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Gene Dmel\Rbp9

General Information
Symbol	Dmel\Rbp9	Species	D. melanogaster
Name	RNA-binding protein 9	Annotation symbol	CG3151
Feature type	protein_coding_gene	FlyBase ID	FBgn0010263
Gene Model Status	Current	Stock availability	20 publicly available
Also Known As	fs(2)B, fes, RRM9, RRM10
Genomic Location
Chromosome (arm)	2L	Recombination map	2-5
Cytogenetic map	23C3-23C3	Sequence location	2L:2,954,762..2,968,434 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene RNA-binding protein 9 is referred to in FlyBase by the symbol Dmel\Rbp9 (CG3151, FBgn0010263). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: mRNA binding. There is experimental evidence that it is involved in the biological process: establishment of blood-brain barrier. 28 alleles are reported. The phenotypes of these alleles are annotated with: cystocyte; ovariole; fusome; mesothoracic tergum; egg chamber; germarium; nurse cell. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; Paraneoplastic encephalomyelitis antigen; RNA recognition motif domain; Splicing factor ELAV/HuD. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval midgut, larval/adult hindgut, adult heart, adult male reproductive system. Gene sequence location is 2L:2954762..2968434.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	fs(2)B: female sterile (2) of Bridges External morphology and sexual behavior normal (Burnet, Connolly, Kearney, and Cook, 1973, J. Insect Physiol. 19: 2421-31). Males fertile; females sterile. After rearing on glucose diet mutant females are less receptive to copulation attempts and more vigorously courted than wild-type virgins (Cook and Connolly, 1976, J. Insect Physiol. 22: 1727-35). Germaria of ovaries of homozygous females larger than normal; cysts contain 3 times the normal number of cells; metaphase figures found throughout germarium. Just over half the oogonia undergo complete cleavage with the loss of ring canal connections to other cells; remaining cells in small clusters with tenuous connections (Johnson and King, 1972, Biol. Bull. 143: 525-47). Vitellaria subdivided into a series of sausage-shaped cell aggregates, each surrounded with an ill-defined follicular epithelium and filled with hundreds to thousands of mitotically active oogonia-like cells [King, Burnett, and Staley, 1957, Growth 21: 239-61 (fig.)]. These cells occasionally differentiate into cells resembling nurse cells, which may have polytene chromosomes, and rarely into oocytes. fs(2)B ovaries transplanted into wild-type hosts in late larval stages and reciprocal transplants develop autonomously (Clancy and Beadle, 1937, Biol. Bull. 72: 47-56; Klug, Bodenstein, and King, 1968, J. Exp. Zool. 167: 151-56). The cells of the corpus-allatum corpus-cardiacum complex of homozygous fs(2)B females appear to be prevented from releasing their hormone products and undergo degenerative changes; these effects reversable by vitellogenic activity of wild-type ovary implanted into the abdomen of the fs(2)B female (King, Aggarwal, and Bodenstein, 1966, J. Exp. Zool. 161: 151-76; Aggarwal and King, 1971, J. Morphol. 134: 437-46). Abdominal fat cells of fs(2)B females 1.5 times size of those in wild type. They resemble male fat cells in having more fat and less glycogen than those of normal females; return to normal size and composition following implantation of wild-type ovary (Butterworth and Bodenstein, 1968, J. Exp. Zool. 167: 207-18). fs(2)B ovaries show reduced levels of thymidylate synthetase (Carpenter, 1973, Genetics 75: 113-22). RK3.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features HFA00416 BKN29093 HFA00779
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 23C3-23C3   Limits computationally determined from genome sequence between P{PZ}lilli00632 and P{PZ}toc01361
Experimentally Determined Cytological Location
Cytogenetic map Notes References 23C-23C     (Kim, 1993.4.12) 22E1-23D6   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-5
Left of (cM)
Right of (cM)	Rrp1 (Huang et al., 1998)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rbp9 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	3' UTRs contain an ORF that is conserved among Drosophila species; potential peptide 154 aa in length; initial methionine is not conserved; potential stop-codon readthrough.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rbp9-RB FBtr0077637  NM_134297   3405   444 Rbp9-RC FBtr0077639  NM_134300   3425   444 Rbp9-RE FBtr0077638  NM_134298   3390   439 Rbp9-RF FBtr0077640  NM_134299   3410   439 Rbp9-RG FBtr0100468  NM_001014462   6973   439 Rbp9-RH FBtr0290237  NM_057588   3478   439 Rbp9-RI FBtr0290238  NM_057589   3493   444
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rbp9-PB   FBpp0077322   46.8   444   9.22     NP_599124   AAF51177 Rbp9-PC   FBpp0077324   46.8   444   9.22     NP_599127   AAF51178 Rbp9-PE   FBpp0077323   46.2   439   9.53     NP_599125   AAN10402 Rbp9-PF   FBpp0077325   46.2   439   9.53     NP_599126   AAN10403 Rbp9-PG   FBpp0099898   46.2   439   9.53     NP_001014462   AAX52651 Rbp9-PH   FBpp0288676   46.2   439   9.53     NP_476936   AAN10401 Rbp9-PI   FBpp0288677   46.8   444   9.22     NP_476937   AAF51179
Additional Polypeptide Data & Comments
Reported size (kDa)	444, 439 (aa); 50 (kD observed) (Kim and Baker, 1993)
Comments	Antibodies against Rbp9 protein recognize both forms of the protein. (Kim and Baker, 1993) Rbp9-2 (FlyBase, 1992-) Rbp9-1 (FlyBase, 1992-)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Paraneoplastic encephalomyelitis antigen (IPR002343) Splicing factor ELAV/HuD (IPR006548) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA538943   AE014134 AAF51177   AE014134 AAF51178   AE014134 AAF51179   AE014134 AAN10401   AE014134 AAN10402   AE014134 AAN10403   AE014134 AAX52651   AC007765   AC010009   AC092187   AI108697   AI386763   AW940279   AX093902   AY060253 AAL25292   BH146180   BH146181   BI566188   BI566908   BI569858   BI570104   BI583157   BI589266   BI589760   BI634136   BP554577   BT011197   BT029936   CO179516   CO186518   CO186639   CO189287   CO196542   CO262068   CO262389   CO262995   CO266866   CO268154   CO268296   CO268644   CO268926   CO270756   CO274025   CO274219   CO274410   CO274673   CO274755   CO291320   CO294828   CO297948   CO301716   CO304334   CO306500   CO309936   CO313693   CO314680   CO315122   CO315176   CO322694   CO331508   CO332259   CO341219   CZ471678   CZ471679   CZ473798   CZ476159   CZ477263   CZ477887   CZ479994   EC059822   EC060553   EC063109   EC064746   EC065568   EC071071   EC071133   EC080527   EC085718   EC088426   EC090447   EC090501   EC196916   EC197322   EC208327   EC209443   EC210265   EC213982   EC214241   EC216603   EC217656   EC217703   EC219539   EC220588   EC221885   EC224511   EC225727   EC227124   EC230956   EC230957   EC232647   EC238060   EC242486   EC242487   EC242621   EC243980   EC244001   EC244094   EC247357   EC248856   EC252152   EL872653   EL872654   EL872773   EL872774   EL872894   EL878617   EL878618   EL878797   L04930 AAC13645   L04930 AAC13646   S51727 AAB24633   S51738 AAB24630   S55886 AAB25519
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24474 Q26278 Q26279 Q26293 Q95TA9 Q9VQJ0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
expression microarray Stage Tissue/Position (including subcellular localization) Reference adult stage   eye   (Xu et al., 2004) northern blot Stage Tissue/Position (including subcellular localization) Reference third instar larval stage     (Kim and Baker, 1993) pupal stage & adult stage     (Kim and Baker, 1993)
Additional Descriptive Data
	Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads. (Xu et al., 2004)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference pupal stage | late   cell body of adult brain   (Kim and Baker, 1993) pupal stage & adult stage     (Kim and Baker, 1993) adult stage   cell body of lamina   (Kim and Baker, 1993) thoracico-abdominal ganglion   (Kim and Baker, 1993) antenna   (Kim and Baker, 1993) cell body of adult brain   (Kim and Baker, 1993)
Additional Descriptive Data
	Rbp9 protein is localized to nerve cell bodies of the CNS in cryosections from pupae and adults. The nuclei of the brain and thoracic ganglion are the most heavily stained. Low levels are detected in the lamina cell body layer and antennae. (Kim and Baker, 1993)
Marker for
Subcellular Localization
CV Term	nucleus (Kim and Baker, 1993)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0010263    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0010263 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during late pupal stages, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0010263 expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP    3728 pupae 3d postWPP    3387 pupae 4d postWPP    2519 adult male 01day    3238 adult male 05day    3167 adult male 30day    2954 adult female 01day    1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr  (142) embryo 12-14hr  (250) embryo 14-16hr  (362) embryo 16-18hr  (479) embryo 18-20hr  (456) embryo 20-22hr  (381) embryo 22-24hr  (418) larva L1  (526) larva L2  (388) larva L3 12hr old  (857) larva L3 puffstage 1-2  (856) larva L3 puffstage 3-6  (988) larva L3 puffstage 7-9  (800) white prepupae new  (597) white prepupae 12hr  (658) white prepupae 24hr  (1151) pupae 2d postWPP  (3728) pupae 3d postWPP  (3387) pupae 4d postWPP  (2519) adult male 01day  (3238) adult male 05day  (3167) adult male 30day  (2954) adult female 01day  (1657) adult female 05day  (497) adult female 30day  (498) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP  (3728) pupae 3d postWPP  (3387) pupae 4d postWPP  (2519) adult male 01day  (3238) adult male 05day  (3167) adult male 30day  (2954) adult female 01day  (1657) adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP    3728 pupae 3d postWPP    3387 pupae 4d postWPP    2519 adult male 01day    3238 adult male 05day    3167 adult male 30day    2954 adult female 01day    1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP    3728 pupae 3d postWPP    3387 pupae 4d postWPP    2519 adult male 01day    3238 adult male 05day    3167 adult male 30day    2954 adult female 01day    1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0010263 expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP    3728 pupae 3d postWPP    3387 pupae 4d postWPP    2519 adult male 01day    3238 adult male 05day    3167 adult male 30day    2954 adult female 01day    1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr  142 embryo 12-14hr  (250) embryo 14-16hr  (362) embryo 16-18hr  (479) embryo 18-20hr  (456) embryo 20-22hr  (381) embryo 22-24hr  (418) larva L1  (526) larva L2  (388) larva L3 12hr old  (857) larva L3 puffstage 1-2  (856) larva L3 puffstage 3-6  (988) larva L3 puffstage 7-9  (800) white prepupae new  (597) white prepupae 12hr  (658) white prepupae 24hr  (1151) pupae 2d postWPP  (3728) pupae 3d postWPP  (3387) pupae 4d postWPP  (2519) adult male 01day  (3238) adult male 05day  (3167) adult male 30day  (2954) adult female 01day  (1657) adult female 05day  (497) adult female 30day  (498) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP  (3728) pupae 3d postWPP  (3387) pupae 4d postWPP  (2519) adult male 01day  (3238) adult male 05day  (3167) adult male 30day  (2954) adult female 01day  1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP    3728 pupae 3d postWPP    3387 pupae 4d postWPP    2519 adult male 01day    3238 adult male 05day    3167 adult male 30day    2954 adult female 01day    1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    39 embryo 04-06hr    10 embryo 06-08hr    22 embryo 08-10hr    43 embryo 10-12hr    142 embryo 12-14hr    250 embryo 14-16hr    362 embryo 16-18hr    479 embryo 18-20hr    456 embryo 20-22hr    381 embryo 22-24hr    418 larva L1    526 larva L2    388 larva L3 12hr old    857 larva L3 puffstage 1-2    856 larva L3 puffstage 3-6    988 larva L3 puffstage 7-9    800 white prepupae new    597 white prepupae 12hr    658 white prepupae 24hr    1151 pupae 2d postWPP    3728 pupae 3d postWPP    3387 pupae 4d postWPP    2519 adult male 01day    3238 adult male 05day    3167 adult male 30day    2954 adult female 01day    1657 adult female 05day    497 adult female 30day    498 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0010263    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0010263 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval midgut, larval/adult hindgut, adult heart, adult male reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_10
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010263 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CT35452;CG3151-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 Rbp9GD10278 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Rbp9GD10278 viable Rbp9k12901 Rbp9P2398 Rbp9P2690 Rbp9P2775 Sterility Allele female sterile Rbp9P2690 Rbp9P2775 Rbp9Δ1 Rbp9Δ2 Rbp9Δ3 Rbp9Δ5 female sterile | partially Rbp9P2398 Rbp9P2567 female sterile | recessive Rbp9B Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 Rbp9GD10278 locomotor behavior defective | conditional Rbp9P2690 short lived Rbp9P2690 visible, with Scer\GAL4pnr-MD237 Rbp9GD10278 wild-type Rbp9+t17.1 Phenotype manifest in Allele cystocyte Rbp9Δ1 Rbp9P2690 Rbp9P2775 egg chamber Rbp9Δ1 Rbp9P2567 Rbp9P2398 Rbp9P2690 Rbp9P2775 Rbp9Δ105 fusome Rbp9Δ1 germarium Rbp9Δ1 Rbp9P2690 Rbp9P2775 mesothoracic tergum, with Scer\GAL4pnr-MD237 Rbp9GD10278 nurse cell Rbp9P2567 Rbp9P2398 ovariole Rbp9Δ1 Rbp9P2690 Rbp9P2775
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of Rbp9 Class Mutagen Stocks Known lesion Rbp9B spontaneous 8 -- Rbp9BG02784 P-element activity 1 -- Rbp9d04230 P-element activity 1 -- Rbp9d10441 P-element activity 1 -- Rbp9e02166 piggyBac transposase 1 -- Rbp9P2567 loss of function allele, hypomorphic allele - genetic evidence 1 -- Rbp9P2690 loss of function allele Delta2-3 1 Yes Rbp9P2775 hypomorphic allele - genetic evidence Delta2-3 1 Yes Rbp9Δ1 loss of function allele Delta2-3 1 Yes Rbp9C144 0 Yes Rbp9d00586 P-element activity 0 -- Rbp9d08275 P-element activity 0 -- Rbp9d11732 P-element activity 0 -- Rbp9k12901 P-element activity 0 Yes Rbp9P1374 Delta2-3 0 Yes Rbp9P2398 hypomorphic allele - genetic evidence Delta2-3 0 Yes Rbp9Δ105 Delta2-3 0 Yes Rbp9Δ106 Delta2-3 0 Yes Rbp9Δ123 Delta2-3 0 Yes Rbp9Δ2 Delta2-3 0 Yes Rbp9Δ3 Delta2-3 0 Yes Rbp9Δ56 Delta2-3 0 Yes Rbp9Δ5 Delta2-3 0 Yes Rbp9Δ64 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rbp9 Class Mutagen Stocks Known lesion Rbp9GD10278 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Rbp9JF03084 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rbp9KK109093 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rbp9+t17.1 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(2L)C144 (Cook et al., 2010.1.8)
Disrupted in	Df(2L)16X42 (Kim-Ha et al., 1999)
Not disrupted in	Df(2L)3G (Vázquez et al., 2008) Df(2L)JS17 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10278} NA P{KK109093} NA P{TRiP.JF03084} NA characterization construct P{elav::Rbp9ER1} NA P{elav::Rbp9ER3} NA P{elav::Rbp9ER12} NA P{Rbp9.17.1.K} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}Rbp9BG02784 NA PBac{RB}Rbp9e02166 NA insertion of enhancer trap P{lacW}l(2)SH1110SH1110 No P{lacW}Rbp9k12901 No P{lacW}Rbp9P1374 No P{lacW}Rbp9P2398 No P{lacW}Rbp9P2567 No P{lacW}Rbp9P2690 No P{lacW}Rbp9P2775 No insertion of mobile activating element P{XP}Rbp9d00586 NA P{XP}Rbp9d00587 NA P{XP}Rbp9d04230 NA P{XP}Rbp9d08275 NA P{XP}Rbp9d10441 NA P{XP}Rbp9d11732 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
establishment of blood-brain barrier inferred from mutant phenotype (Kim et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
mRNA 3'-UTR binding traceable author statement (Deng and Lin, 2001) mRNA binding inferred from sequence or structural similarity (Lasko, 2000) non-traceable author statement (Kim, 1993.3.10, Kim, 1993.3.10, Kim, 1993.4.12, Good, 1997) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-) RNA binding inferred from electronic annotation with InterPro:IPR002343, InterPro:IPR006548 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
germarium-derived egg chamber formation traceable author statement (Kim et al., 2002)
Cellular Component
CV term Evidence References
nucleus traceable author statement (Good, 1997)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rbp9 allele Gene References Rbp9B cup (Keyes and Spradling, 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59718 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010263 DroID - A comprehensive database of gene and protein interactions. • FBgn0010263 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33498
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14992 Dere\GG24901 Dgri\GH13638 Dmoj\GI11879 Dper\GL26299 Dpse\GA28809 Dsec\GM18380 Dsim\GD23195 Dvir\GJ13991 Dwil\GK14865 Dyak\GE18193
Curated drosophilid orthologs	Dpse\GA16290
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_001951393.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000028031 Bos taurus (bovine) CN03688 Caenorhabditis brenneri (small nematode) CE00983 Caenorhabditis elegans (worm) ENSCAFP00000002458 Canis familiaris (dog) ENSCINP00000009777 Ciona intestinalis (sea squirt) JA03690 Caenorhabditis japonica (nematode) ENSCPOP00000009403 Cavia porcellus (guinea pig) RP16272 Caenorhabditis remanei (nematode) ENSCSAVP00000005772 Ciona savignyi (sedentary tunicate) 172701 Capitella spl (polychaete worm) DDB0233759 Dictyostelium discoideum (amoeba) 98025 Daphnia pulex (daphnia) ENSDARP00000006457 Danio rerio (zebra fish) ENSDARP00000032015 Danio rerio (zebra fish) ENSECAP00000017957 Equus caballus (horse) ENSGACP00000025756 Gymnopilus aculeatus (mushroom) ENSGALP00000025429 Gallus gallus (chicken) 187316 Hypsipyla robusta (cedar shoot borer) ENSP00000352612 Homo sapiens (human) 25688 Monocosta brevicollis (choanoflagellate) ENSMODP00000004833 Monodelphis domestica (gray opossum) ENSMMUP00000013125 Macaca mulatta (rhesus monkey) ENSMUSP00000102733 Mus musculus (house mouse) 214798 Nematostella vectensis (starlet anemone) ENSOANP00000015299 Ornithorynchus anatinus (platypus) PP19260 Pristionchus pacificus (nematode) ENSPPYP00000021474 Pongo pygmaeus (orangutan) ENSPTRP00000035622 Pan troglodytes (chimpanzee) ENSRNOP00000009035 Rattus norvegicus (norway rat) Smp_194950 Schistosoma mansoni (trematode parasite) ENSTNIP00000014253 Tetraodon nigroviridis (green spotted pufferfish) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0235624 FBgn0173287 FBgn0117030
Stocks & Reagents
Stocks Listed in FlyBase ( 20 )
Bloomington	25775 w*; Rbp9Δ1/CyO 12836 w1118; P{GT1}Rbp9BG02784 25776 w*; P{lacW}Rbp9P2567/CyO 25777 w*; P{lacW}Rbp9P2775/CyO 25778 w*; P{lacW}Rbp9P2690/CyO 303 Rbp9B Alu1 lt1/SM5
Harvard	- P{XP}Rbp9d04230 - P{XP}Rbp9d10441 - PBac{RB}Rbp9e02166
VDRC	v101412 P{KK109093}VIE-260B v33938 w1118; P{GD10278}v33938 v33939 w1118; P{GD10278}v33939
	More stocks available...
Genomic Clones ( 1 )
	BACR23N06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 57 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH07919 GH26440
Other clones	DPiM_GH26440 IP15810
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH16969 LD18621 RH02027 RH02356 RH03244 RH21680 RH31646 RH35362 RH36705 RH37345
Other clones	77568 100490 119342 EK005502 EK020601 EK021734 EK043802 EK059606 EK077627 EK084322 EK085227 EK087020 EK090352 EK090852 EK092218 EK093110 EK100140 EK112925 EK113559 EK114033 EK115028 EK115348 EK147808 EK171002 EK181945 EK188651 EK207808 EK220002 EK233748 EK237426 EK239161 EK239562 EK244362 EK251245 EK300016 EK302939 EP12050 FGM230F03 IP04538 IP04638 IP04738 IP08450 IP08650
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010263 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33498
Antibody Information
	polyclonal (Kim and Baker, 1993)
Other Information
Discoverer
	Bridges, 25th March 1929.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rbp9 CG5808 (Kodira, 1999.11)
Source for database merge of	Source for merge of: Rbp9 CG15402 (FlyBase Genome Annotators, 2002-2003) Source for merge of: Rbp9 fs(2)B (Donnelly et al., 2004) Source for merge of: Rbp10 CG3151 (Misra, 2000.7.28)
Additional comments	Annotations CG3151 and CG15402 merged as CG3151 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	Area matching Drosophila rbp9 gene, Acc. No. S55886. (Davies, 2001.3.15) Rbp9 is required during the cystocyte differentiation stage in females for the maintenance of germline sexual identity. (Lee et al., 2000) Rbp9 protein binds bam mRNA to down-regulate bam protein expression. The concentrated localization of Rbp9 protein in the oocyte of the early egg chambers may be required for proper oocyte determination or positioning. (Kim-Ha et al., 1999) Rbp9 stimulates cystocyte differentiation by regulating the expression of bam. (Kim-Ha et al., 1999) Mutants show a female sterility phenotype, due to defective oogenesis, rather than neuronal defects. (Kim-Ha et al., 1999) The consensus Rbp9 binding site, UUUXUUUU, is present in two copies in the 3' untranslated region of emc mRNA. UV crosslinking demonstrates a specific interaction between the two, and Northern analysis revealed that Rbp9 functions as a regulator of mRNA stability. (Park et al., 1998) Mutants display germline hyperplastic phenotype. (Mechler, 1994) Rbp9 is molecularly characterised and expression pattern determined. Results suggest Rbp9 may be involved in the development of the nervous system perhaps by acting as a regulator of nervous system-specific RNA processing decisions. (Kim and Baker, 1993) A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs. (Kim and Baker, 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057588 NM_057589 NM_134297 NM_134298 NM_134299 NM_134300 NM_001014462 RefSeq (Proteins) • NP_476936 NP_476937 NP_599124 NP_599125 NP_599126 NP_599127 NP_001014462 DDBJ / EMBL / GenBank • AAB24630 AAB24633 AAB25519 AAC13645 AAC13646 AAF51177 AAF51177.2 AAF51178 AAF51178.2 AAF51179 AAF51179.2 AAF51179.3 AAL25292 AAN10401 AAN10401.1 AAN10401.2 AAN10402 AAN10402.1 AAN10403 AAN10403.1 AAX52651 AC007765 AC010009 AC092187 AF073994 AI108697 AW940279 AX093902 AY060253 BH146180 BH146181 CZ471678 CZ471679 CZ473798 CZ476159 CZ477263 CZ477887 CZ479994 L04930 S51727 S51738 S55886 Entrez Gene - A searchable database of RefSeq genes. • 33498 UniProtKB/TrEMBL • Q9VQJ0 Q95TA9 Q24474 Q26278 Q26279 Q26293
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Paraneoplastic encephalomyelitis antigen (IPR002343) Splicing factor ELAV/HuD (IPR006548) Nucleotide-binding, alpha-beta plait (IPR012677)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59718 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010263 DroID - A comprehensive database of gene and protein interactions. • FBgn0010263 DRSC - Results from RNAi screens. • FBgn0010263 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010263 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CT35452;CG3151-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010263 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33498 InParanoid A subset of ortholog calls from InParanoid. • XP_001951393.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000028031 Bos taurus (bovine) CN03688 Caenorhabditis brenneri (small nematode) CE00983 Caenorhabditis elegans (worm) ENSCAFP00000002458 Canis familiaris (dog) ENSCINP00000009777 Ciona intestinalis (sea squirt) JA03690 Caenorhabditis japonica (nematode) ENSCPOP00000009403 Cavia porcellus (guinea pig) RP16272 Caenorhabditis remanei (nematode) ENSCSAVP00000005772 Ciona savignyi (sedentary tunicate) 172701 Capitella spl (polychaete worm) DDB0233759 Dictyostelium discoideum (amoeba) 98025 Daphnia pulex (daphnia) ENSDARP00000006457 Danio rerio (zebra fish) ENSDARP00000032015 Danio rerio (zebra fish) ENSECAP00000017957 Equus caballus (horse) ENSGACP00000025756 Gymnopilus aculeatus (mushroom) ENSGALP00000025429 Gallus gallus (chicken) 187316 Hypsipyla robusta (cedar shoot borer) ENSP00000352612 Homo sapiens (human) 25688 Monocosta brevicollis (choanoflagellate) ENSMODP00000004833 Monodelphis domestica (gray opossum) ENSMMUP00000013125 Macaca mulatta (rhesus monkey) ENSMUSP00000102733 Mus musculus (house mouse) 214798 Nematostella vectensis (starlet anemone) ENSOANP00000015299 Ornithorynchus anatinus (platypus) PP19260 Pristionchus pacificus (nematode) ENSPPYP00000021474 Pongo pygmaeus (orangutan) ENSPTRP00000035622 Pan troglodytes (chimpanzee) ENSRNOP00000009035 Rattus norvegicus (norway rat) Smp_194950 Schistosoma mansoni (trematode parasite) ENSTNIP00000014253 Tetraodon nigroviridis (green spotted pufferfish) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33498 modMine - Data generated by the modENCODE project. • FBgn0010263 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0235624 FBgn0173287 FBgn0117030
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	CG3151 (Spellman and Rubin, 2002, Keleman et al., 2009.8.5, Perkins et al., 2009, Gandhi et al., 2007) CG15402 (Spellman and Rubin, 2002) fes (Mulligan, 2003, Berg, 1994, Gateff, 1978, ) fs(2)B (Kai et al., 2005, de Cuevas et al., 1997, Watson et al., 1994, Sharakhov et al., 1997, Malceva and Zhimulev, 1993, Gateff and Mechler, 1989, Keyes and Spradling, 1997, Mechler, 1994, Donnelly et al., 2004, Flatt et al., 2005, Gigliotti et al., 2000) rbp (Tchurikov et al., 1998) rbp9 (Good, 1997, Wittwer et al., 2001, Mount, 2000.4.7, Kim, 1993.4.12, Ghabrial et al., 1998, Kim and Baker, 1993, Yao et al., 1993, Samson, 2008, Tastan et al., 2010) RBP9 (Tsoi, 1998.6.19, Kim, 1992.11.20, Soller et al., 2010) Rbp9 (Kim-Ha, 1999.12.2, Spellman and Rubin, 2002, Vanzo et al., 2007, Kim-Ha, 2000, Toba and White, 2008, Cook et al., 2010.1.8, Kim et al., 2010) RBP9-2 (Hughes, 1999) rbp9a (Tchurikov et al., 1998) Rbp10   Rpb9 (Vázquez et al., 2008) RRM9 (Kim, 1993.3.10, ) rrm9 (Kim and Baker, 1993) RRM10 (Kim, 1993.3.10, ) rrm10 (Kim and Baker, 1993)
Name Synonym	female sterile (Berg, 1994) female sterile(2)Bridges (Berg, 1994, ) female sterile (2) Bridges (Flatt et al., 2005) RNA-binding protein 9   RNA binding protein 9 (Samson, 2008) RNA-binding protein 10
Secondary FlyBase IDs
	FBgn0010253 FBgn0031482
References ( 97 )
Generate a list of	All references relating to this gene ( 97 )
List References by type	Research paper  ( 41 ) Supplementary material  ( 5 ) Review  ( 11 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 11 ) FlyBase analysis  ( 2 ) Biography  ( 1 ) DNA/RNA sequence record  ( 6 ) Curated genome annotation  ( 3 ) Patent  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 3 )
	Kim et al., 2010, Mol. Cells 29(1): 93--98 Blood-brain barrier defects associated with Rbp9 mutation. [FBrf0210543] Soller et al., 2010, Biochem. Soc. Trans. 38(4): 1122--1124 Determinants of ELAV gene-specific regulation. [FBrf0211339] Tastan et al., 2010, Development 137(19): 3167--3176 Drosophila Ataxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts. [FBrf0211752] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...