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Gene Dmel\RpA-30

General Information
Symbol	Dmel\RpA-30	Species	D. melanogaster
Name	Replication Protein A 30	Annotation symbol
Feature type	gene	FlyBase ID	FBgn0010174
Gene Model Status	Unannotated	Stock availability	None publicly available
Also Known As	RPA30, dRP-A, RPA, RP-A
Genomic Location
Chromosome (arm)		Recombination map
Cytogenetic map		Sequence location
Summary Information
Automatically generated summary See sections below for more information	The gene Replication Protein A 30 is referred to in FlyBase by the symbol Dmel\RpA-30 (FBgn0010174). It is a gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: single-stranded DNA binding. There is experimental evidence that it is involved in the biological process: DNA-dependent DNA replication. No alleles are reported. It has no annotated transcripts. Gene has not been localized to the genome sequence.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein
Additional Polypeptide Data & Comments
Reported size (kDa)	30 (kD) (Mitsis et al., 1993)
Comments	RpA-30 protein, the 30kD subunit of single-stranded DNA-binding protein or SSB (a heterotrimer) was purified from 0-18hr embryos. SSB was characterized biochemically and was shown to stimulate the extent and processivity of DNA synthesis of its cognate DNA polymerase α. It is the Drosophila cognate of yeast and human SSB/RP-A proteins. (Mitsis et al., 1993)
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Mapped Features
Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
enzyme assay or biochemical detection Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Mitsis et al., 1993)
Additional Descriptive Data
	Purified from embryos. (Mitsis et al., 1993)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of RpA-30 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 0 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RpA-30 Class Mutagen Stocks Known lesion
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
single-stranded DNA binding inferred from direct assay (Mitsis et al., 1993)
Biological Process
CV term Evidence References
DNA-dependent DNA replication inferred from direct assay (Mitsis et al., 1993)
Cellular Component
CV term Evidence References
DNA replication factor A complex inferred from direct assay (Mitsis et al., 1993) nucleus inferred from direct assay (Mitsis, 1995)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RpA-30 allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 0 )
cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	The Replication Protein A complex from ovaries and early embryos has multiple phosphorylated forms. The relative abundance of these forms is tightly regulated during development. The localization of the Replication Protein A complex varies during the cell cycle in both ovaries and embryos. (Mitsis, 1995) RpA-30, RpA-70 and RpA-8 can substitute for human RP-A for the initiation of SV40 DNA replication. (Kamakaka et al., 1994) Replication protein A has been purified and characterised. The protein consists of three subunits (encoded by RpA-30, RpA-70 and RpA-8), and binds single-stranded DNA very tightly via the large subunit (encoded by RpA-70). (Marton et al., 1994) Drosophila single stranded binding protein is a trimer with subunits of 70kD, 30kD and 8kD representing the cognates of the human and yeast SSB/RP-A proteins. (Mitsis et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	DmRPA (Takeuchi et al., 2004) DmRP-A (Dusenbery and Smith, 1996) dRP-A (Thommes et al., 1995, Thommes et al., 1995, Marton et al., 1994) DRP-A (Mitsis, 1995) D-SSB (Mitsis et al., 1993) RPA (Murawsky et al., 2003, Plank et al., 2006) RP-A (Oshige et al., 1999, Kamakaka et al., 1994) RPA30 (Takata et al., 2004, Takata et al., 2002, Shimanouchi et al., 2003, Leclerc et al., 1996) RpA-30 (Cayirlioglu et al., 2003) Ssb-30
Name Synonym	Drosophila Replication Protein A (Mitsis, 1995) Replication Protein A 30   Single stranded-binding protein 30
Secondary FlyBase IDs
References ( 16 )
Research paper	Plank et al., 2006, Proc. Natl. Acad. Sci. USA 103(30): 11118--11123 Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration. [FBrf0191828] Takata et al., 2004, Genetics 168(2): 855--865 Drosophila damaged DNA-binding protein 1 is an essential factor for development. [FBrf0180301] Takeuchi et al., 2004, Biochem. J. 382(2): 535--543 Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography. [FBrf0179970] Cayirlioglu et al., 2003, Molec. Cell. Biol. 23(6): 2123--2134 Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. [FBrf0159210] Takata et al., 2002, Nucleic Acids Res. 30(17): 3795--3808 Drosophila damage-specific DNA-binding protein 1 (D-DDB1) is controlled by the DRE/DREF system. [FBrf0152283] Oshige et al., 1999, Mutat. Res. 433(3): 183--192 A new DNA polymerase species from Drosophila melanogaster: a probable mus308 gene product. [FBrf0108977] Leclerc et al., 1996, Molec. Biol. Cell 7(4): 505--513 Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II. [FBrf0087556] Mitsis, 1995, Dev. Biol. 170(2): 445--456 Phosphorylation and localization of replication protein A during oogenesis and early embryogenesis of Drosophila melanogaster. [FBrf0083305] Thommes et al., 1995, Nucleic Acids Res. 23(21): 4443--4450 Purification and characterisation of a DNA helicase, dhel I, from Drosophila melanogaster embryos. [FBrf0084443] Thommes et al., 1995, J. Biol. Chem. 270(36): 21137--21143 Mitochondrial single-stranded DNA-binding protein from Drosophila embryos. Physical and biochemical characterization. [FBrf0083479] Kamakaka et al., 1994, Molec. Cell. Biol. 14(8): 5114--5122 Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro. [FBrf0073501] Marton et al., 1994, FEBS Lett. 342(2): 139--144 Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster. [FBrf0068586] Mitsis et al., 1993, Biochemistry 32(19): 5257--5266 A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA. [FBrf0057831]
Review	Dusenbery and Smith, 1996, Mutat. Res. 364(3): 133--145 Cellular responses to DNA damage in Drosophila melanogaster. [FBrf0090538]
Abstract	Murawsky et al., 2003, A. Dros. Res. Conf. 44 Suppl.: 1028B Replication Protein A function in scs enhancer blocking and its recruitment to the borders of the 87A7 heat shock-induced puff. [FBrf0157109] Shimanouchi et al., 2003, A. Dros. Res. Conf. 44 Suppl.: 1018A Expression of Replication Protein A during development of D. melanogaster. [FBrf0157099]