DNA polymerase α, pol α, Polα 73kDa, DNA polymerase alpha primase 73 kda subunit, Polα
Gene model reviewed during 5.46
Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
653 (aa); 73 (kD observed)
DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Subunit B binds to subunit A (By similarity).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DNApol-α73 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\DNApol-α73 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Analysis of DNApol-α73-Ecol\CAT deletion constructs shows that full promoter activity is located within the region from -285 to +129bp of DNApol-α73. Three DREs (DNA-replication related elements) are located within this region, and DNA footprinting analysis shows that Dref can bind to all three sites. Mutation of any one of the DRE sites causes extensive reductions in promoter activity and Dref binding to the promoter fragment.
A monoclonal antibody to the 72kD protein stains interphase nuclei, but not metaphase chromosomes. The protein is present in the embryo as a maternal product.
Subunits of DNA polymerase α and δ are purified from embryos and characterised.
Expression pattern and subcellular distribution of the protein during development is studied using antibodies.
The properties of the DNA polymerase α enzyme have been studied.
The gene for the 73kD subunit of the DNA polymerase α primase has been cloned and nucleotide sequence motifs constituting control regions have been analysed.
25bp DNA oligomer containing m4T is used in a gel extension assay to measure the efficiency of incorporation of dATP and dGTP opposite m4T using a DNA polymerase α-primase complex. The DNA polymerase α shows a clear preference for pairing with dGTP.