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Gene Dmel\DNApol-α73

General Information
Symbol	Dmel\DNApol-α73	Species	D. melanogaster
Name	DNA polymerase α 73kD	Annotation symbol	CG5923
Feature type	protein_coding_gene	FlyBase ID	FBgn0005696
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	Polα, pol α
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	97E10-97E10	Sequence location	3R:23,064,903..23,066,950 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene DNA polymerase α 73kD is referred to in FlyBase by the symbol Dmel\DNApol-α73 (CG5923, FBgn0005696). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: DNA-directed DNA polymerase activity. Based on sequence similarity, it is predicted to be involved in the biological process: DNA-dependent DNA replication. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA polymerase alpha, subunit B; DNA polymerase alpha/epsilon, subunit B. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1628372_a_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of DNApol-alpha73. Gene sequence location is 3R:23064903..23066950.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN21474 HFA16641
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 97E10-97E10   Limits computationally determined from genome sequence between P{PZ}sda03884 and P{PZ}btzrL203
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\DNApol-α73 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) DNApol-α73-RC FBtr0114543  NM_170327   2048   609
Additional Transcript Data & Comments
Reported size (kB)	2.5 (northern blot) (Cotterill et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein DNApol-α73-PC   FBpp0113035   67.0   609   5.17     NP_733206   AAF56680
Additional Polypeptide Data & Comments
Reported size (kDa)	653 (aa); 73 (kD observed) (Cotterill et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA polymerase alpha/epsilon, subunit B (IPR007185) DNA polymerase alpha, subunit B (IPR016722)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696768   AE014297 AAF56680   AI455812   AI515782   AW941312   AY060943 AAL28491   BG635903   BI167591   BI235665   BI243696   CK132747   CO293163   CO340166   EC212544   EC258134   EV575329   EV582332   EV582458   GH941575   GH942665   GH945345   GH945720   GH957787   GH959935   GH961421   GH961739   GH963427   GH972064   GH977924   GH978053   GH982119   S43476   S43478
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VB62
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000160853 3R:23,064,789..23,064,805 bound_moiety=Dref-XP evidence=experimental (Takahashi et al., 1996, Bergman et al., 2004) protein binding site FBsf0000160854 3R:23,064,863..23,064,884 bound_moiety=Dref-XP evidence=experimental (Takahashi et al., 1996, Bergman et al., 2004) protein binding site FBsf0000160855 3R:23,064,812..23,064,833 bound_moiety=Dref-XP evidence=experimental (Takahashi et al., 1996, Bergman et al., 2004)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference fertilized egg stage   male pronucleus   • chromatin (Loppin et al., 2001) Comment:interphase female pronucleus   • chromatin (Loppin et al., 2001) Comment:interphase
Additional Descriptive Data
	mus209 and DNApol-alpha73 proteins are detected associated with the nuclear chromatin of interphase apposed male and female pro-nuclei in newly fertilized eggs in preparation for the completion of DNA replication prior to the first zygotic division. (Loppin et al., 2001)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0005696    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0005696 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0005696 expression level Developmental Stage   Expression Level embryo 00-02hr    5353 embryo 02-04hr    1950 embryo 04-06hr    4082 embryo 06-08hr    2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day    2420 adult female 30day    2666 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5353) embryo 02-04hr  (1950) embryo 04-06hr  (4082) embryo 06-08hr  (2068) embryo 08-10hr  (1091) embryo 10-12hr  (534) embryo 12-14hr  (228) embryo 14-16hr  (449) embryo 16-18hr  (317) embryo 18-20hr  (191) embryo 20-22hr  (113) embryo 22-24hr    95 larva L1  (646) larva L2  (487) larva L3 12hr old  (304) larva L3 puffstage 1-2  (250) larva L3 puffstage 3-6  (259) larva L3 puffstage 7-9  (271) white prepupae new  (235) white prepupae 12hr  (507) white prepupae 24hr  (308) pupae 2d postWPP  (121) pupae 3d postWPP    94 pupae 4d postWPP  (214) adult male 01day  (115) adult male 05day  (135) adult male 30day  (168) adult female 01day  (1376) adult female 05day  (2420) adult female 30day  (2666) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5353) embryo 02-04hr  (1950) embryo 04-06hr  (4082) embryo 06-08hr  (2068) embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day  (2420) adult female 30day  (2666) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5353 embryo 02-04hr    1950 embryo 04-06hr    4082 embryo 06-08hr    2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day    2420 adult female 30day    2666 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5353 embryo 02-04hr    1950 embryo 04-06hr    4082 embryo 06-08hr    2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day    2420 adult female 30day    2666 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0005696 expression level Developmental Stage   Expression Level embryo 00-02hr    5353 embryo 02-04hr    1950 embryo 04-06hr    4082 embryo 06-08hr    2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day    2420 adult female 30day    2666 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5353) embryo 02-04hr  (1950) embryo 04-06hr  (4082) embryo 06-08hr  (2068) embryo 08-10hr  (1091) embryo 10-12hr  (534) embryo 12-14hr  (228) embryo 14-16hr  (449) embryo 16-18hr  (317) embryo 18-20hr  (191) embryo 20-22hr  113 embryo 22-24hr    95 larva L1  (646) larva L2  (487) larva L3 12hr old  (304) larva L3 puffstage 1-2  (250) larva L3 puffstage 3-6  (259) larva L3 puffstage 7-9  (271) white prepupae new  (235) white prepupae 12hr  (507) white prepupae 24hr  (308) pupae 2d postWPP  121 pupae 3d postWPP    94 pupae 4d postWPP  (214) adult male 01day  115 adult male 05day  135 adult male 30day  (168) adult female 01day  (1376) adult female 05day  (2420) adult female 30day  (2666) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5353) embryo 02-04hr  1950 embryo 04-06hr  (4082) embryo 06-08hr  2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day  2420 adult female 30day  (2666) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5353 embryo 02-04hr    1950 embryo 04-06hr    4082 embryo 06-08hr    2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day    2420 adult female 30day    2666 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5353 embryo 02-04hr    1950 embryo 04-06hr    4082 embryo 06-08hr    2068 embryo 08-10hr    1091 embryo 10-12hr    534 embryo 12-14hr    228 embryo 14-16hr    449 embryo 16-18hr    317 embryo 18-20hr    191 embryo 20-22hr    113 embryo 22-24hr    95 larva L1    646 larva L2    487 larva L3 12hr old    304 larva L3 puffstage 1-2    250 larva L3 puffstage 3-6    259 larva L3 puffstage 7-9    271 white prepupae new    235 white prepupae 12hr    507 white prepupae 24hr    308 pupae 2d postWPP    121 pupae 3d postWPP    94 pupae 4d postWPP    214 adult male 01day    115 adult male 05day    135 adult male 30day    168 adult female 01day    1376 adult female 05day    2420 adult female 30day    2666 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0005696    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0005696 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0005696 expression level Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0005696 expression level Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    76.25 Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    56.1 Larval Trachea    33.425 Larval Carcass    40 Adult Head no informative data Adult Eye    8.4 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    6.4 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    252.9 Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    8.4   NA  Brain    no informative data   76.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   no informative data  Midgut    no informative data   no informative data  Hindgut    no informative data   no informative data  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   56.1  Salivary Gland    no informative data   NA  Heart    6.4   33.425  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    252.9   NA  Testis    no informative data   NA  Male Accessory Gland    no informative data   40  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005696 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5923-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of DNApol-α73 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of DNApol-α73 Class Mutagen Stocks Known lesion DNApol-α73GD4243 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes DNApol-α73KK101423 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6207 (Ryder, 2004.4.26) Df(3R)Exel6208 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR007185 (FlyBase Curators et al., 2004-) DNA-directed DNA polymerase activity inferred from sequence or structural similarity (Cotterill et al., 1992) inferred from sequence or structural similarity with UniProtKB:Q14181 (FlyBase, 1992-)
Biological Process
CV term Evidence References
DNA-dependent DNA replication inferred from sequence or structural similarity (Cotterill et al., 1992) DNA replication inferred from electronic annotation with InterPro:IPR007185 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
alpha DNA polymerase:primase complex inferred from sequence or structural similarity (Cotterill et al., 1992) inferred from sequence or structural similarity with UniProtKB:Q14181 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions DNApol-α73 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68166 DroID - A comprehensive database of gene and protein interactions. • FBgn0005696 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43278
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16278 Dere\GG11537 Dgri\GH16851 Dmoj\GI10451 Dper\GL24481 Dpse\GA19232 Dsec\GM10379 Dsim\GD15188 Dvir\GJ10668 Dwil\GK22573 Dyak\GE23725
Curated drosophilid orthologs	Dpse\GA19232   Dsim\DNApol-α73 (Schlenke, 2001.3.12)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007692-PA Aedes aegypti (mosquito) AGAP004392-PA Anopheles gambiae (mosquito) XP_001120826.1 Apis mellifera (honey bee) At1g67630.1 Arabidopsis thaliana (wall cress) 12741 Batrachochytrium dendrobatidis (chytrid fungus) 127341 Branchiostoma floridae (lancelet) XP_001893716.1 Brugia malayi (roundworm nematode) BGIBMGA005158-PA Bombyx mori (silk worm) ENSBTAP00000015221 Bos taurus (bovine) CAL0004305 Candida albicans (a form of yeast) CN06234 Caenorhabditis brenneri (small nematode) CBP13228 Caenorhabditis briggsae (nematode) CE28840 Caenorhabditis elegans (worm) ENSCAFP00000020160 Canis familiaris (dog) CAGL0F04323g Candida glabrata (haploid yeast) ENSCINP00000006438 Ciona intestinalis (sea squirt) JA11963 Caenorhabditis japonica (nematode) CMF127C Cyanidioschyzon merolae (red alga) CPIJ001713 Culex pipens (mosquito) ENSCPOP00000001676 Cavia porcellus (guinea pig) 146295 Chlamidomonas reinhardtii (green alga) RP05604 Caenorhabditis remanei (nematode) 95153 Capitella spl (polychaete worm) DDB0232277 Dictyostelium discoideum (amoeba) DEHA2F25806g Debaryomyces Hansenii (yeast) 321802 Daphnia pulex (daphnia) ENSDARP00000002618 Danio rerio (zebra fish) ENSECAP00000021873 Equus caballus (horse) ENSGACP00000023338 Gymnopilus aculeatus (mushroom) 185050 Hypsipyla robusta (cedar shoot borer) ENSP00000265465 Homo sapiens (human) KLLA0B12309g Kluyveromyces lactis (yeast) 110777 Lottia gigantea (owl limpet) 29731 Monocosta brevicollis (choanoflagellate) ENSMODP00000012318 Monodelphis domestica (gray opossum) ENSMMUP00000029011 Macaca mulatta (rhesus monkey) ENSMUSP00000025752 Mus musculus (house mouse) 166478 Nematostella vectensis (starlet anemone) XP_001601850.1 Nasonia vitripennis (wasp) ENSOANP00000022420 Ornithorynchus anatinus (platypus) ENSORLP00000003629 Oryzias latipes (japanese killifish) LOC_Os12g13950.1 Oryza sativa (asian rice) 12847 Ostreococcus tauri (green alga) PHUM003053-PA Pediculus humanus (human body louse) 138265 Physcomitrella patens (moss) ENSPPYP00000003554 Pongo pygmaeus (orangutan) 73133 Phytophthora ramorum (sudden oak death pathogen) 230224 Populus trichocarpa (black cottonwood) ENSPTRP00000006673 Pan troglodytes (chimpanzee) ENSRNOP00000028406 Rattus norvegicus (norway rat) 5031247 Sorghum bicolor (sorghum) XP_780208.2 Strongylocentrotus purpuratus (purple sea urchin) 57222 Trichoplax adhaerens (metazoa) XP_001807778.1 Tribolium castaneum (red flour beetle) ENSTNIP00000019899 Tetraodon nigroviridis (green spotted pufferfish) 3787 Thalassiosira pseudonana (marine diatom) ENSTRUP00000037922 Takifugu rubripes (pufferfish Takifugu) 3725.m00016 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000050316 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15441 LH23065 ISCW002323 FBgn0240883 FBgn0224550 FBgn0197946 FBgn0186858 FBgn0165329 JGI_V11_321802 FBgn0079229 FBgn0162070 FBgn0133215 FBgn0124322 FBgn0103834 FBgn0093300 CPIJ001712 CPIJ001713 BGIBMGA005158 ACYPI008858 GB12486 AGAP004392 AAEL009905 AAEL007692 AAEL001823 PB24865 PHUM003740 GLEAN_05585
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v108579 P{KK101423}VIE-260B v11232 w1118; P{GD4243}v11232/CyO
Genomic Clones ( 1 )
	BACR02B05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM08365
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD35513 LD41453 RE31570 RE41412 SD13421
Other clones	73162 214447.22 Dmel_dig_002238_0656_3439 Dmel_dig_007551_0626_3083 Dmel_dig_009641_0652_2751 EK201949 EP10133 RE08009 RP004050148 RP004225071 RP004227750 RP004231364 RP004259436 RP004264866 RP004265381 RP004266372 RP004403393 RP004514663 RP004532365 RP004535660 RP004551439
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0005696 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43278
Antibody Information
	polyclonal (Cotterill et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Analysis of DNApol-α73-Ecol\CAT deletion constructs shows that full promoter activity is located within the region from -285 to +129bp of DNApol-α73. Three DREs (DNA-replication related elements) are located within this region, and DNA footprinting analysis shows that Dref can bind to all three sites. Mutation of any one of the DRE sites causes extensive reductions in promoter activity and Dref binding to the promoter fragment. (Takahashi et al., 1996) A monoclonal antibody to the 72kD protein stains interphase nuclei, but not metaphase chromosomes. The protein is present in the embryo as a maternal product. (Kuroda and Ueda, 1995) Subunits of DNA polymerase α and δ are purified from embryos and characterised. (Mitsis et al., 1995) Expression pattern and subcellular distribution of the protein during development is studied using antibodies. (Yamaguchi et al., 1995) The properties of the DNA polymerase α enzyme have been studied. (Cai et al., 1993) The gene for the 73kD subunit of the DNA polymerase α primase has been cloned and nucleotide sequence motifs constituting control regions have been analysed. (Cotterill et al., 1992) 25bp DNA oligomer containing m4T is used in a gel extension assay to measure the efficiency of incorporation of dATP and dGTP opposite m4T using a DNA polymerase α-primase complex. The DNA polymerase α shows a clear preference for pairing with dGTP. (Dosanjh et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_170327 RefSeq (Proteins) • NP_733206 DDBJ / EMBL / GenBank • AA696768 AAF56680 AAL28491 AI515782 AW941312 AY060943 BI167591 S43476 S43478 Entrez Gene - A searchable database of RefSeq genes. • 43278 UniProtKB/TrEMBL • Q9VB62
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA polymerase alpha/epsilon, subunit B (IPR007185) DNA polymerase alpha, subunit B (IPR016722)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68166 DroID - A comprehensive database of gene and protein interactions. • FBgn0005696 DRSC - Results from RNAi screens. • FBgn0005696 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005696 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5923-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0005696 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43278 InParanoid A subset of ortholog calls from InParanoid. • AAEL007692-PA Aedes aegypti (mosquito) AGAP004392-PA Anopheles gambiae (mosquito) XP_001120826.1 Apis mellifera (honey bee) At1g67630.1 Arabidopsis thaliana (wall cress) 12741 Batrachochytrium dendrobatidis (chytrid fungus) 127341 Branchiostoma floridae (lancelet) XP_001893716.1 Brugia malayi (roundworm nematode) BGIBMGA005158-PA Bombyx mori (silk worm) ENSBTAP00000015221 Bos taurus (bovine) CAL0004305 Candida albicans (a form of yeast) CN06234 Caenorhabditis brenneri (small nematode) CBP13228 Caenorhabditis briggsae (nematode) CE28840 Caenorhabditis elegans (worm) ENSCAFP00000020160 Canis familiaris (dog) CAGL0F04323g Candida glabrata (haploid yeast) ENSCINP00000006438 Ciona intestinalis (sea squirt) JA11963 Caenorhabditis japonica (nematode) CMF127C Cyanidioschyzon merolae (red alga) CPIJ001713 Culex pipens (mosquito) ENSCPOP00000001676 Cavia porcellus (guinea pig) 146295 Chlamidomonas reinhardtii (green alga) RP05604 Caenorhabditis remanei (nematode) 95153 Capitella spl (polychaete worm) DDB0232277 Dictyostelium discoideum (amoeba) DEHA2F25806g Debaryomyces Hansenii (yeast) 321802 Daphnia pulex (daphnia) ENSDARP00000002618 Danio rerio (zebra fish) ENSECAP00000021873 Equus caballus (horse) ENSGACP00000023338 Gymnopilus aculeatus (mushroom) 185050 Hypsipyla robusta (cedar shoot borer) ENSP00000265465 Homo sapiens (human) KLLA0B12309g Kluyveromyces lactis (yeast) 110777 Lottia gigantea (owl limpet) 29731 Monocosta brevicollis (choanoflagellate) ENSMODP00000012318 Monodelphis domestica (gray opossum) ENSMMUP00000029011 Macaca mulatta (rhesus monkey) ENSMUSP00000025752 Mus musculus (house mouse) 166478 Nematostella vectensis (starlet anemone) XP_001601850.1 Nasonia vitripennis (wasp) ENSOANP00000022420 Ornithorynchus anatinus (platypus) ENSORLP00000003629 Oryzias latipes (japanese killifish) LOC_Os12g13950.1 Oryza sativa (asian rice) 12847 Ostreococcus tauri (green alga) PHUM003053-PA Pediculus humanus (human body louse) 138265 Physcomitrella patens (moss) ENSPPYP00000003554 Pongo pygmaeus (orangutan) 73133 Phytophthora ramorum (sudden oak death pathogen) 230224 Populus trichocarpa (black cottonwood) ENSPTRP00000006673 Pan troglodytes (chimpanzee) ENSRNOP00000028406 Rattus norvegicus (norway rat) 5031247 Sorghum bicolor (sorghum) XP_780208.2 Strongylocentrotus purpuratus (purple sea urchin) 57222 Trichoplax adhaerens (metazoa) XP_001807778.1 Tribolium castaneum (red flour beetle) ENSTNIP00000019899 Tetraodon nigroviridis (green spotted pufferfish) 3787 Thalassiosira pseudonana (marine diatom) ENSTRUP00000037922 Takifugu rubripes (pufferfish Takifugu) 3725.m00016 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000050316 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43278 modMine - Data generated by the modENCODE project. • FBgn0005696 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15441 LH23065 ISCW002323 FBgn0240883 FBgn0224550 FBgn0197946 FBgn0186858 FBgn0165329 JGI_V11_321802 FBgn0079229 FBgn0162070 FBgn0133215 FBgn0124322 FBgn0103834 FBgn0093300 CPIJ001712 CPIJ001713 BGIBMGA005158 ACYPI008858 GB12486 AGAP004392 AAEL009905 AAEL007692 AAEL001823 PB24865 PHUM003740 GLEAN_05585 REDfly - A database of transcriptional regulatory elements. • FBgn0005696
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	CG5923 (Dimova et al., 2003, Schlenke, 2001.3.12, Keleman et al., 2009.8.5) Dmpol α (Takeuchi et al., 2004) DNA pol-73 (Foglietti et al., 2006) DNApol-alpha73 (Giot et al., 2003) DNA polα (Frolov et al., 2003) DNApolα (Cai et al., 1993) DNApol-α (Shen et al., 1992) DNApol-α72 (Kuroda and Ueda, 1995) DNApol-α73 (Cayirlioglu et al., 2003) Pol (Machida et al., 2005) POLA (Matsukage et al., 1995) pol α (Mizushina et al., 2003, Singer and Dosanjh, 1990) Polα (Fuss et al., 2001, Lehman, 1998) polα (Royzman and Orr-Weaver, 1998)
Name Synonym	B subunit (Kuroda and Ueda, 1999) DNA polymerase 73K (Hochheimer et al., 2002) DNA polymerase alpha primase 73 kda subunit (Cotterill, 1992.11.13, Cotterill, 1992.11.13) DNA-polymerase α (Leclerc et al., 1996) DNA polymerase α (Kamakaka et al., 1994, Marton et al., 1994, Mitsis et al., 1993, Matsukage et al., 1995) DNA polymerase α 73kD
Secondary FlyBase IDs
References ( 46 )
Generate a list of	All references relating to this gene ( 46 )
List References by type	Research paper  ( 29 ) Supplementary material  ( 2 ) Review  ( 3 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...