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Gene Dmel\Myo61F

General Information
Symbol	Dmel\Myo61F	Species	D. melanogaster
Name	Myosin 61F	Annotation symbol	CG9155
Feature type	protein_coding_gene	FlyBase ID	FBgn0010246
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	Myo1B, DroMIB
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	61F6-61F6	Sequence location	3L:1,318,480..1,329,617 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Myosin 61F is referred to in FlyBase by the symbol Dmel\Myo61F (CG9155, FBgn0010246). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity, coupled. There is experimental evidence that it is involved in the biological process: determination of left/right symmetry; imaginal disc-derived male genitalia morphogenesis; defense response to bacterium; microvillus organization; mesoderm development. 21 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/larval midgut; embryonic hindgut; enterocyte; embryonic/larval midgut primordium; microvillus; male genitalia; brush border. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: IQ motif, EF-hand binding site; Myosin head, motor domain; Myosin tail 2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval/adult hindgut. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1638278_s_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Myo61F. Gene sequence location is 3L:1318480..1329617.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 61F6-61F6   Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
Experimentally Determined Cytological Location
Cytogenetic map Notes References 61F-61F     (Kravit, 1993) 61F-61F     (Caggese, 1997.7.29) 61F-61F   (determined by in situ hybridisation)   (Morgan et al., 1994) 61F-61F   (determined by in situ hybridisation)   (Cheney et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Myo61F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Low-frequency RNA-seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Myo61F-RA FBtr0072675  NM_167870   3446   1035 Myo61F-RB FBtr0072673  NM_167871   3454   1026 Myo61F-RC FBtr0072672  NM_167872   3592   1026 Myo61F-RD FBtr0072674  NM_057586   3369   1052
Additional Transcript Data & Comments
Reported size (kB)	3.5 (northern blot) (Morgan et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Myo61F-PA   FBpp0072568   119.0   1035   9.45     NP_728594   AAF47477 Myo61F-PB   FBpp0072566   118.0   1026   9.41     NP_728595   AAN11472 Myo61F-PC   FBpp0072565   118.0   1026   9.41     NP_728596   AAN11473 Myo61F-PD   FBpp0072567   121.1   1052   9.35     NP_476934   AAF47478
Additional Polypeptide Data & Comments
Reported size (kDa)	1026 (aa); 118 (kD predicted) (Morgan et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • IQ motif, EF-hand binding site (IPR000048) Myosin head, motor domain (IPR001609) Myosin tail 2 (IPR010926) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140980   AE014296 AAF47477   AE014296 AAF47478   AE014296 AAN11472   AE014296 AAN11473   AC004489   AC005847   AI063969   AI106829   AI135782   AI135790   AI257405   AI258599   AI294396   AI295145   AI387677   AI404096   AI405567   AI405811   AJ000879 CAA04367   AQ073914   AW940162   AY069044 AAL39189   BG639994   BI166676   BI167423   BI173198   BI228037   BI229515   BI230752   BI231167   BI232554   BI235055   BI238304   BI242571   BI243066   BI357213   BI366619   BI372740   BI372815   BI372918   BI374442   BI581336   BI581346   BI581845   BI592919   BI611753   BI611872   BI628837   BP555192   BT133300   CK657226   CK657528   CK658715   CK660131   CK661662   CK661675   CK662191   CO153080   CO304171   CO312325   CO312874   CO313907   CO331805   CO336463   CO337328   CO340955   EC090582   EC202223   EC202545   EC224670   EC248418   EC248601   EC258224   EC260825   GH771564   GH875871   L13070   U07596 AAA19591
UniProtKB/Swiss-Prot	Q23979
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) gonad   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Morgan et al., 1994)
Additional Descriptive Data
	Myo61F transcripts are first observed in 8-12hr embryos, increase until the third instar larval stage and are present at a lower level in adults. (Morgan et al., 1994)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval digestive system   (Morgan et al., 1995) embryonic stage | late   mid embryonic/larval midgut   (Morgan et al., 1995) posterior embryonic/larval midgut   (Morgan et al., 1995) gastric caecum   (Morgan et al., 1995) anterior embryonic/larval midgut   (Morgan et al., 1995) embryonic/larval hindgut   (Morgan et al., 1995) embryonic stage -- adult stage     (Morgan et al., 1995) adult stage   alimentary canal   (Morgan et al., 1995) oogenesis   centripetally migrating follicle cell   (Morgan et al., 1995) follicle cell   (Morgan et al., 1995) egg chamber   (Morgan et al., 1995)
Additional Descriptive Data
	At embryonic stage 16, Myo61F protein is concentrated at the basolateral domain of immature enterocytes and is diffusely located in the cytoplasm. At embryonic stage 17, intense apical staining is observed in most regions of the gut. In larvae and adults, Myo61F protein is found in the brush border predominantly in the apical microvillar domain. In egg chambers, it is found in the basolateral domain of follicle cells until stage 8 after which it is more apically positioned. In stage 10, the localization of Myo61F protein to the api al domain of columnar follicle cells near the oocyte is thought to correspond to the microvilli of the brush border which exists there. (Morgan et al., 1995)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
brush border inferred from direct assay (Hegan et al., 2007) cell cortex inferred from direct assay (Petzoldt et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval/adult hindgut. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut    2891.9 Larval Hindgut    920.5 Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut    3676.2 Adult Hindgut    1214.7 Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut  (2891.9) Larval Hindgut  (920.5) Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut  (3676.2) Adult Hindgut  (1214.7) Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut  (2891.9) Larval Hindgut    920.5 Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut  (3676.2) Adult Hindgut  (1214.7) Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut    2891.9 Larval Hindgut    920.5 Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut    3676.2 Adult Hindgut    1214.7 Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut    2891.9 Larval Hindgut    920.5 Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut    3676.2 Adult Hindgut    1214.7 Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut  (2891.9) Larval Hindgut  (920.5) Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut  (3676.2) Adult Hindgut  (1214.7) Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut  (2891.9) Larval Hindgut    920.5 Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut  (3676.2) Adult Hindgut  1214.7 Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    27.775 Larval Midgut    2891.9 Larval Hindgut    920.5 Larval Malpighian Tubules    270.6 Larval Fat Body    50.9 Larval Salivary Gland    446.6 Larval Trachea    323.3 Larval Carcass    203.725 Adult Head    57.2 Adult Eye    20.75 Adult Brain    4.1 Adult Thoracic-Abdominal Ganglion    6.3 Adult Crop    4.3 Adult Midgut    3676.2 Adult Hindgut    1214.7 Adult Malpighian Tubules    276.8 Adult Fat Body    167.6 Adult Salivary Gland    32.2 Adult Heart    108.1 Adult VirginFemale Spermatheca    142.2 Adult InseminatedFemale Spermatheca    153 Adult Ovary    45.6 Adult Testis    53.4 Adult Male Accessory Gland    195.2 Adult Carcass    72.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    57.2   NA  Eye    20.75   NA  Brain    4.1   27.775  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.3   NA  Crop    4.3   2891.9  Midgut    3676.2   920.5  Hindgut    1214.7   270.6  Malpighian Tubules    276.8   50.9  Fat Body    167.6   446.6  Salivary Gland    no informative data   NA  Heart    108.1   323.3  Trachea    NA   NA  VirginFemale Spermatheca    142.2   NA  InseminatedFemale Spermatheca    153   NA  Ovary    45.6   NA  Testis    53.4   NA  Male Accessory Gland    195.2   203.725  Carcass    72.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    177 digestive system, 4-day adult    157 digestive system, 20-day adult    181 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    40 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae  (27) digestive system, larvae L3 wandering  (63) digestive system, 1-day adult  (177) digestive system, 4-day adult  (157) digestive system, 20-day adult  (181) fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering  (40) carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    63 digestive system, 1-day adult  (177) digestive system, 4-day adult  (157) digestive system, 20-day adult  (181) fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    40 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    177 digestive system, 4-day adult    157 digestive system, 20-day adult    181 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    40 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    177 digestive system, 4-day adult    157 digestive system, 20-day adult    181 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    40 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae  27 digestive system, larvae L3 wandering  (63) digestive system, 1-day adult  (177) digestive system, 4-day adult  (157) digestive system, 20-day adult  (181) fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering  (40) carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    63 digestive system, 1-day adult  (177) digestive system, 4-day adult  (157) digestive system, 20-day adult  (181) fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    40 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    5 central nervous system, pupae P8    1 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    9 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    177 digestive system, 4-day adult    157 digestive system, 20-day adult    181 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    3 carcass, larvae L3 wandering    40 carcass, 1-day adult    6 carcass, 4-day adult    6 carcass, 20-day adult    9 ovary, virgin 4-day female    6 ovary, mated 4-day female    4 testis, mated 4-day male    10 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr    38 embryo 18-20hr    81 embryo 20-22hr    140 embryo 22-24hr    137 larva L1    73 larva L2    111 larva L3 12hr old    63 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    45 white prepupae new    34 white prepupae 12hr    27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    26 adult male 01day    49 adult male 05day    57 adult male 30day    44 adult female 01day    57 adult female 05day    36 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr  (38) embryo 18-20hr  (81) embryo 20-22hr  (140) embryo 22-24hr  (137) larva L1  (73) larva L2  (111) larva L3 12hr old  (63) larva L3 puffstage 1-2  (53) larva L3 puffstage 3-6  (42) larva L3 puffstage 7-9  (45) white prepupae new  (34) white prepupae 12hr  (27) white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP  26 adult male 01day  (49) adult male 05day  (57) adult male 30day  (44) adult female 01day  (57) adult female 05day  (36) adult female 30day  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr    38 embryo 18-20hr    81 embryo 20-22hr  (140) embryo 22-24hr  (137) larva L1    73 larva L2  (111) larva L3 12hr old    63 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    45 white prepupae new    34 white prepupae 12hr    27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    26 adult male 01day    49 adult male 05day    57 adult male 30day    44 adult female 01day    57 adult female 05day    36 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr    38 embryo 18-20hr    81 embryo 20-22hr    140 embryo 22-24hr    137 larva L1    73 larva L2    111 larva L3 12hr old    63 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    45 white prepupae new    34 white prepupae 12hr    27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    26 adult male 01day    49 adult male 05day    57 adult male 30day    44 adult female 01day    57 adult female 05day    36 adult female 30day    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr    38 embryo 18-20hr    81 embryo 20-22hr    140 embryo 22-24hr    137 larva L1    73 larva L2    111 larva L3 12hr old    63 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    45 white prepupae new    34 white prepupae 12hr    27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    26 adult male 01day    49 adult male 05day    57 adult male 30day    44 adult female 01day    57 adult female 05day    36 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr  (38) embryo 18-20hr  (81) embryo 20-22hr  (140) embryo 22-24hr  (137) larva L1  (73) larva L2  (111) larva L3 12hr old  (63) larva L3 puffstage 1-2  (53) larva L3 puffstage 3-6  (42) larva L3 puffstage 7-9  (45) white prepupae new  (34) white prepupae 12hr  27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP  26 adult male 01day  (49) adult male 05day  (57) adult male 30day  (44) adult female 01day  (57) adult female 05day  (36) adult female 30day  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr    38 embryo 18-20hr    81 embryo 20-22hr  140 embryo 22-24hr  137 larva L1    73 larva L2  111 larva L3 12hr old    63 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    45 white prepupae new    34 white prepupae 12hr    27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    26 adult male 01day    49 adult male 05day    57 adult male 30day    44 adult female 01day    57 adult female 05day    36 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    2 embryo 08-10hr    3 embryo 10-12hr    11 embryo 12-14hr    12 embryo 14-16hr    21 embryo 16-18hr    38 embryo 18-20hr    81 embryo 20-22hr    140 embryo 22-24hr    137 larva L1    73 larva L2    111 larva L3 12hr old    63 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    45 white prepupae new    34 white prepupae 12hr    27 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    26 adult male 01day    49 adult male 05day    57 adult male 30day    44 adult female 01day    57 adult female 05day    36 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    102 Schneider line 2 Sg4    16 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    37 embryonic S1    67 embryonic S3    4 leg disc CME L1    25 wing disc CME-W2    46 wing disc ML-DmD8    13 wing disc ML-DmD9    40 wing disc ML-DmD16-c3    71 wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    97 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    72 ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (102) Schneider line 2 Sg4    16 embryonic 1182-4H  (41) embryonic GM2  (78) embryonic Kc167  (37) embryonic S1  (67) embryonic S3    4 leg disc CME L1    25 wing disc CME-W2  (46) wing disc ML-DmD8    13 wing disc ML-DmD9  (40) wing disc ML-DmD16-c3  (71) wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11  (97) antennal disc ML-DmD20-c5  (30) mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2  (36) tumorous blood cells mbn2  (72) ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  102 Schneider line 2 Sg4    16 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    37 embryonic S1    67 embryonic S3    4 leg disc CME L1    25 wing disc CME-W2    46 wing disc ML-DmD8    13 wing disc ML-DmD9    40 wing disc ML-DmD16-c3    71 wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    97 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    72 ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    102 Schneider line 2 Sg4    16 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    37 embryonic S1    67 embryonic S3    4 leg disc CME L1    25 wing disc CME-W2    46 wing disc ML-DmD8    13 wing disc ML-DmD9    40 wing disc ML-DmD16-c3    71 wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    97 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    72 ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    102 Schneider line 2 Sg4    16 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    37 embryonic S1    67 embryonic S3    4 leg disc CME L1    25 wing disc CME-W2    46 wing disc ML-DmD8    13 wing disc ML-DmD9    40 wing disc ML-DmD16-c3    71 wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    97 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    72 ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (102) Schneider line 2 Sg4    16 embryonic 1182-4H  (41) embryonic GM2  (78) embryonic Kc167  (37) embryonic S1  (67) embryonic S3    4 leg disc CME L1    25 wing disc CME-W2  (46) wing disc ML-DmD8    13 wing disc ML-DmD9  (40) wing disc ML-DmD16-c3  (71) wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11  (97) antennal disc ML-DmD20-c5  30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2  (36) tumorous blood cells mbn2  (72) ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  102 Schneider line 2 Sg4    16 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    37 embryonic S1    67 embryonic S3    4 leg disc CME L1    25 wing disc CME-W2    46 wing disc ML-DmD8    13 wing disc ML-DmD9    40 wing disc ML-DmD16-c3    71 wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    97 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    72 ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    102 Schneider line 2 Sg4    16 embryonic 1182-4H    41 embryonic GM2    78 embryonic Kc167    37 embryonic S1    67 embryonic S3    4 leg disc CME L1    25 wing disc CME-W2    46 wing disc ML-DmD8    13 wing disc ML-DmD9    40 wing disc ML-DmD16-c3    71 wing disc ML-DmD21    5 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    97 antennal disc ML-DmD20-c5    30 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    72 ovary fGS/OSS    25 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    26 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    48 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    41 Rotenone 8 μg 12 hrs, larvae L3    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult  (29) Cadmium 50 mM 6 hrs, larvae L3  (35) Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult  (37) Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3  (29) Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3  26 Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3  (48) Ethanol 10% 3 hrs, larvae L3  (38) Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult  (28) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3  (41) Rotenone 8 μg 12 hrs, larvae L3  (58) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    26 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    48 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    41 Rotenone 8 μg 12 hrs, larvae L3    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    26 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    48 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    41 Rotenone 8 μg 12 hrs, larvae L3    58 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    26 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    48 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    41 Rotenone 8 μg 12 hrs, larvae L3    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult  29 Cadmium 50 mM 6 hrs, larvae L3  (35) Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult  (37) Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3  29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3  26 Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3  (48) Ethanol 10% 3 hrs, larvae L3  (38) Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult  28 Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3  (41) Rotenone 8 μg 12 hrs, larvae L3  (58) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    26 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    48 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    41 Rotenone 8 μg 12 hrs, larvae L3    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    13 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    37 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    29 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    26 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    48 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    23 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    12 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    41 Rotenone 8 μg 12 hrs, larvae L3    58 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • GH04201 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010246 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9155-RC FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Myo61F Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Myo61FGD4045 Other Phenotypes Allele axis specification defective | embryonic stage, with Scer\GAL4byn-Gal4 Myo61FScer\UAS.T:Ivir\HA1 Myo61FΔIQ.Scer\UAS.T:Ivir\HA1 increased cell death | larval stage Myo61F920 Phenotype manifest in Allele brush border Myo61F920 embryonic/larval midgut Myo61F920 embryonic/larval midgut, with Scer\GAL4byn-Gal4 Myo61FScer\UAS.T:Ivir\HA1 Myo61FΔIQ.Scer\UAS.T:Ivir\HA1 embryonic/larval midgut primordium, with Scer\GAL4byn-Gal4 Myo61FScer\UAS.cHa embryonic/larval midgut primordium, with Scer\GAL4nos.PG Myo61FdsRNA.Scer\UAS embryonic hindgut, with Scer\GAL4byn-Gal4 Myo61FScer\UAS.cHa Myo61FScer\UAS.T:Ivir\HA1 Myo61FΔIQ.Scer\UAS.T:Ivir\HA1 enterocyte Myo61F920 male genitalia, with Scer\GAL4Abd-B-LDN Myo61FScer\UAS.cHa male genitalia, with Scer\GAL4Myo31DF-NP1548 Myo61FScer\UAS.cHa Myo61FScer\UAS.T:Disc\RFP male genitalia, with Scer\GAL4tsh-md621 Myo61FScer\UAS.cHa microvillus Myo61F920
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of Myo61F Class Mutagen Stocks Known lesion Myo61Fc03296 1 -- Myo61FGS9889 1 Yes Myo61FNP1320 1 -- Myo61F920 Delta2-3 0 Yes Myo61FEP3325a P-element activity 0 Yes Myo61FEP3325b 0 --
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Myo61F Class Mutagen Stocks Known lesion Myo61FGD4045 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Myo61FHMS02253 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Myo61F+t5.7 in vitro construct - genomic fragment 0 Yes Myo61FdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes Myo61FKK101033 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Myo61FmATPbs.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Myo61FScer\UAS.cHa in vitro construct - regulatory fusion 0 Yes Myo61FScer\UAS.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Myo61FScer\UAS.T:Ivir\HA1 0 Yes Myo61FTail.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Myo61Ftail.Scer\UAS.T:Ivir\HA1 0 Yes Myo61FΔActinbs.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes Myo61FΔIQ.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes Myo61FΔP-loop.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes Myo61FΔtail.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3L)920 (Hegan et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4045} NA P{KK101033} NA P{TRiP.HMS02253} NA P{UAS-Myo61F.dsRNA} NA P{UAS-Myo61F.GFP.Tail} NA P{UAS-Myo61F.H} NA P{UAS-Myo61F.HA} NA P{UAS-Myo61F.mATPbs.HA} NA P{UAS-Myo61F.RFP} NA P{UAS-Myo61F.tail.HA} NA P{UAS-Myo61F.ΔActinbs.HA} NA P{UAS-Myo61F.ΔIQ.HA} NA P{UAS-Myo61F.ΔP-loop.HA} NA P{UAS-Myo61F.Δtail.HA} NA characterization construct P{Myo61F+t5.7} NA P{UAS-Myo31DF::Myo61F.31DFIQtail} NA P{UAS-Myo31DF::Myo61F.61FIQtail} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Myo61FEP3325a NA P{EP}Myo61FEP3325b NA P{GSV6}Myo61FGS9889 NA insertion of enhancer trap binary system P{GawB}Myo61FNP1320 No miscellaneous insertions PBac{PB}Myo61Fc03296 NA
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
defense response to bacterium inferred from mutant phenotype (Hegan et al., 2007) determination of left/right symmetry inferred from mutant phenotype (Hozumi et al., 2006, Taniguchi et al., 2007, Petzoldt et al., 2012) imaginal disc-derived male genitalia morphogenesis inferred from mutant phenotype (Petzoldt et al., 2012) mesoderm development inferred from expression pattern (Furlong et al., 2001) microvillus organization inferred from mutant phenotype (Hegan et al., 2007)
Cellular Component
CV term Evidence References
brush border inferred from direct assay (Hegan et al., 2007) cell cortex inferred from direct assay (Petzoldt et al., 2012)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
actin binding non-traceable author statement (Kravit, 1993, Morgan, 1994.3.8, Swiss-Prot Project Members, 2000.10.1) ATPase activity, coupled inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) ATP binding inferred from electronic annotation with InterPro:IPR001609 (FlyBase Curators et al., 2004-) motor activity inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR010926 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) myosin complex inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR010926 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Myo61F - 7B2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - Arp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG10641 experimental knowledge based (Guruharsha et al., 2011) Myo61F - CG14823 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG1909 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG3303 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG3501 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG5037 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG5646 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - CG9135 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - Cortactin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - Orc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - PHGPx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - Pi3K59F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - Rh5 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Myo61F - Rpp30 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Myo61F - lark anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Myo61F allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0010246 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38153
Orthologs
OrthoDB Orthologs (66) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6XSM88 Dipteran inclusive ortholog search EOG66Q71K Insect inclusive ortholog search EOG6HQCD6 Arthropod inclusive ortholog search EOG6V15FQ Metazoa inclusive ortholog search EOG6W71NX
Orthologs in Drosophila Species (EOG6XSM88)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Myo61F     Drosophila simulans   Dsim\GD13610     Drosophila sechellia   Dsec\GM14400 Y   Drosophila erecta   Dere\GG14781 Y   Drosophila yakuba   Dyak\GE21145 Y   Drosophila ananassae   Dana\GF24454 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21581 Y   Drosophila persimilis   Dper\GL16178     Drosophila willistoni   Dwil\GK13346 Y   Drosophila virilis   Dvir\GJ12149 Y   Drosophila mojavensis   Dmoj\GI13054 Y   Drosophila grimshawi   Dgri\GH15215 Y
Orthologs in non-Drosophila Dipterans (EOG66Q71K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011905   Anopheles gambiae Malaria mosquito  Agam\AGAP011725   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015035
Orthologs in non-Dipteran Insects (EOG6HQCD6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Myo1B   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15277   Atta cephalotes Leafcutter ant  Acep\ACEP16607   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17826   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14267   Linepithema humile Argentine ant  Lhum\LH12269   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12628   Acyrthosiphon pisum Pea aphid  Apim\ACYPI081577   Acyrthosiphon pisum Pea aphid  Apim\ACYPI071352   Bombyx mori Silkmoth  Bmor\BGIBMGA009857   Tribolium castaneum Red flour beetle  Tcas\TC011553
Orthologs in non-Insect Arthropods (EOG6V15FQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\rbcL   Daphnia pulex Water flea  Dpux\matK   Daphnia pulex Water flea  Dpux\COII   Ixodes scapularis Deer tick  Isca\ISCW010768
Orthologs in non-Arthropod Metazoa (EOG6W71NX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\hum-5 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014397gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019273gn Y Danio rerio Zebrafish  Drer\si:dkey-202g15.10 Y Danio rerio Zebrafish  Drer\si:dkeyp-92c9.3 Y Danio rerio Zebrafish  Drer\myo1bl2 Y Danio rerio Zebrafish  Drer\myo1c Y Danio rerio Zebrafish  Drer\myo1ha Y Danio rerio Zebrafish  Drer\myo1c Y Danio rerio Zebrafish  Drer\ENSDARG00000036863 Y Xenopus tropicalis Western clawed frog  Xtro\myo1c Y Xenopus tropicalis Western clawed frog  Xtro\myo1a Y Xenopus tropicalis Western clawed frog  Xtro\myo1h Y Xenopus tropicalis Western clawed frog  Xtro\myo1d Y Gallus gallus Domestic chicken  Ggal\MYO1D Y Gallus gallus Domestic chicken  Ggal\MYO1G Y Gallus gallus Domestic chicken  Ggal\MYO1B Y Gallus gallus Domestic chicken  Ggal\MYO1C Y Gallus gallus Domestic chicken  Ggal\MYO1H Y Mus musculus House mouse  Mmus\Myo1g Y Mus musculus House mouse  Mmus\Myo1h Y Mus musculus House mouse  Mmus\Myo1d Y Mus musculus House mouse  Mmus\Myo1a Y Mus musculus House mouse  Mmus\Myo1c Y Mus musculus House mouse  Mmus\Myo1b Y Rattus norvegicus Norway rat  Rnor\Myo1g Y Rattus norvegicus Norway rat  Rnor\Myo1c Y Rattus norvegicus Norway rat  Rnor\Myo1d Y Rattus norvegicus Norway rat  Rnor\Myo1a Y Rattus norvegicus Norway rat  Rnor\Myo1b Y Homo sapiens Human  Hsap\MYO1G Y Homo sapiens Human  Hsap\MYO1A Y Homo sapiens Human  Hsap\MYO1H Y Homo sapiens Human  Hsap\MYO1C Y Homo sapiens Human  Hsap\ENSG00000128641 Y Homo sapiens Human  Hsap\MYO1D Y
Human Orthologs (6)
Gene OMIM HGNC Hsap\MYO1G     Hsap\MYO1A     Hsap\MYO1H     Hsap\MYO1C     Hsap\ENSG00000128641     Hsap\MYO1D
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM14400 Drosophila erecta Dere\GG14781 Drosophila yakuba Dyak\GE21145 Drosophila ananassae Dana\GF24454 Drosophila pseudoobscura pseudoobscura Dpse\GA21581 Drosophila willistoni Dwil\GK13346 Drosophila virilis Dvir\GJ12149 Drosophila mojavensis Dmoj\GI13054 Drosophila grimshawi Dgri\GH15215
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	41689 y1 v1; P{TRiP.HMS02253}attP2
Harvard	- PBac{PB}Myo61Fc03296
Kyoto	103985 y* w*; P{GawB}Myo61FNP1320 / TM6, P{UAS-lacZ.UW23-1}UW23-1 202159 y1 w67c23; P{GSV6}Myo61FGS9889/TM3, Sb1 Ser1
VDRC	v49345 w1118; P{GD4045}v49345
Genomic Clones ( 1 )
	BACR17K17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 66 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH04201
Other clones	FI19407
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH06064 GH13657 GH13670 GH23689 GH25605 GH25944 LP01830 LP05759 LP07757 LP08690 LP15886 LP18419 LP21301 LP21327 LP22223 LP23353 LP23903 RE06872 RE07809 RE14784 RE15961 RE19382 RE25452 RE27312 RE28639 RE30853 RE34718 RE39948 RE40611 RE43631 RE52194 RE59794 RE59994 RE60117 RE62031 RH10741 RH18152 RH18330 RH18714 RH18725 RH19656 RH57591
Other clones	79141 101435 105409 108063 214539.19 274424 522033 CK01057 EK228460 EK230443 EK234261 EK244030 EK301736 EN01232 EN12602 EN14302 EP11630 FGM233H11 GH18265 RP003121744 RP003343788 SD09960
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010246 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38153
Antibody Information
	polyclonal (Petzoldt et al., 2012, Morgan et al., 1995)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Myo61F has a role in maintaining the structural integrity of the brush border domain the larval midgut enterocyte and in providing resistance against oral infection by bacterial pathogens. (Hegan et al., 2007) Myo61F is required for normal left-right handedness of the embryonic gut. (Hozumi et al., 2006) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) The products of the Myo31DF and Myo61F loci are associated with the brush border cytoskeleton. (Morgan et al., 1995) Isolation and characterisation of Myo61F. (Morgan et al., 1994) Cloning and characterisation of Myo61F. (Cheney et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057586 NM_167870 NM_167871 NM_167872 RefSeq (Proteins) • NP_476934 NP_728594 NP_728595 NP_728596 DDBJ / EMBL / GenBank • AA140980 AAA19591 AAF47477 AAF47478 AAL39189 AAN11472 AAN11473 AC004489 AC005847 AI063969 AI135782 AI135790 AI257405 AI295145 AI404096 AI405567 AJ000879 AQ073914 AW940162 AY069044 CAA04367 L13070 U07596 Entrez Gene - A searchable database of RefSeq genes. • 38153 UniProtKB/Swiss-Prot • Q23979
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • IQ motif, EF-hand binding site (IPR000048) Myosin head, motor domain (IPR001609) Myosin tail 2 (IPR010926) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH04201
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0010246 DRSC - Results from RNAi screens. • FBgn0010246 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010246 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9155-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010246 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38153 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/myo1b_myo61f.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38153 modMine - Data generated by the modENCODE project. • FBgn0010246
Synonyms & Secondary IDs ( 26 )
Reported As
Symbol Synonym	CG9155 (Goldstein and Gunawardena, 2000, Huen, 2000.8.14, Rogers et al., 2003, Carrera et al., 2008, Wittkopp et al., 2008, Keleman et al., 2009.8.5, Petzoldt et al., 2012, Ni et al., 2010.12.1) Dm IB (Hodge and Cope, 2000) DmIB (Yamashita et al., 2000) DroMIB (Morgan, 1995, Morgan et al., 1994, Morgan et al., 1993) IB (Titus, 1997) MIB (Mermall et al., 2000) Myo1B (Oliver et al., 1999, Hegan et al., 2007, Rajan et al., 2009) MYO61F (Kravit, 1993) Myo61F (Wittkopp et al., 2006, Hozumi et al., 2006, Stuart et al., 2007, Baum, 2006, Taniguchi et al., 2007, Bauer et al., 2010, Hozumi et al., 2008, Carrera et al., 2008, Wittkopp et al., 2008, Otsuna and Ito, 2006, Kim et al., 2005, Wei et al., 2003) myo61f (Jia and Huan, 2010) myo61F (Ferjoux et al., 2007) Myo-IB (Berg et al., 2001) MYOIB (Goldstein and Gunawardena, 2000) MyoIC (Petzoldt et al., 2012) unnamed (Caggese, 1997.7.29)
Name Synonym	myosin 1b (Wolenski, 1995) myosin 1B (Rajan et al., 2009) myosin61F (Mann and Carroll, 2002) myosin 61F (Cheney et al., 1993) Myosin 61F   Myosin61F (Taniguchi et al., 2007, Hozumi et al., 2008) myosin I (Miller et al., 1991) myosin IB (Goldstein and Gunawardena, 2000, Zarnescu and Thomas, 1999, Rogers et al., 2003) myosin-IB (Morgan, 1994.3.8, Morgan, 1995, Morgan et al., 1995, Morgan et al., 1994) Myosin IB (Ragone et al., 1999, Rogers et al., 2003) Myosin-IB
Secondary FlyBase IDs
References ( 66 )
Generate a list of	All references relating to this gene ( 66 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 9 ) Review  ( 8 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 5 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 1 )
	Petzoldt et al., 2012, Development 139(10): 1874--1884 DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment. [FBrf0218056] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...