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Gene Dmel\Dbp45A

General Information
Symbol	Dmel\Dbp45A	Species	D. melanogaster
Name	DEAD box protein 45A	Annotation symbol	CG12759
Feature type	protein_coding_gene	FlyBase ID	FBgn0010220
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	45A1-45A2	Sequence location	2R:4,975,339..4,977,307 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene DEAD box protein 45A is referred to in FlyBase by the symbol Dmel\Dbp45A (CG12759, FBgn0010220). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATP-dependent RNA helicase activity. There is experimental evidence that it is involved in the biological process: regulation of alternative mRNA splicing, via spliceosome. 4 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; Helicase, superfamily 1/2, ATP-binding domain; RNA helicase, ATP-dependent, DEAD-box, conserved site; RNA helicase, DEAD-box type, Q motif. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval trachea, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1625634_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Dbp45A. Gene sequence location is 2R:4975339..4977307.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45A1-45A2
Experimentally Determined Cytological Location
Cytogenetic map Notes References 45A-45A     (Lavoie, 1993) 45A-45A   (determined by in situ hybridisation)   (Lavoie et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Dbp45A for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Dbp45A-RA FBtr0088564  NM_057579   1670   521
Additional Transcript Data & Comments
Reported size (kB)	1.8 (northern blot) (Lavoie et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Dbp45A-PA   FBpp0087645   59.7   521   9.40     NP_476927   AAF58994
Additional Polypeptide Data & Comments
Reported size (kDa)	527 (aa); 60 (kD predicted) (Lavoie et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA helicase, ATP-dependent, DEAD-box, conserved site (IPR000629) Helicase, C-terminal (IPR001650) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) RNA helicase, DEAD-box type, Q motif (IPR014014) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA439210   AA540379   AA735226   AA942019   AE013599 AAF58994   AC005458   AC006074   AI514519   AI515613   AW943909   AY058728 AAL13957   BG640710   BI354840   BI637256   CO264495   CO294404   CO305512   CO311334   EC051105   EC051617   EC054109   EC056170   EC086884   EC232777   EC247939   EC249270   GH941167   GH944398   GH945567   GH945822   GH946405   GH947309   GH947779   GH947787   GH948129   GH948173   GH948518   GH948597   GH951134   GH951363   GH952482   GH953621   GH953658   GH954286   GH955442   GH955761   GH958009   GH960152   GH960265   GH964076   GH964435   GH965008   GH965422   GH967000   GH967036   GH967554   GH967793   GH969210   GH969237   GH969680   GH971105   GH971371   GH972663   GH973296   GH973329   GH973870   GH978016   GH978359   GH981079   GH981423   GH982321   GH982887   GH983455   GH984247   GH984484   GH984858   L13612 AAA16339   Z23266 CAA80804
UniProtKB/Swiss-Prot	Q07886
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Lavoie et al., 1993)
Additional Descriptive Data
	Dbp45A transcripts are detected throughout development on northern blots. They are most abundant in 6-12hr embryos and in adult females and least abundant in pupae. (Lavoie et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval trachea, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    135.35 Larval Midgut    44 Larval Hindgut    78.1 Larval Malpighian Tubules    51 Larval Fat Body    45.2 Larval Salivary Gland    71.5 Larval Trachea    134.775 Larval Carcass    85.675 Adult Head    77.5 Adult Eye    106.375 Adult Brain    103.1 Adult Thoracic-Abdominal Ganglion    81.9 Adult Crop    92.1 Adult Midgut    56.9 Adult Hindgut    68.9 Adult Malpighian Tubules    64.7 Adult Fat Body    83.1 Adult Salivary Gland    89.4 Adult Heart    64.75 Adult VirginFemale Spermatheca    93.9 Adult InseminatedFemale Spermatheca    97.7 Adult Ovary    124.5 Adult Testis    45.3 Adult Male Accessory Gland    199.5 Adult Carcass    59.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    77.5   NA  Eye    106.375   NA  Brain    103.1   135.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    81.9   NA  Crop    92.1   no informative data  Midgut    56.9   78.1  Hindgut    68.9   51  Malpighian Tubules    64.7   no informative data  Fat Body    83.1   71.5  Salivary Gland    89.4   NA  Heart    64.75   134.775  Trachea    NA   NA  VirginFemale Spermatheca    93.9   NA  InseminatedFemale Spermatheca    97.7   NA  Ovary    124.5   NA  Testis    no informative data   NA  Male Accessory Gland    199.5   85.675  Carcass    59.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    12 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    8 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    17 ovary, mated 4-day female    18 testis, mated 4-day male    10 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr    30 embryo 06-08hr    31 embryo 08-10hr    31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr  (30) embryo 06-08hr  (31) embryo 08-10hr  (31) embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr    30 embryo 06-08hr    31 embryo 08-10hr    31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr    30 embryo 06-08hr    31 embryo 08-10hr    31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr    30 embryo 06-08hr    31 embryo 08-10hr    31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr  30 embryo 06-08hr  31 embryo 08-10hr  31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr    30 embryo 06-08hr    31 embryo 08-10hr    31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    22 embryo 04-06hr    30 embryo 06-08hr    31 embryo 08-10hr    31 embryo 10-12hr    24 embryo 12-14hr    19 embryo 14-16hr    13 embryo 16-18hr    11 embryo 18-20hr    10 embryo 20-22hr    20 embryo 22-24hr    21 larva L1    22 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    6 adult male 05day    8 adult male 30day    7 adult female 01day    12 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2    29 embryonic Kc167    39 embryonic S1    20 embryonic S3    30 leg disc CME L1    28 wing disc CME-W2    26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2  (29) embryonic Kc167  (39) embryonic S1    20 embryonic S3  (30) leg disc CME L1  (28) wing disc CME-W2  26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5  26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (32) tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2    29 embryonic Kc167    39 embryonic S1    20 embryonic S3    30 leg disc CME L1    28 wing disc CME-W2    26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2    29 embryonic Kc167    39 embryonic S1    20 embryonic S3    30 leg disc CME L1    28 wing disc CME-W2    26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2    29 embryonic Kc167    39 embryonic S1    20 embryonic S3    30 leg disc CME L1    28 wing disc CME-W2    26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2  29 embryonic Kc167  (39) embryonic S1    20 embryonic S3  30 leg disc CME L1  28 wing disc CME-W2  26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5  26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2    29 embryonic Kc167    39 embryonic S1    20 embryonic S3    30 leg disc CME L1    28 wing disc CME-W2    26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    22 embryonic 1182-4H    23 embryonic GM2    29 embryonic Kc167    39 embryonic S1    20 embryonic S3    30 leg disc CME L1    28 wing disc CME-W2    26 wing disc ML-DmD8    17 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    15 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    15 ovary fGS/OSS    17 ovary OSC    19 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    17 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_08 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD47509 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010220 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12759-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Dbp45AGD7822 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 Dbp45AGD7822 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 Dbp45AGD7822 trichogen cell, with Scer\GAL4pnr-MD237 Dbp45AGD7822
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Dbp45A Class Mutagen Stocks Known lesion Dbp45ANP7328 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Dbp45A Class Mutagen Stocks Known lesion Dbp45AGD7822 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Dbp45AKK101176 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Dbp45ANIG.12759R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7822} NA P{KK101176} NA P{NIG.12759R} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system P{GawB}Dbp45ANP7328 No
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
regulation of alternative mRNA splicing, via spliceosome inferred from mutant phenotype (Park et al., 2004)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000629, InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) ATP-dependent RNA helicase activity inferred from sequence or structural similarity with Saccharomyces DBP8 (Lasko, 2000) non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) nucleic acid binding inferred from electronic annotation with InterPro:IPR000629, InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0000381 (Park et al., 2004)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Dbp45A - CG14141 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - CG5645 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - CG8323 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - Kr-h1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - Nf-YB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - Opbp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - Sk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - ihog anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Dbp45A - tor anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Dbp45A allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0010220 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35917
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HQF41 Dipteran inclusive ortholog search EOG6J11HG Insect inclusive ortholog search EOG66Q5P1 Arthropod inclusive ortholog search EOG66Q580 Metazoa inclusive ortholog search EOG63C0G3
Orthologs in Drosophila Species (EOG6HQF41)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Dbp45A     Drosophila simulans   Dsim\GD10627 Y   Drosophila sechellia   Dsec\GM21090 Y   Drosophila erecta   Dere\GG23409 Y   Drosophila yakuba   Dyak\GE19250 Y   Drosophila ananassae   Dana\GF12227 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11795 Y   Drosophila persimilis   Dper\GL17616 Y   Drosophila willistoni   Dwil\GK23245 Y   Drosophila virilis   Dvir\GJ21163 Y   Drosophila mojavensis   Dmoj\GI20069 Y   Drosophila grimshawi   Dgri\GH21617 Y
Orthologs in non-Drosophila Dipterans (EOG6J11HG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014904   Anopheles gambiae Malaria mosquito  Agam\AGAP005130   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008983
Orthologs in non-Dipteran Insects (EOG66Q5P1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC725441   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009633   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11086   Atta cephalotes Leafcutter ant  Acep\ACEP19946   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15740   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19760   Linepithema humile Argentine ant  Lhum\LH17932   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20661   Solenopsis invicta Red fire ant  Sinv\SINV23319   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008863   Bombyx mori Silkmoth  Bmor\BGIBMGA009286   Pediculus humanus Human body louse  Phum\PHUM061090   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07723
Orthologs in non-Insect Arthropods (EOG66Q580)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_213372
Orthologs in non-Arthropod Metazoa (EOG63C0G3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\H20J04.4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018841gn   Danio rerio Zebrafish  Drer\ddx49   Xenopus tropicalis Western clawed frog  Xtro\ddx49   Gallus gallus Domestic chicken  Ggal\DDX49   Mus musculus House mouse  Mmus\Ddx49   Rattus norvegicus Norway rat  Rnor\Ddx49   Homo sapiens Human  Hsap\DDX49
Human Orthologs (1)
Gene OMIM HGNC Hsap\DDX49
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10627 Drosophila sechellia Dsec\GM21090 Drosophila erecta Dere\GG23409 Drosophila yakuba Dyak\GE19250 Drosophila ananassae Dana\GF12227 Drosophila pseudoobscura pseudoobscura Dpse\GA11795 Drosophila persimilis Dper\GL17616 Drosophila willistoni Dwil\GK23245 Drosophila virilis Dvir\GJ21163 Drosophila mojavensis Dmoj\GI20069 Drosophila grimshawi Dgri\GH21617
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Kyoto	105428 y* w*; P{GawB}Dbp45ANP7328 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v104183 P{KK101176}VIE-260B v17306 w1118; P{GD7822}v17306
Genomic Clones ( 2 )
	BACR08E17 BACR10M14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 71 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47509
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM28554 LD01636 LD01637 LD13708 LD19854 LD41052 SD11850 SD19218
Other clones	21313 76483 98051 146103 EC051105 EC051617 EC054109 EC056170 EK139417 EK206841 EK224158 EK248846 RP004044922 RP004091410 RP004097291 RP004116690 RP004126434 RP004126647 RP004143713 RP004157756 RP004159618 RP004195172 RP004195462 RP004197614 RP004199532 RP004200213 RP004202033 RP004205147 RP004205505 RP004209685 RP004210949 RP004217052 RP004220747 RP004222102 RP004225967 RP004228774 RP004231447 RP004234030 RP004241129 RP004260765 RP004264501 RP004269874 RP004374043 RP004383734 RP004383879 RP004394411 RP004413857 RP004417476 RP004438095 RP004444128 RP004444752 RP004462742 RP004466071 RP004472690 RP004472981 RP004492698 RP004496714 RP004503868 RP004507119 RP004546980 RP004547377 RP004548465
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010220 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35917
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes. (Kliman and Eyre-Walker, 1998) Isolation and characterisation of Dbp45A. Dbp45A is most abundantly expressed in 6-12 hour embryos and adults. (Lavoie et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057579 RefSeq (Proteins) • NP_476927 DDBJ / EMBL / GenBank • AAA16339 AAF58994 AAL13957 AC005458 AC006074 AI515613 AW943909 AY058728 CAA80804 L13612 Z23266 Entrez Gene - A searchable database of RefSeq genes. • 35917 UniProtKB/Swiss-Prot • Q07886
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA helicase, ATP-dependent, DEAD-box, conserved site (IPR000629) Helicase, C-terminal (IPR001650) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) RNA helicase, DEAD-box type, Q motif (IPR014014) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD47509
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0010220 DRSC - Results from RNAi screens. • FBgn0010220 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010220 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12759-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010220 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35917 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35917 modMine - Data generated by the modENCODE project. • FBgn0010220
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG12759 (Keleman et al., 2009.8.5, McIntyre et al., 2006) Dbp45 (Eisen et al., 1998) DBP45A (Boudet et al., 2001) dbp45A (Lavoie, 1993) Dbp45A (McIntyre et al., 2006, Ashton-Beaucage et al., 2010, Japanese National Institute of Genetics, 2012.5.21) DmRH18 (Boudet et al., 2001)
Name Synonym	ATP-dependent RNA helicase (Lasko, 1993.7.9) DEAD box protein 45A
Secondary FlyBase IDs
References ( 28 )
Generate a list of	All references relating to this gene ( 28 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 3 ) Personal communication to FlyBase  ( 6 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...