Rac, Drac1, Rac GTPase, Drac, D-Rac1
GTPase of the Ras superfamily - regulates cytoskeletal dynamics - controls epithelial tube length through the apical secretion and polarity pathways - Drk/Dos/Sos converge with Crk/Mbc/dCed-12 to activate Rac1 during glial engulfment of axonal debris - Rac1 acts downstream of integrin to control collective migration and lumen size in the Drosophila salivary gland
Gene model reviewed during 5.45
Interacts with Sra-1. Interacts (via REM 1 repeats) with Pkn (via N-terminus).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rac1 using the Feature Mapper tool.
Rac1 transcripts are detected throughout development with no significant fluctuations in transcript levels.
Rac1 is expressed throughout embryogenesis. After gastrulation, highest expression is detected in the mesoderm, the CNS and the hindgut primordium. Later expression is more uniform.
Rac1 transcripts are detected throughout development. In embryos, strong ubiquitous expression is observed in blastoderm stages. The transcript is concentrated at the basal part of the cellular blastoderm. After gastrulation, transcripts become highly enriched in the somatic mesoderm. Transcripts start to appear in the CNS and gut in stage 13. Later, somatic mesoderm expression vanishes but gut and CNS expression persists.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Rac1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Rac1 CG2248
Rac1 contributes to both passive memory decay and interference-induced forgetting.
Rac1 is needed in wild-type cells for the death of Minute neighbours in cell competition experiments.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Rac1 appears to be required for the regulation of actin polymerisation and/or myosin activity that is critical for the response of growth cones to midline repulsive signals.
Reduced RacGAP50C or increased Rac1 activity in the wing disc cause similar defects: widening of veins, development of extra sensory organs, apoptosis and appearance of enlarged cells that differentiate multiple hairs with abnormal polarity.
ISNb growth cones and SNa axons require Rac1 for guidance to appropriate targets.
Light and electron microscopy are used to study the process of myoblast fusion in Rac1 mutants.
Expression of dominant inhibitory Rac1 disrupts the epidermal cell shape changes of dorsal closure by preventing a localised accumulation of actin and myosin that normally occurs in the epidermal cells flanking the amnioserosa. The accumulation of actin and myosin along the dorsal side of these cells is believed to drive the cell shape changes of dorsal closure.
Both Rac1 and Rac2 proteins are marginally more like human Rac1 and Rac2, respectively, but the DNA sequences encoding them are more homologous to human Rac2 cDNA than that of human Rac1. This data does not readily allow the relationship between Rac1 and Rac2 and the genes encoding human Rac1 and Rac2 to be determined. Because of this the authors decided to assign new genes names using letters instead of 1 and 2 designators.