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General Information
Symbol
Dmel\Jhe
Species
D. melanogaster
Name
Juvenile hormone esterase
Annotation Symbol
CG8425
Feature Type
FlyBase ID
FBgn0010052
Gene Model Status
Stock Availability
Enzyme Name (EC)
Carboxylesterase (3.1.1.1)
Juvenile-hormone esterase (3.1.1.59)
Gene Summary
Juvenile hormone esterase (Jhe) encodes a juvenile-hormone esterase involved in response to ethanol, juvenile hormone catabolism and male mating behavior. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)
Also Known As

JH, JH esterase

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-77
RefSeq locus
NT_033778 REGION:16119793..16122773
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in mitochondrion
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Catalytic Activity (EC)
Experimental Evidence
A carboxylic ester + H(2)O = an alcohol + a carboxylate (3.1.1.1)
(1) Juvenile hormone I + H(2)O = juvenile hormone I acid + methanol (3.1.1.59)
(2) Juvenile hormone III + H(2)O = juvenile hormone III acid + methanol (3.1.1.59)
Predictions / Assertions
-
Summaries
Gene Snapshot
Juvenile hormone esterase (Jhe) encodes a juvenile-hormone esterase involved in response to ethanol, juvenile hormone catabolism and male mating behavior. [Date last reviewed: 2019-09-19]
Gene Group (FlyBase)
OTHER CARBOXYLESTERASES -
OTHER CARBOXYLESTERASES are carboxylesterases that do not fall into any other major carboxylesterase group.
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Jhe for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0087223
2026
579
FBtr0301751
1979
579
FBtr0340021
1967
579
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086362
64.5
579
6.30
FBpp0290965
64.5
579
6.30
FBpp0309035
64.5
579
6.30
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

579 aa isoforms: Jhe-PA, Jhe-PB, Jhe-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Jhe using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult fat body

Comment: cyclical, peak ZT21-0

RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Expressed cyclically in the adult fat body.

Jhe transcript levels are low during embryogenesis. Peaks of expression are observed at each larval instar; these coincide with juvenile hormone expression peaks. Expression levels increase soon after pupal ecdysis. Jhe transcript is detected in both male and female adults.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in mitochondrion
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Jhe in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Jhe
Transgenic constructs containing regulatory region of Jhe
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (19)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
7 of 15
Yes
Yes
6 of 15
No
No
6 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
No
4 of 15
No
No
3  
4 of 15
No
Yes
3 of 15
No
No
1  
3 of 15
No
No
3 of 15
No
No
2  
3 of 15
No
No
3 of 15
No
No
1  
3 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (33)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
No
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
Yes
6 of 15
Yes
Yes
5 of 15
No
Yes
5 of 15
No
No
5 of 15
No
No
5 of 15
No
Yes
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
Yes
4 of 15
No
No
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (37)
7 of 13
Yes
Yes
6 of 13
No
No
6 of 13
No
No
6 of 13
No
No
5 of 13
No
No
5 of 13
No
Yes
5 of 13
No
No
5 of 13
No
No
5 of 13
No
No
5 of 13
No
Yes
4 of 13
No
No
4 of 13
No
No
4 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
Yes
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
Yes
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (22)
4 of 12
Yes
No
4 of 12
Yes
No
4 of 12
Yes
No
4 of 12
Yes
No
4 of 12
Yes
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
Yes
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (21)
6 of 15
Yes
No
6 of 15
Yes
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (47)
7 of 15
Yes
Yes
7 of 15
Yes
Yes
6 of 15
No
No
6 of 15
No
No
6 of 15
No
No
6 of 15
No
Yes
6 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
5 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
3 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904GH )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502MI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Aedes aegypti
Yellow fever mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W08S8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02T7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G05S3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (36)
6 of 10
6 of 10
6 of 10
6 of 10
6 of 10
6 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
5 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
4 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-77
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    52E5-52E5
    Limits computationally determined from genome sequence between P{PZ}l(2)0507005070&P{EP}CG8414EP525 and P{EP}spinEP645&P{lacW}spink09905
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (5)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (2)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Jhe CG8425

        Source for database merge of
        Additional comments

        Annotation CG8425 split into CG30095, CG8425 (Jhe) in release 3 of the genome annotation.

        Other Comments

        Ortholog of B. mori juvenile-hormone-related gene (involved in JH biosynthesis, metabolism or signaling).

        Jhe enzyme has been purified from prepupae and characterised biochemically.

        Juvenile hormone metabolisms in two lines of adults is studied under normal conditions and after a short period of heat stress.

        Heat stress results in delay of oviposition and a decrease in juvenile hormone hydrolysis and fertility, which lasted several days after heat treatment.

        The course of changes in the activities of enzymes degrading juvenile hormone (JH) during development is studied and the possible role of the proteins in the regulation of JH titre in critical developmental periods and in reproduction are investigated.

        Flies respond to stressing effects by a decrease in the level of Juvenile hormone (JH) hydrolysis, delay in oviposition and a decrease in fertility for a few days. The decrease in level of JH hydrolysis may be responsible for the response of the reproductive system to stress.

        Females respond to heat stress by a decrease in activity of Dvir\Jhe. This decrease is effected through a decrease in protein. Dvir\Jhe metabolism in males does not change under heat stress.

        The activity of Jhe in adult D.melanogaster females decreases in response to heat stress.

        Radiometric assay showed juvenile hormone hydrolysing activity correlates inversely with juvenile hormone titre. Juvenile hormone esterase from D.melanogaster and D.simulans is sensitive to inhibition by esterase inhibitors OTFP and DFP.

        Abnormally low levels of juvenile hormone are synthesized in ap mutants.

        Stage 8 and 9 oocytes transplanted into juvenile hormone (JH) deficient males and females were found to degenerate unless a JH analogue, ZR-515, was applied to the host shortly after implantation. These results suggest that JH is required for the initiation and continuation of vitellogenic uptake and oocyte development.

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 40 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Synonyms and Secondary IDs (11)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (75)