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Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.45
Gene model reviewed during 5.46
Gene model reviewed during 5.56
1.7, 1.0 (northern blot)
370 (aa); 40 (kD predicted)
Interacts (probably via PY-motifs) with Nedd4 (via WW2 domain) (PubMed:12165468, PubMed:16531238). Interacts with Robo (PubMed:12165468).
Ubiquitinated by Nedd4; which promotes endocytosis of the comm/robo complex and comm proteasomal degradation (PubMed:12165468). Not ubiquitinated by Nedd4 (PubMed:15657595).
The cytoplasmic domain is required for function in axon guidance.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\comm using the Feature Mapper tool.
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as ventral nerve cord anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
Comment: reported as procephalon primordium
In stage 14 embryos, comm is expressed in commissural neurons including the EG neurons.
comm expression is strong and dynamic in the embryonic nervous system. At embryonic stage 13, it is detected in neurons close to the midline and in midline glial cells. At stage 14, expression is detected throughout the ventral nerve cord and it is expressed to high levels in lateral neurons. At stage 15, expression has declined drastically in the nerve cord.
comm expression is first observed during the cellular blastoderm stage in a pattern of six pair-rule stripes. Stripe 3 is initially missing but subsequently appears. By stage 6, the stripes narrow and interstripes appear forming a pattern of 14 stripes which persist until stage 10. At this time expression is seen in two longitudinal stripes located just lateral to the neurogenic region and extending the length of the segmented embryo. A segmentally repeated pattern is also observed in the emerging CNS. comm is expressed in a subset of neuroblasts and in some ganglion mother cells. By stage 12, expression is observed in the midline of the CNS and in the visceral mesoderm. Along the midline, expression becomes restricted to a subset of cells located at the anterior part of each neuromere. There is also transient expression in neurons just lateral to the midline cells including RP1 and RP3. Double labeling experiments show that these cells lie along the path that the first commissural axons take to reach the midline. From their position, they are thought to be the anterior two pairs of midline glia. comm expression ceases in stage 16.
The 1.0kb comm transcripts are less abundant than the 1.7kb transcripts and differ only in the site of polyadenylation.
comm protein is expressed in the same cells that express comm RNA. In addition, it is observed in the first commissural axons that contact comm-expressing midline glia. It is expressed along their axons and not in the neuronal cell bodies. It therefore appears that comm protein is exported from midline cells to decorate commissural axons. In addition to the commissural axons, comm protein staining is observed in vesicles within many neuronal cell bodies throughout the nerve cord. It is thought that this represents internalization of comm protein from the surface of axons following transfer of comm protein from midline glia.
GBrowse - Visual display of RNA-Seq signals
View Dmel\comm in GBrowse 23-43
3-43
3-37.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Two EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo.
The comm gene product is required for commissural growth cones to bypass a general repulsive system operating at the midline. In the absence of comm commissural growth cones that are attracted to the midline by other mechanisms are unable to cross and ultimately retract their commissural projections.
The comm gene product plays a role in the attractive signalling system, a component of the mechanism that guides growth cones towards and away from the midline. In mutant embryos the growth cones of commissural neurons do not project across the midline, but extend only on their own side of the central nervous system (CNS).
Mutant alleles show absent commissural axon pathways in the embryonic ventral nerve cord.