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Gene Dmel\Nmdmc

General Information
Symbol	Dmel\Nmdmc	Species	D. melanogaster
Name	NAD-dependent methylenetetrahydrofolate dehydrogenase	Annotation symbol	CG18466
Feature type	protein_coding_gene	FlyBase ID	FBgn0010222
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	DNMDMC
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85C3-85C3	Sequence location	3R:4,868,230..4,876,277 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene NAD-dependent methylenetetrahydrofolate dehydrogenase is referred to in FlyBase by the symbol Dmel\Nmdmc (CG18466, FBgn0010222). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; methenyltetrahydrofolate cyclohydrolase activity. Based on sequence similarity, it is predicted to be involved in the biological process: 10-formyltetrahydrofolate metabolic process; carbohydrate metabolic process. 17 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: NAD(P)-binding domain; Tetrahydrofolate dehydrogenase/cyclohydrolase; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain; Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain; Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval fat body, virgin spermatheca, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval midgut, larval/adult hindgut, adult heart, adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640775_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Nmdmc. Gene sequence location is 3R:4868230..4876277.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms cytosol one-carbon metabolic process formate-tetrahydrofolate ligase activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85C3-85C3   Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473
Experimentally Determined Cytological Location
Cytogenetic map Notes References 85C-85C   (determined by in situ hybridisation)   (Price and Laughon, 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nmdmc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nmdmc-RA FBtr0081997  NM_057582   1156   303 Nmdmc-RB FBtr0081996  NM_057581   1257   309 Nmdmc-RC FBtr0336761  NM_001275469   1233   303
Additional Transcript Data & Comments
Reported size (kB)	1.25 (northern blot) (Price and Laughon, 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nmdmc-PA   FBpp0081477   32.8   303   7.27     NP_476930   AAF54332 Nmdmc-PB   FBpp0081476   33.6   309   7.60     NP_476929   AAN13408 Nmdmc-PC   FBpp0307737   32.8   303   7.27     NP_001262398   AGB95780
Additional Polypeptide Data & Comments
Reported size (kDa)	357 (aa); 39 (kD predicted) (Price and Laughon, 1993) 309, 303 (aa) (Hedengren et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Tetrahydrofolate dehydrogenase/cyclohydrolase (IPR000672) NAD(P)-binding domain (IPR016040) Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain (IPR020630) Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (IPR020631) Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved site (IPR020867) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME18466
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF54332   AE014297 AAN13408   AC009258   AF186073 AAF07929   AF186073 AAF07930   AE014297 AGB95780   AI062026   AI238333   AI513084   AY047498 AAK77230   BF501907   BI624429   BI634790   CA806254   CK131581   CL610201   CO179579   CO180711   CO180750   CO263178   CO263543   CO306956   CO321744   CZ467634   CZ473410   CZ478878   CZ482842   EC082223   EC082224   EC203386   EL881292   ES690454   ES691423   ES696576   ES696579   ES696582   GH941760   GH945327   GH948519   GH952636   GH954661   GH956470   GH962723   GH962873   GH964090   GH965210   GH965484   GH968310   GH968767   GH971531   GH974111   GH976114   GH984463   GH984915   L07958 AAB41352
UniProtKB/Swiss-Prot	Q04448
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) rectum primordium   (Fisher et al., 2012) Comment:reported as rectum specific anlage yolk nucleus   (Fisher et al., 2012) embryonic stage 13 -- 16   amnioserosa   (Fisher et al., 2012) embryonic rectum   (Fisher et al., 2012) yolk nucleus   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Price and Laughon, 1993)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
	Mitochondrial localization is predicted based on the presence of a mitochondrial targeting sequence, and on amino acid identity with mitochondrial proteins from other species. (Price and Laughon, 1993)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval fat body, virgin spermatheca, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval midgut, larval/adult hindgut, adult heart, adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body    1069 Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass    614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca    635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body  (1069) Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass  (614.525) Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca  (635.8) Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body  1069 Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass    614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca    635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body    1069 Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass    614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca    635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body    1069 Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass    614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca    635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body  (1069) Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass  614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca  635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body  1069 Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass    614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca    635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    187.625 Larval Midgut    143.7 Larval Hindgut    415.5 Larval Malpighian Tubules    63.9 Larval Fat Body    1069 Larval Salivary Gland    117.2 Larval Trachea    210.5 Larval Carcass    614.525 Adult Head    160.5 Adult Eye    197.8 Adult Brain    79.7 Adult Thoracic-Abdominal Ganglion    96.1 Adult Crop    172.5 Adult Midgut    31.8 Adult Hindgut    223.5 Adult Malpighian Tubules    51.6 Adult Fat Body    317.7 Adult Salivary Gland    78 Adult Heart    219.75 Adult VirginFemale Spermatheca    635.8 Adult InseminatedFemale Spermatheca    287.4 Adult Ovary    134.1 Adult Testis    86.9 Adult Male Accessory Gland    137.4 Adult Carcass    116.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    160.5   NA  Eye    197.8   NA  Brain    79.7   187.625  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    96.1   NA  Crop    172.5   143.7  Midgut    31.8   415.5  Hindgut    223.5   63.9  Malpighian Tubules    no informative data   1069  Fat Body    317.7   117.2  Salivary Gland    78   NA  Heart    219.75   210.5  Trachea    NA   NA  VirginFemale Spermatheca    635.8   NA  InseminatedFemale Spermatheca    287.4   NA  Ovary    134.1   NA  Testis    86.9   NA  Male Accessory Gland    137.4   614.525  Carcass    116.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female    52 head, mated 1-day female    22 head, mated 4-day female    68 head, mated 20-day female    36 head, mated 1-day male    30 head, mated 4-day male    55 head, mated 20-day male    39 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae    167 fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult    45 carcass, 4-day adult    51 carcass, 20-day adult    65 ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female  (52) head, mated 1-day female    22 head, mated 4-day female  (68) head, mated 20-day female  (36) head, mated 1-day male  (30) head, mated 4-day male  (55) head, mated 20-day male  (39) salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae  (167) fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult  (45) carcass, 4-day adult  (51) carcass, 20-day adult  (65) ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female    52 head, mated 1-day female    22 head, mated 4-day female    68 head, mated 20-day female    36 head, mated 1-day male    30 head, mated 4-day male    55 head, mated 20-day male    39 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae  (167) fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult    45 carcass, 4-day adult    51 carcass, 20-day adult    65 ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female    52 head, mated 1-day female    22 head, mated 4-day female    68 head, mated 20-day female    36 head, mated 1-day male    30 head, mated 4-day male    55 head, mated 20-day male    39 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae    167 fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult    45 carcass, 4-day adult    51 carcass, 20-day adult    65 ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female    52 head, mated 1-day female    22 head, mated 4-day female    68 head, mated 20-day female    36 head, mated 1-day male    30 head, mated 4-day male    55 head, mated 20-day male    39 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae    167 fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult    45 carcass, 4-day adult    51 carcass, 20-day adult    65 ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female  (52) head, mated 1-day female    22 head, mated 4-day female  (68) head, mated 20-day female  (36) head, mated 1-day male  30 head, mated 4-day male  (55) head, mated 20-day male  (39) salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae  (167) fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult  (45) carcass, 4-day adult  (51) carcass, 20-day adult  (65) ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female    52 head, mated 1-day female    22 head, mated 4-day female    68 head, mated 20-day female    36 head, mated 1-day male    30 head, mated 4-day male    55 head, mated 20-day male    39 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae  (167) fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult    45 carcass, 4-day adult    51 carcass, 20-day adult    65 ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    5 central nervous system, pupae P8    3 head, virgin 1-day female    23 head, virgin 4-day female    16 head, virgin 20-day female    52 head, mated 1-day female    22 head, mated 4-day female    68 head, mated 20-day female    36 head, mated 1-day male    30 head, mated 4-day male    55 head, mated 20-day male    39 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    9 digestive system, 4-day adult    7 digestive system, 20-day adult    8 fat body, larvae L3 wandering    12 fat body, white prepupae    167 fat body, pupae P8    21 carcass, larvae L3 wandering    25 carcass, 1-day adult    45 carcass, 4-day adult    51 carcass, 20-day adult    65 ovary, virgin 4-day female    5 ovary, mated 4-day female    4 testis, mated 4-day male    11 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr    29 embryo 08-10hr    26 embryo 10-12hr    46 embryo 12-14hr    30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr    36 embryo 22-24hr    27 larva L1    44 larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    93 white prepupae new    99 white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day    29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr  (29) embryo 08-10hr  26 embryo 10-12hr  (46) embryo 12-14hr  (30) embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr  (36) embryo 22-24hr  (27) larva L1  (44) larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (93) white prepupae new  (99) white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP  (47) pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day  (29) adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr    29 embryo 08-10hr    26 embryo 10-12hr    46 embryo 12-14hr    30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr    36 embryo 22-24hr    27 larva L1    44 larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    93 white prepupae new    99 white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day    29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr    29 embryo 08-10hr    26 embryo 10-12hr    46 embryo 12-14hr    30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr    36 embryo 22-24hr    27 larva L1    44 larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    93 white prepupae new    99 white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day    29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr    29 embryo 08-10hr    26 embryo 10-12hr    46 embryo 12-14hr    30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr    36 embryo 22-24hr    27 larva L1    44 larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    93 white prepupae new    99 white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day    29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr  29 embryo 08-10hr  26 embryo 10-12hr  (46) embryo 12-14hr  30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr  (36) embryo 22-24hr  27 larva L1  (44) larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (93) white prepupae new  (99) white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP  (47) pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day  29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr    29 embryo 08-10hr    26 embryo 10-12hr    46 embryo 12-14hr    30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr    36 embryo 22-24hr    27 larva L1    44 larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    93 white prepupae new    99 white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day    29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5 embryo 02-04hr    6 embryo 04-06hr    12 embryo 06-08hr    29 embryo 08-10hr    26 embryo 10-12hr    46 embryo 12-14hr    30 embryo 14-16hr    20 embryo 16-18hr    11 embryo 18-20hr    20 embryo 20-22hr    36 embryo 22-24hr    27 larva L1    44 larva L2    10 larva L3 12hr old    14 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    93 white prepupae new    99 white prepupae 12hr    21 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    13 pupae 4d postWPP    16 adult male 01day    13 adult male 05day    14 adult male 30day    20 adult female 01day    29 adult female 05day    20 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    57 Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2    30 embryonic Kc167    22 embryonic S1    32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2    40 wing disc ML-DmD8    11 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    49 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    466 tumorous blood cells mbn2    20 ovary fGS/OSS    30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (57) Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2  (30) embryonic Kc167    22 embryonic S1  (32) embryonic S3    13 leg disc CME L1    23 wing disc CME-W2  (40) wing disc ML-DmD8    11 wing disc ML-DmD9  (29) wing disc ML-DmD16-c3    17 wing disc ML-DmD21  (49) wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  (32) eye-antennal disc ML-DmD11  (31) antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1  (47) CNS ML-DmBG2-c2  (466) tumorous blood cells mbn2    20 ovary fGS/OSS  (30) ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    57 Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2    30 embryonic Kc167    22 embryonic S1    32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2    40 wing disc ML-DmD8    11 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    49 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2  (466) tumorous blood cells mbn2    20 ovary fGS/OSS    30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    57 Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2    30 embryonic Kc167    22 embryonic S1    32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2    40 wing disc ML-DmD8    11 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    49 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    466 tumorous blood cells mbn2    20 ovary fGS/OSS    30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    57 Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2    30 embryonic Kc167    22 embryonic S1    32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2    40 wing disc ML-DmD8    11 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    49 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    466 tumorous blood cells mbn2    20 ovary fGS/OSS    30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (57) Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2  30 embryonic Kc167    22 embryonic S1  32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2  (40) wing disc ML-DmD8    11 wing disc ML-DmD9  29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21  (49) wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  32 eye-antennal disc ML-DmD11  31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1  (47) CNS ML-DmBG2-c2  (466) tumorous blood cells mbn2    20 ovary fGS/OSS  30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    57 Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2    30 embryonic Kc167    22 embryonic S1    32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2    40 wing disc ML-DmD8    11 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    49 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2  (466) tumorous blood cells mbn2    20 ovary fGS/OSS    30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    57 Schneider line 2 Sg4    24 embryonic 1182-4H    18 embryonic GM2    30 embryonic Kc167    22 embryonic S1    32 embryonic S3    13 leg disc CME L1    23 wing disc CME-W2    40 wing disc ML-DmD8    11 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    49 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    31 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    466 tumorous blood cells mbn2    20 ovary fGS/OSS    30 ovary OSC    22 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3  (34) Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (31) Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (48) Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3  (34) Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (48) Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    17 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    31 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_25 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH01066 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010222 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18466-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Nmdmc Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable NmdmcCB02647 NmdmcEY08061 viable, with Scer\GAL4pnr-MD237 NmdmcGD1256 Sterility Allele fertile NmdmcEY08061 Phenotype manifest in Allele
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of Nmdmc Class Mutagen Stocks Known lesion Nmdmcc02037 piggyBac transposase 1 -- NmdmcCB-5723-3 1 -- Nmdmce01052 piggyBac transposase 1 -- NmdmcEY08061 P-element activity 1 Yes Nmdmcf00375 piggyBac transposase 1 -- NmdmcNP2138 1 -- NmdmcCB02647 P-element activity 0 Yes Nmdmcd03528 P-element activity 0 -- NmdmcE14 Delta2-3 0 Yes NmdmcE20 Delta2-3 0 Yes NmdmcE21 Delta2-3 0 Yes NmdmcE26 Delta2-3 0 Yes NmdmcE38 Delta2-3 0 Yes NmdmcL3141a P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nmdmc Class Mutagen Stocks Known lesion NmdmcGD1256 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes NmdmcKK102478 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes NmdmcNIG.18466R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6258 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1256} NA P{KK102478} NA P{NIG.18466R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}NmdmcEY08061 NA P{XP}Nmdmcd03528 NA miscellaneous insertions P{PTT-GB}NmdmcCB02647 NA P{RS3}NmdmcCB-5723-3 NA PBac{PB}Nmdmcc02037 NA PBac{RB}Nmdmce01052 NA PBac{WH}Nmdmcf00375 NA insertion of enhancer trap binary system P{GawB}NmdmcNP2138 No insertion of enhancer trap P{lacW}RelL3141a No
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 12 terms )
Molecular Function
CV term Evidence References
NOT formate-tetrahydrofolate ligase activity inferred from biological aspect of ancestor with PANTHER:PTN000002327 (assigned by RefGenome) (Gaudet et al., 2010) magnesium ion binding non-traceable author statement (Swiss-Prot Project Members, 1993.10.1) methenyltetrahydrofolate cyclohydrolase activity inferred from biological aspect of ancestor with PANTHER:PTN000002250 (assigned by RefGenome) (Gaudet et al., 2010) inferred from sequence or structural similarity (Price and Laughon, 1993) inferred from sequence or structural similarity with UniProtKB:P13995 (Sardiello et al., 2003.6.11) inferred from sequence or structural similarity with UniProtKB:P18155 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.10.1) methylenetetrahydrofolate dehydrogenase (NAD+) activity inferred from biological aspect of ancestor with PANTHER:PTN000002327 (assigned by RefGenome) (Gaudet et al., 2010) non-traceable author statement (Swiss-Prot Project Members, 1993.10.1) methylenetetrahydrofolate dehydrogenase (NADP+) activity inferred from biological aspect of ancestor with PANTHER:PTN000002250 (assigned by RefGenome) (Gaudet et al., 2010) inferred from sequence or structural similarity with UniProtKB:P18155 (FlyBase, 1992-) non-traceable author statement (Price, 1993)
Biological Process
CV term Evidence References
10-formyltetrahydrofolate metabolic process inferred from sequence or structural similarity with UniProtKB:P13995 (Sardiello et al., 2003.6.11) carbohydrate metabolic process inferred from sequence or structural similarity with UniProtKB:P13995 (Sardiello et al., 2003.6.11) folic acid-containing compound biosynthetic process inferred from electronic annotation with InterPro:IPR000672, InterPro:IPR020630, InterPro:IPR020631, InterPro:IPR020867 (FlyBase Curators et al., 2004-) one-carbon metabolic process inferred from biological aspect of ancestor with PANTHER:PTN000002250 (assigned by RefGenome) (Gaudet et al., 2010) oxidation-reduction process inferred from electronic annotation with InterPro:IPR000672, InterPro:IPR020630, InterPro:IPR020631, InterPro:IPR020867 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
NOT cytosol inferred from biological aspect of ancestor with PANTHER:PTN000002327 (assigned by RefGenome) (Gaudet et al., 2010) mitochondrion inferred from biological aspect of ancestor with PANTHER:PTN000002327 (assigned by RefGenome) (Gaudet et al., 2010) inferred from sequence or structural similarity (Price and Laughon, 1993) inferred from sequence or structural similarity with UniProtKB:P13995 (Sardiello et al., 2003.6.11) non-traceable author statement (Swiss-Prot Project Members, 1993.10.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Nmdmc - CG13659 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nmdmc - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nmdmc - Smn experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Nmdmc - aur anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nmdmc - chrb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nmdmc allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010222 DroID - A comprehensive database of gene and protein interactions. • FBgn0010222 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47895
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6CVGT0 Dipteran inclusive ortholog search EOG6N5W5P Insect inclusive ortholog search EOG6BG7RQ Arthropod inclusive ortholog search EOG6Q83D4 Metazoa inclusive ortholog search EOG67HJHZ
Orthologs in Drosophila Species (EOG6CVGT0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Nmdmc     Drosophila simulans   Dsim\GD20823 Y   Drosophila sechellia   Dsec\GM26287 Y   Drosophila erecta   Dere\GG17397 Y   Drosophila yakuba   Dyak\GE24801 Y   Drosophila ananassae   Dana\GF18431 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14943 Y   Drosophila persimilis   Dper\GL12491 Y   Drosophila willistoni   Dwil\GK14062 Y   Drosophila virilis   Dvir\GJ23482 Y   Drosophila mojavensis   Dmoj\GI10194 Y   Drosophila grimshawi   Dgri\GH18238 Y
Orthologs in non-Drosophila Dipterans (EOG6N5W5P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010751   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014871   Anopheles gambiae Malaria mosquito  Agam\AGAP004677   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004709   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004710
Orthologs in non-Dipteran Insects (EOG6BG7RQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Nmdmc   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002428   Acromyrmex echinatior Panamanian leafcutter ant  Aech\folD   Camponotus floridanus Florida carpenter ant  Cflo\folD   Harpegnathos saltator Jerdons jumping ant  Hsal\folD   Linepithema humile Argentine ant  Lhum\LH17540   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24642   Solenopsis invicta Red fire ant  Sinv\SINV15001   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005385   Bombyx mori Silkmoth  Bmor\BGIBMGA002355   Pediculus humanus Human body louse  Phum\PHUM125130   Tribolium castaneum Red flour beetle  Tcas\TC005370
Orthologs in non-Insect Arthropods (EOG6Q83D4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW022040
Orthologs in non-Arthropod Metazoa (EOG67HJHZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010209gn   Danio rerio Zebrafish  Drer\mthfd2   Danio rerio Zebrafish  Drer\mthfd2l   Danio rerio Zebrafish  Drer\mthfd2   Xenopus tropicalis Western clawed frog  Xtro\mthfd2   Xenopus tropicalis Western clawed frog  Xtro\mthfd2l   Gallus gallus Domestic chicken  Ggal\MTHFD2L   Gallus gallus Domestic chicken  Ggal\ENSGALG00000013480   Mus musculus House mouse  Mmus\Mthfd2   Mus musculus House mouse  Mmus\Mthfd2l   Rattus norvegicus Norway rat  Rnor\Mthfd2l   Rattus norvegicus Norway rat  Rnor\Mthfd2   Homo sapiens Human  Hsap\ENSG00000163738   Homo sapiens Human  Hsap\MTHFD2
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000163738     Hsap\MTHFD2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20823 Drosophila sechellia Dsec\GM26287 Drosophila erecta Dere\GG17397 Drosophila yakuba Dyak\GE24801 Drosophila ananassae Dana\GF18431 Drosophila pseudoobscura pseudoobscura Dpse\GA14943 Drosophila persimilis Dper\GL12491 Drosophila willistoni Dwil\GK14062 Drosophila virilis Dvir\GJ23482 Drosophila mojavensis Dmoj\GI10194 Drosophila grimshawi Dgri\GH18238
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	20016 y1 w67c23; P{EPgy2}NmdmcEY08061 RelEY08061
Harvard	- PBac{PB}Nmdmcc02037 - PBac{RB}Nmdmce01052 - PBac{WH}Nmdmcf00375
Kyoto	123779 w1118; P{RS3}NmdmcCB-5723-3 104103 w*; P{GawB}NmdmcNP2138 / TM3, Sb1 Ser1
VDRC	v5705 w1118; P{GD1256}v5705 v5706 w1118; P{GD1256}v5706
Genomic Clones ( 1 )
	BACR09F04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 35 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01066
Other clones	DPiM_GH01066
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT17486 GH14359 LP11492 RH39026 RH63983
Other clones	EC03366 EC03666 EK059902 EK087708 EK088757 EK186307 EK252851 ESG012c.E21_C02 IP11083 RC0-DA0001-120700-021-B01 RP004078188 RP004110792 RP004141729 RP004195458 RP004198505 RP004232135 RP004263133 RP004327081 RP004370149 RP004409960 RP004450778 RP004456857 RP004491071 RP004495341 RP004506699 RP004513768 RP004520940 RP004522188
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010222 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47895
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Nmdmc CG18466 (Misra, 2000.4.14)
Additional comments	pug is a different gene from Nmdmc; pug (at 86C) appears to encode the cytoplasmic form of methylenetetrahydrofolate dehydrogenase, Nmdmc (at 85C) appears to encode the mitochondrial form of methylenetetrahydrofolate dehydrogenase. (Rong and Golic, 1998)
Other Comments
	Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. (McDonald and Rosbash, 2001) Nmdmc enzyme activity in the S3 cell line has been analysed. (Tremblay et al., 1995) Identification: Part of molecular analysis of the neur genomic region. (Price and Laughon, 1993) Nmdmc has been cloned and sequenced. (Price and Laughon, 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057581 NM_057582 NM_001275469 RefSeq (Proteins) • NP_476929 NP_476930 NP_001262398 DDBJ / EMBL / GenBank • AAB41352 AAF07929 AAF07930 AAF54332 AAK77230 AAN13408 AC009258 AF186073 AGB95780 AI062026 AI513084 AY047498 CL610201 CZ467634 CZ473410 CZ478878 CZ482842 L07958 Entrez Gene - A searchable database of RefSeq genes. • 47895 UniProtKB/Swiss-Prot • Q04448
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Tetrahydrofolate dehydrogenase/cyclohydrolase (IPR000672) NAD(P)-binding domain (IPR016040) Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain (IPR020630) Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (IPR020631) Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved site (IPR020867) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME18466 BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH01066
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010222 DroID - A comprehensive database of gene and protein interactions. • FBgn0010222 DRSC - Results from RNAi screens. • FBgn0010222 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010222 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18466-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010222 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47895 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47895 modMine - Data generated by the modENCODE project. • FBgn0010222
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	151767_at (Kristensen et al., 2005) CG18466 (Whitfield, 2000.5.16, Wittkopp et al., 2008, Curtis et al., 2007, Keleman et al., 2009.8.5) DNMDMC (Price, 1993, Price et al., 1993, Price and Laughon, 1993) dNMDMC (Tremblay et al., 1995) Dnmdmc   Nmdmc (Gruenewald et al., 2009, Buszczak et al., 2007, Weiler, 2007, Wu et al., 2007, Kasuya et al., 2009, McManus et al., 2010, Wittkopp et al., 2008, Kim et al., 2005, Curtis et al., 2007, Mizrak et al., 2012, Japanese National Institute of Genetics, 2012.5.21) nmdmc (Beckstead et al., 2007, Rehwinkel et al., 2005) unnamed (Rong and Golic, 1998)
Name Synonym	NAD-dependent methylenetetrahydrofolate dehydrogenase   NAD dependent methylenetetrahydrofolate dehydrogenase (Kasuya et al., 2009)
Secondary FlyBase IDs
	FBgn0037658
References ( 49 )
Generate a list of	All references relating to this gene ( 49 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 1 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 2 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Mizrak et al., 2012, Curr. Biol. 22(20): 1871--1880 Electrical activity can impose time of day on the circadian transcriptome of pacemaker neurons. [FBrf0219786] Show all research papers ...