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General Information
Symbol
Dmel\dpn
Species
D. melanogaster
Name
deadpan
Annotation Symbol
CG8704
Feature Type
FlyBase ID
FBgn0010109
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
44C
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:8,228,971..8,232,330 [-]
Recombination map
2-58
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS -
Basic helix-loop-helix (bHLH) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60 amino acid region comprising a basic DNA binding domain followed by a HLH motif formed from two amphipathic α-helices connected by a loop. bHLH transcription factors form homo- and hetero-dimeric complexes, which bind to a E box consensus sequence. (Adapted from PMID:15186484).
Protein Function (UniProtKB)
Transcriptional repressor of genes that require a bHLH protein for their transcription (PubMed:1427077, PubMed:28899667, PubMed:24618901). In the larval brain, required to maintain the self-renewal and identity of type II neuroblasts by regulating the expression of the transcriptional repressor erm together with other self-renewal transcriptional repressors such as klu and E(spl)mgamma-HLH (PubMed:28899667, PubMed:23056424, PubMed:21262215, PubMed:24618901, PubMed:22357926). As part of its role in neuroblasts development, has been shown to be a direct target of the Notch signaling pathway, however might work also independently of N/Notch (PubMed:21262215, PubMed:22357926, PubMed:23056424). In the developing larval and pupal brain, required for mushroom body differentiation (PubMed:22357926). Involved in sex determination and SXL transcription repression when in complex with the corepressor protein Groucho (PubMed:8001118, PubMed:7651341).
(UniProt, Q26263)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\dpn or the JBrowse view of Dmel\dpn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.49
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088803
2036
435
Additional Transcript Data and Comments
Reported size (kB)
2.3 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087879
46.6
435
7.55
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Homodimer (PubMed:22357926, PubMed:7651341). Heterodimer with E(spl)mgamma-HLH and E(spl) (PubMed:22357926). Transcription repression requires formation of a complex with the corepressor protein Groucho (PubMed:8001118). Interacts (via bHLH motif) with sisA (PubMed:7651341). Interacts with da (PubMed:7651341).
(UniProt, Q26263)
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
(UniProt, Q26263)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dpn using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (22 terms)
Molecular Function (8 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR011598
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
Biological Process (13 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from genetic interaction with UniProtKB:P29617
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:P51023
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:M9PF84
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:Q94517
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:Q8MQJ9
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:Q9VQ56
(assigned by UniProt )
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000105428
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000105428
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000105428
(assigned by GO_Central )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
dpn is expressed in precellular blastoderm embryos during cycle 13 in a nearly ubiquitous pattern. It is then expressed in a pattern of 8 pair rule stripes. In late germband extension, expression in a rosette pattern is observed. At stage 10 expression is seen in the neuroblasts of each hemisegment along the ventral nerve cord.
No dpn transcripts are detected in dpn1 mutant embryos.
dpn transcripts are first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn transcripts are expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and become restricted to the neuroblasts before they delaminate. By 6 1/2-7 hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of nervous system development of the CNS and PNS as neurons begin to differentiate. dpn is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In ventral thoracic discs, expression is also observed in additional non-neuronal cells. dpn expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background.
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
neuroblast

Comment: Assayed at ~5hr after pupal formation.

neuroblast

Comment: Assayed at ~30hr after pupal formation.

neuroblast

Comment: Assayed at mid-pupal stage.

Additional Descriptive Data
dpn-protein expression can be found in neuroblasts in the distal part of the inner proliferation zone in the larval optic anlage.
dpn protein is localized to type II and secondary neuroblasts, but not to type I neuroblasts.
Approximately 100 neuroblasts in the 96hr 3rd instar larvae central nervous system express dpn. At ~5hr after pupal formation, the number of cells expressing dpn remains the same as in 3rd instar larvae, but cell size and mitotic activity are reduced. Midway through pupal development, only the mushroom body neuroblasts remain. Expression is no longer present 10hr before eclosion (~96hr APF).
dpn is expressed weakly in two to three rows of neural progenitor cells lateral to the l(1)sc-expressing cells in the developing optic lobe and at high levels in the neuroblasts.
No dpn protein is detected in neural precursors in dpn1 mutants.
dpn protein is first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn protein is expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and is restricted to the neuroblasts before they delaminate. By 6 1/2-7hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn protein is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of CNS and PNS development as neurons begin to differentiate. dpn protein is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In leg imaginal discs, expression is also observed in additional non-neuronal cells. dpn protein expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background.
Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\dpn in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of dpn
Transgenic constructs containing regulatory region of dpn
Deletions and Duplications ( 8 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (13)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
8 of 15
Yes
No
 
6 of 15
No
No
4 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
 
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
6 of 15
No
No
4 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (11)
6 of 13
Yes
No
6 of 13
Yes
Yes
4 of 13
No
No
3 of 13
No
No
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (11)
6 of 12
Yes
Yes
5 of 12
No
No
4 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (27)
9 of 15
Yes
Yes
8 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
9 of 15
Yes
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190ABU )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150697 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0D7Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0D5R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GBN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (12)
7 of 10
5 of 10
5 of 10
4 of 10
3 of 10
3 of 10
2 of 10
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
model of  brain cancer
is ameliorated by eag1
is NOT ameliorated by Sh14
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Homodimer (PubMed:22357926, PubMed:7651341). Heterodimer with E(spl)mgamma-HLH and E(spl) (PubMed:22357926). Transcription repression requires formation of a complex with the corepressor protein Groucho (PubMed:8001118). Interacts (via bHLH motif) with sisA (PubMed:7651341). Interacts with da (PubMed:7651341).
(UniProt, Q26263 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-58
Cytogenetic map
Sequence location
2R:8,228,971..8,232,330 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
44C2-44C2
Limits computationally determined from genome sequence between P{lacW}Rs1k09514&P{lacW}l(2)k03110k03110 and P{lacW}Vps25k08904&P{lacW}ptck02507
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
44C-44C
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (12)
Genomic Clones (21)
cDNA Clones (26)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of
Source for merge of: dpn anon- EST:fe1B12
Additional comments
Other Comments
Host gene for two maternally inherited stable intronic sequence RNA (sisRNA), referred to as "sisR-4" and the "dpn 5' sisRNA".
dpn is both necessary and sufficient for maintaining neuroblast self-renewal.
dpn mutants have defects in larval locomotion.
dsRNA has been made from templates generated with primers directed against this gene. RNAi of dpn results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
Expression of activated h in early blastoderm embryos causes ectopic Sxl expression and male-specific lethality, implying that the h-related denominator element dpn represses Sxl during sex-determination by directly recognising the early Sxl promoter.
An extensive genetic screen for dominant suppressors of the female specific lethal sisA mutation identified 10 alleles of dpn but no other loci. By itself dpn cannot account for the masculinizing effect of increased autosomal ploidy, but if other denominator elements exist their contributions must be less than that of dpn. The time course of expression of dpn and Sxl in dpn mutant backgrounds suggests that dpn is required for sex determination only during the later stages of X:A signalling in males, to prevent inappropriate expression of SxlPe in the face of increasing sis gene product levels.
Ecol\lacZ reporter gene construct demonstrates dpn pan-neural enhancer is composed of CNS and PNS specific subelements.
dpn gene functions as a dose-dependent negative regulator of Sxl-Pe.
Transfection assays and in vitro DNA binding experiments indicate that da/sc heterodimers directly activate the Sxl early promoter by binding to both high and low affinity sites. dpn protein represses this activation by specific binding to a unique site within the Sxl early promoter.
The yeast two hybrid system has been used to demonstrate specific interactions within the sisA, sc, dpn and da group of gene products, and to delimit their interaction domains. The results support and extend the model of the molecular basis of the X/A ratio signal.
Mutants at dpn show genetic interaction with achaete-scute complex mutants.
The dpn+ product is involved in mobility of larvae and adults. dpn may act as a denominator element in sex determination, with Sxl mediating male lethality due to imbalance of sc+, dpn+ and maternally provided emc+.
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 59 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Linkouts
ApoDroso - Functional genomic database for photoreceptor development, survival and function
BioGRID - A database of protein and genetic interactions.
DPiM - Drosophila Protein interaction map
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
Dpn
(Bivik Stadler et al., 2019, Ramon-Cañellas et al., 2019, Zhang et al., 2019, Abed et al., 2018, Hakes et al., 2018, Schwartz and Rhiner, 2018, Shaikh and Tejedor, 2018, An et al., 2017, Caygill and Brand, 2017, Janssens et al., 2017, Mavromatakis and Tomlinson, 2017, Altenhein et al., 2016, Mukherjee et al., 2016, Shaikh et al., 2016, Apitz and Salecker, 2015, Arya et al., 2015, Fernández-Hernández and Rhiner, 2015, Jia et al., 2015, To et al., 2015, Eroglu et al., 2014, Kuang et al., 2014, Lai and Doe, 2014, Carney et al., 2013, Chai et al., 2013, Chen et al., 2013, Goh et al., 2013, Kux et al., 2013, Lee et al., 2013, Li et al., 2013, Pérez-Gómez et al., 2013, Suzuki et al., 2013, Wang et al., 2013, Zhou and Luo, 2013, Andersen et al., 2012, Callan et al., 2012, Haenfler et al., 2012, Hirono et al., 2012, Kelsom and Lu, 2012, Moraru et al., 2012, Song and Lu, 2012, Ulvklo et al., 2012, Weng and Cohen, 2012, Weng et al., 2012, Xiao et al., 2012, Barry et al., 2011, Benito-Sipos et al., 2011, Hwang and Rulifson, 2011, Kawamori et al., 2011, Morante et al., 2011, Neumüller et al., 2011, Ouyang et al., 2011, Tan et al., 2011, Viktorin et al., 2011, Wang et al., 2011, Wang et al., 2011, Wang et al., 2011, Weng and Lee, 2011, Zhu et al., 2011, Bayraktar et al., 2010, Berger et al., 2010, Brankatschk and Eaton, 2010, Chang et al., 2010, Karlsson et al., 2010, Monastirioti et al., 2010, Reddy et al., 2010, Siegrist et al., 2010, Sousa-Nunes et al., 2010, Weng et al., 2010, Andrews et al., 2009, Baumgardt et al., 2009, Cabernard and Doe, 2009, Sousa-Nunes et al., 2009, Southall and Brand, 2009, Wang et al., 2009, Boone and Doe, 2008, Bowman et al., 2008, Chabu and Doe, 2008, Yasugi et al., 2008, Egger et al., 2007, Kozhina et al., 2007, Wang et al., 2007, Wang et al., 2007, Lee et al., 2006, Lee et al., 2006, Parrish et al., 2006, Urbach et al., 2006, Wang et al., 2006, Quintero et al., 2005, Winston and Gottesfeld, 2000)
anon-EST:fe1B12
dpn
(Arya et al., 2019, Otsuki and Brand, 2019, Shokri et al., 2019, Tegeder et al., 2019, Aughey et al., 2018, Bischof et al., 2018, Li et al., 2018, Loewen et al., 2018, Mora et al., 2018, Paul et al., 2018, Pek, 2018, Reichardt et al., 2018, Vaufrey et al., 2018, Chan et al., 2017, Tay and Pek, 2017, Transgenic RNAi Project members, 2017-, Wu et al., 2017, Mavromatakis and Tomlinson, 2016, Urbach et al., 2016, Zacharioudaki et al., 2016, Gene Disruption Project members, 2015-, Huang et al., 2015, Loedige et al., 2015, Schertel et al., 2015, To et al., 2015, Awasaki et al., 2014, Awasaki et al., 2014, Ciglar et al., 2014, Córdoba and Estella, 2014, Eroglu et al., 2014, Janssens et al., 2014, Komori et al., 2014, Southall et al., 2014, Aleksic et al., 2013, Babaoğlan et al., 2013, Chai et al., 2013, Berger et al., 2012, Carney et al., 2012, Hadar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kunz et al., 2012, San Juan et al., 2012, Zacharioudaki et al., 2012, Zhu et al., 2012, Cave et al., 2011, Colonques et al., 2011, Hasegawa et al., 2011, Kappes et al., 2011, Kuzin et al., 2011, Michaut et al., 2011, Nien et al., 2011, San-Juán and Baonza, 2011, Duncan and Dearden, 2010, Egger et al., 2010, Gladstein et al., 2010, Suissa et al., 2010, Yasugi et al., 2010, Kang et al., 2009, Krejcí et al., 2009, Liang et al., 2008, Lu et al., 2008, Bartolome and Charlesworth, 2006, Molnar et al., 2006, Macdonald and Long, 2005, Reeves and Posakony, 2005, Schlatter and Maier, 2005, Grad et al., 2004, Wallace et al., 2000)
Secondary FlyBase IDs
  • FBgn0025271
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (347)