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Gene Dmel\RpA-70

General Information
Symbol	Dmel\RpA-70	Species	D. melanogaster
Name	Replication Protein A 70	Annotation symbol	CG9633
Feature type	protein_coding_gene	FlyBase ID	FBgn0010173
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	RPA70, dRP-A, RPA, RP-A
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	84F6-84F6	Sequence location	3R:4,087,842..4,090,173 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Replication Protein A 70 is referred to in FlyBase by the symbol Dmel\RpA-70 (CG9633, FBgn0010173). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: single-stranded DNA binding. There is experimental evidence that it is involved in the biological process: neuron projection morphogenesis; DNA-dependent DNA replication; mitotic G2 DNA damage checkpoint; mitotic spindle organization. 7 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Nucleic acid binding, OB-fold, tRNA/helicase-type; Nucleic acid-binding, OB-fold; Replication factor A, C-terminal; Replication factor-A protein 1, N-terminal; Replication factor-a protein 1 Rpa1. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627380_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of RpA-70. Gene sequence location is 3R:4087842..4090173.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84F6-84F6   Limits computationally determined from genome sequence between P{PZ}grn05930 and P{PZ}Mcm2rL074
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3- (Perdigao, 1996.3.22)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RpA-70 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RpA-70-RA FBtr0081867  NM_079550   2135   603
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RpA-70-PA   FBpp0081356   66.6   603   6.73     NP_524274   AAF54206
Additional Polypeptide Data & Comments
Reported size (kDa)	70 (kD) (Mitsis et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Nucleic acid binding, OB-fold, tRNA/helicase-type (IPR004365) Replication factor-a protein 1 Rpa1 (IPR004591) Replication factor-A protein 1, N-terminal (IPR007199) Nucleic acid-binding, OB-fold (IPR012340) Replication factor A, C-terminal (IPR013955)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201663   AA391810   AA536473   AA540232   AA696553   AA940897   AA990775   AE014297 AAF54206   AI296889   AI516446   AI520301   AI533764   AI541654   AW941561   AY069331 AAL39476   BG637470   BG638979   BI166464   BI167444   BI214855   BI231222   BI240929   BI241869   BI356149   BI363066   BI373778   BI635188   BI636052   BP540436   BP540437   BP550876   BP550877   BP552977   CA806214   CA807721   CK660940   CO271849   CO275677   CO284563   CO306771   CO306790   CO308323   CO314000   CO319909   CO322321   CO336055   EC068832   EC071139   EC080383   EC198661   EC203393   EC209547   EC211405   EC222565   EC223714   EC226548   EC229251   EC232728   EC236251   EC237307   EC239112   EC261397   ES692492   EV580001   EV585849   GH754467   GH759116   GH759520   GH760347   GH776704   GH784395   GH785474   GH789448   GH790387   GH792275   GH799027   GH803607   GH808648   GH808925   GH811520   GH814783   GH816128   GH820263   GH820404   GH825311   GH827427   GH828020   GH829340   GH829515   GH833266   GH836046   GH836661   GH839603   GH841503   GH844059   GH849558   GH855031   GH861357   GH861567   GH865090   GH866884   GH879523   GH879909   GH880551   GH888377   GH888787   GH892549   GH895137   GH895398   GH896573   GH909771   GH910424   GH918310   GH926051   GH926465   GH932487   GH934768   GH934939   GH937675   GH937945   GH941067   GH942588   Z70277 CAA94241
UniProtKB/Swiss-Prot	Q24492
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium anterior endoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) Malpighian tubule primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic Malpighian tubule   (Fisher et al., 2012) embryonic/larval fat body   (Fisher et al., 2012) embryonic/larval lymph gland   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) gastric caecum   (Fisher et al., 2012) gonad   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
enzyme assay or biochemical detection Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Mitsis et al., 1993) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
	Purified from embryos. (Mitsis et al., 1993)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
DNA replication factor A complex inferred from direct assay (Mitsis et al., 1993) microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Mitsis, 1995)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary    1060.3 Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (696.725) Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary  (1060.3) Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary  1060.3 Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary    1060.3 Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary    1060.3 Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary  (1060.3) Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary  1060.3 Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    696.725 Larval Midgut    207.7 Larval Hindgut    262.1 Larval Malpighian Tubules    417 Larval Fat Body    212.5 Larval Salivary Gland    276.6 Larval Trachea    439.7 Larval Carcass    246.1 Adult Head    219.8 Adult Eye    179.5 Adult Brain    243.6 Adult Thoracic-Abdominal Ganglion    325.6 Adult Crop    325.1 Adult Midgut    248.4 Adult Hindgut    356.1 Adult Malpighian Tubules    376.9 Adult Fat Body    243.5 Adult Salivary Gland    242.5 Adult Heart    280.8 Adult VirginFemale Spermatheca    173.9 Adult InseminatedFemale Spermatheca    186.3 Adult Ovary    1060.3 Adult Testis    265.8 Adult Male Accessory Gland    152.3 Adult Carcass    239.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    219.8   NA  Eye    179.5   NA  Brain    243.6   696.725  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    325.6   NA  Crop    325.1   207.7  Midgut    248.4   262.1  Hindgut    356.1   417  Malpighian Tubules    376.9   212.5  Fat Body    243.5   276.6  Salivary Gland    242.5   NA  Heart    280.8   439.7  Trachea    NA   NA  VirginFemale Spermatheca    173.9   NA  InseminatedFemale Spermatheca    186.3   NA  Ovary    1060.3   NA  Testis    265.8   NA  Male Accessory Gland    152.3   246.1  Carcass    239.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female    116 ovary, mated 4-day female    92 testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (28) central nervous system, larvae L3  26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female  (116) ovary, mated 4-day female  (92) testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female  (116) ovary, mated 4-day female    92 testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female    116 ovary, mated 4-day female    92 testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female    116 ovary, mated 4-day female    92 testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  28 central nervous system, larvae L3  26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female  (116) ovary, mated 4-day female  (92) testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female  116 ovary, mated 4-day female    92 testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    26 central nervous system, pupae P8    10 head, virgin 1-day female    10 head, virgin 4-day female    6 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    6 head, mated 20-day female    7 head, mated 1-day male    8 head, mated 4-day male    10 head, mated 20-day male    17 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    4 fat body, white prepupae    6 fat body, pupae P8    3 carcass, larvae L3 wandering    18 carcass, 1-day adult    7 carcass, 4-day adult    11 carcass, 20-day adult    11 ovary, virgin 4-day female    116 ovary, mated 4-day female    92 testis, mated 4-day male    16 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    165 embryo 02-04hr    39 embryo 04-06hr    125 embryo 06-08hr    110 embryo 08-10hr    77 embryo 10-12hr    37 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    42 white prepupae 12hr    54 white prepupae 24hr    44 pupae 2d postWPP    27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day    27 adult female 01day    53 adult female 05day    102 adult female 30day    101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (165) embryo 02-04hr  (39) embryo 04-06hr  (125) embryo 06-08hr  (110) embryo 08-10hr  (77) embryo 10-12hr  (37) embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6  (27) larva L3 puffstage 7-9  (33) white prepupae new  (42) white prepupae 12hr  (54) white prepupae 24hr  (44) pupae 2d postWPP  (27) pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day  (27) adult female 01day  (53) adult female 05day  (102) adult female 30day  (101) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (165) embryo 02-04hr    39 embryo 04-06hr  (125) embryo 06-08hr  (110) embryo 08-10hr    77 embryo 10-12hr    37 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    42 white prepupae 12hr    54 white prepupae 24hr    44 pupae 2d postWPP    27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day    27 adult female 01day    53 adult female 05day  102 adult female 30day  101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    165 embryo 02-04hr    39 embryo 04-06hr    125 embryo 06-08hr    110 embryo 08-10hr    77 embryo 10-12hr    37 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    42 white prepupae 12hr    54 white prepupae 24hr    44 pupae 2d postWPP    27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day    27 adult female 01day    53 adult female 05day    102 adult female 30day    101 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    165 embryo 02-04hr    39 embryo 04-06hr    125 embryo 06-08hr    110 embryo 08-10hr    77 embryo 10-12hr    37 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    42 white prepupae 12hr    54 white prepupae 24hr    44 pupae 2d postWPP    27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day    27 adult female 01day    53 adult female 05day    102 adult female 30day    101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (165) embryo 02-04hr  (39) embryo 04-06hr  (125) embryo 06-08hr  (110) embryo 08-10hr  (77) embryo 10-12hr  (37) embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6  27 larva L3 puffstage 7-9  (33) white prepupae new  (42) white prepupae 12hr  (54) white prepupae 24hr  (44) pupae 2d postWPP  27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day  27 adult female 01day  (53) adult female 05day  (102) adult female 30day  (101) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (165) embryo 02-04hr    39 embryo 04-06hr  125 embryo 06-08hr  110 embryo 08-10hr    77 embryo 10-12hr    37 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    42 white prepupae 12hr    54 white prepupae 24hr    44 pupae 2d postWPP    27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day    27 adult female 01day    53 adult female 05day  102 adult female 30day  101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    165 embryo 02-04hr    39 embryo 04-06hr    125 embryo 06-08hr    110 embryo 08-10hr    77 embryo 10-12hr    37 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    13 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    20 larva L2    20 larva L3 12hr old    15 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    42 white prepupae 12hr    54 white prepupae 24hr    44 pupae 2d postWPP    27 pupae 3d postWPP    15 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    24 adult male 30day    27 adult female 01day    53 adult female 05day    102 adult female 30day    101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    134 Schneider line 2 Sg4    87 embryonic 1182-4H    86 embryonic GM2    154 embryonic Kc167    209 embryonic S1    97 embryonic S3    106 leg disc CME L1    82 wing disc CME-W2    200 wing disc ML-DmD8    88 wing disc ML-DmD9    80 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    68 wing disc ML-DmD32    79 haltere disc ML-DmD17-c3    106 eye-antennal disc ML-DmD11    139 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    87 CNS ML-DmBG2-c2    105 tumorous blood cells mbn2    70 ovary fGS/OSS    135 ovary OSC    73 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (134) Schneider line 2 Sg4  (87) embryonic 1182-4H  (86) embryonic GM2  (154) embryonic Kc167  (209) embryonic S1  (97) embryonic S3  (106) leg disc CME L1  (82) wing disc CME-W2  (200) wing disc ML-DmD8  (88) wing disc ML-DmD9  (80) wing disc ML-DmD16-c3  (87) wing disc ML-DmD21  (68) wing disc ML-DmD32  (79) haltere disc ML-DmD17-c3  (106) eye-antennal disc ML-DmD11  (139) antennal disc ML-DmD20-c5  (120) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1  (87) CNS ML-DmBG2-c2  (105) tumorous blood cells mbn2  (70) ovary fGS/OSS  (135) ovary OSC  (73) ovary OSS  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (134) Schneider line 2 Sg4    87 embryonic 1182-4H    86 embryonic GM2  (154) embryonic Kc167  (209) embryonic S1    97 embryonic S3  106 leg disc CME L1    82 wing disc CME-W2  (200) wing disc ML-DmD8    88 wing disc ML-DmD9    80 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    68 wing disc ML-DmD32    79 haltere disc ML-DmD17-c3  106 eye-antennal disc ML-DmD11  (139) antennal disc ML-DmD20-c5  (120) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1    87 CNS ML-DmBG2-c2  105 tumorous blood cells mbn2    70 ovary fGS/OSS  (135) ovary OSC    73 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    134 Schneider line 2 Sg4    87 embryonic 1182-4H    86 embryonic GM2    154 embryonic Kc167    209 embryonic S1    97 embryonic S3    106 leg disc CME L1    82 wing disc CME-W2    200 wing disc ML-DmD8    88 wing disc ML-DmD9    80 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    68 wing disc ML-DmD32    79 haltere disc ML-DmD17-c3    106 eye-antennal disc ML-DmD11    139 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    87 CNS ML-DmBG2-c2    105 tumorous blood cells mbn2    70 ovary fGS/OSS    135 ovary OSC    73 ovary OSS    40 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    134 Schneider line 2 Sg4    87 embryonic 1182-4H    86 embryonic GM2    154 embryonic Kc167    209 embryonic S1    97 embryonic S3    106 leg disc CME L1    82 wing disc CME-W2    200 wing disc ML-DmD8    88 wing disc ML-DmD9    80 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    68 wing disc ML-DmD32    79 haltere disc ML-DmD17-c3    106 eye-antennal disc ML-DmD11    139 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    87 CNS ML-DmBG2-c2    105 tumorous blood cells mbn2    70 ovary fGS/OSS    135 ovary OSC    73 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (134) Schneider line 2 Sg4  (87) embryonic 1182-4H  (86) embryonic GM2  (154) embryonic Kc167  (209) embryonic S1  (97) embryonic S3  (106) leg disc CME L1  (82) wing disc CME-W2  (200) wing disc ML-DmD8  (88) wing disc ML-DmD9  (80) wing disc ML-DmD16-c3  (87) wing disc ML-DmD21  (68) wing disc ML-DmD32  (79) haltere disc ML-DmD17-c3  (106) eye-antennal disc ML-DmD11  (139) antennal disc ML-DmD20-c5  (120) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1  (87) CNS ML-DmBG2-c2  (105) tumorous blood cells mbn2  (70) ovary fGS/OSS  (135) ovary OSC  (73) ovary OSS  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  134 Schneider line 2 Sg4    87 embryonic 1182-4H    86 embryonic GM2  (154) embryonic Kc167  (209) embryonic S1    97 embryonic S3  106 leg disc CME L1    82 wing disc CME-W2  (200) wing disc ML-DmD8    88 wing disc ML-DmD9    80 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    68 wing disc ML-DmD32    79 haltere disc ML-DmD17-c3  106 eye-antennal disc ML-DmD11  139 antennal disc ML-DmD20-c5  120 mixed discs ML-DmD4-c1  110 CNS ML-DmBG1-c1    87 CNS ML-DmBG2-c2  105 tumorous blood cells mbn2    70 ovary fGS/OSS  135 ovary OSC    73 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    134 Schneider line 2 Sg4    87 embryonic 1182-4H    86 embryonic GM2    154 embryonic Kc167    209 embryonic S1    97 embryonic S3    106 leg disc CME L1    82 wing disc CME-W2    200 wing disc ML-DmD8    88 wing disc ML-DmD9    80 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    68 wing disc ML-DmD32    79 haltere disc ML-DmD17-c3    106 eye-antennal disc ML-DmD11    139 antennal disc ML-DmD20-c5    120 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    87 CNS ML-DmBG2-c2    105 tumorous blood cells mbn2    70 ovary fGS/OSS    135 ovary OSC    73 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    28 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult  (34) Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult  (28) Cadmium 100 mM 48 hrs, 4-day adult  (68) Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult  (50) Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (73) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (59) Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult  (57) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    28 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    28 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    28 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult  (34) Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult  28 Cadmium 100 mM 48 hrs, 4-day adult  (68) Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult  (50) Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (73) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (59) Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult  (57) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    28 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    10 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    28 Cadmium 100 mM 48 hrs, 4-day adult    68 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    15 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    57 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD04815 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010173 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9633-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for RpA-70 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 RpA-70GD4228 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 RpA-70GD4228 viable RpA-70c01306 Sterility Allele fertile RpA-70c01306 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 RpA-70GD4228 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 RpA-70GD4228
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of RpA-70 Class Mutagen Stocks Known lesion RpA-70c01306 piggyBac transposase 2 -- RpA-70G5479 P-element activity 1 -- RpA-70MB09710 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RpA-70 Class Mutagen Stocks Known lesion RpA-70GD4228 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes RpA-70GL00350 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RpA-70KK101022 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RpA-70NIG.9633R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6147 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4228} NA P{KK101022} NA P{NIG.9633R} NA P{TRiP.GL00350} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}RpA-70c01306 NA insertion of enhancer trap binary system Mi{ET1}RpA-70MB09710 No insertion of mobile activating element P{EP}RpA-70G5479 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
single-stranded DNA binding inferred from direct assay (Mitsis et al., 1993)
Biological Process
CV term Evidence References
DNA-dependent DNA replication inferred from direct assay (Mitsis et al., 1993) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neuron projection morphogenesis inferred from mutant phenotype (Sepp et al., 2008)
Cellular Component
CV term Evidence References
DNA replication factor A complex inferred from direct assay (Mitsis et al., 1993) microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Mitsis, 1995)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
single-stranded DNA binding non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Biological Process
CV term Evidence References
DNA-dependent DNA replication non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Cellular Component
CV term Evidence References
DNA replication factor A complex non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) nucleus non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
RpA-70 - CG15220 experimental knowledge based (Guruharsha et al., 2011) RpA-70 - CG3165 experimental knowledge based (Guruharsha et al., 2011) RpA-70 - Gnf1 experimental knowledge based (Guruharsha et al., 2011) RpA-70 - Irbp experimental knowledge based (Guruharsha et al., 2011) RpA-70 - Ku80 experimental knowledge based (Guruharsha et al., 2011) RpA-70 - Lam experimental knowledge based (Guruharsha et al., 2011) RpA-70 - RPA2 experimental knowledge based (Guruharsha et al., 2011) RpA-70 - TFAM experimental knowledge based (Guruharsha et al., 2011) RpA-70 - psq experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RpA-70 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66153 DroID - A comprehensive database of gene and protein interactions. • FBgn0010173 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40972
Orthologs
OrthoDB Orthologs (51) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6BP1XC Dipteran inclusive ortholog search EOG67D9QB Insect inclusive ortholog search EOG6TQK5G Arthropod inclusive ortholog search EOG6TQJRD Metazoa inclusive ortholog search EOG6D2KH5
Orthologs in Drosophila Species (EOG6BP1XC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\RpA-70     Drosophila simulans   Dsim\GD20882 Y   Drosophila sechellia   Dsec\GM26360 Y   Drosophila erecta   Dere\GG17466 Y   Drosophila yakuba   Dyak\GE24866 Y   Drosophila ananassae   Dana\GF23244 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21928 Y   Drosophila persimilis   Dper\GL24182 Y   Drosophila willistoni   Dwil\GK11360 Y   Drosophila virilis   Dvir\GJ23056 Y   Drosophila mojavensis   Dmoj\GI24276     Drosophila grimshawi   Dgri\GH19242 Y
Orthologs in non-Drosophila Dipterans (EOG67D9QB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012826   Anopheles gambiae Malaria mosquito  Agam\AGAP001421   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002381
Orthologs in non-Dipteran Insects (EOG6TQK5G)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\RpA-70   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG028373   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004886   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16635   Atta cephalotes Leafcutter ant  Acep\ACEP15900   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16421   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14373   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21780   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16709   Linepithema humile Argentine ant  Lhum\LH23034   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13379   Solenopsis invicta Red fire ant  Sinv\SINV17205   Solenopsis invicta Red fire ant  Sinv\SINV14532   Solenopsis invicta Red fire ant  Sinv\SINV18167   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006150   Bombyx mori Silkmoth  Bmor\BGIBMGA005448   Pediculus humanus Human body louse  Phum\PHUM459200   Tribolium castaneum Red flour beetle  Tcas\TC011844   Tribolium castaneum Red flour beetle  Tcas\TC004405
Orthologs in non-Insect Arthropods (EOG6TQJRD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_306791   Daphnia pulex Water flea  Dpux\JGI_V11_249126   Ixodes scapularis Deer tick  Isca\ISCW020441   Ixodes scapularis Deer tick  Isca\ISCW007595
Orthologs in non-Arthropod Metazoa (EOG6D2KH5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\R03H10.6   Caenorhabditis elegans Nematode  Cele\R03H10.7   Caenorhabditis elegans Nematode  Cele\rpa-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001822gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028925gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005495gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012886gn   Danio rerio Zebrafish  Drer\rpa1   Xenopus tropicalis Western clawed frog  Xtro\rpa1   Gallus gallus Domestic chicken  Ggal\RPA1   Mus musculus House mouse  Mmus\Rpa1   Rattus norvegicus Norway rat  Rnor\Rpa1   Homo sapiens Human  Hsap\RPA1
Human Orthologs (1)
Gene OMIM HGNC Hsap\RPA1
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20882 Drosophila sechellia Dsec\GM26360 Drosophila erecta Dere\GG17466 Drosophila yakuba Dyak\GE24866 Drosophila ananassae Dana\GF23244 Drosophila pseudoobscura pseudoobscura Dpse\GA21928 Drosophila persimilis Dper\GL24182 Drosophila willistoni Dwil\GK11360 Drosophila virilis Dvir\GJ23056 Drosophila grimshawi Dgri\GH19242
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	10477 w1118; PBac{PB}RpA-70c01306 29057 w1118; Mi{ET1}RpA-70MB09710 35426 y1 sc* v1; P{TRiP.GL00350}attP2 30160 w1118; P{EP}RpA-70G5479/TM6C, Sb1
Harvard	- PBac{PB}RpA-70c01306
VDRC	v11210 w1118; P{GD4228}v11210 v11211 w1118; P{GD4228}v11211
Genomic Clones ( 1 )
	BACR45A07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 100 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD04815
Other clones	DPiM_LD04815
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM07873 LD10710 LD17095 LD19648 LD23223 LD34643 LD42320 LP10989 LP19920 RE06591 RE07832 RE20455 RE20992 RE37888 RE39060 RE42222 RE47783 RE61187 SD05722 SD05916 SD15479 SD16458 SD17617
Other clones	Dmel_dig_009652_0708_0411 Dmel_dig_016613_0705_3406 EK104933 EK118245 EK166315 EK187902 EK234642 EK252240 EK252403 EK257628 EK281355 EN11731 ESG012b.TE.2_F11 ESG019b.E21.2_B04 FGA004D06 FGM209F11 FGM221H05 LD40420 RP003062092 RP003070203 RP003073633 RP003111265 RP003142752 RP003153094 RP003156285 RP003168785 RP003173509 RP003189168 RP003195280 RP003199831 RP003222453 RP003225296 RP003267983 RP003284291 RP003299516 RP003316991 RP003321256 RP003322599 RP003325393 RP003347684 RP003351421 RP003353560 RP003380655 RP003382608 RP003389059 RP003397002 RP003401080 RP003402695 RP003404425 RP003426217 RP003458045 RP003463066 RP003475608 RP003477644 RP003490904 RP003511835 RP003513799 RP003523064 RP003525587 RP003538605 RP003547872 RP003553524 RP003563209 RP003572889 RP003586357 RP003604803 RP003613067 RP003627340 RP003637805 RP003639619 RP003662509 RP003671546 RP003673350 RP003679062 RP003679415
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010173 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40972
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RpA-70 CG9633 (Zhao, 1999.11)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) The Replication Protein A complex from ovaries and early embryos has multiple phosphorylated forms. The relative abundance of these forms is tightly regulated during development. The localization of the Replication Protein A complex varies during the cell cycle in both ovaries and embryos. (Mitsis, 1995) RpA-30, RpA-70 and RpA-8 can substitute for human RP-A for the initiation of SV40 DNA replication. (Kamakaka et al., 1994) Replication protein A has been purified and characterised. The protein consists of three subunits (encoded by RpA-30, RpA-70 and RpA-8), and binds single-stranded DNA very tightly via the large subunit (encoded by RpA-70). (Marton et al., 1994) Drosophila single stranded binding protein is a trimer with subunits of 70kD, 30kD and 8kD representing the cognates of the human and yeast SSB/RP-A proteins. (Mitsis et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079550 RefSeq (Proteins) • NP_524274 DDBJ / EMBL / GenBank • AA201663 AAF54206 AAL39476 AW941561 AY069331 CAA94241 Z70277 Entrez Gene - A searchable database of RefSeq genes. • 40972 UniProtKB/Swiss-Prot • Q24492
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Nucleic acid binding, OB-fold, tRNA/helicase-type (IPR004365) Replication factor-a protein 1 Rpa1 (IPR004591) Replication factor-A protein 1, N-terminal (IPR007199) Nucleic acid-binding, OB-fold (IPR012340) Replication factor A, C-terminal (IPR013955) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD04815
Linkouts
	BioGRID - A database of protein and genetic interactions • 66153 DroID - A comprehensive database of gene and protein interactions. • FBgn0010173 DRSC - Results from RNAi screens. • FBgn0010173 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010173 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9633-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010173 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40972 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40972 modMine - Data generated by the modENCODE project. • FBgn0010173
Synonyms & Secondary IDs ( 21 )
Reported As
Symbol Synonym	CG9633 (Battersby et al., 2003, Asha et al., 2003, Goshima et al., 2007, Hughes et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2011.7.19, Puri et al., 2008, Sepp et al., 2008) DmRPA (Takeuchi et al., 2004) dmrpa1 (Perdigao, 1996.3.22, ) dRP-A (Thommes et al., 1995, Marton et al., 1994) DRP-A (Mitsis, 1995) D-RPA70 (Takata et al., 2004) D-SSB (Mitsis et al., 1993) i164 (Battersby et al., 2003) RPA (Murawsky et al., 2003, Plank et al., 2006) RP-A (Oshige et al., 1999, Kamakaka et al., 1994) RPA1 (Somma et al., 2008) RPA 70 (Shimanouchi et al., 2003) Rpa-70 (Asha et al., 2003, Gruenewald et al., 2009) RPA70 (Leclerc et al., 1996, Kondo and Perrimon, 2011) RpA-70 (Lunardi et al., 2010, Goshima et al., 2007, Cayirlioglu et al., 2003, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Sepp et al., 2008) RpA70 (Somma et al., 2008, Puri et al., 2008) RPA 70 kDa (Crevel et al., 2011) Ssb-70
Name Synonym	Drosophila Replication Protein A (Mitsis, 1995) Replication Protein A 70 (Kondo and Perrimon, 2011) Single stranded-binding protein 70
Secondary FlyBase IDs
References ( 48 )
Generate a list of	All references relating to this gene ( 48 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 )
Recent research papers ( 2 )
	Crevel et al., 2011, PLoS ONE 6(11): e27101 Decreased MCM2-6 in Drosophila S2 Cells Does Not Generate Significant DNA Damage or Cause a Marked Increase in Sensitivity to Replication Interference. [FBrf0216797] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Show all research papers ...