chromatin component - BTB/POZ domain protein- responsible for establishment of transcriptional quiescence in germ cells - a key regulator of centrosome separation required for proper primordial germ cell development
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.53
None of the polypeptides share 100% sequence identity.
569 (aa); 65 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gcl using the Feature Mapper tool.
gcl expression at embryonic stages 1-4 is localized to the posterior pole of the embryo.
gcl transcripts are expressed during oogenesis and throughout embryonic development. Transcripts are first detected in nurse cells at oogenesis stage S8 and are abundant in nurse cells at stage S10. Transcripts first enter the oocyte in stage S11 and later become posteriorly localized. The posterior localization is maintained through the syncytial nuclear cleavage stage of embryogenesis and the gcl transcripts are subsequently incorporated into pole cells as they form. The staining is punctate and perinuclear suggesting that transcripts may be associated with polar granules. Later in embryogenesis, a dynamic pattern of gcl expression is observed in a number of tissues including the foregut, hindgut, muscle, and a subset of the cells of the CNS.
At the pole bud stage, gcl protein appears to be associated with the plasma membrane in addition to diffuse cytopasmic staining and staining of pole bud nuclei. After pole bud division, gcl protein concentrates at the nuclear membrane with a low level of diffuse staining within the nucleus.
GBrowse - Visual display of RNA-Seq signalsView Dmel\gcl in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
gcl acts to repress transcription during the establishment of the germ cell lineage.
Localisation of gcl to the nuclear envelope is crucial for promoting pole cell formation, but not pole bud formation. Overall orientation not stated: gcl+ cmp44E-
gcl activity is required only for the establishment of the germ cell lineage: proper pole bud formation, pole cell formation and pole cell survival.
gcl is required for the specification of pole cells but not posterior somatic cells. Mothers with reduced levels of gcl give rise to progeny that lack pole cells, ectopic expression causes a transient increase in pole cells and ectopic localisation in the anterior pole causes somatic cells to adopt characteristics of pole cells.
Elevation of gcl levels increases the number of nuclei that initiate pole cell formation, these pole cells undergo fewer divisions or die before or during cellular blastoderm.