FlyBase Gene Report: Dmel\Taf1

General Information

Symbol

Dmel\Taf1

Species

D. melanogaster

Name

TBP-associated factor 1

Annotation Symbol

CG17603

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010355

Gene Model Status

Current

Stock Availability

13 publicly available

Enzyme Name (EC)

Histone acetyltransferase (2.3.1.48)

E1 ubiquitin-activating enzyme (6.2.1.45)

Gene Snapshot

TBP-associated factor 1 (Taf1) encodes a component of the evolutionarily conserved general transcription factor TFIID. It regulates transcription by binding the initiator element at transcription start sites and binding acetylated histones via its bromodomains. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

dTAF_II250, dTAF_II230, TAF_II250, Taf250, TAF_II230

Key Links

Genomic Location

Cytogenetic map

84A1-84A1

Sequence location

3R:6,646,889..6,656,048 [+]

Recombination map

3-48

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

E2 UBIQUITIN CONJUGATING ENZYMES

ATYPICAL PROTEIN KINASES

LYSINE N-ACETYLTRANSFERASES

TRANSCRIPTION FACTOR II D

HISTONE ACETYLTRANSFERASES

Protein Family (UniProt)

Belongs to the TAF1 family. (P51123)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

H4 histone acetyltransferase activity; histone acetyltransferase activity (H3-K14 specific); contributes_tocontributes_tocontributes_to RNA polymerase II general transcription initiation factor activity; sequence-specific DNA binding; transcription factor binding; contributes_to transcription regulatory region DNA binding; ubiquitin activating enzyme activity; ubiquitin conjugating enzyme activity

Predictions / Assertions

histone acetyltransferase activity; protein serine/threonine kinase activity; sequence-specific DNA binding; TBP-class protein binding

Catalytic Activity (EC)

Experimental Evidence

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine (2.3.1.48)

ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine (6.2.1.45)

Predictions / Assertions

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine (2.3.1.48)

Summaries

Gene Group (FlyBase)

E2 UBIQUITIN CONJUGATING ENZYMES -: Ubiquitin-conjugating (E2) enzymes accept activated ubiquitin from ubiquitin-activating (E1) enzymes via thioester bond with a conserved catalytic cysteine. E2s then interact with ubiquitin ligases (E3) to directly or indirectly ubiquitinate target proteins. E2s are characterised by a 200 amino acid ubiquitin-conjugating (UBC) domain which interacts with E1 and E3s and acts catalytically. (Adapted from PMID:1994026).
ATYPICAL PROTEIN KINASES -: Atypical kinases are protein kinases or putative protein kinases that do not share clear sequence similarity with conventional eukaryotic protein kinases, but they or their orthologs have been shown experimentally to have protein kinase activity. Atypical kinases are defined in FBrf0201870.
LYSINE N-ACETYLTRANSFERASES -: Lysine (K) acetyltransferases (KAT) transfer an acetyl group to ε-amino group of lysine residues. KATs have a long-established role as histone acetylases (HAT), but are now known to act on many non-histone proteins. (Adapted from FBrf0159755 and PMID:21075636).
TRANSCRIPTION FACTOR II D -: The general transcription factor complex Transcription factor II D (TFIID) plays an important role in recruiting the transcription machinery to core promoters. TFIID is a complex composed of TBP and several TBP-associated factors. (Adapted from FBrf0125116 and FBrf0156065).
HISTONE ACETYLTRANSFERASES -: Histone acetyltransferases catalyze the acetylation of conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyl lysine. (Adapted from PMID:11395403).

Protein Function (UniProtKB)

TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Largest component and core scaffold of the complex. Contains N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Negative regulator of the TATA box-binding activity of Tbp.

(UniProt, P51123)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(3)84Ab

Cell lethal; dies as normal-appearing second instar larva.

Summary (Interactive Fly)

component of the conserved general transcription factor TFIID - kinase and histone transacetylase activities - regulates transcription by binding the initiator element at transcription start sites and binding acetylated histones via its bromodomains

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Taf1 or the JBrowse view of Dmel\Taf1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Taf1-RA

FBtr0081684

NM_057608

6552

2065

Taf1-RB

FBtr0081685

NM_206438

6744

2129

Taf1-RC

FBtr0081686

NM_206437

6651

2098

Taf1-RD

FBtr0301622

NM_001170061

6645

2096

Taf1-RE

FBtr0304903

NM_001260023

6873

2172

Taf1-RF

FBtr0345257

NM_001300272

6648

2097

Additional Transcript Data and Comments

Reported size (kB)

6.552 (compiled cDNA)

(Kokubo et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Taf1-PA

FBpp0081184

232.2

2065

4.92

NP_476956

AAF54102

Taf1-PB

FBpp0089369

239.3

2129

4.92

NP_996160

AAS65116

Taf1-PC

FBpp0089420

236.0

2098

4.99

NP_996159

AAS65117

Taf1-PD

FBpp0290837

235.6

2096

4.86

NP_001163532

ACZ94830

Taf1-PE

FBpp0293442

244.1

2172

4.93

NP_001246952

AFH06271

Taf1-PF

FBpp0311434

235.6

2097

4.86

NP_001287201

AHN57200

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

2068 (aa); 232 (kD predicted)

(Kokubo et al., 1993)

250 (kD)

(Weinzierl et al., 1993)

Comments

External Data

Subunit Structure (UniProtKB)

Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (Tafs). Taf1 is the largest component of the TFIID complex. Interacts with Tbp, Taf2, Taf4 and Taf6.

(UniProt, P51123)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1TBA

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Taf1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Taf1_1

Linkouts

Gene Ontology (24 terms)

Molecular Function (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

H4 histone acetyltransferase activity

inferred from direct assay

(Mizzen et al., 1996)

histone acetyltransferase activity (H3-K14 specific)

inferred from direct assay

(Mizzen et al., 1996)

contributes_tocontributes_tocontributes_to RNA polymerase II general transcription initiation factor activity

inferred from direct assay

(Metcalf and Wassarman, 2006, Wright et al., 2006, Verrijzer et al., 1995)

sequence-specific DNA binding

inferred from direct assay

(Metcalf and Wassarman, 2006)

transcription factor binding

inferred from physical interaction with FLYBASE:Taf12; FB:FBgn0011290

(Yokomori et al., 1993)

inferred from physical interaction with FLYBASE:Taf11; FB:FBgn0011291

(Yokomori et al., 1993)

contributes_to transcription regulatory region DNA binding

inferred from direct assay

(assigned by BHF-UCL )

(Verrijzer et al., 1995)

ubiquitin activating enzyme activity

inferred from direct assay

(Pham and Sauer, 2000)

ubiquitin conjugating enzyme activity

inferred from direct assay

(Pham and Sauer, 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

histone acetyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000356981

(assigned by GO_Central )

(Gaudet et al., 2011)

protein serine/threonine kinase activity

inferred from sequence or structural similarity with HGNC:11535

(FlyBase Curators, 2008--)

sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000356981

(assigned by GO_Central )

(Gaudet et al., 2011)

TBP-class protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000356981

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

DNA-templated transcription, initiation

inferred from direct assay

(assigned by BHF-UCL )

(Verrijzer et al., 1995)

histone H3-K14 acetylation

inferred from direct assay

(Mizzen et al., 1996)

histone H4 acetylation

inferred from direct assay

(Mizzen et al., 1996)

histone monoubiquitination

inferred from direct assay

(Pham and Sauer, 2000)

positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Wright et al., 2006)

positive regulation of transcription initiation from RNA polymerase II promoter

inferred from direct assay

(assigned by BHF-UCL )

(Verrijzer et al., 1995)

RNA polymerase II preinitiation complex assembly

inferred from direct assay

(assigned by BHF-UCL )

(Verrijzer et al., 1995)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000356981

(assigned by GO_Central )

(Gaudet et al., 2011)

protein phosphorylation

inferred from sequence or structural similarity with HGNC:11535

(FlyBase Curators, 2008--)

transcription initiation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC:11535

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000001039

(Aoyagi and Wassarman, 2000)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

nucleolus

inferred from direct assay

(Metcalf and Wassarman, 2007)

nucleoplasm

inferred from direct assay

(Metcalf and Wassarman, 2007)

nucleus

inferred from direct assay

(Wright et al., 2006, Hansen and Tjian, 1995)

transcription factor TFIID complex

inferred from physical interaction with FLYBASE:Taf4; FB:FBgn0010280

(Wright et al., 2006)

inferred from physical interaction with FLYBASE:Taf6; FB:FBgn0010417

(Wright et al., 2006)

inferred from direct assay

(Hansen and Tjian, 1995)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

transcription factor TFIID complex

inferred from biological aspect of ancestor with PANTHER:PTN000356981

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with HGNC:11535

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000001039

(Aoyagi and Wassarman, 2000)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Metcalf and Wassarman, 2007)

spermatocyte

(Metcalf and Wassarman, 2007)

spermatogonium

(Metcalf and Wassarman, 2007)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Taf1 protein is first detected in mitotically profliferating spermatogonia at the apical tip of the testis. Taf1 levels increases in spermatocytes and reaches the highest levels in nuclei of spermatocytes undergoing growth in an extended pre-meiotic G2 phase. Upon entry into meiosis, expression is reduced and the Taf1 protein is dispersed throughout the cell. @Taf1 is dynamically localized within the nucleus during spermatogenesis and predominantly localizes to spermatocyte nucleoli.

(Metcalf and Wassarman, 2007)

Marker for

Subcellular Localization

CV Term

Evidence

References

nucleolus

inferred from direct assay

(Metcalf and Wassarman, 2007)

nucleoplasm

inferred from direct assay

(Metcalf and Wassarman, 2007)

nucleus

inferred from direct assay

(Wright et al., 2006, Hansen and Tjian, 1995)

transcription factor TFIID complex

inferred from physical interaction with FLYBASE:Taf4; FB:FBgn0010280

(Wright et al., 2006)

inferred from physical interaction with FLYBASE:Taf6; FB:FBgn0010417

(Wright et al., 2006)

inferred from direct assay

(Hansen and Tjian, 1995)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Taf1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Taf1

View exonic expression by tissue for Dmel\Taf1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010355

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010355

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG17603-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0010355

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Taf1

Transgenic constructs containing regulatory region of Taf1

Deletions and Duplications ( 25 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Taf1^GD4594

lethal | recessive

lethal - all die before end of larval stage | cell autonomous | recessive

Taf1¹

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Taf1^GD4594

partially lethal - majority die

Taf1^XS-2232

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Taf1^GD4594

viable, with Scer\GAL4^- :

Taf1^EP421

viable, with Scer\GAL4^tin.CΔ4

Taf1^GD4594

Sterility

Allele

female sterile

female sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Other Phenotypes

Allele

neuroanatomy defective | larval stage

Taf1^GD4594

some die during larval stage | recessive

Taf1¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

Taf1^GD4594

visible, with Scer\GAL4^bbg-C96

Taf1^{dsRNA.UAS.cUa}

visible, with Scer\GAL4^pnr-MD237

Taf1^GD4594

Phenotype manifest in

Allele

egg chamber

Taf1^S-625

egg chamber, with Scer\GAL4^- :

Taf1^EP421

embryonic/larval fat body | wandering third instar larval stage, with Scer\GAL4^ppl.PP

eye photoreceptor cell

Taf1^XS-2232

eye photoreceptor cell, with Scer\GAL4^- :

Taf1^EP421

ganglion mother cell | larval stage

Taf1^GD4594

interommatidial bristle

Taf1^XS-2232

interommatidial bristle, with Scer\GAL4^- :

Taf1^EP421

larval neuroblast | larval stage

Taf1^GD4594

lipid droplet | wandering third instar larval stage, with Scer\GAL4^ppl.PP

macrochaeta

macrochaeta, with Scer\GAL4^- :

Taf1^EP421

male terminalia

Taf1^XS-2232

male terminalia, with Scer\GAL4^- :

Taf1^EP421

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Taf1^GD4594

microchaeta

Taf1^XS-2232

microchaeta, with Scer\GAL4^- :

Taf1^EP421

ocellus

Taf1^XS-2232

ocellus, with Scer\GAL4^- :

Taf1^EP421

oocyte

Taf1^XS-2232

oocyte, with Scer\GAL4^- :

Taf1^EP421

ovary

Taf1^XS-2232

ovary, with Scer\GAL4^- :

Taf1^EP421

wing

Taf1^XS-2232

wing, with Scer\GAL4^- :

Taf1^EP421

wing margin, with Scer\GAL4^bbg-C96

Taf1^{dsRNA.UAS.cUa}

wing vein L4

Taf1^XS-2232

wing vein L4, with Scer\GAL4^- :

Taf1^EP421

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TAF1

14 of 15

Yes

Hsap\TAF1L

13 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Taf1

13 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Taf1

2 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\taf1

8 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\taf1

13 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\taf-1

13 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\HAF01

6 of 9

Yes

Atha\HAF2

6 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TAF1

10 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\taf111

7 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900EI )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Taf1

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14575

Drosophila persimilis

Dper\GL21765

Drosophila willistoni

Dwil\GK12244

Drosophila virilis

Dvir\GJ26499

Drosophila mojavensis

Dmoj\GI23838

Drosophila grimshawi

Dgri\GH18122

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500AF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17013821

Glossina morsitans

Tsetse fly

Gmor\GMOY002155

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_01282

Mayetiola destructor

Hessian fly

Mdes\Mdes000109

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006082

Anopheles darlingi

American malaria mosquito

Adar\ADAC004135

Anopheles gambiae

Malaria mosquito

Agam\AGAP003882

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003658

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00FD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007052

Danaus plexippus

Monarch butterfly

Dple\DPOGS200534-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL009373

Apis florea

Little honeybee

Aflo\3430962676

Apis mellifera

Western honey bee

Amel\GB44341

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19543

Bombus terrestris

Buff-tailed bumblebee

Bter\15209928

Linepithema humile

Argentine ant

Lhum\LH23087

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000d9a

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013825

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05148

Tribolium castaneum

Red flour beetle

Tcas\TC002507

Pediculus humanus

Human body louse

Phum\PHUM537430

Rhodnius prolixus

Kissing bug

Rpro\RPRC001858

Cimex lectularius

Bed bug

Clec\CLEC000253

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI56611

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01981

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00ED )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005203

Strigamia maritima

European centipede

Smar\SMAR007466

Ixodes scapularis

Black-legged tick

Isca\ISCW012742

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77739.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM82605.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur18g01640

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_10162

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00MO )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Taf1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Taf1l_1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000024506

Gallus gallus

Domestic chicken

Ggal\TAF1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TAF1; TATA-box binding protein associated factor 1

model of X-linked dystonia-parkinsonism

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TAF1; TATA-box binding protein associated factor 1

14 of 15

313650

X-linked dystonia-parkinsonism

TAF1L; TATA-box binding protein associated factor 1 like

13 of 15

607798

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Taf1 - Bap60

ion exchange chromatography, molecular sieving, western blot

(Vorobyeva et al., 2009)

Taf1 - Stat92E

anti bait coimmunoprecipitation, western blot

(Panov et al., 2012)

Taf1 - Taf10

anti bait coimmunoprecipitation, western blot, affinity chromatography technology

(Santoso and Kadonaga, 2006, Georgieva et al., 2001)

Taf1 - Taf11

anti bait coimmunoprecipitation, western blot

(Yokomori et al., 1993)

Taf1 - Taf12

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Yokomori et al., 1993)

Taf1 - Taf2

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, autoradiography, affinity chromatography technology

(Rhee et al., 2014, Santoso and Kadonaga, 2006, Verrijzer et al., 1994)

Taf1 - Taf4

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, affinity chromatography technology

(Vorobyeva et al., 2009, Santoso and Kadonaga, 2006, Wright et al., 2006, Kokubo et al., 1993, Yokomori et al., 1993)

Taf1 - Taf5

affinity chromatography technology, western blot, anti bait coimmunoprecipitation

(Vorobyeva et al., 2009, Santoso and Kadonaga, 2006)

Taf1 - Taf6

affinity chromatography technology, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation

(Weake et al., 2009, Santoso and Kadonaga, 2006, Wright et al., 2006)

Taf1 - TfIIA-S

anti bait coimmunoprecipitation, western blot

(Yokomori et al., 1994)

Taf1 - e(y)1

anti bait coimmunoprecipitation, western blot, affinity chromatography technology, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009, Santoso and Kadonaga, 2006, Goodrich et al., 1993)

Taf1 - e(y)2

anti bait coimmunoprecipitation, western blot

(Georgieva et al., 2001)

Taf1 - e(y)3

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009, Vorobyeva et al., 2008)

Taf1 - mor

anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009)

Taf1 - ph-p

anti tag coimmunoprecipitation, ion exchange chromatography, molecular sieving, western blot, peptide massfingerprinting

(Saurin et al., 2001)

Taf1 - polybromo

ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation

(Vorobyeva et al., 2009)

Taf1 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Taf1

(Pham and Sauer, 2000)

Ras85D

suppressible

Taf1

(Karim et al., 1996)

Ras85D|sev

suppressible

Taf1

(Maixner et al., 1998)

External Data

Subunit Structure (UniProtKB)

(UniProt, P51123 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66011

DroID - A comprehensive database of gene and protein interactions.

FBgn0010355

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40813

MIST (genetic) - An integrated Molecular Interaction Database

Taf1

MIST (protein-protein) - An integrated Molecular Interaction Database

Taf1

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84A1-84A1

Sequence location

3R:6,646,889..6,656,048 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84A1-84A1

Limits computationally determined from genome sequence between P{lacW}cas^j1C2 and P{PZ}lab⁰¹²⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84A-84A

(Karim et al., 1996)

84A2-84A3

(determined by in situ hybridisation)

(Weinzierl et al., 1993)

Deduced from meiotic mapping.

(Karim et al., 1996)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

(Karim et al., 1996)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Bloomington

56689

y¹ w^*; Mi{MIC}Taf1^MI11749

17281

w¹¹¹⁸; P{EP}Taf1^EP421/TM3, Sb¹

5300

Taf1¹ red¹ e¹/TM3, Sb¹

35314

y¹ sc^* v¹ sev²¹; P{TRiP.GL00219}attP2

78650

y¹ sc^* v¹ sev²¹; P{TOE.GS01898}attP40

32421

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00416}attP2

67934

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05766}attP40

Harvard

e02697

PBac{RB}Taf1^e02697

Kyoto

108159

Taf1¹ red¹ e¹/TM3, Sb¹

106647

y¹; Taf1^R14 red¹ e¹/TM2

VDRC

v106119

P{KK100418}VIE-260B

v41099

w¹¹¹⁸; P{GD4594}v41099/TM3

More stocks available...

Genomic Clones (30)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

FI22504

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010355

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010355

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40813

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Weinzierl et al., 1993)

polyclonal

(Kokubo et al., 1993)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Taf250 cell

(Wassarman, 1999.8.24)

Source for merge of: Taf250 SR3-5

(Wassarman et al., 2000)

Additional comments

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Marr et al., 2006)

The proteins encoded by Tbp, Taf1, Taf2 and Taf11 are likely peripheral subunits of TFIID.

(Wright et al., 2006)

The proteins encoded by Taf1 and Taf4 have a specific role in transcription activation from TATA-less, downstream core promoter element (DPE)-containing promoters.

(Wright et al., 2006)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, an increase in the proportion of G2/M phase cells, a decrease in mitotic index and a whole range of mitotic abnormalities are seen.

(Bettencourt-Dias et al., 2004)

Taf1 is a histone specific ubiquitin activating/conjugating enzyme (ubac).

(Pham and Sauer, 2000)

Taf1 is required in ovary, eye, ocelli, wing bristle and terminalia development and has a suggested role in regulating the cell cycle, cell differentiation, cell proliferation and cell survival.

(Wassarman et al., 2000)

Transcriptional regulation may involve, in part, competitive interactions between transcriptional activators and TAFs on the Tbp surface.

(Nishikawa et al., 1997)

Identified on the basis of genetic interaction with Ras85D^V12.sev.

(Karim et al., 1996)

Taf1 exhibits histone acetyltransferase (HAT) activity in vitro.

(Mizzen et al., 1996)

A quadruple complex containing Tbp, Taf1, Taf4 and Taf6 mediates transcriptional synergism by bcd and hb, whereas triple Tbp-Taf complexes lacking one or other coactivator failed to support synergistic activation. The concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during development.

(Sauer et al., 1995)

The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro.

(Chen et al., 1994)

TFIID subunit proteins and the Tbp protein can interact in pairwise combinations with several subunits in a network of interactions within TFIID.

(Kokubo et al., 1994)

N terminal sites of Taf1 mediate strong physical interaction with Tbp and inhibit Tbp function. Interaction of the N terminal region with Tbp may directly control TATA-box binding.

(Kokubo et al., 1994)

Taf1 contains at least two sites of interaction with Tbp.

(Kokubo et al., 1994)

Purified Taf5 is unable to bind Tbp directly or to interact strongly with the C-terminal domain of Taf1, but can only interact with a complex containing Tbp, Taf1, Taf4 and Taf6.

(Dynlacht et al., 1993)

Taf1 gene product interacts directly with Tbp.

(Kokubo et al., 1993)

A number of polypeptides (encoded by Taf1, Taf4, Taf5, Taf6, e(y)1 and Taf12) that are tightly associated with Tbp and are native TFIID components have been purified. Protein blotting experiments suggest that one of these proteins, Taf1, interacts directly with Tbp, while the association between Tbp and the other proteins is either weak or is an indirect association via Taf1.

(Kokubo et al., 1993)

In vitro expressed 'TAF110' interacts directly with the 'TAF250' which itself interacts with Tbp, a complex of all three is suggested. Binding study with various mutants of Taf1 suggests that both 'TAF110' and Tbp interact with the N-terminal 352-amino acid portion of 'TAF250'.

(Kokubo et al., 1993)

The Taf6 product interacts directly with Taf1 in vitro.

(Weinzierl et al., 1993)

Tbp, Taf1, Taf4 and Taf6 products can assemble into a mini-complex in vitro, showing that Taf6 and Taf4 occupy distinct binding sites on the C terminal part of Taf1.

(Weinzierl et al., 1993)

The assembly of a partial complex containing recombinant Tbf1, Taf1 and Taf4 was reported. This triple complex supports activation of HeLa cell Sp1 and reveals specific interactions between Tbp, Taf1 and Taf4.

(Weinzierl et al., 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 74 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40813

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P51123

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010355

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010355

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Taf1_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0010355

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40813

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002507

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG17603

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Basal

modMine - A data warehouse for the modENCODE project

FBgn0010355

PDB - An information portal to biological macromolecular structures

1TBA

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Linkouts

BioGRID - A database of protein and genetic interactions.

66011

DroID - A comprehensive database of gene and protein interactions.

FBgn0010355

DRSC - Results frm RNAi screens

FBgn0010355

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010355

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG17603-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0010355

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40813

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Basal transcription factors

MIST (genetic) - An integrated Molecular Interaction Database

Taf1

MIST (protein-protein) - An integrated Molecular Interaction Database

Taf1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (47)

Reported As

Symbol Synonym

BG:DS00004.13

CG17603

(Fan et al., 2017, Saha et al., 2016, Fukuoh et al., 2014, Page et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Metcalf and Wassarman, 2006, Aoyagi and Wassarman, 2000)

EfW1

(Hazelrigg and Kaufman, 1983)

SR3-5

(Wassarman et al., 2000, Maixner et al., 1998, Karim et al., 1996)

TAF

(Müller and Tora, 2004)

TAF200

(Hoey et al., 1993)

TAF230

(Kouzarides, 1999, Grunstein, 1997, Farmer et al., 1996)

TAF250

(Usakin et al., 2006, Kenyon et al., 2001, Park et al., 2001, Aoyagi et al., 2000, Wassarman et al., 2000, Aoyagi and Wassarman, 1999, Martin, 1999.1.14, Hansen et al., 1997)

TAF250/230

(Aoyagi and Wassarman, 2000, Green, 2000)

TAF_II

(Arnosti, 2002)

TAF_II230

(Lebedeva, 2005, Lebedeva et al., 2005, Yang, 2004, Leibovitch et al., 2002, Tora, 2002, Wassarman and Sauer, 2001)

TAF_II250

(Conaway et al., 2002, Wassarman and Sauer, 2001, Mizzen and Allis, 2000, Pham et al., 1999, Schock et al., 1999, Aravind and Landsman, 1998, Cutler et al., 1998, Naar et al., 1998, Jeanmougin et al., 1997, Niessing et al., 1997, Tsukiyama and Wu, 1997, Hansen and Tjian, 1995)

TAF_II250/230

(Shen et al., 1998, Tansey and Herr, 1997)

TAF^II250

(Kennett et al., 2002)

TAFII-250

(Jones and Kadonaga, 2000)

TAFII250

(Schwartz and Pirrotta, 2007, Wassarman et al., 2001, Dynlacht et al., 1993, Weinzierl et al., 1993)

TFIID

(Butler and Kadonaga, 2002, Gu et al., 2002, Hochheimer et al., 2002, Simon and Tamkun, 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001)

TFIID TAF250

(Berk, 2000)

Taf1p

(Kasahara et al., 2019, Kasahara et al., 2004)

Taf200

Taf230

(Richardson Rounds and Petri, 1996)

Taf250

(Bell and Tora, 1999, Wassarman, 1999.8.24, Struhl and Moqtaderi, 1998, Sauer et al., 1995, Verrijzer et al., 1995, Hori and Carey, 1994, Kokubo et al., 1994, Verrijzer et al., 1994, Smith et al., 1993, Weinzierl et al., 1993)

Taf_II250

(Chen et al., 1994)

cel

(Kaufman et al., 1990, Merrill et al., 1989, Wakimoto et al., 1984)

cell

(Mohler, 1997)

d230

(Pugh, 2000)

dTAF1

(Pahi et al., 2015)

dTAF230

(Wu et al., 2001)

dTAF250

(Rabenstein et al., 1999)

dTAF_II230

(Wright et al., 2006, Georgieva et al., 2001, Pereira et al., 2001, Bagby et al., 2000, Georgieva et al., 2000, Burley and Roeder, 1998, Kotani et al., 1998, Kuo and Allis, 1998, Liu et al., 1998, Weissman et al., 1998, Nishikawa et al., 1997, Tsukiyama and Wu, 1997, Mizzen et al., 1996)

dTAF_II250

(Gangloff et al., 2001, Park et al., 2001, Veenstra and Wolffe, 2001, Albright and Tjian, 2000, Pham and Sauer, 2000, Pugh, 2000, Lee and Young, 1998, Zhou et al., 1998, Damania and Alwine, 1996, Goodrich and Tjian, 1994, Verrijzer et al., 1994, Goodrich et al., 1993, Yokomori et al., 1993)

dTAFII250

(Saurin et al., 2001, Yokomori et al., 1994)

dmTAF1

(Lebedeva, 2005, Lebedeva et al., 2005, Muratoglu et al., 2003)

dmTAF_II230

(Pandey et al., 2002)

l(3)84Ab

(Lindsley and Zimm, 1992)

p230

(Kokubo et al., 1994, Kokubo et al., 1993, Kokubo et al., 1993, Kokubo et al., 1993)

taf1

(Fan et al., 2017)

Name Synonyms

Suppressor of Ras85D 3-5

(Karim et al., 1996)

TATA-associated factor 250

(Weinzierl et al., 1993)

TBP-associated factor

(Schuettengruber et al., 2007)

TBP-associated factor 1

(Venables et al., 2012, Roy et al., 2007, Xing et al., 2007)

TBP-associated factor 250

(Dynlacht et al., 1993)

TBP-associated factor 250kD

TFIID 230 kda subunit

(Kokubo, 1993.7.21)

cell lethal

Secondary FlyBase IDs

FBgn0004775
FBgn0010420
FBgn0016735

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (206)

Report Sections

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