FlyBase Gene Report: Dmel\RpS13

General Information

Symbol

Dmel\RpS13

Species

D. melanogaster

Name

Ribosomal protein S13

Annotation Symbol

CG13389

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010265

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Ribosomal protein S13 (RpS13) encodes a structural constituent of ribosomes. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

M(2)32A, l(2)k09614, Minute(2)32A, S13

Key Links

Genomic Location

Cytogenetic map

29B2-29B2

Sequence location

2L:8,363,477..8,364,662 [-]

Recombination map

2-31

RefSeq locus

NT_033779 REGION:8363477..8364662

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables small ribosomal subunit rRNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000223267

(assigned by GO_Central )

(Gaudet et al., 2011)

enables structural constituent of ribosome

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000223267

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

traceable author statement

(Marygold et al., 2007)

inferred by curator from GO:0022626

(Anger et al., 2013, Alonso and Santaren, 2006)

involved_in translation

inferred from electronic annotation with InterPro:IPR000589, InterPro:IPR012606

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Rugjee et al., 2013)

located_in cytosolic ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

colocalizes_with nuclear chromosome

inferred from direct assay

(Rugjee et al., 2013)

located_in nucleolus

inferred from direct assay

(Rugjee et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of cytosolic small ribosomal subunit

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000223267

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleolus

inferred from biological aspect of ancestor with PANTHER:PTN000223268

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS

Protein Family (UniProt)

Belongs to the universal ribosomal protein uS15 family. (Q03334)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Ribosomal protein S13 (RpS13) encodes a structural constituent of ribosomes. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpS13 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q03334)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS13-RA

FBtr0079724

NM_057590

633

151

RpS13-RB

FBtr0100541

NM_001038796

611

151

RpS13-RC

FBtr0346454

NM_001298818

899

151

Additional Transcript Data and Comments

Reported size (kB)

0.6 (northern blot)

(Saeboe-Larssen and Lambertsson, 1996)

0.74 (northern blot)

(McNabb and Ashburner, 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RpS13-PA

FBpp0079328

17.2

151

11.24

FBpp0099990

17.2

151

11.24

FBpp0312104

17.2

151

11.24

Q03334

NP_001285747

AHN54261

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

151 aa isoforms: RpS13-PA, RpS13-PB, RpS13-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

151 (aa); 17 (kD predicted)

(Saeboe-Larssen and Lambertsson, 1996)

151 (aa); 17 (kD)

(McNabb and Ashburner, 1993)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS13 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS13_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Saeboe-Larssen and Lambertsson, 1996, McNabb and Ashburner, 1993)

Additional Descriptive Data

RpS13 transcripts are abundantly expressed at all stages of development. They are three times more abundant in females than males.

(Saeboe-Larssen and Lambertsson, 1996)

RpS13 transcripts are expressed at all stages of development

(McNabb and Ashburner, 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Rugjee et al., 2013)

located_in cytosolic ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

colocalizes_with nuclear chromosome

inferred from direct assay

(Rugjee et al., 2013)

located_in nucleolus

inferred from direct assay

(Rugjee et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS13 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS13

View exonic expression by tissue for Dmel\RpS13

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010265

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34149/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010265

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010265

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010265

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13389

Flygut - An atlas of the Drosophila adult midgut

FBgn0010265

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS13

Transgenic constructs containing regulatory region of RpS13

Aberrations (Deficiencies and Duplications) ( 12 )

Inferred from experimentation ( 12 )

Gene disrupted in

Df(2L)BSC53

(Marygold et al., 2007)

Df(2L)dp^h27

(Saeboe-Larssen and Lambertsson, 1996)

Df(2L)prd2.27

(Saeboe-Larssen and Lambertsson, 1996)

Gene not disrupted in

Df(2L)J2

(Saeboe-Larssen and Lambertsson, 1996)

Df(2L)J27

(Saeboe-Larssen and Lambertsson, 1996)

Df(2L)N22-5

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)N22-14

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)Prl

(Saeboe-Larssen and Lambertsson, 1996)

Df(2L)TE29Aa-11

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)TE29Aa-14

(BDGP Project Members, 1994-1999)

Gene duplicated in

Dp(2;3)dp^h27

(Saeboe-Larssen and Lambertsson, 1996)

Dp(2;3)prd^2.27

(Saeboe-Larssen and Lambertsson, 1996)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^tin.CΔ4

RpS13^GD8007

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

RpS13^GD8007

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

RpS13^GD8007

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpS13^GD8007

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^amn-c651

RpS13^{dsRNA.WIZ.UAS}

abnormal size | adult stage

RpS13¹

increased cell death | larval stage

RpS13¹

Minute

Minute | dominant

RpS13¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

RpS13^GD8007

visible

RpS13¹

visible | dominant

RpS13^unspecified

Phenotype manifest in

Allele

adult heart

RpS13¹

imaginal disc | somatic clone

RpS13¹

macrochaeta

prothoracic gland, with Scer\GAL4^amn-c651

RpS13^{dsRNA.WIZ.UAS}

scutellar bristle

RpS13¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RPS13

14 of 15

Yes

Hsap\MRPS15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rps13

9 of 15

Yes

Mmus\Rps13-ps5

5 of 15

Yes

Mmus\Mrps15

1 of 15

Rattus norvegicus (Norway rat) (4)

Rnor\LOC684988

11 of 13

Yes

Rnor\LOC683961

3 of 13

Yes

Rnor\Rps13

3 of 13

Yes

Rnor\Mrps15

1 of 13

Xenopus tropicalis (Western clawed frog) (2)

Xtro\rps13

8 of 12

Yes

Xtro\mrps15

1 of 12

Danio rerio (Zebrafish) (1)

Drer\rps13

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\rps-13

14 of 15

Yes

Cele\mrps-15

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\AT3G60770

9 of 9

Yes

Atha\RPS13A

9 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\RPS13

14 of 15

Yes

Scer\MRPS28

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rps13

11 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

cardiomyopathy (postulated), translation-critical genes

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

RpS13¹

model of intrinsic cardiomyopathy

CEA

(Casad et al., 2011)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS13; ribosomal protein S13

14 of 15

180476

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS13 - Cnb

anti tag coimmunoprecipitation, peptide massfingerprinting

(Januschke et al., 2013)

RpS13 - InR

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS13 - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL37a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

RpS13 - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS13 - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS13 - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS13 - DENR

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Schleich et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pi3K92E

enhanceable

RpS13

(Coelho et al., 2005)

Polr1B

suppressible

RpS13

(Grewal et al., 2005)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60275

DroID - A comprehensive database of gene and protein interactions.

FBgn0010265

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS13

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0010265

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-31

Cytogenetic map

29B2-29B2

Sequence location

2L:8,363,477..8,364,662 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

29B2-29B2

Limits computationally determined from genome sequence between P{EP}EP646^EP646&P{lacW}RpS13^k09614 and P{lacW}l(2)k12914^k12914&P{lacW}Akap200^k07118a

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32A-32A

(Saeboe-Larsson, 1995.9.26)

29A-29A

(Ashburner, 1992.12.9)

29B1-29B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

32A-32A

(determined by in situ hybridisation)

(Saeboe-Larssen and Lambertsson, 1996)

29B1-29B2

(BDGP Project Members, 1994-1999)

29A-29A

(determined by in situ hybridisation)

(McNabb and Ashburner, 1993)

Experimentally Determined Recombination Data

Location

2-31

(FlyBase, 2016)

2-27.7

(Comeron et al., 2012)

2-42

(Saeboe-Larssen and Lambertsson, 1996)

(Saeboe-Larsson, 1995.9.26)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

79222

w¹¹¹⁸; P{UAS-RpS13.FLAG}2

33275

w¹¹¹⁸; P{EP}RpS13^G8009/SM6a

2246

y¹ w^*; P{lacW}RpS13¹/CyO

34820

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00135}attP2

FlyORF

F001572

M{UAS-RpS13.ORF}ZH-86Fb

F000797

M{UAS-RpS13.ORF.3xHA}ZH-86Fb

F003137

M{UAS-RpS13.ORF.3xHA.GW}ZH-86Fb

Kyoto

103664

w^*; P{GawB}RpS13^NP0590 / CyO

102720

y¹ w^67c23 ; P{lacW}RpS13^k09614 / CyO

VDRC

v105496

P{KK109083}VIE-260B

v17168

w¹¹¹⁸; P{GD8007}v17168

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (365)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD23958

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010265

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010265

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34149

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: RpS13 l(2)k09614

(Spradling et al., 1999)

Additional comments

Other Comments

Host gene for maternally inherited stable intronic sequence RNA (sisRNA).

(Tay and Pek, 2017)

Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.

(Pek et al., 2015)

Haploinsufficient locus.

(Cook et al., 2012)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Minute gene.

(Marygold et al., 2007)

Deletions removing RpS13 but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

(Marygold et al., 2007)

Molecularly-defined mutations in RpS13 result in Minute phenotypes.

(Marygold et al., 2007)

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

cDNA for ribosomal protein S13 isolated.

(McNabb and Ashburner, 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 66 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34149

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q03334

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q03334

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2J950

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q03334

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010265

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010265

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34149/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010265

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpS13_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010265

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010265

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13389

Flygut - An atlas of the Drosophila adult midgut

FBgn0010265

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0010265

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34149

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004990

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG13389

MARRVEL_MODEL - MARRVEL (model organism gene)

34149

modMine - A data warehouse for the modENCODE project

FBgn0010265

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

10910-RpS13[k09614]

BioGRID - A database of protein and genetic interactions.

60275

DroID - A comprehensive database of gene and protein interactions.

FBgn0010265

DRSC - Results frm RNAi screens

FBgn0010265

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010265

FlyMine - An integrated database for Drosophila genomics

FBgn0010265

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS13

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG13389

(Kolkhof et al., 2017, Tay and Pek, 2017, Pek et al., 2015, Schertel et al., 2013, Jaspers et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Piacentini et al., 2009, Goshima et al., 2007, Marygold et al., 2007, Cermelli et al., 2006)

M(2)29B

(Marygold et al., 2007)

M(2)32

(Lambertsson et al., 1994)

M(2)32A

(Rugjee et al., 2013, Grewal et al., 2005, Montagne et al., 1999, Saeboe-Larssen and Lambertsson, 1996)

RPS13

(Recasens-Alvarez et al., 2021, Pinnola et al., 2007)

Rp S13

(Alonso and Santaren, 2006)

Rp17

Rps 13

(Hsiao et al., 2014)

Rps13

(Schleich et al., 2014)

S13

(Al-Jubran et al., 2013, Molnar et al., 2003)

S15

(Anger et al., 2013)

l(2)k09614

(BDGP Project Members, 1994-1999)

Name Synonyms

Minute(2)32A

(Saeboe-Larsson, 1995.9.26, Saeboe-Larsson, 1995.9.26)

Ribosomal protein S13

(Hsiao et al., 2014, Jaspers et al., 2012, Cammarato et al., 2011)

ribosomal protein S17

(Ashburner, 1992.12.9)

Secondary FlyBase IDs

FBgn0021990

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (100)

The following external sites may use different assemblies or annotations than FlyBase.

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