1146.8) Adult Testis    227.7 Adult Male Accessory Gland    608.2 Adult Carcass    990.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1120.475 Larval Midgut    3636.9 Larval Hindgut    3827.3 Larval Malpighian Tubules    2817.4 Larval Fat Body    829.6 Larval Salivary Gland    2265.9 Larval Trachea    1285.75 Larval Carcass    1772.625 Adult Head    1395.4 Adult Eye    911.05 Adult Brain    1856 Adult Thoracic-Abdominal Ganglion    1967.4 Adult Crop    1075.5 Adult Midgut    2366.9 Adult Hindgut    3011 Adult Malpighian Tubules    4016.2 Adult Fat Body    706.8 Adult Salivary Gland    6275.2 Adult Heart    579.925 Adult VirginFemale Spermatheca    982.9 Adult InseminatedFemale Spermatheca    1009 Adult Ovary    1146.8 Adult Testis    227.7 Adult Male Accessory Gland    608.2 Adult Carcass    990.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0005671 expression level Tissue   Expression Level Larval Central Nervous System    1120.475 Larval Midgut    3636.9 Larval Hindgut    3827.3 Larval Malpighian Tubules    2817.4 Larval Fat Body    829.6 Larval Salivary Gland    2265.9 Larval Trachea    1285.75 Larval Carcass    1772.625 Adult Head    1395.4 Adult Eye    911.05 Adult Brain    1856 Adult Thoracic-Abdominal Ganglion    1967.4 Adult Crop    1075.5 Adult Midgut    2366.9 Adult Hindgut    3011 Adult Malpighian Tubules    4016.2 Adult Fat Body    706.8 Adult Salivary Gland    6275.2 Adult Heart    579.925 Adult VirginFemale Spermatheca    982.9 Adult InseminatedFemale Spermatheca    1009 Adult Ovary    1146.8 Adult Testis    227.7 Adult Male Accessory Gland    608.2 Adult Carcass    990.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1120.475) Larval Midgut  (3636.9) Larval Hindgut  (3827.3) Larval Malpighian Tubules  (2817.4) Larval Fat Body  (829.6) Larval Salivary Gland  (2265.9) Larval Trachea  (1285.75) Larval Carcass  (1772.625) Adult Head  (1395.4) Adult Eye  (911.05) Adult Brain  (1856) Adult Thoracic-Abdominal Ganglion  (1967.4) Adult Crop  (1075.5) Adult Midgut  (2366.9) Adult Hindgut  (3011) Adult Malpighian Tubules  (4016.2) Adult Fat Body  706.8 Adult Salivary Gland  (6275.2) Adult Heart  579.925 Adult VirginFemale Spermatheca  (982.9) Adult InseminatedFemale Spermatheca  (1009) Adult Ovary  (1146.8) Adult Testis    227.7 Adult Male Accessory Gland  608.2 Adult Carcass  (990.8) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1120.475 Larval Midgut  (3636.9) Larval Hindgut  (3827.3) Larval Malpighian Tubules  (2817.4) Larval Fat Body    829.6 Larval Salivary Gland  (2265.9) Larval Trachea  1285.75 Larval Carcass  (1772.625) Adult Head  1395.4 Adult Eye    911.05 Adult Brain  (1856) Adult Thoracic-Abdominal Ganglion  (1967.4) Adult Crop  1075.5 Adult Midgut  (2366.9) Adult Hindgut  (3011) Adult Malpighian Tubules  (4016.2) Adult Fat Body    706.8 Adult Salivary Gland  (6275.2) Adult Heart    579.925 Adult VirginFemale Spermatheca    982.9 Adult InseminatedFemale Spermatheca  1009 Adult Ovary  1146.8 Adult Testis    227.7 Adult Male Accessory Gland    608.2 Adult Carcass    990.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1120.475 Larval Midgut    3636.9 Larval Hindgut    3827.3 Larval Malpighian Tubules    2817.4 Larval Fat Body    829.6 Larval Salivary Gland    2265.9 Larval Trachea    1285.75 Larval Carcass    1772.625 Adult Head    1395.4 Adult Eye    911.05 Adult Brain    1856 Adult Thoracic-Abdominal Ganglion    1967.4 Adult Crop    1075.5 Adult Midgut    2366.9 Adult Hindgut    3011 Adult Malpighian Tubules    4016.2 Adult Fat Body    706.8 Adult Salivary Gland    6275.2 Adult Heart    579.925 Adult VirginFemale Spermatheca    982.9 Adult InseminatedFemale Spermatheca    1009 Adult Ovary    1146.8 Adult Testis    227.7 Adult Male Accessory Gland    608.2 Adult Carcass    990.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1395.4   NA  Eye    911.05   NA  Brain    1856   1120.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1967.4   NA  Crop    1075.5   3636.9  Midgut    2366.9   3827.3  Hindgut    3011   2817.4  Malpighian Tubules    4016.2   829.6  Fat Body    706.8   2265.9  Salivary Gland    6275.2   NA  Heart    579.925   1285.75  Trachea    NA   NA  VirginFemale Spermatheca    982.9   NA  InseminatedFemale Spermatheca    1009   NA  Ovary    1146.8   NA  Testis    227.7   NA  Male Accessory Gland    608.2   1772.625  Carcass    990.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_20
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005671 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17369-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Vha55 Stages(s) 4-6 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Vha55CA07634 lethal | embryonic stage Vha551 Vha557a1 Vha559 Vha5512 Vha55j2E9 lethal | embryonic stage | recessive Vha557 Vha55j2E9 lethal | first instar larval stage Vha5514 Vha55S120813b lethal | larval stage | recessive Vha552 Vha55S120813b lethal | pupal stage, with Scer\GAL4pnr-MD237 Vha55GD9363 lethal | recessive Vha551 Vha553 Vha554 Vha555 Vha556 Vha558 Vha559 Vha5510 Vha5511 Vha5512 Vha5513 Vha5515 Vha5516 Vha55j2E9 Vha55S120813b semi-lethal Vha553 Vha5510 Other Phenotypes Allele decreased cell number | larval stage | somatic clone Vha55j2E9 size defective | larval stage Vha55j2E9 Phenotype manifest in Allele eye disc | somatic clone Vha55j2E9 Malpighian tubule Vha551 Vha5512 Vha5514 Vha552 Vha554 Vha555 Vha557 Vha55S120813b Vha55j2E9 Vha557a1 multivesicular body | larval stage | somatic clone Vha55j2E9
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of Vha55 Class Mutagen Stocks Known lesion Vha5514 2 Yes Vha55j2E9 amorphic allele - genetic evidence P-element activity 1 Yes Vha5512 hypomorphic allele - genetic evidence ethyl methanesulfonate 1 -- Vha5516 ethyl methanesulfonate 1 -- Vha55f03804 piggyBac transposase 1 -- Vha55MI01500 1 -- Vha5510 ethyl methanesulfonate 0 -- Vha5511 ethyl methanesulfonate 0 -- Vha5513 ethyl methanesulfonate 0 -- Vha5515 gamma ray 0 -- Vha551 ethyl methanesulfonate 0 -- Vha552 ethyl methanesulfonate 0 -- Vha553 ethyl methanesulfonate 0 -- Vha554 ethyl methanesulfonate 0 -- Vha555 ethyl methanesulfonate 0 -- Vha556 ethyl methanesulfonate 0 -- Vha557 ethyl methanesulfonate 0 -- Vha557a1 ethyl methanesulfonate 0 -- Vha558 ethyl methanesulfonate 0 -- Vha559 ethyl methanesulfonate 0 -- Vha55CA07634 P-element activity 0 Yes Vha55CPTI002645 piggyBac transposase 0 Yes Vha55CPTI100063 piggyBac transposase 0 Yes Vha55S120813b Delta2-3 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vha55 Class Mutagen Stocks Known lesion Vha55GD9363 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)615 (McCarron et al., 1994) Df(3R)2515 (McCarron et al., 1994) Df(3R)2519 (McCarron et al., 1994) Df(3R)2521 (McCarron et al., 1994) Df(3R)2523 (McCarron et al., 1994) Df(3R)2525 (McCarron et al., 1994) Df(3R)2526 (McCarron et al., 1994) Df(3R)2527 (McCarron et al., 1994) Df(3R)BSC486 (Christensen et al., 2008.4.15) Df(3R)Exel7317 (Ryder, 2004.4.26) Df(3R)kar3J (Gausz et al., 1979, Davies et al., 1996) Df(3R)kar3l (Gausz et al., 1979) Df(3R)kar3Q (Gausz et al., 1979) Df(3R)kar-D1 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(3R)kar-H5 (McCarron et al., 1994) Df(3R)kar-Sz12 (Gausz et al., 1979) Df(3R)kar-Sz29 (Gausz et al., 1979, Davies et al., 1996) Df(3R)T-10 (Gausz et al., 1979) Df(3R)T-32 (Szeged Stock Center, 1998-, Gausz et al., 1979)
Not disrupted in	Df(3R)2520 (McCarron et al., 1994) Df(3R)2522 (McCarron et al., 1994) Df(3R)2524 (McCarron et al., 1994) Df(3R)2528 (McCarron et al., 1994) Df(3R)kar-Sz11 (Gausz et al., 1979, Davies et al., 1996) Df(3R)kar-Sz21 (Gausz et al., 1979) Df(3R)kar-Sz27 (Gausz et al., 1979) Df(3R)kar-Sz31 (Gausz et al., 1979, Davies et al., 1996) Df(3R)kar-Sz37 (Gausz et al., 1979, Davies et al., 1996) Df(3R)M-Kx1 (Szeged Stock Center, 1998-)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9363} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Vha55j2E9 No P{lacW}Vha55S120813b No miscellaneous insertions Mi{MIC}Vha55MI01500 NA P{PTT-GA}Vha55CA07634 NA PBac{544.SVS-1}Vha55CPTI100063 NA PBac{769.FSVS-1}Vha55CPTI002645 NA PBac{WH}Vha55f03804 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
early endosome inferred from direct assay (Phillips and Thomas, 2006) plasma membrane inferred from direct assay (Khanna et al., 2010)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000194, InterPro:IPR020003 (FlyBase Curators et al., 2004-) hydrogen-exporting ATPase activity, phosphorylative mechanism inferred from sequence or structural similarity with EMBL:Y12634 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces VMA2 (Dow, 1999) non-traceable author statement (Swiss-Prot Project Members, 1993.7.1) hydrogen ion transporting ATP synthase activity, rotational mechanism inferred from electronic annotation with InterPro:IPR004100 (FlyBase Curators et al., 2004-) proton-transporting ATPase activity, rotational mechanism inferred from electronic annotation with InterPro:IPR004100 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
ATP hydrolysis coupled proton transport inferred from electronic annotation with InterPro:IPR000793, InterPro:IPR005723 (FlyBase Curators et al., 2004-) ATP metabolic process inferred from electronic annotation with InterPro:IPR004100 (FlyBase Curators et al., 2004-) proton transport non-traceable author statement (Swiss-Prot Project Members, 1993.7.1)
Cellular Component
CV term Evidence References
vacuolar proton-transporting V-type ATPase, V1 domain inferred from sequence or structural similarity with EMBL:Y12634 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces VMA2 (Dow, 1999) non-traceable author statement (Swiss-Prot Project Members, 1993.7.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vha55 allele Gene References Vha557 corto (Lopez et al., 2001) Vha5514 corto (Lopez et al., 2001) Vha5515 Pc (Kennison and Tamkun, 1988) Vha5516 Pc (Kennison and Tamkun, 1988) Vha55j2E9 Kr (Carrera et al., 1998, Abrell et al., 2000) Pc (Davies et al., 1996)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66645 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005671 DroID - A comprehensive database of gene and protein interactions. • FBgn0005671 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41550
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23001 Dere\GG17111 Dgri\GH18893 Dmoj\GI24752 Dper\GL13570 Dpse\GA14484 Dsec\GM25995 Dsim\GD20555 Dvir\GJ14577 Dwil\GK13566 Dyak\GE24501
Curated drosophilid orthologs	Dpse\GA14484
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005798-PA Aedes aegypti (mosquito) XP_624112.1 Apis mellifera (honey bee) XP_001951369.1 Acyrthosiphon pisum (pea aphid) At1g76030.1 Arabidopsis thaliana (wall cress) 19451 Batrachochytrium dendrobatidis (chytrid fungus) 91054 Branchiostoma floridae (lancelet) BGIBMGA002241-PA Bombyx mori (silk worm) ENSBTAP00000024812 Bos taurus (bovine) CAL0004009 Candida albicans (a form of yeast) CN01519 Caenorhabditis brenneri (small nematode) CBP18033 Caenorhabditis briggsae (nematode) CE04424 Caenorhabditis elegans (worm) ENSCAFP00000014848 Canis familiaris (dog) CAGL0C00649g Candida glabrata (haploid yeast) Chro.80195 Cryptosporidium hominis (obligate parasite) ENSCINP00000013764 Ciona intestinalis (sea squirt) JA04275 Caenorhabditis japonica (nematode) CMP123C Cyanidioschyzon merolae (red alga) cn08114 Cryptococcus neoformans (yeast-like fungus) cgd8_1670 Cryptosporidium parvum (protozoan parasite) CPIJ003418 Culex pipens (mosquito) ENSCPOP00000001580 Cavia porcellus (guinea pig) 126382 Chlamidomonas reinhardtii (green alga) RP30160 Caenorhabditis remanei (nematode) ENSCSAVP00000012828 Ciona savignyi (sedentary tunicate) 180714 Capitella spl (polychaete worm) DDB0185207 Dictyostelium discoideum (amoeba) DEHA2E21846g Debaryomyces Hansenii (yeast) 306451 Daphnia pulex (daphnia) ENSDARP00000063805 Danio rerio (zebra fish) ENSECAP00000014612 Equus caballus (horse) XP_656034 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000019332 Gymnopilus aculeatus (mushroom) ENSGALP00000040078 Gallus gallus (chicken) GL50803_12216 Giardia lamblia (flagellated protozoan) 186621 Hypsipyla robusta (cedar shoot borer) ENSP00000276390 Homo sapiens (human) ISCW022132-PA Ixodes scapularis (deer tick) KLLA0D09152g Kluyveromyces lactis (yeast) 119755 Lottia gigantea (owl limpet) LmjF28.2430 Leishmania major (virus) 38294 Monocosta brevicollis (choanoflagellate) ENSMODP00000012295 Monodelphis domestica (gray opossum) ENSMMUP00000026300 Macaca mulatta (rhesus monkey) ENSMUSP00000006435 Mus musculus (house mouse) 99968 Nematostella vectensis (starlet anemone) XP_001601249.1 Nasonia vitripennis (wasp) ENSOANP00000016761 Ornithorynchus anatinus (platypus) ENSORLP00000001501 Oryzias latipes (japanese killifish) LOC_Os06g37180.1 Oryza sativa (asian rice) 19782 Ostreococcus tauri (green alga) PFD0305c Plasmodium falciparum (protozoal parasite) PHUM005260-PA Pediculus humanus (human body louse) PP05611 Pristionchus pacificus (nematode) 106587 Physcomitrella patens (moss) 214369 Physcomitrella patens (moss) ENSPPYP00000013735 Pongo pygmaeus (orangutan) 72303 Phytophthora ramorum (sudden oak death pathogen) 108790 Phytophthora sojae (root rot pathogen) 821076 Populus trichocarpa (black cottonwood) ENSPTRP00000034316 Pan troglodytes (chimpanzee) PVX_000935 Plasmodium vivax (protozoal parasite) ENSRNOP00000015931 Rattus norvegicus (norway rat) 5011053 Sorghum bicolor (sorghum) YBR127C Saccharomyces cerevisiae (budding yeast) Smp_029390 Schistosoma mansoni (trematode parasite) SPAC637.05c Schizosaccharomyces pombe (fission yeast) XP_001176087.1 Strongylocentrotus purpuratus (purple sea urchin) 32492 Trichoplax adhaerens (metazoa) TA08450 Theileria annulata (apicomplexan parasite) XP_967844.1 Tribolium castaneum (red flour beetle) Tc00.1047053506025.50 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053511209.10 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000008539 Tetraodon nigroviridis (green spotted pufferfish) TP04_0441 Theileria parva (parasitic protozoan) 40522 Thalassiosira pseudonana (marine diatom) ENSTRUP00000047741 Takifugu rubripes (pufferfish Takifugu) 3684.m00040 Tetrahymena thermophila (ciliate protozoa) 91362.m00072 Trichomonas vaginalis (parasitic flagellated protozoan) 95937.m00092 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000035945 Xenopus tropicalis (frog) YALI0E27346g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12615 LH18180 ISCW022132 FBgn0241615 FBgn0215574 FBgn0201784 FBgn0192024 FBgn0180851 JGI_V11_306451 FBgn0074512 FBgn0151175 FBgn0147474 FBgn0126360 FBgn0109339 FBgn0099995 CPIJ014699 CPIJ003418 BGIBMGA002241 ACYPI010077 GB19171 AGAP002884 AAEL005798 PB22514 PHUM253140 GLEAN_12133
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	1617 Vha5514/MRS 9445 Vha5516/TM6C, Sb1 9446 Vha5512/TM3, Sb1 Ser1 35064 y1 w*; Mi{MIC}Vha55MI01500 12128 y1 w*; P{lacW}Vha55j2E9/TM3, Sb1
Harvard	- PBac{WH}Vha55f03804
Kyoto	101701 Vha5514 / MKRS, kar1 ry2 Sb1
VDRC	v46554 w1118 P{GD9363}v46554 v46553 w1118; P{GD9363}v46553/TM3
Genomic Clones ( 2 )
	BACR02G11 BACR07J06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 201 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT12604 GH27148
Other clones	DPiM_GH27148
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT22026 AT22165 AT23540 AT26081 GH02057 GH02157 GH02573 GH08478 GH12592 GH13363 GH24684 GM02316 GM02970 GM03168 GM05444 GM09610 GM10070 GM12608 GM22058 GM26849 GM26852 LD05423 LD07409 LD19613 LD19835 LD19843 LD33267 LD36456 LP02038 LP05261 LP08035 LP08175 LP09036 LP09581 LP09589 LP09744 LP11275 LP11441 LP11841 LP20692 RE01753 RE02711 RE03247 RE04839 RE10631 RE12047 RE12935 RE16379 RE20191 RE20785 RE21913 RE26722 RE32980 RE34594 RE35067 RE35445 RE36647 RE37063 RE38760 RE39042 RE39554 RE44709 RE47778 RE47825 RE48316 RE48655 RE49330 RE49991 RE51230 RE51455 RE54416 RE54542 RE55165 RE56085 RE58474 RE61785 RE62131 RE63754 RE65125 RE66153 RE67272 RE67419 RE68564 RE68914 RE68939 RH14328 RH14683 RH21459 RH24010 RH25113 RH28538 RH29623 RH29634 RH31736 RH34236 RH43240 RH48396 RH48546 RH48737 RH53727 RH55041 RH55133 RH55232 RH61825 RH61927 RH63662 RH63746 RH66454 RH67525 RH67693 RH67812 RH68323 RH68751 RH68881 RH74158 SD01523 SD05820 SD09887 SD13630 SD14779 SD14918 SD19009 SD19540 SD23154 SD23175 SD26877
Other clones	15954 19703 44201 53185 55932 72083 74981 79500 81719 83660 97956 98392 102111 118234 118972 119529 122893 123410 125210 145993 194383 214287.33 275187 571175 571177 AT21335 bs20f11 bs55g08 CK01.7C.D11 CK01.13B.H6 CK01.17A.D1 CK01.32D.C10 CK01.39A.C8 CK01.43C.H5 Dmel_dig_010612_0598_1472 Dmel_dig_019841_0661_3437 Dmel_neb_003244_0120_3740 EC35777 EC36977 EK049515 EK057631 EK110608 EK126123 EK170060 EK184049 EK200662 EK207013 EK207709 EK215257 EK220419 EK221046 EK225341 EK238659 EK247456 EK248261 EK248724 EK290410 EK302845 EP17346 ESG011b.E7_A09 ESG011b.E21_A09 ESG013d.E21_E09 FGC002F04 FGH119C04 HL04973 IP15156 LD13804 LD31489 RE50539 RE74748 RH09111 RH50213
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0005671 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41550
Antibody Information
	polyclonal (Pyza et al., 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against Vha55 that is transfected into S2 treated with Listeria monocytogenes reveals Vha55 to be involved in Listeria monocytogenes entry and vacuolar escape. (Cheng et al., 2005) Sections of the Scr regulatory region may be important for regulation of Scr by Polycomb- and trithorax-group genes. (Gindhart and Kaufman, 1995) Mutations of Vha55 interact with Dfd to reduce the viability of the Dfd3/Dfd13 combination. (Harding et al., 1995) Vha55 is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Alleles of Pc, Pcl, Scm, Dll, brm, kto, Scr and trx show clear dominant enhancement or suppression of AntpScx, whereas alleles of vtd, Vha55, Su(Pc)37D, urd, mor, skd and osa do not. (Kennison and Tamkun, 1988) Most extreme hemizygous phenotype is embryonic lethality, even though homozygous Df(3R)kar3J, which lacks the locus entirely, dies during the first larval instar (Ish-Horowicz, Holden and Gehring, 1977). First instar larvae have transparent Malpighian tubules; autonomous in transplants. Two alleles isolated on the basis of their dominant suppression of ectopic sex-comb teeth in Pc4/+ males (Kennison and Tamkun, 1988).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057560 NM_169475 NM_001170126 RefSeq (Proteins) • NP_476908 NP_731726 NP_001163597 DDBJ / EMBL / GenBank • AA695528 AAF54836 AAF54837 AAK93047 AAN71057 AC007668 AC007752 ACZ94894 AI456435 AI514090 AQ026310 AW941902 AY051623 BE662088 BF498226 BT001302 CAA48034 CZ486393 CZ486394 X67839 Entrez Gene - A searchable database of RefSeq genes. • 41550 UniProtKB/Swiss-Prot • P31409
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (IPR000194) ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (IPR000793) ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal (IPR004100) ATPase, V1 complex, subunit B (IPR005723) ATPase, alpha/beta subunit, nucleotide-binding domain, active site (IPR020003) V-type ATP synthase regulatory subunit B/beta (IPR022879)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66645 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005671 DroID - A comprehensive database of gene and protein interactions. • FBgn0005671 DRSC - Results from RNAi screens. • FBgn0005671 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005671 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17369-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0005671 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41550 InParanoid A subset of ortholog calls from InParanoid. • AAEL005798-PA Aedes aegypti (mosquito) XP_624112.1 Apis mellifera (honey bee) XP_001951369.1 Acyrthosiphon pisum (pea aphid) At1g76030.1 Arabidopsis thaliana (wall cress) 19451 Batrachochytrium dendrobatidis (chytrid fungus) 91054 Branchiostoma floridae (lancelet) BGIBMGA002241-PA Bombyx mori (silk worm) ENSBTAP00000024812 Bos taurus (bovine) CAL0004009 Candida albicans (a form of yeast) CN01519 Caenorhabditis brenneri (small nematode) CBP18033 Caenorhabditis briggsae (nematode) CE04424 Caenorhabditis elegans (worm) ENSCAFP00000014848 Canis familiaris (dog) CAGL0C00649g Candida glabrata (haploid yeast) Chro.80195 Cryptosporidium hominis (obligate parasite) ENSCINP00000013764 Ciona intestinalis (sea squirt) JA04275 Caenorhabditis japonica (nematode) CMP123C Cyanidioschyzon merolae (red alga) cn08114 Cryptococcus neoformans (yeast-like fungus) cgd8_1670 Cryptosporidium parvum (protozoan parasite) CPIJ003418 Culex pipens (mosquito) ENSCPOP00000001580 Cavia porcellus (guinea pig) 126382 Chlamidomonas reinhardtii (green alga) RP30160 Caenorhabditis remanei (nematode) ENSCSAVP00000012828 Ciona savignyi (sedentary tunicate) 180714 Capitella spl (polychaete worm) DDB0185207 Dictyostelium discoideum (amoeba) DEHA2E21846g Debaryomyces Hansenii (yeast) 306451 Daphnia pulex (daphnia) ENSDARP00000063805 Danio rerio (zebra fish) ENSECAP00000014612 Equus caballus (horse) XP_656034 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000019332 Gymnopilus aculeatus (mushroom) ENSGALP00000040078 Gallus gallus (chicken) GL50803_12216 Giardia lamblia (flagellated protozoan) 186621 Hypsipyla robusta (cedar shoot borer) ENSP00000276390 Homo sapiens (human) ISCW022132-PA Ixodes scapularis (deer tick) KLLA0D09152g Kluyveromyces lactis (yeast) 119755 Lottia gigantea (owl limpet) LmjF28.2430 Leishmania major (virus) 38294 Monocosta brevicollis (choanoflagellate) ENSMODP00000012295 Monodelphis domestica (gray opossum) ENSMMUP00000026300 Macaca mulatta (rhesus monkey) ENSMUSP00000006435 Mus musculus (house mouse) 99968 Nematostella vectensis (starlet anemone) XP_001601249.1 Nasonia vitripennis (wasp) ENSOANP00000016761 Ornithorynchus anatinus (platypus) ENSORLP00000001501 Oryzias latipes (japanese killifish) LOC_Os06g37180.1 Oryza sativa (asian rice) 19782 Ostreococcus tauri (green alga) PFD0305c Plasmodium falciparum (protozoal parasite) PHUM005260-PA Pediculus humanus (human body louse) PP05611 Pristionchus pacificus (nematode) 106587 Physcomitrella patens (moss) 214369 Physcomitrella patens (moss) ENSPPYP00000013735 Pongo pygmaeus (orangutan) 72303 Phytophthora ramorum (sudden oak death pathogen) 108790 Phytophthora sojae (root rot pathogen) 821076 Populus trichocarpa (black cottonwood) ENSPTRP00000034316 Pan troglodytes (chimpanzee) PVX_000935 Plasmodium vivax (protozoal parasite) ENSRNOP00000015931 Rattus norvegicus (norway rat) 5011053 Sorghum bicolor (sorghum) YBR127C Saccharomyces cerevisiae (budding yeast) Smp_029390 Schistosoma mansoni (trematode parasite) SPAC637.05c Schizosaccharomyces pombe (fission yeast) XP_001176087.1 Strongylocentrotus purpuratus (purple sea urchin) 32492 Trichoplax adhaerens (metazoa) TA08450 Theileria annulata (apicomplexan parasite) XP_967844.1 Tribolium castaneum (red flour beetle) Tc00.1047053506025.50 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053511209.10 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000008539 Tetraodon nigroviridis (green spotted pufferfish) TP04_0441 Theileria parva (parasitic protozoan) 40522 Thalassiosira pseudonana (marine diatom) ENSTRUP00000047741 Takifugu rubripes (pufferfish Takifugu) 3684.m00040 Tetrahymena thermophila (ciliate protozoa) 91362.m00072 Trichomonas vaginalis (parasitic flagellated protozoan) 95937.m00092 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000035945 Xenopus tropicalis (frog) YALI0E27346g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41550 modMine - Data generated by the modENCODE project. • FBgn0005671 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12615 LH18180 ISCW022132 FBgn0241615 FBgn0215574 FBgn0201784 FBgn0192024 FBgn0180851 JGI_V11_306451 FBgn0074512 FBgn0151175 FBgn0147474 FBgn0126360 FBgn0109339 FBgn0099995 CPIJ014699 CPIJ003418 BGIBMGA002241 ACYPI010077 GB19171 AGAP002884 AAEL005798 PB22514 PHUM253140 GLEAN_12133
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	1208/13 (Deak et al., 1997) ATP6V1B1 (Adamson et al., 2011) CG17369 (Vierstraete et al., 2003, Wang et al., 2004, Allan et al., 2005, Khanna et al., 2010, Cammarato et al., 2011) l(3)87Ca (Vazquez et al., 1999, Maixner et al., 1998, Deak et al., 1997, ) l(3)j2E9 (Abrell et al., 2000, Carrera et al., 1998, ) l(3)SzA (Gausz et al., 1979, ) SzA (Dow et al., 2003, Dow, 1999) VAT-2 (Mushegian et al., 1998) V-ATPase (Dow and Davies, 2000) vha55 (Cheng et al., 2005, Dow et al., 2003, Abrell et al., 2000, Dow, 1999, Deak et al., 1997, Sozen et al., 1997, Dow et al., 1997, Dow et al., 1997, Davies et al., 1996, Stark et al., 2005, Pyza et al., 2004, Wang et al., 2004, Allan et al., 2005, Du et al., 2006, Adamson et al., 2011) Vha55 (Pilot et al., 2006, Stuart et al., 2007, Buszczak et al., 2007, Christensen et al., 2008.4.15, Dorus et al., 2006, Xing et al., 2007, Vázquez et al., 2008, Yan et al., 2009, Gim et al., 2001, Hermle et al., 2010, Vaccari et al., 2010, Domanitskaya et al., 2007, Seong et al., 2011)
Name Synonym	B subunit (Dow et al., 1997) B subunit of V-ATPase (Vaccari et al., 2010) V1 B subunit (Yan et al., 2009) vacuolar ATPase B subunit (Dow, 1992.7.24) vacuolar ATP synthase subunit B (Domanitskaya et al., 2007) Vacuolar H+-ATPase 55-kDa B subunit (Gim et al., 2001) Vacuolar H+-ATPase 55kD B subunit (Xing et al., 2007, Khanna et al., 2010, Cammarato et al., 2011) V-ATPase B-subunit (Pyza et al., 2004) V-ATPase subunit B (Phillips and Thomas, 2006)
Secondary FlyBase IDs
	FBgn0002345 FBgn0011789
References ( 80 )
Generate a list of	All references relating to this gene ( 80 )
List References by type	Research paper  ( 43 ) Supplementary material  ( 7 ) Review  ( 3 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 5 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 5 ) Protein sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 4 )
	Adamson et al., 2011, Genetics 189(2): 495--506 A Drosophila model for genetic analysis of influenza viral/host interactions. [FBrf0216397] Seong et al., 2011, Biogerontology 12(2): 93--107 Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity. [FBrf0213307] Hermle et al., 2010, Curr. Biol. 20(14): 1269--1276 Regulation of Frizzled-Dependent Planar Polarity Signaling by a V-ATPase Subunit. [FBrf0211335] Vaccari et al., 2010, Development 137(11): 1825--1832 The vacuolar ATPase is required for physiological as well as pathological activation of the Notch receptor. [FBrf0210791] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...