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Gene Dmel\spi

General Information
Symbol	Dmel\spi	Species	D. melanogaster
Name	spitz	Annotation symbol	CG10334
Feature type	protein_coding_gene	FlyBase ID	FBgn0005672
Gene Model Status	Current	Stock availability	25 publicly available
Also Known As	Spitz
Genomic Location
Chromosome (arm)	2L	Recombination map	2-54
Cytogenetic map	37F2-37F2	Sequence location	2L:19,567,900..19,577,315 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spitz is referred to in FlyBase by the symbol Dmel\spi (CG10334, FBgn0005672). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: gurken receptor binding; epidermal growth factor receptor binding. There is experimental evidence for 14 unique biological process terms, many of which group under: single-organism developmental process; system development; cellular process; biological regulation; multicellular organismal development; cell fate commitment; response to organic substance; positive regulation of cell proliferation; localization; stem cell differentiation; stomatogastric nervous system development; post-embryonic organ morphogenesis; open tracheal system development; system process. 87 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; external compound sense organ; peripheral nervous system; ISN innervated muscle of A1-7; thoracic segment; adult segment; non-connected developing system; acellular anatomical structure; hypodermal muscle of larval abdomen. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: EGF-like, conserved site; Epidermal growth factor-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval fat body, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627453_s_at completely aligns to an exonic region common to each of the 7 FlyBase-annotated transcript isoforms of spi. Gene sequence location is 2L:19567900..19577315.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spi: spitz Embryonic lethal. Normal allele required for normal development of blastoderm cells just lateral to the ventral mesodermal precursors. Denticle bands narrower than normal; first row often missing; reversal of polarity between anterior and posterior rows not seen; irregular fusion in midline of adjacent dentical bands. Keilin's organs missing or strongly reduced. Labrum, antennal-maxillary complex and labial sense organ reduced in size; right and left halves of head skeleton fused to produce pointed appearance. Tail region normal. Right and left halves of ventral ganglia of the central nervous system closer together than normal resulting in shorter commissures. The cells in the CNS that stain with anti-eve+ antibodies are closer together in spi than in wild type. spi+ required in the female germ line; spi pole cells transplanted into normal embryos produce no progeny.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 37F2-37F2   Limits computationally determined from genome sequence between P{EP}EP623&P{PZ}spi01068 and P{lacW}l(2)k10239k10239
Experimentally Determined Cytological Location
Cytogenetic map Notes References 37F1-37F2   (determined by in situ hybridisation)   (Spradling et al., 1999) 37F-37F     (Calvi et al., 1998) 37F1-37F2   (determined by in situ hybridisation)   (Perrimon et al., 1996) 38A-38A   (determined by in situ hybridisation)   (Kania et al., 1995) 37F-38A   (determined by in situ hybridisation)   (Tio et al., 1994) 37F1-37F2     (BDGP Project Members, 1994-1999) 37F-38A   (determined by in situ hybridisation)   (Rutledge et al., 1992)
Experimentally Determined Recombination Data
Location	2-54 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spi for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spi-RA FBtr0081268  NM_134291   1653   234 spi-RB FBtr0081269  NM_134293   1634   234 spi-RE FBtr0081270  NM_134292   1438   234 spi-RF FBtr0081267  NM_057561   1457   234 spi-RG FBtr0100597  NM_001032110   1576   234
Additional Transcript Data & Comments
Reported size (kB)	2.5, 2.0 (northern blot) (Rutledge et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spi-PA   FBpp0080809   26.4   234   7.54     NP_599118   AAF53831 spi-PB   FBpp0080810   26.4   234   7.54     NP_599120   AAG22442 spi-PE   FBpp0080811   26.4   234   7.54     NP_599119   AAN11052 spi-PF   FBpp0080808   26.4   234   7.54     NP_476909   AAN11053 spi-PG   FBpp0100054   26.4   234   7.54     NP_001027281   AAZ66477
Additional Polypeptide Data & Comments
Reported size (kDa)	230 (aa); 26 (kD) (Rutledge et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Epidermal growth factor-like domain (IPR000742) EGF-like, conserved site (IPR013032)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201448   AA247046   AA438721   AA978455   AE014134 AAF53831   AE014134 AAG22442   AE014134 AAN11052   AE014134 AAN11053   AE014134 AAZ66477   AC005128   AI062056   AI063044   AI108506   AI114351   AI134122   AI134774   AI294122   AI387575   AI403295   AI514252   AI532159   AI532794   AQ073391   AQ073742   AQ073946   AQ254855   AX094034   AY089693 AAL90431   BG633450   BG635682   BG635952   BG640183   BG640811   BI161727   BI161728   BI166071   BI168272   BI170426   BI173403   BI229299   BI229516   BI236311   BI357251   BI361709   BI362492   BI363234   BI369323   BI370205   BI482146   BI483575   BI573447   BI573448   BI604260   BI612280   BI613693   BI617993   BI623152   BI628817   BI633012   BI635844   BI637027   BI637197   BI639961   BI641057   BP544654   BP544655   BT044503   CL943613   CO180297   CO183986   CO268259   CO274052   CO286995   CO287880   CO290956   CO295405   CO295624   CO298405   CO298754   CO299844   CO304067   CO304230   CO309942   CO309945   CO312308   CO316357   CO322535   CO328403   CO333899   CO341027   CO341270   CZ473487   CZ473488   CZ475768   CZ485801   EC062427   EC064807   EC073742   EC075506   EC077956   EC078379   EC078724   EC081814   EC082420   EC083468   EC087657   EC196331   EC202202   EC212984   EC223719   EC225039   EC225553   EC230131   EC241981   EC244146   EC246647   EC251511   EC253459   EC260333   G00577   GH941296   GH941588   GH941911   GH942639   GH943984   GH944538   GH945020   GH945059   GH945091   GH945302   GH945347   GH945941   GH946463   GH946810   GH946861   GH946922   GH947055   GH947123   GH948758   GH948872   GH949608   GH950351   GH951822   GH952377   GH952856   GH952944   GH954696   GH955038   GH955141   GH955506   GH956182   GH956440   GH956512   GH956756   GH957464   GH957958   GH958024   GH958106   GH958541   GH958570   GH959414   GH960472   GH960687   GH961086   GH961630   GH961631   GH961797   GH961801   GH962145   GH963098   GH963133   GH963858   GH963965   GH964154   GH964454   GH964491   GH964694   GH964724   GH964761   GH964801   GH964821   GH965332   GH965419   GH965688   GH967030   GH967668   GH968551   GH969898   GH970302   GH971251   GH971344   GH971965   GH972151   GH972626   GH972762   GH972789   GH973370   GH974120   GH975477   GH976527   GH976845   GH977554   GH977578   GH978905   GH979482   GH979953   GH980380   GH980492   GH980615   GH980636   GH980700   GH980896   GH981111   GH981134   GH981136   GH981370   GH981497   GH981924   GH982051   GH982278   GH983154   GH983404   GH984352   GH984380   GH984884   M95199 AAA28894
UniProtKB/Swiss-Prot	Q01083
UniProtKB/TrEMBL	A4V0W1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral midline   (Rutledge et al., 1992) mesoderm   (Rutledge et al., 1992) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   peripheral nervous system   (Rutledge et al., 1992) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium organism | ubiquitous   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic dorsal epidermis   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012)
Additional Descriptive Data
	spi transcripts are expressed throughout development with a peak in mid-embryogenesis. In situ hybridizations show that spi is expressed ubiquitously in all embryonic tissues, with enrichment in the procephalic region, ventral midline, mesodermal layers, and PNS. (Rutledge et al., 1992)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   mesoderm   (Rutledge et al., 1992) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral midline   (Rutledge et al., 1992) pupal stage P6   cone cell   (Miller and Cagan, 1998) interommatidial bristle | precursor   (Miller and Cagan, 1998) primary pigment cell   (Miller and Cagan, 1998)
Additional Descriptive Data
	Expression of spi is highest in cone cells and bristle organules (interommatidial bristle precursors), and lower in the primary pigment cells. (Miller and Cagan, 1998) The spi protein is expressed ubiquitously in embryos with slight enrichment in the mesoderm and the ventral midline. (Rutledge et al., 1992)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Lee et al., 2001, Schlesinger et al., 2004) Golgi apparatus inferred from genetic interaction with S (Lee et al., 2001)
Expression Deduced from Reporters
Reporter: P{PZ}spi01068 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval hindgut   (Jackle and Janning, 1998.8.10) embryonic/larval brain   (Jackle and Janning, 1998.8.10) embryonic/larval midgut   (Jackle and Janning, 1998.8.10) embryonic/larval foregut   (Jackle and Janning, 1998.8.10) embryonic/larval optic lobe   (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system | restricted   (Jackle and Janning, 1998.8.10) presumptive embryonic/larval peripheral nervous system | segmentally repeated   (Jackle and Janning, 1998.8.10) wandering third instar larval stage   imaginal disc   (Meister and Braun, 1995.10) embryonic/larval brain   (Meister and Braun, 1995.10) adult stage   optic lobe   (Vosshall, 1998.8.10) adult central complex   (Vosshall, 1998.8.10) adult brain | restricted   (Vosshall, 1998.8.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval fat body, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1011.75 Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body    836.5 Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head    508.9 Adult Eye    535.725 Adult Brain    928.7 Adult Thoracic-Abdominal Ganglion    1203 Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland    548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1011.75) Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body  (836.5) Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head  508.9 Adult Eye  535.725 Adult Brain  (928.7) Adult Thoracic-Abdominal Ganglion  (1203) Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland  (548) Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1011.75 Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body    836.5 Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head    508.9 Adult Eye    535.725 Adult Brain    928.7 Adult Thoracic-Abdominal Ganglion  (1203) Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland    548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1011.75 Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body    836.5 Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head    508.9 Adult Eye    535.725 Adult Brain    928.7 Adult Thoracic-Abdominal Ganglion    1203 Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland    548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1011.75 Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body    836.5 Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head    508.9 Adult Eye    535.725 Adult Brain    928.7 Adult Thoracic-Abdominal Ganglion    1203 Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland    548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1011.75) Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body  (836.5) Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head  508.9 Adult Eye  535.725 Adult Brain  (928.7) Adult Thoracic-Abdominal Ganglion  (1203) Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland  548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1011.75 Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body    836.5 Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head    508.9 Adult Eye    535.725 Adult Brain    928.7 Adult Thoracic-Abdominal Ganglion  1203 Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland    548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1011.75 Larval Midgut    149.1 Larval Hindgut    198.2 Larval Malpighian Tubules    222.9 Larval Fat Body    836.5 Larval Salivary Gland    106.1 Larval Trachea    315.75 Larval Carcass    173.175 Adult Head    508.9 Adult Eye    535.725 Adult Brain    928.7 Adult Thoracic-Abdominal Ganglion    1203 Adult Crop    402.8 Adult Midgut    262.3 Adult Hindgut    286.6 Adult Malpighian Tubules    298.4 Adult Fat Body    142.5 Adult Salivary Gland    548 Adult Heart    262.1 Adult VirginFemale Spermatheca    250.5 Adult InseminatedFemale Spermatheca    257.2 Adult Ovary    275.4 Adult Testis    169.6 Adult Male Accessory Gland    183.7 Adult Carcass    205.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    508.9   NA  Eye    535.725   NA  Brain    928.7   1011.75  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1203   NA  Crop    402.8   149.1  Midgut    262.3   198.2  Hindgut    286.6   222.9  Malpighian Tubules    298.4   836.5  Fat Body    142.5   106.1  Salivary Gland    548   NA  Heart    262.1   315.75  Trachea    NA   NA  VirginFemale Spermatheca    250.5   NA  InseminatedFemale Spermatheca    257.2   NA  Ovary    275.4   NA  Testis    169.6   NA  Male Accessory Gland    183.7   173.175  Carcass    205.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    120 central nervous system, larvae L3    232 central nervous system, pupae P8    230 head, virgin 1-day female    64 head, virgin 4-day female    80 head, virgin 20-day female    46 head, mated 1-day female    63 head, mated 4-day female    58 head, mated 20-day female    49 head, mated 1-day male    101 head, mated 4-day male    107 head, mated 20-day male    118 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    48 digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult    30 fat body, larvae L3 wandering    24 fat body, white prepupae    37 fat body, pupae P8    95 carcass, larvae L3 wandering    111 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    29 ovary, virgin 4-day female    53 ovary, mated 4-day female    43 testis, mated 4-day male    51 accessory gland, mated 4-day male    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (120) central nervous system, larvae L3  (232) central nervous system, pupae P8  (230) head, virgin 1-day female  (64) head, virgin 4-day female  (80) head, virgin 20-day female  (46) head, mated 1-day female  (63) head, mated 4-day female  (58) head, mated 20-day female  (49) head, mated 1-day male  (101) head, mated 4-day male  (107) head, mated 20-day male  (118) salivary gland, larvae L3 wandering    11 salivary gland, white prepupae  (27) digestive system, larvae L3 wandering  (48) digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult  (30) fat body, larvae L3 wandering    24 fat body, white prepupae  (37) fat body, pupae P8  (95) carcass, larvae L3 wandering  (111) carcass, 1-day adult    23 carcass, 4-day adult  (27) carcass, 20-day adult  (29) ovary, virgin 4-day female  (53) ovary, mated 4-day female  (43) testis, mated 4-day male  (51) accessory gland, mated 4-day male  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (120) central nervous system, larvae L3  (232) central nervous system, pupae P8  (230) head, virgin 1-day female    64 head, virgin 4-day female    80 head, virgin 20-day female    46 head, mated 1-day female    63 head, mated 4-day female    58 head, mated 20-day female    49 head, mated 1-day male  101 head, mated 4-day male  107 head, mated 20-day male  (118) salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    48 digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult    30 fat body, larvae L3 wandering    24 fat body, white prepupae    37 fat body, pupae P8    95 carcass, larvae L3 wandering  (111) carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    29 ovary, virgin 4-day female    53 ovary, mated 4-day female    43 testis, mated 4-day male    51 accessory gland, mated 4-day male    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    120 central nervous system, larvae L3    232 central nervous system, pupae P8    230 head, virgin 1-day female    64 head, virgin 4-day female    80 head, virgin 20-day female    46 head, mated 1-day female    63 head, mated 4-day female    58 head, mated 20-day female    49 head, mated 1-day male    101 head, mated 4-day male    107 head, mated 20-day male    118 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    48 digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult    30 fat body, larvae L3 wandering    24 fat body, white prepupae    37 fat body, pupae P8    95 carcass, larvae L3 wandering    111 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    29 ovary, virgin 4-day female    53 ovary, mated 4-day female    43 testis, mated 4-day male    51 accessory gland, mated 4-day male    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    120 central nervous system, larvae L3    232 central nervous system, pupae P8    230 head, virgin 1-day female    64 head, virgin 4-day female    80 head, virgin 20-day female    46 head, mated 1-day female    63 head, mated 4-day female    58 head, mated 20-day female    49 head, mated 1-day male    101 head, mated 4-day male    107 head, mated 20-day male    118 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    48 digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult    30 fat body, larvae L3 wandering    24 fat body, white prepupae    37 fat body, pupae P8    95 carcass, larvae L3 wandering    111 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    29 ovary, virgin 4-day female    53 ovary, mated 4-day female    43 testis, mated 4-day male    51 accessory gland, mated 4-day male    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (120) central nervous system, larvae L3  (232) central nervous system, pupae P8  (230) head, virgin 1-day female  (64) head, virgin 4-day female  (80) head, virgin 20-day female  (46) head, mated 1-day female  (63) head, mated 4-day female  (58) head, mated 20-day female  (49) head, mated 1-day male  (101) head, mated 4-day male  (107) head, mated 20-day male  (118) salivary gland, larvae L3 wandering    11 salivary gland, white prepupae  27 digestive system, larvae L3 wandering  (48) digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult  30 fat body, larvae L3 wandering    24 fat body, white prepupae  (37) fat body, pupae P8  (95) carcass, larvae L3 wandering  (111) carcass, 1-day adult    23 carcass, 4-day adult  27 carcass, 20-day adult  29 ovary, virgin 4-day female  (53) ovary, mated 4-day female  (43) testis, mated 4-day male  (51) accessory gland, mated 4-day male  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  120 central nervous system, larvae L3  (232) central nervous system, pupae P8  (230) head, virgin 1-day female    64 head, virgin 4-day female    80 head, virgin 20-day female    46 head, mated 1-day female    63 head, mated 4-day female    58 head, mated 20-day female    49 head, mated 1-day male  101 head, mated 4-day male  107 head, mated 20-day male  118 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    48 digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult    30 fat body, larvae L3 wandering    24 fat body, white prepupae    37 fat body, pupae P8    95 carcass, larvae L3 wandering  111 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    29 ovary, virgin 4-day female    53 ovary, mated 4-day female    43 testis, mated 4-day male    51 accessory gland, mated 4-day male    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    120 central nervous system, larvae L3    232 central nervous system, pupae P8    230 head, virgin 1-day female    64 head, virgin 4-day female    80 head, virgin 20-day female    46 head, mated 1-day female    63 head, mated 4-day female    58 head, mated 20-day female    49 head, mated 1-day male    101 head, mated 4-day male    107 head, mated 20-day male    118 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    48 digestive system, 1-day adult    18 digestive system, 4-day adult    22 digestive system, 20-day adult    30 fat body, larvae L3 wandering    24 fat body, white prepupae    37 fat body, pupae P8    95 carcass, larvae L3 wandering    111 carcass, 1-day adult    23 carcass, 4-day adult    27 carcass, 20-day adult    29 ovary, virgin 4-day female    53 ovary, mated 4-day female    43 testis, mated 4-day male    51 accessory gland, mated 4-day male    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    83 embryo 04-06hr    249 embryo 06-08hr    258 embryo 08-10hr    165 embryo 10-12hr    136 embryo 12-14hr    102 embryo 14-16hr    60 embryo 16-18hr    41 embryo 18-20hr    37 embryo 20-22hr    30 embryo 22-24hr    38 larva L1    35 larva L2    30 larva L3 12hr old    17 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    60 larva L3 puffstage 7-9    103 white prepupae new    79 white prepupae 12hr    112 white prepupae 24hr    117 pupae 2d postWPP    94 pupae 3d postWPP    36 pupae 4d postWPP    23 adult male 01day    26 adult male 05day    31 adult male 30day    34 adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr  (83) embryo 04-06hr  (249) embryo 06-08hr  (258) embryo 08-10hr  (165) embryo 10-12hr  (136) embryo 12-14hr  (102) embryo 14-16hr  (60) embryo 16-18hr  (41) embryo 18-20hr  (37) embryo 20-22hr  (30) embryo 22-24hr  (38) larva L1  (35) larva L2  (30) larva L3 12hr old    17 larva L3 puffstage 1-2  (29) larva L3 puffstage 3-6  (60) larva L3 puffstage 7-9  (103) white prepupae new  (79) white prepupae 12hr  (112) white prepupae 24hr  (117) pupae 2d postWPP  (94) pupae 3d postWPP  (36) pupae 4d postWPP    23 adult male 01day  26 adult male 05day  (31) adult male 30day  (34) adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    83 embryo 04-06hr  (249) embryo 06-08hr  (258) embryo 08-10hr  (165) embryo 10-12hr  (136) embryo 12-14hr  102 embryo 14-16hr    60 embryo 16-18hr    41 embryo 18-20hr    37 embryo 20-22hr    30 embryo 22-24hr    38 larva L1    35 larva L2    30 larva L3 12hr old    17 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    60 larva L3 puffstage 7-9  103 white prepupae new    79 white prepupae 12hr  (112) white prepupae 24hr  (117) pupae 2d postWPP    94 pupae 3d postWPP    36 pupae 4d postWPP    23 adult male 01day    26 adult male 05day    31 adult male 30day    34 adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    83 embryo 04-06hr    249 embryo 06-08hr    258 embryo 08-10hr    165 embryo 10-12hr    136 embryo 12-14hr    102 embryo 14-16hr    60 embryo 16-18hr    41 embryo 18-20hr    37 embryo 20-22hr    30 embryo 22-24hr    38 larva L1    35 larva L2    30 larva L3 12hr old    17 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    60 larva L3 puffstage 7-9    103 white prepupae new    79 white prepupae 12hr    112 white prepupae 24hr    117 pupae 2d postWPP    94 pupae 3d postWPP    36 pupae 4d postWPP    23 adult male 01day    26 adult male 05day    31 adult male 30day    34 adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    83 embryo 04-06hr    249 embryo 06-08hr    258 embryo 08-10hr    165 embryo 10-12hr    136 embryo 12-14hr    102 embryo 14-16hr    60 embryo 16-18hr    41 embryo 18-20hr    37 embryo 20-22hr    30 embryo 22-24hr    38 larva L1    35 larva L2    30 larva L3 12hr old    17 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    60 larva L3 puffstage 7-9    103 white prepupae new    79 white prepupae 12hr    112 white prepupae 24hr    117 pupae 2d postWPP    94 pupae 3d postWPP    36 pupae 4d postWPP    23 adult male 01day    26 adult male 05day    31 adult male 30day    34 adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr  (83) embryo 04-06hr  (249) embryo 06-08hr  (258) embryo 08-10hr  (165) embryo 10-12hr  (136) embryo 12-14hr  (102) embryo 14-16hr  (60) embryo 16-18hr  (41) embryo 18-20hr  (37) embryo 20-22hr  30 embryo 22-24hr  (38) larva L1  (35) larva L2  30 larva L3 12hr old    17 larva L3 puffstage 1-2  29 larva L3 puffstage 3-6  (60) larva L3 puffstage 7-9  (103) white prepupae new  (79) white prepupae 12hr  (112) white prepupae 24hr  (117) pupae 2d postWPP  (94) pupae 3d postWPP  (36) pupae 4d postWPP    23 adult male 01day  26 adult male 05day  31 adult male 30day  (34) adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    83 embryo 04-06hr  (249) embryo 06-08hr  (258) embryo 08-10hr  (165) embryo 10-12hr  136 embryo 12-14hr  102 embryo 14-16hr    60 embryo 16-18hr    41 embryo 18-20hr    37 embryo 20-22hr    30 embryo 22-24hr    38 larva L1    35 larva L2    30 larva L3 12hr old    17 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    60 larva L3 puffstage 7-9  103 white prepupae new    79 white prepupae 12hr  112 white prepupae 24hr  117 pupae 2d postWPP    94 pupae 3d postWPP    36 pupae 4d postWPP    23 adult male 01day    26 adult male 05day    31 adult male 30day    34 adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    83 embryo 04-06hr    249 embryo 06-08hr    258 embryo 08-10hr    165 embryo 10-12hr    136 embryo 12-14hr    102 embryo 14-16hr    60 embryo 16-18hr    41 embryo 18-20hr    37 embryo 20-22hr    30 embryo 22-24hr    38 larva L1    35 larva L2    30 larva L3 12hr old    17 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    60 larva L3 puffstage 7-9    103 white prepupae new    79 white prepupae 12hr    112 white prepupae 24hr    117 pupae 2d postWPP    94 pupae 3d postWPP    36 pupae 4d postWPP    23 adult male 01day    26 adult male 05day    31 adult male 30day    34 adult female 01day    21 adult female 05day    18 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    498 Schneider line 2 Sg4    258 embryonic 1182-4H    473 embryonic GM2    270 embryonic Kc167    432 embryonic S1    86 embryonic S3    264 leg disc CME L1    196 wing disc CME-W2    207 wing disc ML-DmD8    173 wing disc ML-DmD9    143 wing disc ML-DmD16-c3    242 wing disc ML-DmD21    185 wing disc ML-DmD32    468 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    88 antennal disc ML-DmD20-c5    196 mixed discs ML-DmD4-c1    157 CNS ML-DmBG1-c1    173 CNS ML-DmBG2-c2    241 tumorous blood cells mbn2    427 ovary fGS/OSS    153 ovary OSC    261 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (498) Schneider line 2 Sg4  (258) embryonic 1182-4H  (473) embryonic GM2  (270) embryonic Kc167  (432) embryonic S1  (86) embryonic S3  (264) leg disc CME L1  (196) wing disc CME-W2  (207) wing disc ML-DmD8  (173) wing disc ML-DmD9  (143) wing disc ML-DmD16-c3  (242) wing disc ML-DmD21  (185) wing disc ML-DmD32  (468) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11  (88) antennal disc ML-DmD20-c5  (196) mixed discs ML-DmD4-c1  (157) CNS ML-DmBG1-c1  (173) CNS ML-DmBG2-c2  (241) tumorous blood cells mbn2  (427) ovary fGS/OSS  (153) ovary OSC  (261) ovary OSS  (85) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (498) Schneider line 2 Sg4  (258) embryonic 1182-4H  (473) embryonic GM2  (270) embryonic Kc167  (432) embryonic S1    86 embryonic S3  (264) leg disc CME L1  (196) wing disc CME-W2  (207) wing disc ML-DmD8  (173) wing disc ML-DmD9  (143) wing disc ML-DmD16-c3  (242) wing disc ML-DmD21  (185) wing disc ML-DmD32  (468) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11    88 antennal disc ML-DmD20-c5  (196) mixed discs ML-DmD4-c1  (157) CNS ML-DmBG1-c1  (173) CNS ML-DmBG2-c2  (241) tumorous blood cells mbn2  (427) ovary fGS/OSS  (153) ovary OSC  (261) ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    498 Schneider line 2 Sg4    258 embryonic 1182-4H    473 embryonic GM2    270 embryonic Kc167    432 embryonic S1    86 embryonic S3    264 leg disc CME L1    196 wing disc CME-W2    207 wing disc ML-DmD8    173 wing disc ML-DmD9    143 wing disc ML-DmD16-c3    242 wing disc ML-DmD21    185 wing disc ML-DmD32    468 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    88 antennal disc ML-DmD20-c5    196 mixed discs ML-DmD4-c1    157 CNS ML-DmBG1-c1    173 CNS ML-DmBG2-c2    241 tumorous blood cells mbn2    427 ovary fGS/OSS    153 ovary OSC    261 ovary OSS    85 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    498 Schneider line 2 Sg4    258 embryonic 1182-4H    473 embryonic GM2    270 embryonic Kc167    432 embryonic S1    86 embryonic S3    264 leg disc CME L1    196 wing disc CME-W2    207 wing disc ML-DmD8    173 wing disc ML-DmD9    143 wing disc ML-DmD16-c3    242 wing disc ML-DmD21    185 wing disc ML-DmD32    468 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    88 antennal disc ML-DmD20-c5    196 mixed discs ML-DmD4-c1    157 CNS ML-DmBG1-c1    173 CNS ML-DmBG2-c2    241 tumorous blood cells mbn2    427 ovary fGS/OSS    153 ovary OSC    261 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (498) Schneider line 2 Sg4  (258) embryonic 1182-4H  (473) embryonic GM2  (270) embryonic Kc167  (432) embryonic S1  (86) embryonic S3  (264) leg disc CME L1  (196) wing disc CME-W2  (207) wing disc ML-DmD8  (173) wing disc ML-DmD9  (143) wing disc ML-DmD16-c3  (242) wing disc ML-DmD21  (185) wing disc ML-DmD32  (468) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11  (88) antennal disc ML-DmD20-c5  (196) mixed discs ML-DmD4-c1  (157) CNS ML-DmBG1-c1  (173) CNS ML-DmBG2-c2  (241) tumorous blood cells mbn2  (427) ovary fGS/OSS  (153) ovary OSC  (261) ovary OSS  (85) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (498) Schneider line 2 Sg4  (258) embryonic 1182-4H  (473) embryonic GM2  (270) embryonic Kc167  (432) embryonic S1    86 embryonic S3  (264) leg disc CME L1  (196) wing disc CME-W2  (207) wing disc ML-DmD8  (173) wing disc ML-DmD9  143 wing disc ML-DmD16-c3  (242) wing disc ML-DmD21  (185) wing disc ML-DmD32  (468) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11    88 antennal disc ML-DmD20-c5  (196) mixed discs ML-DmD4-c1  (157) CNS ML-DmBG1-c1  (173) CNS ML-DmBG2-c2  (241) tumorous blood cells mbn2  (427) ovary fGS/OSS  (153) ovary OSC  (261) ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    498 Schneider line 2 Sg4    258 embryonic 1182-4H    473 embryonic GM2    270 embryonic Kc167    432 embryonic S1    86 embryonic S3    264 leg disc CME L1    196 wing disc CME-W2    207 wing disc ML-DmD8    173 wing disc ML-DmD9    143 wing disc ML-DmD16-c3    242 wing disc ML-DmD21    185 wing disc ML-DmD32    468 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    88 antennal disc ML-DmD20-c5    196 mixed discs ML-DmD4-c1    157 CNS ML-DmBG1-c1    173 CNS ML-DmBG2-c2    241 tumorous blood cells mbn2    427 ovary fGS/OSS    153 ovary OSC    261 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    35 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    67 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    92 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    84 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    26 Caffeine 1.5 mg/ml 4 hrs, larvae L3    39 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    63 Paraquat 10 mM 48 hrs, 4-day adult    55 Rotenone 2 μg 12 hrs, larvae L3    36 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (42) cold shock, 4-day adult  (35) heat shock, 4-day adult  26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3  (67) Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (75) Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult  (92) Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult  (84) Ethanol 2.5% 3 hrs, larvae L3  (38) Ethanol 5% 3 hrs, larvae L3  (42) Ethanol 10% 3 hrs, larvae L3  26 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (39) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (82) Caffeine 25 mg/ml 48 hrs, 4-day adult  (44) Paraquat 5 mM 48 hrs, 4-day adult  (63) Paraquat 10 mM 48 hrs, 4-day adult  (55) Rotenone 2 μg 12 hrs, larvae L3  (36) Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    35 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    67 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    92 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    84 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    26 Caffeine 1.5 mg/ml 4 hrs, larvae L3    39 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    63 Paraquat 10 mM 48 hrs, 4-day adult    55 Rotenone 2 μg 12 hrs, larvae L3    36 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    35 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    67 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    92 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    84 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    26 Caffeine 1.5 mg/ml 4 hrs, larvae L3    39 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    63 Paraquat 10 mM 48 hrs, 4-day adult    55 Rotenone 2 μg 12 hrs, larvae L3    36 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    35 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    67 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    92 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    84 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    26 Caffeine 1.5 mg/ml 4 hrs, larvae L3    39 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    63 Paraquat 10 mM 48 hrs, 4-day adult    55 Rotenone 2 μg 12 hrs, larvae L3    36 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (42) cold shock, 4-day adult  (35) heat shock, 4-day adult  26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3  (67) Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (75) Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult  (92) Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult  (84) Ethanol 2.5% 3 hrs, larvae L3  (38) Ethanol 5% 3 hrs, larvae L3  (42) Ethanol 10% 3 hrs, larvae L3  26 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (39) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (82) Caffeine 25 mg/ml 48 hrs, 4-day adult  (44) Paraquat 5 mM 48 hrs, 4-day adult  (63) Paraquat 10 mM 48 hrs, 4-day adult  (55) Rotenone 2 μg 12 hrs, larvae L3  (36) Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    35 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    67 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    92 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    84 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    26 Caffeine 1.5 mg/ml 4 hrs, larvae L3    39 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    63 Paraquat 10 mM 48 hrs, 4-day adult    55 Rotenone 2 μg 12 hrs, larvae L3    36 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    35 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    67 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    92 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    84 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    42 Ethanol 10% 3 hrs, larvae L3    26 Caffeine 1.5 mg/ml 4 hrs, larvae L3    39 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    63 Paraquat 10 mM 48 hrs, 4-day adult    55 Rotenone 2 μg 12 hrs, larvae L3    36 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_11 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • RH69567 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005672 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10334-RF FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for spi Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal spiGD1779 lethal, with Scer\GAL4cb41 spis.Scer\UAS lethal, with Scer\GAL4h-H10 spis.Scer\UAS lethal, with Scer\GAL4Kr.PM spiScer\UAS.cSa lethal | embryonic stage spi2 spi37Fa-2 spi37Fa-3 lethal | embryonic stage | recessive spis3547 spiunspecified lethal | female | pupal stage, with Scer\GAL471B spis.Scer\UAS lethal | larval stage, with Scer\GAL4hs.PB spis.Scer\UAS lethal | male | pharate adult stage, with Scer\GAL471B spis.Scer\UAS lethal | pupal stage, with Scer\GAL4GMR.PF spis.Scer\UAS lethal | recessive spi133a spi01068 spi07132 spi10681 spik05808 spis3547 spiSC1 spiSC2 spiSC3 viable spiEY14184 spiSCP1 spiSCP2 viable, with Scer\GAL4pnr-MD237 spiGD1779 viable | poor spiSCP1 spiSCP2 Sterility Allele fertile spiEY14184 Other Phenotypes Allele cell death defective | somatic clone | third instar larval stage spi1 chemical resistant, with Scer\GAL4tub.PU spisec.Scer\UAS.P\T decreased cell number, with Scer\GAL4nos.UTR.T:Hsim\VP16 spis.Scer\UAS increased cell death spi1 increased cell death | embryonic stage spi1 increased cell death | somatic clone spi1 increased cell number, with Scer\GAL4nos.UTR.T:Hsim\VP16 spiRNAi.Scer\UAS increased cell number | third instar larval stage, with Scer\GAL4esg-NP7397 spis.Scer\UAS mitotic cell cycle defective, with Scer\GAL4GMR.PF spis.Scer\UAS neuroanatomy defective spi1 spiGR883 spiIDB7 neuroanatomy defective | embryonic stage spi1 spi2 neuroanatomy defective | embryonic stage 13, with Scer\GAL4sim.P3.7 spis.Scer\UAS neuroanatomy defective | embryonic stage 16, with Scer\GAL4sim.P3.7 spis.Scer\UAS neuroanatomy defective | larval stage spiGD1779 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 spiEP2632 visible spis.sev spiSCP1 spiSCP2 visible, with Scer\GAL4bs-1348 spis.Scer\UAS visible, with Scer\GAL4GMR.PF spis.Scer\UAS visible, with Scer\GAL4kni.L2 spis.Scer\UAS visible | dominant spis3547 visible | female, with Scer\GAL471B spis.Scer\UAS visible | homeotic, with Scer\GAL4ey.PB spis.Scer\UAS visible | recessive spiSCP1 wild-type spi+t12.5 spis.sev Phenotype manifest in Allele A1-7 ventral acute muscle 2 | precursor spi3 abdominal 1 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 1 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal 2 dorsal acute muscle 1 spi1 abdominal 2 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 2 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal 3 dorsal acute muscle 1 spi1 abdominal 3 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 3 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal 4 dorsal acute muscle 1 spi1 abdominal 4 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 4 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal 5 dorsal acute muscle 1 spi1 abdominal 5 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 5 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal 6 dorsal acute muscle 1 spi1 abdominal 6 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 6 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal 7 dorsal acute muscle 1 spi1 abdominal 7 lateral pentascolopidial chordotonal organ lch5 spiunspecified abdominal 7 ventral monoscolopidial chordotonal organ vch1 spiunspecified abdominal dorso-lateral adult muscle precursor cell spi1 abdominal lateral adult muscle precursor cell spi1 abdominal lateral pentascolopidial chordotonal organ lch5 spi1 abdominal lateral pentascolopidial chordotonal organ lch5 | supernumerary, with Scer\GAL4en-e16E spis.Scer\UAS aCC neuron, with Scer\GAL4sim.PS spis.Scer\UAS adult heart spiSPC2 adult midgut precursor cell | third instar larval stage, with Scer\GAL4esg-NP7397 spis.Scer\UAS adult salivary gland primordium spi1 anal pad spiunspecified antenna | ectopic, with Scer\GAL4ey.PB spis.Scer\UAS anterior commissure spi1 spiGR883 spiIDB7 anterior fascicle & synapse, with Scer\GAL4elav-C155 spiEP2632 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 spiEP2632 anterior fascicle sensory neuron spi1 Bolwig organ spi1 border follicle cell, with Scer\GAL4slbo.2.6 spis.Scer\UAS spisec.Scer\UAS.P\T border follicle cell | somatic clone, with Scer\GAL4cb41 spis.Scer\UAS bract & leg | ectopic, with Scer\GAL4sca-537.4 spis.Scer\UAS bract | ectopic, with Scer\GAL4hs.PB spis.Scer\UAS bract | ectopic, with Scer\GAL4neur-GAL4-A101 spis.Scer\UAS.T:Avic\GFP-EGFP bract | somatic clone spi1 cephalopharyngeal skeleton spiunspecified chorion, with Scer\GAL4otu.PR spiScer\UAS.P\T.cGa spisec.Scer\UAS.P\T commissure spiIIB spiunspecified cone cell spis.sev denticle, with Scer\GAL469B spis.Scer\UAS denticle, with Scer\GAL4e22c spis.Scer\UAS denticle | ectopic, with Scer\GAL4arm.PS spis.Scer\UAS denticle | ectopic, with Scer\GAL4en-e16E spis.Scer\UAS denticle belt spi1 spiunspecified denticle belt, with Scer\GAL455B spis.Scer\UAS denticle belt, with Scer\GAL4arm.PS spis.Scer\UAS denticle belt | embryonic stage spis3547 denticle belt | ventral spi1 spiVA17 denticle row 5, with Scer\GAL4arm.PS spis.Scer\UAS denticle row 6, with Scer\GAL4arm.PS spis.Scer\UAS dorsal appendage, with Scer\GAL455B spis.Scer\UAS dorsal hair, with Scer\GAL455B spis.Scer\UAS dorsal neurohemal organ spi1 dorsal neurohemal organ, with Scer\GAL4sim.PS spis.Scer\UAS egg | dorsal spi1 spiSCP2 embryonic/first instar larval cuticle spi1 spis3547 embryonic/first instar larval cuticle, with Scer\GAL4en-e16E spis.Scer\UAS embryonic/first instar larval cuticle & denticle, with Scer\GAL4en-e16E spis.Scer\UAS embryonic/larval brain spi1 embryonic/larval cuticle, with Scer\GAL4arm.PS spis.Scer\UAS embryonic/larval ganglionic branch spis3547 embryonic/larval oenocyte spi1 embryonic/larval oenocyte | ectopic, with Scer\GAL4ato.3.6 spis.Scer\UAS embryonic/larval oenocyte | ectopic, with Scer\GAL4en-e16E spis.Scer\UAS embryonic/larval oenocyte | supernumerary, with Scer\GAL4en-e16E spis.Scer\UAS embryonic/larval optic lobe, with Scer\GAL4unspecified spis.Scer\UAS embryonic/larval optic lobe | posterior spi1 embryonic/larval recurrent nerve spi1 embryonic/larval somatic muscle spi1 embryonic/larval stomatogastric nervous system spi2 embryonic/larval trachea spi3 embryonic anterior Malpighian tubule spi2 embryonic epidermis, with Scer\GAL469B spis.Scer\UAS embryonic epidermis, with Scer\GAL4e22c spis.Scer\UAS embryonic epidermis, with Scer\GAL4Kr.PM spis.Scer\UAS embryonic mesothoracic segment, with Scer\GAL4ato.3.6 spis.Scer\UAS embryonic mesothoracic segment, with Scer\GAL4en-e16E spis.Scer\UAS embryonic metathoracic segment, with Scer\GAL4ato.3.6 spis.Scer\UAS embryonic metathoracic segment, with Scer\GAL4en-e16E spis.Scer\UAS embryonic prothoracic segment, with Scer\GAL4ato.3.6 spis.Scer\UAS embryonic prothoracic segment, with Scer\GAL4en-e16E spis.Scer\UAS embryonic segment | posterior compartment spi1 embryonic stomatogastric nervous system spi1 embryonic ventral epidermis spi1 embryonic ventral nervous system spiunspecified epidermis spiGR883 spiIDB7 eye spiSCP1 spiSCP2 spis.sev eye, with Scer\GAL4ey.PB spis.Scer\UAS eye, with Scer\GAL4GMR.PF spis.Scer\UAS eye disc | somatic clone spi1 eye photoreceptor cell spiSCP1 eye photoreceptor cell | ectopic spis.sev eye photoreceptor cell | somatic clone spiSC1 filzkorper, with Scer\GAL4ems.HRE spis.Scer\UAS filzkorper, with Scer\GAL4salm-459.2 spis.Scer\UAS ganglion mother cell | larval stage spiGD1779 genital disc primordium spi2 germline stem cell, with Scer\GAL4nos.UTR.T:Hsim\VP16 spis.Scer\UAS germline stem cell | supernumerary, with Scer\GAL4nos.UTR.T:Hsim\VP16 spiRNAi.Scer\UAS hypocerebral ganglion spi1 intersegmental nerve, with Scer\GAL4elav-C155 spiEP2632 Keilin's organ spiunspecified lamellocyte | larval stage, with Scer\GAL4Dot.PK spis.Scer\UAS lamellocyte | larval stage | conditional, with Scer\GAL4Antp-10 spiNIG.10334R lamina & neuron spi1 spi2 spi3 lamina & neuron, with Scer\GAL4hs.PB spis.Scer\UAS larval sense organ & antennal segment spiunspecified larval sense organ & maxillary segment spiunspecified lch5 cap attachment cell spi3 lch5 ligament attachment cell spi3 lch5 ligament attachment cell | supernumerary, with Scer\GAL4repo spis.Scer\UAS lch5 neuron spi1 longitudinal connective spi1 spiunspecified macrochaeta spiBG00586 Malpighian tubule spi1 spi2 mesothoracic tarsal segment 1 & bract | ectopic, with Scer\GAL4sca-537.4 spis.Scer\UAS mesothoracic tibial apical bristle, with Scer\GAL4Dll-md23 spis.Scer\UAS mesothoracic tibial apical bristle, with Scer\GAL4sca-537.4 spis.Scer\UAS mesothoracic tibial bract | ectopic, with Scer\GAL4sca-537.4 spis.Scer\UAS midgut, with Scer\GAL4esg-NP5130, Scer\GAL80ts.αTub84B spis.Scer\UAS midgut, with Scer\GAL4how-24B, Scer\GAL80ts.αTub84B spiVDRC.cUa midline glial cell spi1 spiunspecified midline glial cell, with Scer\GAL4hs.PB spis.Scer\UAS midline glial cell | embryonic stage 13 | supernumerary, with Scer\GAL4sim.P3.7 spis.Scer\UAS midline glial cell | embryonic stage 16 | supernumerary, with Scer\GAL4sim.P3.7 spis.Scer\UAS midline ventral glial cell spi1 mitotic cell cycle & eye disc | somatic clone spiSC1 motor neuron spiunspecified muscle founder cell | embryonic stage, with Scer\GAL4twi.PG spis.Scer\UAS neuron | ectopic, with Scer\GAL4hs.PB spis.Scer\UAS ommatidium spi1 spiSC1 ommatidium, with Scer\GAL4GMR.PF spis.Scer\UAS ommatidium, with Scer\GAL4hs.PB spis.Scer\UAS ommatidium | somatic clone spiSC2 optic lobe spi1 pCC neuron, with Scer\GAL4sim.PS spis.Scer\UAS peripheral glial cell | embryonic stage spi1 peripheral nervous system spiGR883 spiIDB7 photoreceptor spiSCP1 photoreceptor cell, with Scer\GAL4GMR.PF spis.Scer\UAS photoreceptor cell, with Scer\GAL4hs.PB spis.Scer\UAS photoreceptor cell R1 spi1 spi2 spi3 photoreceptor cell R2 spi1 spi2 spi3 spiSC1 spiSC2 photoreceptor cell R2 | somatic clone spiSC2 photoreceptor cell R3 spi1 spi2 spi3 spiSC1 spiSC2 photoreceptor cell R4 spi1 spi2 spi3 spiSC1 spiSC2 photoreceptor cell R5 spi1 spi2 spi3 spiSC1 spiSC2 photoreceptor cell R5 | somatic clone spiSC2 photoreceptor cell R6 spi1 spi2 spi3 photoreceptor cell R7 spi1 spi2 spi3 photoreceptor cell R7 | ectopic spis.sev photoreceptor cell R8 spi1 spiSC1 spiSC2 photoreceptor cell R8 | somatic clone spi2 pigment cell spis.sev posterior commissure spi1 spiGR883 spiIDB7 posterior fascicle sensory neuron spi1 presumptive embryonic/larval central nervous system spiunspecified presumptive embryonic/larval peripheral nervous system spiunspecified presumptive embryonic/larval peripheral nervous system, with Scer\GAL4en-e16E spis.Scer\UAS presumptive embryonic/larval tracheal system spi2 prothoracic tibial apical bristle, with Scer\GAL4Dll-md23 spis.Scer\UAS prothoracic tibial apical bristle, with Scer\GAL4sca-537.4 spis.Scer\UAS retina, with Scer\GAL4GMR.PF spis.Scer\UAS retina & basal lamina spiSCP1 spiSCP2 rhabdomere spiSCP1 spiSCP2 sensory neuron spiunspecified sensory neuron & axon & embryo spi1 stigmatophore, with Scer\GAL4ems.HRE spis.Scer\UAS stigmatophore, with Scer\GAL4salm-459.2 spis.Scer\UAS stomatogastric nervous system anlage, with Scer\GAL4Act.PU spis.Scer\UAS stomodeal invagination spi2 subretinal glial cell, with Scer\GAL4GMR.PF spis.Scer\UAS taste bristle & leg | ectopic, with Scer\GAL4Dll-md23 spis.Scer\UAS taste bristle & leg | ectopic, with Scer\GAL4sca-537.4 spis.Scer\UAS tracheal primordium spi2 trichome, with Scer\GAL4hs.PB spis.Scer\UAS ventral longitudinal muscle spi1 ventral nerve cord spis3547 ventral nerve cord | dorsal spi1 ventral nerve cord commissure spi1 ventral oblique muscle spi1 spiGR883 spiIDB7 ventral thoracic disc, with Scer\GAL4Dll-md23 spis.Scer\UAS wing, with Scer\GAL4Bx-MS1096 spiΔIC.Scer\UAS.T:Zzzz\FLAG wing, with Scer\GAL4kni.L2 spis.Scer\UAS wing | anterior spis3547 wing | female, with Scer\GAL471B spis.Scer\UAS wing disc, with Scer\GAL471B spis.Scer\UAS wing disc, with Scer\GAL4Dll-md23 spis.Scer\UAS wing disc, with Scer\GAL4dpp.blk1 spis.Scer\UAS wing hair | female, with Scer\GAL471B spis.Scer\UAS wing vein, with Scer\GAL4bs-1348 spis.Scer\UAS wing vein | ectopic, with Scer\GAL4kni.L2 spis.Scer\UAS wing vein | ectopic | female, with Scer\GAL471B spis.Scer\UAS
Classical Alleles ( 49 )
For All Classical Alleles Show
Allele of spi Class Mutagen Stocks Known lesion spi1 loss of function allele ethyl methanesulfonate 2 -- spis3547 P-element activity 2 -- spi2 loss of function allele ethyl methanesulfonate 1 -- spiCB-5296-3 1 -- spiCB-6534-3 1 -- spiCB-6667-3 1 -- spid03659 P-element activity 1 -- spid06955 1 -- spid07490 P-element activity 1 -- spiEP2378 P-element activity 1 -- spiEY14184 P-element activity 1 -- spif03256 piggyBac transposase 1 -- spiGS9657 1 Yes spik05808 P-element activity 1 -- spiNP0261 1 -- spiNP0289 1 -- spiUM-8231-3 1 -- spiUM-8232-3 1 -- spiSC1 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- spiSC2 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- spiSC3 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- spi00233 P-element activity 0 -- spi01068 P-element activity 0 -- spi07132 P-element activity 0 -- spi10681 P-element activity 0 -- spi133a P-element activity 0 Yes spi259 0 -- spi37Fa-2 diepoxybutane 0 -- spi37Fa-3 diepoxybutane 0 -- spi3 ethyl methanesulfonate 0 -- spiBG00586 P-element activity 0 Yes spid13 X ray 0 -- spiDG04705 P-element activity 0 -- spiE55 0 -- spiEP2201 P-element activity 0 -- spiEP2632 P-element activity 0 Yes spiEP3363 P-element activity 0 -- spiGR883 P-element activity 0 Yes spiIDB7 P-element activity 0 Yes spiIIB 0 -- spiIIIM105 0 -- spiNP3582 0 -- spiP1 P-element activity 0 -- spirevIDB7 0 -- spiSCP1 loss of function allele P-element activity 0 Yes spiSCP2 loss of function allele P-element activity 0 Yes spiSPC2 0 -- spiunspecified 0 -- spiVA17 0 --
Alleles Carried on Transgenic Constructs ( 38 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spi Class Mutagen Stocks Known lesion spiGD1779 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes spiHMS01120 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spiJF03322 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spiKK105786 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spi+t12.5 in vitro construct - genomic fragment 0 Yes spic.Scer\UAS.T:Arus\HRP,T:SS-wg in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes spic.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spiCS.m.Scer\UAS in vitro construct - site directed mutagenesis 0 Yes spiCS.s.Scer\UAS.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes spidsRNA.Scer\UAS.cUa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes spifl.MtnA in vitro construct - regulatory fusion 0 Yes spihs.PG in vitro construct - regulatory fusion 0 Yes spim.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes spiMtnA.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spimut.Scer\UAS.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes spiNIG.10334R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes spiNot3 in vitro construct - deletion 0 Yes spiRNAi.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 -- spis.hs in vitro construct - regulatory fusion 0 Yes spis.MtnA.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spis.MtnA in vitro construct - amino acid replacement in vitro construct 0 Yes spis.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes spis.Scer\UAS in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes spis.sev in vitro construct - regulatory fusion 0 Yes spiScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes spiScer\UAS.P\T.cGa in vitro construct - regulatory fusion 0 Yes spiScer\UAS.T:Avic\GFP in vitro construct - coding region fusion 0 Yes spiScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spiScer\UAS.T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes spiScer\UAS.T:Zzzz\TAP in vitro construct - coding region fusion 0 Yes spiScer\UAS.Δ16 in vitro construct - deletion 0 Yes spiScer\UAS.ΔEGF.s.T:Avic\GFP in vitro construct - deletion 0 Yes spiScer\UAS.ΔEGF.T:Avic\GFP in vitro construct - deletion 0 Yes spiScer\UAS.ΔIC.T:Avic\GFP in vitro construct - site directed mutagenesis 0 Yes spisec.Scer\UAS.P\T in vitro construct - regulatory fusion 0 Yes spiVDRC.cUa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes spiΔ16aa.Scer\UAS in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes spiΔIC.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)E55 (Tearle and Nusslein-Volhard, 1987, Jones and McGinnis, 1993, BDGP Project Members, 1994-1999, Tio et al., 1994, Harbecke and Lengyel, 1995) Df(2L)Exel8041 (Ryder, 2004.4.26) Df(2L)OD12 (Schnepp et al., 1996) Df(2L)OD16 (Jones and McGinnis, 1993) Df(2L)pr76 (Harbecke and Lengyel, 1995) Df(2L)pr-A14 (Tio et al., 1994) Df(2L)pr-A16 (Tio et al., 1994) Df(2L)Sd14 (BDGP Project Members, 1994-1999) Df(2L)Sd77 (BDGP Project Members, 1994-1999, Tio et al., 1994) Df(2L)TW2 (BDGP Project Members, 1994-1999) Df(2L)TW9 (Tearle and Nusslein-Volhard, 1987, BDGP Project Members, 1994-1999) Df(2L)TW50 (Spradling et al., 1999, Faucheux et al., 2001, BDGP Project Members, 1994-1999, Tio et al., 1994, Harbecke and Lengyel, 1995) Df(2L)VA12 (Tio et al., 1994) Df(2L)VA17 (BDGP Project Members, 1994-1999, Schnepp et al., 1996) Df(2L)VA19 (BDGP Project Members, 1994-1999) Tp(2;2)dppd13 (Rutledge et al., 1992)
Not disrupted in	Df(2L)pr28 (BDGP Project Members, 1994-1999) Df(2L)pr65 (Jones and McGinnis, 1993) Df(2L)TW65 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)TW84 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)TW150 (BDGP Project Members, 1994-1999) Df(2L)TW158 (BDGP Project Members, 1994-1999) Df(2L)TW161 (Jones and McGinnis, 1993)
Duplicated in	Dp(2;Y)G-M15 (Contamine et al., 1989)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1779} NA P{KK105786} NA P{NIG.10334R} NA P{TRiP.HMS01120} NA P{TRiP.JF03322} NA P{UAS-cSpi.HRP} NA P{UAS-mSpi.CS} NA P{UAS-myc-spi.L} NA P{UASp-spi.G} NA P{UASp-spi.sec} NA P{UAS-spi.c.HA} NA P{UAS-spi.FLAG} NA P{UAS-spi.m.GFP} NA P{UAS-spi.m.HA} NA P{UAS-spi.m} NA P{UAS-spi.mut.MYC} NA P{UAS-spi.RNAi.U} NA P{UAS-spi.s.GFP} NA P{UAS-spi.s.T:GFP} NA P{UAS-spi.s} NA P{UAS-spi.T:GFP} NA P{UAS-spi.TAP} NA P{UAS-spi.Δ16} NA P{UAS-spi.Δ16aa} NA P{UAS-Spi.ΔIC.FLAG} NA P{UAS-spi.ΔIC.T:GFP} NA P{UAS-sSpi.CS.GFP} NA heat-shock construct P{hs-spi.m} NA characterization construct P{Bam-8} NA P{Not-3} NA P{sev-gil::spi.ASA} NA P{sev-gil::spi.SAS} NA P{Sev-sSpi} NA P{UAS-gil::spi.A3S4A} NA P{UAS-gil::spi.A4S6A} NA P{UAS-gil::spi.AS} NA P{UAS-gil::spi.S3A4S} NA P{UAS-gil::spi.S4A6S} NA P{UAS-gil::spi.SA} NA P{UAS-SpiEC::KrnIC} NA P{UAS-T:GFP.SpiEC::KrnIC} NA P{UAS-vn.A3S4A} NA P{UAS-vn.A3S} NA P{UAS-vn.A4S} NA P{UAS-vn.S3A4S} NA P{UAS-vn.S3A} NA P{UAS-vn.S4A} NA P{UAS-vn-spi.EGF} NA P{VDRC.spi.U} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH0523SH0523 No P{lacW}spiIDB7 No P{lacW}spik05808 No P{lacW}spis3547 No P{MTW}spi133a No P{PZ}spi00233 No P{PZ}spi01068 No P{PZ}spi07132 No P{PZ}spi10681 No P{PZ}spiSCP2 No insertion of mobile activating element P{EP}spiEP2201 NA P{EP}spiEP2378 NA P{EP}spiEP2632 NA P{EP}spiEP3363 NA P{EPgy2}spiEY14184 NA P{GSV1}C623C623 NA P{GSV6}spiGS9657 NA P{XP}spid03659 NA P{XP}spid06955 NA P{XP}spid07490 NA miscellaneous insertions P{}spiP1 NA P{attP.w+.attP}J08 NA P{GT1}spiBG00586 NA P{Pry-un}spiGR883 NA P{RS3}spiCB-5296-3 NA P{RS3}spiCB-6534-3 NA P{RS3}spiCB-6667-3 NA P{RS3}spiUM-8231-3 NA P{RS3}spiUM-8232-3 NA P{ry11}spiSCP1 NA P{wHy}DG10307 NA P{wHy}spiDG04705 NA PBac{WH}spif03256 NA insertion of enhancer trap binary system P{GawB}spiNP0261 No P{GawB}spiNP0289 No P{GawB}spiNP3582 No
Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )
Terms Based on Experimental Evidence ( 16 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
behavioral response to ethanol inferred from mutant phenotype (Corl et al., 2009) border follicle cell migration inferred from mutant phenotype (McDonald et al., 2006) determination of genital disc primordium inferred from mutant phenotype (Chen et al., 2005) epithelial cell proliferation involved in Malpighian tubule morphogenesis inferred from mutant phenotype (Kerber et al., 1998) heart process inferred from mutant phenotype (Yu et al., 2010) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Dworkin and Gibson, 2006) negative regulation of apoptotic process inferred from mutant phenotype (Parker, 2006) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) oenocyte development inferred from genetic interaction with abd-A AND inferred from mutant phenotype (Brodu et al., 2002) peripheral nervous system development inferred from mutant phenotype (Sepp and Auld, 2003) positive regulation of cell proliferation inferred from mutant phenotype (Buchon et al., 2010, Jiang and Edgar, 2009) spiracle morphogenesis, open tracheal system inferred from mutant phenotype (Merabet et al., 2005) stem cell fate commitment inferred from mutant phenotype (Figeac et al., 2010) stomatogastric nervous system development inferred from mutant phenotype (Gonzalez-Gaitan and Jackle, 2000)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Lee et al., 2001, Schlesinger et al., 2004) Golgi apparatus inferred from genetic interaction with S (Lee et al., 2001)
Terms Based on Predictions or Assertions ( 16 terms )
Molecular Function
CV term Evidence References
epidermal growth factor receptor binding non-traceable author statement (van Eeden and St. Johnston, 1999) traceable author statement (Bier, 1998, Shilo, 2003) gurken receptor binding non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
Biological Process
CV term Evidence References
branched duct epithelial cell fate determination, open tracheal system traceable author statement (Zelzer and Shilo, 2000) epidermal growth factor receptor signaling pathway non-traceable author statement (Bogdan and Klaembt, 2001, Sanson, 2001) traceable author statement (Bier, 1998, Shilo, 2003) G2/M transition of mitotic cell cycle non-traceable author statement (Baker, 2001) negative regulation of apoptotic process non-traceable author statement (Hidalgo, 2002) negative regulation of compound eye retinal cell programmed cell death non-traceable author statement (Brachmann and Cagan, 2003) open tracheal system development traceable author statement (Ghabrial et al., 2003) positive regulation of epidermal growth factor receptor signaling pathway non-traceable author statement (van Eeden and St. Johnston, 1999) R8 cell-mediated photoreceptor organization non-traceable author statement (Frankfort and Mardon, 2002) salivary gland boundary specification non-traceable author statement (Bradley et al., 2001) second mitotic wave involved in compound eye morphogenesis non-traceable author statement (Baker, 2001)
Cellular Component
CV term Evidence References
endoplasmic reticulum non-traceable author statement (Shilo, 2003) extracellular region non-traceable author statement (Shilo, 2003) traceable author statement (Bier, 1998) integral to membrane non-traceable author statement (Frankfort and Mardon, 2002, Shilo, 2003) plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
spi - Arc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG1458 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG15098 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG2064 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG2199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG2918 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG3335 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG33523 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG3634 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG3714 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CG9947 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Cnx99A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - CoIV anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Crc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Egfr solid phase assay, tag visualisation by fluorescence, x-ray crystallography (Alvarado et al., 2010) spi - Gp210 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - HLH106 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Karyβ3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - KdelR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Men anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Ost48 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Pdi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosα2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosα4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosα6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosα7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosβ1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosβ3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosβ6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Prosβ7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpn2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpn6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpn8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpn9 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpt1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpt2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpt3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpt4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpt5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Rpt6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Sk2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Spt-I anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Vap-33-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - Vps28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - ZnT63C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - emb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - hts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - lace anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - mt:CoII anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - poe anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - porin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) spi - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spi allele Gene References spi1 arm (Cox et al., 2000) Egfr (Freeman, 1994, Tio et al., 1994) jing (Sonnenfeld et al., 2004) Ras85D (Wasserman and Freeman, 1998) rho (Freeman, 1994, Wasserman and Freeman, 1998, Lanoue and Jacobs, 1999) S (Freeman, 1994, Gaengel and Mlodzik, 2003) vn (Schnepp et al., 1996, Lanoue et al., 2000) W (Bergmann et al., 2002) spi2 rho (Freeman, 1994) spi3 rho (Freeman, 1994) vn (Yarnitzky et al., 1998) spi133a ph-p (Faucheux et al., 2001) spi259 S (Gaengel and Mlodzik, 2003) spiEP2378 pnr (Pena-Rangel et al., 2002) spis.Scer\UAS abd-A (Brodu et al., 2002) dpp (Kubota et al., 2000) ovo (Payre et al., 1999) rho (Lanoue and Jacobs, 1999) salm (Elstob et al., 2001) spen (Chen and Rebay, 2000) spis.sev Egfr (Freeman, 1996) sty (Casci et al., 1999) spiSC1 Egfr (Tio et al., 1994) Src42A (Zhang et al., 1999) spiSC2 Egfr (Tio et al., 1994) spiSC3 Egfr (Tio et al., 1994) spiScer\UAS.cSa S (Pickup and Banerjee, 1999, Hsiung et al., 2001) spiSCP1 edl (Yamada et al., 2003) S (Brown and Freeman, 2003) sty (Casci et al., 1999) unspecified cv-c (Brodu and Casanova, 2006) CycE (Parker, 2006) Egfr (Read et al., 2005) nej (Anderson et al., 2005) Nsf2 (Laviolette et al., 2005) pbl (Gregory et al., 2007) Ret (Read et al., 2005) ru (Wasserman et al., 2000) vg (Nagaraj et al., 1999)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61229 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005672 DroID - A comprehensive database of gene and protein interactions. • FBgn0005672 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35253 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q01083
Orthologs
OrthoDB Orthologs (29) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6K0R7V Dipteran inclusive ortholog search EOG6GTKPC Insect inclusive ortholog search EOG6B8H9V Arthropod inclusive ortholog search EOG6P2NK0 Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6K0R7V)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\spi     Drosophila simulans   Dsim\GD21745 Y   Drosophila sechellia   Dsec\GM16998 Y   Drosophila erecta   Dere\GG21620 Y   Drosophila yakuba   Dyak\GE12640 Y   Drosophila ananassae   Dana\GF15623 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA28085 Y   Drosophila persimilis   Dper\GL26227 Y   Drosophila willistoni   Dwil\GK15227 Y   Drosophila virilis   Dvir\GJ18009 Y   Drosophila mojavensis   Dmoj\GI17249 Y   Drosophila grimshawi   Dgri\GH13960     Drosophila grimshawi   Dgri\GH13498 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6B8H9V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB47654 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012079 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10657 Y Atta cephalotes Leafcutter ant  Acep\ACEP10233 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO12733 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21608 Y Linepithema humile Argentine ant  Lhum\LH14619 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB22972 Y Solenopsis invicta Red fire ant  Sinv\SINV11923 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI33281 Y Bombyx mori Silkmoth  Bmor\BGIBMGA012584 Y Pediculus humanus Human body louse  Phum\PHUM597470 Y Pediculus humanus Human body louse  Phum\PHUM408450 Y Tribolium castaneum Red flour beetle  Tcas\Krn Y
Orthologs in non-Insect Arthropods (EOG6P2NK0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_271340 Y Ixodes scapularis Deer tick  Isca\ISCW002520 Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21745 Drosophila sechellia Dsec\GM16998 Drosophila erecta Dere\GG21620 Drosophila yakuba Dyak\GE12640 Drosophila ananassae Dana\GF15623 Drosophila pseudoobscura pseudoobscura Dpse\GA28085 Drosophila persimilis Dper\GL26227 Drosophila willistoni Dwil\GK15227 Drosophila virilis Dvir\GJ18009 Drosophila mojavensis Dmoj\GI17249 Drosophila grimshawi Dgri\GH13498
Stocks & Reagents
Stocks Listed in FlyBase ( 25 )
Harvard	- P{XP}spid03659 - P{XP}spid06955 - P{XP}spid07490 - PBac{WH}spif03256
Kyoto	123551 w1118; P{RS3}spiCB-5296-3 124144 w1118; P{RS3}spiCB-6534-3 124171 w1118; P{RS3}spiCB-6667-3 124430 w1118; P{RS3}spiUM-8231-3 124431 w1118; P{RS3}spiUM-8232-3
VDRC	v103817 P{KK105786}VIE-260B v3920 w1118; P{GD1779}v3920 v3922 w1118; P{GD1779}v3922
	More stocks available...
Genomic Clones ( 2 )
	BACR27M12 BACR30H01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 200 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RH69567
Other clones	DPiM_FI04747 FI04747
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH01105 GH02463 GH07354 GH11170 GH11485 GH12321 GH18135 GH22704 GH27275 GH27340 LD04545 LD06182 LD13059 LD30627 LP07427 RE06093 RE08872 RE11869 RE16316 RE27055 RE27314 RE32351 RE43674 RE46041 RE47060 RE47995 RE55466 RE56644 RE64804 RE66488 RH40705 RH42910 RH48502 RH55242 RH57568 SD03543 SD04412 SD11147 SD11962 SD13152 SD13490 SD17357 SD18912 SD19141 SD22692 SD24151 SD26955
Other clones	12074 50956 53571 64843 77700 101235 101332 101481 105741 161032 173645 214225.30 214287.26 214469.26 CK01.64A.F2 CK01.84C.A2 CK01.91D.D8 CK01.97C.C10 CK01.118A.B12 EC01371 EC26835 EK064540 EK067025 EK075641 EK090730 EK112958 EK172923 EK173807 EK176730 EK188331 EK209746 EK210963 EK220008 EK220011 EK228361 EK243550 EK244145 EK264721 EK308301 EP09140 EP11741 EP12140 FGC027A09 RE01152 RH09080 RP004029801 RP004031058 RP004042229 RP004045208 RP004046878 RP004058430 RP004072774 RP004077313 RP004078406 RP004083714 RP004096144 RP004102602 RP004104686 RP004110316 RP004136564 RP004137819 RP004145606 RP004153296 RP004173812 RP004184024 RP004190326 RP004193934 RP004198821 RP004199373 RP004210317 RP004213026 RP004213477 RP004213697 RP004214366 RP004215409 RP004216385 RP004220023 RP004223452 RP004225809 RP004226204 RP004227835 RP004230766 RP004231869 RP004232229 RP004232929 RP004233722 RP004233919 RP004234533 RP004235131 RP004235258 RP004235603 RP004237074 RP004239171 RP004239197 RP004239240 RP004240300 RP004240623 RP004242118 RP004243913 RP004246170 RP004249866 RP004255914 RP004261069 RP004265743 RP004268105 RP004268263 RP004287133 RP004287555 RP004309192 RP004317635 RP004342079 RP004369919 RP004382830 RP004392073 RP004392747 RP004395056 RP004401644 RP004404682 RP004414597 RP004415286 RP004428530 RP004435068 RP004452880 RP004464167 RP004472459 RP004489639 RP004492731 RP004493541 RP004498901 RP004499577 RP004500003 RP004500444 RP004501203 RP004503021 RP004503654 RP004506560 RP004508756 RP004510148 RP004517966 RP004518374 RP004529461 RP004531322 RP004531400 RP004534001 RP004534010 RP004536596 RP004539604 RP004541542 RP004545273 RP004559666
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0005672 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35253
Antibody Information
	polyclonal (Rutledge et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: spi anon-WO0118547.158 (FlyBase, 1996-)
Additional comments	Source for merge of spi anon-WO0118547.158 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	spi is not required for patterning of the dorsal anterior follicular epithelium. (Lachance et al., 2009) S protein appears to traffic the spi precursor while cycling between the endoplasmic reticulum and late endosomes in S[[2]]R[+] cells. (Tsruya et al., 2007) spi protein is palimtoylated. Palmitoylation of spi promotes membrane association, increasing its activity but reducing its range. (Miura et al., 2006) spi is necessary for axon contact dependent inhibition of W dependent cell death of midline glial cells. (Bergmann et al., 2002) spi/Egfr signalling via the Ras/MAPK pathway mediates the induction of bract cell fate in the leg. (del Alamo et al., 2002) Area matching Drosophila EST AA201448. This EST forms a 856bp contig with ESTs AA438721 and AA247046. (Davies, 2001.3.15) spi interacts directly with S. (Hsiung et al., 2001) rho protein appears to be an intramembrane serine protease that directly cleaves spi protein. The putative rho active site is within the membrane bilayer and the spi cleavage site is within its transmembrane domain. (Urban et al., 2001) Two EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo. (Hummel et al., 1999) hh and spi bring about the concerted assembly of ommatidial and synaptic cartridge units, imposing the "neurocrystalline" order of the compound eye onto the post-synaptic target field. spi activity is necessary and sufficient for the differentiation of cartridge neurons in the lamina. (Huang et al., 1998) EGF domain swapping experiments of vn, spi and argos demonstrate that the EGF domain is the key determinant that gives Egfr inhibitors and activators their distinct properties. (Schnepp et al., 1998) spi is required in follicle cells for dorsal-anterior patterning of the egg. (Wasserman and Freeman, 1998) When spi is removed from the follicle cells using clonal analysis the dorsal appendages fuse. The phenotype resembles that caused by a reduction in Egfr signalling. (Wasserman and Freeman, 1998) Epistasis tests with known dorsal/ventral patterning genes suggests that CrebA encodes a transcription factor near the end of both the dpp and spi signalling cascades to translate the corresponding extracellular signals into changes in gene expression. (Andrew et al., 1997) In vivo culture of mutant discs from genotypes that are normally embryonic lethal demonstrates spi has no role in wing disc growth. (Simcox, 1997) spi acts in photoreceptor recruitment in the developing retina. (Tio and Moses, 1997) The Egfr receptor pathway is activated by localized processing of the ligand spi in the tracheal placodes. (Wappner et al., 1997) The function of spi, rho and S appears to be non-autonomous; expression of the precursor only in the midline is sufficient for patterning the ventral ectoderm. (Golembo et al., 1996) Facilitating the expression of spi, rho and S is the only sim-dependent contribution of the midline to patterning the ventral ectoderm, since the mutant sim ectodermal defects can be overcome by expression of secreted spi in the ectoderm. These results suggest a mechanism for generating a graded distribution of secreted spi, which may subsequently give rise to graded activation of Egfr in the ectoderm. (Golembo et al., 1996) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to study the zygotic lethal mutation. (Perrimon et al., 1996) Mutations of spi, the putative ligand for Egfr in longitudinal vein formation, have no effect on penetrance of ectopic crossveins in chicgdh-5/EgfrE1 heterozygotes. (Doberstein et al., 1995) trh and the spi group of genes probably act in two separate pathways that are both necessary for proper salivary duct development. (Jones et al., 1995) In spi mutants the pre-duct cells are converted to a pre-gland fate. (Jones et al., 1995) In salivary gland development the activity of fkh prevents the expression of duct-specific cells, and in preduct cells the spi group signalling pathway prevents the expression of gland specific markers. fkh is repressed by spi group signalling, which is strongest in the most ventral cells of the epidermis. (Kuo et al., 1995) The spi product triggers the Egfr signaling cascade. Graded activation of the Egfr pathway may normally give rise to a repertoire of discrete cell fates in the ventral ectoderm and graded distribution of spi may be responsible for the graded activation. The rho and S products may act as modulators of Egfr signaling. Epistatic relationships suggest that rho and S may normally facilitate processing of the spi precursor. (Schweitzer et al., 1995) spi is required for photoreceptor determination; mutations modify the phenotype caused by ectopic expression of rho in the eye. Mosaic analysis suggest spi produces a diffusible signal during ommatidial development. Other members of the spi group and Egfr also interact with rho, in a pattern that suggests a model in which rho can act as a mediator of a ligand-receptor interaction between spi and Egfr in the developing eye. (Freeman, 1994) Clonal analysis demonstrates spi is required in the founding photoreceptor cells. (Tio et al., 1994) Required for normal development of blastoderm cells just lateral to the ventral mesodermal precursors. spi+ required in the female germ line; spi- pole cells transplanted into normal embryos produce no progeny. (Lindsley and Zimm, 1992) Embryonic lethal. Denticle bands narrower than normal; first row often missing; reversal of polarity between anterior and posterior rows not seen; irregular fusion in midline of adjacent dentical bands. Keilin's organs missing or strongly reduced. Labrum, antennal-maxillary complex and labial sense organ reduced in size; right and left halves of head skeleton fused to produce pointed appearance. Tail region normal. Right and left halves of ventral ganglia of the central nervous system closer together than normal resulting in shorter commissures. The cells in the CNS that stain with anti-eve+ antibodies are closer together in spi- than in wild type. (Lindsley and Zimm, 1992) spi gene product is required for the proper development of the ventralmost cuticle and the CNS midline. (Panzer et al., 1992) spi encodes a ligand that functionally interacts with the products of rho and possibly Egfr. (Rutledge et al., 1992) rho, pnt, S and spi all function in the formation of the same chordotonal organs. (Bier et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057561 NM_134291 NM_134292 NM_134293 NM_001032110 RefSeq (Proteins) • NP_476909 NP_599118 NP_599119 NP_599120 NP_001027281 DDBJ / EMBL / GenBank • AA201448 AAA28894 AAF53831 AAF53831.1 AAF53831.2 AAG22442 AAG22442.1 AAG22442.2 AAL90431 AAN11050 AAN11050.1 AAN11050.2 AAN11051 AAN11051.1 AAN11051.2 AAN11052 AAN11052.1 AAN11052.2 AAN11053 AAN11053.1 AAN11053.2 AAZ66477 AC005128 AQ073391 AQ073742 AQ073946 AQ254855 AX094034 AY089693 BI604260 CL943613 CZ473487 CZ473488 CZ475768 CZ485801 G00577 M95199 Entrez Gene - A searchable database of RefSeq genes. • 35253 UniProtKB/Swiss-Prot • Q01083 UniProtKB/TrEMBL • A4V0W1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Epidermal growth factor-like domain (IPR000742) EGF-like, conserved site (IPR013032) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH69567
Linkouts
	BioGRID - A database of protein and genetic interactions • 61229 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0005672 DroID - A comprehensive database of gene and protein interactions. • FBgn0005672 DRSC - Results from RNAi screens. • FBgn0005672 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005672 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10334-RF FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0005672 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35253 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /torstoll/spitz.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35253 modMine - Data generated by the modENCODE project. • FBgn0005672 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q01083
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	anon-WO0118547.158   CG10334 (Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001, Laviolette et al., 2005, Molnar et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1) CT29014 (Hynes and Zhao, 2000) EP(2)2378 (Pena-Rangel et al., 2002) l(2)37Fa   l(2)01068 (Beaton, 1999.12.12, ) l(2)s3547 (Perrimon et al., 1996, ) spi (Dworkin and Gibson., 2006, Kim et al., 2007, McDonald et al., 2006, Liu et al., 2006, Laviolette et al., 2005, Brodu and Casanova, 2006, Dworkin and Gibson, 2006, Mirkovic and Mlodzik, 2006, Stathopoulos and Levine, 2005, Butchar et al., 2012, Estes et al., 2008, Galindo et al., 2005, Miura et al., 2006, Mirkovic et al., 2011, Sinenko et al., 2011, Li-Kroeger et al., 2008, Maeda et al., 2007, Molnar et al., 2006, Zhao et al., 2007, Zhang et al., 2011, Montrasio et al., 2007, Hwang and Rulifson, 2011, Peng et al., 2012, Escudero et al., 2007, Nishimura et al., 2007, Maybeck and Röper, 2009, Huh et al., 2009, Schlesinger et al., 2004, Corl et al., 2009, Dworkin et al., 2009, Yan et al., 2009, Lachance et al., 2009, Brodu et al., 2004, Fetting et al., 2009, Oishi et al., 2006, Oishi et al., 2009, Wheeler et al., 2009, Magalhaes et al., 2007, Salzer et al., 2010, Kitadate and Kobayashi, 2010, Yu et al., 2010, Lee et al., 1999, Chang et al., 2001, Liu et al., 2010, Jiang and Edgar, 2009, Gutzwiller et al., 2010, Jiang et al., 2011, Dworkin et al., 2011, Neumüller et al., 2011, Park et al., 2011, Roy et al., 2011, Reid et al., 2012, Karim and Moore, 2011, Murillo-Maldonado et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Yogev et al., 2010, Sinenko et al., 2011, Chang et al., 2003, Maeng et al., 2012) Spi (Frasch, 1999, Friedman and Perrimon, 2006, Alvarado et al., 2006, Gebelein et al., 2008, Yamashita et al., 2005, Philippakis et al., 2006, Yogev et al., 2010, Tsruya et al., 2007, Yogev et al., 2008, Monserrate and Baker Brachmann, 2007, Miura et al., 2008, Nie et al., 2009, Grigorian et al., 2011, Ren et al., 2010, Yoshiyama-Yanagawa et al., 2011, Sun et al., 2012, Melen et al., 2005) Spitz (Wang et al., 2000, Reeves et al., 2006, Klein et al., 2008) s-spi (Montell, 2006) sSpi (Majumdar et al., 2010)
Name Synonym	spitz (Guichard et al., 1997, Kim et al., 2007, Schlesinger and Shilo, 2005, Terry et al., 2006, Gregory et al., 2007, Dworkin and Gibson, 2006, Urban, 2006, Fuller and Spradling, 2007, Estes et al., 2008, Galindo et al., 2005, Brown et al., 2006, Maeda et al., 2007, Gonzalez-Gaitan and Jackle, 2000, Philippakis et al., 2006, Montrasio et al., 2007, Zhou et al., 2008, Figeac et al., 2010, Sandmann et al., 2006, Maybeck and Röper, 2009, Mao and Freeman, 2009, Hurlbut et al., 2009, Ninov et al., 2009, Zhang et al., 2009, Salzer et al., 2010, Ashton-Beaucage et al., 2010, Lee et al., 1999, Chang et al., 2001, Liu et al., 2010, Jiang and Edgar, 2009, Buchon et al., 2010, Gutzwiller et al., 2010, Jiang et al., 2011, Park et al., 2011, Sinenko et al., 2011, Maeng et al., 2012) Spitz (Reeves and Shvartsman, 2007, Gilboa and Lehmann, 2007, Lawrence, 2006, Rodrigues et al., 2005, Parker, 2006, McDonald et al., 2006, Yogev et al., 2007, Baker, 2007, Gilboa and Lehmann, 2006, Ono et al., 2006, Jekely et al., 2005, Anonymous, 2007, Voas and Rebay, 2004, Montell, 2006, Wendler et al., 2006, Li-Kroeger et al., 2008, Yogev et al., 2008, Steinhauer and Treisman, 2008, Gebelein et al., 2008, Yamashita et al., 2005, Strigini, 2005, Vinos and Freeman, 2000, Strisovsky et al., 2009, Wang and Hartenstein, 2006, Zettl et al., 2011, Brown et al., 2007, Firth and Baker, 2007, Iyadurai et al., 2008, Nie et al., 2009, O'Keefe et al., 2009, Oishi et al., 2006, Alvarado et al., 2010, Kitadate and Kobayashi, 2010, Yu et al., 2010, Hornstein et al., 2003, Ren et al., 2010, Yasugi et al., 2010, Majumdar et al., 2010, Reid et al., 2012, Hardy and Resh, 2012, Murillo-Maldonado et al., 2011)
Secondary FlyBase IDs
	FBgn0002048 FBgn0003474 FBgn0010472 FBgn0010690 FBgn0062206
References ( 524 )
Generate a list of	All references relating to this gene ( 524 )
List References by type	Research paper  ( 293 ) Supplementary material  ( 11 ) Review  ( 113 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 17 ) Abstract  ( 76 ) FlyBase analysis  ( 3 ) Stock list  ( 2 ) Patent  ( 1 ) Book  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Conference report  ( 1 )
Recent research papers ( 23 )
	Rahn et al., 2013, Learn. Mem. 20(4): 194--200 EGFR signaling in the brain is necessary for olfactory learning in Drosophila larvae. [FBrf0221122] Butchar et al., 2012, Genetics 191(4): 1213--1226 New negative feedback regulators of egfr signaling in Drosophila. [FBrf0219175] Foronda et al., 2012, PLoS Genet. 8(8): e1002874 Drosophila Hox and Sex-Determination Genes Control Segment Elimination through EGFR and extramacrochetae Activity. [FBrf0219212] Hardy and Resh, 2012, J. Biol. Chem. 287(51): 42881--42889 Identification of N-terminal Residues of Sonic Hedgehog Important for Palmitoylation by Hedgehog Acyltransferase. [FBrf0220295] Maeng et al., 2012, Mol. Cells 33(5): 457--463 The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development. [FBrf0218378] Parrott et al., 2012, PLoS ONE 7(5): e36460 Control of germline stem cell division frequency - a novel, developmentally regulated role for epidermal growth factor signaling. [FBrf0218312] Peng et al., 2012, Dev. Cell 23(3): 507--518 Planar Polarized Protrusions Break the Symmetry of EGFR Signaling during Drosophila Bract Cell Fate Induction. [FBrf0219458] Reid et al., 2012, Front. Cell. Infect. Microbiol. 2: 24 Identification of genetic modifiers of CagA-induced epithelial disruption in Drosophila. [FBrf0219288] Sun et al., 2012, G3 (Bethesda) 2(5): 551--553 Simplified insertion of transgenes onto balancer chromosomes via Recombinase-Mediated Cassette Exchange. [FBrf0218264] Dworkin et al., 2011, Genetics 187(4): 1171--1184 The effects of weak genetic perturbations on the transcriptome of the wing imaginal disc and its association with wing shape in Drosophila melanogaster. [FBrf0214376] Grigorian et al., 2011, Dev. Biol. 353(1): 105--118 The convergence of Notch and MAPK signaling specifies the blood progenitor fate in the Drosophila mesoderm. [FBrf0213410] Hwang and Rulifson, 2011, Development 138(14): 2883--2893 Serial specification of diverse neuroblast identities from a neurogenic placode by Notch and Egfr signaling. [FBrf0214015] Jiang et al., 2011, Cell Stem Cell 8(1): 84--95 EGFR/Ras/MAPK Signaling Mediates Adult Midgut Epithelial Homeostasis and Regeneration in Drosophila. [FBrf0212694] Karim and Moore, 2011, J. Neurosci. 31(47): 17017--17027 Convergent local identity and topographic projection of sensory neurons. [FBrf0216770] Murillo-Maldonado et al., 2011, PLoS ONE 6(11): e28067 Insulin Receptor-Mediated Signaling via Phospholipase C-γ Regulates Growth and Differentiation in Drosophila. [FBrf0216849] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Park et al., 2011, PLoS Genet. 7(8): e1002241 Specification of Drosophila corpora cardiaca neuroendocrine cells from mesoderm is regulated by Notch signaling. [FBrf0215231] Roy et al., 2011, Science 332(6027): 354--358 Specificity of Drosophila cytonemes for distinct signaling pathways. [FBrf0213449] Sinenko et al., 2011, EMBO Rep. 13(1): 83--89 Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila. [FBrf0217071] Xu et al., 2011, Dev. Biol. 354(1): 31--43 EGFR, Wingless and JAK/STAT signaling cooperatively maintain Drosophila intestinal stem cells. [FBrf0213634] Yoshiyama-Yanagawa et al., 2011, J. Biol. Chem. 286(29): 25756--25762 The Conserved Rieske Oxygenase DAF-36/Neverland Is a Novel Cholesterol-metabolizing Enzyme. [FBrf0214362] Zettl et al., 2011, Cell 145(1): 79--91 Rhomboid Family Pseudoproteases Use the ER Quality Control Machinery to Regulate Intercellular Signaling. [FBrf0213387] Zhang et al., 2011, PLoS ONE 6(10): e26197 Mastermind Mutations Generate a Unique Constellation of Midline Cells within the Drosophila CNS. [FBrf0216654] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...