FB2018_06, released Dec 21, 2018 All FlyBase archives from FB2014_04 until FB2018_05 are temporarily unavailable.

Gene: Dmel\dpn

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General Information
Symbol
Dmel\dpn
Species
D. melanogaster
Name
deadpan
Annotation Symbol
CG8704
Feature Type
protein_coding_gene
FlyBase ID
FBgn0010109
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Snapshot
In progress.Contributions welcome.
Other Summaries
Also Known As
44C
Key Links
Genomic Location
Cytogenetic map
44C2-44C2
Sequence location
2R:8,228,971..8,232,330 [-]
Recombination map
2-58
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI Genome Data Viewer
UCSC Genome Browser
Ensembl Genome Browser
PopFly Genome Browser
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Protein Domains/Motifs
InterPro
Orange domain; Myc-type, basic helix-loop-helix (bHLH) domain; Helix-loop-helix DNA-binding domain superfamily
Molecular Function (see GO section for details)
Experimental Evidence
DNA binding; DNA-binding transcription factor activity; DNA-binding transcription repressor activity, RNA polymerase II-specific; enhancer binding; sequence-specific DNA binding; sequence-specific double-stranded DNA binding
Predictions / Assertions
DNA-binding transcription repressor activity, RNA polymerase II-specific; protein dimerization activity; RNA polymerase II regulatory region sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific double-stranded DNA binding; transcription corepressor activity; transcription factor binding
Summaries
Gene Group Membership
BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS -
Basic helix-loop-helix (bHLH) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60 amino acid region comprising a basic DNA binding domain followed by a HLH motif formed from two amphipathic α-helices connected by a loop. bHLH transcription factors form homo- and hetero-dimeric complexes, which bind to a E box consensus sequence. (Adapted from PMID:15186484).
UniProt Contributed Function Data
Transcriptional repressor of genes that require a bHLH protein for their transcription (PubMed:1427077, PubMed:28899667, PubMed:24618901). In the larval brain, required to maintain the self-renewal and identity of type II neuroblasts by regulating the expression of the transcriptional repressor erm together with other self-renewal transcriptional repressors such as klu and E(spl)mgamma-HLH (PubMed:28899667, PubMed:23056424, PubMed:21262215, PubMed:24618901, PubMed:22357926). As part of its role in neuroblasts development, has been shown to be a direct target of the Notch signaling pathway, however might work also independently of N/Notch (PubMed:21262215, PubMed:22357926, PubMed:23056424). In the developing larval and pupal brain, required for mushroom body differentiation (PubMed:22357926). Involved in sex determination and SXL transcription repression when in complex with the corepressor protein Groucho (PubMed:8001118, PubMed:7651341).
(UniProt, Q26263)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\dpn or the JBrowse view of Dmel\dpn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.49
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
dpn-RA
FBtr0088803
NM_057575
2036
435
Additional Transcript Data and Comments
Reported size (kB)
2.3 (northern blot)
(Bier et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
dpn-PA
FBpp0087879
46.6
435
7.55
NP_476923
AAF59113
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
435 (aa)
(Bier et al., 1992)
Comments
External Data
Subunit Structure (UniProtKB)
Homodimer (PubMed:22357926, PubMed:7651341). Heterodimer with E(spl)mgamma-HLH and E(spl) (PubMed:22357926). Transcription repression requires formation of a complex with the corepressor protein Groucho (PubMed:8001118). Interacts (via bHLH motif) with sisA (PubMed:7651341). Interacts with da (PubMed:7651341).
(UniProt, Q26263)
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
(UniProt, Q26263)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dpn using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (27 terms)
Molecular Function (10 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
DNA binding
inferred from direct assay
(Southall and Brand, 2009)
DNA-binding transcription factor activity
inferred from mutant phenotype
(assigned by UniProt )
(Li et al., 2017)
DNA-binding transcription repressor activity, RNA polymerase II-specific
inferred from direct assay
(Hoshijima et al., 1995)
enhancer binding
inferred from direct assay
(assigned by UniProt )
(Janssens et al., 2017)
sequence-specific DNA binding
inferred from direct assay
(assigned by UniProt )
(Janssens et al., 2017)
sequence-specific double-stranded DNA binding
inferred from direct assay
(assigned by UniProt )
(Li et al., 2017)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
DNA-binding transcription repressor activity, RNA polymerase II-specific
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
protein dimerization activity
inferred from electronic annotation with InterPro:IPR011598
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
RNA polymerase II regulatory region sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
sequence-specific double-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
transcription corepressor activity
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
transcription factor binding
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (16 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
adult locomotory behavior
inferred from mutant phenotype
(Younger-Shepherd et al., 1992)
dosage compensation
inferred from mutant phenotype
(Younger-Shepherd et al., 1992)
larval locomotory behavior
inferred from mutant phenotype
(Younger-Shepherd et al., 1992)
negative regulation of gene expression
inferred from mutant phenotype
(assigned by UniProt )
(Li et al., 2017)
negative regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(assigned by UniProt )
(Li et al., 2017)
inferred from direct assay
(Hoshijima et al., 1995)
neuroblast development
inferred from mutant phenotype
(assigned by UniProt )
(Zhu et al., 2012)
Notch signaling pathway
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
(San-Juán and Baonza, 2011)
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
(San-Juán and Baonza, 2011)
positive regulation of mitotic nuclear division
inferred from mutant phenotype
(Wallace et al., 2000)
positive regulation of neuroblast proliferation
inferred from mutant phenotype
(assigned by UniProt )
(Zacharioudaki et al., 2012, Zhu et al., 2012, San-Juán and Baonza, 2011)
inferred from mutant phenotype
(Wu et al., 2017, Berger et al., 2012)
inferred from genetic interaction with UniProtKB:P29617
(assigned by UniProt )
(Zhu et al., 2012)
regulation of neuroblast proliferation
inferred from mutant phenotype
(assigned by UniProt )
(Janssens et al., 2017, Li et al., 2017)
inferred from genetic interaction with UniProtKB:P51023
(assigned by UniProt )
(Li et al., 2017)
inferred from genetic interaction with UniProtKB:M9PF84
(assigned by UniProt )
(Janssens et al., 2017)
inferred from genetic interaction with UniProtKB:Q94517
(assigned by UniProt )
(Janssens et al., 2017)
inferred from genetic interaction with UniProtKB:Q8MQJ9
(assigned by UniProt )
(Janssens et al., 2014)
inferred from genetic interaction with UniProtKB:Q9VQ56
(assigned by UniProt )
(Janssens et al., 2014)
sex determination, establishment of X:A ratio
inferred from mutant phenotype
(Younger-Shepherd et al., 1992)
inferred from genetic interaction with FLYBASE:Sxl; FB:FBgn0264270
(Younger-Shepherd et al., 1992)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
cell differentiation
inferred from biological aspect of ancestor with PANTHER:PTN000105428
(assigned by GO_Central )
(Gaudet et al., 2011)
negative regulation of transcription, DNA-templated
non-traceable author statement
(Schutt and Nothiger, 2000)
Notch signaling pathway
inferred from biological aspect of ancestor with PANTHER:PTN000105428
(assigned by GO_Central )
(Gaudet et al., 2011)
regulation of neurogenesis
inferred from biological aspect of ancestor with PANTHER:PTN000105428
(assigned by GO_Central )
(Gaudet et al., 2011)
sex determination
non-traceable author statement
(Canon and Banerjee, 2000)
sex determination, establishment of X:A ratio
non-traceable author statement
(Schutt and Nothiger, 2000)
somitogenesis
inferred from biological aspect of ancestor with PANTHER:PTN000105429
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
nucleus
inferred from direct assay
(Bier et al., 1992)
inferred from mutant phenotype
(assigned by UniProt )
(Li et al., 2017)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000105427
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral neurectoderm
(Bier et al., 1992)
neuroblast
(Bier et al., 1992)
embryonic/larval neuron
(Bier et al., 1992)
embryonic stage & larval stage
peripheral nervous system
(Bier et al., 1992)
neuroblast
(Bier et al., 1992)
central nervous system
(Bier et al., 1992)
embryonic cycle 13
organism | gap expression pattern
(Bier et al., 1992)
organism | pair rule expression pattern
(Bier et al., 1992)
larval stage
external sensory organ
(Bier et al., 1992)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 6
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
mesectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
organism | pair rule expression pattern
(Fisher et al., 2012)
trunk mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 5 -- 6
mesectoderm | subset
(Kearney et al., 2004)
embryonic stage 7 -- 8
dorsal ectoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
mesectoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
embryonic stage 9
stage 1 neuroblast
(Emery and Bier, 1995)
embryonic stage 9 -- 10
stage 1 neuroblast
(Chang et al., 2000)
central brain primordium
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
embryonic stage 9 -- 12
midline primordium | subset
(Kearney et al., 2004)
embryonic stage 10 -- 12
sensory mother cell
(Emery and Bier, 1995)
embryonic stage 10 -- 13
neuroblast of ventral nerve cord primordium
(Emery and Bier, 1995)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
germline cell
(Fisher et al., 2012)
lateral cord glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
embryonic stage 13 -- 15
sensory neuron
(Emery and Bier, 1995)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
lateral cord glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
Additional Descriptive Data
No dpn transcripts are detected in dpn1 mutant embryos.
(Bier et al., 1992)
dpn transcripts are first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn transcripts are expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and become restricted to the neuroblasts before they delaminate. By 6 1/2-7 hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of nervous system development of the CNS and PNS as neurons begin to differentiate. dpn is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In ventral thoracic discs, expression is also observed in additional non-neuronal cells. dpn expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background.
(Bier et al., 1992)
Marker for
 
stage 1 neuroblast
(Chang et al., 2000)
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
embryonic/larval neuron
(Bier et al., 1992)
embryonic stage & larval stage
peripheral nervous system
(Bier et al., 1992)
embryonic cycle 13
organism | pair rule expression pattern
(Bier et al., 1992)
larval stage
external sensory organ
(Bier et al., 1992)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral neurectoderm
(Bier et al., 1992)
neuroblast
(Bier et al., 1992)
embryonic stage & larval stage
neuroblast
(Bier et al., 1992)
central nervous system
(Bier et al., 1992)
embryonic cycle 13
organism | gap expression pattern
(Bier et al., 1992)
embryonic stage 8
procephalic neurogenic region & Pcv7 neuroblast & Pcv9 neuroblast
(Urbach et al., 2003)
embryonic antennal segment & ventral neurectoderm & neuroblast
(Urbach et al., 2003)
embryonic stage 11
neuroblast NB6-4 | punctate
(Bernardoni et al., 1999)
embryonic stage 11 -- 16
neuroblast NB5-6 of thorax | subset of embryonic mesothoracic segment
(Ulvklo et al., 2012)
neuroblast NB5-6 of thorax | subset of embryonic metathoracic segment
(Ulvklo et al., 2012)
embryonic stage 17
mushroom body neuroblast
(Kraft et al., 2016)
embryonic neuroblast
(Kraft et al., 2016)
larval stage
secondary neuroblast
(Eroglu et al., 2014)
type II neuroblast
(Eroglu et al., 2014)
second instar larval stage
mushroom body neuroblast

Comment: 36 hours after hatching

(Kraft et al., 2016)
third instar larval stage
neuroblast of embryonic/larval optic lobe
(Weng et al., 2012)
inner proliferation zone | restricted
(Apitz and Salecker, 2015)
optic lobe forming neuroblast
(Southall et al., 2013)
early third instar larval stage
mushroom body neuroblast

Comment: 48 hours after hatching

(Kraft et al., 2016)
late third instar larval stage
neuroblast
(Siegrist et al., 2010)
mushroom body neuroblast

Comment: 96 hours after hatching

(Kraft et al., 2016)
prepupal stage
neuroblast

Comment: Assayed at ~5hr after pupal formation.

(Siegrist et al., 2010)
pupal stage
neuroblast

Comment: Assayed at ~30hr after pupal formation.

(Siegrist et al., 2010)
pharate adult stage
neuroblast

Comment: Assayed at mid-pupal stage.

(Siegrist et al., 2010)
pharate adult stage P9
mushroom body neuroblast

Comment: 72 hours APF

(Kraft et al., 2016)
Additional Descriptive Data
dpn-protein expression can be found in neuroblasts in the distal part of the inner proliferation zone in the larval optic anlage.
(Apitz and Salecker, 2015)
dpn protein is localized to type II and secondary neuroblasts, but not to type I neuroblasts.
(Eroglu et al., 2014)
Approximately 100 neuroblasts in the 96hr 3rd instar larvae central nervous system express dpn. At ~5hr after pupal formation, the number of cells expressing dpn remains the same as in 3rd instar larvae, but cell size and mitotic activity are reduced. Midway through pupal development, only the mushroom body neuroblasts remain. Expression is no longer present 10hr before eclosion (~96hr APF).
(Siegrist et al., 2010)
dpn protein is first detected in a ubiquitous pattern in embryonic cycle 12. A transient gap-rule pattern in cycle 13 is followed by a pair-rule stripe pattern. dpn stripes overlap with the corresponding h stripes which extend further posteriorly. dpn protein is expressed briefly in patches of neurectoderm preceding the first wave of neuroblast segregation and is restricted to the neuroblasts before they delaminate. By 6 1/2-7hr, all neuroblasts have delaminated and express dpn. In the PNS, dpn protein is expressed weakly in patches of ectodermal cells followed by strong expression in sensillum precursors. Expression in neural precursors disappears soon after they divide and reappears at later stages of CNS and PNS development as neurons begin to differentiate. dpn protein is also expressed in neuroblasts of the larval CNS and in precursors of sensory neurons in imaginal discs. Expression is restricted to the primary neural precursor cell. In leg imaginal discs, expression is also observed in additional non-neuronal cells. dpn protein expression is absent in neuroblasts in a da mutant background, but is present at normal levels in a reduced number of neuroblasts in a Df(1)sc-B57 background.
(Bier et al., 1992)
No dpn protein is detected in neural precursors in dpn1 mutants.
(Bier et al., 1992)
Marker for
 
neuroblast
(Kraft et al., 2016)
secondary neuroblast
(Eroglu et al., 2014)
type II neuroblast
(Eroglu et al., 2014)
Subcellular Localization
CV Term
Evidence
References
nucleus
inferred from direct assay
(Bier et al., 1992)
inferred from mutant phenotype
(assigned by UniProt )
(Li et al., 2017)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\dpn in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Allele of dpn
Class
Mutagen
Associated Insertion
Stocks
Known lesion
dpn1
amorphic allele - genetic evidence
0
--
dpn7
antimorphic allele - genetic evidence
0
Yes
dpnMI00051
    1
    Yes
    dpn6
    loss of function allele
    0
    Yes
    dpnMI00051-GFSTF.0
    1
    Yes
    dpn3
    loss of function allele
    0
    Yes
    dpn4
    loss of function allele
    0
    Yes
    dpn5
    loss of function allele
    0
    Yes
    dpn8
    0
    --
    dpnBG02347
    0
    Yes
    dpnP82
      0
      Yes
      Other relevant insertions
      insertion of mobile activating element
      Name
      Expression Data
      P{GSV1}EP-3EP-3
      NA
      miscellaneous insertions
      Name
      Expression Data
      P{GalW}S443
      NA
      P{lacW}l(2)SH0598SH0598
      NA
      Transgenic Constructs ( 19 )
      For All Alleles Carried on Transgenic Constructs Show
      Transgenic constructs containing/affecting coding region of dpn
      Allele of dpn
      Mutagen
      Associated Transgenic Construct
      Stocks
      dpnKK101812
      1
      dpnUAS.cWa
      0
      dpnJF02094
      1
      dpnUAS.ORF.GW.Tag:HA
      1
      dpnNIG.8704R
      0
      dpnUAS.cBa
      0
      dpnUAS.cSa
      0
      dpnUAS.cVa
      0
      dpnHMC03154
      1
      dpnsfGFP,Tag:FLAG,Tag:CS(PSP),Tag:CS(TEVp),Tag:BLRP
      1
      dpnTOE.GS02055
      1
      dpnTOE.GS02409
      1
      dpnVSH330362
      1
      dpnGD1421
      0
      dpnhs.P
      0
      dpnintron2.UAS.Tag:MYC,DsRed(Unk)
      0
      dpnshRNA.UAS
      0
      dpnUAS.cUa
      0
      dpnUAS.Tag:Dam,Tag:MYC
      0
      Transgenic constructs containing regulatory region of dpn
      characterization construct
      Name
      Expression Data
      P{dpn(-FRT)cre.PEST}
      NA
      P{dpn(-FRT)LexA.p65}
      NA
      P{dpn(-KDRT)cre.PEST}
      NA
      GAL4 construct
      Name
      Expression Data
      P{dpn-GAL4.B}
      No
      vital-reporter construct
      Name
      Expression Data
      P{dpn-GFP.a}
      No
      P{dpn-GFP.b}
      No
      reporter construct
      Name
      Expression Data
      P{dpn.DEE.MutAT-lacZ}
      No
      P{dpn.DEE.MutLBS-lacZ}
      No
      P{dpn.DEE-lacZ}
      No
      P{dpn-lacZ.dA}
      Yes
      P{dpn-lacZ.dB}
      No
      P{dpn-lacZ.dC}
      Yes
      P{dpn-lacZ.dD}
      Yes
      P{dpn-lacZ.dE}
      No
      P{dpn-lacZ.dF}
      Yes
      P{dpn-lacZ.dG}
      No
      P{dpn-lacZ.dH}
      No
      P{dpn-lacZ.dI}
      Yes
      P{dpn-lacZ.dJ}
      No
      P{dpn-lacZ.dK}
      No
      P{dpn-lacZ.E1}
      No
      P{dpn-lacZ.E1-E2}
      No
      P{dpn-lacZ.E1-E2-E3}
      No
      P{dpn-lacZ.E1-E3}
      No
      P{dpn-lacZ.E2}
      No
      P{dpn-lacZ.E2-E3}
      No
      P{dpn-lacZ.E3}
      No
      P{dpn-lacZ.L}
      No
      P{dpn-lacZ.S}
      No
      Deletions and Duplications ( 8 )
      Summary of Phenotypes
      For more details about a specific phenotype click on the relevant allele symbol.
      Lethality
      Allele
      cell lethal | germline clone
      lethal | recessive
      lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4insc-Mz1407
      partially lethal - majority live (with Df(2R)dpn-2)
      viable
      Other Phenotypes
      Allele
      behavior defective | recessive
      cell death defective, with Scer\GAL4insc-Mz1407
      decreased cell number | larval stage
      flightless (with Df(2R)dpn-2)
      increased cell number
      increased cell number | larval stage | somatic clone, with Scer\GAL4insc-Mz1407
      neuroanatomy defective, with Scer\GAL4GMR9D11
      neuroanatomy defective | adult stage, with Scer\GAL471B
      neuroanatomy defective | adult stage, with Scer\GAL4insc-Mz1407, Scer\GAL80ase.PZ
      neuroanatomy defective | larval stage
      neuroanatomy defective | larval stage, with Scer\GAL4insc-Mz1407
      neuroanatomy defective | larval stage (with dpn1)
      neuroanatomy defective | larval stage (with dpn7)
      neuroanatomy defective | larval stage | somatic clone, with Scer\GAL4insc-Mz1407
      neuroanatomy defective | somatic clone
      neuroanatomy defective | third instar larval stage
      neuroanatomy defective | third instar larval stage, with Scer\GAL471B
      neuroanatomy defective | third instar larval stage, with Scer\GAL4elav.PU
      neuroanatomy defective | third instar larval stage, with Scer\GAL4insc-Mz1407
      neuroanatomy defective | third instar larval stage, with Scer\GAL4insc-Mz1407, Scer\GAL80ase.PZ
      neuroanatomy defective | third instar larval stage, with Scer\GAL4wor.PA
      size defective | third instar larval stage, with Scer\GAL4elav.PU
      some die during embryonic stage
      some die during larval stage
      some die during pupal stage
      uncoordinated (with Df(2R)dpn-2)
      visible, with Scer\GAL4Bx-MS1096
      visible, with Scer\GAL4en-e16E
      Phenotype manifest in
      Allele
      adult brain, with Scer\GAL471B
      embryonic/larval neuroblast | third instar larval stage
      embryonic/larval optic lobe | third instar larval stage
      embryonic/larval optic lobe | third instar larval stage, with Scer\GAL471B
      ganglion mother cell | larval stage | supernumerary, with Scer\GAL4ase.neuro
      ganglion mother cell | larval stage | supernumerary, with Scer\GAL4insc-Mz1407
      lamina | third instar larval stage
      macrochaeta
      mushroom body neuroblast | larval stage (with dpn1)
      mushroom body neuroblast | larval stage (with dpn7)
      mushroom body neuroblast | somatic clone
      secondary neuroblast | larval stage | supernumerary, with Scer\GAL4insc-Mz1407
      secondary neuroblast | supernumerary, with Scer\GAL4insc-Mz1407, Scer\GAL80ase.PZ
      type II neuroblast, with Scer\GAL4GMR9D11
      type II neuroblast | larval stage
      type II neuroblast | larval stage (with Df(2R)dpn-2)
      type II neuroblast | larval stage (with dpn1)
      type II neuroblast | larval stage (with dpn7)
      type II neuroblast | larval stage | somatic clone
      type II neuroblast | larval stage | somatic clone | supernumerary, with Scer\GAL4insc-Mz1407
      type II neuroblast | supernumerary, with Scer\GAL4insc-Mz1407, Scer\GAL80ase.PZ
      type II neuroblast | third instar larval stage, with Scer\GAL4elav.PU
      type II neuroblast | third instar larval stage, with Scer\GAL4insc-Mz1407
      type II neuroblast | third instar larval stage, with Scer\GAL4wor.PA
      type I neuroblast | larval stage | supernumerary, with Scer\GAL4ase.neuro
      type I neuroblast | larval stage | supernumerary, with Scer\GAL4insc-Mz1407
      type I neuroblast | third instar larval stage, with Scer\GAL4insc-Mz1407
      type I neuroblast | third instar larval stage (with dpn1)
      type I neuroblast | third instar larval stage (with dpn7)
      wing, with Scer\GAL4Bx-MS1096
      wing, with Scer\GAL4en-e16E
      Orthologs
      Downloads
      Download All DIOPT Orthologs
      Download All OrthoDB Orthologs
      Human Orthologs (via DIOPT v7.1)
      Homo sapiens (Human) (13)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Source
      Compara
      eggNOG
      Hieranoid
      Homologene
      Inparanoid
      Isobase
      OMA
      OrthoDB
      OrthoFinder
      OrthoInspector
      orthoMCL
      Panther
      Phylome
      RoundUp
      TreeFam
      ZFIN
      Alignment
      Complementation?
      Transgene?
      Hsap\HES1
      8 of 15
      Yes
      Yes
      Hsap\HES4
      8 of 15
      Yes
      No
       
      Hsap\HES2
      6 of 15
      No
      No
      Hsap\HES6
      4 of 15
      No
      No
      Hsap\BHLHE40
      2 of 15
      No
      Yes
      Hsap\BHLHE41
      2 of 15
      No
      Yes
       
      Hsap\HEYL
      2 of 15
      No
      No
      Hsap\HELT
      1 of 15
      No
      No
      Hsap\HES3
      1 of 15
      No
      Yes
      Hsap\HES5
      1 of 15
      No
      No
      Hsap\HES7
      1 of 15
      No
      Yes
      Hsap\HEY1
      1 of 15
      No
      No
      Hsap\HEY2
      1 of 15
      No
      No
      Model Organism Orthologs (via DIOPT v7.1)
      Mus musculus (laboratory mouse) (12)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Source
      Compara
      eggNOG
      Hieranoid
      Homologene
      Inparanoid
      Isobase
      OMA
      OrthoDB
      OrthoFinder
      OrthoInspector
      orthoMCL
      Panther
      Phylome
      RoundUp
      TreeFam
      ZFIN
      Alignment
      Complementation?
      Transgene?
      Mmus\Hes1
      8 of 15
      Yes
      Yes
      Mmus\Hes2
      6 of 15
      No
      No
      Mmus\Hes6
      4 of 15
      No
      No
      Mmus\Bhlhe40
      2 of 15
      No
      Yes
      Mmus\Bhlhe41
      2 of 15
      No
      Yes
      Mmus\Hes7
      2 of 15
      No
      Yes
      Mmus\Heyl
      2 of 15
      No
      No
      Mmus\Helt
      1 of 15
      No
      No
      Mmus\Hes3
      1 of 15
      No
      Yes
      Mmus\Hes5
      1 of 15
      No
      No
      Mmus\Hey1
      1 of 15
      No
      No
      Mmus\Hey2
      1 of 15
      No
      No
      Rattus norvegicus (Norway rat) (11)
      Rnor\Hes1
      6 of 13
      Yes
      No
      Rnor\Hes2
      6 of 13
      Yes
      Yes
      Rnor\Hes6
      4 of 13
      No
      No
      Rnor\Heyl
      3 of 13
      No
      No
      Rnor\Bhlhe40
      2 of 13
      No
      Yes
      Rnor\Bhlhe41
      2 of 13
      No
      Yes
      Rnor\Hes7
      2 of 13
      No
      Yes
      Rnor\Hey2
      2 of 13
      No
      No
      Rnor\Helt
      1 of 13
      No
      No
      Rnor\Hes3
      1 of 13
      No
      Yes
      Rnor\Hes5
      1 of 13
      No
      Yes
      Xenopus tropicalis (Western clawed frog) (11)
      Xtro\hes1
      6 of 12
      Yes
      Yes
      Xtro\hes4
      5 of 12
      No
      No
      Xtro\hes2
      4 of 12
      No
      Yes
      Xtro\hes6.1
      2 of 12
      No
      No
      Xtro\hes6.2
      2 of 12
      No
      No
      Xtro\bhlhe40
      1 of 12
      No
      Yes
      Xtro\bhlhe41
      1 of 12
      No
      Yes
      Xtro\hes7.1
      1 of 12
      No
      No
      Xtro\hes7.2
      1 of 12
      No
      Yes
      Xtro\hey1
      1 of 12
      No
      No
      Xtro\hey2
      1 of 12
      No
      No
      Danio rerio (Zebrafish) (27)
      Drer\her9
      9 of 15
      Yes
      Yes
      Drer\her6
      8 of 15
      No
      Yes
      Drer\hes2.1
      5 of 15
      No
      Yes
      Drer\her8.2
      4 of 15
      No
      No
      Drer\her8a
      4 of 15
      No
      No
      Drer\hes2.2
      4 of 15
      No
      Yes
      Drer\her13
      3 of 15
      No
      No
      Drer\hes6
      3 of 15
      No
      No
      Drer\bhlhe40
      2 of 15
      No
      Yes
      Drer\bhlhe41
      2 of 15
      No
      Yes
      Drer\her1
      2 of 15
      No
      Yes
      Drer\her5
      2 of 15
      No
      Yes
      Drer\her11
      2 of 15
      No
      No
      Drer\heyl
      2 of 15
      No
      No
      Drer\helt
      1 of 15
      No
      No
      Drer\her2
      1 of 15
      No
      Yes
      Drer\her3
      1 of 15
      No
      Yes
      Drer\her4.2
      1 of 15
      No
      Yes
      Drer\her4.3
      1 of 15
      No
      Yes
      Drer\her4.4
      1 of 15
      No
      Yes
      Drer\her7
      1 of 15
      No
      No
      Drer\her12
      1 of 15
      No
      No
      Drer\her15.1
      1 of 15
      No
      No
      Drer\her15.2
      1 of 15
      No
      No
      Drer\hey1
      1 of 15
      No
      No
      Drer\hey2
      1 of 15
      No
      No
      Drer\LOC100149863
      1 of 15
      No
      Yes
      Caenorhabditis elegans (Nematode, roundworm) (4)
      Cele\lin-22
      9 of 15
      Yes
      Yes
      Cele\F52G2.3
      1 of 15
      No
      Yes
      Cele\hlh-25
      1 of 15
      No
      Yes
      Cele\hlh-27
      1 of 15
      No
      Yes
      Arabidopsis thaliana (thale-cress) (1)
      Atha\AT3G56770
      1 of 9
      Yes
      Yes
      Saccharomyces cerevisiae (Brewer's yeast) (1)
      Scer\PHO4
      1 of 15
      Yes
      Yes
      Schizosaccharomyces pombe (Fission yeast) (0)
      No orthologs reported.
      Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190ABU )
      Organism
      Common Name
      Gene
      AAA Syntenic Ortholog
      Multiple Dmel Genes in this Orthologous Group
      Drosophila melanogaster
      fruit fly
      Dmel\dpn
      Drosophila suzukii
      Spotted wing Drosophila
      Dsuz\DS10_00006730
      Drosophila simulans
      Dsim\GD15288
      Drosophila sechellia
      Dsec\GM20707
      Drosophila erecta
      Dere\GG10662
      Drosophila yakuba
      Dyak\GE23198
      Drosophila ananassae
      Dana\GF12602
      Drosophila pseudoobscura pseudoobscura
      Dpse\GA21268
      Drosophila persimilis
      Dper\GL10878
      Drosophila willistoni
      Dwil\GK15770
      Drosophila virilis
      Dvir\GJ20653
      Drosophila mojavensis
      Dmoj\GI20925
      Drosophila grimshawi
      Dgri\GH20825
      Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150697 )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Musca domestica
      House fly
      Mdoa\17016651
      Glossina morsitans
      Tsetse fly
      Gmor\GMOY010652
      Lucilia cuprina
      Australian sheep blowfly
      Lcup\FF38_08755
      Mayetiola destructor
      Hessian fly
      Mdes\Mdes005350
      Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0D7Z )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Bombyx mori
      Silkmoth
      Bmor\BGIBMGA005390
      Danaus plexippus
      Monarch butterfly
      Dple\DPOGS200811-PA
      Heliconius melpomene
      Postman butterfly
      Hmel\HMEL011919
      Apis florea
      Little honeybee
      Aflo\5873079476
      Apis florea
      Little honeybee
      Aflo\3768605124
      Apis mellifera
      Western honey bee
      Amel\LOC724914
      Apis mellifera
      Western honey bee
      Amel\GB47799
      Bombus impatiens
      Common eastern bumble bee
      Bimp\BIMP16036
      Bombus impatiens
      Common eastern bumble bee
      Bimp\BIMP16047
      Bombus terrestris
      Buff-tailed bumblebee
      Bter\15202709
      Bombus terrestris
      Buff-tailed bumblebee
      Bter\15202706
      Linepithema humile
      Argentine ant
      Lhum\LH18324
      Linepithema humile
      Argentine ant
      Lhum\LH15732
      Megachile rotundata
      Alfalfa leafcutting bee
      Mrot\MROTU:0012cb
      Megachile rotundata
      Alfalfa leafcutting bee
      Mrot\MROTU:0012d9
      Nasonia vitripennis
      Parasitic wasp
      Nvit\Nasvi2EG010756
      Nasonia vitripennis
      Parasitic wasp
      Nvit\Nasvi2EG010770
      Dendroctonus ponderosae
      Mountain pine beetle
      Dpod\YQE_12940
      Dendroctonus ponderosae
      Mountain pine beetle
      Dpod\YQE_08150
      Tribolium castaneum
      Red flour beetle
      Tcas\TC005224
      Tribolium castaneum
      Red flour beetle
      Tcas\TC012851
      Pediculus humanus
      Human body louse
      Phum\PHUM045420
      Pediculus humanus
      Human body louse
      Phum\PHUM170110
      Rhodnius prolixus
      Kissing bug
      Rpro\RPRC000496
      Cimex lectularius
      Bed bug
      Clec\CLEC009505
      Cimex lectularius
      Bed bug
      Clec\CLEC003006
      Acyrthosiphon pisum
      Pea aphid
      Apim\ACYPI004499
      Zootermopsis nevadensis
      Nevada dampwood termite
      Znev\Znev_07982
      Zootermopsis nevadensis
      Nevada dampwood termite
      Znev\Znev_07981
      Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0D5R )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Strigamia maritima
      European centipede
      Smar\SMAR001692
      Strigamia maritima
      European centipede
      Smar\SMAR012956
      Strigamia maritima
      European centipede
      Smar\SMAR012957
      Ixodes scapularis
      Black-legged tick
      Isca\ISCW016537
      Ixodes scapularis
      Black-legged tick
      Isca\ISCW016540
      Stegodyphus mimosarum
      African social velvet spider
      Smim\KFM73076.1
      Stegodyphus mimosarum
      African social velvet spider
      Smim\KFM81513.1
      Tetranychus urticae
      Two-spotted spider mite
      Turt\tetur08g02740
      Tetranychus urticae
      Two-spotted spider mite
      Turt\tetur08g02780
      Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GBN )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Strongylocentrotus purpuratus
      Purple sea urchin
      Spur\Sp-Hairy2/4
      Strongylocentrotus purpuratus
      Purple sea urchin
      Spur\Sp-HesC
      Ciona intestinalis
      Vase tunicate
      Cint\e(spl)/hairy-b
      Ciona intestinalis
      Vase tunicate
      Cint\e(spl)/hairy-c
      Gallus gallus
      Domestic chicken
      Ggal\ENSGALG00000028015
      Gallus gallus
      Domestic chicken
      Ggal\HEYL
      Gallus gallus
      Domestic chicken
      Ggal\ENSGALG00000002055
      Human Disease Model Data
      FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 1 )
      Allele
      Disease
      Evidence
      References
      dpnUAS.cWa
      model of  brain cancer
      inferred from mutant phenotype
      (Huang et al., 2015)
      Interactions ( 1 )
      Allele
      Disease
      Interaction
      References
      dpnUAS.cWa
      model of  brain cancer
      is ameliorated by eagDN.EKI.UAS
      (Huang et al., 2015)
      is ameliorated by eag1
      (Huang et al., 2015)
      is NOT ameliorated by Sh14
      (Huang et al., 2015)
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      HES1; hes family bHLH transcription factor 1
      8 of 15
      139605
        HES4; hes family bHLH transcription factor 4
        8 of 15
        608060
           
          HES2; hes family bHLH transcription factor 2
          6 of 15
          609970
            HES6; hes family bHLH transcription factor 6
            4 of 15
            610331
              Functional Complementation Data
              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
              Interactions
              Summary of Physical Interactions
              esyN Network Diagram
              Show neighbor-neighbor interactions:
              Select Layout:
              Legend:
              Protein
              RNA
              Selected Interactor(s)
              Interactions Browser
              View in Interactions Browser
              Please look at the Interaction Group reports for full details of the physical interactions
              protein-protein
              Interacting group
              Assay
              References
              dpn
              two hybrid
              (Liu and Belote, 1995)
              dpn - CHOp24
              anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
              (Rhee et al., 2014)
              dpn - Df31
              anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
              (Rhee et al., 2014)
              dpn - E(spl)m8-HLH
              electrophoretic mobility shift assay, electrophoretic mobility supershift assay, autoradiography
              (Zacharioudaki et al., 2012)
              dpn - E(spl)mγ-HLH
              electrophoretic mobility shift assay, electrophoretic mobility supershift assay, autoradiography
              (Zacharioudaki et al., 2012)
              dpn - Sirt1
              pull down, autoradiography
              (Rosenberg and Parkhurst, 2002)
              dpn - da
              two hybrid
              (Liu and Belote, 1995)
              dpn - dgrn
              pull down, autoradiography
              (Barry et al., 2011)
              dpn - gro
              pull down, autoradiography, inferred by curator, two hybrid
              (Jennings et al., 2006, Poortinga et al., 1998, Paroush et al., 1994)
              dpn - h
              pull down, autoradiography
              (Secombe and Parkhurst, 2004)
              dpn - sisA
              two hybrid
              (Liu and Belote, 1995)
              RNA-protein
              Interacting group
              Assay
              References
              dpn - brat
              anti tag coimmunoprecipitation, primer specific pcr, electrophoretic mobility shift assay, autoradiography
              (Reichardt et al., 2018, Loedige et al., 2015)
              Summary of Genetic Interactions
              esyN Network Diagram
              esyN Network Key:
              Suppression
              Enhancement
              View in Interactions Browser
              Please look at the allele data for full details of the genetic interactions
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              dpn
              suppressible
              ban
              (Wu et al., 2017)
              dpn
              suppressible
              eag
              (Huang et al., 2015)
              dpn
              suppressible
              erm
              (Janssens et al., 2014)
              dpn
              enhanceable
              erm
              (Janssens et al., 2014)
              dpn
              suppressible
              pros
              (Zhu et al., 2012)
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              brat
              suppressible
              dpn
              (Reichardt et al., 2018)
              brat
              enhanceable
              dpn
              (Janssens et al., 2014)
              da
              suppressible
              dpn
              (Barbash and Cline, 1995)
              erm
              enhanceable
              dpn
              (Janssens et al., 2014)
              erm
              suppressible
              dpn
              (Janssens et al., 2014)
              N
              enhanceable
              dpn
              (Babaoğlan et al., 2013)
              N
              suppressible
              dpn
              (San-Juán and Baonza, 2011)
              sc
              suppressible
              dpn
              (Barbash and Cline, 1995)
              scrt
              enhanceable
              dpn
              (Roark et al., 1995)
              sisA
              suppressible
              dpn
              (Barbash and Cline, 1995)
              sisRNA:4
              suppressible
              dpn
              (Tay and Pek, 2017)
              External Data
              Subunit Structure (UniProtKB)
              Homodimer (PubMed:22357926, PubMed:7651341). Heterodimer with E(spl)mgamma-HLH and E(spl) (PubMed:22357926). Transcription repression requires formation of a complex with the corepressor protein Groucho (PubMed:8001118). Interacts (via bHLH motif) with sisA (PubMed:7651341). Interacts with da (PubMed:7651341).
              (UniProt, Q26263 )
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              Pathways
              Gene Group - Pathway Membership (FlyBase)
              External Data
              Linkouts
              Genomic Location and Detailed Mapping Data
              Chromosome (arm)
              2R
              Recombination map
              2-58
              Cytogenetic map
              44C2-44C2
              Sequence location
              2R:8,228,971..8,232,330 [-]
              FlyBase Computed Cytological Location
              Cytogenetic map
              Evidence for location
              44C2-44C2
              Limits computationally determined from genome sequence between P{lacW}Rs1k09514&P{lacW}l(2)k03110k03110 and P{lacW}Vps25k08904&P{lacW}ptck02507
              Experimentally Determined Cytological Location
              Cytogenetic map
              Notes
              References
              44C-44C
              (determined by in situ hybridisation)
              (Bier et al., 1992)
              Experimentally Determined Recombination Data
              Location
              2-58
              (FlyBase, 2016)
              2-57.4
              (Comeron et al., 2012)
              Left of (cM)
              Right of (cM)
              Notes
              Stocks and Reagents
              Stocks (10)
              Genomic Clones (21)
              cDNA Clones (26)
               

              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

              cDNA clones, fully sequences
              BDGP DGC clones
              Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Other Information
              Relationship to Other Genes
              Source for database identify of
              Source for database merge of
              Source for merge of: dpn anon- EST:fe1B12
              (Bayraktaroglu, 2000.11.2)
              Additional comments
              Other Comments
              Host gene for two maternally inherited stable intronic sequence RNA (sisRNA), referred to as "sisR-4" and the "dpn 5' sisRNA".
              (Tay and Pek, 2017)
              dpn is both necessary and sufficient for maintaining neuroblast self-renewal.
              (Zhu et al., 2012)
              dpn mutants have defects in larval locomotion.
              (Parrish et al., 2006)
              dsRNA has been made from templates generated with primers directed against this gene. RNAi of dpn results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
              (Parrish et al., 2006)
              Expression of activated h in early blastoderm embryos causes ectopic Sxl expression and male-specific lethality, implying that the h-related denominator element dpn represses Sxl during sex-determination by directly recognising the early Sxl promoter.
              (Jimenez et al., 1996)
              An extensive genetic screen for dominant suppressors of the female specific lethal sisA mutation identified 10 alleles of dpn but no other loci. By itself dpn cannot account for the masculinizing effect of increased autosomal ploidy, but if other denominator elements exist their contributions must be less than that of dpn. The time course of expression of dpn and Sxl in dpn mutant backgrounds suggests that dpn is required for sex determination only during the later stages of X:A signalling in males, to prevent inappropriate expression of SxlPe in the face of increasing sis gene product levels.
              (Barbash and Cline, 1995)
              Ecol\lacZ reporter gene construct demonstrates dpn pan-neural enhancer is composed of CNS and PNS specific subelements.
              (Emery and Bier, 1995)
              dpn gene functions as a dose-dependent negative regulator of Sxl-Pe.
              (Estes et al., 1995)
              Transfection assays and in vitro DNA binding experiments indicate that da/sc heterodimers directly activate the Sxl early promoter by binding to both high and low affinity sites. dpn protein represses this activation by specific binding to a unique site within the Sxl early promoter.
              (Hoshijima et al., 1995)
              The yeast two hybrid system has been used to demonstrate specific interactions within the sisA, sc, dpn and da group of gene products, and to delimit their interaction domains. The results support and extend the model of the molecular basis of the X/A ratio signal.
              (Liu and Belote, 1995)
              Mutants at dpn show genetic interaction with achaete-scute complex mutants.
              (Bier et al., 1992)
              The dpn+ product is involved in mobility of larvae and adults. dpn may act as a denominator element in sex determination, with Sxl mediating male lethality due to imbalance of sc+, dpn+ and maternally provided emc+.
              (Younger-Shepherd et al., 1992)
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 57 )
              Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              InterPro - A database of protein families, domains and functional sites
              Linkouts
              ApoDroso - Functional genomic database for photoreceptor development, survival and function
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
              DPiM - Drosophila Protein interaction map
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              FlyMine - An integrated database for Drosophila genomics
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Synonyms and Secondary IDs (10)
              Reported As
              Symbol Synonym
              44C
              (Wainwright and Ish-Horowicz, 1992, Vaessin et al., 1990)
              CG8704
              (Meadows, 2017.1.11, Hadar et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Krejcí et al., 2009, Perkins et al., 2009.3.31, Stevens et al., 2008, Stevens et al., 2008, Schlatter and Maier, 2005, Schulz et al., 2004, Norga et al., 2003)
              DPN
              (Sprecher et al., 2006)
              Dpn
              (Abed et al., 2018, Schwartz and Rhiner, 2018, Shaikh and Tejedor, 2018, An et al., 2017, Caygill and Brand, 2017, Janssens et al., 2017, Mavromatakis and Tomlinson, 2017, Altenhein et al., 2016, Mukherjee et al., 2016, Shaikh et al., 2016, Apitz and Salecker, 2015, Fernández-Hernández and Rhiner, 2015, Jia et al., 2015, To et al., 2015, Eroglu et al., 2014, Kuang et al., 2014, Lai and Doe, 2014, Carney et al., 2013, Chai et al., 2013, Chen et al., 2013, Goh et al., 2013, Kux et al., 2013, Lee et al., 2013, Li et al., 2013, Pérez-Gómez et al., 2013, Suzuki et al., 2013, Wang et al., 2013, Zhou and Luo, 2013, Andersen et al., 2012, Callan et al., 2012, Haenfler et al., 2012, Hirono et al., 2012, Kelsom and Lu, 2012, Moraru et al., 2012, Song and Lu, 2012, Ulvklo et al., 2012, Weng and Cohen, 2012, Weng et al., 2012, Xiao et al., 2012, Barry et al., 2011, Benito-Sipos et al., 2011, Hwang and Rulifson, 2011, Kawamori et al., 2011, Morante et al., 2011, Neumüller et al., 2011, Ouyang et al., 2011, Tan et al., 2011, Viktorin et al., 2011, Wang et al., 2011, Wang et al., 2011, Wang et al., 2011, Weng and Lee, 2011, Zhu et al., 2011, Bayraktar et al., 2010, Berger et al., 2010, Brankatschk and Eaton, 2010, Chang et al., 2010, Karlsson et al., 2010, Monastirioti et al., 2010, Reddy et al., 2010, Siegrist et al., 2010, Weng et al., 2010, Andrews et al., 2009, Baumgardt et al., 2009, Cabernard and Doe, 2009, Sousa-Nunes et al., 2009, Southall and Brand, 2009, Wang et al., 2009, Boone and Doe, 2008, Bowman et al., 2008, Chabu and Doe, 2008, Yasugi et al., 2008, Egger et al., 2007, Kozhina et al., 2007, Wang et al., 2007, Wang et al., 2007, Lee et al., 2006, Lee et al., 2006, Parrish et al., 2006, Urbach et al., 2006, Wang et al., 2006, Quintero et al., 2005, Winston and Gottesfeld, 2000)
              anon-EST:fe1B12
              bHLHe50
              (Stevens et al., 2008)
              dpn
              (Li et al., 2018, Mora et al., 2018, Pek, 2018, Reichardt et al., 2018, Chan et al., 2017, Tay and Pek, 2017, Transgenic RNAi Project members, 2017-, Wu et al., 2017, Mavromatakis and Tomlinson, 2016, Urbach et al., 2016, Zacharioudaki et al., 2016, Gene Disruption Project members, 2015-, Huang et al., 2015, Loedige et al., 2015, Schertel et al., 2015, To et al., 2015, Awasaki et al., 2014, Awasaki et al., 2014, Ciglar et al., 2014, Córdoba and Estella, 2014, Eroglu et al., 2014, Janssens et al., 2014, Komori et al., 2014, Southall et al., 2014, Aleksic et al., 2013, Babaoğlan et al., 2013, Chai et al., 2013, Berger et al., 2012, Carney et al., 2012, Hadar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kunz et al., 2012, San Juan et al., 2012, Zacharioudaki et al., 2012, Zhu et al., 2012, Cave et al., 2011, Colonques et al., 2011, Hasegawa et al., 2011, Kappes et al., 2011, Kuzin et al., 2011, Michaut et al., 2011, Nien et al., 2011, San-Juán and Baonza, 2011, Duncan and Dearden, 2010, Egger et al., 2010, Gladstein et al., 2010, Suissa et al., 2010, Yasugi et al., 2010, Kang et al., 2009, Krejcí et al., 2009, Liang et al., 2008, Lu et al., 2008, Bartolome and Charlesworth, 2006, Molnar et al., 2006, Macdonald and Long, 2005, Reeves and Posakony, 2005, Schlatter and Maier, 2005, Grad et al., 2004, Wallace et al., 2000)
              Name Synonyms
              Deadpan
              (Skeath et al., 2017, Mukherjee et al., 2016, Zacharioudaki et al., 2016, To et al., 2015, Jeibmann et al., 2014, Kuang et al., 2014, Lacin et al., 2014, Chai et al., 2013, Goh et al., 2013, Kux et al., 2013, Wang et al., 2013, Andersen et al., 2012, Berger et al., 2012, Callan et al., 2012, Kelsom and Lu, 2012, Moraru et al., 2012, Siddall et al., 2012, Song and Lu, 2012, Sun et al., 2012, Hasegawa et al., 2011, Morante et al., 2011, Richter et al., 2011, Tan et al., 2011, Viktorin et al., 2011, Reddy et al., 2010, Siegrist et al., 2010, Andrews et al., 2009, Baumgardt et al., 2009, Sousa-Nunes et al., 2009, Beckervordersandforth et al., 2008, Boone and Doe, 2008, Bowman et al., 2008, Chabu and Doe, 2008, Morey et al., 2008, Wang et al., 2007, Wang et al., 2007, Lee et al., 2006, Winston and Gottesfeld, 2000)
              anon-fast-evolving-1B12
              deadpan
              (Pek, 2018, Mavromatakis and Tomlinson, 2016, Urbach et al., 2016, Awasaki et al., 2014, Southall et al., 2014, Eguchi et al., 2013, San Juan et al., 2012, Yoshioka et al., 2012, Benito-Sipos et al., 2011, Kuzin et al., 2011, San-Juán and Baonza, 2011, Chang et al., 2010, Suissa et al., 2010, Cabernard and Doe, 2009, Kang et al., 2009, Krejcí et al., 2009, Southall and Brand, 2009, Bonaccorsi et al., 2007, Kankel et al., 2007, Kozhina et al., 2007, Choksi et al., 2006, Younger-Shepherd et al., 1992)
              Secondary FlyBase IDs
              • FBgn0025271
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (327)