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Gene Dmel\dpn

General Information
Symbol	Dmel\dpn	Species	D. melanogaster
Name	deadpan	Annotation symbol	CG8704
Feature type	protein_coding_gene	FlyBase ID	FBgn0010109
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	44C
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	44C2-44C2	Sequence location	2R:4,116,476..4,119,835 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene deadpan is referred to in FlyBase by the symbol Dmel\dpn (CG8704, FBgn0010109). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription. There is experimental evidence that it is involved in the biological process: dendrite morphogenesis; adult locomotory behavior; dosage compensation; positive regulation of neuroblast proliferation; sex determination, establishment of X:A ratio; Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; larval locomotory behavior. 17 alleles are reported. The phenotype of these alleles is annotated with: macrochaeta. It has one annotated transcript and one annotated polypeptide. Protein features are: Myc-type, basic helix-loop-helix (bHLH) domain; Orange; Orange subgroup. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1639433_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of dpn. Gene sequence location is 2R:4116476..4119835.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Alleles dpn[Scer\UAS.T:Ecol\dam,T:Hsap\MYC] Transgenic Constructs P{UAS-dpn.Dam}
FB2013_02	Controlled Vocabulary Terms embryonic/larval optic lobe third instar larval stage
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44C2-44C2   Limits computationally determined from genome sequence between P{lacW}Rs1k09514&P{lacW}l(2)k03110k03110 and P{lacW}Vps25k08904&P{lacW}ptck02507
Experimentally Determined Cytological Location
Cytogenetic map Notes References 44C-44C   (determined by in situ hybridisation)   (Bier et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\dpn for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) dpn-RA FBtr0088803  NM_057575   2036   435
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Bier et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein dpn-PA   FBpp0087879   46.6   435   7.55     NP_476923   AAF59113
Additional Polypeptide Data & Comments
Reported size (kDa)	435 (aa) (Bier et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Orange subgroup (IPR018352) Orange (IPR003650) Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA433195   AE013599 AAF59113   AC008370   AY071330 AAL48952   AY863660   AY863661   AY863662   AY863663   AY863664   AY863665   AY863666   AY863667   AY863668   AY863669   AY863670   AY863671   AY863672   AY863673   AY863674   AY863675   AY863676   AY863677   AY863678   AY863679   AY863680   AY863681   AY863682   BI238511   BI241957   BI364045   CO186775   CO296499   CO325164   CO343861   EC086389   EC213070   EC253826   GH791179   GH798617   GH814810   GH823997   GH830548   GH837170   GH841388   GH844097   GH846468   GH863744   GH882834   GH888011   GH920560   GH939481   S48025 AAB24149
UniProtKB/Swiss-Prot	Q26263
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435190 2R:4,123,275..4,124,999 evidence=Reporter construct (in vivo) (Emery and Bier, 1995, Halfon, 2012.6.19) regulatory region FBsf0000435674 2R:4,119,199..4,123,278 evidence=Reporter construct (in vivo) (Emery and Bier, 1995, Halfon, 2012.6.19) regulatory region FBsf0000435736 2R:4,120,226..4,124,994 evidence=Reporter construct (in vivo) (Emery and Bier, 1995, Halfon, 2012.6.19) regulatory region FBsf0000436023 2R:4,121,967..4,124,994 evidence=Reporter construct (in vivo) (Emery and Bier, 1995, Halfon, 2012.6.19) regulatory region FBsf0000436044 2R:4,123,825..4,124,493 evidence=Reporter construct (in vivo) (Emery and Bier, 1995, Halfon, 2012.6.19) regulatory region FBsf0000436667 2R:4,119,204..4,121,972 evidence=Reporter construct (in vivo) (Emery and Bier, 1995, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral neurectoderm   (Bier et al., 1992) neuroblast   (Bier et al., 1992) embryonic/larval neuron   (Bier et al., 1992) embryonic stage & larval stage   peripheral nervous system   (Bier et al., 1992) neuroblast   (Bier et al., 1992) central nervous system   (Bier et al., 1992) embryonic cycle 13   organism | gap expression pattern   (Bier et al., 1992) organism | pair rule expression pattern   (Bier et al., 1992) larval stage   external sensory organ   (Bier et al., 1992) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi mesectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi organism | pair rule expression pattern   (Fisher et al., 2012) trunk mesoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi embryonic stage 7 -- 8   dorsal ectoderm   (Fisher et al., 2012) hindgut anlage   (Fisher et al., 2012) mesectoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage posterior endoderm   (Fisher et al., 2012) embryonic stage 9   stage 1 neuroblast   (Emery and Bier, 1995) embryonic stage 9 -- 10   stage 1 neuroblast   (Chang et al., 2000) central brain primordium   (Fisher et al., 2012) dorsal ectoderm   (Fisher et al., 2012) inclusive hindgut primordium   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 10 -- 12   sensory mother cell   (Emery and Bier, 1995) embryonic stage 10 -- 13   neuroblast of ventral nerve cord primordium   (Emery and Bier, 1995) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as head epidermis primordium embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) germline cell   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) embryonic stage 13 -- 15   sensory neuron   (Emery and Bier, 1995) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012)
Additional Descriptive Data
	No dpn transcripts are detected in dpn1 mutant embryos. (Bier et al., 1992) dpn transcripts are first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn transcripts are expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and become restricted to the neuroblasts before they delaminate. By 6 1/2-7 hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of nervous system development of the CNS and PNS as neurons begin to differentiate. dpn is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In ventral thoracic discs, expression is also observed in additional non-neuronal cells. dpn expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background. (Bier et al., 1992)
Marker for
	stage 1 neuroblast
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval neuron   (Bier et al., 1992) embryonic stage & larval stage   peripheral nervous system   (Bier et al., 1992) embryonic cycle 13   organism | pair rule expression pattern   (Bier et al., 1992) larval stage   external sensory organ   (Bier et al., 1992) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral neurectoderm   (Bier et al., 1992) neuroblast   (Bier et al., 1992) embryonic stage & larval stage   neuroblast   (Bier et al., 1992) central nervous system   (Bier et al., 1992) embryonic cycle 13   organism | gap expression pattern   (Bier et al., 1992) embryonic stage 8   procephalic neurogenic region & Pcv7 neuroblast & Pcv9 neuroblast   (Urbach et al., 2003) embryonic antennal segment & ventral neurectoderm & neuroblast   (Urbach et al., 2003) embryonic stage 11   neuroblast NB6-4 | punctate   (Bernardoni et al., 1999) third instar larval stage   neuroblast of embryonic/larval optic lobe   (Weng et al., 2012) late third instar larval stage   neuroblast   (Siegrist et al., 2010) prepupal stage   neuroblast   (Siegrist et al., 2010) Comment:Assayed at ~5hr after pupal formation. pupal stage   neuroblast   (Siegrist et al., 2010) Comment:Assayed at ~30hr after pupal formation. pharate adult stage   neuroblast   (Siegrist et al., 2010) Comment:Assayed at mid-pupal stage.
Additional Descriptive Data
	dpn protein is first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn protein is expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and is restricted to the neuroblasts before they delaminate. By 6 1/2-7hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn protein is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of CNS and PNS development as neurons begin to differentiate. dpn protein is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In leg imaginal discs, expression is also observed in additional non-neuronal cells. dpn protein expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background. (Bier et al., 1992) No dpn protein is detected in neural precursors in dpn1 mutants. (Bier et al., 1992) ! GAP19. Used as bodypart marker for : (Liu and Belote, 1995) Approximately 100 neuroblasts in the 96hr 3rd instar larvae central nervous system express dpn. At ~5hr after pupal formation, the number of cells expressing dpn remains the same as in 3rd instar larvae, but cell size and mitotic activity are reduced. Midway through pupal development, only the mushroom body neuroblasts remain. Expression is no longer present 10hr before eclosion (~96hr APF). (Siegrist et al., 2010)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Bier et al., 1992)
Expression Deduced from Reporters
Reporter: P{lacW}dpn1 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval neuron   (Bier et al., 1992) ventral neurectoderm   (Bier et al., 1992) embryonic stage & larval stage   neuroblast   (Bier et al., 1992) central nervous system   (Bier et al., 1992) peripheral nervous system   (Bier et al., 1992) embryonic cycle 13   organism | gap expression pattern   (Bier et al., 1992) organism | pair rule expression pattern   (Bier et al., 1992) larval stage   sensory neuron of imaginal disc   (Bier et al., 1992) external sensory organ   (Bier et al., 1992) neuron of embryonic/larval nervous system   (Bier et al., 1992) neuroblast   (Bier et al., 1992)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    203.65 Larval Midgut    1.3 Larval Hindgut    7.8 Larval Malpighian Tubules    5 Larval Fat Body    2.5 Larval Salivary Gland    11 Larval Trachea    8.6 Larval Carcass    8.475 Adult Head    4 Adult Eye    0.4 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    13.9 Adult Crop    3.2 Adult Midgut    3.7 Adult Hindgut    2.3 Adult Malpighian Tubules    2.6 Adult Fat Body    14 Adult Salivary Gland    10.2 Adult Heart    1.45 Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.6 Adult Ovary    6.7 Adult Testis    2.6 Adult Male Accessory Gland    0.7 Adult Carcass    2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    4   NA  Eye    0.4   NA  Brain    9   203.65  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    3.2   1.3  Midgut    3.7   7.8  Hindgut    2.3   5  Malpighian Tubules    2.6   2.5  Fat Body    no informative data   no informative data  Salivary Gland    no informative data   NA  Heart    1.45   8.6  Trachea    NA   NA  VirginFemale Spermatheca    5.4   NA  InseminatedFemale Spermatheca    8.6   NA  Ovary    6.7   NA  Testis    2.6   NA  Male Accessory Gland    0.7   8.475  Carcass    2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    16 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    6 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    15 embryo 04-06hr    13 embryo 06-08hr    20 embryo 08-10hr    18 embryo 10-12hr    13 embryo 12-14hr    7 embryo 14-16hr    1 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • CG8704 RE34993 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010109 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8704-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for dpn Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | germline clone dpn6 lethal | polyphasic | recessive dpn1 lethal | recessive dpn1 dpn7 dpn8 partially lethal - majority live (with Df(2R)dpn-2) dpn7 Other Phenotypes Allele behavior defective | recessive dpn1 flightless (with Df(2R)dpn-2) dpn7 increased cell number dpnScer\UAS.cBa neuroanatomy defective | third instar larval stage, with Scer\GAL4elav.PU dpnScer\UAS.cSa neuroanatomy defective | third instar larval stage, with Scer\GAL4wor.PA dpnScer\UAS.cSa size defective | third instar larval stage, with Scer\GAL4elav.PU dpnScer\UAS.cSa uncoordinated (with Df(2R)dpn-2) dpn7 Phenotype manifest in Allele embryonic/larval neuroblast | third instar larval stage dpnScer\UAS.cBa macrochaeta dpnBG02347 type II neuroblast | larval stage (with Df(2R)dpn-2) dpn7 type II neuroblast | larval stage | somatic clone dpn7 type II neuroblast | third instar larval stage, with Scer\GAL4elav.PU dpnScer\UAS.cSa type II neuroblast | third instar larval stage, with Scer\GAL4wor.PA dpnScer\UAS.cSa
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of dpn Class Mutagen Stocks Known lesion dpnMI00051 1 -- dpn1 amorphic allele - genetic evidence P-element activity 0 -- dpn3 loss of function allele gamma ray 0 Yes dpn4 loss of function allele gamma ray 0 Yes dpn5 loss of function allele gamma ray 0 Yes dpn6 loss of function allele gamma ray 0 Yes dpn7 antimorphic allele - genetic evidence gamma ray 0 Yes dpn8 spontaneous 0 -- dpnBG02347 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of dpn Class Mutagen Stocks Known lesion dpnJF02094 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes dpnKK101812 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes dpnGD1421 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes dpnhs.P in vitro construct - regulatory fusion 0 Yes dpnhs.PH in vitro construct - regulatory fusion 0 Yes dpnNIG.8704R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes dpnScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes dpnScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes dpnScer\UAS.T:Ecol\dam,T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Ab(2R)dpn8 (Barbash and Cline, 1995) Df(2R)1D6 (San-Juán and Baonza, 2011) Df(2R)44CE (Barbash and Cline, 1995) Df(2R)193A (Younger-Shepherd et al., 1992, Bier et al., 1992, Estes et al., 1995, Roark et al., 1995, Barbash and Cline, 1995) Df(2R)dpn-2 (Barbash and Cline, 1995) Df(2R)Exel6056 (Ryder, 2004.4.26) Df(2R)Exel6057 (Ryder, 2004.4.26)
Duplicated in	In(2LR)Scorv1LTE35B-4R (Younger-Shepherd et al., 1992)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1421} NA P{KK101812} NA P{NIG.8704R} NA P{TRiP.JF02094} NA P{UAS-dpn.B} NA P{UAS-dpn.Dam} NA P{UAS-dpn.S} NA reporter construct P{dpn.DEE.MutAT-lacZ} No P{dpn.DEE.MutLBS-lacZ} No P{dpn.DEE-lacZ} No P{dpn-lacZ.dA} No P{dpn-lacZ.dB} No P{dpn-lacZ.dC} No P{dpn-lacZ.dD} No P{dpn-lacZ.dE} No P{dpn-lacZ.dF} No P{dpn-lacZ.dG} No P{dpn-lacZ.dH} No P{dpn-lacZ.dI} No P{dpn-lacZ.dJ} No P{dpn-lacZ.dK} No P{dpn-lacZ.E1} No P{dpn-lacZ.E1-E2} No P{dpn-lacZ.E1-E2-E3} No P{dpn-lacZ.E1-E3} No P{dpn-lacZ.E2} No P{dpn-lacZ.E2-E3} No P{dpn-lacZ.E3} No P{dpn-lacZ.S} No GAL4 construct P{dpn-GAL4.B} No heat-shock construct P{HS-dpn} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}dpn1 No P{lacW}l(2)SH0598SH0598 No miscellaneous insertions Mi{MIC}dpnMI00051 NA P{GT1}dpnBG02347 NA insertion of mobile activating element P{GSV1}EP-3EP-3 NA insertion of enhancer trap binary system P{GalW}S443 No
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from direct assay (Southall and Brand, 2009) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription inferred from direct assay (Hoshijima et al., 1995)
Biological Process
CV term Evidence References
adult locomotory behavior inferred from mutant phenotype (Younger-Shepherd et al., 1992) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) dosage compensation inferred from mutant phenotype (Younger-Shepherd et al., 1992) larval locomotory behavior inferred from mutant phenotype (Younger-Shepherd et al., 1992) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (Hoshijima et al., 1995) Notch signaling pathway inferred from genetic interaction with N (San-Juán and Baonza, 2011) inferred from genetic interaction with Su(H) (San-Juán and Baonza, 2011) positive regulation of neuroblast proliferation inferred from mutant phenotype (Berger et al., 2012) sex determination, establishment of X:A ratio inferred from genetic interaction with Sxl AND inferred from mutant phenotype (Younger-Shepherd et al., 1992)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Bier et al., 1992)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from sequence or structural similarity with mouse Hes1 (FlyBase, 1992-) protein dimerization activity inferred from electronic annotation with InterPro:IPR011598 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity non-traceable author statement (Swiss-Prot Project Members, 1998.12.15)
Biological Process
CV term Evidence References
negative regulation of transcription, DNA-dependent non-traceable author statement (Schutt and Nothiger, 2000) negative regulation of transcription from RNA polymerase II promoter inferred from sequence or structural similarity with mouse Hes1 (FlyBase, 1992-) sex determination non-traceable author statement (Canon and Banerjee, 2000) sex determination, establishment of X:A ratio non-traceable author statement (Schutt and Nothiger, 2000)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0003677 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
dpn - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG12343 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG17768 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG1832 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG2021 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG4849 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG5746 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG8801 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG8963 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CG9667 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CaBP1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - CkIIα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Clbn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Cypl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Ip259 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Map205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Nle anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Pdi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Rab5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - RpL7-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - RpLP0 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - RpS19b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - RpS27A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Su(var)205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - TFAM anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - U4-U6-60K anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Vha55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - dgrn pull down, autoradiography (Barry et al., 2011) dpn - flw anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - mRpL15 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - mRpL22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - polo anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - ppan anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - shrb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dpn - εCOP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions dpn allele Gene References dpn1 scrt (Roark et al., 1995) dpn3 da (Barbash and Cline, 1995) sc (Barbash and Cline, 1995) sisA (Barbash and Cline, 1995) dpn4 da (Barbash and Cline, 1995) sc (Barbash and Cline, 1995) sisA (Barbash and Cline, 1995) dpn5 da (Barbash and Cline, 1995) sc (Barbash and Cline, 1995) sisA (Barbash and Cline, 1995) dpn6 da (Barbash and Cline, 1995) sc (Barbash and Cline, 1995) sisA (Barbash and Cline, 1995) dpn7 da (Barbash and Cline, 1995) sc (Barbash and Cline, 1995) unspecified run (Sanchez et al., 1994) sisA (Sanchez et al., 1994, Barbash and Cline, 1995) Sxl (Sanchez et al., 1994)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61654 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010109 DroID - A comprehensive database of gene and protein interactions. • FBgn0010109 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35800
Orthologs
OrthoDB Orthologs (24) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6ZKK69 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6R22Q9 Arthropod inclusive ortholog search EOG69S4NS Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6ZKK69)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\dpn     Drosophila simulans   Dsim\GD15288 Y   Drosophila sechellia   Dsec\GM20707 Y   Drosophila erecta   Dere\GG10662 Y   Drosophila yakuba   Dyak\GE23198 Y   Drosophila ananassae   Dana\GF12602 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21268 Y   Drosophila persimilis   Dper\GL10878 Y   Drosophila willistoni   Dwil\GK15770 Y   Drosophila virilis   Dvir\GJ20653 Y   Drosophila mojavensis   Dmoj\GI20925 Y   Drosophila grimshawi   Dgri\GH20825 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6R22Q9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC724914   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010756   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH24741   Atta cephalotes Leafcutter ant  Acep\ACEP12985   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19755   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21468   Linepithema humile Argentine ant  Lhum\LH15732   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15678   Solenopsis invicta Red fire ant  Sinv\SINV16117   Pediculus humanus Human body louse  Phum\PHUM045420   Tribolium castaneum Red flour beetle  Tcas\TC005224
Orthologs in non-Insect Arthropods (EOG69S4NS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW016537
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15288 Drosophila sechellia Dsec\GM20707 Drosophila erecta Dere\GG10662 Drosophila yakuba Dyak\GE23198 Drosophila ananassae Dana\GF12602 Drosophila pseudoobscura pseudoobscura Dpse\GA21268 Drosophila persimilis Dper\GL10878 Drosophila willistoni Dwil\GK15770 Drosophila virilis Dvir\GJ20653 Drosophila mojavensis Dmoj\GI20925 Drosophila grimshawi Dgri\GH20825
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	26320 y1 v1; P{TRiP.JF02094}attP2 30603 y1 w*; Mi{MIC}dpnMI00051
VDRC	v106181 P{KK101812}VIE-260B
Genomic Clones ( 1 )
	BACR09N11 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE34993
Other clones	DPiM_RE34993
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE39164 RE49079
Other clones	67856 80721 214309.38 AA433195 EK022907 EK195730 EK213831 EP18240 RP003156025 RP003183504 RP003271686 RP003300831 RP003363614 RP003462399 RP003473185 RP003490183 RP003526994 RP003548799 RP003598234 RP003602703 RP003611003 RP003619595
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010109 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35800
Antibody Information
	monoclonal (Boone and Doe, 2008) polyclonal (Bier et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: dpn anon-EST:fe1B12 (Bayraktaroglu, 2000.11.2)
Additional comments
Other Comments
	dpn mutants have defects in larval locomotion. (Parrish et al., 2006) dsRNA has been made from templates generated with primers directed against this gene. RNAi of dpn results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development. (Parrish et al., 2006) Expression of activated h in early blastoderm embryos causes ectopic Sxl expression and male-specific lethality, implying that the h-related denominator element dpn represses Sxl during sex-determination by directly recognising the early Sxl promoter. (Jimenez et al., 1996) An extensive genetic screen for dominant suppressors of the female specific lethal sisA mutation identified 10 alleles of dpn but no other loci. By itself dpn cannot account for the masculinizing effect of increased autosomal ploidy, but if other denominator elements exist their contributions must be less than that of dpn. The time course of expression of dpn and Sxl in dpn mutant backgrounds suggests that dpn is required for sex determination only during the later stages of X:A signalling in males, to prevent inappropriate expression of SxlPe in the face of increasing sis gene product levels. (Barbash and Cline, 1995) Ecol\lacZ reporter gene construct demonstrates dpn pan-neural enhancer is composed of CNS and PNS specific subelements. (Emery and Bier, 1995) dpn gene functions as a dose-dependent negative regulator of Sxl-Pe. (Estes et al., 1995) Transfection assays and in vitro DNA binding experiments indicate that da/sc heterodimers directly activate the Sxl early promoter by binding to both high and low affinity sites. dpn protein represses this activation by specific binding to a unique site within the Sxl early promoter. (Hoshijima et al., 1995) The yeast two hybrid system has been used to demonstrate specific interactions within the sisA, sc, dpn and da group of gene products, and to delimit their interaction domains. The results support and extend the model of the molecular basis of the X/A ratio signal. (Liu and Belote, 1995) Mutants at dpn show genetic interaction with achaete-scute complex mutants. (Bier et al., 1992) The dpn+ product is involved in mobility of larvae and adults. dpn may act as a denominator element in sex determination, with Sxl mediating male lethality due to imbalance of sc+, dpn+ and maternally provided emc+. (Younger-Shepherd et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057575 RefSeq (Proteins) • NP_476923 DDBJ / EMBL / GenBank • AA433195 AAB24149 AAF59113 AAL48952 AC008370 AY071330 AY863660 AY863661 AY863662 AY863663 AY863664 AY863665 AY863666 AY863667 AY863668 AY863669 AY863670 AY863671 AY863672 AY863673 AY863674 AY863675 AY863676 AY863677 AY863678 AY863679 AY863680 AY863681 AY863682 BI238511 S48025 Entrez Gene - A searchable database of RefSeq genes. • 35800 UniProtKB/Swiss-Prot • Q26263
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Orange subgroup (IPR018352) Orange (IPR003650) Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • CG8704 RE34993
Linkouts
	BioGRID - A database of protein and genetic interactions • 61654 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010109 DroID - A comprehensive database of gene and protein interactions. • FBgn0010109 DRSC - Results from RNAi screens. • FBgn0010109 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010109 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8704-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010109 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35800 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/deadpan.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35800 modMine - Data generated by the modENCODE project. • FBgn0010109 REDfly - A database of transcriptional regulatory elements. • FBgn0010109
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	44C (Wainwright and Ish-Horowicz, 1992, Vaessin et al., 1990) anon-EST:fe1B12   bHLHe50 (Stevens et al., 2008) CG8704 (Schulz et al., 2004, Norga et al., 2003, Hadar et al., 2012, Stevens et al., 2008, Stevens et al., 2008, Perkins et al., 2009.3.31, Krejcí et al., 2009, Keleman et al., 2009.8.5) Dpn (Wang et al., 2009, Quintero et al., 2005, Urbach et al., 2006, Kozhina et al., 2007, Parrish et al., 2006, Weng and Cohen, 2012, Weng et al., 2012, Wang et al., 2006, Baumgardt et al., 2009, Barry et al., 2011, Lee et al., 2006, Wang et al., 2007, Winston and Gottesfeld, 2000, Kawamori et al., 2011, Wang et al., 2011, Wang et al., 2007, Southall and Brand, 2009, Hwang and Rulifson, 2011, Bayraktar et al., 2010, Benito-Sipos et al., 2011, Lee et al., 2006, Bowman et al., 2008, Yasugi et al., 2008, Egger et al., 2007, Sousa-Nunes et al., 2009, Boone and Doe, 2008, Andrews et al., 2009, Chabu and Doe, 2008, Cabernard and Doe, 2009, Song and Lu, 2012, Wang et al., 2011, Weng et al., 2010, Siegrist et al., 2010, Berger et al., 2010, Karlsson et al., 2010, Reddy et al., 2010, Brankatschk and Eaton, 2010, Chang et al., 2010, Wang et al., 2011, Morante et al., 2011, Tan et al., 2011, Neumüller et al., 2011, Viktorin et al., 2011, Hirono et al., 2012, Zhu et al., 2011, Haenfler et al., 2012, Moraru et al., 2012, Xiao et al., 2012, Ouyang et al., 2011, Andersen et al., 2012, Callan et al., 2012) dpn (Reeves and Posakony, 2005, Bartolome and Charlesworth, 2006, Wallace et al., 2000, Hadar et al., 2012, San-Juán and Baonza, 2011, Macdonald and Long, 2005, Lu et al., 2008, Liang et al., 2008, Krejcí et al., 2009, Grad et al., 2004, Kang et al., 2009, Suissa et al., 2010, Molnar et al., 2006, Duncan and Dearden, 2010, Cave et al., 2011, Colonques et al., 2011, Gladstein et al., 2010, Hasegawa et al., 2011, Egger et al., 2010, Yasugi et al., 2010, Berger et al., 2012, Michaut et al., 2011, Kuzin et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Zacharioudaki et al., 2012, Kunz et al., 2012) DPN (Sprecher et al., 2006) unnamed (Schmid and Tautz, 1997)
Name Synonym	anon-fast-evolving-1B12   Deadpan (Richter et al., 2011, Sun et al., 2012, Baumgardt et al., 2009, Wang et al., 2007, Winston and Gottesfeld, 2000, Wang et al., 2007, Lee et al., 2006, Bowman et al., 2008, Sousa-Nunes et al., 2009, Boone and Doe, 2008, Andrews et al., 2009, Chabu and Doe, 2008, Morey et al., 2008, Song and Lu, 2012, Siegrist et al., 2010, Reddy et al., 2010, Morante et al., 2011, Beckervordersandforth et al., 2008, Tan et al., 2011, Hasegawa et al., 2011, Viktorin et al., 2011, Berger et al., 2012, Moraru et al., 2012, Andersen et al., 2012, Callan et al., 2012) deadpan (Younger-Shepherd et al., 1992, Kozhina et al., 2007, San-Juán and Baonza, 2011, Bonaccorsi et al., 2007, Kankel et al., 2007, Southall and Brand, 2009, Benito-Sipos et al., 2011, Choksi et al., 2006, Krejcí et al., 2009, Cabernard and Doe, 2009, Kang et al., 2009, Suissa et al., 2010, Chang et al., 2010, Kuzin et al., 2011)
Secondary FlyBase IDs
	FBgn0025271
References ( 222 )
Generate a list of	All references relating to this gene ( 222 )
List References by type	Research paper  ( 141 ) Supplementary material  ( 5 ) Review  ( 40 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 20 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 35 )
	Housden et al., 2013, PLoS Genet. 9(1): e1003162 Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. [FBrf0220474] Andersen et al., 2012, Dev. Biol. 363(1): 258--265 Sgt1 acts via an LKB1/AMPK pathway to establish cortical polarity in larval neuroblasts. [FBrf0217488] Berger et al., 2012, Cell Rep. 2(2): 407--418 FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. [FBrf0219320] Callan et al., 2012, Brain Res. 1462: 151--161 Fragile X Protein is required for inhibition of insulin signaling and regulates glial-dependent neuroblast reactivation in the developing brain. [FBrf0218552] Hadar et al., 2012, Gene 494(1): 65--72 A screen identifying genes responsive to Dpp and Wg signaling in the Drosophila developing wing. [FBrf0217293] Haenfler et al., 2012, Dev. Biol. 365(1): 219--228 Cortical aPKC kinase activity distinguishes neural stem cells from progenitor cells by ensuring asymmetric segregation of Numb. [FBrf0218950] Hirono et al., 2012, Gene Expr. Patterns 12(1-2): 11--17 Identification of hunchback cis-regulatory DNA conferring temporal expression in neuroblasts and neurons. [FBrf0217382] Kunz et al., 2012, Development 139(14): 2510--2522 Origin of Drosophila mushroom body neuroblasts and generation of divergent embryonic lineages. [FBrf0218649] Moraru et al., 2012, Neural Dev. 7(1): 14 Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila. [FBrf0218691] Song and Lu, 2012, J. Biol. Chem. 287(21): 17716--17728 Interaction of Notch Signaling Modulator Numb with α-Adaptin Regulates Endocytosis of Notch Pathway Components and Cell Fate Determination of Neural Stem Cells. [FBrf0218342] Sun et al., 2012, PLoS Genet. 8(2): e1002515 Neurophysiological Defects and Neuronal Gene Deregulation in Drosophila mir-124 Mutants. [FBrf0217508] Weng and Cohen, 2012, Development 139(8): 1427--1434 Drosophila miR-124 regulates neuroblast proliferation through its target anachronism. [FBrf0217785] Weng et al., 2012, Dev. Neurobiol. 72(11): 1376--1390 Changes in Notch signaling coordinates maintenance and differentiation of the Drosophila larval optic lobe neuroepithelia. [FBrf0219799] Xiao et al., 2012, Development 139(15): 2670--2680 klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division. [FBrf0218804] Zacharioudaki et al., 2012, Development 139(7): 1258--1269 bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation. [FBrf0217605] Barry et al., 2011, Development 138(9): 1759--1769 The Drosophila STUbL protein Degringolade limits HES functions during embryogenesis. [FBrf0213458] Benito-Sipos et al., 2011, Development 138(24): 5311--5320 Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development. [FBrf0216799] Cave et al., 2011, Mol. Cell. Biol. 31(1): 22--29 Differential Regulation of Transcription through Distinct Suppressor of Hairless DNA Binding Site Architectures during Notch Signaling in Proneural Clusters. [FBrf0212524] Colonques et al., 2011, PLoS ONE 6(4): e19342 A Transient Expression of Prospero Promotes Cell Cycle Exit of Drosophila Postembryonic Neurons through the Regulation of Dacapo. [FBrf0213608] Hasegawa et al., 2011, Development 138(5): 983--993 Concentric zones, cell migration and neuronal circuits in the Drosophila visual center. [FBrf0213020] Hwang and Rulifson, 2011, Development 138(14): 2883--2893 Serial specification of diverse neuroblast identities from a neurogenic placode by Notch and Egfr signaling. [FBrf0214015] Kawamori et al., 2011, Dev. Growth Differ. 53(5): 653--667 Fat / Hippo pathway regulates the progress of neural differentiation signaling in the Drosophila optic lobe. [FBrf0213932] Kuzin et al., 2011, Mech. Dev. 128(3-4): 165--177 Functional analysis of conserved sequences within a temporally restricted neural precursor cell enhancer. [FBrf0213297] Michaut et al., 2011, Dev. Growth Differ. 53(9): 982--993 Analyzing the function of a hox gene: An evolutionary approach. [FBrf0216990] Morante et al., 2011, Development 138(4): 687--693 Cell migration in Drosophila optic lobe neurons is controlled by eyeless/Pax6. [FBrf0212874] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Ouyang et al., 2011, PLoS ONE 6(11): e28098 dp53 Restrains Ectopic Neural Stem Cell Formation in the Drosophila Brain in a Non-Apoptotic Mechanism Involving Archipelago and Cyclin E. [FBrf0216848] Richter et al., 2011, Nat. Cell Biol. 13(9): 1029--1039 The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements. [FBrf0215050] San-Juán and Baonza, 2011, Dev. Biol. 352(1): 70--82 The bHLH factor deadpan is a direct target of Notch signaling and regulates neuroblast self-renewal in Drosophila. [FBrf0213150] Tan et al., 2011, Development 138(11): 2197--2206 Coordinated expression of cell death genes regulates neuroblast apoptosis. [FBrf0213685] Viktorin et al., 2011, Dev. Biol. 356(2): 553--565 Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development. [FBrf0214495] Wang et al., 2011, Biochem. Biophys. Res. Commun. 410(4): 714--720 Role of JAK/STAT signaling in neuroepithelial stem cell maintenance and proliferation in the Drosophila optic lobe. [FBrf0214416] Wang et al., 2011, Dev. Cell 21(3): 520--533 An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. [FBrf0215580] Wang et al., 2011, Dev. Biol. 350(2): 414--428 Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development. [FBrf0212909] Zhu et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(51): 20615--20620 Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains. [FBrf0216951] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...