DWnt5, DWnt-3, Dwnt-5, DWnt3, Wnt3
Wnt superfamily ligand involved in axon guidance and salivary gland morphogenesis - Wnt5 and Derailed gradients pattern the Drosophila olfactory dendritic map.
Please see the JBrowse view of Dmel\Wnt5 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
4.4 (northern blot)
3.8 (northern blot)
140 (kD observed)
1004 (aa); 112 (kD)
1010 (aa); 113 (kD predicted)
Interacts with porcupine (por).
Glycosylated, glycosylation is stimulated by porcupine at the ER.
Palmitoleoylated by porcupine. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport Wnt5 to unique sites at the cell surface. Depalmitoleoylated by notum, leading to inhibit Wnt signaling pathway.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Wnt5 using the Feature Mapper tool.
At stage 12, Wnt5 expression is observed in the epidermis, most prominently in two clusters, and throughout the somatic mesoderm that will give rise to the body wall musculature. At early stage 16, Wnt5 is present in the muscle attachment sites, and at low levels in contractile fibers in most muscles, including the A1-7 lateral transverse muscles 1, 2, and 3. At the end of embryonic development at late stage 17, Wnt5 is almost undetectable in the somatic mesoderm.
Wnt5 transcripts are distributed ubiquitously throughout the embryo until germ band extension. Then, specific staining can be seen in the nascent limb primordia of the head and thoracic segments and in stripes along the ventral neuroectoderm. Transcription in the limb primordia requires Dll activity.
Wnt5 transcript is detected in all developmental stages. In embryos, it is first detected in the cellular blastoderm stage, at 20-80% egg length. By gastrulation, the presumptive cephalic and ventral furrows express Wnt5 transcript. Mesodermal Wnt2 expression is first detected at stage 6, and continues at least until stage 15. At stages 10-11, the labial, maxillary, antennal and labral segments, and at stage 11, the thoracic leg primordia express Wnt5 transcript. Wnt5 expression in the CNS is detected starting at stage 11, through at least stage 16.
Comment: 6-24h APF
Comment: 48 APF
Wnt5-protein is detected at 16h APF throughout the developing antennal lobes with high concentrations in the anterior dorsolateral and posterior ventrolateral regions. Expression is also detected in a developing neuron projection bundle terminating at the dorsolateral edge of the developing adult antennal lobe. Wnt5-protein is co-expressed with Scer\GAL4GH146 and drl-protein in dendrites of a subset of antennal lobe projection neurons.
At stage 12, Wnt5 expression is observed in the epidermis, most prominently in two clusters, and throughout the somatic mesoderm that will give rise to the body wall musculature. At early stage 16, Wnt5 is present in the muscle attachment sites, and at low levels in contractile fibers in most muscles, including the A1-7 lateral transverse muscles 1, 2, and 3. At the end of embryonic development at late stage 17, Wnt5 is almost undetectable in the somatic mesoderm.
Wnt5 protein was localized to projection neuron dendrites during glomerular development.
Wnt5 protein is localized to neuronal cell bodies lateral to the midline of the ventral nerve cord in early embryonic stage 12. At stages embryonic stages 13-16, Wnt5 protein is found on longitudinal axons and on axons of the anterior and posterior commissures, with greatest abundance on the axons of the posterior commissures.
Wnt5 protein expression pattern resembles the Wnt5 transcript expression pattern. At stage 10, expression is detected in bilaterally symmetrical regions corresponding to the labrum and antennal primordia and the labial and maxillary gnathal segments. At stage 11, the protein is found in ventral patches of cells corresponding to the thoracic limb primordia, and in each neuromere. As the germ band retracts, expression is detected in the supraesophageal ganglia of the brain. By stage 13, expression is detected throughout the ventral nerve cord at low levels, with strong staining detected in each hemisegment in a pattern suggestive of the commissural and longitudinal axon bundles. Double staining with MAbBP102, which recognizes all neuronal cells, indicates that Wnt5 protein is expressed in neuronal cells.
Comment: 0h-6h APF
Comment: 16h-24h APF
GBrowse - Visual display of RNA-Seq signals
View Dmel\Wnt5 in GBrowse 21-60
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
polyclonal antibody
Source for identity of: Wnt5 CG6407
Source for merge of: Wnt5 anon-WO03040301.228
"l(1)G0265" may affect "Wnt5".
Source for merge of Wnt5 anon-WO03040301.228 was sequence comparison ( date:051113 ).
drl and Wnt5 are required (in the salivary gland and in the central nervous system respectively) for normal salivary gland migration in the embryo. They are required in the third phase of migration, to mediate the final positioning of the gland, as the salivary gland detaches from the circular visceral mesoderm and contacts the longitudinal visceral mesoderm.
Wnt5 is an anterograde signal that is expressed by olfactory receptor neurons. It is required to organise the glomerular pattern in the developing antennal lobe.
Wnt3 and Wnt5 are the same gene, and as the sequence is more similar to the vertebrate Wnt-5a and Wnt-5b genes the Wnt5 designation is more appropriate.
Sequence analysis of Wnt genes is performed in several species to determine the ancestral lineage of the gene family.