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Gene Dmel\Taf1

General Information
Symbol	Dmel\Taf1	Species	D. melanogaster
Name	TBP-associated factor 1	Annotation symbol	CG17603
Feature type	protein_coding_gene	FlyBase ID	FBgn0010355
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	TAF250, TAFII250, dTAFII230, dTAFII250, TAFII230, TFIID, TAFII250, SR3-5, TAF230, p230
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	84A1-84A1	Sequence location	3R:2,472,611..2,481,770 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TBP-associated factor 1 is referred to in FlyBase by the symbol Dmel\Taf1 (CG17603, FBgn0010355). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly; transcription factor binding; sequence-specific DNA binding; histone serine kinase activity; RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly. There is experimental evidence that it is involved in the biological process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription involved in G2/M-phase of mitotic cell cycle; regulation of JAK-STAT cascade; histone acetylation; neurogenesis. 31 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; adult; adult segment; sense organ; external compound sense organ; multicellular structure; thoracic segment; region of integument; ovariole. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: AT hook, DNA-binding motif; Bromodomain; Bromodomain, conserved site; TAFII-230 TBP-binding; Transcription initiation factor TFIID subunit 1, animal; Transcription initiation factor TFIID subunit 1, domain of unknown function; Zinc finger, CCHC-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1632256_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Taf1. Gene sequence location is 3R:2472611..2481770.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(3)84Ab Cell lethal; dies as normal-appearing second instar larva.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84A1-84A1   Limits computationally determined from genome sequence between P{lacW}casj1C2 and P{PZ}lab01241
Experimentally Determined Cytological Location
Cytogenetic map Notes References 84A2-84A3   (determined by in situ hybridisation)   (Weinzierl et al., 1993) 84A-84A   Deduced from meiotic mapping.   (Karim et al., 1996)
Experimentally Determined Recombination Data
Location	3- (Karim et al., 1996)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Taf1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Taf1-RA FBtr0081684  NM_057608   6552   2065 Taf1-RB FBtr0081685  NM_206438   6744   2129 Taf1-RC FBtr0081686  NM_206437   6651   2098 Taf1-RD FBtr0301622  NM_001170061   6645   2096 Taf1-RE FBtr0304903  NM_001260023   6873   2172
Additional Transcript Data & Comments
Reported size (kB)	6.552 (compiled cDNA) (Kokubo et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Taf1-PA   FBpp0081184   232.2   2065   4.92     NP_476956   AAF54102 Taf1-PB   FBpp0089369   239.3   2129   4.92     NP_996160   AAS65116 Taf1-PC   FBpp0089420   236.0   2098   4.99     NP_996159   AAS65117 Taf1-PD   FBpp0290837   235.6   2096   4.86     NP_001163532   ACZ94830 Taf1-PE   FBpp0293442   244.1   2172   4.93     NP_001246952   AFH06271
Additional Polypeptide Data & Comments
Reported size (kDa)	2068 (aa); 232 (kD predicted) (Kokubo et al., 1993) 250 (kD) (Weinzierl et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AT hook, DNA-binding motif (IPR017956) Bromodomain, conserved site (IPR018359) Bromodomain (IPR001487) TAFII-230 TBP-binding (IPR009067) Zinc finger, CCHC-type (IPR001878) Transcription initiation factor TFIID subunit 1, animal (IPR011177) Transcription initiation factor TFIID subunit 1, domain of unknown function (IPR022591) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1TBA
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141266   AA202346   AA246700   AA263181   AA392445   AA439081   AA567071   AA735391   AA802078   AA817407   AA949335   AA979324   AE014297 AAF54102   AE014297 AAS65116   AE014297 AAS65117   AC001652   AE014297 ACZ94830   AE001572 AAD19815   AE014297 AFH06271   AI387456   AI514408   AI514425   AI516439   AI516440   AQ025084   BF495700   BF499008   BG633158   BG638043   BG638993   BI231764   BI481542   BI580710   BT004888 AAO47866   BT024196   BT025855   CO181997   CO186121   CO186553   CO190747   CO192409   CO264850   CO274929   CO277457   CO284340   CO293219   CO298675   CO306986   CO312079   CO314183   CO314665   CO315965   CO322966   CO343308   CO343733   CZ480469   EC057361   EC065391   EC066421   EC083209   EC197479   EC200658   EC201210   EC202847   EC203545   EC236975   EC244336   EC248902   EC249779   EC255001   EC260568   EL875201   EL875202   EL884060   EL884061   EL884173   ES690161   ES690162   ES690193   ES691077   ES692212   ES692216   ES692218   ES692221   ES692224   ES692226   GH945788   GH946209   GH948206   GH949916   GH950541   GH951616   GH952338   GH952905   GH959532   GH961761   GH962601   GH964586   GH965204   GH966106   GH966194   GH967052   GH967765   GH970018   GH970808   GH973231   GH974483   GH976805   GH977705   GH980982   GH981274   GH982839   GH984819   S61883 AAB26991
UniProtKB/Swiss-Prot	P51123
UniProtKB/TrEMBL	E1JJ72
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Wright et al., 2006, Hansen and Tjian, 1995) transcription factor TFIID complex inferred from direct assay (Hansen and Tjian, 1995) inferred from physical interaction with Taf4 (Wright et al., 2006) inferred from physical interaction with Taf6 (Wright et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    97.2   NA  Eye    83.925   NA  Brain    188.8   272.65  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    149.8   NA  Crop    120.7   71.3  Midgut    80.3   107.9  Hindgut    84.6   107.4  Malpighian Tubules    126.9   112.9  Fat Body    114.8   164  Salivary Gland    184.8   NA  Heart    93.35   146.275  Trachea    NA   NA  VirginFemale Spermatheca    191.1   NA  InseminatedFemale Spermatheca    174.3   NA  Ovary    229.6   NA  Testis    108.3   NA  Male Accessory Gland    171.4   91.775  Carcass    98.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3    27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female    30 ovary, mated 4-day female    26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3  (27) central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female  (30) ovary, mated 4-day female  26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3    27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female    30 ovary, mated 4-day female    26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3    27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female    30 ovary, mated 4-day female    26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3    27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female    30 ovary, mated 4-day female    26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3  27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female  30 ovary, mated 4-day female  26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3    27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female    30 ovary, mated 4-day female    26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    22 central nervous system, larvae L3    27 central nervous system, pupae P8    16 head, virgin 1-day female    7 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    7 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    10 head, mated 4-day male    11 head, mated 20-day male    15 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    4 fat body, white prepupae    9 fat body, pupae P8    6 carcass, larvae L3 wandering    18 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    7 ovary, virgin 4-day female    30 ovary, mated 4-day female    26 testis, mated 4-day male    12 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    61 embryo 04-06hr    44 embryo 06-08hr    42 embryo 08-10hr    38 embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr    28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (40) embryo 02-04hr  (61) embryo 04-06hr  (44) embryo 06-08hr  (42) embryo 08-10hr  (38) embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr  (28) embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    61 embryo 04-06hr    44 embryo 06-08hr    42 embryo 08-10hr    38 embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr    28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    61 embryo 04-06hr    44 embryo 06-08hr    42 embryo 08-10hr    38 embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr    28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    61 embryo 04-06hr    44 embryo 06-08hr    42 embryo 08-10hr    38 embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr    28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (40) embryo 02-04hr  (61) embryo 04-06hr  (44) embryo 06-08hr  (42) embryo 08-10hr  (38) embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr  28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    61 embryo 04-06hr    44 embryo 06-08hr    42 embryo 08-10hr    38 embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr    28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    61 embryo 04-06hr    44 embryo 06-08hr    42 embryo 08-10hr    38 embryo 10-12hr    25 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    11 embryo 20-22hr    28 embryo 22-24hr    23 larva L1    11 larva L2    9 larva L3 12hr old    8 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    16 white prepupae 12hr    17 white prepupae 24hr    25 pupae 2d postWPP    15 pupae 3d postWPP    8 pupae 4d postWPP    6 adult male 01day    9 adult male 05day    12 adult male 30day    11 adult female 01day    15 adult female 05day    24 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1    26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS    31 ovary OSC    27 ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1  26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3  26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  (35) eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1  (27) CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS  (31) ovary OSC  (27) ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1    26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS    31 ovary OSC    27 ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1    26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS    31 ovary OSC    27 ovary OSS    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1    26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS    31 ovary OSC    27 ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1  26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3  26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3  (35) eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1  27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS  31 ovary OSC  27 ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1    26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS    31 ovary OSC    27 ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    18 embryonic 1182-4H    22 embryonic GM2    22 embryonic Kc167    16 embryonic S1    26 embryonic S3    17 leg disc CME L1    23 wing disc CME-W2    15 wing disc ML-DmD8    18 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    9 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    17 ovary fGS/OSS    31 ovary OSC    27 ovary OSS    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • RE63802 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010355 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17603-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Taf1GD4594 lethal | cell autonomous | larval stage | recessive Taf11 lethal | recessive Taf11 Taf12 Taf13 Taf14 Taf15 Taf16 Taf17 Taf18 Taf19 Taf110 Taf111 Taf112 Taf113 Taf114 Taf115 Taf1CTK Taf1R14 Taf1S-136 Taf1XS-2232 partially lethal - majority die Taf1XS-2232 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Taf1GD4594 viable, with Scer\GAL4[-]: Taf1EP421 Sterility Allele female sterile Taf1S-136 Taf1S-625 Taf1XS-2232 Other Phenotypes Allele mitotic cell cycle defective Taf1dsRNA.cMa neuroanatomy defective | larval stage Taf1GD4594 visible, with Scer\GAL4pnr-MD237 Taf1GD4594 Phenotype manifest in Allele egg chamber Taf1S-625 egg chamber, with Scer\GAL4[-]: Taf1EP421 embryonic/larval neuroblast | larval stage Taf1GD4594 eye photoreceptor cell Taf1XS-2232 eye photoreceptor cell, with Scer\GAL4[-]: Taf1EP421 ganglion mother cell | larval stage Taf1GD4594 interommatidial bristle Taf1XS-2232 interommatidial bristle, with Scer\GAL4[-]: Taf1EP421 macrochaeta Taf1S-136 Taf1S-625 Taf1XS-2232 macrochaeta, with Scer\GAL4[-]: Taf1EP421 male terminalia Taf1XS-2232 male terminalia, with Scer\GAL4[-]: Taf1EP421 mesothoracic tergum, with Scer\GAL4pnr-MD237 Taf1GD4594 microchaeta Taf1XS-2232 microchaeta, with Scer\GAL4[-]: Taf1EP421 ocellus Taf1XS-2232 ocellus, with Scer\GAL4[-]: Taf1EP421 oocyte Taf1XS-2232 oocyte, with Scer\GAL4[-]: Taf1EP421 ovary Taf1XS-2232 ovary, with Scer\GAL4[-]: Taf1EP421 second instar larva Taf11 wing Taf1XS-2232 wing, with Scer\GAL4[-]: Taf1EP421 wing vein L4 Taf1XS-2232 wing vein L4, with Scer\GAL4[-]: Taf1EP421
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of Taf1 Class Mutagen Stocks Known lesion Taf11 ethyl methanesulfonate 2 -- Taf1R14 ethyl methanesulfonate 2 -- Taf1e02697 piggyBac transposase 1 -- Taf1EP421 P-element activity 1 Yes Taf110 ethyl methanesulfonate 0 -- Taf111 ethyl methanesulfonate 0 -- Taf112 ethyl methanesulfonate 0 -- Taf113 ethyl methanesulfonate 0 -- Taf114 X ray 0 -- Taf115 ethyl methanesulfonate 0 -- Taf12 ethyl methanesulfonate 0 -- Taf13 X ray 0 -- Taf14 ethyl methanesulfonate 0 -- Taf15 ethyl methanesulfonate 0 -- Taf16 ethyl methanesulfonate 0 -- Taf17 ethyl methanesulfonate 0 -- Taf18 ethyl methanesulfonate 0 -- Taf19 ethyl methanesulfonate 0 -- Taf1CTK 0 Yes Taf1EP421-EX1 P-element activity 0 Yes Taf1S-136 ethyl methanesulfonate 0 Yes Taf1S-625 0 Yes Taf1XS-2232 X ray 0 Yes
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Taf1 Class Mutagen Stocks Known lesion Taf1GD4594 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1GL00219 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1HMS00416 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1KK100418 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1Act5C.PF in vitro construct - regulatory fusion 0 Yes Taf1dsRNA.cMa in vitro construct - RNAi 0 Yes Taf1dsRNA.cWa in vitro construct - RNAi 0 Yes Taf1NIG.17603R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Antp-X1 (Pham et al., 1999, Hazelrigg and Kaufman, 1983) Df(3R)Antp-X2 (Hazelrigg and Kaufman, 1983) Df(3R)BD5 (Wassarman et al., 2000) Df(3R)BSC738 (Christensen et al., 2009.2.28) Df(3R)Dfd13 (Hazelrigg and Kaufman, 1983) Df(3R)MAP2 (Merrill et al., 1989) Df(3R)Scr (Wakimoto et al., 1984, Merrill et al., 1989, Kaufman et al., 1990, Lewis et al., 1980) Df(3R)TplJE5 (Smoyer et al., 2003) T(2;3)VD3
Not disrupted in	Df(3R)Antp17 (Wakimoto et al., 1984, Kaufman et al., 1990, Lewis et al., 1980) Df(3R)Hu (Hazelrigg and Kaufman, 1983) Df(3R)MAP11 (Merrill et al., 1989) Df(3R)Scx2 (Hazelrigg and Kaufman, 1983, Wakimoto et al., 1984, Kaufman et al., 1990) Df(3R)Scx3 (Hazelrigg and Kaufman, 1983) Df(3R)Scx4 (Hazelrigg and Kaufman, 1983, Kaufman et al., 1990) Dp(3;3)Antp73b-rv8 (Hazelrigg and Kaufman, 1983) In(3R)Antprv9 (Hazelrigg and Kaufman, 1983) In(3R)ScrWrv5 (Hazelrigg and Kaufman, 1983) T(2;3)Antprv5 (Hazelrigg and Kaufman, 1983) T(2;3)Antprv6 (Hazelrigg and Kaufman, 1983) T(2;3)Antprv7 (Hazelrigg and Kaufman, 1983) T(2;3)ScrWrv1 (Hazelrigg and Kaufman, 1983) T(2;3)ScrWrv6 (Hazelrigg and Kaufman, 1983) Tp(3;3)Dfdrv1 (Hazelrigg and Kaufman, 1983) Tp(3;3)Dfdrv3 (Hazelrigg and Kaufman, 1983)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4594} NA P{KK100418} NA P{NIG.17603R} NA P{TRiP.GL00219} NA P{TRiP.HMS00416} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Taf1EP421 NA miscellaneous insertions PBac{RB}Taf1e02697 NA
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
histone serine kinase activity inferred from direct assay (Maile et al., 2004) contributes_to RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly inferred from direct assay (Metcalf and Wassarman, 2006, Wright et al., 2006, Verrijzer et al., 1995) sequence-specific DNA binding inferred from direct assay (Metcalf and Wassarman, 2006) TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from physical interaction with Tbp (Kokubo et al., 1994) transcription factor binding inferred from physical interaction with Taf11 (Yokomori et al., 1993) inferred from physical interaction with Taf12 (Yokomori et al., 1993)
Biological Process
CV term Evidence References
histone acetylation inferred from direct assay (Maile et al., 2004) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Wright et al., 2006) regulation of JAK-STAT cascade inferred from mutant phenotype (Kallio et al., 2010) regulation of transcription involved in G2/M-phase of mitotic cell cycle inferred from mutant phenotype (Maile et al., 2004)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Wright et al., 2006, Hansen and Tjian, 1995) transcription factor TFIID complex inferred from direct assay (Hansen and Tjian, 1995) inferred from physical interaction with Taf4 (Wright et al., 2006) inferred from physical interaction with Taf6 (Wright et al., 2006)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) protein kinase activity non-traceable author statement (Manning, 2002.11.16) zinc ion binding inferred from electronic annotation with InterPro:IPR001878 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein complex assembly non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) regulation of cell cycle non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) regulation of transcription, DNA-dependent non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human TAF1 AND inferred from sequence or structural similarity with Saccharomyces TAF1 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) transcription factor TFIID complex inferred from sequence or structural similarity with human TAF1 AND inferred from sequence or structural similarity with Saccharomyces TAF1 (Aoyagi and Wassarman, 2000) non-traceable author statement (Kokubo, 1993.7.21)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Taf1 - Stat92E anti bait coimmunoprecipitation, western blot (Panov et al., 2012) Taf1 - Taf10 anti bait coimmunoprecipitation, western blot (Georgieva et al., 2001) Taf1 - Tbp anti bait coimmunoprecipitation, western blot (Georgieva et al., 2001) Taf1 - e(y)2 anti bait coimmunoprecipitation, western blot (Georgieva et al., 2001)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Taf1 allele Gene References Taf1S-136 Ras85D (Karim et al., 1996) Ras85D::Src64B (Karim et al., 1996) Taf1S-625 dl (Pham and Sauer, 2000) sev (Maixner et al., 1998) Taf1XS-2232 dl (Pham and Sauer, 2000) Ras85D (Karim et al., 1996) Ras85D::Src64B (Karim et al., 1996) sev (Maixner et al., 1998) unspecified Ras85D (Sauer et al., 1996)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66011 DroID - A comprehensive database of gene and protein interactions. • FBgn0010355 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40813
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6R7VW0 Dipteran inclusive ortholog search EOG68PMT3 Insect inclusive ortholog search EOG62JMM7 Arthropod inclusive ortholog search EOG673N62 Metazoa inclusive ortholog search EOG6SV4X6
Orthologs in Drosophila Species (EOG6R7VW0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Taf1     Drosophila simulans   Dsim\GD19886 Y   Drosophila sechellia   Dsec\GM10907 Y   Drosophila erecta   Dere\GG13589 Y   Drosophila yakuba   Dyak\GE24876 Y   Drosophila ananassae   Dana\GF17182 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14575 Y   Drosophila persimilis   Dper\GL21765 Y   Drosophila willistoni   Dwil\GK12244 Y   Drosophila virilis   Dvir\Taf1 Y   Drosophila mojavensis   Dmoj\GI23838 Y   Drosophila grimshawi   Dgri\GH18122 Y
Orthologs in non-Drosophila Dipterans (EOG68PMT3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006082   Anopheles gambiae Malaria mosquito  Agam\AGAP003882   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003658
Orthologs in non-Dipteran Insects (EOG62JMM7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44341   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013825   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18150   Atta cephalotes Leafcutter ant  Acep\ACEP15930   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21885   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21157   Linepithema humile Argentine ant  Lhum\LH23087   Pogonomyrmex barbatus Red harvester ant  Pbar\PB10952   Solenopsis invicta Red fire ant  Sinv\SINV11061   Acyrthosiphon pisum Pea aphid  Apim\ACYPI56611   Bombyx mori Silkmoth  Bmor\BGIBMGA007052   Pediculus humanus Human body louse  Phum\PHUM537430   Tribolium castaneum Red flour beetle  Tcas\TC002507
Orthologs in non-Insect Arthropods (EOG673N62)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\LPCAT3   Ixodes scapularis Deer tick  Isca\ISCW012742
Orthologs in non-Arthropod Metazoa (EOG6SV4X6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\taf-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012482gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025624gn   Danio rerio Zebrafish  Drer\taf1   Xenopus tropicalis Western clawed frog  Xtro\taf1   Gallus gallus Domestic chicken  Ggal\TAF1   Mus musculus House mouse  Mmus\ENSMUSG00000031314   Rattus norvegicus Norway rat  Rnor\Taf1   Homo sapiens Human  Hsap\TAF1L   Homo sapiens Human  Hsap\TAF1
Human Orthologs (2)
Gene OMIM HGNC Hsap\TAF1L     Hsap\TAF1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19886 Drosophila sechellia Dsec\GM10907 Drosophila erecta Dere\GG13589 Drosophila yakuba Dyak\GE24876 Drosophila ananassae Dana\GF17182 Drosophila pseudoobscura pseudoobscura Dpse\GA14575 Drosophila persimilis Dper\GL21765 Drosophila willistoni Dwil\GK12244 Drosophila virilis Dvir\Taf1 Drosophila mojavensis Dmoj\GI23838 Drosophila grimshawi Dgri\GH18122
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	17281 w1118; P{EP}Taf1EP421/TM3, Sb1 5300 Taf11 red1 e1/TM3, Sb1 35314 y1 sc* v1; P{TRiP.GL00219}attP2 2066 y1; Taf1R14 red1 e1/TM2 32421 y1 sc* v1; P{TRiP.HMS00416}attP2
Harvard	- PBac{RB}Taf1e02697
Kyoto	108159 Taf11 red1 e1/TM3, Sb1 106647 y1; Taf1R14 red1 e1/TM2
VDRC	v106119 P{KK100418}VIE-260B v41099 w1118; P{GD4594}v41099/TM3
Genomic Clones ( 1 )
	BACR19I14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 84 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP15326 RE63802 RE74933
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03233 AT12414 GH17990 GH21239 GM01075 GM03821 LD02516 LD05618 LD06414 LD11315 LD21236 LD22676 LD28190 LD33825 LD40902 LD42310 LD42311 RE23390 RE64113
Other clones	CK01486 EC19960 EC33660 EK016001 EK020915 EK049501 EK115955 EK124723 EK140462 EK165828 EK173460 EK189318 EK202158 EK227842 EK235252 EK237405 EK252950 EK263225 EP16535 EP17928 IP15226 IP15426 IP15526 LD13531 LD40930 RC0-DA0006-130700-012-B06 RC0-DA0006-130700-012-B11 RC0-DA0006-130700-012-E03 RC0-DA0006-130700-012-E08 RC0-DA0006-130700-012-F06 RC0-DA0006-130700-012-F11 RC0-DA0006-130700-012-H01 RC0-DA0006-130700-021-C12 RC0-DA0006-130700-021-G11 RC0-DA0006-130700-021-H04 RP004137184 RP004145718 RP004156585 RP004175797 RP004180820 RP004199176 RP004215927 RP004224205 RP004226599 RP004233382 RP004236172 RP004282334 RP004310108 RP004362845 RP004385407 RP004422119 RP004433323 RP004463519 RP004472846 RP004482866 RP004483521 RP004494551 RP004500590 RP004524142 RP004533520 RP004556900 RP004558195
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010355 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40813
Antibody Information
	monoclonal (Weinzierl et al., 1993) polyclonal (Kokubo et al., 1993)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Taf250 cell (Wassarman, 1999.8.24) Source for merge of: Taf250 SR3-5 (Wassarman et al., 2000)
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) The proteins encoded by Taf1 and Taf4 have a specific role in transcription activation from TATA-less, downstream core promoter element (DPE)-containing promoters. (Wright et al., 2006) The proteins encoded by Tbp, Taf1, Taf2 and Taf11 are likely peripheral subunits of TFIID. (Wright et al., 2006) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, an increase in the proportion of G2/M phase cells, a decrease in mitotic index and a whole range of mitotic abnormalities are seen. (Bettencourt-Dias et al., 2004) The C-terminal domain of Taf1 phosphorylates His2B, but not His1, His2A, His3, or His4. The Taf1-mediated phosphorylation of His2B coincides with transcriptional activation of the stg and gt genes and therefore plays a part in mediating cell cycle progression. (Maile et al., 2004) Taf1 is a histone specific ubiquitin activating/conjugating enzyme (ubac). (Pham and Sauer, 2000) Taf1 is required in ovary, eye, ocelli, wing bristle and terminalia development and has a suggested role in regulating the cell cycle, cell differentiation, cell proliferation and cell survival. (Wassarman et al., 2000) Transcriptional regulation may involve, in part, competitive interactions between transcriptional activators and TAFs on the Tbp surface. (Nishikawa et al., 1997) Identified on the basis of genetic interaction with Ras85DV12.sev. (Karim et al., 1996) Taf1 exhibits histone acetyltransferase (HAT) activity in vitro. (Mizzen et al., 1996) Identification: Identified on the basis of genetic interaction with Ras85DV12.sev. (Sauer et al., 1996) Complementation group acts as a positive regulator of sev transcription. (Sauer et al., 1996) A quadruple complex containing Tbp, Taf1, Taf4 and Taf6 mediates transcriptional synergism by bcd and hb, whereas triple Tbp-Taf complexes lacking one or other coactivator failed to support synergistic activation. The concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during development. (Sauer et al., 1995) The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro. (Chen et al., 1994) TFIID subunit proteins and the Tbp protein can interact in pairwise combinations with several subunits in a network of interactions within TFIID. (Kokubo et al., 1994) N terminal sites of Taf1 mediate strong physical interaction with Tbp and inhibit Tbp function. Interaction of the N terminal region with Tbp may directly control TATA-box binding. (Kokubo et al., 1994) Taf1 contains at least two sites of interaction with Tbp. (Kokubo et al., 1994) Purified Taf5 is unable to bind Tbp directly or to interact strongly with the C-terminal domain of Taf1, but can only interact with a complex containing Tbp, Taf1, Taf4 and Taf6. (Dynlacht et al., 1993) In vitro expressed 'TAF110' interacts directly with the 'TAF250' which itself interacts with Tbp, a complex of all three is suggested. Binding study with various mutants of Taf1 suggests that both 'TAF110' and Tbp interact with the N-terminal 352-amino acid portion of 'TAF250'. (Kokubo et al., 1993) A number of polypeptides (encoded by Taf1, Taf4, Taf5, Taf6, e(y)1 and Taf12) that are tightly associated with Tbp and are native TFIID components have been purified. Protein blotting experiments suggest that one of these proteins, Taf1, interacts directly with Tbp, while the association between Tbp and the other proteins is either weak or is an indirect association via Taf1. (Kokubo et al., 1993) Taf1 gene product interacts directly with Tbp. (Kokubo et al., 1993) The assembly of a partial complex containing recombinant Tbf1, Taf1 and Taf4 was reported. This triple complex supports activation of HeLa cell Sp1 and reveals specific interactions between Tbp, Taf1 and Taf4. (Weinzierl et al., 1993) The Taf6 product interacts directly with Taf1 in vitro. (Weinzierl et al., 1993) Tbp, Taf1, Taf4 and Taf6 products can assemble into a mini-complex in vitro, showing that Taf6 and Taf4 occupy distinct binding sites on the C terminal part of Taf1. (Weinzierl et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057608 NM_206437 NM_206438 NM_001170061 NM_001260023 RefSeq (Proteins) • NP_476956 NP_996159 NP_996160 NP_001163532 NP_001246952 DDBJ / EMBL / GenBank • AAB26991 AAD19815 AAF54102 AAO47866 AAS65116 AAS65117 AC001652 ACZ94830 AE001572 AFH06271 AQ025084 BI580710 BT004888 CZ480469 S61883 Entrez Gene - A searchable database of RefSeq genes. • 40813 UniProtKB/Swiss-Prot • P51123 UniProtKB/TrEMBL • E1JJ72
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • AT hook, DNA-binding motif (IPR017956) Bromodomain, conserved site (IPR018359) Bromodomain (IPR001487) TAFII-230 TBP-binding (IPR009067) Zinc finger, CCHC-type (IPR001878) Transcription initiation factor TFIID subunit 1, animal (IPR011177) Transcription initiation factor TFIID subunit 1, domain of unknown function (IPR022591) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1TBA BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE63802
Linkouts
	BioGRID - A database of protein and genetic interactions • 66011 DroID - A comprehensive database of gene and protein interactions. • FBgn0010355 DRSC - Results from RNAi screens. • FBgn0010355 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010355 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17603-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010355 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40813 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /polycomb/tf2d2501.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40813 modMine - Data generated by the modENCODE project. • FBgn0010355
Synonyms & Secondary IDs ( 46 )
Reported As
Symbol Synonym	BG:DS00004.13   cel (Kaufman et al., 1990, Merrill et al., 1989, Wakimoto et al., 1984, ) cell (Mohler, 1997, ) CG17603 (Aoyagi and Wassarman, 2000, Metcalf and Wassarman, 2006, Keleman et al., 2009.8.5, Ni et al., 2010.12.1, Ni et al., 2011.7.19) d230 (Pugh, 2000) dmTAF1 (Lebedeva, 2005, Lebedeva et al., 2005, Muratoglu et al., 2003) dmTAFII230 (Pandey et al., 2002) dTAF230 (Wu et al., 2001) dTAF250 (Rabenstein et al., 1999) dTAFII230 (Georgieva et al., 2001, Pereira et al., 2001, Georgieva et al., 2000, Bagby et al., 2000, Weissman et al., 1998, Kotani et al., 1998, Burley and Roeder, 1998, Kuo and Allis, 1998, Liu et al., 1998, Tsukiyama and Wu, 1997, Mizzen et al., 1996, Nishikawa et al., 1997, Wright et al., 2006) dTAFII250 (Gangloff et al., 2001, Veenstra and Wolffe, 2001, Park et al., 2001, Pugh, 2000, Pham and Sauer, 2000, Albright and Tjian, 2000, Zhou et al., 1998, Lee and Young, 1998, Damania and Alwine, 1996, Goodrich and Tjian, 1994, Yokomori et al., 1993) dTAFII250 (Saurin et al., 2001) EfW1 (Hazelrigg and Kaufman, 1983) l(3)84Ab (Lindsley and Zimm, 1992, ) p230 (Kokubo et al., 1994, Kokubo et al., 1993, Kokubo et al., 1993, Kokubo et al., 1993) SR3-5 (Wassarman et al., 2000, Maixner et al., 1998, Sauer et al., 1996, Karim et al., 1996, ) TAF (Mueller and Tora, 2004) TAF1 (Tora, 2002, Maile et al., 2004, Maile et al., 2004, Marr et al., 2006, Wright et al., 2006, Klenov et al., 2007, Metcalf and Wassarman, 2006, Usakin et al., 2007, Katzenberger et al., 2006, Metcalf and Wassarman, 2007, Vorobyeva et al., 2009, Wright and Tjian, 2009, Marengo and Wassarman, 2008, Weake et al., 2009, Vorobyeva et al., 2011, Santoso and Kadonaga, 2006, Vorobyeva et al., 2009, Lebedeva et al., 2007, Soshnikova et al., 2008, Vorobyeva et al., 2008, Leser et al., 2012) Taf1 (Roy et al., 2007, Gan et al., 2010, Araripe et al., 2010, Xing et al., 2007, Vázquez et al., 2008, Christensen et al., 2009.2.28, Katzenberger et al., 2009, Veenstra, 2010, Anholt and Williams, 2010, Scheuermann et al., 2010, Kallio et al., 2010, Roy et al., 2011, Wasbrough et al., 2010, Neumüller et al., 2011, Japanese National Institute of Genetics, 2012.5.21) Taf1p (Kasahara et al., 2004) TAF200 (Hoey et al., 1993) Taf200   TAF230 (Kouzarides, 1999, Grunstein, 1997, Farmer et al., 1996) Taf230 (Richardson Rounds and Petri, 1996, ) TAF250 (Park et al., 2001, Martin, 1999.1.14, Kenyon et al., 2001, Aoyagi et al., 2000, Wassarman et al., 2000, Aoyagi and Wassarman, 1999, Hansen et al., 1997, Usakin et al., 2006) Taf250 (Wassarman, 1999.8.24, Bell and Tora, 1999, Struhl and Moqtaderi, 1998, Sauer et al., 1995, Verrijzer et al., 1995, Smith et al., 1993, Hori and Carey, 1994, Verrijzer et al., 1994, Kokubo et al., 1994, Weinzierl et al., 1993, ) TAF250/230 (Green, 2000, Aoyagi and Wassarman, 2000) TAFII (Arnosti, 2002) TAFII230 (Lebedeva, 2005, Lebedeva et al., 2005, Yang, 2004, Tora, 2002, Wassarman and Sauer, 2001, Leibovitch et al., 2002) TAFII250 (Conaway et al., 2002, Wassarman and Sauer, 2001, Mizzen and Allis, 2000, Pham et al., 1999, Naar et al., 1998, Schock et al., 1999, Aravind and Landsman, 1998, Cutler et al., 1998, Tsukiyama and Wu, 1997, Niessing et al., 1997, Jeanmougin et al., 1997, Sauer et al., 1996, Hansen and Tjian, 1995) TafII250 (Chen et al., 1994) TAFII250/230 (Shen et al., 1998, Tansey and Herr, 1997) TAFII250 (Kennett et al., 2002) TAFII250 (Wassarman et al., 2001, Dynlacht et al., 1993, Weinzierl et al., 1993, Schwartz and Pirrotta, 2007) TAFII-250 (Jones and Kadonaga, 2000) TFIID (Butler and Kadonaga, 2002, Simon and Tamkun, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001) TFIID TAF250 (Berk, 2000) unnamed (Lewis et al., 1980)
Name Synonym	cell lethal   Suppressor of Ras85D 3-5 (Karim et al., 1996) TATA-associated factor 250 (Weinzierl et al., 1993) TBP-associated factor (Schuettengruber et al., 2007) TBP-associated factor 1 (Roy et al., 2007, Xing et al., 2007) TBP-associated factor 250 (Dynlacht et al., 1993) TBP-associated factor 250kD   TFIID 230 kda subunit (Kokubo, 1993.7.21)
Secondary FlyBase IDs
	FBgn0004775 FBgn0010420 FBgn0016735
References ( 169 )
Generate a list of	All references relating to this gene ( 169 )
List References by type	Research paper  ( 95 ) Supplementary material  ( 8 ) Review  ( 33 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 8 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Spoof  ( 1 ) Letter  ( 2 ) Book  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 6 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Leser et al., 2012, Biol. Open 1(6): 597--606 The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility. [FBrf0220181] Panov et al., 2012, Nucleic Acids Res. 40(6): 2445--2453 Transcription co-activator SAYP mediates the action of STAT activator. [FBrf0218425] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123 Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912] Vorobyeva et al., 2011, Cell Cycle 10(11): 1821--1827 SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation. [FBrf0213948] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...