l(2)37Cf, lincRNA.S1565, l(2)E60
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Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.48
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Multiple (sequential) stage-specific extensions of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.
Gene model reviewed during 5.55
4.933 (longest cDNA)
1037 (aa)
Interacts with nos and pum (PubMed:11274060). Acts via the formation of a quaternary complex composed of pum, nos, brat and the 3'-UTR mRNA of hb (PubMed:11274060). Not recruited by nos and pum to cyclin B 3'-UTR mRNA (PubMed:11274060). Might interact with mira; the interaction seems to be important for brat localization during mitosis (PubMed:16564014).
The NHL repeats form six-bladed beta-propeller that mediate the interaction with the pumilio repeats of pum, and are essential for translational effector function.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\brat using the Feature Mapper tool.
Comment: expression starts late in embryonic stage 12
Comment: expression starts late in embryonicstage 12
Comment: expression starts late in embryonic stage 12
Comment: maternally deposited
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as ventral nerve cord anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: extended 3' UTR isoform
brat transcripts are detected early and late in embryogenesis but transcripts containing the 3' UTR extension (~8.5 kb) are only detected at later stages and appear after the maternal-to-zygotic transition. Sequential, phased, 3' UTR lengthening is seen (3' UTR lengthening ocurring in multiple steps during later stages of embryogenesis). brat extended 3' UTR transcripts are expressed specifically in the nervous system.
brat expression is enriched in border follicle cells relative to other cells in the egg chamber.
brat is expressed in the developing brain, the ventral CNS, and the PNS starting at stage 11. Expression is detected in the ganglion mother cells starting at stage 13. In stage 16 embryos, 4 clusters of peripheral neurons express brat. The lacZ reporter construct Ecol\lacZbrat-k06028 shows additional staining in cardioblasts from stage 11 to 17, which was not detected by brat in situ hybridization. See Ecol\lacZbrat-k06028RA for reporter construct expression, including expression in larval stages.
GBrowse - Visual display of RNA-Seq signals
View Dmel\brat in GBrowse 22-54
2-54
2-53.6
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
Source for merge of: brat anon-37CDa
Source for merge of brat anon-37CDa was sequence comparison ( date:991108 ).
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
brat appears to be involved in negatively regulating the level of cellular rRNA.
brat is a translational repressor.
Located between coordinates +45.7 and +50.0.
Mutants display a brain neoplastic phenotype.
Mutations at the brat locus cause defects in midoogenesis.
Lethality occurs as pharate adults. Third-instar larvae of 13 of 14 alleles examined display enlarged brains -- up to eight times normal volume; greatest in presumptive optic center (Hankins and Wright). Brain fragments transplanted into normal adults show uncontrolled proliferation and metastatic invasion of other tissues (Gateff).