FB2021_06, released December 14, 2021
Gene: Dmel\Gsc
Gene: Dmel\Gsc
D-gsc, l(2)05341, 60Mun2, Muenster 72
transcription factor - homeodomain - paired-like - regulates cell fate in the brain and stomatogastric nervous system
Please see the JBrowse view of Dmel\Gsc for information on other features
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Unconventional translation start (AUU) postulated; FlyBase analysis.
Gene model reviewed during 5.52
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 6.03
2.4 (longest cDNA)
None of the polypeptides share 100% sequence identity.
419 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gsc using the Feature Mapper tool.
Gsc transcript is expressed in two domains during embryogenesis. At stage 4, Gsc transcript is expressed as a narrow dorsoanterior stripe, which assumes a horseshoe shape by stage 6. The horseshoe splits dorsally at stage 8, and by stage 14, cells expressing Gsc transcript are located in the brain hemispheres. The second domain of expression begins at stage 9 at the anterior tip of the embryo, in cells which invaginate into the stomodeum. These cells abut the anterior midgut region at the end of germband retraction, and might contribute to the anterior foregut, the ring gland, and the stomodeal nervous system.
Gsc transcripts are first detected in a horseshoe pattern in the procephalic neurogenic region of blastoderm embryos. Later these cells delaminate in two groups and form a ventro-lateral sector of each brain hemisphere. At stage 10, a new expression domain appears in the foregut anlage and shortly after marks the stomodeum. This expression domain gives rise to a specific region of the foregut at the junction of the pharynx and esophagus, a part of the stomatogastric nervous system, and a part of the ring gland.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Gsc in GBrowse 22-1.5
2-0.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Gsc protein behaves as a transcriptional repressor, acting through specific palindromic homeodomain sites (P3K sites). Gsc protein is a "passive repressor" of activator homeoproteins binding to the same sites and an "active repressor" of activators binding to distinct sites. Gsc protein is also able to strongly repress transcription activated by Paired-class homeoproteins through P3K sites, via specific protein-protein interactions. This "interactive repression" requires the conserved GEH/eh-1 domain.
The sequence used to clone Gsc as reported in FBrf0087230 was '60Mun2', not Pph13 (60Mun1).
'60Mun1' as stated in FBrf0087230 should actually have read '60Mun2' (see FBrf0100819).
Gsc is required for the birth, survival or invagination (detachment from the foregut anlage) of EG1 precursor cells.
Mutant analysis suggests that Gsc protein regulates regional development of specific tissues.
