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General Information
Symbol
Dmel\Dsor1
Species
D. melanogaster
Name
Downstream of raf1
Annotation Symbol
CG15793
Feature Type
FlyBase ID
FBgn0010269
Gene Model Status
Stock Availability
Enzyme Name (EC)
Mitogen-activated protein kinase kinase (2.7.12.2)
Gene Summary
Downstream of raf1 (Dsor1) encodes a serine/threonine kinase that phosphorylates MAP kinase. It is activated by the product of Raf and acts downstream of receptor tyrosine kinases such as those encoded by tor, Egfr, and sev. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
Also Known As

MEK, Dsor, MAPKK, D-mek, DRODSOR1

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
1-27
RefSeq locus
NC_004354 REGION:9247342..9250037
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (26 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:P11346,UniProtKB:Q24171
(assigned by UniProt )
inferred from mutant phenotype
inferred from direct assay
inferred from genetic interaction with SGD:S000004354
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000684494
(assigned by GO_Central )
Biological Process (21 terms)
Terms Based on Experimental Evidence (20 terms)
CV Term
Evidence
References
inferred from genetic interaction with FLYBASE:sl; FB:FBgn0003416
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:fs(1)K10; FB:FBgn0000810
inferred from genetic interaction with FLYBASE:Egfr; FB:FBgn0003731
inferred from genetic interaction with FLYBASE:fs(1)K10; FB:FBgn0000810
inferred from mutant phenotype
inferred from high throughput genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256
inferred from genetic interaction with FLYBASE:hop; FB:FBgn0004864
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with UniProtKB:P51023
(assigned by UniProt )
inferred from direct assay
inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
inferred from genetic interaction with FLYBASE:sev; FB:FBgn0003366
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:tor; FB:FBgn0003733
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in MAPK cascade
inferred from biological aspect of ancestor with PANTHER:PTN000684494
(assigned by GO_Central )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
colocalizes_with nuclear envelope
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. (Q24324)
Catalytic Activity (EC)
Experimental Evidence
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L- seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein] (2.7.12.2)
Predictions / Assertions
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L- seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein] (2.7.12.2)
Summaries
Gene Snapshot
Downstream of raf1 (Dsor1) encodes a serine/threonine kinase that phosphorylates MAP kinase. It is activated by the product of Raf and acts downstream of receptor tyrosine kinases such as those encoded by tor, Egfr, and sev. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
STE7 MAP KINASE KINASES -
STE7 MAP kinase kinases (MAPKKs) belong to the STE7 subfamily and are serine/threonine kinases that phosphorylate MAP kinases in MAPK signalling cascades. MAPKKs are activated by MAP kinase kinase kinases (MAPKKKs). (Adapted from FBrf0225276.)
Pathway (FlyBase)
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
Torso Signaling Pathway Core Components -
The formation of Drosophila embryonic termini is controlled by the localized activation of Torso (tor) receptor tyrosine kinase. The Torso signaling pathway acts via the canonical Ras/Raf/MAP kinase cascade. (Adapted from FBrf0157176.)
Sevenless Signaling Pathway Core Components -
The specification of the R7 photoreceptor cell in each ommatidium of the developing Drosophila eye is dependent on activation of Sevenless receptor tyrosine kinase, which acts via the canonical Ras/Raf/MAP kinase cascade to promote the expression of lz and pros. sev, expressed in presumptive R7 cells, is activated by binding to Bride of Sevenless (boss), a seven-transmembrane protein expressed in R8 cells. (Adapted from FBrf0127283 and FBrf0221727).
EGFR Signaling Pathway Core Components -
The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
FGFR Signaling Pathway Core Components -
Fibroblast Growth Factor Receptor (FGFR) signaling pathway is initiated by the binding of secreted FGFs - bnl or ths/pyr to receptor tyrosine kinases btl or htl, respectively, to initiate signaling primarily via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0221038).
Pvr Signaling Pathway Core Components -
PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).
Protein Function (UniProtKB)
Required downstream of Raf in the sevenless (sev), torso (tor), and Drosophila EGF receptor homolog (DER) signal transduction pathways. Involved in both positive regulation (at the posterior terminus) and negative regulation (at the anterior domain) of tll, as in other terminal class gene products, maybe via the ERK-A kinase.
(UniProt, Q24324)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Dsor1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071313
1799
396
FBtr0340120
1503
393
Additional Transcript Data and Comments
Reported size (kB)

2.0 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071248
43.9
396
6.25
FBpp0309113
43.5
393
6.15
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

393 (aa); 44 (kD)

Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Raf and ksr; Dsor1 binding to ksr probably promotes ksr and Raf dimerization and ksr-mediated Raf transactivation.

(UniProt, Q24324)
Post Translational Modification

Phosphorylation on Ser/Thr by MAP kinase kinase kinases regulates positively the kinase activity.

(UniProt, Q24324)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dsor1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Dsor1 transcripts are expressed throughout development and are particularly abundant in early embryos.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
colocalizes_with nuclear envelope
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Dsor1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 28 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 28 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Dsor1
Transgenic constructs containing regulatory region of Dsor1
Aberrations (Deficiencies and Duplications) ( 5 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
14 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
1  
1 of 15
No
Yes
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
12 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (9)
13 of 13
Yes
Yes
12 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
13 of 12
Yes
Yes
2 of 12
No
Yes
2 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (9)
13 of 15
Yes
Yes
10 of 15
No
Yes
10 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (9)
15 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (10)
9 of 9
Yes
Yes
9 of 9
Yes
Yes
8 of 9
No
Yes
4 of 9
No
Yes
4 of 9
No
Yes
4 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
8 of 15
Yes
Yes
7 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (3)
11 of 12
Yes
Yes
5 of 12
No
Yes
3 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909GK )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505GF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05GY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08J3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0DEC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (15)
4 of 10
4 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 5 )
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 6 )
Allele
Disease
Interaction
References
ameliorates  Huntington's disease
modeled by @Huntington's??not a valid allele symbol??@, @disease??not a valid allele symbol??@
ameliorates  Huntington's disease
modeled by @Huntington's??not a valid allele symbol??@, @disease??not a valid allele symbol??@
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Interacts with Raf and ksr; Dsor1 binding to ksr probably promotes ksr and Raf dimerization and ksr-mediated Raf transactivation.
(UniProt, Q24324 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
Torso Signaling Pathway Core Components -
The formation of Drosophila embryonic termini is controlled by the localized activation of Torso (tor) receptor tyrosine kinase. The Torso signaling pathway acts via the canonical Ras/Raf/MAP kinase cascade. (Adapted from FBrf0157176.)
Sevenless Signaling Pathway Core Components -
The specification of the R7 photoreceptor cell in each ommatidium of the developing Drosophila eye is dependent on activation of Sevenless receptor tyrosine kinase, which acts via the canonical Ras/Raf/MAP kinase cascade to promote the expression of lz and pros. sev, expressed in presumptive R7 cells, is activated by binding to Bride of Sevenless (boss), a seven-transmembrane protein expressed in R8 cells. (Adapted from FBrf0127283 and FBrf0221727).
EGFR Signaling Pathway Core Components -
The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
FGFR Signaling Pathway Core Components -
Fibroblast Growth Factor Receptor (FGFR) signaling pathway is initiated by the binding of secreted FGFs - bnl or ths/pyr to receptor tyrosine kinases btl or htl, respectively, to initiate signaling primarily via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0221038).
Pvr Signaling Pathway Core Components -
PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).
Metabolic Pathways
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-27
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
8D2-8D3
Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912EP912
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
8D-8D
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (22)
Genomic Clones (7)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (21)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of
Additional comments

Wild-type Dsor1 does not complement the mating defect of S.cerevisiae Scer\ste7 mutants. A mutated form of Dsor1 (carrying the lesion found in Dsor1Su1) does complement the mating defect of S.cerevisiae Scer\ste7 mutants. Wild-type Dsor1 does not complement the temperature-sensitive cell-lysis defect of S.cerevisiae Scer\MKK1 Scer\MKK2 double mutants. A mutated form of Dsor1 (carrying the lesion found in Dsor1Su1) does complement the temperature-sensitive cell-lysis defect of S.cerevisiae Scer\MKK1 Scer\MKK2 double mutants. Wild-type Dsor1 or a mutated form of Dsor1 (carrying the lesion found in Dsor1Su1) does not complement the sensitivity to osmotic pressure of S.cerevisiae Scer\pbs2 mutants.

Other Comments

dsRNA made from templates generated with primers directed against this gene.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

The MAPK cascade is required for Ras85D mitogenic response, loss of function mutations in phl, Dsor1, rl and ksr dominantly suppress hyperplastic growth, as do mutations in the Ras85D effector loop that disrupt the Ras85D-phl interaction.

Downstream signal transducer of torso, sevenless and DER receptors.

Studies of interaction between argos and members of the Ras/MAPK pathway demonstrate the argos gene product is a negative regulator of signal transduction that acts upstream of the Ras/MAPK cascade.

Expression of Dsor1 in lin-3, let-23 or lin-45 C.elegans mutants permits P5.p, P6.p and P7.p segments to adopt the vulval fates in each of the vulvaless backgrounds.

Additional alleles of Dsor1 have been isolated in screen for lethal mutations that fail to complement Df(1)10-70d.

A screen to identify mutations affecting the Ras85D signalling pathway identified alleles of phl, Dsor1, rl, aop, βggt-I, mts, ksr and phyl.

Down-regulation of bcd activity depends on the function of Dsor1. Dsor1 acts downstream of phl in the tor pathway and encodes a MAP-kinase kinase (MAPKK). Several clustered consensus sites for MAP kinase phosphorylation can be found in the bcd coding sequence.

Dsor1 acts downstream of phl in the DER pathway.

Dsor1 and phl both play a role in the determination of the rate of proliferation. Maternally derived Dsor1 is required for development of the embryonic termini. Dsor1 is involved in both the positive and negative regulation of tll.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 125 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
modMine - A data warehouse for the modENCODE project
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
BioGRID - A database of protein and genetic interactions.
Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (45)
Reported As
Symbol Synonym
D-MEK/Dsor
Dsor1
(DeAngelis et al., 2021, Fabian et al., 2021, Harnish et al., 2021, Khan et al., 2021, Lebo et al., 2021, Marmion et al., 2021, Nguyen et al., 2021, Yu et al., 2021, Zheng et al., 2021, Alameh et al., 2020, Graves et al., 2020, Hayashi and Ogura, 2020, Kierdorf et al., 2020, Kurihara et al., 2020, Wang et al., 2020, Chai et al., 2019, Fenckova et al., 2019, Das et al., 2018, Du et al., 2018, Gene Disruption Project members, 2018-, Inoue et al., 2018, Lee et al., 2018, Levis, 2018.8.30, Ashton-Beaucage and Therrien, 2017, Goyal et al., 2017, Houtz et al., 2017, Jindal et al., 2017, Lusk et al., 2017, Song et al., 2017, Transgenic RNAi Project members, 2017-, Ashton-Beaucage et al., 2016, Malartre, 2016, Hall and Verheyen, 2015, Zang et al., 2015, Chuang et al., 2014, Gonzalez et al., 2014, Hasygar and Hietakangas, 2014, Kim et al., 2014, Sopko et al., 2014, Bergwitz et al., 2013, Carter, 2013, Chen et al., 2013, Cui et al., 2013, Mbodj et al., 2013, Park et al., 2013, Schertel et al., 2013, Walker et al., 2013, Yamamoto et al., 2013-, Yin et al., 2013, Gomez et al., 2012, Legent et al., 2012, Miller et al., 2012, Friedman et al., 2011, Friedman et al., 2011, Friedman et al., 2011, Maher et al., 2011, Ragab et al., 2011, Zeng et al., 2010, Hill-Burns and Clark, 2009, Sims et al., 2009, Christensen et al., 2008.6.11, Christensen et al., 2008.6.11, Christensen et al., 2008.12.28, Mouchel-Vielh et al., 2008, Xia et al., 2008, Zhou et al., 2008, Nishimura et al., 2007, Nishimura et al., 2007, Guichard et al., 2006, Mogila et al., 2006, Oishi et al., 2006, Walker et al., 2006, Michellod et al., 2003, Dawes-Hoang and Wieschaus, 2001, Lim et al., 1997)
Su(Raf)34B
Name Synonyms
Downstream of Raf1
Drosophila MEK
MAP Kinase Kinase
MAPK kinase
Map kinase kinase
downstream suppressor of Raf 1
mitogen-activated protein kinase kinase
Secondary FlyBase IDs
    Datasets (1)
    Study focus (1)
    Experimental Role
    Project
    Project Type
    Title
    • bait_protein
    Interaction map generated by purification of receptor tyrosine kinase pathway factors, with identification of copurifying proteins by mass spectrometry.
    References (313)