Gene model reviewed during 5.39
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
152 (aa); 18 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS18 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\RpS18 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: RpS18 CG8900
"RpS18" likely corresponds to "M(2)56F".
Source for merge of RpS18 BcDNA:RH43343 was a shared cDNA ( date:030728 ).
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Likely Minute gene.
Deletions removing RpS18 but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype with a low cell count when assayed in Kc167 cells.
A clone (λDmS18) containing a ribosomal protein gene that may encode an RPS18 protein has been identified. The clone hybridises in situ to 15B.
This may correspond to M(2)56F (FBrf0073138). Burns et al. (FBrf0041468) identified a clone that hybrid-selected an RNA that translated to give a protein identified as RPS18. The clone (λDmS18) hybridized in situ to 15B and these authors concluded that "M(1)o" (i.e. RpS5a) encoded this protein. McKim and Hawley ( EMBL:U48394 ) have shown, however, that RpS5a encodes RPS5; the status of the gene encoded by λDmS18 remains uncertain.