FlyBase Gene Report: Dmel\RpS15Aa

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\RpS15Aa or the JBrowse view of Dmel\RpS15Aa for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS15Aa-RA

FBtr0073793

NM_167360

678

130

RpS15Aa-RC

FBtr0073792

NM_167362

737

130

RpS15Aa-RD

FBtr0073794

NM_079970

877

130

RpS15Aa-RE

FBtr0073795

NM_167363

741

130

RpS15Aa-RF

FBtr0300828

NM_001169267

674

130

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

RpS15Aa-PA

FBpp0073624

14.8

130

10.48

NP_727690

AAF48256

RpS15Aa-PC

FBpp0073623

14.8

130

10.48

NP_727692

AAN09322

RpS15Aa-PD

FBpp0073625

14.8

130

10.48

NP_524709

AAF48257

RpS15Aa-PE

FBpp0073626

14.8

130

10.48

NP_727693

AAN09323

RpS15Aa-PF

FBpp0290052

14.8

130

10.48

NP_001162738

ACZ95273

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

130 aa isoforms: RpS15Aa-PA, RpS15Aa-PC, RpS15Aa-PD, RpS15Aa-PE, RpS15Aa-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS15Aa using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS15Aa_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

structural constituent of ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

structural constituent of ribosome

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000204723

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

cytoplasmic translation

inferred by curator from GO:0022626

(Alonso and Santaren, 2006)

traceable author statement

(Marygold et al., 2007)

translation

inferred from electronic annotation with InterPro:IPR000630

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

cytosolic ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

cytosolic small ribosomal subunit

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000204723

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

cytosolic ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS15Aa in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS15Aa

View exonic expression by tissue for Dmel\RpS15Aa

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010198

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010198

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12324-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2033

Flygut - An atlas of the Drosophila adult midgut

FBgn0010198

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS15Aa

Transgenic constructs containing regulatory region of RpS15Aa

Deletions and Duplications ( 9 )

Disrupted in

Df(1)BSC764

(Christensen et al., 2009.2.28)

Df(1)BSC765

(Christensen et al., 2009.2.28)

Df(1)BSC766

(Christensen et al., 2009.2.28)

Df(1)BSC767

(Christensen et al., 2009.2.28)

Df(1)C246

(Marygold et al., 2007)

Df(1)ED7173

(Marygold et al., 2007)

Df(1)Exel6245

(Marygold et al., 2007, Ryder, 2004.4.26)

Df(1)f08066-f07791

(Casad et al., 2011)

Df(1)N12

(Marygold et al., 2007)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

RpS15Aa^GD8827

lethal, with Scer\GAL4^pnr-MD237

RpS15Aa^GD8827

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

partially lethal - majority die, with Scer\GAL4^tin.CΔ4

RpS15Aa^GD8827

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpS15Aa^GD8827

Other Phenotypes

Allele

size defective | adult stage, with Scer\GAL4^tin.CΔ4

RpS15Aa^GD8827

size defective | adult stage, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

RpS15Aa^GD8827

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^tin.CΔ4

RpS15Aa^GD8827

adult heart, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

RpS15Aa^GD8827

embryonic/larval somatic muscle, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

sarcomere, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

Z disc, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RPS15A

13 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rps15a

12 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Rps15a

12 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\rps15a

8 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\rps15a

13 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\rps-22

12 of 15

Yes

Arabidopsis thaliana (thale-cress) (6)

Atha\AT2G39590

9 of 9

Yes

Atha\RPS15AD

8 of 9

Yes

Atha\RPS15A

7 of 9

Yes

Atha\rps15ab

3 of 9

Yes

Atha\rps15ae

3 of 9

Yes

Atha\AT5G59850

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\RPS22A

14 of 15

Yes

Scer\RPS22B

14 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rps2202

11 of 12

Yes

Spom\rps2201

5 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190I9V )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\RpS15Aa

Drosophila melanogaster

fruit fly

Dmel\RpS15Ab

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15195

Drosophila persimilis

Dper\GL26726

Drosophila willistoni

Dwil\GK16027

Drosophila virilis

Dvir\GJ15828

Drosophila mojavensis

Dmoj\GI16425

Drosophila grimshawi

Dgri\GH17815

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EGW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010998

Glossina morsitans

Tsetse fly

Gmor\GMOY008583

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04916

Mayetiola destructor

Hessian fly

Mdes\Mdes014709

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009151

Anopheles darlingi

American malaria mosquito

Adar\ADAC007484

Anopheles gambiae

Malaria mosquito

Agam\Rps15a-1

Anopheles gambiae

Malaria mosquito

Agam\Rps15a-2

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006671

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0HZ2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\RpS15A

Danaus plexippus

Monarch butterfly

Dple\DPOGS206085-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL012111

Apis florea

Little honeybee

Aflo\2183457476

Apis mellifera

Western honey bee

Amel\LOC551125

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25055

Bombus terrestris

Buff-tailed bumblebee

Bter\15204992

Linepithema humile

Argentine ant

Lhum\LH10396

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00166f

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004922

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08007

Tribolium castaneum

Red flour beetle

Tcas\TC012128

Pediculus humanus

Human body louse

Phum\PHUM023290

Rhodnius prolixus

Kissing bug

Rpro\RPRC007230

Cimex lectularius

Bed bug

Clec\CLEC003912

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000930

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01819

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0I21 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR002571

Ixodes scapularis

Black-legged tick

Isca\ISCW015484

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65019.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g06460

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302797

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0TSE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rps15a

Ciona intestinalis

Vase tunicate

Cint\LOC100180346

Gallus gallus

Domestic chicken

Ggal\RPS15A

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

9 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS15A; ribosomal protein S15a

13 of 15

603674

DIAMOND-BLACKFAN ANEMIA 20; DBA20

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS15Aa - Akt1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Lkb1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Pi3K92E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpS15Aa - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL37a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Thor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - chico

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS15Aa - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68867

DroID - A comprehensive database of gene and protein interactions.

FBgn0010198

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44150

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS15Aa

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-42

Cytogenetic map

11E11-11E12

Sequence location

X:13,335,637..13,337,705 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11E11-11E12

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-42

(FlyBase, 2016)

1-42.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Bloomington

34005

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00968}attP2

FlyORF

F000765

M{UAS-RpS15Aa.ORF.3xHA}ZH-86Fb

VDRC

v19198

w¹¹¹⁸; P{GD8827}v19198

v50344

w¹¹¹⁸ P{GD17481}v50344

v50635

w¹¹¹⁸; P{GD17481}v50635

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (355)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010198

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010198

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44150

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: RpS15A CG2033

(Whitfield, 2000.10.25, Gong, 1999.11)

Source for database merge of

Additional comments

The RpS15Aa and RpS15Ab genes share at least 50% identity.

(Langille and Clark, 2007)

"RpS15Aa" likely corresponds to "M(1)11F".

(Marygold et al., 2007)

The RpS15Ab gene may have been derived from the RpS15Aa gene by retroposition.

(Dai et al., 2006)

The RpS15Ab gene may be derived from the RpS15Aa gene by retroposition.

(Betran et al., 2002)

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Likely Minute gene.

(Marygold et al., 2007)

Deletions removing RpS15Aa but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

(Marygold et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Isolated from a Drosophila cDNA expression library in a screen for clones able to rescue the S.cerevisiae cdc33ts4-2 mutant.

(Lavoie et al., 1994)

RpS15Aa has been cloned and sequenced.

(Lavoie et al., 1994)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44150

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P48149

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JJM9

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010198

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010198

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS15Aa_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2033

Flygut - An atlas of the Drosophila adult midgut

FBgn0010198

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44150

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2033

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

modMine - A data warehouse for the modENCODE project

FBgn0010198

Linkouts

BioGRID - A database of protein and genetic interactions.

68867

DroID - A comprehensive database of gene and protein interactions.

FBgn0010198

DRSC - Results frm RNAi screens

FBgn0010198

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010198

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12324-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0010198

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44150

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS15Aa

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Rp S15 Aa

(Alonso and Santaren, 2006)

RpS15A

(Cermelli et al., 2006, Betran et al., 2002, Lavoie et al., 1994)

RpS15a

(Majzoub et al., 2014)

S15

(Brogna et al., 2002)

S15Aa

(Carpenter et al., 2009)

anon-EST:Posey44

Name Synonyms

Ribosomal protein S15A

Ribosomal protein S15Aa

(Jaspers et al., 2012, Cammarato et al., 2011, Ashburner and Marygold, 2004.7.2)

Secondary FlyBase IDs

FBgn0025291
FBgn0030473

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (72)

Report Sections

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