FlyBase Gene Report: Dmel\RpS15Aa

General Information

Symbol

Dmel\RpS15Aa

Species

D. melanogaster

Name

Ribosomal protein S15Aa

Annotation Symbol

CG2033

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010198

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

RpS15A

Key Links

Genomic Location

Cytogenetic map

11E11-11E12

Sequence location

X:13,335,637..13,337,705 [-]

Recombination map

1-42

RefSeq locus

NC_004354 REGION:13335637..13337705

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from direct assay

(Anger et al., 2013)

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from biological aspect of ancestor with PANTHER:PTN000204723

(GO Reference Genome Project, 2011-)

traceable author statement

(Marygold et al., 2007)

inferred from electronic annotation with InterPro:IPR000630, InterPro:IPR035987

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

inferred by curator from GO:0022626

(Anger et al., 2013, Alonso and Santaren, 2006)

traceable author statement

(Marygold et al., 2007)

involved_in translation

inferred from electronic annotation with InterPro:IPR000630, InterPro:IPR035987

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of cytosolic small ribosomal subunit

inferred from biological aspect of ancestor with PANTHER:PTN000204723

(GO Reference Genome Project, 2011-)

traceable author statement

(Marygold et al., 2007)

located_in ribosome

inferred from electronic annotation with InterPro:IPR000630, InterPro:IPR035987

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS

Protein Family (UniProt)

Belongs to the universal ribosomal protein uS8 family. (P48149)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpS15Aa for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P48149)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS15Aa-RA

FBtr0073793

NM_167360

678

130

RpS15Aa-RC

FBtr0073792

NM_167362

737

130

RpS15Aa-RD

FBtr0073794

NM_079970

877

130

RpS15Aa-RE

FBtr0073795

NM_167363

741

130

RpS15Aa-RF

FBtr0300828

NM_001169267

674

130

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RpS15Aa-PA

FBpp0073624

14.8

130

10.48

FBpp0073623

14.8

130

10.48

FBpp0073625

14.8

130

10.48

FBpp0073626

14.8

130

10.48

FBpp0290052

14.8

130

10.48

P48149

NP_001162738

ACZ95273

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

130 aa isoforms: RpS15Aa-PA, RpS15Aa-PC, RpS15Aa-PD, RpS15Aa-PE, RpS15Aa-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS15Aa using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS15Aa_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.29

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RpS15Aa in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS15Aa

View exonic expression by tissue for Dmel\RpS15Aa

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010198

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44150/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010198

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010198

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010198

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2033

Flygut - An atlas of the Drosophila adult midgut

FBgn0010198

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS15Aa

Transgenic constructs containing regulatory region of RpS15Aa

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(1)BSC764

(Christensen et al., 2009.2.28)

Df(1)BSC765

(Christensen et al., 2009.2.28)

Df(1)BSC766

(Christensen et al., 2009.2.28)

Df(1)BSC767

(Christensen et al., 2009.2.28)

Df(1)C246

(Marygold et al., 2007)

Df(1)ED7173

(Marygold et al., 2007)

Df(1)Exel6245

(Marygold et al., 2007, Ryder, 2004.4.26)

Df(1)f08066-f07791

(Casad et al., 2011)

Df(1)N12

(Marygold et al., 2007)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

RpS15Aa^GD8827

lethal, with Scer\GAL4^pnr-MD237

RpS15Aa^GD8827

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

partially lethal - majority die, with Scer\GAL4^tin.CΔ4

RpS15Aa^GD8827

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpS15Aa^GD8827

Other Phenotypes

Allele

abnormal size | adult stage, with Scer\GAL4^tin.CΔ4

RpS15Aa^GD8827

abnormal size | adult stage, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

RpS15Aa^GD8827

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^tin.CΔ4

RpS15Aa^GD8827

adult heart, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

RpS15Aa^GD8827

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

sarcomere, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

Z disc, with Scer\GAL4^Mef2.PR

RpS15Aa^GD8827

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\RPS15A

14 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (8)

Rnor\Rps15a

14 of 14

Yes

Rnor\LOC691716

11 of 14

Yes

Rnor\LOC103693101

11 of 14

Yes

Rnor\Rps15al2

11 of 14

Yes

Rnor\Rps15al4

11 of 14

Yes

Rnor\Fndc10

9 of 14

Yes

Rnor\LOC680996

8 of 14

Yes

Rnor\LOC100364191

2 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Rps15a

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\rps15a

11 of 13

Yes

Xtro\dhx8

1 of 13

Danio rerio (Zebrafish) (1)

Drer\rps15a

14 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\rps-22

13 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP009572

12 of 12

Yes

Agam\AgaP_AGAP000541

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (7)

Atha\AT2G39590

13 of 13

Yes

Atha\AT5G59850

12 of 13

Yes

Atha\RPS15A

12 of 13

Yes

Atha\RPS15AD

12 of 13

Yes

Atha\rps15ab

5 of 13

Yes

Atha\rps15ae

5 of 13

Yes

Atha\rps8

2 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\RPS22A

13 of 13

Yes

Scer\RPS22B

13 of 13

Yes

Scer\MRPS8

3 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\rps2201

12 of 12

Yes

Spom\rps2202

12 of 12

Yes

Spom\mrps8

3 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\rpsH

7 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RpS15Aa. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

RpS15Ab

13 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

cardiomyopathy (postulated), translation-critical genes

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

RpS15Aa^GD8827

model of intrinsic cardiomyopathy

CEA

(Casad et al., 2011)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

RPS15A; ribosomal protein S15a

model of Diamond-Blackfan anemia 20

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS15A; ribosomal protein S15a

14 of 14

603674

DIAMOND-BLACKFAN ANEMIA 20; DBA20

Diamond-Blackfan anemia 20

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS15Aa - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Lkb1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Pi3K92E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

RpS15Aa - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL37-1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - Thor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - chico

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - clu

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(Sen et al., 2024)

RpS15Aa - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS15Aa - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS15Aa - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS15Aa - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS15Aa - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68867

DroID - A comprehensive database of gene and protein interactions.

FBgn0010198

MIST (protein-protein) - An integrated Molecular Interaction Database

44150

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-42

Cytogenetic map

11E11-11E12

Sequence location

X:13,335,637..13,337,705 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11E11-11E12

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-42

(FlyBase, 2016)

1-42.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

34005

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00968}attP2

FlyORF

F000765

M{UAS-RpS15Aa.ORF.3xHA}ZH-86Fb

NIG-Fly

HMS00968

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00968}attP2/TM3, Sb¹

VDRC

v19198

w¹¹¹⁸; P{GD8827}v19198

v50635

w¹¹¹⁸; P{GD17481}v50635

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (343)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE54483

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010198

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010198

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Likely Minute gene.

(Marygold et al., 2007)

Deletions removing RpS15Aa but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

(Marygold et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Isolated from a Drosophila cDNA expression library in a screen for clones able to rescue the S.cerevisiae cdc33ts4-2 mutant.

(Lavoie et al., 1994)

RpS15Aa has been cloned and sequenced.

(Lavoie et al., 1994)

Relationship to Other Genes

Source for database merge of

Additional comments

The RpS15Aa and RpS15Ab genes share at least 50% identity.

(Langille and Clark, 2007)

"RpS15Aa" likely corresponds to "M(1)11F".

(Marygold et al., 2007)

The RpS15Ab gene may have been derived from the RpS15Aa gene by retroposition.

(Dai et al., 2006)

The RpS15Ab gene may be derived from the RpS15Aa gene by retroposition.

(Betran et al., 2002)

Nomenclature History

Source for database identify of

Source for identity of: RpS15A CG2033

(Whitfield, 2000.10.25, Gong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

Rp S15 Aa

(Alonso and Santaren, 2006)

RpS15A

(Cermelli et al., 2006, Betran et al., 2002, Lavoie et al., 1994)

RpS15a

(Majzoub et al., 2014)

S15

(Brogna et al., 2002)

S15Aa

(Carpenter et al., 2009)

(Anger et al., 2013)

anon-EST:Posey44

Name Synonyms

Ribosomal protein S15A

Ribosomal protein S15Aa

(Jaspers et al., 2012, Cammarato et al., 2011, Ashburner and Marygold, 2004.7.2)

Secondary FlyBase IDs

FBgn0025291
FBgn0030473

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44150

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P48149

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P48149

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JJM9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P48149

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010198

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44150/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010198

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010198

FlyMine - An integrated database for Drosophila genomics

FBgn0010198

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2033

MARRVEL_MODEL - MARRVEL (model organism gene)

44150

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

68867

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010198

DroID - A comprehensive database of gene and protein interactions.

FBgn0010198

DRSC - Results frm RNAi screens

FBgn0010198

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS15Aa_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010198

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010198

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2033

Flygut - An atlas of the Drosophila adult midgut

FBgn0010198

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0010198

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

44150

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (83)

The following external sites may use different assemblies or annotations than FlyBase.

-1.29

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