FlyBase Gene Report: Dmel\TfIIS

General Information

Symbol

Dmel\TfIIS

Species

D. melanogaster

Name

RNA polymerase II elongation factor

Annotation Symbol

CG3710

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010422

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. (UniProt, P20232)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

l(2)35Cf, DmS-II, BG:DS00929.12

Key Links

Genomic Location

Cytogenetic map

35B10-35B10

Sequence location

2L:15,058,919..15,060,757 [-]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:15058919..15060757

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR001222, InterPro:IPR006289

(InterPro Project Members, 2004-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR001222

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in DNA-templated transcription

inferred from electronic annotation with InterPro:IPR001222, InterPro:IPR003618, InterPro:IPR036575

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000159141

(GO Reference Genome Project, 2011-)

involved_in transcription elongation by RNA polymerase II

inferred from sequence or structural similarity with SGD:S000003011

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR006289

(InterPro Project Members, 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000159141

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from sequence or structural similarity with SGD:S000003011

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR003617, InterPro:IPR006289

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the TFS-II family. (P20232)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, P20232)

Summary (Interactive Fly)

critical for efficient release of stalled RNA Pol II from intrinsic stop sites in promoter regions - promotes transcriptional elongation and decreases pausing

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\TfIIS for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P20232)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

TfIIS-RA

FBtr0080722

NM_057619

1401

313

TfIIS-RB

FBtr0332475

NM_001273528

1773

313

Additional Transcript Data and Comments

Reported size (kB)

1.4 (northern blot)

(Marshall et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

TfIIS-PA

FBpp0080281

34.3

313

9.58

FBpp0304751

34.3

313

9.58

P20232

NP_001260457

AGB92992

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

313 aa isoforms: TfIIS-PA, TfIIS-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

313 (aa)

(Oh et al., 1995)

313 (aa); 34.3 (kD)

(Marshall et al., 1990)

36 (kD)

(Sluder et al., 1989)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TfIIS using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TfIIS_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.78

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

TfIIS transcripts are present in Kc cell RNA.

(Marshall et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\TfIIS in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\TfIIS

View exonic expression by tissue for Dmel\TfIIS

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010422

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34883/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010422

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010422

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010422

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3710

Flygut - An atlas of the Drosophila adult midgut

FBgn0010422

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\TfIIS

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of TfIIS

Transgenic constructs containing regulatory region of TfIIS

Aberrations (Deficiencies and Duplications) ( 106 )

Inferred from experimentation ( 106 )

Gene disrupted in

Df(2L)64j

(Roote, 2000.7.3, Ashburner et al., 1999, Iyengar et al., 1999, Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)A48

(Cai et al., 2001, Fossett et al., 1994)

Df(2L)A72

(Fossett et al., 1994, Ashburner et al., 1990)

Df(2L)A215

(Fossett et al., 1994)

Df(2L)A220

(Fossett et al., 1994)

Df(2L)A246

(Fossett et al., 1994)

Df(2L)A263

(Furukawa et al., 1992)

Df(2L)A264

(Fossett et al., 1994)

Df(2L)A267

(Cai et al., 2001, Fossett et al., 1994, Ashburner et al., 1990)

Df(2L)A337

(Fossett et al., 1994)

Df(2L)A376

(Fossett et al., 1994)

Df(2L)A400

(Fossett et al., 1994)

Df(2L)Adh-nBR101

(Fossett et al., 1994)

Df(2L)Adh-nBR102

(Fossett et al., 1994)

Df(2L)Adh-nBR103

(Fossett et al., 1994)

Df(2L)Adh-nBR105

(Fossett et al., 1994)

Df(2L)Adh-nBR118

(Fossett et al., 1994)

Df(2L)Adh-nBR120

(Fossett et al., 1994)

Df(2L)Adh-nBR122

(Fossett et al., 1994)

Df(2L)Adh-nBR125

(Fossett et al., 1994)

Df(2L)Adh-nBR126

(Fossett et al., 1994)

Df(2L)Adh-nBR128

(Fossett et al., 1994)

Df(2L)Adh-nBR129

(Fossett et al., 1994)

Df(2L)Adh-nBR130

(Fossett et al., 1994)

Df(2L)Adh-nBR131

(Fossett et al., 1994)

Df(2L)b80e4

(Ashburner et al., 1990)

Df(2L)b84h50

(Furukawa et al., 1992)

Df(2L)el20

(Ashburner et al., 1990)

Df(2L)el28

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)Exel7061

(Ryder, 2004.4.26)

Df(2L)Exel7062

(Ryder, 2004.4.26)

Df(2L)fn5

(Furukawa et al., 1992)

Df(2L)fn26

(Ashburner et al., 1990)

Df(2L)fn27

(Ashburner et al., 1990)

Df(2L)n78l3

(Ashburner et al., 1990)

Df(2L)noc11

(Ashburner et al., 1990)

Df(2L)osp29

(Cai et al., 2001, Furukawa et al., 1992)

Df(2L)osp38

(Ashburner et al., 1990)

Df(2L)Sco7

(Ashburner et al., 1990)

Df(2L)TE35BC-3

(Cai et al., 2001, Ashburner et al., 1990)

Df(2L)TE35BC-4

(Ashburner et al., 1990)

Df(2L)TE35BC-24

(Iyengar et al., 1999)

Df(2L)TE35BC-34

(Ashburner et al., 1990)

Df(2L)TE35D-20

(Ashburner et al., 1990)

Df(2L)TE35D-23

(Ashburner et al., 1990)

In(2L)el18

(Ashburner et al., 1990)

In(2LR)noc^4LSco^rv9R

(Cai et al., 2001)

Tp(2;2)A446

(Fossett et al., 1994)

Gene not disrupted in

Df(2L)A47

(Fossett et al., 1994)

Df(2L)A63

(Fossett et al., 1994)

Df(2L)A178

(Fossett et al., 1994)

Df(2L)A217

(Fossett et al., 1994)

Df(2L)A245

(Fossett et al., 1994)

Df(2L)A266

(Fossett et al., 1994)

Df(2L)A379

(Fossett et al., 1994)

Df(2L)A445

(Fossett et al., 1994)

Df(2L)Adh-nBR100

(Fossett et al., 1994)

Df(2L)Adh-nBR106

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR107

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR117

(Fossett et al., 1994)

Df(2L)Adh-nBR119

(Fossett et al., 1994)

Df(2L)Adh-nBR121

(Fossett et al., 1994)

Df(2L)Adh-nBR127

(Fossett et al., 1994)

Df(2L)ARR1

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)b84a3

(Furukawa et al., 1992)

Df(2L)b84a7

(Furukawa et al., 1992)

Df(2L)fn12

(Ashburner et al., 1990)

Df(2L)H20

(Ashburner et al., 1990)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Ashburner et al., 1990)

Df(2L)RA5

(Ashburner et al., 1990)

Df(2L)rd9

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)RM5

(Ashburner et al., 1990)

Df(2L)RN2

(Ashburner et al., 1990)

Df(2L)Sco^rv16

(Ashburner et al., 1990)

Df(2L)Sco^rv18

(Ashburner et al., 1990)

Df(2L)Sco^rv19

(Ashburner et al., 1990)

Df(2L)Sco^rv25

(Ashburner et al., 1990)

Df(2L)SS1

(Ashburner et al., 1990)

Df(2L)TE35BC-7

(Furukawa et al., 1992)

Df(2L)TE35BC-28

(Furukawa et al., 1992)

Df(2L)TE35D-1

(Ashburner et al., 1990)

Df(2L)TE35D-2

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-3

(Ashburner et al., 1990)

Df(2L)TE35D-4

(Ashburner et al., 1990)

Df(2L)TE35D-6

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-13

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-14

(Ashburner et al., 1990)

Df(2L)TE35D-15

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-16

(Ashburner et al., 1990)

Df(2L)TE35D-18

(Furukawa et al., 1992)

Df(2L)TE35D-19

(Ashburner et al., 1990)

Df(2L)TE35D-21

(Ashburner et al., 1990)

Df(2L)TE35D-22

(Cai et al., 2001, Ashburner et al., 1990)

In(2L)dpp^s22Ldpp^d36R

(Ashburner et al., 1990)

In(2LR)Sco^rv1-DV7

(Ashburner et al., 1990)

T(2;3)Sco^rv7

(Ashburner et al., 1990)

Gene duplicated in

Dp(2;1)Sco^rv23

(Ashburner et al., 1990)

Dp(2;2)GYL

(Ashburner et al., 1990)

Dp(2;2)TE35B-54^L

(Ashburner et al., 1990)

Dp(2;3)osp³

(Ashburner et al., 1990)

In(2L)C163.41^LC158^R

(Ashburner et al., 1990)

In(2L)Sco^rv17LC158^R

(Ashburner et al., 1990)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

TfIIS^GD12773

lethal | recessive

TfIIS^unspecified

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

TfIIS^GD12773

viable, with Scer\GAL4^Mef2.PR

TfIIS^GD12773

viable, with Scer\GAL4^pnr-MD237

TfIIS^GD12773

viable, with Scer\GAL4^tin.CΔ4

TfIIS^GD12773

Phenotype manifest in

Allele

wing

TfIIS^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (12)

Hsap\TCEA1

12 of 14

Yes

Hsap\TCEA3

12 of 14

Yes

Hsap\TCEA2

11 of 14

Yes

Hsap\TCEANC

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Tcea1

12 of 14

Yes

Rnor\Tcea2

12 of 14

Yes

Rnor\Tcea3

12 of 14

Yes

Rnor\LOC498453

11 of 14

Yes

Rnor\Tceanc

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (13)

Mmus\Tcea2

12 of 14

Yes

Mmus\Tcea3

12 of 14

Yes

Mmus\Tcea1

11 of 14

Yes

Mmus\Tceanc

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\tcea1

7 of 13

Yes

Xtro\tcea3

5 of 13

Yes

Xtro\plscr4

3 of 13

Yes

Xtro\tceanc

3 of 13

Yes

Xtro\LOC100490835

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (14)

Drer\tcea1

12 of 14

Yes

Drer\tcea2

12 of 14

Yes

Drer\tcea3

9 of 14

Yes

Drer\polr2i

2 of 14

Drer\polr3k

2 of 14

Drer\tceanc2

2 of 14

Yes

Drer\tceanc

2 of 14

Yes

Drer\col27a1a

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\T24H10.1

13 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP009035

12 of 12

Yes

Agam\AgaP_AGAP002375

2 of 12

Agam\AgaP_AGAP008393

2 of 12

Agam\AgaP_AGAP004866

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\TFIIS

11 of 13

Yes

Atha\AT2G42725

3 of 13

Yes

Atha\AT5G42325

3 of 13

Yes

Atha\AT1G01210

2 of 13

Atha\AT2G25640

2 of 13

Yes

Atha\AT3G10820

2 of 13

Yes

Atha\AT3G25940

2 of 13

Atha\AT4G07950

2 of 13

Atha\AT4G18720

2 of 13

Yes

Atha\AT5G09850

2 of 13

Yes

Atha\AT5G11430

2 of 13

Yes

Atha\AT5G25520

2 of 13

Yes

Atha\NRPB9A

2 of 13

Atha\NRPB9B

2 of 13

Atha\AT2G19910

1 of 13

Yes

Atha\AT2G42730

1 of 13

Yes

Atha\AT5G05140

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\DST1

11 of 13

Yes

Scer\BYE1

4 of 13

Yes

Scer\RPA12

2 of 13

Scer\RPB9

2 of 13

Scer\RPC11

2 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\tfs1

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:TfIIS. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

10 of 13

2 of 13

2 of 13

2 of 13

pps

2 of 13

EloA

1 of 13

MED26

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TCEA1; transcription elongation factor A1

12 of 14

601425

TCEA3; transcription elongation factor A3

12 of 14

604128

TCEA2; transcription elongation factor A2

11 of 14

604784

TCEANC; transcription elongation factor A N-terminal and central domain containing

4 of 14

301084

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

TfIIS - CG17118

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Rhee et al., 2014, Guruharsha et al., 2011)

TfIIS - CG4854

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014, Guruharsha et al., 2011)

TfIIS - Mef2

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

TfIIS - TfIIFβ

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60901

DroID - A comprehensive database of gene and protein interactions.

FBgn0010422

MIST (protein-protein) - An integrated Molecular Interaction Database

34883

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B10-35B10

Sequence location

2L:15,058,919..15,060,757 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B10-35B10

Limits computationally determined from genome sequence between P{lacW}Su(H)^k07904 and P{EP}vig^EP812&P{PZ}stc⁰⁵⁴⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35B-35B

(determined by in situ hybridisation)

(Oh et al., 1995)

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-47.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (87)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD34766

Other clones

DPiM_LD34766

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010422

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010422

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ni et al., 2008, Marshall et al., 1990)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

α-amanitin has an inhibitory effect on TfIIS-mediated transcript cleavage, but has no effect on elongation or pycophosphorolysis.

(Chafin et al., 1995)

TfIIS is necessary and sufficient to activate nascent transcript cleavage by RNA polymerase II during transcription of a dC-tailed template in vitro.

(Guo and Price, 1993)

2 alleles are discussed but are not named.

(Ashburner et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: TfIIS l(2)35Cf

(Ashburner et al., 1999)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: TfIIS CG3710

(Kodira, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

BG:DS00929.12

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG3710

(National Institute of Genetics Fly Stocks, 2022-, Çiçek et al., 2016, Chen et al., 2012, Ni et al., 2011.7.19, Zhang et al., 2011, Ni et al., 2010.12.1, Zhang et al., 2010, Keleman et al., 2009.8.5, de Wit et al., 2005)

DmS-II

(Chen et al., 1996, Chafin et al., 1995, Guo and Price, 1993, Kephart et al., 1992, Price, 1990.7.3)

DmSII

IIS

(Jacobs et al., 2020)

RnpSII

TFIIS

(Nechaev et al., 2010, Ardehali et al., 2009, Ni et al., 2008, Kim and Lis, 2005, Jin et al., 2004, Mizuguchi et al., 2001, Oh et al., 2001, Park et al., 2001, Reese et al., 2000, Oh et al., 1995)

TFIISA

(Tinker et al., 1998)

TFS-II

(Mushegian et al., 1998)

TfIIS

(Peng et al., 2024, National Institute of Genetics Fly Stocks, 2022-, Transgenic RNAi Project members, 2017-, Handler et al., 2013, Handler et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Dietzl et al., 2007, de Wit et al., 2005)

br52

(Furukawa et al., 1992)

l(2)35Cf

(Roote, 2000.6.13, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Roote, 1996.1.11, Ashburner, 1995.4.11, Roote, 1995.5.4, Spradling et al., 1995, Ashburner et al., 1990)

l(2)35cF

(Iyengar et al., 1999)

l(2)br52

l35Cf

(Fossett et al., 1994)

Name Synonyms

RNA polymerase II elongation factor

lethal(2)35Cf

transcription cleavage factor IIS

(Nechaev et al., 2010)

Secondary FlyBase IDs

FBgn0000474
FBgn0001982

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 65 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34883

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P20232

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P20232

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PFV9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P20232

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010422

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34883/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010422

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010422

FlyMine - An integrated database for Drosophila genomics

FBgn0010422

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3710

MARRVEL_MODEL - MARRVEL (model organism gene)

34883

Linkouts

BioGRID - A database of protein and genetic interactions.

60901

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010422

DroID - A comprehensive database of gene and protein interactions.

FBgn0010422

DRSC - Results frm RNAi screens

FBgn0010422

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TfIIS_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010422

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010422

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3710

Flygut - An atlas of the Drosophila adult midgut

FBgn0010422

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0010422

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014756

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

34883

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (95)

The following external sites may use different assemblies or annotations than FlyBase.

1.78

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