FlyBase Gene Report: Dmel\Acbp2

ACYL-COA BINDING PROTEINS -: The Acyl-CoA-binding proteins constitute a family of conserved proteins that bind long-chain acyl-Coenzyme A esters with high affinity. They are involved in multiple cellular tasks including intracellular acyl-CoA transport, modulation of fatty acid biosynthesis, vesicular trafficking and gene regulation. (Adapted from PMID:16018771.)

Protein Function (UniProtKB)

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters (By similarity). May be involved in energy metabolism in a manner that depends on the substrate used for energy production. Dbi and its metabolites are involved in the regulation of multiple biological processes.

(UniProt, P42281)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Acbp2 or the JBrowse view of Dmel\Acbp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0076915

420

FBtr0076916

386

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Acbp2-PA

FBpp0076624

9.6

8.90

NP_729218

AAF50607

Acbp2-PB

FBpp0076625

9.6

8.90

NP_523952

AAN12074

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

86 aa isoforms: Acbp2-PA, Acbp2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P42281

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acbp2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dbi_1

Linkouts

Gene Ontology (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

fatty-acyl-CoA binding

inferred from electronic annotation with InterPro:IPR000582

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Kolmer et al., 1994)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

cellular acyl-CoA homeostasis

inferred from sequence or structural similarity

(Kolmer et al., 1994)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

embryonic stage 11 -- 12

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Acbp2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Acbp2

View exonic expression by tissue for Dmel\Acbp2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010387

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010387

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8627-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8627

Flygut - An atlas of the Drosophila adult midgut

FBgn0010387

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Acbp2

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Acbp2

Transgenic constructs containing regulatory region of Acbp2

Deletions and Duplications ( 1 )

Disrupted in

Df(3L)Exel6110

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

Acbp2^GD13725

viable, with Scer\GAL4^pnr-MD237

Acbp2^GD13725

viable, with Scer\GAL4^tin.CΔ4

Acbp2^GD13725

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DBI

6 of 15

Yes

5 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Acbd7

5 of 15

Yes

Mmus\Dbi

5 of 15

Yes

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (7)

Rnor\Acbd7

4 of 13

Yes

Rnor\Dbi

4 of 13

Yes

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (6)

Xtro\acbd7

4 of 12

Yes

Xtro\acbd5

1 of 12

Xtro\acbd6

1 of 12

Xtro\dbi

1 of 12

Yes

Xtro\eci2

1 of 12

Xtro\mettl23

1 of 12

Danio rerio (Zebrafish) (8)

Drer\dbi

6 of 15

Yes

5 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\acbp-1

11 of 15

Yes

5 of 15

3 of 15

2 of 15

2 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\ACBP6

6 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ACB1

13 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC1539.06

11 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190JXC )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Acbp2

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21218

Drosophila persimilis

Dper\GL25029

Drosophila willistoni

Dwil\GK16653

Drosophila virilis

Dvir\GJ12061

Drosophila mojavensis

Dmoj\GI13295

Drosophila grimshawi

Dgri\GH14518

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150HQ7 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018341

Musca domestica

House fly

Mdoa\17021917

Glossina morsitans

Tsetse fly

Gmor\GMOY004439

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13495

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_08134

Mayetiola destructor

Hessian fly

Mdes\Mdes015313

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013844

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009214

Anopheles darlingi

American malaria mosquito

Adar\ADAC002291

Anopheles darlingi

American malaria mosquito

Adar\ADAC004239

Anopheles gambiae

Malaria mosquito

Agam\AGAP000546

Anopheles gambiae

Malaria mosquito

Agam\AGAP007460

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011388

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003820

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0NXF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002905

Bombyx mori

Silkmoth

Bmor\BGIBMGA002906

Danaus plexippus

Monarch butterfly

Dple\DPOGS212445-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS212442-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS212444-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL010952

Apis florea

Little honeybee

Aflo\2249421796

Apis mellifera

Western honey bee

Amel\LOC411272

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22842

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22575

Bombus terrestris

Buff-tailed bumblebee

Bter\15210477

Bombus terrestris

Buff-tailed bumblebee

Bter\15208089

Linepithema humile

Argentine ant

Lhum\LH26219

Linepithema humile

Argentine ant

Lhum\LH22017

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00090c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012109

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12961

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12960

Tribolium castaneum

Red flour beetle

Tcas\TC012627

Pediculus humanus

Human body louse

Phum\PHUM568230

Pediculus humanus

Human body louse

Phum\PHUM568220

Rhodnius prolixus

Kissing bug

Rpro\RPRC015244

Cimex lectularius

Bed bug

Clec\CLEC003105

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001471

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003355

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01385

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01386

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0PQ9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010019

Ixodes scapularis

Black-legged tick

Isca\ISCW002731

Stegodyphus mimosarum

African social velvet spider

Smim\KFM68430.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur22g01670

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_335945

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_307101

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230431

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_93842

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_308882

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_305152

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_41744

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0NXJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dbi

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Acbd6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_009843gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Acbd5

Ciona intestinalis

Vase tunicate

Cint\LOC100180079

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000021456

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000018492

Ciona intestinalis

Vase tunicate

Cint\LOC100184589

Gallus gallus

Domestic chicken

Ggal\METTL23

Gallus gallus

Domestic chicken

Ggal\ACBD6

Gallus gallus

Domestic chicken

Ggal\ACBD7

Gallus gallus

Domestic chicken

Ggal\ACBD5

Gallus gallus

Domestic chicken

Ggal\DBI

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

8 of 10

7 of 10

6 of 10

4 of 10

4 of 10

4 of 10

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DBI; diazepam binding inhibitor, acyl-CoA binding protein

6 of 15

125950

ACBD7; acyl-CoA binding domain containing 7

5 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0010387

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38784

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-19

Cytogenetic map

65E4-65E4

Sequence location

3L:7,131,863..7,133,463 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65E4-65E4

Limits computationally determined from genome sequence between P{lacW}l(3)L4060^L4060 and P{PZ}corn⁰⁴²⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65E-65E

(Roos, 1993.12.30)

65E3-65E9

(determined by in situ hybridisation)

(Kolmer et al., 1994)

Experimentally Determined Recombination Data

Location

3-19

(FlyBase, 2016)

3-16.6

(Comeron et al., 2012)

(Roos, 1993.12.30)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Bloomington

64850

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05723}attP40

VDRC

v23679

w¹¹¹⁸; P{GD13725}v23679

v23680

w¹¹¹⁸; P{GD13725}v23680

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

RH39533

Other clones

DPiM_RH39533

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010387

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010387

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38784

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Acbp2 Dbi

(Burton et al., 2005)

Source for database merge of

Source for merge of: Dbi BcDNA:RH39533

(FlyBase, 1996-)

Source for merge of: Dbi anon-WO0172774.131

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: Renamed from 'Dbi' because: (i) there's no evidence that the fly gene product is a "Diazepam-binding inhibitor"; (ii) one of the original authors that characterized the D. melanogaster gene (see FBrf0076972) later said that "the binding of Acyl-CoA is the only function of DBI/ACBP which has been supported with remarkable body of experimental evidence...it is highly unlikely that the real function of ACBP/DBI would be binding of diazepam" (FBrf0129015); and (iii) it makes sense to name this gene using the "Acbp" prefix as part of the "Acyl-CoA binding protein 1" family identified in the this paper.

(Burton et al., 2005)

Source for merge of Dbi BcDNA:RH39533 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of Dbi anon-WO0172774.131 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Isolated from a genomic library using a rat DBI cDNA as a probe, under low stringency conditions.

(Kolmer et al., 1994)

Dbi has been cloned and sequenced, and its expression pattern has been analysed.

(Kolmer et al., 1994)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38784

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information