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Gene Dmel\Wnt5

General Information
Symbol	Dmel\Wnt5	Species	D. melanogaster
Name	Wnt oncogene analog 5	Annotation symbol	CG6407
Feature type	protein_coding_gene	FlyBase ID	FBgn0010194
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	DWnt-3, DWnt5, DWnt3, DWnt-3/5, DWnt-5, Wnt-3, Wnt3, DWnt3/5
Genomic Location
Chromosome (arm)	X	Recombination map	1-
Cytogenetic map	17B6-17C1	Sequence location	X:18,395,434..18,399,438 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Wnt oncogene analog 5 is referred to in FlyBase by the symbol Dmel\Wnt5 (CG6407, FBgn0010194). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: receptor binding. There is experimental evidence that it is involved in the biological process: negative chemotaxis; axon guidance; determination of muscle attachment site; Wnt receptor signaling pathway; axon extension; salivary gland morphogenesis. 23 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; multicellular structure; non-connected developing system; anatomical structure; eo support cell; embryonic/larval neuron; embryonic foregut; sense organ; adult segment. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Wnt; Wnt protein, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1635852_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Wnt5. Gene sequence location is X:18395589..18399436.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Polypeptides Wnt5-PB Transcripts Wnt5-RB
FB2013_02	Controlled Vocabulary Terms anterior/posterior pattern specification extracellular space frizzled-2 binding anterior/posterior axon guidance respiratory system development negative regulation of transcription from RNA polymerase II promoter dorsal/ventral axis specification canonical Wnt receptor signaling pathway plasma membrane
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 17B6-17C1   Limits computationally determined from genome sequence between P{EP}CG32549EP1317 and P{EP}EP443EP443&P{EP}BxEP1383
Experimentally Determined Cytological Location
Cytogenetic map Notes References 17A-17B     (Nusse, 1992.3.2) 17A-17B   (determined by in situ hybridisation)   (Fradkin et al., 1995) 17A-17B   (determined by in situ hybridisation)   (Russell et al., 1992) 17B1-17B2   (determined by in situ hybridisation)   (Eisenberg et al., 1992)
Experimentally Determined Recombination Data
Location	1- (Nusse, 1992.3.2)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Wnt5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.51 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Wnt5-RA FBtr0074623  NM_057576   3850   1004 Wnt5-RB FBtr0343046     4005   1004
Additional Transcript Data & Comments
Reported size (kB)	4.4 (northern blot) (Eisenberg et al., 1992) 3.8 (northern blot) (Russell et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Wnt5-PA   FBpp0074394   112.5   1004   9.02     NP_476924   AAF48853 Wnt5-PB   FBpp0309792   112.5   1004   9.02
Additional Polypeptide Data & Comments
Reported size (kDa)	1010 (aa); 113 (kD predicted) (Russell et al., 1992) 1004 (aa); 112 (kD) (Eisenberg et al., 1992) 140 (kD observed) (Fradkin et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Wnt protein, conserved site (IPR018161) Wnt (IPR005817)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA817354   AA820132   AA948927   AA978490   AA978629   AE014298 AAF48853   AC012098   AI456125   AX777374   AY061362   AZ877648   BG638323   BI161545   BI165940   BI231775   BI231990   BI241392   BI241469   BI362025   BI364541   BI365109   BI481572   BI482876   BI483038   BT010268 AAQ23586   CO177501   CO186451   CO273700   CO275843   CO278621   CO285430   CO295611   CO296817   CO309559   CO314139   CO316844   CO318424   CO336135   CO337645   CZ486618   DN154293   DQ138842 ABA86448   EC059921   EC060510   EC061197   EC066525   EC075906   EC087255   EC088248   EC201504   EC206259   EC235971   EC238026   EC245740   EC261574   EC262302   GH911830   M97450 AAA29020   X64736 CAA46002
UniProtKB/Swiss-Prot	P28466
UniProtKB/TrEMBL	Q2XY20
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage 9 -- 11   labial segment   (Eisenberg et al., 1992) maxillary lobe   (Eisenberg et al., 1992) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic brain   (Fradkin et al., 1995) ventral nerve cord   (Fradkin et al., 1995) embryonic stage 9 -- 11   antennal lobe   (Eisenberg et al., 1992) labral segment   (Eisenberg et al., 1992) embryonic stage 11   ventral metathoracic disc primordium   (Russell et al., 1992) ventral prothoracic disc primordium   (Russell et al., 1992) ventral mesothoracic disc primordium   (Russell et al., 1992) neuroblast of ventral nerve cord primordium   (Russell et al., 1992) ventral thoracic disc   (Eisenberg et al., 1992) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Russell et al., 1992) embryonic stage 5   organism | 20-80% egg length   (Russell et al., 1992) gastrula stage   ventral furrow | presumptive   (Russell et al., 1992) embryonic stage 6 -- 15   mesoderm   (Russell et al., 1992) embryonic stage 10 -- 11   antennal segment   (Russell et al., 1992) labial segment   (Russell et al., 1992) embryonic stage 14 -- 16   presumptive embryonic/larval central nervous system   (Russell et al., 1992)
Additional Descriptive Data
	Wnt5 transcript is detected in all developmental stages. In embryos, it is first detected in the cellular blastoderm stage, at 20-80% egg length. By gastrulation, the presumptive cephalic and ventral furrows express Wnt5 transcript. Mesodermal Wnt2 expression is first detected at stage 6, and continues at least until stage 15. At stages 10-11, the labial, maxillary, antennal and labral segments, and at stage 11, the thoracic leg primordia express Wnt5 transcript. Wnt5 expression in the CNS is detected starting at stage 11, through at least stage 16. (Russell et al., 1992) Wnt5 transcripts are distributed ubiquitously throughout the embryo until germ band extension. Then, specific staining can be seen in the nascent limb primordia of the head and thoracic segments and in stripes along the ventral neuroectoderm. Transcription in the limb primordia requires Dll activity. (Eisenberg et al., 1992) Wnt5 transcript expression pattern was compared to that of MAb44C11, a monoclonal antibody that recognizes nuclei throughout the brain and ventral nerve cord (as well as the PNS) . Wnt5 is found in all the CNS cells that stain with MAb44C11, but not in the PNS cells. (Fradkin et al., 1995)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10   antennal segment   (Fradkin et al., 1995) labial segment   (Fradkin et al., 1995) maxillary segment   (Fradkin et al., 1995) labral segment   (Fradkin et al., 1995) embryonic stage 11   neuromere   (Fradkin et al., 1995) ventral thoracic disc primordium   (Fradkin et al., 1995) embryonic stage 12   embryonic brain   (Fradkin et al., 1995) neuron of ventral nerve cord   • neuronal cell body (Fradkin et al., 2004) embryonic stage 13   ventral nerve cord | segmentally repeated   (Fradkin et al., 1995) embryonic stage 13 -- 16   anterior commissure   • axon (Fradkin et al., 2004) posterior commissure   • axon (Fradkin et al., 2004) longitudinal connective   • axon (Fradkin et al., 2004)
Additional Descriptive Data
	Wnt55 protein is localized to neuronal cell bodies lateral to the midline of the ventral nerve cord in early embryonic stage 12. At stages embryonic stages 13-16, Wnt5 protein is found on longitudinal axons and on axons of the anterior and posterior commissures, with greatest abundance on the axons of the posterior commissures. (Fradkin et al., 2004) Wnt5 protein expression pattern resembles the Wnt5 transcript expression pattern. At stage 10, expression is detected in bilaterally symmetrical regions corresponding to the labrum and antennal primordia and the labial and maxillary gnathal segments. At stage 11, the protein is found in ventral patches of cells corresponding to the thoracic limb primordia, and in each neuromere. As the germ band retracts, expression is detected in the supraesophageal ganglia of the brain. By stage 13, expression is detected throughout the ventral nerve cord at low levels, with strong staining detected in each hemisegment in a pattern suggestive of the commissural and longitudinal axon bundles. Double staining with MAbBP102, which recognizes all neuronal cells, indicates that Wnt5 protein is expressed in neuronal cells. (Fradkin et al., 1995)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
extracellular region inferred from direct assay (Yoshikawa et al., 2003)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    55.25 Larval Midgut    1.9 Larval Hindgut    15.3 Larval Malpighian Tubules    4 Larval Fat Body    7.1 Larval Salivary Gland    1.1 Larval Trachea    35.025 Larval Carcass    7.4 Adult Head    15.1 Adult Eye    18.25 Adult Brain    41.3 Adult Thoracic-Abdominal Ganglion    39.1 Adult Crop    6.1 Adult Midgut    3.9 Adult Hindgut    7.8 Adult Malpighian Tubules    2.5 Adult Fat Body    6.8 Adult Salivary Gland    6.8 Adult Heart    4.15 Adult VirginFemale Spermatheca    7.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    33.2 Adult Testis    12 Adult Male Accessory Gland    19 Adult Carcass    1.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    18.25   NA  Brain    41.3   55.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    39.1   NA  Crop    6.1   1.9  Midgut    3.9   no informative data  Hindgut    7.8   4  Malpighian Tubules    2.5   7.1  Fat Body    6.8   1.1  Salivary Gland    6.8   NA  Heart    4.15   35.025  Trachea    NA   NA  VirginFemale Spermatheca    7.9   NA  InseminatedFemale Spermatheca    4.9   NA  Ovary    33.2   NA  Testis    no informative data   NA  Male Accessory Gland    no informative data   7.4  Carcass    1.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3  (28) central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3  28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    28 central nervous system, pupae P8    20 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    5 head, mated 20-day female    2 head, mated 1-day male    7 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    7 carcass, larvae L3 wandering    14 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    17 ovary, mated 4-day female    14 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr    33 embryo 06-08hr    49 embryo 08-10hr    42 embryo 10-12hr    41 embryo 12-14hr    38 embryo 14-16hr    26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr  (33) embryo 06-08hr  (49) embryo 08-10hr  (42) embryo 10-12hr  (41) embryo 12-14hr  (38) embryo 14-16hr  26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr    33 embryo 06-08hr    49 embryo 08-10hr    42 embryo 10-12hr    41 embryo 12-14hr    38 embryo 14-16hr    26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr    33 embryo 06-08hr    49 embryo 08-10hr    42 embryo 10-12hr    41 embryo 12-14hr    38 embryo 14-16hr    26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr    33 embryo 06-08hr    49 embryo 08-10hr    42 embryo 10-12hr    41 embryo 12-14hr    38 embryo 14-16hr    26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr  (33) embryo 06-08hr  (49) embryo 08-10hr  (42) embryo 10-12hr  (41) embryo 12-14hr  (38) embryo 14-16hr  26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr    33 embryo 06-08hr    49 embryo 08-10hr    42 embryo 10-12hr    41 embryo 12-14hr    38 embryo 14-16hr    26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    15 embryo 02-04hr    22 embryo 04-06hr    33 embryo 06-08hr    49 embryo 08-10hr    42 embryo 10-12hr    41 embryo 12-14hr    38 embryo 14-16hr    26 embryo 16-18hr    16 embryo 18-20hr    11 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    4 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    12 white prepupae new    15 white prepupae 12hr    13 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    8 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    4 adult female 05day    7 adult female 30day    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3    28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC    30 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3  (28) leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC  (30) ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3    28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC    30 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3    28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC    30 ovary OSS    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3    28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC    30 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3  28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC  30 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3    28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC    30 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    9 embryonic 1182-4H    17 embryonic GM2    19 embryonic Kc167    15 embryonic S1    12 embryonic S3    28 leg disc CME L1    10 wing disc CME-W2    13 wing disc ML-DmD8    15 wing disc ML-DmD9    16 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    11 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    9 CNS ML-DmBG1-c1    11 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    0 ovary fGS/OSS    24 ovary OSC    30 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010194 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6407-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | partially | recessive Wnt5400 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Wnt5GD6521 viable Wnt5D7 Sterility Allele fertile Wnt5D7 Other Phenotypes Allele neuroanatomy defective Wnt5400 Wnt5D7 Wnt5Scer\UAS.cSa neuroanatomy defective, with Scer\GAL4sim.PS Wnt5Scer\UAS.cFa uncoordinated Wnt5D7 visible, with Scer\GAL4pnr-MD237 Wnt5GD6521 Phenotype manifest in Allele antennal lobe Wnt5D7 anterior commissure Wnt5D7 anterior commissure, with Scer\GAL4sim.PS Wnt5EY03178 Wnt5Scer\UAS.cFa axon & dorsal cluster neuron Wnt5400 commissure Wnt5400 fascicle Wnt5400 glomerulus Wnt5D7 intermediate longitudinal fascicle Wnt5400 lateral longitudinal fascicle Wnt5400 lateral transverse muscle | embryonic stage 16 Wnt5400 lateral transverse muscle | third instar larval stage Wnt5400 mesothoracic tergum, with Scer\GAL4pnr-MD237 Wnt5GD6521 MP1 neuron Wnt5400 MP1 tract Wnt5400 presumptive embryonic/larval central nervous system Wnt5400 presumptive embryonic salivary gland Wnt5D7 presumptive embryonic salivary gland, with Scer\GAL4bap.3 Wnt5Scer\UAS.cFa trichogen cell, with Scer\GAL4pnr-MD237 Wnt5GD6521 ventral nerve cord Wnt5Scer\UAS.cSa vMP2 tract Wnt5400
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Wnt5 Class Mutagen Stocks Known lesion Wnt5BG00642 P-element activity 1 Yes Wnt5EY03178 P-element activity 1 Yes Wnt5f04030 piggyBac transposase 1 -- Wnt5GS1192 1 Yes Wnt5207 amorphic allele - genetic evidence P-element activity 0 Yes Wnt5400 amorphic allele - genetic evidence P-element activity 0 Yes Wnt59 P-element activity 0 Yes Wnt5D7 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Wnt5 Class Mutagen Stocks Known lesion Wnt5GD6521 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Wnt5HM05020 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Wnt5HMS01119 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Wnt5KK105240 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Wnt5hs.3x.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Wnt5hs.PF in vitro construct - regulatory fusion 0 Yes Wnt5hs.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Wnt5JF03379 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Wnt5NIG.6407R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Wnt5S868A.Scer\UAS.3x.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - coding region fusion 0 Yes Wnt5Scer\FRT.αTub84B in vitro construct - regulatory fusion 0 Yes Wnt5Scer\UAS.3x.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Wnt5Scer\UAS.cFa in vitro construct - regulatory fusion 0 Yes Wnt5Scer\UAS.cSa in vitro construct - regulatory fusion 0 Yes Wnt5αTub84B.PW FLPase 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)CG6461D84 (Yoshikawa et al., 2003, Yoshikawa et al., 2003) Df(1)E128 (Fradkin et al., 1995, Fradkin et al., 2004) Df(1)fu-E5 (Fradkin et al., 1995) Df(1)N19 (Eisenberg et al., 1992, Fradkin et al., 1995)
Not disrupted in	Df(1)D2 (Fradkin et al., 1995) Df(1)fu-B10 (Fradkin et al., 1995) Df(1)fu-H4 (Fradkin et al., 1995) Df(1)osUE69 (Fradkin et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct M{UAS-Wnt5.3xHA} NA M{UAS-Wnt5.S868A.3xHA} NA P{GD6521} NA P{KK105240} NA P{NIG.6407R} NA P{TRiP.HM05020} NA P{TRiP.HMS01119} NA P{TRiP.JF03379} NA P{UAS-Wnt5.F} NA P{UAS-Wnt5.S} NA heat-shock construct M{hs-Wnt5.3xHA} NA P{hs-Wnt5.F} NA characterization construct P{αTub84B(FRT.wGMR)Wnt5} NA P{αTub84B(-FRT)Wnt5} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Wnt5EY03178 NA P{GSV1}Wnt5GS1192 NA miscellaneous insertions P{(loxP.en.8)w}C6A NA P{GT1}Wnt5BG00642 NA PBac{WH}Wnt5f04030 NA insertion of enhancer trap P{lacW}l(1)G0265G0265 No
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
receptor binding inferred from physical interaction with drl (Yoshikawa et al., 2003)
Biological Process
CV term Evidence References
axon extension inferred from mutant phenotype (Srahna et al., 2006) axon guidance inferred from genetic interaction with drl AND inferred from mutant phenotype (Yoshikawa et al., 2003) inferred from genetic interaction with uzip (Ding et al., 2011) determination of muscle attachment site inferred from mutant phenotype (Lahaye et al., 2012) negative chemotaxis inferred from mutant phenotype (Yoshikawa et al., 2003) salivary gland morphogenesis inferred from mutant phenotype (Harris and Beckendorf, 2007) Wnt receptor signaling pathway inferred from genetic interaction with drl (Yoshikawa et al., 2003)
Cellular Component
CV term Evidence References
extracellular region inferred from direct assay (Yoshikawa et al., 2003)
Terms Based on Predictions or Assertions ( 12 terms )
Molecular Function
CV term Evidence References
frizzled-2 binding inferred from biological aspect of ancestor with PANTHER:PTN000246782 (assigned by RefGenome) (Gaudet et al., 2010) receptor binding inferred from sequence or structural similarity with wg (FlyBase, 1992-)
Biological Process
CV term Evidence References
anterior/posterior axon guidance inferred from biological aspect of ancestor with PANTHER:PTN000246894 (assigned by RefGenome) (Gaudet et al., 2010) anterior/posterior pattern specification inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) canonical Wnt receptor signaling pathway inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) dorsal/ventral axis specification inferred from biological aspect of ancestor with PANTHER:PTN000246782 (assigned by RefGenome) (Gaudet et al., 2010) negative regulation of transcription from RNA polymerase II promoter inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) respiratory system development inferred from biological aspect of ancestor with PANTHER:PTN000246782 (assigned by RefGenome) (Gaudet et al., 2010) Wnt receptor signaling pathway inferred from sequence or structural similarity with wg (FlyBase, 1992-)
Cellular Component
CV term Evidence References
extracellular region inferred from sequence or structural similarity with wg (FlyBase, 1992-) extracellular space inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) plasma membrane inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Wnt5 - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - CG18081 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - CG43444 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - Pdi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - sgl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Wnt5 - sqh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Wnt5 allele Gene References Wnt5D7 drl (Yoshikawa et al., 2003) Wnt5EY03178 drl (Yoshikawa et al., 2003) Wnt5Scer\UAS.cFa por (Fradkin et al., 2004) unspecified dsh (Srahna et al., 2006) fz (Srahna et al., 2006) fz2 (Srahna et al., 2006) pbl (Gregory et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59151 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010194 DroID - A comprehensive database of gene and protein interactions. • FBgn0010194 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32838 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P28466
Orthologs
OrthoDB Orthologs (140) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6MSDHP Dipteran inclusive ortholog search EOG6BVS2R, EOG69ZXZS Insect inclusive ortholog search EOG6NCK7Q Arthropod inclusive ortholog search EOG6T4B9D Metazoa inclusive ortholog search EOG6WX4C7
Orthologs in Drosophila Species (EOG6MSDHP)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Wnt5     Drosophila simulans   Dsim\GD15629 Y   Drosophila sechellia   Dsec\GM22805 Y   Drosophila erecta   Dere\GG18113 Y   Drosophila yakuba   Dyak\GE15518 Y   Drosophila ananassae   Dana\GF13958 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19569 Y   Drosophila persimilis   Dper\GL16238     Drosophila persimilis   Dper\GL16237 Y   Drosophila willistoni   Dwil\GK21945 Y   Drosophila virilis   Dvir\GJ21907     Drosophila virilis   Dvir\GJ21905     Drosophila mojavensis   Dmoj\GI18872 Y   Drosophila grimshawi   Dgri\GH21730 Y   Drosophila grimshawi   Dgri\GH23670
Orthologs in non-Drosophila Dipterans (EOG6BVS2R, EOG69ZXZS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014566   Anopheles gambiae Malaria mosquito  Agam\AGAP010290   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015288
Orthologs in non-Dipteran Insects (EOG6NCK7Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Wnt5   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003720   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21900   Atta cephalotes Leafcutter ant  Acep\ACEP26663   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18440   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19958   Linepithema humile Argentine ant  Lhum\LH13224   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16012   Solenopsis invicta Red fire ant  Sinv\SINV22794   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009172   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003531   Bombyx mori Silkmoth  Bmor\BGIBMGA013783   Pediculus humanus Human body louse  Phum\PHUM038300   Tribolium castaneum Red flour beetle  Tcas\Wnt5
Orthologs in non-Insect Arthropods (EOG6T4B9D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\WNT5   Ixodes scapularis Deer tick  Isca\ISCW020393
Orthologs in non-Arthropod Metazoa (EOG6WX4C7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\mom-2 Y Caenorhabditis elegans Nematode  Cele\wnt-2 Y Caenorhabditis elegans Nematode  Cele\egl-20 Y Caenorhabditis elegans Nematode  Cele\cwn-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013570gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023065gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011756gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004924gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000007gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026277gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001628gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024947gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011130gn Y Danio rerio Zebrafish  Drer\wnt2ba Y Danio rerio Zebrafish  Drer\wnt7a Y Danio rerio Zebrafish  Drer\wnt4a Y Danio rerio Zebrafish  Drer\wnt10b Y Danio rerio Zebrafish  Drer\wnt5a Y Danio rerio Zebrafish  Drer\wnt5b Y Danio rerio Zebrafish  Drer\wnt11r Y Danio rerio Zebrafish  Drer\wnt3a Y Danio rerio Zebrafish  Drer\ENSDARG00000073935 Y Danio rerio Zebrafish  Drer\wnt4b Y Danio rerio Zebrafish  Drer\ENSDARG00000038283 Y Danio rerio Zebrafish  Drer\ENSDARG00000002483 Y Danio rerio Zebrafish  Drer\wnt3 Y Danio rerio Zebrafish  Drer\wnt1 Y Danio rerio Zebrafish  Drer\wnt11 Y Danio rerio Zebrafish  Drer\wnt2bb Y Danio rerio Zebrafish  Drer\wnt2 Y Danio rerio Zebrafish  Drer\wnt7bb Y Danio rerio Zebrafish  Drer\wnt10a Y Danio rerio Zebrafish  Drer\wnt7ba Y Xenopus tropicalis Western clawed frog  Xtro\wnt5b Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000024231 Y Xenopus tropicalis Western clawed frog  Xtro\wnt1 Y Xenopus tropicalis Western clawed frog  Xtro\wnt16 Y Xenopus tropicalis Western clawed frog  Xtro\wnt11 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000007522 Y Xenopus tropicalis Western clawed frog  Xtro\wnt2b Y Xenopus tropicalis Western clawed frog  Xtro\wnt6 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031217 Y Xenopus tropicalis Western clawed frog  Xtro\wnt7c Y Xenopus tropicalis Western clawed frog  Xtro\wnt3a Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031623 Y Xenopus tropicalis Western clawed frog  Xtro\wnt7b Y Xenopus tropicalis Western clawed frog  Xtro\wnt10b Y Xenopus tropicalis Western clawed frog  Xtro\wnt7a Y Xenopus tropicalis Western clawed frog  Xtro\wnt4 Y Xenopus tropicalis Western clawed frog  Xtro\wnt3 Y Xenopus tropicalis Western clawed frog  Xtro\wnt11b Y Gallus gallus Domestic chicken  Ggal\WNT7B Y Gallus gallus Domestic chicken  Ggal\Gga.47682 Y Gallus gallus Domestic chicken  Ggal\WNT4 Y Gallus gallus Domestic chicken  Ggal\WNT2B Y Gallus gallus Domestic chicken  Ggal\WNT16 Y Gallus gallus Domestic chicken  Ggal\WNT11 Y Gallus gallus Domestic chicken  Ggal\WNT7A Y Gallus gallus Domestic chicken  Ggal\WNT5A Y Gallus gallus Domestic chicken  Ggal\WNT5B Y Gallus gallus Domestic chicken  Ggal\WNT3 Y Gallus gallus Domestic chicken  Ggal\WNT6 Y Gallus gallus Domestic chicken  Ggal\WNT10A Y Gallus gallus Domestic chicken  Ggal\WNT2 Y Mus musculus House mouse  Mmus\Wnt7a Y Mus musculus House mouse  Mmus\Wnt5a Y Mus musculus House mouse  Mmus\Wnt2b Y Mus musculus House mouse  Mmus\Wnt1 Y Mus musculus House mouse  Mmus\Wnt3 Y Mus musculus House mouse  Mmus\Wnt11 Y Mus musculus House mouse  Mmus\Wnt5b Y Mus musculus House mouse  Mmus\Wnt3a Y Mus musculus House mouse  Mmus\Wnt10a Y Mus musculus House mouse  Mmus\Wnt4 Y Mus musculus House mouse  Mmus\Wnt6 Y Mus musculus House mouse  Mmus\Wnt7b Y Mus musculus House mouse  Mmus\Wnt10b Y Rattus norvegicus Norway rat  Rnor\Wnt7a Y Rattus norvegicus Norway rat  Rnor\Wnt3 Y Rattus norvegicus Norway rat  Rnor\Wnt2 Y Rattus norvegicus Norway rat  Rnor\Wnt2b Y Rattus norvegicus Norway rat  Rnor\Wnt16 Y Rattus norvegicus Norway rat  Rnor\Wnt1 Y Rattus norvegicus Norway rat  Rnor\Wnt3a Y Rattus norvegicus Norway rat  Rnor\Wnt6 Y Rattus norvegicus Norway rat  Rnor\Wnt10b Y Rattus norvegicus Norway rat  Rnor\Wnt5a Y Rattus norvegicus Norway rat  Rnor\Wnt10a Y Rattus norvegicus Norway rat  Rnor\Wnt5b Y Rattus norvegicus Norway rat  Rnor\Wnt4 Y Rattus norvegicus Norway rat  Rnor\Wnt7b Y Rattus norvegicus Norway rat  Rnor\Wnt11 Y Homo sapiens Human  Hsap\WNT5A Y Homo sapiens Human  Hsap\WNT11 Y Homo sapiens Human  Hsap\WNT2B Y Homo sapiens Human  Hsap\WNT3 Y Homo sapiens Human  Hsap\WNT7B Y Homo sapiens Human  Hsap\WNT4 Y Homo sapiens Human  Hsap\WNT2 Y Homo sapiens Human  Hsap\WNT6 Y Homo sapiens Human  Hsap\WNT3A Y Homo sapiens Human  Hsap\WNT10B Y Homo sapiens Human  Hsap\WNT1 Y Homo sapiens Human  Hsap\WNT10A Y Homo sapiens Human  Hsap\WNT7A Y Homo sapiens Human  Hsap\WNT16 Y
Human Orthologs (14)
Gene OMIM HGNC Hsap\WNT5A     Hsap\WNT11     Hsap\WNT2B     Hsap\WNT3     Hsap\WNT7B     Hsap\WNT4     Hsap\WNT2     Hsap\WNT6     Hsap\WNT3A     Hsap\WNT10B     Hsap\WNT1     Hsap\WNT10A     Hsap\WNT7A     Hsap\WNT16
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15629 Drosophila sechellia Dsec\GM22805 Drosophila erecta Dere\GG18113 Drosophila yakuba Dyak\GE15518 Drosophila ananassae Dana\GF13958 Drosophila pseudoobscura pseudoobscura Dpse\GA19569 Drosophila persimilis Dper\GL16237 Drosophila willistoni Dwil\GK21945 Drosophila mojavensis Dmoj\GI18872 Drosophila grimshawi Dgri\GH21730
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	12428 w1118 P{GT1}Wnt5BG00642 28534 y1 v1; P{TRiP.HM05020}attP2 19833 y1 w67c23 P{EPgy2}Wnt5EY03178 34644 y1 sc* v1; P{TRiP.HMS01119}attP2
Harvard	- PBac{WH}Wnt5f04030
Kyoto	200177 y1 w67c23 P{GSV1}Wnt5GS1192/Binsinscy
VDRC	v101621 P{KK105240}VIE-260B v32257 w1118; P{GD6521}v32257 v30863 w1118; P{GD6521}v30863/TM3
Genomic Clones ( 1 )
	BACR03K10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 50 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD28671 RE25179
Other clones	DPiM_RE25179
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD22778 LD27626 LD30719 LD32139 LD35976 RE01039 RE05942 RE24307 RE38448 RE38548 RE46476 RE49691 RE50336 RE64147 RE65673 RE65873
Other clones	11361 18551 52932 77314 77367 81802 83987 108602 148298 161721 557038 569484 CK01.21C.F10 CK01.142B.G3 EK019841 EK053705 EK111939 EK118926 EK130563 EK168955 EK210634 EK210938 EK215227 EK218416 EK235162 EK266031 EK272105 EN11918 EN14960 LD22614 RP003186452
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010194 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32838
Antibody Information
	polyclonal (Fradkin et al., 1995, Shimizu et al., 2011) polyclonal antibody (Fradkin et al., 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Wnt5 CG6407 (Li, 1999.11)
Source for database merge of	Source for merge of: Wnt5 anon-WO03040301.228 (FlyBase, 1996-)
Additional comments	"l(1)G0265" may affect "Wnt5". (Peter et al., 2002) Source for merge of Wnt5 anon-WO03040301.228 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	drl and Wnt5 are required (in the salivary gland and in the central nervous system respectively) for normal salivary gland migration in the embryo. They are required in the third phase of migration, to mediate the final positioning of the gland, as the salivary gland detaches from the circular visceral mesoderm and contacts the longitudinal visceral mesoderm. (Harris and Beckendorf, 2007) Wnt5 has a role in axon guidance as a ligand for drl. (Yoshikawa et al., 2003) Wnt5 cannot functionally replace wg in an in vivo assay. Transfection of cell culture with a construct encoding Wnt5 demonstrates Wnt5 to be a secreted protein which is likely to play a role in development of the CNS. (Fradkin et al., 1995) Wnt3 and Wnt5 are the same gene, and as the sequence is more similar to the vertebrate Wnt-5a and Wnt-5b genes the Wnt5 designation is more appropriate. (Eisenberg et al., 1992) The expression of Wnt5 depends on the activity of Dll. (Eisenberg et al., 1992) Wnt5 encodes an amino terminal signal peptide suggesting the gene product is a secreted protein. The distribution of Wnt5 transcripts in cells of the CNS during embryogenesis suggests Wnt5 plays a role in CNS development. (Russell et al., 1992) Sequence analysis of Wnt genes is performed in several species to determine the ancestral lineage of the gene family. (Sidow, 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057576 RefSeq (Proteins) • NP_476924 DDBJ / EMBL / GenBank • AAA29020 AAF48853 AAQ23586 ABA86448 AC012098 AX777374 AZ877648 BI231990 BT010268 CAA46002 CZ486618 DQ138842 M97450 X64736 Entrez Gene - A searchable database of RefSeq genes. • 32838 UniProtKB/Swiss-Prot • P28466 UniProtKB/TrEMBL • Q2XY20
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Wnt protein, conserved site (IPR018161) Wnt (IPR005817)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59151 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010194 DroID - A comprehensive database of gene and protein interactions. • FBgn0010194 DRSC - Results from RNAi screens. • FBgn0010194 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010194 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6407-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010194 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32838 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/wnt5-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32838 modMine - Data generated by the modENCODE project. • FBgn0010194 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P28466
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	anon-WO03040301.228   CG6407 (Keleman et al., 2009.8.5, Perkins et al., 2009, Muse et al., 2007, Ni et al., 2010.12.1) Dm-3 (Sidow, 1992) Dm DWnt3/5 (Konikoff et al., 2010) DWnt3 (Wu and Nusse, 2002, Llimargas and Lawrence, 2001, Reifegerste and Moses, 1999) DWnt-3 (Nusse, 1992.3.2, Fradkin et al., 1995, Graba et al., 1995, Siegfried and Perrimon, 1994, Russell et al., 1992) Dwnt-3 (Endo et al., 1997, Karlin and Burge, 1996, Thaker and Nusse, 1995, ) DWnt-3/5 (Tanaka et al., 2002, Cadigan and Nusse, 1997) DWnt3/5 (Coudreuse and Korswagen, 2007, Port et al., 2008) DWnt5 (Bajpai et al., 2004, Llimargas and Lawrence, 2001, Chen et al., 2008, Port et al., 2008) Dwnt5 (Panganiban and Rubenstein, 2002, Eisenberg et al., 1992) DWnt-5 (Mourkioti et al., 2001) Dwnt-5 (Eisenberg, 1992.7.27, ) Wnt (Blair, 1999) Wnt-3 (Jockusch and Ober, 2000, Wehrli and Tomlinson, 1998) Wnt3 (Taylor et al., 1998, Casal et al., 2006) wnt3   Wnt3/5 (Schubert et al., 2000) wnt5 (Charron, 2005, Veeman et al., 2003, Yoshikawa and Thomas, 2004, Liebl et al., 2008, Srahna et al., 2006, Yao et al., 2007, Fradkin et al., 2004, Zlatic et al., 2009, Glise et al., 2005, Ding et al., 2011) Wnt5 (Chilton, 2006, Gregory et al., 2007, Nechaev et al., 2010, Gordon and Nusse, 2006, Bovolenta et al., 2006, Herr and Basler, 2012, Casal et al., 2006, Harris and Beckendorf, 2007, Ching et al., 2008, Shimizu et al., 2011, Grillenzoni et al., 2007, Wouda et al., 2008, Sakurai et al., 2009, Zeitlinger et al., 2007, Muse et al., 2007, Singh et al., 2010, Lahaye et al., 2012, Japanese National Institute of Genetics, 2012.5.21)
Name Synonym	wnt-oncogene-analog-3   Wnt oncogene analog 5
Secondary FlyBase IDs
	FBgn0004361 FBgn0066822
References ( 98 )
Generate a list of	All references relating to this gene ( 98 )
List References by type	Research paper  ( 42 ) Supplementary material  ( 6 ) Review  ( 20 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 7 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 5 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 ) Patent  ( 1 ) Protein sequence record  ( 1 )
Recent research papers ( 5 )
	Herr and Basler, 2012, Dev. Biol. 361(2): 392--402 Porcupine-mediated lipidation is required for Wnt recognition by Wls. [FBrf0217047] Lahaye et al., 2012, PLoS ONE 7(3): e32297 WNT5 Interacts with the Ryk Receptors Doughnut and Derailed to Mediate Muscle Attachment Site Selection in Drosophila melanogaster. [FBrf0217725] Ding et al., 2011, Dev. Dyn. 240(1): 122--134 Glial cell adhesive molecule unzipped mediates axon guidance in Drosophila. [FBrf0212620] Noyes et al., 2011, G3 (Bethesda) 1(6): 471--478 Modulation of the activity of a polycomb-group response element in Drosophila by a mutation in the transcriptional activator woc. [FBrf0217652] Shimizu et al., 2011, J. Neurosci. 31(13): 4944--4954 The wnt5/planar cell polarity pathway regulates axonal development of the Drosophila mushroom body neuron. [FBrf0213382] Show all research papers ...
Recent reviews ( 1 )
	Broadie et al., 2011, Dev. Neurobiol. 71(11): 1102--1130 Extracellular matrix and its receptors in drosophila neural development. [FBrf0216377] Show all reviews ...