Gene Dmel\Akt1

(BDGP Project Members, 1994-1999)

89B6-89B7

89B-89C

(determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Akt1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

gene_with_non_canonical_start_codon ; SO:0001739

Unconventional translation start (ACG) postulated; FBrf0079853.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Akt1-RA

FBtr0083227

NM_169706

3728

530

Akt1-RB

FBtr0083226

NM_169707

3663

530

Akt1-RC

FBtr0083228

NM_169705

3663

611

Additional Transcript Data & Comments

Reported size (kB)

4.0, 2.8 (northern blot)

3.9, 2.7 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Akt1-PA

FBpp0082681

59.9

530

5.67

NP_732114

AAF55275

Akt1-PB

FBpp0082680

59.9

530

5.67

NP_732115

AAF55276

Akt1-PC

FBpp0082682

68.5

611

6.17

NP_732113

AAN13699

Additional Polypeptide Data & Comments

Reported size (kDa)

611, 530 (aa); 60 (kD predicted)

530 (aa)

Comments

Three different size Akt1 protein species of 66, 85, and 120kD are observed on western blots. The 66kD species is most prominent and is detected at all stages with the highest levels in embryos and pupae. The 85kD species is similarly expressed though at a lower level but is not detected in larvae. The 120kD species is detected mainly in early embryos and early pupae.

The 611aa form of Akt1 protein is a minor form apparently produced from translation from a weaker ACG upstream of the AUG.

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, catalytic domain (IPR000719)

AGC-kinase, C-terminal (IPR000961)

Pleckstrin homology domain (IPR001849)

Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290)

Serine/threonine-protein kinase, active site (IPR008271)

Protein kinase-like domain (IPR011009)

Pleckstrin homology-like domain (IPR011993)

Protein kinase, ATP binding site (IPR017441)

Protein kinase, C-terminal (IPR017892)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8INB9

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

embryonic stage 7 -- 8

organism | ubiquitous

posterior endoderm

trunk mesoderm

embryonic stage 9 -- 10

anterior endoderm

head mesoderm

organism | ubiquitous

posterior endoderm

trunk mesoderm

embryonic stage 11 -- 12

anterior midgut primordium

foregut primordium

hindgut proper primordium

organism | ubiquitous

posterior midgut primordium

somatic muscle primordium

Comment:reported as muscle system primordium

visceral muscle primordium

Comment:reported as muscle system primordium

trunk mesoderm

embryonic stage 13 -- 16

embryonic brain

embryonic foregut

embryonic/larval midgut

ventral nerve cord

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

embryonic stage | early

embryonic stage -- adult stage

pupal stage

adult stage | female

adult stage & oogenesis

nurse cell

Additional Descriptive Data

Akt1 transcripts are detected in embryo RNA and weakly in pupal RNA.

The 4kb Akt1 transcripts are detected throughout embryogenesis and larval stages on northern blots. They are also detected in pupal stages and in adult females. Akt1 transcripts are uniformly distributed at a high level in embryos and are observed in nurse cells during oogenesis.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval neuromuscular junction | restricted

(Guo and Zhong, 2006)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

embryonic stage -- adult stage

Additional Descriptive Data

Akt1 protein is detected in extracts from early embryos, adults, and S2 cells by Western blot. By immunolocalization, Akt1 protein is found to be uniformly distributed in embryos.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

cell surface

inferred from direct assay

cytoplasm

inferred from direct assay

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Expression Deduced from Reporters

Reporter: P{PZ}Akt1⁰⁴²²⁶

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

tracheal primordium

(Jin et al., 2001)

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		(756.1)
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		498.75
Larval Midgut		202.8
Larval Hindgut		282.9
Larval Malpighian Tubules		321
Larval Fat Body		431.5
Larval Salivary Gland		756.1
Larval Trachea		192.925
Larval Carcass		353.475
Adult Head		163.4
Adult Eye		118.775
Adult Brain		105.7
Adult Thoracic-Abdominal Ganglion		152.7
Adult Crop		362.5
Adult Midgut		195.8
Adult Hindgut		319.4
Adult Malpighian Tubules		41.2
Adult Fat Body		498.1
Adult Salivary Gland		212.4
Adult Heart		244.95
Adult VirginFemale Spermatheca		305.8
Adult InseminatedFemale Spermatheca		341.7
Adult Ovary		173.7
Adult Testis		36.5
Adult Male Accessory Gland		351.7
Adult Carcass		251.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	163.4
NA	Eye	118.775
NA	Brain	105.7
498.75	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	152.7
NA	Crop	362.5
202.8	Midgut	195.8
282.9	Hindgut	319.4
321	Malpighian Tubules	41.2
431.5	Fat Body	498.1
756.1	Salivary Gland	212.4
NA	Heart	244.95
192.925	Trachea	NA
NA	VirginFemale Spermatheca	305.8
NA	InseminatedFemale Spermatheca	341.7
NA	Ovary	173.7
NA	Testis	36.5
NA	Male Accessory Gland	351.7
353.475	Carcass	251.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		39
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		42
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		45
ovary, mated 4-day female		34
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(27)
central nervous system, larvae L3		(39)
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		(42)
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		(29)
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		(45)
ovary, mated 4-day female		(34)
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		39
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		42
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		45
ovary, mated 4-day female		34
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		39
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		42
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		45
ovary, mated 4-day female		34
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		39
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		42
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		45
ovary, mated 4-day female		34
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		(39)
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		(42)
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		(45)
ovary, mated 4-day female		(34)
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		39
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		42
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		45
ovary, mated 4-day female		34
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		27
central nervous system, larvae L3		39
central nervous system, pupae P8		17
head, virgin 1-day female		9
head, virgin 4-day female		9
head, virgin 20-day female		9
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		9
head, mated 1-day male		11
head, mated 4-day male		11
head, mated 20-day male		15
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		42
digestive system, larvae L3 wandering		22
digestive system, 1-day adult		10
digestive system, 4-day adult		11
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		26
fat body, pupae P8		12
carcass, larvae L3 wandering		29
carcass, 1-day adult		12
carcass, 4-day adult		13
carcass, 20-day adult		15
ovary, virgin 4-day female		45
ovary, mated 4-day female		34
testis, mated 4-day male		12
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		42
embryo 02-04hr		24
embryo 04-06hr		38
embryo 06-08hr		42
embryo 08-10hr		37
embryo 10-12hr		40
embryo 12-14hr		33
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		34
white prepupae new		35
white prepupae 12hr		37
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(42)
embryo 02-04hr		24
embryo 04-06hr		(38)
embryo 06-08hr		(42)
embryo 08-10hr		(37)
embryo 10-12hr		(40)
embryo 12-14hr		(33)
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		(34)
white prepupae new		(35)
white prepupae 12hr		(37)
white prepupae 24hr		(30)
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		(31)
adult female 30day		(33)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		42
embryo 02-04hr		24
embryo 04-06hr		38
embryo 06-08hr		42
embryo 08-10hr		37
embryo 10-12hr		40
embryo 12-14hr		33
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		34
white prepupae new		35
white prepupae 12hr		37
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		42
embryo 02-04hr		24
embryo 04-06hr		38
embryo 06-08hr		42
embryo 08-10hr		37
embryo 10-12hr		40
embryo 12-14hr		33
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		34
white prepupae new		35
white prepupae 12hr		37
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		33
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		42
embryo 02-04hr		24
embryo 04-06hr		38
embryo 06-08hr		42
embryo 08-10hr		37
embryo 10-12hr		40
embryo 12-14hr		33
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		34
white prepupae new		35
white prepupae 12hr		37
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(42)
embryo 02-04hr		24
embryo 04-06hr		(38)
embryo 06-08hr		(42)
embryo 08-10hr		(37)
embryo 10-12hr		(40)
embryo 12-14hr		(33)
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		(34)
white prepupae new		(35)
white prepupae 12hr		(37)
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		(33)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		42
embryo 02-04hr		24
embryo 04-06hr		38
embryo 06-08hr		42
embryo 08-10hr		37
embryo 10-12hr		40
embryo 12-14hr		33
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		34
white prepupae new		35
white prepupae 12hr		37
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		42
embryo 02-04hr		24
embryo 04-06hr		38
embryo 06-08hr		42
embryo 08-10hr		37
embryo 10-12hr		40
embryo 12-14hr		33
embryo 14-16hr		21
embryo 16-18hr		18
embryo 18-20hr		15
embryo 20-22hr		14
embryo 22-24hr		17
larva L1		12
larva L2		13
larva L3 12hr old		11
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		25
larva L3 puffstage 7-9		34
white prepupae new		35
white prepupae 12hr		37
white prepupae 24hr		30
pupae 2d postWPP		25
pupae 3d postWPP		16
pupae 4d postWPP		17
adult male 01day		15
adult male 05day		15
adult male 30day		15
adult female 01day		20
adult female 05day		31
adult female 30day		33
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		50
Schneider line 2 Sg4		23
embryonic 1182-4H		37
embryonic GM2		42
embryonic Kc167		31
embryonic S1		42
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		36
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(50)
Schneider line 2 Sg4		23
embryonic 1182-4H		(37)
embryonic GM2		(42)
embryonic Kc167		(31)
embryonic S1		(42)
embryonic S3		(31)
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		(29)
haltere disc ML-DmD17-c3		(32)
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		(36)
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		50
Schneider line 2 Sg4		23
embryonic 1182-4H		37
embryonic GM2		42
embryonic Kc167		31
embryonic S1		42
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		36
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		50
Schneider line 2 Sg4		23
embryonic 1182-4H		37
embryonic GM2		42
embryonic Kc167		31
embryonic S1		42
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		36
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		50
Schneider line 2 Sg4		23
embryonic 1182-4H		37
embryonic GM2		42
embryonic Kc167		31
embryonic S1		42
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		36
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(50)
Schneider line 2 Sg4		23
embryonic 1182-4H		(37)
embryonic GM2		(42)
embryonic Kc167		31
embryonic S1		(42)
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		(36)
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		50
Schneider line 2 Sg4		23
embryonic 1182-4H		37
embryonic GM2		42
embryonic Kc167		31
embryonic S1		42
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		36
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		50
Schneider line 2 Sg4		23
embryonic 1182-4H		37
embryonic GM2		42
embryonic Kc167		31
embryonic S1		42
embryonic S3		31
leg disc CME L1		17
wing disc CME-W2		19
wing disc ML-DmD8		21
wing disc ML-DmD9		18
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		21
wing disc ML-DmD32		29
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		15
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		36
ovary fGS/OSS		22
ovary OSC		24
ovary OSS		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		33
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		(31)
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		(33)
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		(29)
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(31)
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		33
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		33
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		33
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		(33)
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		33
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		16
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		33
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		26
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		9
Caffeine 1.5 mg/ml 4 hrs, larvae L3		11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		31
Caffeine 25 mg/ml 48 hrs, 4-day adult		14
Paraquat 5 mM 48 hrs, 4-day adult		26
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Show all FlyExpress processed images for Akt1

BDGP expression data

•

SD10374

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010379

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4006-RB

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

Akt1^GD1361

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

Akt1^GD1361

lethal, with Scer\GAL4^pnr-MD237

Akt1^GD1361

lethal | embryonic stage | germline clone | maternal effect

Akt1¹

lethal | embryonic stage | maternal effect

Akt1⁰⁴²²⁶

lethal | embryonic stage | maternal effect | recessive

Akt1¹

lethal | larval stage

Akt1¹

lethal | polyphasic | recessive

Akt1⁰⁴²²⁶

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Akt1^GD1361

lethal | recessive

partially lethal - majority die

Akt1⁰⁴²²⁶

partially lethal - majority die | recessive

Akt1⁰⁴²²⁶

viable

Sterility

Allele

female sterile | recessive

Akt1⁰⁴²²⁶

Other Phenotypes

Allele

body size defective (with Akt1¹)

Akt1⁰⁴²²⁶

body size defective (with Akt1⁰⁴²²⁶)

Akt1¹

body size defective | embryonic stage | recessive

Akt1¹

body size defective | pupal stage | recessive

Akt1¹

body size defective | recessive

Akt1¹

cell death defective | embryonic stage | maternal effect | recessive

Akt1¹

cell size defective | recessive | somatic clone

Akt1¹

chemical resistant, with Scer\GAL4^elav-C155

chemical sensitive, with Scer\GAL4^elav-C155

Akt1^{Scer\UAS.Exel}

circadian rhythm defective, with Scer\GAL4^Lk6-DJ634

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

circadian rhythm defective, with Scer\GAL4^tim.Scer\UAS

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

decreased cell death, with Scer\GAL4^hs.PB

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

decreased cell number, with Scer\GAL4^nub-AC-62

Akt1^GD1361

decreased cell size

Akt1⁰⁴²²⁶

decreased cell size, with Scer\GAL4^nub-AC-62

Akt1^GD1361

decreased cell size | somatic clone

Akt1¹

hyperplasia, with Scer\GAL4^GMR.PF

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

increased cell death | germline clone

Akt1¹

increased cell size, with Scer\GAL4^en-e16E

increased cell size, with Scer\GAL4^GMR.PF

Akt1^{Scer\UAS.T:Ivir\HA1}

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

Akt1^{Scer\UAS.T:Ivir\HA1}

long lived | RU486 conditional, with Scer\GAL4^bun-GSG5961

Akt1^GD1361

neuroanatomy defective

Akt1⁰⁴²²⁶

neuroanatomy defective, with Scer\GAL4^da.G32

Akt1^Scer\UAS.cRa

neuroanatomy defective | larval stage, with Scer\GAL4^ppk.PG

neuroanatomy defective | larval stage | somatic clone

Akt1^unspecified

neurophysiology defective | recessive | third instar larval stage

Akt1⁰⁴²²⁶

neurophysiology defective | third instar larval stage, with Akt1¹

Akt1^hs.PS

neurophysiology defective | third instar larval stage, with Akt1^hs.PS

Akt1¹

neurophysiology defective | third instar larval stage (with Akt1¹)

Akt1⁰⁴²²⁶

neurophysiology defective | third instar larval stage (with Akt1⁰⁴²²⁶)

Akt1¹

oxidative stress response defective, with Scer\GAL4^Lk6-DJ634

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

oxidative stress response defective, with Scer\GAL4^tim.Scer\UAS

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

planar polarity defective, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

short lived | heat sensitive, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B

Akt1^GD1361

size defective, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

size defective, with Scer\GAL4^GMR.PF

Akt1^{Scer\UAS.T:Ivir\HA1}

size defective, with Scer\GAL4^nub-AC-62

Akt1^GD1361

small body

small body | recessive

visible

Akt1^{T342D.S505D.GMR}

visible, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

visible, with Scer\GAL4^ap-md544

Akt1^{Scer\UAS.Exel}

visible, with Scer\GAL4^Bx-MS1096

Akt1^{Scer\UAS.Exel}

visible, with Scer\GAL4^dpp.blk1

Akt1^{Scer\UAS.T:Ivir\HA1}

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^GMR.PU

Akt1^{Scer\UAS.T:Ivir\HA1}

visible, with Scer\GAL4^hs.2sev

Akt1^{Scer\UAS.T:Ivir\HA1}

visible | recessive

Akt1¹

visible | recessive | somatic clone

Akt1¹

visible | somatic clone

Akt1¹

wild-type

Akt1^hs.PS

Phenotype manifest in

Allele

1st posterior cell, with Scer\GAL4^dpp.blk1

Akt1^{Scer\UAS.T:Ivir\HA1}

adult gut | heat sensitive, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B

Akt1^GD1361

adult gut | RU486 conditional, with Scer\GAL4^bun-GSG5961

Akt1^GD1361

dendrite | larval stage, with Scer\GAL4^ppk.PG

dendrite | larval stage | somatic clone

Akt1^unspecified

embryo

Akt1¹

embryonic/first instar larval cuticle | germline clone | maternal effect

Akt1¹

embryonic/first instar larval cuticle | maternal effect

Akt1⁰⁴²²⁶

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

Akt1^{Scer\UAS.T:Ivir\HA1}

embryonic/larval salivary gland, with Scer\GAL4^hs.PB

Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}

eye

eye, with Scer\GAL4^GMR.long

Akt1^{Scer\UAS.Exel}

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PU

Akt1^{Scer\UAS.T:Ivir\HA1}

eye, with Scer\GAL4^hs.2sev

Akt1^{Scer\UAS.T:Ivir\HA1}

eye | somatic clone

Akt1¹

head capsule | somatic clone

Akt1¹

interommatidial bristle, with Scer\GAL4^GMR.PF

Akt1^{Scer\UAS.T:Ivir\HA1}

neuromuscular junction | third instar larval stage, with Akt1¹

Akt1^hs.PS

neuromuscular junction | third instar larval stage, with Akt1^hs.PS

Akt1¹

neuromuscular junction | third instar larval stage (with Akt1¹)

Akt1⁰⁴²²⁶

neuromuscular junction | third instar larval stage (with Akt1⁰⁴²²⁶)

Akt1¹

ommatidium

Akt1^{T342D.S505D.GMR}

ommatidium, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^GMR.PU

Akt1^{Scer\UAS.T:Ivir\HA1}

ommatidium (with Akt1¹)

Akt1³

ommatidium (with Akt1³)

Akt1¹

ommatidium | somatic clone

Akt1¹

rhabdomere | somatic clone

Akt1¹

synapse

Akt1⁰⁴²²⁶

synapse | supernumerary, with Scer\GAL4^da.G32

Akt1^Scer\UAS.cRa

tracheal branch primordium

Akt1¹

tracheal branch primordium (with Df(3R)sbd45)

Akt1¹

wing, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

wing, with Scer\GAL4^Bx-MS1096

wing, with Scer\GAL4^dpp.blk1

Akt1^{Scer\UAS.T:Ivir\HA1}

wing, with Scer\GAL4^nub-AC-62

Akt1^GD1361

wing | posterior, with Scer\GAL4^en-e16E

Akt1^{Scer\UAS.Exel}

wing blade, with Scer\GAL4^ap-md544

Akt1^{Scer\UAS.Exel}

wing disc, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

wing disc | posterior compartment, with Scer\GAL4^en-e16E

Akt1^{Scer\UAS.T:Ivir\HA1}

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

Akt1^{Scer\UAS.T:Ivir\HA1}

wing hair, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

wing hair | somatic clone

Akt1¹

wing margin bristle | somatic clone, with Scer\GAL4^Act5C.PI

Akt1^{Scer\UAS.T:Ivir\HA1}

wing vein, with Scer\GAL4^71B

Akt1^{Scer\UAS.T:Ivir\HA1}

wing vein | ectopic (with Akt1¹)

Akt1³

wing vein | ectopic (with Akt1³)

Akt1¹

Classical Alleles ( 10 )

For All Classical Alleles Show

Allele of Akt1	Class	Mutagen	Stocks	Known lesion
Akt1⁰⁴²²⁶	hypomorphic allele - genetic evidence	P-element activity	1	Yes
Akt1^c02098		piggyBac transposase	1	--
Akt1^EY10012		P-element activity	1	--
Akt1¹	loss of function allele		0	Yes
Akt1²		ethyl methanesulfonate	0	--
Akt1³	hypomorphic allele - genetic evidence		0	Yes
Akt1^6M4			0	--
Akt1⁹			0	--
Akt1^EX4		P-element activity	0	Yes
Akt1^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 24 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Akt1	Mutagen	Stocks	Known lesion
Akt1^GMR.Exel	in vitro construct - regulatory fusion	2	Yes
Akt1^{Scer\UAS.Exel}	in vitro construct - regulatory fusion	2	Yes
Akt1^{T342D.S505D.GMR}	in vitro construct - amino acid replacement	2	Yes
Akt1^GD1361	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Akt1^HM04007	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Akt1^HMS00007	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Akt1^KK100495	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Akt1^{Act5C.T:Myr-Src64B}	in vitro construct - regulatory fusion	0	Yes
Akt1^{Act5C.T:Zzzz\FLAG}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Akt1^dsRNA.cPa	in vitro construct - RNAi	0	Yes
Akt1^dsRNA.cSa	in vitro construct - RNAi	0	Yes
Akt1^hs.PS	in vitro construct - regulatory fusion	0	Yes
Akt1^hs.PV	in vitro construct - regulatory fusion	0	Yes
Akt1^{hs.T:Uuuu\Myr4}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Akt1^K214M.hs	in vitro construct - regulatory fusion	0	Yes
Akt1^NIG.4006R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Akt1^R45A.tub	in vitro construct - amino acid replacement	0	Yes
Akt1^S505A.tub	in vitro construct - amino acid replacement	0	Yes
Akt1^Scer\UAS.cRa	in vitro construct - regulatory fusion	0	Yes
Akt1^Scer\UAS.cSa	in vitro construct - regulatory fusion	0	Yes
Akt1^{Scer\UAS.T:Ivir\HA1,T:Uuuu\MyrPalm1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Akt1^{Scer\UAS.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Akt1^T342A.tub	in vitro construct - amino acid replacement	0	Yes
Akt1^tub.PH	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel7328

(Ryder, 2004.4.26)

Df(3R)pnr^D4R+3

(Staveley et al., 1998, Heitzler et al., 1996)

Df(3R)sbd45

Df(3R)sbd105

(Spradling et al., 1999)

Tp(3;3)sbd¹⁰⁴

(BDGP Project Members, 1994-1999)

Not disrupted in

Df(3R)bxd100

Df(3R)E8-89B-Rev2

Df(3R)sbd26

Duplicated in

Dp(3;3)BK43

Dp(3;3)BK44

Not duplicated in

Ts(1Rt;3Rt)OR60+Ts(1Lt;3Lt)89Bq²

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

heat-shock construct

characterization construct

P{GMR-Akt1.Exel}

P{GMR-Akt1.T342D.S505D}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}Akt1^EY10012

miscellaneous insertions

PBac{PB}Akt1^c02098

insertion of enhancer trap

P{PZ}Akt1⁰⁴²²⁶

Gene Ontology: Function, Process & Cellular Component ( 32 unique terms )

Terms Based on Experimental Evidence ( 24 terms )

Molecular Function

CV term

References

protein kinase activity

inferred from direct assay

protein serine/threonine kinase activity

inferred from direct assay

(assigned by UniProtKB)

(Potter et al., 2002)

inferred from mutant phenotype

Biological Process

CV term

chitin-based embryonic cuticle biosynthetic process

References

inferred from genetic interaction with Pi3K21B

(Jin et al., 2001)

circadian rhythm

inferred from mutant phenotype

(Zheng et al., 2007)

dendrite regeneration

inferred from mutant phenotype

(Song et al., 2012)

epithelial cell migration, open tracheal system

inferred from mutant phenotype

(assigned by UniProtKB)

(Lavenburg et al., 2003)

insulin receptor signaling pathway

inferred from genetic interaction with UniProtKB:P91634

(assigned by UniProtKB)

intracellular signal transduction

inferred from genetic interaction with UniProtKB:P91634

(assigned by UniProtKB)

lipid storage

inferred from genetic interaction with Pten

long term synaptic depression

inferred from mutant phenotype

(Guo and Zhong, 2006)

negative regulation of apoptotic process

inferred from mutant phenotype

open tracheal system development

inferred from mutant phenotype

(Jin et al., 2001)

positive regulation of axon regeneration

inferred from mutant phenotype

(Song et al., 2012)

positive regulation of cell growth

inferred from mutant phenotype

(assigned by UniProtKB)

(Cavaliere et al., 2005, Scanga et al., 2000, Potter et al., 2002)

positive regulation of cell size

inferred from mutant phenotype

(Verdu et al., 1999, Scanga et al., 2000)

positive regulation of organ growth

inferred from mutant phenotype

protein phosphorylation

inferred from direct assay

(assigned by UniProtKB)

(Potter et al., 2002)

regulation of cell shape

inferred from mutant phenotype

regulation of dendrite development

inferred from mutant phenotype

(Parrish et al., 2009)

regulation of hemocyte proliferation

inferred from mutant phenotype

(Sinenko et al., 2010)

regulation of multicellular organism growth

inferred from mutant phenotype

response to oxidative stress

inferred from mutant phenotype

(Zheng et al., 2007)

Cellular Component

CV term

References

cell surface

inferred from direct assay

cytoplasm

inferred from direct assay

Terms Based on Predictions or Assertions ( 16 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR017441, InterPro:IPR017892

phosphatidylinositol binding

non-traceable author statement

(Oldham and Hafen, 2003)

phospholipid binding

inferred from electronic annotation with InterPro:IPR001849

(FlyBase Curators et al., 2004-)

protein serine/threonine kinase activity

inferred from sequence or structural similarity

(Franke, 1993.9.9, Hemmnings, 1994.12.14, Morrison et al., 2000)

non-traceable author statement

Biological Process

CV term

insulin receptor signaling pathway

References

non-traceable author statement

(Johnston and Gallant, 2002)

traceable author statement

open tracheal system development

traceable author statement

(Davies, 2002, Ghabrial et al., 2003)

positive regulation of cell growth

non-traceable author statement

(Johnston and Gallant, 2002)

traceable author statement

(Weinkove and Leevers, 2000, Oldham and Hafen, 2003)

positive regulation of cell size

non-traceable author statement

(Potter and Xu, 2001)

traceable author statement

(Oldham et al., 2000, Jacinto and Hall, 2003)

positive regulation of multicellular organism growth

traceable author statement

(Weinkove and Leevers, 2000, Oldham et al., 2000, Oldham and Hafen, 2003)

positive regulation of organ growth

non-traceable author statement

(Potter and Xu, 2001)

traceable author statement

(Oldham and Hafen, 2003, Hafen and Stocker, 2003)

protein phosphorylation

non-traceable author statement

(Morrison et al., 2000)

regulation of cell size

non-traceable author statement

regulation of multicellular organism growth

non-traceable author statement

regulation of organ growth

non-traceable author statement

(Weinkove and Leevers, 2000, Claeys et al., 2002)

regulation of protein import into nucleus

traceable author statement

(Ghabrial et al., 2003)

Cellular Component

CV term

References

plasma membrane

non-traceable author statement

(Oldham and Hafen, 2003)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Akt1 allele

Gene

References

Akt1¹

foxo

(Junger et al., 2003)

Pdk1

(Rintelen et al., 2001)

Pi3K92E

(Stocker et al., 2002, Lee et al., 2003)

(Stocker et al., 2002, Lee et al., 2003)

Akt1⁰⁴²²⁶

(Gao et al., 2000, Stocker et al., 2002)

Akt1^Scer\UAS.cRa

Pdk1

(Rintelen et al., 2001, Reiling and Hafen, 2004, Reiling et al., 2005)

Pi3K92E

S6k

Akt1^Scer\UAS.cSa

foxo

(Kramer et al., 2003)

Akt1^{Scer\UAS.T:Ivir\HA1}

foxo

(Puig et al., 2003)

gig

(Potter et al., 2002)

unspecified

(Goberdhan et al., 2005)

Thor

(Junger et al., 2003)

External Data

y¹ v¹; P{TRiP.HM04007}attP2

BioGRID - A database of protein and genetic interactions

•

67008

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0010379

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

41957

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

Q8INB9

Orthologs

OrthoDB Orthologs (66) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6868Z6

Dipteran inclusive ortholog search

EOG61ZFK6

Insect inclusive ortholog search

EOG60RZ9X

Arthropod inclusive ortholog search

EOG6M63ZS

Metazoa inclusive ortholog search

EOG6K9Q5P

Orthologs in Drosophila Species (EOG6868Z6)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17848

Drosophila persimilis

Dper\GL12220

Drosophila willistoni

Dwil\GK13262

Drosophila willistoni

Dwil\GK13260

Drosophila virilis

Dvir\GJ23383

Drosophila mojavensis

Dmoj\GI22655

Drosophila grimshawi

Dgri\GH18765

Orthologs in non-Drosophila Dipterans (EOG61ZFK6)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008823

Anopheles gambiae

Malaria mosquito

Agam\AGAP002161

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007754

Orthologs in non-Dipteran Insects (EOG60RZ9X)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\Akt1

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000313

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18967

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO21102

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL14658

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002231

Bombyx mori

Silkmoth

Bmor\BGIBMGA008304

Pediculus humanus

Human body louse

Phum\PHUM299490

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07749

Orthologs in non-Insect Arthropods (EOG6M63ZS)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\COI

Ixodes scapularis

Deer tick

Isca\ISCW018284

Orthologs in non-Arthropod Metazoa (EOG6K9Q5P)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\akt-1

Caenorhabditis elegans

Nematode

Cele\akt-2

Caenorhabditis elegans

Nematode

Cele\W10G6.2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_010805gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_017694gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007222gn

Danio rerio

Zebrafish

Drer\sgk1

Danio rerio

Zebrafish

Drer\sgk3

Danio rerio

Zebrafish

Drer\CU928197.1

Danio rerio

Zebrafish

Drer\OTTDARG00000013516

Danio rerio

Zebrafish

Drer\si:ch211-195b13.1

Danio rerio

Zebrafish

Drer\akt2l

Danio rerio

Zebrafish

Drer\akt3

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000033505

Xenopus tropicalis

Western clawed frog

Xtro\sgk1

Xenopus tropicalis

Western clawed frog

Xtro\akt1

Xenopus tropicalis

Western clawed frog

Xtro\sgk2

Xenopus tropicalis

Western clawed frog

Xtro\akt2

Gallus gallus

Domestic chicken

Ggal\SGK2

Gallus gallus

Domestic chicken

Ggal\AKT1

Gallus gallus

Domestic chicken

Ggal\SGK3

Gallus gallus

Domestic chicken

Ggal\AKT3

Gallus gallus

Domestic chicken

Ggal\SGK1

Mus musculus

House mouse

Mmus\Sgk3

Mus musculus

House mouse

Mmus\Akt2

Mus musculus

House mouse

Mmus\ENSMUSG00000019970

Mus musculus

House mouse

Mmus\Sgk2

Mus musculus

House mouse

Mmus\ENSMUSG00000019699

Rattus norvegicus

Norway rat

Rnor\Akt1

Rattus norvegicus

Norway rat

Rnor\Sgk1

Rattus norvegicus

Norway rat

Rnor\Sgk2

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000021497

Rattus norvegicus

Norway rat

Rnor\Akt2

Homo sapiens

Human

Hsap\AKT2

Homo sapiens

Human

Hsap\ENSG00000118515

Homo sapiens

Human

Hsap\SGK3

Homo sapiens

Human

Hsap\ENSG00000117020

Homo sapiens

Human

Hsap\AKT1

Homo sapiens

Human

Hsap\ENSG00000101049

Human Orthologs (6)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17848

Drosophila persimilis

Dper\GL12220

Drosophila willistoni

Dwil\GK13262

Drosophila virilis

Dvir\GJ23383

Drosophila mojavensis

Dmoj\GI22655

Drosophila grimshawi

Dgri\GH18765

Stocks & Reagents

Stocks Listed in FlyBase ( 13 )

Bloomington

8191

y¹ w¹¹¹⁸; P{UAS-Akt1.Exel}2

8192

P{UAS-Akt1.Exel}1, y¹ w¹¹¹⁸

8195

P{GMR-Akt1.Exel}1, y¹ w¹¹¹⁸

8196

y¹ w¹¹¹⁸; P{GMR-Akt1.Exel}2

31701

33615

y¹ v¹; P{TRiP.HMS00007}attP2

8193

P{GMR-Akt1.T342D.S505D}1, y¹ w¹¹¹⁸

8194

y¹ w¹¹¹⁸; P{GMR-Akt1.T342D.S505D}2

11627

ry⁵⁰⁶ P{PZ}Akt1⁰⁴²²⁶/TM3, ry^RK Sb¹ Ser¹

v103703

v2902

w¹¹¹⁸; P{GD1361}v2902

More stocks available...

Genomic Clones ( 1 )

BACR05C20

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 155 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

SD10374

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

(Andjelkovic et al., 1995, Lizcano et al., 2003, Kockel et al., 2010)

DRSC - Results from RNAi screens.

•

FBgn0010379

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

41957

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Akt1 CG4006

(Li, 1999.11)

Source for database merge of

Source for merge of: Akt1 l(3)04226

(Spradling et al., 1999)

Source for merge of: l(3)89Bs Akt1

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene results in a reduction in cell size.

(Guertin et al., 2006)

dsRNA made from templates generated with primers directed against this gene does not significantly inhibit S6k phosphorylation in S2 cells.

(Yang et al., 2006)

dsRNA made from templates generated with primers directed against this gene used to treat S2 cells.

(Yang et al., 2006)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become retracted (unspread but flat). Kc167 cells are unaffected.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

Data implicates Akt1 as a cell survival gene.

Mutants exhibit ectopic apoptosis during embryogenesis as judged by induction of membrane blebbing, DNA fragmentation and macrophage infiltration. Apoptosis caused by loss of Akt1 function is rescued by caspase suppression.

Btau\AKT1 is effective at rescuing Akt1 mutants, demonstrating Akt1 is as functional homolog of Btau\AKT1.

Complementation group identified during genetic analysis of the pnr region.

(Franke et al., 1994, Andjelkovic et al., 1995)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

(Perrimon et al., 1996)

Akt1 has been cloned and sequenced.

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

41957

UniProtKB/Swiss-Prot

•

Q8INB9

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, catalytic domain (IPR000719)

AGC-kinase, C-terminal (IPR000961)

Pleckstrin homology domain (IPR001849)

Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290)

Serine/threonine-protein kinase, active site (IPR008271)

Protein kinase-like domain (IPR011009)

Pleckstrin homology-like domain (IPR011993)

Protein kinase, ATP binding site (IPR017441)

Protein kinase, C-terminal (IPR017892)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

SD10374

Linkouts

BioGRID - A database of protein and genetic interactions

•

67008

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0010379

DRSC - Results from RNAi screens.

•

FBgn0010379

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010379

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4006-RB

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0010379

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

41957

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/dbzhnsky/akt1-1.htm

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

41957

modMine - Data generated by the modENCODE project.

•

FBgn0010379

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

Q8INB9

Synonyms & Secondary IDs ( 45 )

Reported As

Symbol Synonym

Akt

(Vereshchagina and Wilson, 2006, Shingleton, 2005, Bjorklund et al., 2006, Dong and Pan, 2004, Pan et al., 2004, Pagliarini et al., 2004, Sherr, 2004, Neufeld, 2003, Puig et al., 2003, Potter et al., 2002, Jin et al., 2001, Davies, 2002, Anselmo et al., 2002, Gao and Pan, 2001, Potter and Xu, 2001, Potter et al., 2001, Lu et al., 2001, Potter et al., 2000, Meier and Evan, 1998, Luo et al., 2006, Lavery and Stern, 2006, Dionne and Schneider, 2006, Zinke et al., 2007, Wilson et al., 2007, Dionne and Schneider, 2007, Yang et al., 2006, Takacs-Vellai, 2006, Wullschleger, 2006, Dionne et al., 2006, Gao et al., 2005, Fuss et al., 2007, Dionne, 2007, Hoshizaki and Gibbs, 2007, Libert, 2007, Ahrens et al., 2007, Fuss et al., 2006, Liu and Lehmann, 2006, Cavaliere et al., 2005, Dong et al., 2007, Berry and Baehrecke, 2007, Ikeya et al., 2009, Yu et al., 2008, Choi et al., 2008, Wang et al., 2008, Hennig et al., 2006, Lee and Chung, 2007, Lazzaro and Galac, 2006, Scanga et al., 2000, Haselton et al., 2010, Simcox et al., 2008, Alic et al., 2011, Mounir et al., 2011, Park et al., 2011, Yamaguchi et al., 2010, Sheldon et al., 2011, Biteau et al., 2010, Tang et al., 2011, Zhong et al., 2011, Lavery et al., 2007, Martin-Pena et al., 2006, Luo et al., 2007, Buttrick et al., 2008, Tseng et al., 2007, Lee et al., 2008, Nechipurenko and Broihier, 2012, Jovceva et al., 2007, Vereshchagina et al., 2008, Dutta and Baehrecke, 2008, Lee et al., 2008, Read et al., 2009, Shelly et al., 2009, Walkiewicz and Stern, 2009, Hyun et al., 2009, Ueishi et al., 2009, Schleich and Teleman, 2009, Qian and Bodmer, 2009, Branco et al., 2008, Parrish et al., 2009, Wu et al., 2009, Koike-Kumagai et al., 2009, Maynard et al., 2010, Tiefenböck et al., 2010, Puseenam et al., 2009, Sekine et al., 2010, Birse et al., 2010, Friedman et al., 2011, Gangaraju et al., 2011, Wang et al., 2008, Funakoshi et al., 2011, Chell and Brand, 2010, Chun-Jen Lin et al., 2011, Bjedov et al., 2010, Zheng et al., 2007, Bridon et al., 2012, Song et al., 2012, Patel and Hardy, 2012, Justiniano et al., 2012, Marshall et al., 2012, Callan et al., 2012, Wang et al., 2012)

akt

(Potter et al., 2001, Guo and Zhong, 2006, Parrish et al., 2009, Nechipurenko and Broihier, 2012)

Akt1

(Goberdhan et al., 2005, Ramel et al., 2007, Walker et al., 2006, Friedman and Perrimon, 2006, Schoenherr et al., 2012, Zhai et al., 2012, Sinenko et al., 2011, Dietzl et al., 2007, Bolukbasi et al., 2012, Sinenko et al., 2010, Zhou et al., 2008, Lindquist et al., 2011, Kaplan et al., 2008, Demontis and Perrimon, 2009, Harbison et al., 2009, Alvarez-Ponce et al., 2009, Nuzhdin et al., 2009, Tain et al., 2009, Cook et al., 2010.1.8, Pinal et al., 2006, Sims et al., 2009, Robinson et al., 2010, Fernández-Ayala et al., 2010, Aron et al., 2010, Curtis et al., 2007, Tsuda et al., 2010, Folberg-Blum et al., 2002, Terashima and Bownes, 2005, Eddison et al., 2011, Reis et al., 2010, Leventis et al., 2011, Tokusumi et al., 2012, Inamdar et al., 2012, Zheng et al., 2007, Murillo-Maldonado et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Kim et al., 2010, Sinenko et al., 2011, Noebels et al., 2012, Felix et al., 2012, Ye et al., 2012)

AKT1

(Kanao et al., 2012, Slack et al., 2010, Liévens et al., 2008, Kanao et al., 2010)

akt1

(Buttrick et al., 2008, Davidson et al., 2009, Coffman et al., 2005)

Akt1|PKB

(Alvarez-Ponce et al., 2012)

Akt/PKB

(Longo and Finch, 2003, Strauss, 2001, Siudeja et al., 2007)

AKT/PKB

(Gingras et al., 2001, Colombani et al., 2005)

CG4006

(Morrison et al., 2000, Whitfield, 2000.5.15, Demontis and Perrimon, 2009, Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2010.12.1)

Dakt

(Kohanski, 2002, Johnston and Gallant, 2002, Stocker and Hafen, 2000)

D-Akt

(Lavenburg et al., 2002, Scanga et al., 2000)

dakt

(Guo and Zhong, 2006, Kockel et al., 2010)

DAkt

(Orme et al., 2006)

dAKT

(Dekanty et al., 2005, Delanoue et al., 2010, Song et al., 2007, Kim et al., 2004)

dAkt1

(Inoki and Guan, 2006, Shi et al., 2005, Chen et al., 2003, Miron et al., 2001, Rintelen et al., 2001, Bradley et al., 2001, Coelho and Leevers, 2000, Cortina-Andrada et al., 2007, Igaki et al., 2006, Igaki et al., 2006)

Dakt1

(Potter et al., 2002, Jin et al., 2001, Downward and Leevers, 2001, Aigaki et al., 2002, Cho et al., 2001, Radimerski et al., 2001, Gao et al., 2000, Wetsch et al., 2000, Verdu et al., 1999, Edgar, 1999, Franke, 1993.9.9, Verdu et al., 1999, Staveley et al., 1998, Franke et al., 1994, Scanga et al., 2000, Lavenburg et al., 2003)

DAkt1

(Weinkove and Leevers, 2000)

DAKT1

(Leevers, 1999)

dakt1

(Nunez and del Peso, 1998)

dAKT1

(Moon et al., 2006)

DAKT1/PKB