FlyBase Gene Report: Dmel\GstD5

General Information

Symbol

Dmel\GstD5

Species

D. melanogaster

Name

Glutathione S transferase D5

Annotation Symbol

CG12242

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010041

Gene Model Status

Current

Stock Availability

5 publicly available

Enzyme Name (EC)

Glutathione transferase (2.5.1.18)

Gene Summary

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). May be involved in detoxification (PubMed:22082028). (UniProt, Q9VG95)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DmGST24, DmGSTD5, Gst, GstD24

Key Links

Genomic Location

Cytogenetic map

87B8-87B8

Sequence location

3R:12,375,733..12,376,572 [+]

Recombination map

3-52

RefSeq locus

NT_033777 REGION:12375733..12376572

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables glutathione transferase activity

inferred from direct assay

(Saisawang et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables glutathione transferase activity

inferred from biological aspect of ancestor with PANTHER:PTN001679864

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in glutathione metabolic process

inferred from direct assay

(Saisawang et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in glutathione metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN001679864

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with RGD:70952

(FlyBase Curators, 2008-)

Gene Group (FlyBase)

CYTOSOLIC GLUTATHIONE S-TRANSFERASES

Protein Family (UniProt)

Belongs to the GST superfamily. Delta family. (Q9VG95)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

RX + glutathione = HX + R-S-glutathione (2.5.1.18)

Predictions / Assertions

RX + glutathione = HX + R-S-glutathione (2.5.1.18)

Summaries

Gene Group (FlyBase)

CYTOSOLIC GLUTATHIONE S-TRANSFERASES -: Glutathione transferases (glutathione S-transferases, GST) catalyze nucleophilic attack by reduced glutathione on nonpolar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. At least 14 classes of cytosolic GSTs have been identified in prokaryotes and eukaryotes: Theta, Omega, Sigma and Zeta exist in all eukaryotes. D. melanogaster has two additional classes, Delta and Epsilon. In D. mel, the class is indicated by the letter after the Gst prefix. (Adapted from FBrf0217315 and PMID:15822171).

Protein Function (UniProtKB)

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). May be involved in detoxification (PubMed:22082028).

(UniProt, Q9VG95)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\GstD5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VG95)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Mutation in sequenced strain: premature stop (nonsense mutation): full length CDS sequence can be obtained from FBrf0058802; see GstD5[1].

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

GstD5-RA

FBtr0082572

NM_080175

840

216

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

GstD5-PA

FBpp0082044

24.7

216

7.57

NP_524914

AAF54790

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

216 (aa)

(Toung et al., 1993)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9VG95)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VG95

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GstD5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

GstD5_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

GstD5 transcripts are not detected in adults.

(Toung et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\GstD5 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\GstD5

View exonic expression by tissue for Dmel\GstD5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48338/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010041

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010041

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010041

Flygut - An atlas of the Drosophila adult midgut

FBgn0010041

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of GstD5

Transgenic constructs containing regulatory region of GstD5

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)Exel6164

(Ryder, 2004.4.26)

Df(3R)Exel6165

(Ryder, 2004.4.26)

Df(3R)Exel7315

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

GstD5^GD12981

viable, with Scer\GAL4^pnr-MD237

GstD5^GD12981

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (19)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\GSTT2B

3 of 15

Yes

Hsap\GSTT2

3 of 15

Yes

Hsap\GSTT4

3 of 15

Yes

Hsap\GSTT1

2 of 15

Hsap\LOC100652871

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (18)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Gstt1

4 of 15

Yes

Mmus\Gstt2

4 of 15

Yes

Mmus\Gstt3

4 of 15

Yes

Mmus\Gstt4

4 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (23)

Rnor\Gstt1

4 of 13

Yes

Rnor\Gstt3

4 of 13

Yes

Rnor\Gstt4

4 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\LOC103690044

1 of 13

Rnor\Mars

1 of 13

Rnor\Vars

1 of 13

Xenopus tropicalis (Western clawed frog) (10)

Xtro\gstt1

3 of 12

Yes

Xtro\gstt1

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (18)

Drer\gstt1b

4 of 15

Yes

Drer\gstt2

4 of 15

Yes

3 of 15

3 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\gst-43

2 of 15

Yes

Cele\Y53G8B.1

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (17)

Atha\GSTF9

5 of 9

Yes

Atha\GSTF10

5 of 9

Yes

Atha\ATGSTF13

3 of 9

Atha\GSTF2

3 of 9

Yes

Atha\GSTF3

3 of 9

Yes

Atha\GSTF4

3 of 9

Yes

Atha\GSTF5

3 of 9

Yes

Atha\GSTF6

3 of 9

Yes

Atha\GSTF7

3 of 9

Yes

Atha\GSTF8

3 of 9

Yes

Atha\GSTF12

3 of 9

Yes

Atha\GSTF14

3 of 9

Atha\GSTF11

2 of 9

Yes

Atha\AT2G47720

1 of 9

Yes

Atha\GSTT1

1 of 9

Atha\GSTT2

1 of 9

Atha\GSTT3

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\URE2

5 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\gst1

5 of 12

Yes

Spom\gst2

5 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (39)

Gene Symbol

Score

Source

Alignment

7 of 10

7 of 10

7 of 10

7 of 10

7 of 10

7 of 10

7 of 10

7 of 10

7 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

5 of 10

5 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GSTT2B; glutathione S-transferase theta 2B

3 of 15

GSTT2; glutathione S-transferase theta 2 (gene/pseudogene)

3 of 15

600437

GSTT4; glutathione S-transferase theta 4

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

GstD5 - GstD1

experimental knowledge based

(Guruharsha et al., 2011)

GstD5 - GstD2

experimental knowledge based

(Guruharsha et al., 2011)

GstD5 - GstD4

experimental knowledge based

(Guruharsha et al., 2011)

GstD5 - GstD7

experimental knowledge based

(Guruharsha et al., 2011)

GstD5 - GstD9

experimental knowledge based

(Guruharsha et al., 2011)

RNA-protein

Physical Interaction

Assay

References

GstD5 - mbf1

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Nishioka et al., 2018)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9VG95 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0010041

MIST (protein-protein) - An integrated Molecular Interaction Database

GstD5

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

glutathione-mediated detoxification I

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-52

Cytogenetic map

87B8-87B8

Sequence location

3R:12,375,733..12,376,572 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B8-87B8

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-52

(FlyBase, 2016)

3-49.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

42842

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02534}attP40

2057

iso-1 : y¹; Gr22b^iso-1 Gr22d^iso-1 cn¹ CG33964^iso-1 bw¹ speck¹; MstProx^iso-1 GstD5^iso-1 Rh6¹

UC San Diego

14021-0231.36

iso-1 : y¹; Gr22b^iso-1 Gr22d^iso-1 cn¹ CG33964^iso-1 bw¹ sp¹; MstProx^iso-1 GstD5^iso-1 Rh6¹

VDRC

v102234

P{KK111205}VIE-260B

v35660

w¹¹¹⁸; P{GD12981}v35660

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (9)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010041

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010041

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

48338

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: GstD24 CG12242

(Misra, 2000.7.28)

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 35 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

48338

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

M97702

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF54790

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VG95

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VG95

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VG95

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010041

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48338/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010041

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

GstD5_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010041

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010041

Flygut - An atlas of the Drosophila adult midgut

FBgn0010041

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

48338

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12242

MARRVEL_MODEL - MARRVEL (model organism gene)

48338

modMine - A data warehouse for the modENCODE project

FBgn0010041

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0010041

DRSC - Results frm RNAi screens

FBgn0010041

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010041

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

glutathione-mediated detoxification I

FlyMine - An integrated database for Drosophila genomics

FBgn0010041

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

GstD5

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

CG12242

(Hosono et al., 2015, Fukuoh et al., 2014, Mosallanejad et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Lee et al., 2003, Mount, 2001.8.14)

(Alias and Clark, 2007)

DmGST24

(Zimniak and Benes, 2002.7.15, Chelvanayagam et al., 2001, Ranson et al., 2001, Toung et al., 1993)

DmGSTD5

(Wongtrakul et al., 2012, Sawicki et al., 2003, Zimniak and Benes, 2002.7.15, Chelvanayagam et al., 2001)

GST

(Arking, 1998)

GSTD5

(Coelho et al., 2015, Saisawang and Ketterman, 2014, Li et al., 2008, Low et al., 2007)

GSTd5

(Chen et al., 2022)

Gst