Open Close
General Information
Symbol
Dmel\Akt1
Species
D. melanogaster
Name
Akt1
Annotation Symbol
CG4006
Feature Type
FlyBase ID
FBgn0010379
Gene Model Status
Stock Availability
Gene Snapshot
Akt1 (Akt1) encodes the core kinase component of the insulin/insulin-like growth factor pathway. It functions downstream of the product of Pi3K92E and is activated by phosphatidylinositol binding and phosphorylation. It regulates cell growth and survival, stress responses and ageing. [Date last reviewed: 2019-03-07]
Also Known As

Akt, dAkt, PKB, Dakt1, dPKB

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:16,099,228..16,105,670 [+]
Recombination map

3-58

RefSeq locus
NT_033777 REGION:16099228..16105670
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (41 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000683254
(assigned by GO_Central )
Biological Process (34 terms)
Terms Based on Experimental Evidence (33 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:P91634
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from genetic interaction with FLYBASE:Raf; FB:FBgn0003079
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000683254
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000683254
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. (Q8INB9)
Summaries
Gene Group (FlyBase)
AGC KINASES (UNCLASSIFIED) -
The AGC kinase subfamily are serine/threonine kinases that were originally defined based on the sequence similarity of the catalytic domain. The AGC KINASE (UNCLASSIFIED) gene group represents AGC kinases that do not fall into the PKA, PKC or PKG families. (Adapted from FBrf0132098 and PMID:20027184).
Pathway (FlyBase)
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
Pvr Signaling Pathway Core Components -
PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).
Negative Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Protein Function (UniProtKB)
Serine/threonine kinase involved in various developmental processes (PubMed:9601646, PubMed:10587646, PubMed:10962553, PubMed:11740943, PubMed:12172554, PubMed:11872800, PubMed:14525946, PubMed:12893776, PubMed:15466161, PubMed:15712201). During early embryogenesis, acts as a survival protein (PubMed:9601646, PubMed:10962553). During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination (PubMed:11740943). Also regulates tracheal cell migration (PubMed:11740943, PubMed:14525946). Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor (PubMed:10587646, PubMed:10962553, PubMed:11752451, PubMed:12893776, PubMed:25329475, PubMed:29025897, PubMed:24603715). Controls follicle cell size during oogenesis (PubMed:15712201). May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex (PubMed:12172554, PubMed:15466161). Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth (PubMed:10962553).
(UniProt, Q8INB9)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Akt1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

gene_with_non_canonical_start_codon ; SO:0001739

Unconventional translation start (ACG) postulated; FBrf0079853.

Gene model reviewed during 5.54

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0083227
3714
530
FBtr0083226
3649
530
FBtr0083228
3649
611
FBtr0344470
3301
530
FBtr0344717
4869
530
Additional Transcript Data and Comments
Reported size (kB)

3.9, 2.7 (northern blot)

4.0, 2.8 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0082681
59.9
530
5.67
FBpp0082680
59.9
530
5.67
FBpp0082682
68.5
611
6.17
FBpp0310841
59.9
530
5.67
FBpp0311050
59.9
530
5.67
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

530 aa isoforms: Akt1-PA, Akt1-PB, Akt1-PD, Akt1-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

611, 530 (aa); 60 (kD predicted)

Comments
External Data
Subunit Structure (UniProtKB)

Interacts with trbl.

(UniProt, Q8INB9)
Post Translational Modification

Phosphorylated and activated by Pk61C/PDK1 (PubMed:11344272). Phosphorylated on Ser-586 by the TORC2 complex (PubMed:10962553, PubMed:15718470, PubMed:22493059).

(UniProt, Q8INB9)
Domain

Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

(UniProt, Q8INB9)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Akt1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

The 4kb Akt1 transcripts are detected throughout embryogenesis and larval stages on northern blots. They are also detected in pupal stages and in adult females. Akt1 transcripts are uniformly distributed at a high level in embryos and are observed in nurse cells during oogenesis.

Akt1 transcripts are detected in embryo RNA and weakly in pupal RNA.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Expression Deduced from Reporters
Reporter: P{PZ}Akt104226
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Akt1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 38 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Akt1
Transgenic constructs containing regulatory region of Akt1
Deletions and Duplications ( 11 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (24)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
5  
12 of 15
No
Yes
10 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2  
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (22)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
3  
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (17)
12 of 13
Yes
Yes
11 of 13
No
Yes
11 of 13
No
Yes
3 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (49)
12 of 12
Yes
Yes
7 of 12
No
Yes
3 of 12
No
Yes
3 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (31)
10 of 15
Yes
Yes
10 of 15
Yes
Yes
10 of 15
Yes
Yes
7 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (13)
13 of 15
Yes
Yes
12 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (18)
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
No
1 of 9
Yes
Yes
1 of 9
Yes
No
1 of 9
Yes
Yes
1 of 9
Yes
Yes
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (5)
4 of 15
Yes
Yes
4 of 15
Yes
No
3 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
4 of 12
Yes
Yes
4 of 12
Yes
Yes
3 of 12
No
Yes
2 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905Y0 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503IE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05F3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05B6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06FF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (14)
4 of 10
4 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 5 )
Potential Models Based on Orthology ( 5 )
Modifiers Based on Experimental Evidence ( 9 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
suppressible
External Data
Subunit Structure (UniProtKB)
Interacts with trbl.
(UniProt, Q8INB9 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Insulin-like Receptor Signaling Pathway Core Components -
The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
Pvr Signaling Pathway Core Components -
PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).
Negative Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Metabolic Pathways
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map

3-58

Cytogenetic map
Sequence location
3R:16,099,228..16,105,670 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
89B3-89B3
Limits computationally determined from genome sequence between P{PZ}blp01618 and P{PZ}gish04895&P{EP}EP3171
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
89B6-89B7
(determined by in situ hybridisation)
89B4-89B10
(determined by in situ hybridisation)
89B-89C
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (22)
Genomic Clones (11)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (150)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Akt1 CG4006

    Source for database merge of

    Source for merge of: Akt1 l(3)04226

    Source for merge of: l(3)89Bs Akt1

    Additional comments
    Other Comments

    dsRNA made from templates generated with primers directed against this gene results in a reduction in cell size.

    dsRNA made from templates generated with primers directed against this gene used to treat S2 cells.

    dsRNA made from templates generated with primers directed against this gene does not significantly inhibit S6k phosphorylation in S2 cells.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become retracted (unspread but flat). Kc167 cells are unaffected.

    Btau\AKT1 is effective at rescuing Akt1 mutants, demonstrating Akt1 is as functional homolog of Btau\AKT1.

    Data implicates Akt1 as a cell survival gene.

    Mutants exhibit ectopic apoptosis during embryogenesis as judged by induction of membrane blebbing, DNA fragmentation and macrophage infiltration. Apoptosis caused by loss of Akt1 function is rescued by caspase suppression.

    Complementation group identified during genetic analysis of the pnr region.

    The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

    Akt1 has been cloned and sequenced.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 136 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (48)
    Reported As
    Symbol Synonym
    AKT
    (Venugopal et al., 2020, Chatterjee et al., 2019, Galenza and Foley, 2019, Hansen et al., 2019, Hwang et al., 2019, Jeong et al., 2019, Lang et al., 2019, Ryu et al., 2019, Kuo et al., 2018, Lee et al., 2018, Tang et al., 2018, Kang et al., 2017, Mattila and Hietakangas, 2017, Romero-Pozuelo et al., 2017, Sieber and Spradling, 2017, Tawo et al., 2017, Brill et al., 2016, Chen et al., 2016, Koyama and Mirth, 2016, Shen et al., 2016, Diop et al., 2015, Grifoni et al., 2015, Sanchez-Alvarez et al., 2015, Suh et al., 2015, Woodcock et al., 2015, Park et al., 2014, Wang et al., 2014, Andersen et al., 2013, Banerjee et al., 2013, Clark et al., 2013, Colca et al., 2013, Ferrandon, 2013, Gupta et al., 2013, Hada et al., 2013, Merkling and van Rij, 2013, Na et al., 2013, Nirala et al., 2013, Shim et al., 2013, Acebes et al., 2012, Homem and Knoblich, 2012, Lee et al., 2012, Murray et al., 2012, Wang et al., 2012, Harrison and Haddad, 2011, Resnik-Docampo and de Celis, 2011, Sun et al., 2011, Wang et al., 2011, Zhang et al., 2011, Ashton-Beaucage et al., 2010, Georgiev et al., 2010, Lee et al., 2010, Villa-Cuesta et al., 2010, Jones et al., 2009, Wu et al., 2009, Yan et al., 2009, Bakal et al., 2008, Kohyama-Koganeya et al., 2008, Liévens et al., 2008, Mattila et al., 2008, Hietakangas and Cohen, 2007, Hietakangas and Cohen, 2007, Schwartz and Pirrotta, 2007, Edgar, 2006, Liu and Lehmann, 2006, Leopold, 2004, Burgering and Kops, 2002, Morrison et al., 2000, Abrams, 1999, Spana and Perrimon, 1999)
    Akt
    (Lin and Hsu, 2020, Otsuki and Brand, 2020, Rambur et al., 2020, Thanh et al., 2020, Vega-Cuesta et al., 2020, Ahlers et al., 2019, Gil-Ranedo et al., 2019, Gupta et al., 2019, Johnson and Stolzing, 2019, Kim and Choi, 2019, Post et al., 2019, Ryu et al., 2019, Texada et al., 2019, Westfall et al., 2019, Yang et al., 2019, Bednářová et al., 2018, Das et al., 2018, Kang et al., 2018, Lee et al., 2018, Lehmann, 2018, Mattila et al., 2018, Palmer et al., 2018, Richardson and Portela, 2018, Roth et al., 2018, Spéder and Brand, 2018, Swevers et al., 2018, Villegas et al., 2018, Brace and DiAntonio, 2017, Jordán-Álvarez et al., 2017, Kim et al., 2017, Liu and Jin, 2017, Mathew et al., 2017, Murillo-Maldonado and Riesgo-Escovar, 2017, Warren et al., 2017, Wen et al., 2017, Wu et al., 2017, Yun et al., 2017, Alfa and Kim, 2016, Danielsen et al., 2016, David-Morrison et al., 2016, Hong et al., 2016, Jun et al., 2016, Niwa and Niwa, 2016, Padash Barmchi et al., 2016, Shen et al., 2016, Sieber et al., 2016, Vianna et al., 2016, Chen et al., 2015, Choo et al., 2015, Copf, 2015, Das and Dobens, 2015, Gross et al., 2015, Grotewiel and Bettinger, 2015, Huang et al., 2015, Jevtov et al., 2015, Kwon et al., 2015, Levayer et al., 2015, Nässel et al., 2015, Park et al., 2015, Rojas-Benitez et al., 2015, Song et al., 2015, Sopko et al., 2015, Ugrankar et al., 2015, Yan et al., 2015, Zaessinger et al., 2015, Alic et al., 2014, Banreti et al., 2014, Chuang et al., 2014, Ghosh et al., 2014, Gündner et al., 2014, Homem et al., 2014, Inamdar et al., 2014, Liu et al., 2014, Marzio et al., 2014, Moy et al., 2014, Mulakkal et al., 2014, Owusu-Ansah and Perrimon, 2014, Piccirillo et al., 2014, Schleich et al., 2014, Shimono et al., 2014, Wang et al., 2014, Xu and Cherry, 2014, Banerjee et al., 2013, Clark et al., 2013, Das et al., 2013, Das et al., 2013, Hirabayashi et al., 2013, Huang et al., 2013, Hwang et al., 2013, Kannan and Fridell, 2013, Karpac et al., 2013, Koyama et al., 2013, Lu and Johnston, 2013, Moy and Cherry, 2013, Na et al., 2013, Natarajan et al., 2013, O'Farrell et al., 2013, Oh et al., 2013, Pronovost et al., 2013, Shingleton and Frankino, 2013, Tamori and Deng, 2013, Tran et al., 2013, Wang et al., 2013, Wong et al., 2013, Yamanaka et al., 2013, Bier and Guichard, 2012, Bridon et al., 2012, Callan et al., 2012, Dahlgaard et al., 2012, Denton et al., 2012, Justiniano et al., 2012, Kapuria et al., 2012, Kuo et al., 2012, Lv et al., 2012, Marshall et al., 2012, Mirth and Shingleton, 2012, Nechipurenko and Broihier, 2012, Pallares-Cartes et al., 2012, Patel and Hardy, 2012, Rera et al., 2012, Song et al., 2012, Wang et al., 2012, Alic et al., 2011, Chun-Jen Lin et al., 2011, Friedman et al., 2011, Funakoshi et al., 2011, Gangaraju et al., 2011, Jones and Grotewiel, 2011, Miles et al., 2011, Mounir et al., 2011, Park et al., 2011, Partridge et al., 2011, Read, 2011, Sheldon et al., 2011, Tang et al., 2011, Willecke et al., 2011, Zhong et al., 2011, Birse et al., 2010, Biteau et al., 2010, Bjedov et al., 2010, Chell and Brand, 2010, Haselton et al., 2010, Maynard et al., 2010, Sabin et al., 2010, Sekine et al., 2010, Tiefenböck et al., 2010, Yamaguchi et al., 2010, Zheng and Sehgal, 2010, Hyun et al., 2009, Ikeya et al., 2009, Koike-Kumagai et al., 2009, Parrish et al., 2009, Puseenam et al., 2009, Qian and Bodmer, 2009, Read et al., 2009, Schleich and Teleman, 2009, Shelly et al., 2009, Ueishi et al., 2009, Walkiewicz and Stern, 2009, Wu et al., 2009, Branco et al., 2008, Buttrick et al., 2008, Choi et al., 2008, Dutta and Baehrecke, 2008, Lee et al., 2008, Lee et al., 2008, Simcox et al., 2008, Vereshchagina et al., 2008, Wang et al., 2008, Wang et al., 2008, Yu et al., 2008, Ahrens et al., 2007, Berry and Baehrecke, 2007, Dionne, 2007, Dionne and Schneider, 2007, Dong et al., 2007, Fuss et al., 2007, Hoshizaki and Gibbs, 2007, Jovceva et al., 2007, Lavery et al., 2007, Lee and Chung, 2007, Libert, 2007, Luo et al., 2007, Tseng et al., 2007, Wilson et al., 2007, Zheng et al., 2007, Zinke et al., 2007, Bjorklund et al., 2006, Dionne and Schneider, 2006, Dionne et al., 2006, Fuss et al., 2006, Hennig et al., 2006, Lavery and Stern, 2006, Lazzaro and Galac, 2006, Liu and Lehmann, 2006, Luo et al., 2006, Martin-Pena et al., 2006, Takacs-Vellai, 2006, Vereshchagina and Wilson, 2006, Wu and Brown, 2006, Wullschleger, 2006, Yang et al., 2006, Cavaliere et al., 2005, Gao et al., 2005, Shingleton, 2005, Dong and Pan, 2004, Pagliarini et al., 2004, Pan et al., 2004, Sherr, 2004, Neufeld, 2003, Puig et al., 2003, Anselmo et al., 2002, Davies, 2002, Potter et al., 2002, Gao and Pan, 2001, Jin et al., 2001, Lu et al., 2001, Potter and Xu, 2001, Potter et al., 2001, Potter et al., 2000, Scanga et al., 2000, Meier and Evan, 1998)
    Akt1
    (Zeng et al., 2020, Arbel et al., 2019, Chen and Read, 2019, Houtz et al., 2019, Li et al., 2019, Li et al., 2019, Manola et al., 2019, Meltzer et al., 2019, Xu et al., 2019, Yildirim et al., 2019, Ayala et al., 2018, Bai et al., 2018, Inoue et al., 2018, Merigliano et al., 2018, Sharma et al., 2018, Katti et al., 2017, Mensah et al., 2017, Neuert et al., 2017, Park et al., 2017, Stobdan et al., 2017, Transgenic RNAi Project members, 2017-, Bhadra et al., 2016, Cinnamon et al., 2016, Crocker et al., 2016, Dobson et al., 2016, Fischer et al., 2016, Kaynar et al., 2016, Kuleesha et al., 2016, Lin et al., 2016, Musashe et al., 2016, Slade and Staveley, 2016, Vinayagam et al., 2016, Fischer et al., 2015, Kwon et al., 2015, Li et al., 2015, Mensah et al., 2015, Siudeja et al., 2015, Slade and Staveley, 2015, Das et al., 2014, Lam et al., 2014, Liu et al., 2014, Nandi et al., 2014, Park et al., 2014, Sato-Miyata et al., 2014, Sopko et al., 2014, Tchankouo-Nguetcheu et al., 2014, Wei and Lilly, 2014, Das et al., 2013, Ibar et al., 2013, Kwon et al., 2013, Lanet et al., 2013, Lee et al., 2013, Lu and Johnston, 2013, Luo et al., 2013, Natarajan et al., 2013, Schertel et al., 2013, Sopko and Perrimon, 2013, Tixier et al., 2013, Tixier et al., 2013, Bolukbasi et al., 2012, Dahlgaard et al., 2012, Felix et al., 2012, Inamdar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Noebels et al., 2012, Rincon-Limas et al., 2012, Schoenherr et al., 2012, Tokusumi et al., 2012, Ye et al., 2012, Zhai et al., 2012, Eddison et al., 2011, Friedman et al., 2011, Leventis et al., 2011, Lindquist et al., 2011, Miles et al., 2011, Murillo-Maldonado et al., 2011, Sinenko et al., 2011, Sinenko et al., 2011, Aron et al., 2010, Cook et al., 2010.1.8, Fernández-Ayala et al., 2010, Kim et al., 2010, Kühnlein, 2010, Reis et al., 2010, Robinson et al., 2010, Sinenko et al., 2010, Tsuda et al., 2010, Alvarez-Ponce et al., 2009, Demontis and Perrimon, 2009, Harbison et al., 2009, Nuzhdin et al., 2009, Sims et al., 2009, Tain et al., 2009, Branco et al., 2008, Kaplan et al., 2008, Zhou et al., 2008, Chan et al., 2007, Curtis et al., 2007, Dietzl et al., 2007, Palomero et al., 2007, Ramel et al., 2007, Zheng et al., 2007, Friedman and Perrimon, 2006, Pinal et al., 2006, Walker et al., 2006, Goberdhan et al., 2005, Terashima and Bownes, 2005, Stanyon et al., 2004, Folberg-Blum et al., 2002, Pickeral et al., 2000)
    DRAC-PK66; DRAC-PK85
    RacPK
    Name Synonyms
    Protein kinase B
    RAC serine/threonine protein kinase
    Related to PKA to PKC protein kinases
    related to the A and C kinases
    Secondary FlyBase IDs
    • FBgn0010837
    • FBgn0013324
    • FBgn0017365
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (696)