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Gene Dmel\Taf1

General Information
Symbol	Dmel\Taf1	Species	D. melanogaster
Name	TBP-associated factor 1	Annotation symbol	CG17603
Feature type	protein_coding_gene	FlyBase ID	FBgn0010355
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	TAF250, TAFII250, dTAFII230, dTAFII250, TAFII230, TFIID, TAFII250, SR3-5, TAF230, p230
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	84A1-84A1	Sequence location	3R:2,472,611..2,481,770 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TBP-associated factor 1 is referred to in FlyBase by the symbol Dmel\Taf1 (CG17603, FBgn0010355). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly; transcription factor binding; sequence-specific DNA binding; histone serine kinase activity; sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly. There is experimental evidence that it is involved in the biological process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription involved in G2/M-phase of mitotic cell cycle; regulation of JAK-STAT cascade; histone acetylation; neurogenesis. 30 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; sensillum; female germline cyst; external compound sense organ; adult mesothoracic segment; integumentary plate; gamete; peripheral nervous system. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: AT hook, DNA-binding motif; Bromodomain; Bromodomain, conserved site; TAFII-230 TBP-binding; Transcription initiation factor TFIID subunit 1, animal; Transcription initiation factor TFIID subunit 1, domain of unknown function; Zinc finger, CCHC-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1632256_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Taf1. Gene sequence location is 3R:2472611..2481770.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(3)84Ab Cell lethal; dies as normal-appearing second instar larva.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Controlled Vocabulary Terms TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly transcription factor binding sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly References Vorobyeva et al., 2009, Cell Cycle 8(14): 2152--2156 Yokomori et al., 1993, Genes Dev. 7(12B): 2587--2597 Sequence features BKN50947 BKN20514 HFA12607 BKN50819
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84A1-84A1   Limits computationally determined from genome sequence between P{lacW}casj1C2 and P{PZ}lab01241
Experimentally Determined Cytological Location
Cytogenetic map Notes References 84A2-84A3   (determined by in situ hybridisation)   (Weinzierl et al., 1993) 84A-84A   Deduced from meiotic mapping.   (Karim et al., 1996)
Experimentally Determined Recombination Data
Location	3- (Karim et al., 1996)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Taf1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Taf1-RA FBtr0081684  NM_057608   6552   2065 Taf1-RB FBtr0081685  NM_206438   6744   2129 Taf1-RC FBtr0081686  NM_206437   6651   2098 Taf1-RD FBtr0301622  NM_001170061   6645   2096 Taf1-RE FBtr0304903     6873   2172
Additional Transcript Data & Comments
Reported size (kB)	6.552 (compiled cDNA) (Kokubo et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Taf1-PA   FBpp0081184   232.2   2065   4.92     NP_476956   AAF54102 Taf1-PB   FBpp0089369   239.3   2129   4.92     NP_996160   AAS65116 Taf1-PC   FBpp0089420   236.0   2098   4.99     NP_996159   AAS65117 Taf1-PD   FBpp0290837   235.6   2096   4.86     NP_001163532   ACZ94830 Taf1-PE   FBpp0293442   244.1   2172   4.93
Additional Polypeptide Data & Comments
Reported size (kDa)	2068 (aa); 232 (kD predicted) (Kokubo et al., 1993) 250 (kD) (Weinzierl et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AT hook, DNA-binding motif (IPR017956) Bromodomain, conserved site (IPR018359) Bromodomain (IPR001487) TAFII-230 TBP-binding (IPR009067) Zinc finger, CCHC-type (IPR001878) Transcription initiation factor TFIID subunit 1, animal (IPR011177) Transcription initiation factor TFIID subunit 1, domain of unknown function (IPR022591) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1TBA
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141266   AA202346   AA246700   AA263181   AA392445   AA439081   AA567071   AA735391   AA802078   AA817407   AA949335   AA979324   AE014297 AAF54102   AE014297 AAS65116   AE014297 AAS65117   AC001652   AE014297 ACZ94830   AE001572 AAD19815   AI387456   AI514408   AI514425   AI516439   AI516440   AQ025084   BF495700   BF499008   BG633158   BG638043   BG638993   BI231764   BI481542   BI580710   BT004888 AAO47866   BT024196   BT025855   CO181997   CO186121   CO186553   CO190747   CO192409   CO264850   CO274929   CO277457   CO284340   CO293219   CO298675   CO306986   CO312079   CO314183   CO314665   CO315965   CO322966   CO343308   CO343733   CZ480469   EC057361   EC065391   EC066421   EC083209   EC197479   EC200658   EC201210   EC202847   EC203545   EC236975   EC244336   EC248902   EC249779   EC255001   EC260568   EL875201   EL875202   EL884060   EL884061   EL884173   ES690161   ES690162   ES690193   ES691077   ES692212   ES692216   ES692218   ES692221   ES692224   ES692226   GH945788   GH946209   GH948206   GH949916   GH950541   GH951616   GH952338   GH952905   GH959532   GH961761   GH962601   GH964586   GH965204   GH966106   GH966194   GH967052   GH967765   GH970018   GH970808   GH973231   GH974483   GH976805   GH977705   GH980982   GH981274   GH982839   GH984819   S61883 AAB26991
UniProtKB/Swiss-Prot	P51123
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0010355    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0010355 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0010355 expression level Developmental Stage   Expression Level embryo 00-02hr    3132 embryo 02-04hr    4738 embryo 04-06hr    3402 embryo 06-08hr    3221 embryo 08-10hr    2960 embryo 10-12hr    1954 embryo 12-14hr    1929 embryo 14-16hr    1964 embryo 16-18hr    1502 embryo 18-20hr    872 embryo 20-22hr    2143 embryo 22-24hr    1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr    1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day    1835 adult female 30day    1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3132) embryo 02-04hr  (4738) embryo 04-06hr  (3402) embryo 06-08hr  (3221) embryo 08-10hr  (2960) embryo 10-12hr  (1954) embryo 12-14hr  (1929) embryo 14-16hr  (1964) embryo 16-18hr  (1502) embryo 18-20hr  (872) embryo 20-22hr  (2143) embryo 22-24hr  (1776) larva L1  (885) larva L2  (733) larva L3 12hr old  (634) larva L3 puffstage 1-2  (614) larva L3 puffstage 3-6  (1064) larva L3 puffstage 7-9  (1132) white prepupae new  (1264) white prepupae 12hr  (1335) white prepupae 24hr  (1913) pupae 2d postWPP  (1190) pupae 3d postWPP  (628) pupae 4d postWPP  (471) adult male 01day  (726) adult male 05day  (941) adult male 30day  (886) adult female 01day  (1144) adult female 05day  (1835) adult female 30day  (1843) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3132) embryo 02-04hr  (4738) embryo 04-06hr  (3402) embryo 06-08hr  (3221) embryo 08-10hr  (2960) embryo 10-12hr  (1954) embryo 12-14hr  (1929) embryo 14-16hr  (1964) embryo 16-18hr  1502 embryo 18-20hr    872 embryo 20-22hr  (2143) embryo 22-24hr  (1776) larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr  (1913) pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day  (1835) adult female 30day  (1843) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3132 embryo 02-04hr    4738 embryo 04-06hr    3402 embryo 06-08hr    3221 embryo 08-10hr    2960 embryo 10-12hr    1954 embryo 12-14hr    1929 embryo 14-16hr    1964 embryo 16-18hr    1502 embryo 18-20hr    872 embryo 20-22hr    2143 embryo 22-24hr    1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr    1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day    1835 adult female 30day    1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3132 embryo 02-04hr    4738 embryo 04-06hr    3402 embryo 06-08hr    3221 embryo 08-10hr    2960 embryo 10-12hr    1954 embryo 12-14hr    1929 embryo 14-16hr    1964 embryo 16-18hr    1502 embryo 18-20hr    872 embryo 20-22hr    2143 embryo 22-24hr    1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr    1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day    1835 adult female 30day    1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0010355 expression level Developmental Stage   Expression Level embryo 00-02hr    3132 embryo 02-04hr    4738 embryo 04-06hr    3402 embryo 06-08hr    3221 embryo 08-10hr    2960 embryo 10-12hr    1954 embryo 12-14hr    1929 embryo 14-16hr    1964 embryo 16-18hr    1502 embryo 18-20hr    872 embryo 20-22hr    2143 embryo 22-24hr    1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr    1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day    1835 adult female 30day    1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3132) embryo 02-04hr  (4738) embryo 04-06hr  (3402) embryo 06-08hr  (3221) embryo 08-10hr  (2960) embryo 10-12hr  (1954) embryo 12-14hr  (1929) embryo 14-16hr  (1964) embryo 16-18hr  (1502) embryo 18-20hr  (872) embryo 20-22hr  (2143) embryo 22-24hr  (1776) larva L1  (885) larva L2  (733) larva L3 12hr old  (634) larva L3 puffstage 1-2  (614) larva L3 puffstage 3-6  (1064) larva L3 puffstage 7-9  (1132) white prepupae new  (1264) white prepupae 12hr  (1335) white prepupae 24hr  (1913) pupae 2d postWPP  (1190) pupae 3d postWPP  (628) pupae 4d postWPP  (471) adult male 01day  (726) adult male 05day  (941) adult male 30day  (886) adult female 01day  (1144) adult female 05day  (1835) adult female 30day  (1843) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3132) embryo 02-04hr  (4738) embryo 04-06hr  (3402) embryo 06-08hr  (3221) embryo 08-10hr  (2960) embryo 10-12hr  1954 embryo 12-14hr  1929 embryo 14-16hr  1964 embryo 16-18hr  1502 embryo 18-20hr    872 embryo 20-22hr  2143 embryo 22-24hr  1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr  1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day  1835 adult female 30day  1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3132 embryo 02-04hr    4738 embryo 04-06hr    3402 embryo 06-08hr    3221 embryo 08-10hr    2960 embryo 10-12hr    1954 embryo 12-14hr    1929 embryo 14-16hr    1964 embryo 16-18hr    1502 embryo 18-20hr    872 embryo 20-22hr    2143 embryo 22-24hr    1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr    1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day    1835 adult female 30day    1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3132 embryo 02-04hr    4738 embryo 04-06hr    3402 embryo 06-08hr    3221 embryo 08-10hr    2960 embryo 10-12hr    1954 embryo 12-14hr    1929 embryo 14-16hr    1964 embryo 16-18hr    1502 embryo 18-20hr    872 embryo 20-22hr    2143 embryo 22-24hr    1776 larva L1    885 larva L2    733 larva L3 12hr old    634 larva L3 puffstage 1-2    614 larva L3 puffstage 3-6    1064 larva L3 puffstage 7-9    1132 white prepupae new    1264 white prepupae 12hr    1335 white prepupae 24hr    1913 pupae 2d postWPP    1190 pupae 3d postWPP    628 pupae 4d postWPP    471 adult male 01day    726 adult male 05day    941 adult male 30day    886 adult female 01day    1144 adult female 05day    1835 adult female 30day    1843 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0010355    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0010355 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0010355 expression level Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0010355 expression level Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    272.65 Larval Midgut    71.3 Larval Hindgut    107.9 Larval Malpighian Tubules    107.4 Larval Fat Body    112.9 Larval Salivary Gland    164 Larval Trachea    146.275 Larval Carcass    91.775 Adult Head    97.2 Adult Eye    83.925 Adult Brain    188.8 Adult Thoracic-Abdominal Ganglion    149.8 Adult Crop    120.7 Adult Midgut    80.3 Adult Hindgut    84.6 Adult Malpighian Tubules    126.9 Adult Fat Body    114.8 Adult Salivary Gland    184.8 Adult Heart    93.35 Adult VirginFemale Spermatheca    191.1 Adult InseminatedFemale Spermatheca    174.3 Adult Ovary    229.6 Adult Testis    108.3 Adult Male Accessory Gland    171.4 Adult Carcass    98.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    97.2   NA  Eye    83.925   NA  Brain    188.8   272.65  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    149.8   NA  Crop    120.7   71.3  Midgut    80.3   107.9  Hindgut    84.6   107.4  Malpighian Tubules    126.9   112.9  Fat Body    114.8   164  Salivary Gland    184.8   NA  Heart    93.35   146.275  Trachea    NA   NA  VirginFemale Spermatheca    191.1   NA  InseminatedFemale Spermatheca    174.3   NA  Ovary    229.6   NA  Testis    108.3   NA  Male Accessory Gland    171.4   91.775  Carcass    98.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010355 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17603-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 Taf1GD4594 lethal | cell autonomous | larval stage | recessive Taf11 lethal | recessive Taf11 Taf12 Taf13 Taf14 Taf15 Taf16 Taf17 Taf18 Taf19 Taf110 Taf111 Taf112 Taf113 Taf114 Taf115 Taf1CTK Taf1R14 Taf1S-136 Taf1XS-2232 semi-lethal Taf1XS-2232 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Taf1GD4594 viable, with Scer\GAL4[-]: Taf1EP421 Sterility Allele female sterile Taf1S-136 Taf1S-625 Taf1XS-2232 Other Phenotypes Allele mitotic cell cycle defective Taf1dsRNA.cMa neuroanatomy defective | larval stage, with Scer\GAL41407 Taf1GD4594 visible, with Scer\GAL4pnr-MD237 Taf1GD4594 Phenotype manifest in Allele egg chamber Taf1S-625 egg chamber, with Scer\GAL4[-]: Taf1EP421 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 Taf1GD4594 eye photoreceptor cell Taf1XS-2232 eye photoreceptor cell, with Scer\GAL4[-]: Taf1EP421 ganglion mother cell | larval stage, with Scer\GAL41407 Taf1GD4594 interommatidial bristle Taf1XS-2232 interommatidial bristle, with Scer\GAL4[-]: Taf1EP421 macrochaeta Taf1S-136 Taf1S-625 Taf1XS-2232 macrochaeta, with Scer\GAL4[-]: Taf1EP421 male terminalia Taf1XS-2232 male terminalia, with Scer\GAL4[-]: Taf1EP421 mesothoracic tergum, with Scer\GAL4pnr-MD237 Taf1GD4594 microchaeta Taf1XS-2232 microchaeta, with Scer\GAL4[-]: Taf1EP421 ocellus Taf1XS-2232 ocellus, with Scer\GAL4[-]: Taf1EP421 oocyte Taf1XS-2232 oocyte, with Scer\GAL4[-]: Taf1EP421 ovary Taf1XS-2232 ovary, with Scer\GAL4[-]: Taf1EP421 second instar larva Taf11 wing Taf1XS-2232 wing, with Scer\GAL4[-]: Taf1EP421 wing vein L4 Taf1XS-2232 wing vein L4, with Scer\GAL4[-]: Taf1EP421
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of Taf1 Class Mutagen Stocks Known lesion Taf11 ethyl methanesulfonate 2 -- Taf1R14 ethyl methanesulfonate 2 -- Taf1e02697 piggyBac transposase 1 -- Taf1EP421 P-element activity 1 Yes Taf110 ethyl methanesulfonate 0 -- Taf111 ethyl methanesulfonate 0 -- Taf112 ethyl methanesulfonate 0 -- Taf113 ethyl methanesulfonate 0 -- Taf114 X ray 0 -- Taf115 ethyl methanesulfonate 0 -- Taf12 ethyl methanesulfonate 0 -- Taf13 X ray 0 -- Taf14 ethyl methanesulfonate 0 -- Taf15 ethyl methanesulfonate 0 -- Taf16 ethyl methanesulfonate 0 -- Taf17 ethyl methanesulfonate 0 -- Taf18 ethyl methanesulfonate 0 -- Taf19 ethyl methanesulfonate 0 -- Taf1CTK 0 Yes Taf1EP421-EX1 P-element activity 0 Yes Taf1S-136 ethyl methanesulfonate 0 Yes Taf1S-625 0 Yes Taf1XS-2232 X ray 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Taf1 Class Mutagen Stocks Known lesion Taf1GD4594 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1GL00219 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1HMS00416 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1KK100418 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf1Act5C.PF in vitro construct - regulatory fusion 0 Yes Taf1dsRNA.cMa in vitro construct - RNAi 0 Yes Taf1dsRNA.cWa in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Antp-X1 (Pham et al., 1999, Hazelrigg and Kaufman, 1983) Df(3R)Antp-X2 (Hazelrigg and Kaufman, 1983) Df(3R)BD5 (Wassarman et al., 2000) Df(3R)BSC738 (Christensen et al., 2009.2.28) Df(3R)Dfd13 (Hazelrigg and Kaufman, 1983) Df(3R)MAP2 (Merrill et al., 1989) Df(3R)Scr (Wakimoto et al., 1984, Merrill et al., 1989, Kaufman et al., 1990, Lewis et al., 1980) Df(3R)TplJE5 (Smoyer et al., 2003) T(2;3)VD3
Not disrupted in	Df(3R)Antp17 (Wakimoto et al., 1984, Kaufman et al., 1990, Lewis et al., 1980) Df(3R)Hu (Hazelrigg and Kaufman, 1983) Df(3R)MAP11 (Merrill et al., 1989) Df(3R)Scx2 (Hazelrigg and Kaufman, 1983, Wakimoto et al., 1984, Kaufman et al., 1990) Df(3R)Scx3 (Hazelrigg and Kaufman, 1983) Df(3R)Scx4 (Hazelrigg and Kaufman, 1983, Kaufman et al., 1990) Dp(3;3)Antp73b-rv8 (Hazelrigg and Kaufman, 1983) In(3R)Antprv9 (Hazelrigg and Kaufman, 1983) In(3R)ScrWrv5 (Hazelrigg and Kaufman, 1983) T(2;3)Antprv5 (Hazelrigg and Kaufman, 1983) T(2;3)Antprv6 (Hazelrigg and Kaufman, 1983) T(2;3)Antprv7 (Hazelrigg and Kaufman, 1983) T(2;3)ScrWrv1 (Hazelrigg and Kaufman, 1983) T(2;3)ScrWrv6 (Hazelrigg and Kaufman, 1983) Tp(3;3)Dfdrv1 (Hazelrigg and Kaufman, 1983) Tp(3;3)Dfdrv3 (Hazelrigg and Kaufman, 1983)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4594} NA P{KK100418} NA P{TRiP.GL00219} NA P{TRiP.HMS00416} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Taf1EP421 NA miscellaneous insertions PBac{RB}Taf1e02697 NA
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
histone serine kinase activity inferred from direct assay (Maile et al., 2004) contributes_to sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from direct assay (Metcalf and Wassarman, 2006, Wright et al., 2006, Verrijzer et al., 1995) sequence-specific DNA binding inferred from direct assay (Metcalf and Wassarman, 2006) TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from physical interaction with Tbp (Kokubo et al., 1994) transcription factor binding inferred from physical interaction with Taf11 (Yokomori et al., 1993) inferred from physical interaction with Taf12 (Yokomori et al., 1993)
Biological Process
CV term Evidence References
histone acetylation inferred from direct assay (Maile et al., 2004) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Wright et al., 2006) regulation of JAK-STAT cascade inferred from mutant phenotype (Kallio et al., 2010) regulation of transcription involved in G2/M-phase of mitotic cell cycle inferred from mutant phenotype (Maile et al., 2004)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Wright et al., 2006, Hansen and Tjian, 1995) transcription factor TFIID complex inferred from direct assay (Hansen and Tjian, 1995) inferred from physical interaction with Taf4 (Wright et al., 2006) inferred from physical interaction with Taf6 (Wright et al., 2006)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) protein kinase activity non-traceable author statement (Manning, 2002.11.16) zinc ion binding inferred from electronic annotation with InterPro:IPR001878 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein complex assembly non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) regulation of cell cycle non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) regulation of transcription, DNA-dependent non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human TAF1 AND inferred from sequence or structural similarity with Saccharomyces TAF1 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) transcription factor TFIID complex inferred from sequence or structural similarity with human TAF1 AND inferred from sequence or structural similarity with Saccharomyces TAF1 (Aoyagi and Wassarman, 2000) non-traceable author statement (Kokubo, 1993.7.21)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Taf1 allele Gene References Taf1S-136 Ras85D (Karim et al., 1996) Ras85D::Src64B (Karim et al., 1996) Taf1S-625 dl (Pham and Sauer, 2000) sev (Maixner et al., 1998) Taf1XS-2232 dl (Pham and Sauer, 2000) Ras85D (Karim et al., 1996) Ras85D::Src64B (Karim et al., 1996) sev (Maixner et al., 1998) unspecified Ras85D (Sauer et al., 1996)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66011 DroID - A comprehensive database of gene and protein interactions. • FBgn0010355 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40813
Orthologs
Genome-wide drosophilid orthologs	Dana\GF17182 Dere\GG13589 Dgri\GH18122 Dmoj\GI23838 Dper\GL21765 Dpse\GA14575 Dsec\GM10907 Dsim\GD19886 Dvir\Taf1 Dwil\GK12244 Dyak\GE24876
Curated drosophilid orthologs	Dana\Taf1 (Metcalf and Wassarman, 2006) Dere\Taf1 (Metcalf and Wassarman, 2006) Dmoj\Taf1 (Metcalf and Wassarman, 2006) Dpse\GA14575 (Metcalf and Wassarman, 2006, ) Dsim\Taf1 (Metcalf and Wassarman, 2006) Dvir\Taf1 (Metcalf and Wassarman, 2006) Dyak\Taf1 (Metcalf and Wassarman, 2006)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006082-PA Aedes aegypti (mosquito) AGAP003882-PA Anopheles gambiae (mosquito) XP_395639.3 Apis mellifera (honey bee) At1g32750.1 Arabidopsis thaliana (wall cress) 36239 Batrachochytrium dendrobatidis (chytrid fungus) 126682 Branchiostoma floridae (lancelet) BGIBMGA007052-PA Bombyx mori (silk worm) ENSBTAP00000009036 Bos taurus (bovine) CAL0006146 Candida albicans (a form of yeast) CN26039 Caenorhabditis brenneri (small nematode) CBP28269 Caenorhabditis briggsae (nematode) CE42634 Caenorhabditis elegans (worm) ENSCAFP00000025204 Canis familiaris (dog) CAGL0G00418g Candida glabrata (haploid yeast) ENSCINP00000003814 Ciona intestinalis (sea squirt) JA10752 Caenorhabditis japonica (nematode) CPIJ003658 Culex pipens (mosquito) RP33326 Caenorhabditis remanei (nematode) ENSCSAVP00000010606 Ciona savignyi (sedentary tunicate) 225982 Capitella spl (polychaete worm) DDB0220687 Dictyostelium discoideum (amoeba) 10162 Daphnia pulex (daphnia) ENSDARP00000051195 Danio rerio (zebra fish) ENSECAP00000005777 Equus caballus (horse) XP_657295 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000026935 Gymnopilus aculeatus (mushroom) ENSGALP00000008762 Gallus gallus (chicken) 194117 Hypsipyla robusta (cedar shoot borer) ENSP00000276072 Homo sapiens (human) ISCW012742-PA Ixodes scapularis (deer tick) KLLA0A10021g Kluyveromyces lactis (yeast) 212720 Lottia gigantea (owl limpet) ENSMODP00000005636 Monodelphis domestica (gray opossum) ENSMMUP00000007484 Macaca mulatta (rhesus monkey) ENSMUSP00000098895 Mus musculus (house mouse) ENSORLP00000010572 Oryzias latipes (japanese killifish) LOC_Os06g43790.1 Oryza sativa (asian rice) 34964 Ostreococcus tauri (green alga) PHUM003993-PA Pediculus humanus (human body louse) PP17354 Pristionchus pacificus (nematode) 94126 Physcomitrella patens (moss) ENSPPYP00000022899 Pongo pygmaeus (orangutan) 77562 Phytophthora ramorum (sudden oak death pathogen) 736035 Populus trichocarpa (black cottonwood) ENSPTRP00000059660 Pan troglodytes (chimpanzee) ENSRNOP00000003494 Rattus norvegicus (norway rat) 5061997 Sorghum bicolor (sorghum) YGR274C Saccharomyces cerevisiae (budding yeast) Smp_166840 Schistosoma mansoni (trematode parasite) SPAC2G11.14 Schizosaccharomyces pombe (fission yeast) XP_786789.2 Strongylocentrotus purpuratus (purple sea urchin) 56340 Trichoplax adhaerens (metazoa) XP_001811347.1 Tribolium castaneum (red flour beetle) ENSTNIP00000014742 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000039887 Takifugu rubripes (pufferfish Takifugu) 16.m00261 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000050061 Xenopus tropicalis (frog) YALI0F32153g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10952 NV50300 LH23087 ISCW012742 FBgn0241973 FBgn0214254 FBgn0260961 FBgn0191374 FBgn0165853 JGI_V11_10162 FBgn0074603 FBgn0159358 FBgn0146563 FBgn0125590 FBgn0105854 FBgn0094200 CPIJ003658 BGIBMGA007052 GB30349 GB11337 AGAP003882 AAEL006082 PHUM537430 GLEAN_02507
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	17281 w1118; P{EP}Taf1EP421/TM3, Sb1 5300 Taf11 red1 e1/TM3, Sb1 35314 y1 sc* v1; P{TRiP.GL00219}attP2 2066 y1; Taf1R14 red1 e1/TM2 32421 y1 sc* v1; P{TRiP.HMS00416}attP2
Harvard	- PBac{RB}Taf1e02697
Kyoto	108159 Taf11 red1 e1/TM3, Sb1 106647 y1; Taf1R14 red1 e1/TM2
VDRC	v106119 P{KK100418}VIE-260B v41099 w1118; P{GD4594}v41099/TM3
Genomic Clones ( 1 )
	BACR19I14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 84 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP15326 RE63802 RE74933
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03233 AT12414 GH17990 GH21239 GM01075 GM03821 LD02516 LD05618 LD06414 LD11315 LD21236 LD22676 LD28190 LD33825 LD40902 LD42310 LD42311 RE23390 RE64113
Other clones	CK01486 EC19960 EC33660 EK016001 EK020915 EK049501 EK115955 EK124723 EK140462 EK165828 EK173460 EK189318 EK202158 EK227842 EK235252 EK237405 EK252950 EK263225 EP16535 EP17928 IP15226 IP15426 IP15526 LD13531 LD40930 RC0-DA0006-130700-012-B06 RC0-DA0006-130700-012-B11 RC0-DA0006-130700-012-E03 RC0-DA0006-130700-012-E08 RC0-DA0006-130700-012-F06 RC0-DA0006-130700-012-F11 RC0-DA0006-130700-012-H01 RC0-DA0006-130700-021-C12 RC0-DA0006-130700-021-G11 RC0-DA0006-130700-021-H04 RP004137184 RP004145718 RP004156585 RP004175797 RP004180820 RP004199176 RP004215927 RP004224205 RP004226599 RP004233382 RP004236172 RP004282334 RP004310108 RP004362845 RP004385407 RP004422119 RP004433323 RP004463519 RP004472846 RP004482866 RP004483521 RP004494551 RP004500590 RP004524142 RP004533520 RP004556900 RP004558195
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010355 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40813
Antibody Information
	monoclonal (Weinzierl et al., 1993) polyclonal (Kokubo et al., 1993)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Taf250 cell (Wassarman, 1999.8.24) Source for merge of: Taf250 SR3-5 (Wassarman et al., 2000)
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) The proteins encoded by Taf1 and Taf4 have a specific role in transcription activation from TATA-less, downstream core promoter element (DPE)-containing promoters. (Wright et al., 2006) The proteins encoded by Tbp, Taf1, Taf2 and Taf11 are likely peripheral subunits of TFIID. (Wright et al., 2006) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, an increase in the proportion of G2/M phase cells, a decrease in mitotic index and a whole range of mitotic abnormalities are seen. (Bettencourt-Dias et al., 2004) The C-terminal domain of Taf1 phosphorylates His2B, but not His1, His2A, His3, or His4. The Taf1-mediated phosphorylation of His2B coincides with transcriptional activation of the stg and gt genes and therefore plays a part in mediating cell cycle progression. (Maile et al., 2004) Taf1 is a histone specific ubiquitin activating/conjugating enzyme (ubac). (Pham and Sauer, 2000) Taf1 is required in ovary, eye, ocelli, wing bristle and terminalia development and has a suggested role in regulating the cell cycle, cell differentiation, cell proliferation and cell survival. (Wassarman et al., 2000) Transcriptional regulation may involve, in part, competitive interactions between transcriptional activators and TAFs on the Tbp surface. (Nishikawa et al., 1997) Identified on the basis of genetic interaction with Ras85DV12.sev. (Karim et al., 1996) Taf1 exhibits histone acetyltransferase (HAT) activity in vitro. (Mizzen et al., 1996) Identification: Identified on the basis of genetic interaction with Ras85DV12.sev. (Sauer et al., 1996) Complementation group acts as a positive regulator of sev transcription. (Sauer et al., 1996) A quadruple complex containing Tbp, Taf1, Taf4 and Taf6 mediates transcriptional synergism by bcd and hb, whereas triple Tbp-Taf complexes lacking one or other coactivator failed to support synergistic activation. The concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during development. (Sauer et al., 1995) The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro. (Chen et al., 1994) TFIID subunit proteins and the Tbp protein can interact in pairwise combinations with several subunits in a network of interactions within TFIID. (Kokubo et al., 1994) N terminal sites of Taf1 mediate strong physical interaction with Tbp and inhibit Tbp function. Interaction of the N terminal region with Tbp may directly control TATA-box binding. (Kokubo et al., 1994) Taf1 contains at least two sites of interaction with Tbp. (Kokubo et al., 1994) Purified Taf5 is unable to bind Tbp directly or to interact strongly with the C-terminal domain of Taf1, but can only interact with a complex containing Tbp, Taf1, Taf4 and Taf6. (Dynlacht et al., 1993) In vitro expressed 'TAF110' interacts directly with the 'TAF250' which itself interacts with Tbp, a complex of all three is suggested. Binding study with various mutants of Taf1 suggests that both 'TAF110' and Tbp interact with the N-terminal 352-amino acid portion of 'TAF250'. (Kokubo et al., 1993) A number of polypeptides (encoded by Taf1, Taf4, Taf5, Taf6, e(y)1 and Taf12) that are tightly associated with Tbp and are native TFIID components have been purified. Protein blotting experiments suggest that one of these proteins, Taf1, interacts directly with Tbp, while the association between Tbp and the other proteins is either weak or is an indirect association via Taf1. (Kokubo et al., 1993) Taf1 gene product interacts directly with Tbp. (Kokubo et al., 1993) The assembly of a partial complex containing recombinant Tbf1, Taf1 and Taf4 was reported. This triple complex supports activation of HeLa cell Sp1 and reveals specific interactions between Tbp, Taf1 and Taf4. (Weinzierl et al., 1993) The Taf6 product interacts directly with Taf1 in vitro. (Weinzierl et al., 1993) Tbp, Taf1, Taf4 and Taf6 products can assemble into a mini-complex in vitro, showing that Taf6 and Taf4 occupy distinct binding sites on the C terminal part of Taf1. (Weinzierl et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057608 NM_206437 NM_206438 NM_001170061 RefSeq (Proteins) • NP_476956 NP_996159 NP_996160 NP_001163532 DDBJ / EMBL / GenBank • AAB26991 AAD19815 AAF54102 AAO47866 AAS65116 AAS65117 AC001652 ACZ94830 AE001572 AQ025084 BI580710 BT004888 CZ480469 S61883 Entrez Gene - A searchable database of RefSeq genes. • 40813 UniProtKB/Swiss-Prot • P51123
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • AT hook, DNA-binding motif (IPR017956) Bromodomain, conserved site (IPR018359) Bromodomain (IPR001487) TAFII-230 TBP-binding (IPR009067) Zinc finger, CCHC-type (IPR001878) Transcription initiation factor TFIID subunit 1, animal (IPR011177) Transcription initiation factor TFIID subunit 1, domain of unknown function (IPR022591) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1TBA
Linkouts
	BioGRID - A database of protein and genetic interactions • 66011 DroID - A comprehensive database of gene and protein interactions. • FBgn0010355 DRSC - Results from RNAi screens. • FBgn0010355 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010355 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17603-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010355 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40813 InParanoid A subset of ortholog calls from InParanoid. • AAEL006082-PA Aedes aegypti (mosquito) AGAP003882-PA Anopheles gambiae (mosquito) XP_395639.3 Apis mellifera (honey bee) At1g32750.1 Arabidopsis thaliana (wall cress) 36239 Batrachochytrium dendrobatidis (chytrid fungus) 126682 Branchiostoma floridae (lancelet) BGIBMGA007052-PA Bombyx mori (silk worm) ENSBTAP00000009036 Bos taurus (bovine) CAL0006146 Candida albicans (a form of yeast) CN26039 Caenorhabditis brenneri (small nematode) CBP28269 Caenorhabditis briggsae (nematode) CE42634 Caenorhabditis elegans (worm) ENSCAFP00000025204 Canis familiaris (dog) CAGL0G00418g Candida glabrata (haploid yeast) ENSCINP00000003814 Ciona intestinalis (sea squirt) JA10752 Caenorhabditis japonica (nematode) CPIJ003658 Culex pipens (mosquito) RP33326 Caenorhabditis remanei (nematode) ENSCSAVP00000010606 Ciona savignyi (sedentary tunicate) 225982 Capitella spl (polychaete worm) DDB0220687 Dictyostelium discoideum (amoeba) 10162 Daphnia pulex (daphnia) ENSDARP00000051195 Danio rerio (zebra fish) ENSECAP00000005777 Equus caballus (horse) XP_657295 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000026935 Gymnopilus aculeatus (mushroom) ENSGALP00000008762 Gallus gallus (chicken) 194117 Hypsipyla robusta (cedar shoot borer) ENSP00000276072 Homo sapiens (human) ISCW012742-PA Ixodes scapularis (deer tick) KLLA0A10021g Kluyveromyces lactis (yeast) 212720 Lottia gigantea (owl limpet) ENSMODP00000005636 Monodelphis domestica (gray opossum) ENSMMUP00000007484 Macaca mulatta (rhesus monkey) ENSMUSP00000098895 Mus musculus (house mouse) ENSORLP00000010572 Oryzias latipes (japanese killifish) LOC_Os06g43790.1 Oryza sativa (asian rice) 34964 Ostreococcus tauri (green alga) PHUM003993-PA Pediculus humanus (human body louse) PP17354 Pristionchus pacificus (nematode) 94126 Physcomitrella patens (moss) ENSPPYP00000022899 Pongo pygmaeus (orangutan) 77562 Phytophthora ramorum (sudden oak death pathogen) 736035 Populus trichocarpa (black cottonwood) ENSPTRP00000059660 Pan troglodytes (chimpanzee) ENSRNOP00000003494 Rattus norvegicus (norway rat) 5061997 Sorghum bicolor (sorghum) YGR274C Saccharomyces cerevisiae (budding yeast) Smp_166840 Schistosoma mansoni (trematode parasite) SPAC2G11.14 Schizosaccharomyces pombe (fission yeast) XP_786789.2 Strongylocentrotus purpuratus (purple sea urchin) 56340 Trichoplax adhaerens (metazoa) XP_001811347.1 Tribolium castaneum (red flour beetle) ENSTNIP00000014742 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000039887 Takifugu rubripes (pufferfish Takifugu) 16.m00261 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000050061 Xenopus tropicalis (frog) YALI0F32153g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /polycomb/tf2d2501.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40813 modMine - Data generated by the modENCODE project. • FBgn0010355 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10952 NV50300 LH23087 ISCW012742 FBgn0241973 FBgn0214254 FBgn0260961 FBgn0191374 FBgn0165853 JGI_V11_10162 FBgn0074603 FBgn0159358 FBgn0146563 FBgn0125590 FBgn0105854 FBgn0094200 CPIJ003658 BGIBMGA007052 GB30349 GB11337 AGAP003882 AAEL006082 PHUM537430 GLEAN_02507
Synonyms & Secondary IDs ( 46 )
Reported As
Symbol Synonym	BG:DS00004.13   cel (Kaufman et al., 1990, Merrill et al., 1989, Wakimoto et al., 1984, ) cell (Mohler, 1997, ) CG17603 (Aoyagi and Wassarman, 2000, Metcalf and Wassarman, 2006, Keleman et al., 2009.8.5, Ni et al., 2010.12.1, Ni et al., 2011.7.19) d230 (Pugh, 2000) dmTAF1 (Lebedeva, 2005, Lebedeva et al., 2005, Muratoglu et al., 2003) dmTAFII230 (Pandey et al., 2002) dTAF230 (Wu et al., 2001) dTAF250 (Rabenstein et al., 1999) dTAFII230 (Georgieva et al., 2001, Pereira et al., 2001, Georgieva et al., 2000, Bagby et al., 2000, Weissman et al., 1998, Kotani et al., 1998, Burley and Roeder, 1998, Kuo and Allis, 1998, Liu et al., 1998, Tsukiyama and Wu, 1997, Mizzen et al., 1996, Nishikawa et al., 1997, Wright et al., 2006) dTAFII250 (Gangloff et al., 2001, Veenstra and Wolffe, 2001, Park et al., 2001, Pugh, 2000, Pham and Sauer, 2000, Albright and Tjian, 2000, Zhou et al., 1998, Lee and Young, 1998, Damania and Alwine, 1996, Goodrich and Tjian, 1994, Yokomori et al., 1993) dTAFII250 (Saurin et al., 2001) EfW1 (Hazelrigg and Kaufman, 1983) l(3)84Ab (Lindsley and Zimm, 1992, ) p230 (Kokubo et al., 1994, Kokubo et al., 1993, Kokubo et al., 1993, Kokubo et al., 1993) SR3-5 (Wassarman et al., 2000, Maixner et al., 1998, Sauer et al., 1996, Karim et al., 1996, ) TAF (Mueller and Tora, 2004) TAF1 (Tora, 2002, Maile et al., 2004, Maile et al., 2004, Marr et al., 2006, Wright et al., 2006, Klenov et al., 2007, Metcalf and Wassarman, 2006, Usakin et al., 2007, Katzenberger et al., 2006, Metcalf and Wassarman, 2007, Wright and Tjian, 2009, Marengo and Wassarman, 2008, Weake et al., 2009, Vorobyeva et al., 2011, Santoso and Kadonaga, 2006, Vorobyeva et al., 2009) Taf1 (Roy et al., 2007, Gan et al., 2010, Xing et al., 2007, Vázquez et al., 2008, Christensen et al., 2009.2.28, Katzenberger et al., 2009, Veenstra, 2010, Anholt and Williams, 2010, Scheuermann et al., 2010, Kallio et al., 2010, Roy et al., 2011, Wasbrough et al., 2010, Neumüller et al., 2011) Taf1p (Kasahara et al., 2004) TAF200 (Hoey et al., 1993) Taf200   TAF230 (Kouzarides, 1999, Grunstein, 1997, Farmer et al., 1996) Taf230 (Richardson Rounds and Petri, 1996, ) TAF250 (Park et al., 2001, Martin, 1999.1.14, Kenyon et al., 2001, Aoyagi et al., 2000, Wassarman et al., 2000, Aoyagi and Wassarman, 1999, Hansen et al., 1997, Usakin et al., 2006) Taf250 (Wassarman, 1999.8.24, Bell and Tora, 1999, Struhl and Moqtaderi, 1998, Sauer et al., 1995, Verrijzer et al., 1995, Smith et al., 1993, Hori and Carey, 1994, Verrijzer et al., 1994, Kokubo et al., 1994, Weinzierl et al., 1993, ) TAF250/230 (Green, 2000, Aoyagi and Wassarman, 2000) TAFII (Arnosti, 2002) TAFII230 (Lebedeva, 2005, Lebedeva et al., 2005, Yang, 2004, Tora, 2002, Wassarman and Sauer, 2001, Leibovitch et al., 2002) TAFII250 (Conaway et al., 2002, Wassarman and Sauer, 2001, Mizzen and Allis, 2000, Pham et al., 1999, Naar et al., 1998, Schock et al., 1999, Aravind and Landsman, 1998, Cutler et al., 1998, Tsukiyama and Wu, 1997, Niessing et al., 1997, Jeanmougin et al., 1997, Sauer et al., 1996, Hansen and Tjian, 1995) TafII250 (Chen et al., 1994) TAFII250/230 (Shen et al., 1998, Tansey and Herr, 1997) TAFII250 (Kennett et al., 2002) TAFII250 (Wassarman et al., 2001, Dynlacht et al., 1993, Weinzierl et al., 1993, Schwartz and Pirrotta, 2007) TAFII-250 (Jones and Kadonaga, 2000) TFIID (Butler and Kadonaga, 2002, Simon and Tamkun, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001) TFIID TAF250 (Berk, 2000) unnamed (Lewis et al., 1980)
Name Synonym	cell lethal   Suppressor of Ras85D 3-5 (Karim et al., 1996) TATA-associated factor 250 (Weinzierl et al., 1993) TBP-associated factor (Schuettengruber, 2006) TBP-associated factor 1 (Roy et al., 2007, Xing et al., 2007) TBP-associated factor 250 (Dynlacht et al., 1993) TBP-associated factor 250kD   TFIID 230 kda subunit (Kokubo, 1993.7.21)
Secondary FlyBase IDs
	FBgn0004775 FBgn0010420 FBgn0016735
References ( 158 )
Generate a list of	All references relating to this gene ( 158 )
List References by type	Research paper  ( 87 ) Supplementary material  ( 7 ) Review  ( 33 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 8 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Spoof  ( 1 ) Letter  ( 2 ) Book  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 9 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123 Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912] Vorobyeva et al., 2011, Cell Cycle 10(11): 1821--1827 SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation. [FBrf0213948] Anholt and Williams, 2010, Chem. Senses 35(1): 21--30 The soluble proteome of the Drosophila antenna. [FBrf0209552] Barnes et al., 2010, BMC Biochem. 11: 26 Loss of the SIN3 transcriptional corepressor results in aberrant mitochondrial function. [FBrf0211360] Gan et al., 2010, Cell Res. 20(7): 763--783 Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq. [FBrf0211191] Kallio et al., 2010, FASEB J. 24(11): 4467--4479 Eye transformer is a negative regulator of Drosophila JAK/STAT signaling. [FBrf0212202] Scheuermann et al., 2010, Nature 465(7295): 243--247 Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB. [FBrf0210760] Veenstra, 2010, Insect Biochem. Mol. Biol. 40(4): 354--361 What the loss of the hormone neuroparsin in the melanogaster subgroup of Drosophila can tell us about its function. [FBrf0210499] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...