44C
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Gene model reviewed during 5.49
2.3 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
435 (aa)
Homodimer (PubMed:22357926, PubMed:7651341). Heterodimer with E(spl)mgamma-HLH and E(spl) (PubMed:22357926). Transcription repression requires formation of a complex with the corepressor protein Groucho (PubMed:8001118). Interacts (via bHLH motif) with sisA (PubMed:7651341). Interacts with da (PubMed:7651341).
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dpn using the Feature Mapper tool.
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: reported as ventral nerve cord anlage
Comment: reported as head epidermis primordium
Comment: reported as head epidermis primordium
Comment: reported as head epidermis primordium
dpn is expressed in precellular blastoderm embryos during cycle 13 in a nearly ubiquitous pattern. It is then expressed in a pattern of 8 pair rule stripes. In late germband extension, expression in a rosette pattern is observed. At stage 10 expression is seen in the neuroblasts of each hemisegment along the ventral nerve cord.
dpn transcripts are first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn transcripts are expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and become restricted to the neuroblasts before they delaminate. By 6 1/2-7 hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of nervous system development of the CNS and PNS as neurons begin to differentiate. dpn is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In ventral thoracic discs, expression is also observed in additional non-neuronal cells. dpn expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background.
Comment: 96 hours after hatching
Comment: Assayed at ~5hr after pupal formation.
Comment: Assayed at ~30hr after pupal formation.
Comment: Assayed at mid-pupal stage.
Comment: 36 hours after hatching
Comment: 48 hours after hatching
Comment: 72 hours APF
dpn-protein expression can be found in neuroblasts in the distal part of the inner proliferation zone in the larval optic anlage.
dpn protein is localized to type II and secondary neuroblasts, but not to type I neuroblasts.
Approximately 100 neuroblasts in the 96hr 3rd instar larvae central nervous system express dpn. At ~5hr after pupal formation, the number of cells expressing dpn remains the same as in 3rd instar larvae, but cell size and mitotic activity are reduced. Midway through pupal development, only the mushroom body neuroblasts remain. Expression is no longer present 10hr before eclosion (~96hr APF).
dpn protein is first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn protein is expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and is restricted to the neuroblasts before they delaminate. By 6 1/2-7hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn protein is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of CNS and PNS development as neurons begin to differentiate. dpn protein is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In leg imaginal discs, expression is also observed in additional non-neuronal cells. dpn protein expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background.
GBrowse - Visual display of RNA-Seq signals
View Dmel\dpn in GBrowse 22-58
2-58
2-57.4
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: dpn anon- EST:fe1B12
Host gene for two maternally inherited stable intronic sequence RNA (sisRNA), referred to as "sisR-4" and the "dpn 5' sisRNA".
dpn is both necessary and sufficient for maintaining neuroblast self-renewal.
dpn mutants have defects in larval locomotion.
dsRNA has been made from templates generated with primers directed against this gene. RNAi of dpn results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
An extensive genetic screen for dominant suppressors of the female specific lethal sisA mutation identified 10 alleles of dpn but no other loci. By itself dpn cannot account for the masculinizing effect of increased autosomal ploidy, but if other denominator elements exist their contributions must be less than that of dpn. The time course of expression of dpn and Sxl in dpn mutant backgrounds suggests that dpn is required for sex determination only during the later stages of X:A signalling in males, to prevent inappropriate expression of SxlPe in the face of increasing sis gene product levels.
Mutants at dpn show genetic interaction with achaete-scute complex mutants.