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Gene Dmel\Def

General Information
Symbol	Dmel\Def	Species	D. melanogaster
Name	Defensin	Annotation symbol	CG1385
Feature type	protein_coding_gene	FlyBase ID	FBgn0010385
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	46D9-46D9	Sequence location	2R:5,941,683..5,942,081 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Defensin is referred to in FlyBase by the symbol Dmel\Def (CG1385, FBgn0010385). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: humoral immune response; defense response to Gram-positive bacterium; antibacterial humoral response. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Defensin, invertebrate/fungal; Knottin. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of no expression detected. Peak expression observed in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval trachea, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult midgut, adult salivary gland, adult ovary, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1634271_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Def. Gene sequence location is 2R:5941683..5942081.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Wong et al., 2011, PLoS ONE 6(9): e25430
FB2012_01	References Clark et al., 2011, Curr. Biol. 21(19): 1672--1677 Abdelsadik and Roeder, 2010, BMC Genomics 11: 265 Rao et al., 2011, Insect Biochem. Mol. Biol. 41(12): 982--992 Sequence features BKN31656 HFA07398
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46D9-46D9   Limits computationally determined from genome sequence between P{lacW}Adamk13906&P{EP}Pka-R2EP2162 and P{PZ}14-3-3ζ07103
Experimentally Determined Cytological Location
Cytogenetic map Notes References 46C-46D   (determined by in situ hybridisation)   (Dimarcq et al., 1994)
Experimentally Determined Recombination Data
Location	2-
Left of (cM)
Right of (cM)
Notes	Right arm.
Gene Model & Products
Please see the GBrowse view of Dmel\Def for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Def-RA FBtr0088432  NM_078948   399   92
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Def-PA   FBpp0087518   10.2   92   7.44     NP_523672   AAF58855
Additional Polypeptide Data & Comments
Reported size (kDa)	92 (aa) (Dimarcq et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Defensin, invertebrate/fungal (IPR001542) Knottin (IPR003614)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58855   AY224631 AAO72490   AY224632 AAO72491   AY224633 AAO72492   AY224634 AAO72493   AY224635 AAO72494   AY224636 AAO72495   AY224637 AAO72496   AY224638 AAO72497   AY224639 AAO72498   AY224640 AAO72499   AY224641 AAO72500   AY224642 AAO72501   BT023384   CA806837   CO284638   EL869773   Z27247 CAA81760
UniProtKB/Swiss-Prot	P36192
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval fat body   (Dimarcq et al., 1994) adult stage   adult fat body   (Dimarcq et al., 1994)
Additional Descriptive Data
	Def transcripts are detected in larvae and adults after infection with bacteria. They are detected at very low levels in pupae and adults in the absence of bacterial challenge. (Dimarcq et al., 1994)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0010385    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0010385 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of no expression detected. Peak expression observed in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0010385 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr    2589 white prepupae 24hr    2749 pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day    2743 adult female 01day    5161 adult female 05day    2775 adult female 30day    8747 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old  (115) larva L3 puffstage 1-2  (126) larva L3 puffstage 3-6    99 larva L3 puffstage 7-9  (148) white prepupae new  (1070) white prepupae 12hr  (2589) white prepupae 24hr  (2749) pupae 2d postWPP  (1002) pupae 3d postWPP  (117) pupae 4d postWPP  (163) adult male 01day  (1264) adult male 05day  (808) adult male 30day  (2743) adult female 01day  (5161) adult female 05day  (2775) adult female 30day  (8747) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr  (2589) white prepupae 24hr  (2749) pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day  (2743) adult female 01day  (5161) adult female 05day  (2775) adult female 30day  (8747) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr    2589 white prepupae 24hr    2749 pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day    2743 adult female 01day    5161 adult female 05day    2775 adult female 30day    8747 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr    2589 white prepupae 24hr    2749 pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day    2743 adult female 01day    5161 adult female 05day    2775 adult female 30day    8747 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0010385 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr    2589 white prepupae 24hr    2749 pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day    2743 adult female 01day    5161 adult female 05day    2775 adult female 30day    8747 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old  115 larva L3 puffstage 1-2  126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9  (148) white prepupae new  (1070) white prepupae 12hr  (2589) white prepupae 24hr  (2749) pupae 2d postWPP  (1002) pupae 3d postWPP  117 pupae 4d postWPP  (163) adult male 01day  (1264) adult male 05day  (808) adult male 30day  (2743) adult female 01day  (5161) adult female 05day  (2775) adult female 30day  (8747) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr  (2589) white prepupae 24hr  (2749) pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day  (2743) adult female 01day  (5161) adult female 05day  (2775) adult female 30day  (8747) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr    2589 white prepupae 24hr    2749 pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day    2743 adult female 01day    5161 adult female 05day    2775 adult female 30day    8747 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    6 embryo 04-06hr    38 embryo 06-08hr    17 embryo 08-10hr    12 embryo 10-12hr    24 embryo 12-14hr    10 embryo 14-16hr    5 embryo 16-18hr    8 embryo 18-20hr    11 embryo 20-22hr    12 embryo 22-24hr    10 larva L1    3 larva L2    5 larva L3 12hr old    115 larva L3 puffstage 1-2    126 larva L3 puffstage 3-6    99 larva L3 puffstage 7-9    148 white prepupae new    1070 white prepupae 12hr    2589 white prepupae 24hr    2749 pupae 2d postWPP    1002 pupae 3d postWPP    117 pupae 4d postWPP    163 adult male 01day    1264 adult male 05day    808 adult male 30day    2743 adult female 01day    5161 adult female 05day    2775 adult female 30day    8747 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0010385    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0010385 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval trachea, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult midgut, adult salivary gland, adult ovary, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0010385 expression level Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    2069.075 Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca    2360.5 Adult InseminatedFemale Spermatheca    1654.2 Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea  (2069.075) Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca  (2360.5) Adult InseminatedFemale Spermatheca  (1654.2) Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea  (2069.075) Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca  (2360.5) Adult InseminatedFemale Spermatheca  (1654.2) Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    2069.075 Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca    2360.5 Adult InseminatedFemale Spermatheca    1654.2 Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0010385 expression level Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    2069.075 Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca    2360.5 Adult InseminatedFemale Spermatheca    1654.2 Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea  (2069.075) Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca  (2360.5) Adult InseminatedFemale Spermatheca  (1654.2) Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea  (2069.075) Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca  (2360.5) Adult InseminatedFemale Spermatheca  1654.2 Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.925 Larval Midgut no informative data Larval Hindgut    53.2 Larval Malpighian Tubules    3.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    2069.075 Larval Carcass no informative data Adult Head    485.2 Adult Eye    28.775 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    6.2 Adult Crop no informative data Adult Midgut    165 Adult Hindgut no informative data Adult Malpighian Tubules    4 Adult Fat Body no informative data Adult Salivary Gland    105.1 Adult Heart no informative data Adult VirginFemale Spermatheca    2360.5 Adult InseminatedFemale Spermatheca    1654.2 Adult Ovary    132.8 Adult Testis    4.2 Adult Male Accessory Gland no informative data Adult Carcass    245.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    485.2   NA  Eye    28.775   NA  Brain    2.3   3.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.2   NA  Crop    no informative data   no informative data  Midgut    165   53.2  Hindgut    no informative data   3.2  Malpighian Tubules    4   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    105.1   NA  Heart    no informative data   2069.075  Trachea    NA   NA  VirginFemale Spermatheca    2360.5   NA  InseminatedFemale Spermatheca    1654.2   NA  Ovary    132.8   NA  Testis    4.2   NA  Male Accessory Gland    no informative data   no informative data  Carcass    245.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010385 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1385-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4da.G32 DefScer\UAS.cTa viable, with Scer\GAL4pnr-MD237 DefGD6789 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Def Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Def Class Mutagen Stocks Known lesion DefGD6789 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes DefHM05201 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes DefKK111656 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes DefScer\UAS.cTa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)pentA17 (Vuilleumier et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6789} NA P{KK111656} NA P{TRiP.HM05201} NA P{UAS-Def.T} NA reporter construct P{Def-lacZ.640} No
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
antibacterial humoral response inferred from expression pattern AND inferred from sequence or structural similarity (Dimarcq et al., 1994) defense response to Gram-positive bacterium inferred from direct assay (Levashina et al., 1995) inferred from expression pattern (Gordon et al., 2008) humoral immune response inferred from expression pattern (Verleyen et al., 2006)
Cellular Component
CV term Evidence References
extracellular space inferred from direct assay (Liu et al., 2006, Verleyen et al., 2006)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
defense response to bacterium non-traceable author statement (Kimbrell and Beutler, 2001) traceable author statement (Lemaitre et al., 1996) defense response to Gram-positive bacterium traceable author statement (Meister et al., 2000) positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria non-traceable author statement (Swiss-Prot Project Members, 1994.6.1)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Def allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010385 DroID - A comprehensive database of gene and protein interactions. • FBgn0010385
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12914 Dere\GG25236 Dgri\GH21581 Dmoj\GI19416 Dper\GL10559 Dpse\GA12570 Dsec\GM20557 Dsim\Def Dvir\GJ22479 Dwil\GK21361 Dyak\GE21830
Curated drosophilid orthologs	Dpse\GA12570   Dsec\Def (Dworkin and Jones, 2009) Dsim\Def
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL003841-PA Aedes aegypti (mosquito) AAEL003849-PA Aedes aegypti (mosquito) AAEL003857-PA Aedes aegypti (mosquito) NP_001011638.1 Apis mellifera (honey bee) CPIJ001276 Culex pipens (mosquito) XP_001603321.1 Nasonia vitripennis (wasp) XP_001605277.1 Nasonia vitripennis (wasp) PHUM011284-PA Pediculus humanus (human body louse) XP_967194.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV22776 NV14928 NV14926 NV14927 LH21592 FBgn0239073 FBgn0223353 FBgn0209590 FBgn0208259 FBgn0066167 FBgn0175439 FBgn0072616 FBgn0148169 FBgn0142290 FBgn0142154 FBgn0129042 FBgn0117360 FBgn0089948 CPIJ001276 GB10036 DEF1 DEFC_AEDAE PB20085 PB20087 PB20095 PB23767 PHUM595870 GLEAN_12469 GLEAN_10517 GLEAN_06250
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	29524 y1 v1; P{TRiP.HM05201}attP2
VDRC	v102437 P{KK111656}VIE-260B v38285 w1118; P{GD6789}v38285 v38286 w1118; P{GD6789}v38286
Genomic Clones ( 1 )
	BACR06J01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 4 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP01895
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	EK166450 ESG015b.E21_B03 IP01893
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010385 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36047
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Ecol\lacZDpt.PR adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism. (Lemaitre et al., 1997) Genes encoding antibacterial peptides are regulated in a manner distinct from that of Drs, encoding the antifungal peptide. (Lemaitre et al., 1996) Def has been cloned and sequenced, and its expression pattern has been analysed. (Dimarcq et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078948 RefSeq (Proteins) • NP_523672 DDBJ / EMBL / GenBank • AAF58855 AAO72490 AAO72491 AAO72492 AAO72493 AAO72494 AAO72495 AAO72496 AAO72497 AAO72498 AAO72499 AAO72500 AAO72501 AY224631 AY224632 AY224633 AY224634 AY224635 AY224636 AY224637 AY224638 AY224639 AY224640 AY224641 AY224642 CAA81760 Z27247 Entrez Gene - A searchable database of RefSeq genes. • 36047 UniProtKB/Swiss-Prot • P36192
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Defensin, invertebrate/fungal (IPR001542) Knottin (IPR003614)
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010385 DroID - A comprehensive database of gene and protein interactions. • FBgn0010385 DRSC - Results from RNAi screens. • FBgn0010385 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010385 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1385-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010385 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36047 InParanoid A subset of ortholog calls from InParanoid. • AAEL003841-PA Aedes aegypti (mosquito) AAEL003849-PA Aedes aegypti (mosquito) AAEL003857-PA Aedes aegypti (mosquito) NP_001011638.1 Apis mellifera (honey bee) CPIJ001276 Culex pipens (mosquito) XP_001603321.1 Nasonia vitripennis (wasp) XP_001605277.1 Nasonia vitripennis (wasp) PHUM011284-PA Pediculus humanus (human body louse) XP_967194.1 Tribolium castaneum (red flour beetle) modMine - Data generated by the modENCODE project. • FBgn0010385 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV22776 NV14928 NV14926 NV14927 LH21592 FBgn0239073 FBgn0223353 FBgn0209590 FBgn0208259 FBgn0066167 FBgn0175439 FBgn0072616 FBgn0148169 FBgn0142290 FBgn0142154 FBgn0129042 FBgn0117360 FBgn0089948 CPIJ001276 GB10036 DEF1 DEFC_AEDAE PB20085 PB20087 PB20095 PB23767 PHUM595870 GLEAN_12469 GLEAN_10517 GLEAN_06250 REDfly - A database of transcriptional regulatory elements. • FBgn0010385
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	143607_at (Kristensen et al., 2005) CG1385 (Irving et al., 2001, De Gregorio et al., 2001, Fulton et al., 2001, Liu et al., 2006, Winterhalter and Fedorka, 2009, Keleman et al., 2009.8.5, Verleyen et al., 2006, Perkins et al., 2009, Hernandez-Romano et al., 2008, Douglas et al., 2011) Def (Sackton and Clark, 2009, Shin, 2005, Chen et al., 2010, Cheng et al., 2011, Brun et al., 2006, Uvell and Engstrom, 2003, Blanco et al., 2010, Waterhouse et al., 2007, Lazzaro et al., 2004, Leulier et al., 2006, Ryu et al., 2008, Bergeret et al., 2008, Dijkers and O'Farrell, 2007, Buchon et al., 2009, Prokupek et al., 2009, Ren et al., 2007, Helenius et al., 2009, Hill-Burns and Clark, 2009, Clark et al., 2011, Galac and Lazzaro, 2011, Becker et al., 2010, Curtis et al., 2007, Chen et al., 2010, Gerbasi et al., 2011, Gordon et al., 2008) def (Leulier et al., 2000, Lee and Edery, 2008, Winterhalter and Fedorka, 2009, Marshall and Sinclair, 2010, Abdelsadik and Roeder, 2010) DEF (Christophides et al., 2002)
Name Synonym	defensin (Leclerc and Reichhart, 2004, Lemaitre, 2000.12.20, Hetru, 1993.11.11, Uttenweiler-Joseph et al., 1998, Craig et al., 2004, Malagoli et al., 2007, Ratnaparkhi and Courey, 2008, Christophides et al., 2002, Hallem et al., 2007, Lee and Edery, 2008, Lauwers et al., 2009, Winterhalter and Fedorka, 2009, Ooi et al., 2002, Kapelnikov et al., 2008, Marshall and Sinclair, 2010, Zsindely et al., 2009, Douglas et al., 2011) Defensin (Lazzaro et al., 2006, Cheng et al., 2011, Uvell and Engstrom, 2003, Matova and Anderson, 2010, Rutschmann et al., 2000, Matova and Anderson, 2006, Huh et al., 2007, Brennan et al., 2007, Lazzaro et al., 2004, Tanji et al., 2006, Wong et al., 2011, Leulier et al., 2006, Tanji et al., 2007, Ryu et al., 2008, Bergeret et al., 2008, Dijkers and O'Farrell, 2007, Wu et al., 2007, Lai et al., 2007, Ligoxygakis et al., 2002, Roxstrom-Lindquist et al., 2004, Charroux and Royet, 2009, Flatt et al., 2008, Luo et al., 2001, Verleyen et al., 2006, Carpenter et al., 2009, Helenius et al., 2009, Hill-Burns and Clark, 2009, Costa et al., 2009, Morozova et al., 2007, Clark et al., 2011, Beckstead et al., 2005, Fernández-Ayala et al., 2010, Becker et al., 2010, Vuilleumier et al., 2010, Radyuk et al., 2010, Wang et al., 2011, Akbar et al., 2011, Narbonne-Reveau et al., 2011, Gordon et al., 2008) Defensin precursor (Hernandez-Romano et al., 2008)
Secondary FlyBase IDs
References ( 132 )
Generate a list of	All references relating to this gene ( 132 )
List References by type	Research paper  ( 98 ) Supplementary material  ( 2 ) Review  ( 18 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 19 )
	Akbar et al., 2011, J. Cell Biol. 192(3): 383--390 The full-of-bacteria gene is required for phagosome maturation during immune defense in Drosophila. [FBrf0212933] Cheng et al., 2011, Int. J. Immunogenet. 38(4): 331--337 Coenzyme Q plays opposing roles on bacteria/fungi and viruses in Drosophila innate immunity. [FBrf0214057] Clark et al., 2011, Curr. Biol. 21(19): 1672--1677 Multiple TGF-β Superfamily Signals Modulate the Adult Drosophila Immune Response. [FBrf0216429] Douglas et al., 2011, Proc. Biol. Sci. 278(1704): 333--338 How the insect immune system interacts with an obligate symbiotic bacterium. [FBrf0212672] Galac and Lazzaro, 2011, Microbes Infect. 13(7): 673--683 Comparative pathology of bacteria in the genus Providencia to a natural host, Drosophila melanogaster. [FBrf0214338] Gerbasi et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(8): 3204--3209 Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis. [FBrf0213185] Narbonne-Reveau et al., 2011, PLoS ONE 6(2): e17470 Lack of an antibacterial response defect in Drosophila toll-9 mutant. [FBrf0213211] Rao et al., 2011, Insect Biochem. Mol. Biol. 41(12): 982--992 Manduca sexta moricin promoter elements can increase promoter activities of Drosophila melanogaster antimicrobial peptide genes. [FBrf0216595] Wang et al., 2011, Infect. Immun. 79(2): 606--616 Host and Pathogen Glycosaminoglycan-Binding Proteins Modulate Antimicrobial Peptide Responses in Drosophila melanogaster. [FBrf0212806] Wong et al., 2011, PLoS ONE 6(9): e25430 Wolbachia-mediated antibacterial protection and immune gene regulation in Drosophila. [FBrf0216316] Abdelsadik and Roeder, 2010, BMC Genomics 11: 265 Chronic activation of the epithelial immune system of the fruit fly's salivary glands has a negative effect on organismal growth and induces a peculiar set of target genes. [FBrf0214863] Becker et al., 2010, Nature 463(7279): 369--373 FOXO-dependent regulation of innate immune homeostasis. [FBrf0209804] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Chen et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(48): 20774--20779 Participation of the p38 pathway in Drosophila host defense against pathogenic bacteria and fungi. [FBrf0212390] Fernández-Ayala et al., 2010, PLoS ONE 5(1): e8549 Gene expression in a Drosophila model of mitochondrial disease. [FBrf0209705] Marshall and Sinclair, 2010, Proc. Biol. Sci. 277(1683): 963--969 Repeated stress exposure results in a survival-reproduction trade-off in Drosophila melanogaster. [FBrf0209895] Matova and Anderson, 2010, J. Cell Sci. 123(4): 627--633 Drosophila Rel proteins are central regulators of a robust, multi-organ immune network. [FBrf0209910] Radyuk et al., 2010, Biochim. biophys. Acta 1800(11): 1153--1163 Peroxiredoxin 5 modulates immune response in Drosophila. [FBrf0211669] Vuilleumier et al., 2010, Nat. Cell Biol. 12(6): 611--617 Control of Dpp morphogen signalling by a secreted feedback regulator. [FBrf0210884] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...