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Gene Dmel\brat

General Information
Symbol	Dmel\brat	Species	D. melanogaster
Name	brain tumor	Annotation symbol	CG10719
Feature type	protein_coding_gene	FlyBase ID	FBgn0010300
Gene Model Status	Current	Stock availability	46 publicly available
Also Known As	l(2)37Cf
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	37C1-37C6	Sequence location	2L:19,133,816..19,179,758 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene brain tumor is referred to in FlyBase by the symbol Dmel\brat (CG10719, FBgn0010300). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; translation repressor activity. There is experimental evidence for 17 unique biological process terms, many of which group under: biological regulation; system development; cellular component organization or biogenesis; regulation of neurogenesis; cell fate commitment; single-organism developmental process; neuron differentiation; cell proliferation; regulation of developmental process; cellular process. 109 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; organ system; anatomical structure; adult; organ system subdivision; imaginal precursor; external compound sense organ; portion of tissue; adult segment; ovariole; embryonic/larval imaginal precursor; thoracic segment; abdominal segment; sense organ; non-connected developing system. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: NHL repeat; NHL repeat, subgroup; Six-bladed beta-propeller, TolB-like; Zinc finger, B-box. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1639217_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of brat. Gene sequence location is 2L:19133816..19179758.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(2)37Cf Lethality occurs as pharate adults. Third-instar larvae of 13 of 14 alleles examined display enlarged brains-up to eight times normal volume; greatest in presumptive optic center (Hankins and Wright). Brain fragments transplanted into normal adults show uncontrolled proliferation and metastatic invasion of other tissues (Gateff). Escapers [l(2)37Cf1/l(2)37Cfts3] lethargic and sterile in both sexes [Wright, 1987, Results and Problems in Cell Differentiation (W. Hennig, ed.). Springer-Verlag, Berlin, Heidelberg, pp. 95-120]. Homozygous females [l(2)37Cffs2] retain eggs in ovaries (Schupbach).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 37C1-37C6   Limits computationally determined from genome sequence between P{lacW}Ddck02104 and P{lacW}bratk06028&P{lacW}l(2)k09613k09613
Experimentally Determined Cytological Location
Cytogenetic map Notes References 37C1-37D1   (determined by in situ hybridisation)   (Lukacsovich et al., 2001) 37C1-37D1     (Lukacsovich, 1999.1.13) 37C6-37C7   (determined by in situ hybridisation)   (Spradling et al., 1999) 37C5-37C6   37C5--7 37C6--7   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\brat for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated Multiple (sequential) stage-specific extensions of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated. Gene model reviewed during 5.45 Gene model reviewed during 5.48 Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) brat-RA FBtr0081158  NM_057597   4544   1037 brat-RB FBtr0081159  NM_134302   4963   1037 brat-RC FBtr0081160  NM_206004   12088   1037 brat-RE FBtr0336627  NM_001201913   6258   1061
Additional Transcript Data & Comments
Reported size (kB)	4.933 (longest cDNA) (Arama et al., 2000)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein brat-PA   FBpp0080702   110.4   1037   7.21     NP_476945   AAN11027 brat-PB   FBpp0080703   110.4   1037   7.21     NP_599129   AAF53771 brat-PC   FBpp0089239   110.4   1037   7.21     NP_995726   AAS64719 brat-PE   FBpp0307610   113.2   1061   7.30     NP_001188842   ADV37091
Additional Polypeptide Data & Comments
Reported size (kDa)	1037 (aa) (Arama et al., 2000)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, B-box (IPR000315) NHL repeat, subgroup (IPR013017) Six-bladed beta-propeller, TolB-like (IPR011042) NHL repeat (IPR001258)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA438467   AA440727   AA441364   AA540996   AA696127   AA817353   AA949539   AA979307   AA979648   AE014134 AAF53771   AE014134 AAN11027   AE014134 AAS64719   AB022432 BAA82071   AC005440   AC007176   AE014134 ADV37091   AF119332 AAF03086   AF195870 AAF13926   AF195871 AAF13927   AF195872 AAF13928   AF195873 AAF13929   AI256912   AI256913   AI260331   AI295420   AI402659   AI455286   AI455609   AI455727   AI515283   AI519482   AI531941   AI533153   AI533702   AI534366   AI534424   AI542996   AI543310   AI543467   AQ025779   AQ026503   AW942405   AW944024   AY075583   AY122207 AAM52719   AY129441 AAM76183   BG636946   BG638240   BH759542   BI161848   BI171476   BI228795   BI240200   BI240613   BI361958   BI365232   BI372565   BI578852   BI591185   BI605470   BI613510   BI616159   BI635850   BI636191   BI637781   BI639409   BI639644   BI640462   BP540849   BT125989   CA806845   CA807349   CK660107   CK661735   CK662305   CL868595   CO152684   CO152698   CO153025   CO153042   CO153262   CO153286   CO153298   CO153315   CO153419   CO153541   CO153633   CO153854   CO154086   CO154502   CO154692   CO155059   CO155387   CO155460   CO155620   CO155627   CO155670   CO155860   CO155913   CO155914   CO155941   CO156124   CO156154   CO156235   CO156421   CO156422   CO156423   CO156614   CO156842   CO156947   CO157048   CO176822   CO176926   CO177313   CO178445   CO185712   CO187366   CO187787   CO190952   CO193183   CO262079   CO263672   CO264369   CO264614   CO266759   CO267377   CO268097   CO274329   CO274693   CO276390   CO277041   CO279295   CO279399   CO279442   CO280817   CO281048   CO282208   CO283075   CO284158   CO284377   CO284951   CO285207   CO285409   CO285988   CO287107   CO287630   CO288128   CO289837   CO291953   CO292550   CO293317   CO293753   CO297313   CO299369   CO300398   CO301243   CO301828   CO304873   CO304966   CO306411   CO308764   CO311678   CO312483   CO313379   CO314044   CO314563   CO314889   CO315964   CO316797   CO316897   CO317057   CO317123   CO317753   CO318741   CO319953   CO321214   CO321248   CO323932   CO329052   CO329612   CO332462   CO333617   CO333677   CO333968   CO335324   CO335389   CO335577   CO335584   CO335614   CO335715   CO336086   CO336386   CO336402   CO336514   CO336554   CO336851   CO336952   CO337093   CO337486   CO337487   CO337993   CO338347   CO338348   CO338485   CO338742   CO338775   CO338819   CO338834   CO340441   CO341594   CO343806   CZ468130   CZ468600   CZ469740   CZ470661   CZ472980   CZ475833   CZ476103   CZ477708   CZ477709   CZ479664   CZ479932   CZ479933   CZ480054   CZ487408   CZ488997   CZ489853   DQ138866 ABA86472   DU708617   DU708633   EC057107   EC057122   EC057672   EC057922   EC058333   EC058643   EC061280   EC061615   EC061923   EC064885   EC065773   EC067362   EC067575   EC067956   EC068840   EC070066   EC073302   EC073527   EC073821   EC076733   EC077189   EC084457   EC086680   EC088660   EC089131   EC089469   EC089934   EC090642   EC090780   EC198297   EC198495   EC198802   EC199704   EC200241   EC200408   EC202796   EC202925   EC204390   EC205015   EC205744   EC208274   EC208637   EC213227   EC218392   EC218393   EC219663   EC221242   EC222989   EC225273   EC225907   EC227892   EC230753   EC231561   EC232592   EC232921   EC234097   EC235505   EC236032   EC237167   EC240848   EC241943   EC242475   EC242491   EC243990   EC244023   EC245816   EC245994   EC247034   EC249901   EC250443   EC250448   EC250919   EC252190   EC254762   EC257413   EC263491   EC265199   EV575227   EV593651   FE863524   FE863525   FE863595   FE863653   FE863654   FE863679   FE863680   FE863728   FE863729   FE863784   FE863785   FE863839   FE863840   FE863908   FE863909   FE863910   FE863986   GH744766   GH747344   GH764866   GH766677   GH769842   GO254531
UniProtKB/Swiss-Prot	Q8MQJ9
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi mesoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi organism | ubiquitous   (Fisher et al., 2012) ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage organism | ubiquitous   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 17     (Arama et al., 2000) embryonic stage 12 -- 17   embryonic ventral nervous system   (Arama et al., 2000) Comment:expression starts late in embryonic stage 12 presumptive embryonic/larval peripheral nervous system   (Arama et al., 2000) Comment:expression starts late in embryonicstage 12 embryonic brain   (Arama et al., 2000) Comment:expression starts late in embryonic stage 12 embryonic stage 13 -- 16   embryonic ganglion mother cell   (Arama et al., 2000) embryonic brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 16   peripheral nervous system precursor cluster   (Arama et al., 2000) antenno-maxillary complex   (Arama et al., 2000)
Additional Descriptive Data
	brat is expressed in the developing brain, the ventral CNS, and the PNS starting at stage 11. Expression is detected in the ganglion mother cells starting at stage 13. In stage 16 embryos, 4 clusters of peripheral neurons express brat. The Ecol\lacZ reporter construct Ecol\lacZbrat-k06028 shows additional staining in cardioblasts from stage 11 to 17, which was not detected by brat in situ hybridization. See Ecol\lacZbrat-k06028RA for reporter construct expression, including expression in larval stages. (Arama et al., 2000)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with apical cortex inferred from direct assay (Betschinger et al., 2006) colocalizes_with basal cortex inferred from direct assay (Betschinger et al., 2006) cytoplasm inferred from direct assay (Betschinger et al., 2006) inferred from direct assay (assigned by UniProtKB) (Sonoda and Wharton, 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary  (667.9) Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary  667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    292.15 Larval Midgut    21.9 Larval Hindgut    28.6 Larval Malpighian Tubules    24.9 Larval Fat Body    73.6 Larval Salivary Gland    15.4 Larval Trachea    16.875 Larval Carcass    46.025 Adult Head    7 Adult Eye    6.125 Adult Brain    23.3 Adult Thoracic-Abdominal Ganglion    14.5 Adult Crop    14.3 Adult Midgut    12.9 Adult Hindgut    7 Adult Malpighian Tubules    15.1 Adult Fat Body    24.7 Adult Salivary Gland    13.3 Adult Heart    9.525 Adult VirginFemale Spermatheca    20.9 Adult InseminatedFemale Spermatheca    22.9 Adult Ovary    667.9 Adult Testis    8.1 Adult Male Accessory Gland    37.6 Adult Carcass    16.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    7   NA  Eye    6.125   NA  Brain    23.3   292.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    14.5   NA  Crop    14.3   21.9  Midgut    12.9   28.6  Hindgut    7   24.9  Malpighian Tubules    no informative data   73.6  Fat Body    24.7   15.4  Salivary Gland    no informative data   NA  Heart    9.525   16.875  Trachea    NA   NA  VirginFemale Spermatheca    20.9   NA  InseminatedFemale Spermatheca    22.9   NA  Ovary    667.9   NA  Testis    8.1   NA  Male Accessory Gland    37.6   46.025  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    166 central nervous system, pupae P8    64 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    36 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female    46 ovary, mated 4-day female    37 testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (33) central nervous system, larvae L3  (166) central nervous system, pupae P8  (64) head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae  (44) digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering  (36) carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female  (46) ovary, mated 4-day female  (37) testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3  (166) central nervous system, pupae P8    64 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    36 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female    46 ovary, mated 4-day female    37 testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    166 central nervous system, pupae P8    64 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    36 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female    46 ovary, mated 4-day female    37 testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    166 central nervous system, pupae P8    64 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    36 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female    46 ovary, mated 4-day female    37 testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (33) central nervous system, larvae L3  (166) central nervous system, pupae P8  (64) head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae  (44) digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering  (36) carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female  (46) ovary, mated 4-day female  (37) testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3  (166) central nervous system, pupae P8    64 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    36 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female    46 ovary, mated 4-day female    37 testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    166 central nervous system, pupae P8    64 head, virgin 1-day female    4 head, virgin 4-day female    3 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    5 head, mated 4-day male    7 head, mated 20-day male    6 salivary gland, larvae L3 wandering    7 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    2 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    36 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    2 ovary, virgin 4-day female    46 ovary, mated 4-day female    37 testis, mated 4-day male    2 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    37 embryo 04-06hr    56 embryo 06-08hr    59 embryo 08-10hr    54 embryo 10-12hr    46 embryo 12-14hr    39 embryo 14-16hr    31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    33 white prepupae new    34 white prepupae 12hr    50 white prepupae 24hr    47 pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day    35 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (63) embryo 02-04hr  (37) embryo 04-06hr  (56) embryo 06-08hr  (59) embryo 08-10hr  (54) embryo 10-12hr  (46) embryo 12-14hr  (39) embryo 14-16hr  (31) embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9  (33) white prepupae new  (34) white prepupae 12hr  (50) white prepupae 24hr  (47) pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day  (35) adult female 30day  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    37 embryo 04-06hr    56 embryo 06-08hr    59 embryo 08-10hr    54 embryo 10-12hr    46 embryo 12-14hr    39 embryo 14-16hr    31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    33 white prepupae new    34 white prepupae 12hr    50 white prepupae 24hr    47 pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day    35 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    37 embryo 04-06hr    56 embryo 06-08hr    59 embryo 08-10hr    54 embryo 10-12hr    46 embryo 12-14hr    39 embryo 14-16hr    31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    33 white prepupae new    34 white prepupae 12hr    50 white prepupae 24hr    47 pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day    35 adult female 30day    37 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    37 embryo 04-06hr    56 embryo 06-08hr    59 embryo 08-10hr    54 embryo 10-12hr    46 embryo 12-14hr    39 embryo 14-16hr    31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    33 white prepupae new    34 white prepupae 12hr    50 white prepupae 24hr    47 pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day    35 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (63) embryo 02-04hr  (37) embryo 04-06hr  (56) embryo 06-08hr  (59) embryo 08-10hr  (54) embryo 10-12hr  (46) embryo 12-14hr  (39) embryo 14-16hr  31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9  (33) white prepupae new  (34) white prepupae 12hr  (50) white prepupae 24hr  (47) pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day  (35) adult female 30day  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    37 embryo 04-06hr    56 embryo 06-08hr    59 embryo 08-10hr    54 embryo 10-12hr    46 embryo 12-14hr    39 embryo 14-16hr    31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    33 white prepupae new    34 white prepupae 12hr    50 white prepupae 24hr    47 pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day    35 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    63 embryo 02-04hr    37 embryo 04-06hr    56 embryo 06-08hr    59 embryo 08-10hr    54 embryo 10-12hr    46 embryo 12-14hr    39 embryo 14-16hr    31 embryo 16-18hr    20 embryo 18-20hr    16 embryo 20-22hr    23 embryo 22-24hr    23 larva L1    7 larva L2    3 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    33 white prepupae new    34 white prepupae 12hr    50 white prepupae 24hr    47 pupae 2d postWPP    17 pupae 3d postWPP    4 pupae 4d postWPP    6 adult male 01day    3 adult male 05day    2 adult male 30day    3 adult female 01day    21 adult female 05day    35 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2    29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2  (29) embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3  (27) eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2    29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2    29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2    29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2  29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3  27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2    29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    19 embryonic 1182-4H    15 embryonic GM2    29 embryonic Kc167    21 embryonic S1    14 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    19 wing disc ML-DmD8    20 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    9 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    18 ovary fGS/OSS    21 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult  (30) Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (29) Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult  30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    30 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    14 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD16270 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010300 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10719-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for brat Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal bratGD7073 bratKK113206 lethal | embryonic stage | germline clone | maternal effect | recessive brat2L-150-11 brat2L-192-9 brat2L-257-19 lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive brat2L-150-11 brat2L-192-9 brat2L-257-19 lethal | recessive brat1 brat2 brat3 brat4 brat5 brat6 brat7 brat8 brat10 brat11 brat13 brat14 brat15 brat18 brat19 brat20 brat21 brat22 brat23 brat24 bratfs3 bratk06028 bratk11508 bratk11523 bratk11538 bratLA00213 bratts1 lethal | recessive | heat sensitive bratts1 bratts2 bratts3 partially lethal - majority die | recessive brat1 brat9 brat12 brat14 brat16 brat17 viable bratEY13747 bratGTD1-2.2 bratGTD1-31.1 bratLA00378 bratRab12 viable, with Scer\GAL4pnr-MD237 bratGD7073 Sterility Allele female sterile bratfs3 female sterile, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratR837D.Scer\UAS.P\T bratScer\UAS.P\T.cHa female sterile | recessive bratfs1 bratfs2 bratfs3 fertile bratEY13747 bratLA00378 Other Phenotypes Allele decreased cell number, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratScer\UAS.P\T.cHa decreased cell number | cell non-autonomous, with Scer\GAL4en-e16E bratScer\UAS.cSa decreased cell number | somatic clone, with Scer\GAL4Act5C.PP bratScer\UAS.cFa increased cell number | germline clone brat11 increased cell size, with Scer\GAL4dpp.blk1 bratScer\UAS.cFa increased cell size | germline clone brat11 increased cell size | somatic clone brat11 increased cell size | somatic clone, with Scer\GAL4Act5C.PP bratScer\UAS.cFa mitotic cell cycle defective brat11 mitotic cell cycle defective | somatic clone brat2L-192-9 brat11 neuroanatomy defective brat2L-150-11 brat11 neuroanatomy defective | larval stage bratGD7073 neuroanatomy defective | larval stage (with brat11) bratDG19310 bratk06028 neuroanatomy defective | larval stage (with bratDG19310) brat11 neuroanatomy defective | larval stage (with bratk06028) brat11 neuroanatomy defective | somatic clone brat11 neuroanatomy defective | somatic clone | third instar larval stage bratK1771 tumorigenic brat1 brat2L-150-11 bratk06028 tumorigenic (with brat11) brat14 tumorigenic (with brat14) brat11 brat18 tumorigenic (with brat18) brat14 tumorigenic | somatic clone brat2L-192-9 tumorigenic | third instar larval stage brat11 visible, with Scer\GAL4ey.PH bratScer\UAS.cFa visible | cell non-autonomous, with Scer\GAL4en-e16E bratScer\UAS.cSa wild-type brat+t5.8 wild-type, with Scer\GAL4en-e16E bratfs1.Scer\UAS Phenotype manifest in Allele 1st posterior cell, with Scer\GAL4dpp.blk1 bratScer\UAS.cFa adult brain | somatic clone brat11 adult thorax (with brat1) brat7 adult thorax (with brat7) brat1 axon | somatic clone brat2L-192-9 egg chamber, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratScer\UAS.P\T.cHa bratR837D.Scer\UAS.P\T embryo | germline clone | maternal effect | posterior brat2L-150-11 brat2L-192-9 brat2L-257-19 embryonic/larval brain brat1 brat11 brat18 bratk06028 embryonic/larval brain (with brat14) brat18 embryonic/larval brain (with brat18) brat14 embryonic/larval brain | cell autonomous | somatic clone brat11 embryonic/larval brain | posterior brat18 embryonic/larval neuroblast | larval stage bratGD7073 embryonic abdomen bratfs1 bratk06028 embryonic abdomen (with brat1) bratfs3 embryonic abdomen (with bratfs1) bratfs3 embryonic abdomen (with bratfs3) brat1 bratfs1 bratk06028 embryonic abdomen (with bratk06028) bratfs3 embryonic abdomen (with Df(2L)TE37C-7) bratfs1 bratfs3 embryonic abdominal segment bratk06028 embryonic abdominal segment (with brat1) bratfs3 embryonic abdominal segment (with bratfs1) bratfs3 embryonic abdominal segment (with bratfs3) brat1 bratfs1 bratk06028 embryonic abdominal segment (with bratk06028) bratfs3 embryonic abdominal segment (with Df(2L)TE37C-7) bratfs1 bratfs3 embryonic abdominal segment | maternal effect (with Df(2L)TE37C-7) bratfs1 embryonic epidermis bratk06028 embryonic epidermis (with brat1) bratfs3 embryonic epidermis (with bratfs1) bratfs3 embryonic epidermis (with bratfs3) brat1 bratfs1 bratk06028 embryonic epidermis (with bratk06028) bratfs3 embryonic epidermis (with Df(2L)TE37C-7) bratfs1 bratfs3 eye, with Scer\GAL4ey.PH bratScer\UAS.cFa female germline cell, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratR837D.Scer\UAS.P\T female germline cyst, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratScer\UAS.P\T.cHa female germline stem cell, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratScer\UAS.P\T.cHa female germline stem cell | germline clone | supernumerary brat11 ganglion mother cell brat11 brat2L-150-11 germarium, with Scer\GAL4nos.UTR.T:Hsim\VP16 bratR837D.Scer\UAS.P\T imaginal disc (with brat14) brat18 imaginal disc (with brat18) brat14 mitotic cell cycle | somatic clone, with Scer\GAL4Act5C.PP bratScer\UAS.cFa neuroblast | somatic clone brat2L-150-11 brat2L-192-9 neuroblast | supernumerary brat11 brat2L-150-11 neuron brat2L-150-11 nucleolus | somatic clone brat11 RP2 motor neuron | maternal effect brat2L-150-11 RP2 motor neuron | supernumerary, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 bratScer\UAS.T:Avic\GFP-EGFP type II neuroblast | larval stage | supernumerary bratHM05078 type II neuroblast | larval stage | supernumerary (with brat11) bratk06028 bratDG19310 type II neuroblast | larval stage | supernumerary (with bratDG19310) brat11 type II neuroblast | larval stage | supernumerary (with bratk06028) brat11 wing & trichogen cell bratunspecified wing | anterior compartment | cell non-autonomous, with Scer\GAL4en-e16E bratScer\UAS.cSa
Classical Alleles ( 87 )
For All Classical Alleles Show
Allele of brat Class Mutagen Stocks Known lesion brat1 ethyl methanesulfonate 2 -- bratLA00213 P-element activity 2 -- bratLA00378 P-element activity 2 -- bratts1 ethyl methanesulfonate 2 Yes brat18 ethyl methanesulfonate gamma ray 1 Yes brat5-HA-1269 1 -- brat5-SZ-4002 1 -- bratBG02721 P-element activity 1 -- bratc05100 piggyBac transposase 1 -- bratc06399 piggyBac transposase 1 -- bratCB-0248-3 1 -- bratCB-0304-3 1 -- bratCB-0898-3 1 -- bratCB-5066-3 1 -- bratCB-5855-3 1 -- bratd06071 1 -- bratd07606 P-element activity 1 -- bratd08172 P-element activity 1 -- bratd11404 P-element activity 1 -- brate01842 piggyBac transposase 1 -- brate02105 piggyBac transposase 1 -- brate02225 piggyBac transposase 1 -- bratEY01093 1 -- bratEY02091 1 -- bratEY13747 P-element activity 1 -- bratf04835 piggyBac transposase 1 -- bratf06309 piggyBac transposase 1 -- bratf07082 piggyBac transposase 1 -- bratfs1 ethyl methanesulfonate 1 -- bratfs3 ethyl methanesulfonate 1 Yes bratk06028 P-element activity 1 Yes bratk11508 P-element activity 1 -- bratKG02716 1 -- bratKG04933 P-element activity 1 -- bratKG08647 P-element activity 1 -- bratMI02320 1 -- bratMI02407 1 -- bratRab12 1 Yes brat10 ethyl methanesulfonate formaldehyde 0 -- brat11 ethyl methanesulfonate formaldehyde 0 Yes brat12 ethyl methanesulfonate 0 -- brat13 ethyl methanesulfonate 0 -- brat14 ethyl methanesulfonate 0 Yes brat15 ethyl methanesulfonate 0 -- brat16 ethyl methanesulfonate gamma ray 0 -- brat17 ethyl methanesulfonate gamma ray 0 -- brat19 ethyl methanesulfonate 0 -- brat20 diepoxybutane 0 -- brat21 ethyl methanesulfonate 0 -- brat22 ethyl methanesulfonate gamma ray 0 -- brat23 ethyl methanesulfonate gamma ray 0 -- brat24 ethyl methanesulfonate gamma ray 0 -- brat2 ethyl methanesulfonate 0 -- brat2L-150-11 ethyl methanesulfonate 0 Yes brat2L-192-9 ethyl methanesulfonate 0 Yes brat2L-257-19 ethyl methanesulfonate 0 -- brat3 ethyl methanesulfonate 0 -- brat4 ethyl methanesulfonate 0 -- brat5-HA-2302 0 -- brat5 ethyl methanesulfonate 0 -- brat6 ethyl methanesulfonate 0 -- brat7 ethyl methanesulfonate 0 -- brat8 ethyl methanesulfonate formaldehyde 0 -- brat9 ethyl methanesulfonate formaldehyde 0 -- bratc02693 piggyBac transposase 0 -- bratc03328 piggyBac transposase 0 -- bratCB-0247-3 0 -- bratd02758 P-element activity 0 -- bratDG08705 0 -- bratDG19310 P-element activity 0 -- brate00037 0 -- brate00880 0 -- bratEP2483 P-element activity 0 -- bratEP778 P-element activity 0 -- bratfs2 ethyl methanesulfonate 0 -- bratGS-A2233 Delta2-3 0 -- bratGS11180 0 -- bratGTD1-2.2 P-element activity 0 Yes bratGTD1-31.1 P-element activity 0 Yes bratk11523 P-element activity 0 Yes bratk11538 P-element activity 0 -- bratK1771 ethyl methanesulfonate 0 Yes bratNP4695 0 -- bratts2 ethyl methanesulfonate formaldehyde 0 -- bratts3 ethyl methanesulfonate 0 -- bratunspecified 0 -- bratUY1131 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of brat Class Mutagen Stocks Known lesion bratGD7073 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bratHM05078 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bratHMS01121 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bratKK113206 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes brat+t5.8 in vitro construct - genomic fragment 0 Yes bratfs1.Scer\UAS in vitro construct - amino acid replacement 0 Yes bratG774D.Scer\UAS.P\T in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes bratGMR.PB in vitro construct - regulatory fusion 0 Yes braths.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes bratK882E.nos in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes bratNIG.10719R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes bratnos.PC in vitro construct - regulatory fusion 0 Yes bratR837D.nos in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes bratR837D.Scer\UAS.P\T in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes bratScer\UAS.cFa in vitro construct - regulatory fusion 0 Yes bratScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes bratScer\UAS.P\T.cHa in vitro construct - regulatory fusion 0 Yes bratScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes bratScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes bratΔ63-721.fs1.Scer\UAS in vitro construct - deletion 0 Yes bratΔ63-721.Scer\UAS in vitro construct - deletion 0 Yes bratΔ724-1037.Scer\UAS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)E71 (Wright et al., 1981, Brittnacher and Ganetzky, 1983) Df(2L)hk39 (Brittnacher and Ganetzky, 1983) Df(2L)NST (Pentz et al., 1986, Stathakis et al., 1995, Wright, 1996) Df(2L)Sd57 (Brittnacher and Ganetzky, 1983, Stathakis et al., 1995, Wright, 1996) Df(2L)TE37C-1 (Wright et al., 1981, Gilbert et al., 1984, Wright, 1996) Df(2L)TE37C-7 (Stathakis et al., 1995, Wright, 1996, Cho et al., 2006) Df(2L)TW50 (Spradling et al., 1999, Wright et al., 1976, Schupbach and Wieschaus, 1991, BDGP Project Members, 1994-1999) Df(2L)TW130 (Wright et al., 1976, Wright et al., 1981, Brittnacher and Ganetzky, 1983, Gilbert et al., 1984, Schupbach and Wieschaus, 1991, Stathakis et al., 1995, Wright, 1996) Df(2L)TW158 (Brittnacher and Ganetzky, 1983) Df(2L)VA12 (Wright et al., 1981, Gilbert et al., 1984, Pentz et al., 1986, BDGP Project Members, 1994-1999, Stathakis et al., 1995, Wright, 1996) Df(2L)VA13 (Wright et al., 1981, Gilbert et al., 1984, Wright, 1996) Df(2L)VA14 (Brittnacher and Ganetzky, 1983) Df(2L)VA16 (Brittnacher and Ganetzky, 1983) Df(2L)VA17 (Wright et al., 1981, Gilbert et al., 1984, Pentz et al., 1986, BDGP Project Members, 1994-1999, Stathakis et al., 1995, Wright, 1996) Df(2L)VA19 (Brittnacher and Ganetzky, 1983, BDGP Project Members, 1994-1999, Stathakis et al., 1995, Wright, 1996) Df(2L)VA20 (Brittnacher and Ganetzky, 1983) Df(2L)VA23 (Stathakis et al., 1995)
Not disrupted in	Df(2L)E55 (Brittnacher and Ganetzky, 1983, Stathakis et al., 1995) Df(2L)hk18 (Gilbert et al., 1984, Pentz et al., 1986, Wright, 1996) Df(2L)hk-UC1 (Stathakis et al., 1995, Wright, 1996) Df(2L)hk-UC2 (Stathakis et al., 1995, Wright, 1996) Df(2L)M36F-S5 (BDGP Project Members, 1994-1999) Df(2L)OD15 (Pentz et al., 1986, BDGP Project Members, 1994-1999, Stathakis et al., 1995, Wright, 1996) Df(2L)pr26 (Brittnacher and Ganetzky, 1983) Df(2L)Sd2 (Brittnacher and Ganetzky, 1983) Df(2L)Sd14 (Brittnacher and Ganetzky, 1983) Df(2L)Sd37 (Brittnacher and Ganetzky, 1983, Stathakis et al., 1995, Wright, 1996) Df(2L)Sd77 (Brittnacher and Ganetzky, 1983, BDGP Project Members, 1994-1999, Stathakis et al., 1995) Df(2L)TW2 (Brittnacher and Ganetzky, 1983) Df(2L)TW9 (BDGP Project Members, 1994-1999) Df(2L)TW137 (BDGP Project Members, 1994-1999, Stathakis et al., 1995) Df(2L)TW203 (Pentz et al., 1986, Stathakis et al., 1995, Wright, 1996) Df(2L)VA6 (Brittnacher and Ganetzky, 1983) Df(2L)VA8 (Brittnacher and Ganetzky, 1983) Df(2L)VA18 (Gilbert et al., 1984, Pentz et al., 1986, BDGP Project Members, 1994-1999, Stathakis et al., 1995, Wright, 1996) Df(2L)VA21 (Stathakis et al., 1995, Wright, 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7073} NA P{KK113206} NA P{NIG.10719R} NA P{TRiP.HM05078} NA P{TRiP.HMS01121} NA P{UAS-brat.F} NA P{UAS-brat.fs1.Δ63-721} NA P{UAS-brat.fs1} NA P{UAS-brat.GFP} NA P{UAS-brat.myc} NA P{UAS-brat.S} NA P{UAS-brat.Δ63-721} NA P{UAS-brat.Δ724-1037} NA P{UASp-brat.G774D} NA P{UASp-brat.H} NA P{UASp-brat.R837D} NA heat-shock construct P{hs-brat.TAP} NA characterization construct P{GMR-brat.B} NA P{nos-brat.C} NA P{nos-brat.K882E} NA P{nos-brat.R837D} NA P{pC15} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}bratEP778 NA P{EP}bratEP2483 NA P{EPgy2}bratEY01093 NA P{EPgy2}bratEY02091 NA P{EPgy2}bratEY13747 NA P{GSV1}bratGS-A2233 NA P{GSV6}bratGS11180 NA P{Mae-UAS.6.11}bratLA00213 NA P{Mae-UAS.6.11}bratLA00378 NA P{Mae-UAS.6.11}bratUY1131 NA P{XP}bratd02758 NA P{XP}bratd06071 NA P{XP}bratd07441 NA P{XP}bratd07606 NA P{XP}bratd08172 NA P{XP}bratd11404 NA miscellaneous insertions Mi{MIC}bratMI02320 NA Mi{MIC}bratMI02407 NA P{GT1}bratBG02721 NA P{GTD-b}bratGTD1-2.2 NA P{GTD-b}bratGTD1-31.1 NA P{RS3}bratCB-0247-3 NA P{RS3}bratCB-0248-3 NA P{RS3}bratCB-0304-3 NA P{RS3}bratCB-0898-3 NA P{RS3}bratCB-5066-3 NA P{RS3}bratCB-5855-3 NA P{RS5}brat5-HA-1269 NA P{RS5}brat5-HA-2302 NA P{RS5}brat5-SZ-4002 NA P{SUPor-P}bratKG02716 NA P{SUPor-P}bratKG04933 NA P{SUPor-P}bratKG08647 NA P{UASp-YFP.Rab18.A64L}bratRab12 NA P{wHy}bratDG08705 NA P{wHy}bratDG19310 NA PBac{PB}bratc02693 NA PBac{PB}bratc03328 NA PBac{PB}bratc05100 NA PBac{PB}bratc06399 NA PBac{RB}brate00037 NA PBac{RB}brate00880 NA PBac{RB}brate01842 NA PBac{RB}brate02105 NA PBac{RB}brate02225 NA PBac{WH}bratf04835 NA PBac{WH}bratf06309 NA PBac{WH}bratf07082 NA insertion of enhancer trap P{lacW}bratk06028 No P{lacW}bratk11508 No P{lacW}bratk11523 No P{lacW}bratk11538 No P{lacW}l(2)SH0906SH0906 No insertion of enhancer trap binary system P{GawB}bratNP4695 No
Gene Ontology: Function, Process & Cellular Component ( 25 unique terms )
Terms Based on Experimental Evidence ( 22 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with 4EHP (Cho et al., 2006) inferred from physical interaction with nos (assigned by UniProtKB) (Sonoda and Wharton, 2001) translation repressor activity inferred from mutant phenotype (assigned by UniProtKB) (Sonoda and Wharton, 2001)
Biological Process
CV term Evidence References
asymmetric cell division inferred from mutant phenotype (Betschinger et al., 2006) asymmetric neuroblast division resulting in ganglion mother cell formation inferred from mutant phenotype (Lee et al., 2006) axon guidance inferred from mutant phenotype (Berger et al., 2008) brain development inferred from mutant phenotype (Bello et al., 2006) ganglion mother cell fate determination inferred from mutant phenotype (Betschinger et al., 2006) negative regulation of cell proliferation inferred from mutant phenotype (Bello et al., 2006) negative regulation of neuroblast proliferation inferred from mutant phenotype (Betschinger et al., 2006) neuroblast proliferation inferred from mutant phenotype (Caussinus and Gonzalez, 2005) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) neuron differentiation inferred from mutant phenotype (Lee et al., 2006) oogenesis inferred from mutant phenotype (Schupbach and Wieschaus, 1991) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) regulation of neurogenesis inferred from mutant phenotype (Zhu et al., 2011) regulation of translation inferred from mutant phenotype (assigned by UniProtKB) (Sonoda and Wharton, 2001) rRNA metabolic process inferred from mutant phenotype (assigned by UniProtKB) (Frank et al., 2002) rRNA transcription inferred from mutant phenotype (assigned by UniProtKB) (Frank et al., 2002) segmentation inferred from mutant phenotype (Cho et al., 2006)
Cellular Component
CV term Evidence References
colocalizes_with apical cortex inferred from direct assay (Betschinger et al., 2006) colocalizes_with basal cortex inferred from direct assay (Betschinger et al., 2006) cytoplasm inferred from direct assay (Betschinger et al., 2006) inferred from direct assay (assigned by UniProtKB) (Sonoda and Wharton, 2001)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
translation regulator activity traceable author statement (Dean et al., 2002) zinc ion binding inferred from electronic annotation with InterPro:IPR000315 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of cell cycle non-traceable author statement (Lev et al., 2003) regulation of translation traceable author statement (Dean et al., 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions brat allele Gene References bratScer\UAS.cFa BacA\p35 (Frank et al., 2002) unspecified ct (Krupp et al., 2005) Nsf2 (Laviolette et al., 2005) pros (Bello et al., 2006, Betschinger et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61181 DroID - A comprehensive database of gene and protein interactions. • FBgn0010300 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35197
Orthologs
OrthoDB Orthologs (32) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NP7P3 Dipteran inclusive ortholog search EOG6M920P Insect inclusive ortholog search EOG6M384M Arthropod inclusive ortholog search EOG6FQZ6Q Metazoa inclusive ortholog search EOG6QVR50
Orthologs in Drosophila Species (EOG6NP7P3)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\brat     Drosophila simulans   Dsim\GD24189     Drosophila simulans   Dsim\GD24188     Drosophila sechellia   Dsec\GM17330 Y   Drosophila erecta   Dere\GG21163 Y   Drosophila yakuba   Dyak\GE13236 Y   Drosophila ananassae   Dana\GF14706 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10519 Y   Drosophila persimilis   Dper\GL21191 Y   Drosophila willistoni   Dwil\GK23851 Y   Drosophila virilis   Dvir\GJ18246 Y   Drosophila mojavensis   Dmoj\GI13993 Y   Drosophila grimshawi   Dgri\GH13439 Y
Orthologs in non-Drosophila Dipterans (EOG6M920P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004918   Anopheles gambiae Malaria mosquito  Agam\AGAP010054   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017329
Orthologs in non-Dipteran Insects (EOG6M384M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\brat   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004513   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11752   Atta cephalotes Leafcutter ant  Acep\ACEP21071   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13062   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12629   Linepithema humile Argentine ant  Lhum\LH16343   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14771   Solenopsis invicta Red fire ant  Sinv\SINV19300   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007272   Bombyx mori Silkmoth  Bmor\BGIBMGA009425   Pediculus humanus Human body louse  Phum\PHUM061260   Tribolium castaneum Red flour beetle  Tcas\brat
Orthologs in non-Insect Arthropods (EOG6FQZ6Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Sirt6   Ixodes scapularis Deer tick  Isca\ISCW011089
Orthologs in non-Arthropod Metazoa (EOG6QVR50)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ncl-1
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM17330 Drosophila erecta Dere\GG21163 Drosophila yakuba Dyak\GE13236 Drosophila ananassae Dana\GF14706 Drosophila pseudoobscura pseudoobscura Dpse\GA10519 Drosophila persimilis Dper\GL21191 Drosophila willistoni Dwil\GK23851 Drosophila virilis Dvir\GJ18246 Drosophila mojavensis Dmoj\GI13993 Drosophila grimshawi Dgri\GH13439
Stocks & Reagents
Stocks Listed in FlyBase ( 46 )
Harvard	- P{XP}bratd06071 - P{XP}bratd07606 - P{XP}bratd08172 - P{XP}bratd11404 - PBac{PB}bratc05100 - PBac{PB}bratc06399 - PBac{RB}brate01842 - PBac{RB}brate02105 - PBac{RB}brate02225 - PBac{WH}bratf04835 - PBac{WH}bratf06309
VDRC	v105054 P{KK113206}VIE-260B
	More stocks available...
Genomic Clones ( 2 )
	BACR10I09 BACR48O01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 285 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD16270 LP03649 SD08734
Other clones	LD28374
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH21918 GM05115 LD12586 LD15595 LD33693 LD34351 LD39090 LD47040 LP03650 LP04128 LP09043 LP18376 LP21410 LP22401 RE01334 RE13264 RE26439 RE36892 RE37493 RE46392 RE50475 RE59560 RE72783 RH04916 RH42673 RH46033 RH71082 SD03255 SD04880 SD05613 SD07166 SD07237 SD09546 SD10028 SD10233 SD14777 SD17368 SD17795 SD19856 SD21979 SD22280 SD23329
Other clones	12535 13281 14402 20185 20212 20678 29836 29972 30617 35005 36779 40148 41769 44684 47304 52598 54002 55352 56675 58021 60417 71006 72126 73355 78201 83021 102469 106805 108297 108427 117915 118885 124068 124191 124192 155927 162410 162798 172983 175731 176449 176675 191044 192404 193664 194114 194779 214289.28 214505.20 214531.20 281909 282115 569156 571304 CK01.8A.G2 CK01.8C.F12 CK01.23B.D3 CK01.28D.G1 CK01.39D.G4 CK01.55B.H5 CK01.63B.B4 CK01.71C.H1 CK01.82B.G3 CK01.83A.B6 CK01.85D.C7 CK01.93B.H4 CK01.99B.A2 CK01.99C.A6 CK01.106D.H7 CK01.116C.H8 CK01.118D.C2 CK01.126A.D4 Dmel_dig_001819_0607_3664 Dmel_neb_012324_0635_2823 EC35257 EK010957 EK028611 EK033344 EK050229 EK051240 EK051629 EK053136 EK056722 EK061405 EK064926 EK066339 EK067812 EK072207 EK084335 EK089232 EK090141 EK113820 EK115139 EK120716 EK122733 EK133959 EK134233 EK134329 EK138826 EK139734 EK147440 EK149842 EK154934 EK155842 EK159812 EK162211 EK165407 EK165926 EK167708 EK168513 EK168926 EK175158 EK178113 EK180518 EK182253 EK192005 EK198847 EK200427 EK202552 EK204502 EK216640 EK225828 EK228924 EK232456 EK234760 EK237026 EK238156 EK246619 EK246821 EK251001 EK259261 EK263224 EK265903 EK266114 EK266958 EK267155 EK269008 EK273718 EK281546 EK290520 EK290560 EK291939 EK293241 EK303527 EK307450 EK307650 EK308505 EN00202 EN00226 EN01122 EN01143 EN01606 EN01645 EN01660 EN01726 EN01929 EN02319 EN02463 EN02945 EN03448 EN04246 EN04615 EN05308 EN05945 EN06105 EN06434 EN06441 EN06533 EN06856 EN06956 EN06957 EN07055 EN07403 EN07442 EN07625 EN08032 EN08033 EN08034 EN08416 EN09419 EN09630 EN09833 EN10026 EN10236 EN10749 EN10756 EN10830 EN11043 EN11805 EN12430 EN12449 EN12701 EN12748 EN13351 EN13536 EN13822 EN14639 EN14640 EN15734 EN16548 EN16549 EN16922 EN17523 EN17561 EN17661 EN17727 EP10648 EP12749 EP18061 ESG015b.E21_C06 ESG017c.B21.1_C02 FGA008D08 LD20735 LD22611 LD35135 LD35384 RM07452 RM07453 RM07552 RM07652 RM07653 RM07752 RM07753 RM07852 RM07853 RM07952 RM07953 RM07954 RM08052 RM08053 RM08054 RM08152 RM08153 RP01588 RP001009431 RP001029352 RP001052344 RP001053187 RP001054691
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010300 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35197
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: brat anon-37CDa (FlyBase, 1996-)
Additional comments	Source for merge of brat anon-37CDa was sequence comparison (date:991108). (FlyBase, 1996-)
Other Comments
	brat-induced metastases express both neuronal (elav) and glial cell (repo) type markers. (Beaucher et al., 2007) Loss of brat in larvae causes neoplastic brain tumors. Fragments of brat brain tumours transplanted into adult hosts over-proliferate and kill their hosts within 2 weeks. (Beaucher et al., 2007) The brat gene product acts as a posttranscriptional inhibitor of dm in one of the two daughter cells of larval neuroblasts. (Betschinger et al., 2006) brat and 4EHP interact physically and both play a role in hb regulation. (Cho et al., 2006) S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) brat appears to be involved in negatively regulating the level of cellular rRNA. (Frank et al., 2002) brat is a translational repressor. (Sonoda and Wharton, 2001) Located between coordinates +45.7 and +50.0. (Stathakis et al., 1995) Mutants display a brain neoplastic phenotype. (Mechler, 1994) Mutations at the brat locus cause defects in midoogenesis. (Schupbach and Wieschaus, 1991) Lethality occurs as pharate adults. Third-instar larvae of 13 of 14 alleles examined display enlarged brains -- up to eight times normal volume; greatest in presumptive optic center (Hankins and Wright). Brain fragments transplanted into normal adults show uncontrolled proliferation and metastatic invasion of other tissues (Gateff).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057597 NM_134302 NM_206004 NM_001201913 RefSeq (Proteins) • NP_476945 NP_599129 NP_995726 NP_001188842 DDBJ / EMBL / GenBank • AA438467 AA441364 AA696127 AA817353 AA949539 AA979307 AA979648 AAF03086 AAF13926 AAF13927 AAF13928 AAF13929 AAF53771 AAM52719 AAM76183 AAN11027 AAS64719 AB022432 ABA86472 AC005440 AC007176 ADV37091 AF119332 AF195870 AF195871 AF195872 AF195873 AI256912 AI455286 AI455609 AI455727 AI515283 AI519482 AI531941 AI533702 AI543467 AQ025779 AQ026503 AW942405 AW944024 AY122207 AY129441 BAA82071 BH759542 CL868595 CZ468130 CZ468600 CZ469740 CZ470661 CZ472980 CZ475833 CZ476103 CZ477708 CZ477709 CZ479664 CZ479932 CZ479933 CZ480054 CZ487408 CZ488997 CZ489853 DQ138866 DU708617 DU708633 Entrez Gene - A searchable database of RefSeq genes. • 35197 UniProtKB/Swiss-Prot • Q8MQJ9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, B-box (IPR000315) NHL repeat, subgroup (IPR013017) Six-bladed beta-propeller, TolB-like (IPR011042) NHL repeat (IPR001258) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD16270
Linkouts
	BioGRID - A database of protein and genetic interactions • 61181 DroID - A comprehensive database of gene and protein interactions. • FBgn0010300 DRSC - Results from RNAi screens. • FBgn0010300 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010300 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10719-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010300 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35197 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/brat1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35197 modMine - Data generated by the modENCODE project. • FBgn0010300
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	anon-37CDa   brat (Wodarz and Gonzalez, 2006, Laviolette et al., 2005, McCrady and Tolin, 1994, Muraro et al., 2008, Caussinus and Gonzalez, 2005, Tountas and Fortini, 2007, Stroschein-Stevenson et al., 2006, Bowler et al., 2006, Wang et al., 2006, Quinones-Coello, 2007, Lee et al., 2006, Zhang et al., 2007, O'Farrell et al., 2008, FlyBase Curators, 2008, Beaucher et al., 2007, Neumuller et al., 2008, Hilgers et al., 2011, Lee et al., 2006, Tadros et al., 2007, Bowman et al., 2008, Beaucher et al., 2007, Sousa-Nunes et al., 2009, Ayroles et al., 2009, Krejcí et al., 2009, Stone and Ayroles, 2009, Mihailovich et al., 2012, Flaherty et al., 2009, Robine et al., 2009, Hilgers et al., 2012, Berger et al., 2008, Beckstead et al., 2005, Temme et al., 2010, Janic et al., 2010, Harris et al., 2011, Chang et al., 2010, Neumüller et al., 2011, Loop et al., 2004, Berger et al., 2012, Smibert et al., 2012, Abruzzi et al., 2011, Popkova et al., 2012, Basto et al., 2008, Zhu et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Xiao et al., 2012, Miles et al., 2012, Li et al., 2012, Justiniano et al., 2012) Brat (Cho et al., 2006, Betschinger et al., 2006, Yamazaki et al., 2007, Speicher et al., 2007, Neumueller et al., 2007, Wang et al., 2007, Harris and Ashe, 2008, Sousa-Nunes et al., 2008, Chia et al., 2008, Bowman et al., 2006, Slack et al., 2007, Speicher et al., 2008, Brun et al., 2006, Wang et al., 2009, Nie et al., 2009, Hessle et al., 2009) BRAT (Kurusu et al., 2009) Cf (McCrady and Tolin, 1994) CG10719 (Rehwinkel et al., 2006, Quinones-Coello, 2007, Krejcí et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Berger et al., 2008, Ni et al., 2010.12.1) E60   fs(2)ltoPM43   l(2)37Cf (Callahan et al., 1996, Callahan et al., 1995, Mechler, 1990, Gateff and Mechler, 1989, Pentz et al., 1986, Gilbert et al., 1984, Brittnacher and Ganetzky, 1983, Wright et al., 1981, Wright, 1987) l(2)brat   l(2)E60 (Wright et al., 1976, )
Name Synonym	Brain Tumor (Sonoda and Wharton, 2001, Lin, 2008, Speicher et al., 2008) brain tumor (Bello et al., 2006, Tountas and Fortini, 2007, O'Farrell et al., 2008, Beaucher et al., 2007, Kankel et al., 2007, Wang et al., 2006, Mastushita-Sakai et al., 2010, Brun et al., 2006, Stone and Ayroles, 2009, Flaherty et al., 2009, Robine et al., 2009, Beckstead et al., 2005, Temme et al., 2010, Janic et al., 2010, Loop et al., 2004, Basto et al., 2008, Miles et al., 2012, Justiniano et al., 2012) Brain tumor (Cho et al., 2006, Chia et al., 2008, Lee et al., 2006, Beaucher et al., 2007, Krejcí et al., 2009) brain tumour (Southall and Brand, 2009)
Secondary FlyBase IDs
	FBgn0002034 FBgn0004827 FBgn0027355
References ( 163 )
Generate a list of	All references relating to this gene ( 163 )
List References by type	Research paper  ( 87 ) Supplementary material  ( 10 ) Review  ( 19 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 20 ) FlyBase analysis  ( 3 ) Conference report  ( 1 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 4 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 )
Recent research papers ( 16 )
	Adamson and Lajeunesse, 2012, ScientificWorldJournal 2012: 347597 A Study of Epstein-Barr Virus BRLF1 Activity in a Drosophila Model System. [FBrf0218446] Berger et al., 2012, Cell Rep. 2(2): 407--418 FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. [FBrf0219320] Hilgers et al., 2012, Genes Dev. 26(20): 2259--2264 ELAV mediates 3' UTR extension in the Drosophila nervous system. [FBrf0219699] Justiniano et al., 2012, PLoS ONE 7(2): e31417 Loss of the Tumor Suppressor Pten Promotes Proliferation of Drosophila melanogaster Cells In Vitro and Gives Rise to Continuous Cell Lines. [FBrf0217611] Li et al., 2012, Development 139(9): 1547--1556 Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling. [FBrf0218009] Mihailovich et al., 2012, RNA 18(1): 53--64 Widespread generation of alternative UTRs contributes to sex-specific RNA binding by UNR. [FBrf0216923] Miles et al., 2012, Genes Dev. 26(4): 356--368 Pumilio facilitates miRNA regulation of the E2F3 oncogene. [FBrf0217481] Popkova et al., 2012, PLoS Genet. 8(12): e1003159 Polycomb controls gliogenesis by regulating the transient expression of the gcm/glide fate determinant. [FBrf0220515] Smibert et al., 2012, Cell Rep. 1(3): 277--289 Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila. [FBrf0218523] Weidmann and Goldstrohm, 2012, Mol. Cell. Biol. 32(2): 527--540 Drosophila Pumilio Protein Contains Multiple Autonomous Repression Domains That Regulate mRNAs Independently of Nanos and Brain Tumor. [FBrf0217089] Xiao et al., 2012, Development 139(15): 2670--2680 klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division. [FBrf0218804] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Harris et al., 2011, Dev. Cell 20(1): 72--83 Brat Promotes Stem Cell Differentiation via Control of a Bistable Switch that Restricts BMP Signaling. [FBrf0212787] Hilgers et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15864--15869 Neural-specific elongation of 3' UTRs during Drosophila development. [FBrf0215804] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Zhu et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(51): 20615--20620 Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains. [FBrf0216951] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...