FlyBase Gene Report: Dmel\Obp83b

General Information

Symbol

Dmel\Obp83b

Species

D. melanogaster

Name

Odorant-binding protein 83b

Annotation Symbol

CG11422

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010403

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

OS-E, 83b, Olfactory-specific E, DmelObp83b

Key Links

Genomic Location

Cytogenetic map

83D1-83D1

Sequence location

3R:5,976,177..5,976,817 [-]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:5976177..5976817

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables odorant binding

inferred from electronic annotation with InterPro:IPR006170, InterPro:IPR036728

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:lush; FB:FBgn0020277

(Vieira and Rozas, 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in sensory perception of chemical stimulus

inferred from sequence or structural similarity with FLYBASE:lush; FB:FBgn0020277

(Vieira and Rozas, 2011)

involved_in sensory perception of smell

inferred from biological aspect of ancestor with PANTHER:PTN000220814

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from sequence model

(Vieira and Rozas, 2011)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000220814

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ODORANT BINDING PROTEINS

Protein Family (UniProt)

Belongs to the PBP/GOBP family. (Q23970)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ODORANT BINDING PROTEINS -: Odorant Binding Proteins (OBP) are small (10 to 30 kDa) proteins secreted by auxiliary cells surrounding the olfactory receptor neurons. OBPs are characterized by a specific protein domain that comprises six α-helices joined by three disulfide bonds. Although the full function of the OBPs is not well established, it is believed that they may act as molecular carriers that transport odorants and deliver them to the olfactory receptors located on the sensory neurons. (Adapted from FBrf0205523).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Obp83b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q23970)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Obp83b-RA

FBtr0078655

NM_079518

528

141

Additional Transcript Data and Comments

Reported size (kB)

0.66 (northern blot)

(McKenna et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Obp83b-PA

FBpp0078304

16.1

141

6.01

Q23970

NP_524242

AAF51928

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

141 (aa); 16.1 (kD)

(McKenna et al., 1994)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q23970

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Obp83b using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Obp83b_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.51

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

trichoid sensillum of antennal segment 3

(McKenna et al., 1994)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

third segment of antenna

(McKenna et al., 1994)

antennal basiconic sensillum ab1

(Larter et al., 2016)

antennal basiconic sensillum ab2

(Larter et al., 2016)

antennal basiconic sensillum ab3

(Larter et al., 2016)

antennal basiconic sensillum ab7

(Larter et al., 2016)

trichoid sensillum of antennal segment 3

(Larter et al., 2016)

tormogen cell | subset of antennal segment 3 sense organ

(Larter et al., 2016)

Additional Descriptive Data

Expressed in antennal basiconic sensillum ab1-3 and 7 and in sensillum trichodeum. Observed in tormogen cells. Probably also present in trichogen cells but wasn't directly demonstrated.

(Larter et al., 2016)

Transcripts are detected in RNA from antennae but not in RNA from chemosensory depleted heads or bodies. In situ hybridization reveals that expression is limited to the ventro-lateral region of the third antennal segment, in a pattern consistent with the distribution of sensilla trichodea. In deeper sections, expression is observed beneath sensilla trichodea sensory hairs.

(McKenna et al., 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antennal trichoid sensillum at1

(Shanbhag et al., 2001)

antennal trichoid sensillum T2

(Shanbhag et al., 2001)

antennal trichoid sensillum T3

(Shanbhag et al., 2001)

antennal intermediate sensillum ai2

(Shanbhag et al., 2001)

antennal intermediate sensillum ai3

(Shanbhag et al., 2001)

tormogen cell | subset

(Shanbhag et al., 2001)

trichogen cell | subset

(Shanbhag et al., 2001)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Obp83b-lacZ.G}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antennal sense organ | subset

(Galindo and Smith, 2001)

chemosensory sensory organ of antenna

(Galindo and Smith, 2001)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Obp83b in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Obp83b

View exonic expression by tissue for Dmel\Obp83b

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40738/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010403

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010403

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010403

Flygut - An atlas of the Drosophila adult midgut

FBgn0010403

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Obp83b

Transgenic constructs containing regulatory region of Obp83b

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)Exel7284

(Ryder, 2004.4.26)

Df(3R)OS-EF-

(Scheuermann and Smith, 2019)

Gene partially disrupted in

Df(3R)Obp83-ab

(Xiao et al., 2019)

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Obp83b^f00970

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Obp83b^GD747

viable, with Scer\GAL4^pnr-MD237

Obp83b^GD747

Sterility

Allele

fertile

Obp83b^f00970

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (36)

Agam\OBP17

10 of 12

Yes

Agam\OBP2

5 of 12

Yes

Agam\OBP3

4 of 12

Yes

Agam\OBP15

4 of 12

Yes

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\OBP36

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\OBP45

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Obp83b. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

10 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0010403

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.5

Cytogenetic map

83D1-83D1

Sequence location

3R:5,976,177..5,976,817 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83D1-83D1

Limits computationally determined from genome sequence between P{lacW}cas^j1C2 and P{PZ}lab⁰¹²⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

83C-83D

(Hekmat-Scafe et al., 1995, McKenna, 1993.10.14)

83C1-83D5

(Hekmat-Scafe, 1996.12.9)

83C-83C

(determined by in situ hybridisation)

(Hekmatpanah et al., 1993)

83C-83D

(determined by in situ hybridisation)

(McKenna et al., 1994)

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

(Hekmat-Scafe et al., 1995)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

86050

w¹¹¹⁸; PBac{WH}Obp83b^f00970

44065

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02782}attP40

NIG-Fly

HMS02782

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02782}attP40/CyO

VDRC

v102252

P{KK111256}VIE-260B

v3405

w¹¹¹⁸ P{GD747}v3405

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP01759

Other clones

IP01760

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010403

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010403

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ha et al., 2023, Shanbhag et al., 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Shows alteration in expression in a Clk mutant background.

(McDonald and Rosbash, 2001)

Os-E and Pbprp3 are located about 1kb apart and contain a similar exon-intron organisation. Expression of both proteins is restricted spatially to two distinct types of olfactory hairs located in the ventrolateral region of the antenna. Immunocytochemical EM studies reveal that all sensilla examined express both proteins, thus, an individual sensilla can contain more than one odorant-binding protein.

(Hekmat-Scafe et al., 1997)

Isolated from a subtracted antennal cDNA library enriched for cDNAs expressed in the antennae but not the rest of the head or body of adult flies.

(McKenna et al., 1994)

An Os-E cDNA has been cloned and sequenced.

(McKenna et al., 1994)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Obp83b Os-E

(FlyBase, 2009-)

Nomenclature comments

'Os-E' renamed to 'Obp83b' to rationalize the nomenclature of 'Odorant-binding protein'-encoding genes consistent with the recommendations in FBrf0151704 and FBrf0205523.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

83b

(Graham and Davies, 2002, Smith, 2002.1.14, Galindo and Smith, 2001)

CG11422

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

DmelObp83b

(Vieira and Rozas, 2011, Sánchez-Gracia et al., 2009)

OBP83b

(Rihani et al., 2021, Rihani et al., 2019)

OS-E

(Scheuermann and Smith, 2019, Kong et al., 2010, Sánchez-Gracia and Rozas, 2008, Sanchez-Gracia and Rozas, 2007, Sanchez-Gracia et al., 2003, Graham and Davies, 2002, Hekmat-Scafe et al., 2000, Hekmat-Scafe et al., 1999, Yamamoto and Nakano, 1999, Hekmat-Scafe et al., 1997, Hekmat-Scafe, 1996.12.9, Hekmat-Scafe and Carlson, 1996, Hekmat-Scafe et al., 1995, McKenna et al., 1994, McKenna, 1993.10.14)

Obp83b

(Park et al., 2024, Maitra et al., 2019, Mohapatra and Menuz, 2019, Shultzaberger et al., 2019, Larter et al., 2016, Menuz et al., 2014, Librado and Rozas, 2013, Martin et al., 2013, Zhou et al., 2009, Sánchez-Gracia and Rozas, 2008, Vieira et al., 2007, Hekmat-Scafe, 2002.3.5, Hekmat-Scafe et al., 2002, Shanbhag et al., 2001)

Os-E

(Ha et al., 2023, Chatterjee et al., 2014, Immonen and Ritchie, 2012, Zhou et al., 2009)

PBP-H1

(Hekmatpanah et al., 1993)

obp83b

(de Bruyne and Warr, 2006)

Name Synonyms

Odorant-binding protein 83b

Olfactory-specific E

(Gao et al., 2021, Tsuda et al., 2006, Hekmat-Scafe et al., 1995)

Os-E

(Kong et al., 2010)

Pheromone-binding protein Homolog 1

(Hekmatpanah et al., 1993)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40738

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q23970

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q23970

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q23970

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40738/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010403

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010403

FlyMine - An integrated database for Drosophila genomics

FBgn0010403

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11422

MARRVEL_MODEL - MARRVEL (model organism gene)

40738

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010403

DroID - A comprehensive database of gene and protein interactions.

FBgn0010403

DRSC - Results frm RNAi screens

FBgn0010403

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Obp83b_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010403

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010403

Flygut - An atlas of the Drosophila adult midgut

FBgn0010403

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

References (70)

The following external sites may use different assemblies or annotations than FlyBase.

-0.51

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