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Gene Dmel\Taf6

General Information
Symbol	Dmel\Taf6	Species	D. melanogaster
Name	TBP-associated factor 6	Annotation symbol	CG32211
Feature type	protein_coding_gene	FlyBase ID	FBgn0010417
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	TAFII60, taf60, dTAFII60, dTAFII62, TFIID, TAFII60, TAF60/62, dTAF62, TAFII60/62, TAFII62
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	76B9-76B9	Sequence location	3L:19,592,187..19,594,482 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TBP-associated factor 6 is referred to in FlyBase by the symbol Dmel\Taf6 (CG32211, FBgn0010417). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly. There is experimental evidence that it is involved in the biological process: transcription from RNA polymerase II promoter; neurogenesis. 16 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; external compound sense organ; spermatocyte; egg; cell part; cell; peripheral nervous system; rhabdomere; adult segment. It has one annotated transcript and one annotated polypeptide. Protein features are: Armadillo-like helical; Domain of unknown function DUF1546; Histone-fold; TATA box binding protein associated factor (TAF). Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626563_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Taf6. Gene sequence location is 3L:19592187..19594482.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Alleles Taf6[O29] Taf6[B11] References Vorobyeva et al., 2009, Cell Cycle 8(14): 2152--2156 Sequence features HFA11299 BKN21202 Controlled Vocabulary Terms sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly transcription from RNA polymerase II promoter
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 76B9-76B9   Limits computationally determined from genome sequence between P{EP}MESR6EP3142 and P{lacW}l(3)L3809L3809
Experimentally Determined Cytological Location
Cytogenetic map Notes References 76B9-76B10     (Weinzierl, 1993.12.31) 76B9-76B10   (determined by in situ hybridisation)   (Weinzierl et al., 1993) 76C-76D   On basis of meiotic mapping (details unspecified).   (Karim et al., 1996)
Experimentally Determined Recombination Data
Location	3- (Karim et al., 1996)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Taf6 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Taf6-RA FBtr0074941  NM_079437   2057   606
Additional Transcript Data & Comments
Reported size (kB)	2.1 (unknown) (Weinzierl et al., 1993) 2.0 (longest cDNA) (Kokubo et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Taf6-PA   FBpp0074709   65.7   606   9.33     NP_524161   AAF49139
Additional Polypeptide Data & Comments
Reported size (kDa)	592 (aa); 64 (kD predicted) (Kokubo et al., 1994, Weinzierl et al., 1993) 60 (kD) (Dynlacht et al., 1991)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • TATA box binding protein associated factor (TAF) (IPR004823) Histone-fold (IPR009072) Domain of unknown function DUF1546 (IPR011442) Armadillo-like helical (IPR011989) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1TAF
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202405   AA202981   AA263313   AA263450   AA391286   AA391445   AA695502   AA735126   AA820682   AE014296 AAF49139   AI294601   AI455493   AI516120   AI531755   AI533156   AY051702 AAK93126   BI234604   BI352360   BI487076   BI487160   BI625325   BI625379   BI627068   BI639912   CK660314   CO185684   CO196642   CO262727   CO265801   CO267609   CO275676   CO278852   CO282522   CO284069   CO299655   CO315799   CO320186   CZ489669   EC064566   EC082003   EC083630   EC089301   EC215997   EC221770   EC222487   EC222657   EC228763   EC228976   EC235182   EC245099   EL874462   GH769897   L25443 AAA16536   U06459 AAC46480
UniProtKB/Swiss-Prot	P49847
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0010417    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0010417 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0010417 expression level Developmental Stage   Expression Level embryo 00-02hr    3546 embryo 02-04hr    5787 embryo 04-06hr    5135 embryo 06-08hr    4518 embryo 08-10hr    4284 embryo 10-12hr    3474 embryo 12-14hr    2175 embryo 14-16hr    2094 embryo 16-18hr    1665 embryo 18-20hr    1694 embryo 20-22hr    2127 embryo 22-24hr    1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6    1425 larva L3 puffstage 7-9    1668 white prepupae new    1797 white prepupae 12hr    2124 white prepupae 24hr    2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day    1407 adult female 05day    1885 adult female 30day    2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3546) embryo 02-04hr  (5787) embryo 04-06hr  (5135) embryo 06-08hr  (4518) embryo 08-10hr  (4284) embryo 10-12hr  (3474) embryo 12-14hr  (2175) embryo 14-16hr  (2094) embryo 16-18hr  (1665) embryo 18-20hr  (1694) embryo 20-22hr  (2127) embryo 22-24hr  (1970) larva L1  (901) larva L2  (698) larva L3 12hr old  (738) larva L3 puffstage 1-2  (1030) larva L3 puffstage 3-6  (1425) larva L3 puffstage 7-9  (1668) white prepupae new  (1797) white prepupae 12hr  (2124) white prepupae 24hr  (2456) pupae 2d postWPP  (1305) pupae 3d postWPP  (750) pupae 4d postWPP  (853) adult male 01day  (849) adult male 05day  (974) adult male 30day  (816) adult female 01day  (1407) adult female 05day  (1885) adult female 30day  (2044) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3546) embryo 02-04hr  (5787) embryo 04-06hr  (5135) embryo 06-08hr  (4518) embryo 08-10hr  (4284) embryo 10-12hr  (3474) embryo 12-14hr  (2175) embryo 14-16hr  (2094) embryo 16-18hr  (1665) embryo 18-20hr  (1694) embryo 20-22hr  (2127) embryo 22-24hr  (1970) larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6  1425 larva L3 puffstage 7-9  (1668) white prepupae new  (1797) white prepupae 12hr  (2124) white prepupae 24hr  (2456) pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day  1407 adult female 05day  (1885) adult female 30day  (2044) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3546 embryo 02-04hr    5787 embryo 04-06hr    5135 embryo 06-08hr    4518 embryo 08-10hr    4284 embryo 10-12hr    3474 embryo 12-14hr    2175 embryo 14-16hr    2094 embryo 16-18hr    1665 embryo 18-20hr    1694 embryo 20-22hr    2127 embryo 22-24hr    1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6    1425 larva L3 puffstage 7-9    1668 white prepupae new    1797 white prepupae 12hr    2124 white prepupae 24hr    2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day    1407 adult female 05day    1885 adult female 30day    2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3546 embryo 02-04hr    5787 embryo 04-06hr    5135 embryo 06-08hr    4518 embryo 08-10hr    4284 embryo 10-12hr    3474 embryo 12-14hr    2175 embryo 14-16hr    2094 embryo 16-18hr    1665 embryo 18-20hr    1694 embryo 20-22hr    2127 embryo 22-24hr    1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6    1425 larva L3 puffstage 7-9    1668 white prepupae new    1797 white prepupae 12hr    2124 white prepupae 24hr    2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day    1407 adult female 05day    1885 adult female 30day    2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0010417 expression level Developmental Stage   Expression Level embryo 00-02hr    3546 embryo 02-04hr    5787 embryo 04-06hr    5135 embryo 06-08hr    4518 embryo 08-10hr    4284 embryo 10-12hr    3474 embryo 12-14hr    2175 embryo 14-16hr    2094 embryo 16-18hr    1665 embryo 18-20hr    1694 embryo 20-22hr    2127 embryo 22-24hr    1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6    1425 larva L3 puffstage 7-9    1668 white prepupae new    1797 white prepupae 12hr    2124 white prepupae 24hr    2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day    1407 adult female 05day    1885 adult female 30day    2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3546) embryo 02-04hr  (5787) embryo 04-06hr  (5135) embryo 06-08hr  (4518) embryo 08-10hr  (4284) embryo 10-12hr  (3474) embryo 12-14hr  (2175) embryo 14-16hr  (2094) embryo 16-18hr  (1665) embryo 18-20hr  (1694) embryo 20-22hr  (2127) embryo 22-24hr  (1970) larva L1  (901) larva L2  (698) larva L3 12hr old  (738) larva L3 puffstage 1-2  (1030) larva L3 puffstage 3-6  (1425) larva L3 puffstage 7-9  (1668) white prepupae new  (1797) white prepupae 12hr  (2124) white prepupae 24hr  (2456) pupae 2d postWPP  (1305) pupae 3d postWPP  (750) pupae 4d postWPP  (853) adult male 01day  (849) adult male 05day  (974) adult male 30day  (816) adult female 01day  (1407) adult female 05day  (1885) adult female 30day  (2044) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3546) embryo 02-04hr  (5787) embryo 04-06hr  (5135) embryo 06-08hr  (4518) embryo 08-10hr  (4284) embryo 10-12hr  (3474) embryo 12-14hr  2175 embryo 14-16hr  2094 embryo 16-18hr  1665 embryo 18-20hr  1694 embryo 20-22hr  2127 embryo 22-24hr  1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6  1425 larva L3 puffstage 7-9  1668 white prepupae new  1797 white prepupae 12hr  2124 white prepupae 24hr  2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day  1407 adult female 05day  1885 adult female 30day  2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3546 embryo 02-04hr    5787 embryo 04-06hr    5135 embryo 06-08hr    4518 embryo 08-10hr    4284 embryo 10-12hr    3474 embryo 12-14hr    2175 embryo 14-16hr    2094 embryo 16-18hr    1665 embryo 18-20hr    1694 embryo 20-22hr    2127 embryo 22-24hr    1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6    1425 larva L3 puffstage 7-9    1668 white prepupae new    1797 white prepupae 12hr    2124 white prepupae 24hr    2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day    1407 adult female 05day    1885 adult female 30day    2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3546 embryo 02-04hr    5787 embryo 04-06hr    5135 embryo 06-08hr    4518 embryo 08-10hr    4284 embryo 10-12hr    3474 embryo 12-14hr    2175 embryo 14-16hr    2094 embryo 16-18hr    1665 embryo 18-20hr    1694 embryo 20-22hr    2127 embryo 22-24hr    1970 larva L1    901 larva L2    698 larva L3 12hr old    738 larva L3 puffstage 1-2    1030 larva L3 puffstage 3-6    1425 larva L3 puffstage 7-9    1668 white prepupae new    1797 white prepupae 12hr    2124 white prepupae 24hr    2456 pupae 2d postWPP    1305 pupae 3d postWPP    750 pupae 4d postWPP    853 adult male 01day    849 adult male 05day    974 adult male 30day    816 adult female 01day    1407 adult female 05day    1885 adult female 30day    2044 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0010417    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0010417 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0010417 expression level Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0010417 expression level Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    239.6 Larval Midgut    63.7 Larval Hindgut    128.3 Larval Malpighian Tubules    160.6 Larval Fat Body    100.1 Larval Salivary Gland    154.2 Larval Trachea    134.675 Larval Carcass    111 Adult Head    107.4 Adult Eye    91.7 Adult Brain    180.7 Adult Thoracic-Abdominal Ganglion    158.7 Adult Crop    116.3 Adult Midgut    57.7 Adult Hindgut    101.2 Adult Malpighian Tubules    103.8 Adult Fat Body    134.6 Adult Salivary Gland    127.1 Adult Heart    91.575 Adult VirginFemale Spermatheca    137 Adult InseminatedFemale Spermatheca    113.5 Adult Ovary    220.8 Adult Testis    61.5 Adult Male Accessory Gland    166.9 Adult Carcass    102.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    107.4   NA  Eye    91.7   NA  Brain    180.7   239.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    158.7   NA  Crop    116.3   63.7  Midgut    57.7   128.3  Hindgut    101.2   160.6  Malpighian Tubules    103.8   100.1  Fat Body    134.6   154.2  Salivary Gland    127.1   NA  Heart    91.575   134.675  Trachea    NA   NA  VirginFemale Spermatheca    137   NA  InseminatedFemale Spermatheca    113.5   NA  Ovary    220.8   NA  Testis    61.5   NA  Male Accessory Gland    166.9   111  Carcass    102.7 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010417 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32211-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Taf6 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | recessive | somatic clone Taf6XS-922 lethal, with Scer\GAL41407 Taf6GD10817 lethal | larval stage (with Taf61) Taf62 Taf63 Taf6XS-922 lethal | larval stage (with Taf62) Taf61 lethal | larval stage (with Taf63) Taf61 Taf6XS-922 lethal | larval stage (with Taf6XS-922) Taf61 Taf63 lethal | larval stage | recessive Taf6XS-922 lethal | pupal stage, with Scer\GAL4pnr-MD237 Taf6GD10817 lethal | pupal stage (with Taf62) Taf6XS-922 lethal | pupal stage (with Taf6XS-922) Taf62 lethal | recessive Taf61 Taf6B11 Taf6O29 Taf6XS-922 Sterility Allele female sterile, with Taf6hs.T:Zzzz\FLAG Taf6unspecified female sterile, with Taf6unspecified Taf6hs.T:Zzzz\FLAG female sterile | germline clone | recessive Taf6XS-922 male sterile, with Taf62/Taf6XS-922 Taf6hs.T:Zzzz\FLAG male sterile (with Taf62), with Taf6hs.T:Zzzz\FLAG Taf6XS-922 male sterile (with Taf6XS-922), with Taf6hs.T:Zzzz\FLAG Taf62 Other Phenotypes Allele decreased cell number | somatic clone | third instar larval stage Taf6B11 Taf6O29 melanotic mass phenotype (with Taf63) Taf6hs.T:Zzzz\FLAG melanotic mass phenotype (with Taf6hs.T:Zzzz\FLAG) Taf63 neuroanatomy defective | larval stage, with Scer\GAL41407 Taf6GD10817 visible, with Taf62/Taf6XS-922 Taf6hs.T:Zzzz\FLAG visible (with Taf62), with Taf6hs.T:Zzzz\FLAG Taf6XS-922 visible (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 visible (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG visible (with Taf6XS-922), with Taf6hs.T:Zzzz\FLAG Taf62 Phenotype manifest in Allele chorion, with Taf6hs.T:Zzzz\FLAG Taf6unspecified chorion, with Taf6unspecified Taf6hs.T:Zzzz\FLAG dorsal appendage, with Taf6hs.T:Zzzz\FLAG Taf6unspecified dorsal appendage, with Taf6unspecified Taf6hs.T:Zzzz\FLAG egg, with Taf6hs.T:Zzzz\FLAG Taf6unspecified egg, with Taf6unspecified Taf6hs.T:Zzzz\FLAG embryonic/larval brain | somatic clone | third instar larval stage Taf6B11 Taf6O29 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 Taf6GD10817 eye, with Taf62/Taf6XS-922 Taf6hs.T:Zzzz\FLAG eye (with Taf62), with Taf6hs.T:Zzzz\FLAG Taf6XS-922 eye (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 eye (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG eye (with Taf6XS-922), with Taf6hs.T:Zzzz\FLAG Taf62 eye photoreceptor cell (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 eye photoreceptor cell (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG eye photoreceptor cell | supernumerary (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 eye photoreceptor cell | supernumerary (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG ganglion mother cell | larval stage, with Scer\GAL41407 Taf6GD10817 melanotic mass (with Taf63) Taf6hs.T:Zzzz\FLAG melanotic mass (with Taf6hs.T:Zzzz\FLAG) Taf63 ommatidium (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 ommatidium (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG primary spermatocyte, with Taf62/Taf6XS-922 Taf6hs.T:Zzzz\FLAG primary spermatocyte (with Taf62), with Taf6hs.T:Zzzz\FLAG Taf6XS-922 primary spermatocyte (with Taf6XS-922), with Taf6hs.T:Zzzz\FLAG Taf62 rhabdomere (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 rhabdomere (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG spermatocyte, with Taf6hs.T:Zzzz\FLAG Taf6XS-922 spermatocyte, with Taf6XS-922 Taf6hs.T:Zzzz\FLAG testis, with Taf62/Taf6XS-922 Taf6hs.T:Zzzz\FLAG testis (with Taf62), with Taf6hs.T:Zzzz\FLAG Taf6XS-922 testis (with Taf6XS-922), with Taf6hs.T:Zzzz\FLAG Taf62 wing, with Taf62/Taf6XS-922 Taf6hs.T:Zzzz\FLAG wing (with Taf62), with Taf6hs.T:Zzzz\FLAG Taf6XS-922 wing (with Taf6hs.T:Zzzz\FLAG) Taf6XS-922 wing (with Taf6XS-922) Taf6hs.T:Zzzz\FLAG wing (with Taf6XS-922), with Taf6hs.T:Zzzz\FLAG Taf62
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Taf6 Class Mutagen Stocks Known lesion Taf61 ethyl methanesulfonate 2 Yes Taf6f06930 piggyBac transposase 2 -- Taf62 0 Yes Taf63 0 Yes Taf6B11 ethyl methanesulfonate 0 -- Taf6O29 ethyl methanesulfonate 0 -- Taf6unspecified 0 -- Taf6XS-922 loss of function allele X ray 0 Yes
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Taf6 Class Mutagen Stocks Known lesion Taf6GD10817 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf6KK101106 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Taf6Act5C.PF in vitro construct - regulatory fusion 0 Yes Taf6dsRNA.cWa in vitro construct - RNAi 0 Yes Taf6dsRNA.cWb in vitro construct - RNAi 0 Yes Taf6hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Taf6MtnA.CTR.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Taf6MtnA.NTR.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)A23 (Cooper et al., 2010) Df(3L)ash1A16 (Cooper et al., 2010) Df(3L)BSC20 (Cooper et al., 2010) Df(3L)BSC417 (Christensen et al., 2008.4.15, Cooper et al., 2010) Df(3L)ED228 (Cooper et al., 2010) Df(3L)Exel9007 (Ryder, 2004.4.26, Cooper et al., 2010) Df(3L)Exel9008 (Ryder, 2004.4.26, Cooper et al., 2010) Df(3L)Exel9009 (Ryder, 2004.4.26, Cooper et al., 2010) Df(3L)Exel9011 (Ryder, 2004.4.26, Cooper et al., 2010) Df(3L)kto2 (Cooper et al., 2010) Df(3L)pipeA13 (Cooper et al., 2010) Df(3L)XS533 (Cooper et al., 2010) Df(3L)XS543 (Cooper et al., 2010) Df(3L)XS572 (Cooper et al., 2010) Df(3L)XS705 (Cooper et al., 2010) Df(3L)XS917 (Cooper et al., 2010) Df(3L)XS2182 (Cooper et al., 2010)
Not disrupted in	Df(3L)25-21 (Cooper et al., 2010) Df(3L)BSC2 (Cooper et al., 2010) Df(3L)BSC445 (Cooper et al., 2010) Df(3L)ED4799 (Cooper et al., 2010) Df(3L)ED4858 (Cooper et al., 2010) Df(3L)Exel6135 (Cooper et al., 2010) Df(3L)Exel9045 (Cooper et al., 2010) Df(3L)Exel9046 (Cooper et al., 2010) Df(3L)kto22 (Cooper et al., 2010) Df(3L)XS411 (Cooper et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hsp70-eTAF60} NA UAS construct P{GD10817} NA P{KK101106} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}Taf6f06930 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
contributes_to sequence-specific core promoter binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly inferred from direct assay (Wright et al., 2006, Hansen and Tjian, 1995)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) transcription from RNA polymerase II promoter inferred from direct assay (Hansen and Tjian, 1995)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Wright et al., 2006, Hansen and Tjian, 1995) transcription factor TFIID complex inferred from direct assay (Hansen and Tjian, 1995) inferred from physical interaction with Taf4 (Wright et al., 2006)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR009072 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of sequence-specific DNA binding transcription factor activity inferred from electronic annotation with InterPro:IPR011442 (FlyBase Curators et al., 2004-) regulation of transcription, DNA-dependent non-traceable author statement (Swiss-Prot Project Members, 1996.6.1) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human TAF6L AND inferred from sequence or structural similarity with Saccharomyces TAF60 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1996.6.1) transcription factor TFIID complex inferred from sequence or structural similarity with human TAF6L AND inferred from sequence or structural similarity with Saccharomyces TAF60 (Aoyagi and Wassarman, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Taf6 allele Gene References Taf6XS-922 Dsor1 (Karim et al., 1996) phl (Karim et al., 1996) Ras85D (Karim et al., 1996, Sauer et al., 1996) sev (Maixner et al., 1998)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65404 DroID - A comprehensive database of gene and protein interactions. • FBgn0010417 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40134
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23708 Dere\GG16046 Dgri\GH16937 Dmoj\GI11798 Dper\GL20945 Dpse\GA16761 Dsec\GM19603 Dsim\GD14815 Dvir\GJ13501 Dwil\GK19071 Dyak\GE19612
Curated drosophilid orthologs	Dmir\CG9348-like   Dpse\GA16761   Dsim\Taf6 (Swanson et al., 2001)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006988-PA Aedes aegypti (mosquito) AGAP004254-PA Anopheles gambiae (mosquito) XP_393233.2 Apis mellifera (honey bee) XP_001947762.1 Acyrthosiphon pisum (pea aphid) At1g04950.1 Arabidopsis thaliana (wall cress) 13395 Batrachochytrium dendrobatidis (chytrid fungus) 206657 Branchiostoma floridae (lancelet) BGIBMGA007579-PA Bombyx mori (silk worm) ENSBTAP00000009230 Bos taurus (bovine) CAL0005584 Candida albicans (a form of yeast) CN11861 Caenorhabditis brenneri (small nematode) CE27222 Caenorhabditis elegans (worm) ENSCAFP00000021623 Canis familiaris (dog) CAGL0F07249g Candida glabrata (haploid yeast) CAGL0F07381g Candida glabrata (haploid yeast) ENSCINP00000009720 Ciona intestinalis (sea squirt) CMK081C Cyanidioschyzon merolae (red alga) cn05021 Cryptococcus neoformans (yeast-like fungus) CPIJ002544 Culex pipens (mosquito) ENSCPOP00000004602 Cavia porcellus (guinea pig) RP40157 Caenorhabditis remanei (nematode) ENSCSAVP00000006932 Ciona savignyi (sedentary tunicate) 183030 Capitella spl (polychaete worm) DDB0231094 Dictyostelium discoideum (amoeba) DEHA2F04356g Debaryomyces Hansenii (yeast) 244529 Daphnia pulex (daphnia) ENSDARP00000042900 Danio rerio (zebra fish) ENSECAP00000006873 Equus caballus (horse) XP_650399 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000027007 Gymnopilus aculeatus (mushroom) 93424 Hypsipyla robusta (cedar shoot borer) ENSP00000344537 Homo sapiens (human) ISCW010253-PA Ixodes scapularis (deer tick) KLLA0F10659g Kluyveromyces lactis (yeast) 175212 Lottia gigantea (owl limpet) ENSMODP00000005279 Monodelphis domestica (gray opossum) ENSMMUP00000017680 Macaca mulatta (rhesus monkey) ENSMUSP00000048016 Mus musculus (house mouse) 162513 Nematostella vectensis (starlet anemone) XP_001599379.1 Nasonia vitripennis (wasp) ENSOANP00000018970 Ornithorynchus anatinus (platypus) ENSORLP00000009712 Oryzias latipes (japanese killifish) LOC_Os01g32750.1 Oryza sativa (asian rice) 2139 Ostreococcus tauri (green alga) PHUM003666-PA Pediculus humanus (human body louse) PP01921 Pristionchus pacificus (nematode) 57083 Physcomitrella patens (moss) ENSPPYP00000019499 Pongo pygmaeus (orangutan) 82295 Phytophthora ramorum (sudden oak death pathogen) 136830 Phytophthora sojae (root rot pathogen) 246865 Populus trichocarpa (black cottonwood) ENSPTRP00000033329 Pan troglodytes (chimpanzee) ENSRNOP00000001829 Rattus norvegicus (norway rat) 4999755 Sorghum bicolor (sorghum) YGL112C Saccharomyces cerevisiae (budding yeast) Smp_061300 Schistosoma mansoni (trematode parasite) SPCC16C4.18c Schizosaccharomyces pombe (fission yeast) XP_781123.2 Strongylocentrotus purpuratus (purple sea urchin) 56085 Trichoplax adhaerens (metazoa) XP_001811805.1 Tribolium castaneum (red flour beetle) ENSTNIP00000014712 Tetraodon nigroviridis (green spotted pufferfish) TP03_0693 Theileria parva (parasitic protozoan) 3021 Thalassiosira pseudonana (marine diatom) ENSTRUP00000042118 Takifugu rubripes (pufferfish Takifugu) 3755.m00482 Tetrahymena thermophila (ciliate protozoa) 83996.m00287 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000008691 Xenopus tropicalis (frog) YALI0C05346g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10143 NV18184 LH19437 ISCW010253 FBgn0236951 FBgn0221069 FBgn0200725 FBgn0186488 FBgn0174503 JGI_V11_244529 FBgn0076775 FBgn0158540 FBgn0134557 FBgn0124408 FBgn0108281 FBgn0100702 CPIJ002544 BGIBMGA007579 ACYPI002370 GB15269 AGAP004254 AAEL006988 PHUM376110 GLEAN_13033
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	19025 w1118; PBac{WH}Taf6f06930/TM6B, Tb1 5046 Taf61 red1 e*/TM6B, Sb1 Tb1 ca1
Harvard	- PBac{WH}Taf6f06930
Kyoto	108006 Taf61 red1 e*/TM6B, Sb1 Tb1 ca1
VDRC	v108287 P{KK101106}VIE-260B v34452 w1118; P{GD10817}v34452
Genomic Clones ( 2 )
	BACR01D04 BACR23N10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 48 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD24529
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM02929 GM18962 LD01205 LD02576 LD03401 LD06463 LD07223 LD09885 LD10109 LD41876 LP08015 LP18779 RE30331 RE70581 RE70675 RH65667 RH65767 RH68260 SD03010 SD04886 SD22620
Other clones	15084 33317 38628 44972 50139 65033 97248 159507 177794 CK01.2B.A12 CK01.7D.H12 CK01.102B.C9 EK007027 EK010862 EK086209 EK118243 EK131908 EK143660 EK151059 EK160642 EK165158 EK176061 EK262459 EK282644 IP14463 RP01604
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010417 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40134
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) The proteins encoded by Taf4, Taf5, Taf6, e(y)1 and Taf12 form a core sub-complex of TFIID. (Wright et al., 2006) The N-terminal domain of the Taf6 protein is necessary and sufficient to stabilize TFIID. (Wright et al., 2006) Identified on the basis of genetic interaction with Ras85DV12.sev. (Karim et al., 1996) Taf6 does not exhibit histone acetyltransferase (HAT) activity in vitro. (Mizzen et al., 1996) Interacts, in vitro, with human thryroid-hormone receptor-β. (Petty et al., 1996) Taf6 acts as a positive regulator of sev transcription. Taf4 and Taf6 serve as coactivators responsible for both simple and synergistic transcription during development of the embryo. (Sauer et al., 1996) The amino terminal portions of e(y)1 and Taf6 adopt the canonical histone fold, consisting of two short α-helices flanking a long central α helix. e(y)1 and Taf6 form an intimate heterodimer by extensive hydrophobic contacts between the paired molecules. In solution and in the crystalline state the e(y)1/Taf6 complex exists as a heterotetramer, resembling the (His3/His4)2 heterotetrameric core of the histone octamer, suggesting that TFIID contains a histone octamer-like substructure. (Xie et al., 1996) Both of the Taf4 and Taf6 coactivators are required for bcd to recruit the Tbp-Taf complex to the promoter and direct synergistic activation of transcription. Contact between multiple activation domains for bcd and different targets within the TfIID complex can mediate transcriptional synergism. (Sauer et al., 1995) Mutagenesis studies in combination with protein binding experiments and reconstituted transcription reactions identified two independent activation domains of bcd that target different coactivator subunits (Taf4 and Taf6). (Sauer et al., 1995) The products of the Taf4 and Taf6 loci serve as coactivators to mediate transcriptional activation by the bcd and hb enhancer binding proteins. A quadruple complex containing Tbp, Taf1, Taf4 and Taf6 mediates transcriptional synergism by bcd and hb, whereas triple Tbp-Taf complexes lacking one or other coactivator failed to support synergistic activation. The concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during development. (Sauer et al., 1995) The assembly and purification of various functional Tbp TAF complexes demonstrate that Tbp plus the TAFs is sufficient to replace endogenous TFIID to support activated transcription in vitro. (Chen et al., 1994) TFIID subunit proteins and the Tbp protein can interact in pairwise combinations with several subunits in a network of interactions within TFIID. (Kokubo et al., 1994) The TFIID complex interacts with the promoters of Hsp70Bb, Hsp70Bc, Hsp26 and His3 making contacts at the TATA element, initiator, +18 and +28 regions. (Purnell et al., 1994) A number of polypeptides (encoded by Taf1, Taf4, Taf5, Taf6, e(y)1 and Taf12) that are tightly associated with Tbp and are native TFIID components have been purified. Protein blotting experiments suggest that one of these proteins, Taf1, interacts directly with Tbp, while the association between Tbp and the other proteins is either weak or is an indirect association via Taf1. (Kokubo et al., 1993) The Taf6 protein interacts directly with the e(y)1 protein. (Weinzierl et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079437 RefSeq (Proteins) • NP_524161 DDBJ / EMBL / GenBank • AA820682 AAA16536 AAC46480 AAF49139 AAK93126 AI455493 AY051702 CZ489669 L25443 U06459 Entrez Gene - A searchable database of RefSeq genes. • 40134 UniProtKB/Swiss-Prot • P49847
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • TATA box binding protein associated factor (TAF) (IPR004823) Histone-fold (IPR009072) Domain of unknown function DUF1546 (IPR011442) Armadillo-like helical (IPR011989) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1TAF
Linkouts
	BioGRID - A database of protein and genetic interactions • 65404 DroID - A comprehensive database of gene and protein interactions. • FBgn0010417 DRSC - Results from RNAi screens. • FBgn0010417 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010417 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32211-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010417 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40134 InParanoid A subset of ortholog calls from InParanoid. • AAEL006988-PA Aedes aegypti (mosquito) AGAP004254-PA Anopheles gambiae (mosquito) XP_393233.2 Apis mellifera (honey bee) XP_001947762.1 Acyrthosiphon pisum (pea aphid) At1g04950.1 Arabidopsis thaliana (wall cress) 13395 Batrachochytrium dendrobatidis (chytrid fungus) 206657 Branchiostoma floridae (lancelet) BGIBMGA007579-PA Bombyx mori (silk worm) ENSBTAP00000009230 Bos taurus (bovine) CAL0005584 Candida albicans (a form of yeast) CN11861 Caenorhabditis brenneri (small nematode) CE27222 Caenorhabditis elegans (worm) ENSCAFP00000021623 Canis familiaris (dog) CAGL0F07249g Candida glabrata (haploid yeast) CAGL0F07381g Candida glabrata (haploid yeast) ENSCINP00000009720 Ciona intestinalis (sea squirt) CMK081C Cyanidioschyzon merolae (red alga) cn05021 Cryptococcus neoformans (yeast-like fungus) CPIJ002544 Culex pipens (mosquito) ENSCPOP00000004602 Cavia porcellus (guinea pig) RP40157 Caenorhabditis remanei (nematode) ENSCSAVP00000006932 Ciona savignyi (sedentary tunicate) 183030 Capitella spl (polychaete worm) DDB0231094 Dictyostelium discoideum (amoeba) DEHA2F04356g Debaryomyces Hansenii (yeast) 244529 Daphnia pulex (daphnia) ENSDARP00000042900 Danio rerio (zebra fish) ENSECAP00000006873 Equus caballus (horse) XP_650399 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000027007 Gymnopilus aculeatus (mushroom) 93424 Hypsipyla robusta (cedar shoot borer) ENSP00000344537 Homo sapiens (human) ISCW010253-PA Ixodes scapularis (deer tick) KLLA0F10659g Kluyveromyces lactis (yeast) 175212 Lottia gigantea (owl limpet) ENSMODP00000005279 Monodelphis domestica (gray opossum) ENSMMUP00000017680 Macaca mulatta (rhesus monkey) ENSMUSP00000048016 Mus musculus (house mouse) 162513 Nematostella vectensis (starlet anemone) XP_001599379.1 Nasonia vitripennis (wasp) ENSOANP00000018970 Ornithorynchus anatinus (platypus) ENSORLP00000009712 Oryzias latipes (japanese killifish) LOC_Os01g32750.1 Oryza sativa (asian rice) 2139 Ostreococcus tauri (green alga) PHUM003666-PA Pediculus humanus (human body louse) PP01921 Pristionchus pacificus (nematode) 57083 Physcomitrella patens (moss) ENSPPYP00000019499 Pongo pygmaeus (orangutan) 82295 Phytophthora ramorum (sudden oak death pathogen) 136830 Phytophthora sojae (root rot pathogen) 246865 Populus trichocarpa (black cottonwood) ENSPTRP00000033329 Pan troglodytes (chimpanzee) ENSRNOP00000001829 Rattus norvegicus (norway rat) 4999755 Sorghum bicolor (sorghum) YGL112C Saccharomyces cerevisiae (budding yeast) Smp_061300 Schistosoma mansoni (trematode parasite) SPCC16C4.18c Schizosaccharomyces pombe (fission yeast) XP_781123.2 Strongylocentrotus purpuratus (purple sea urchin) 56085 Trichoplax adhaerens (metazoa) XP_001811805.1 Tribolium castaneum (red flour beetle) ENSTNIP00000014712 Tetraodon nigroviridis (green spotted pufferfish) TP03_0693 Theileria parva (parasitic protozoan) 3021 Thalassiosira pseudonana (marine diatom) ENSTRUP00000042118 Takifugu rubripes (pufferfish Takifugu) 3755.m00482 Tetrahymena thermophila (ciliate protozoa) 83996.m00287 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000008691 Xenopus tropicalis (frog) YALI0C05346g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40134 modMine - Data generated by the modENCODE project. • FBgn0010417 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10143 NV18184 LH19437 ISCW010253 FBgn0236951 FBgn0221069 FBgn0200725 FBgn0186488 FBgn0174503 JGI_V11_244529 FBgn0076775 FBgn0158540 FBgn0134557 FBgn0124408 FBgn0108281 FBgn0100702 CPIJ002544 BGIBMGA007579 ACYPI002370 GB15269 AGAP004254 AAEL006988 PHUM376110 GLEAN_13033
Synonyms & Secondary IDs ( 39 )
Reported As
Symbol Synonym	CG9348 (Aoyagi and Wassarman, 2000, ) CG32211 (Keleman et al., 2009.8.5, Chen et al., 2008) d60 (Pugh, 2000) dme-TAFII60 (Baxevanis et al., 1995) dmTAF6 (Muratoglu et al., 2003) dTAF6 (Guelman et al., 2006) dTAF62 (Nogales, 2000, Hoffmann et al., 1996) dTAFII60 (Gangloff et al., 2001, Veenstra and Wolffe, 2001, Albright and Tjian, 2000, Mantovani, 1999, Naar et al., 1998, Burke et al., 1998, Bell and Tora, 1999, Burke and Kadonaga, 1997, Mahanta et al., 1997, Felsenfeld, 1996, Damania and Alwine, 1996, Goodrich and Tjian, 1994, Weinzierl et al., 1993, Yokomori et al., 1993, Wright et al., 2006) dTAFII62 (Pugh, 2000, Burley, 1998, Maity and de Crombrugghe, 1998, Xie et al., 1996, Surridge, 1996, Nakatani et al., 1996) dTAFII262 (Mizzen et al., 1996) dTAFII62 (Saurin et al., 2001) l(3)76BDf (Cooper et al., 2010) p62 (Kokubo et al., 1994, Kokubo et al., 1993) SR3-4B (Sauer et al., 1996, Karim et al., 1996, ) TAF (Mueller and Tora, 2004) TAF6 (Tora, 2002, Maile et al., 2004, Marr et al., 2006, Wright et al., 2006, Wright and Tjian, 2009, Weake et al., 2009, Santoso and Kadonaga, 2006, Vorobyeva et al., 2009) Taf6 (Roy et al., 2007, Christensen et al., 2008.4.15, Somma et al., 2008, Vázquez et al., 2008, Chen et al., 2008, Cooper et al., 2010, Avet-Rochex et al., 2010, Roy et al., 2011, Wasbrough et al., 2010) Taf-6 (Slack et al., 2006) taf60 (Simonova and Modestova, 2003) Taf60 (Zinke et al., 2002, Egger et al., 2002, Bloomington Drosophila Stock Center, 1999.6.30, Struhl and Moqtaderi, 1998, Verrijzer et al., 1995, Purnell et al., 1994, Hori and Carey, 1994, Hoey et al., 1993, Cooper et al., 2010) TAF60 (Aoyagi et al., 2000, Aoyagi and Wassarman, 1999, Maixner et al., 1998, Schaeffer et al., 1998, Hansen et al., 1997, Farmer et al., 1996) TAF60/62 (Green, 2000, Aoyagi and Wassarman, 2000, Hahn, 1998) Taf62   TAFII (Arnosti, 2002) TAFII60 (Butler and Kadonaga, 2002, Tora, 2002, Wassarman and Sauer, 2001, Adams et al., 2000, Schock et al., 1999, Schaeffer et al., 1999, Mannervik et al., 1999, Sauer et al., 1998, Zhou et al., 1998, Niessing et al., 1997, Sauer et al., 1996, Sauer et al., 1995, Sauer et al., 1995, Hansen and Tjian, 1995, Aoyagi and Wassarman, 2001) TafII60 (Pham et al., 1999, Chen et al., 1994) TAFII60/62 (Shen et al., 1998, Tansey and Herr, 1997) TAFII62 (Tsukiyama and Wu, 1997, Wang and Brock, 2003) TAFII60 (Govind, 1999, Weinzierl, 1993.12.31, Dynlacht et al., 1993, Dynlacht et al., 1991) TAFII60(62) (Berk, 1999) TAFII62 (Schwartz and Pirrotta, 2007) TFIID (Butler and Kadonaga, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001) TFIID 62 (Nakatani, 1994.2.8)
Name Synonym	Suppressor of Ras85D 3-4B (Karim et al., 1996, ) TBP-associated factor (Schuettengruber, 2006) TBP-associated factor 6 (Roy et al., 2007) TBP-associated factor 60 (Dynlacht et al., 1993) TBP associated factor 60 (Dynlacht et al., 1991) TBP-associated factor 60kD
Secondary FlyBase IDs
	FBgn0011301 FBgn0016734 FBgn0052211
References ( 122 )
Generate a list of	All references relating to this gene ( 122 )
List References by type	Research paper  ( 58 ) Supplementary material  ( 6 ) Review  ( 32 ) Retraction  ( 1 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 5 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Letter  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 4 )
	Roy et al., 2011, Mol. Genet. Genomics 285(2): 113--123 Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex. [FBrf0212912] Avet-Rochex et al., 2010, BMC Dev. Biol. 10: 65 An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. [FBrf0211137] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Cooper et al., 2010, Genetics 185(3): 811--822 Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster. [FBrf0211329] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...