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Gene Dmel\TfIIS

General Information
Symbol	Dmel\TfIIS	Species	D. melanogaster
Name	RNA polymerase II elongation factor	Annotation symbol	CG3710
Feature type	protein_coding_gene	FlyBase ID	FBgn0010422
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	l(2)35Cf, DmS-II, BG:DS00929.12
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	35B10-35B10	Sequence location	2L:15,058,919..15,060,757 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene RNA polymerase II elongation factor is referred to in FlyBase by the symbol Dmel\TfIIS (CG3710, FBgn0010422). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: DNA binding; zinc ion binding. Based on sequence similarity, it is predicted to be involved in the biological process: regulation of transcription, DNA-dependent. 9 alleles are reported. The phenotype of these alleles is annotated with: wing. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Transcription elongation factor S-II, central domain; Transcription elongation factor S-IIM; Transcription elongation factor, TFIIS; Transcription elongation factor, TFIIS-related; Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type; Transcription factor IIS, N-terminal; Zinc finger, TFIIS-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval fat body, larval salivary gland, larval trachea, adult ovary, adult male reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1633430_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of TfIIS. Gene sequence location is 2L:15058919..15060757.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 35B10-35B10   Limits computationally determined from genome sequence between P{lacW}Su(H)k07904 and P{EP}vigEP812&P{PZ}stc05441
Experimentally Determined Cytological Location
Cytogenetic map Notes References 35B-35B   (determined by in situ hybridisation)   (Oh et al., 1995)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\TfIIS for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) TfIIS-RA FBtr0080722  NM_057619   1401   313 TfIIS-RB FBtr0332475  NM_001273528   1773   313
Additional Transcript Data & Comments
Reported size (kB)	1.4 (northern blot) (Marshall et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein TfIIS-PA   FBpp0080281   34.3   313   9.58     NP_476967   AAF53436 TfIIS-PB   FBpp0304751   34.3   313   9.58     NP_001260457   AGB92992
Additional Polypeptide Data & Comments
Reported size (kDa)	313 (aa); 34.3 (kD) (Marshall et al., 1990) 313 (aa) (Oh et al., 1995) 36 (kD) (Sluder et al., 1989)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, TFIIS-type (IPR001222) Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type (IPR003617) Transcription elongation factor S-II, central domain (IPR003618) Transcription elongation factor, TFIIS (IPR006289) Transcription elongation factor, TFIIS-related (IPR016492) Transcription elongation factor S-IIM (IPR017890) Transcription factor IIS, N-terminal (IPR017923)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440684   AA538819   AA695486   AA695587   AA697244   AA802194   AA951789   AA979056   AA990842   AE014134 AAF53436   AC092238   AE003412 AAF44916   AE014134 AGB92992   AI386490   AW943246   AY051843 AAK93267   BF504732   BI173395   BI229808   BI238947   BI371953   BI483019   BP542617   BP557472   BP557473   CO179073   CO179076   CO189865   CO268881   CO305376   CO308308   CO316691   EC057958   EC066240   EC069826   EC081206   EC208566   EC214622   EC215383   EV583734   EV588254   GH748090   GH758151   GH771987   GH776533   GH777671   GH778367   GH782721   GH784540   GH788888   GH790936   GH794960   GH802786   GH807081   GH809318   GH810548   GH813046   GH821227   GH822193   GH837640   GH838344   GH849479   GH855897   GH855938   GH857568   GH858367   GH864761   GH866718   GH870430   GH871188   GH874847   GH876243   GH879682   GH882389   GH887103   GH890215   GH893928   GH895101   GH900856   GH901421   GH904849   GH907898   GH909241   GH909373   GH910149   GH911004   GH920780   GH922198   GH927255   GH930237   GH931077   GH931487   GH938482   L26091 AAA92864   X53670 CAA37710
UniProtKB/Swiss-Prot	P20232
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
	TfIIS transcripts are present in Kc cell RNA. (Marshall et al., 1990)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval fat body, larval salivary gland, larval trachea, adult ovary, adult male reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    339.55 Larval Midgut    82.7 Larval Hindgut    86 Larval Malpighian Tubules    97.7 Larval Fat Body    110.2 Larval Salivary Gland    118.1 Larval Trachea    129.8 Larval Carcass    98.45 Adult Head    85 Adult Eye    227 Adult Brain    104.1 Adult Thoracic-Abdominal Ganglion    104 Adult Crop    95.7 Adult Midgut    72.9 Adult Hindgut    72 Adult Malpighian Tubules    84.6 Adult Fat Body    60.6 Adult Salivary Gland    94.5 Adult Heart    67.575 Adult VirginFemale Spermatheca    87.1 Adult InseminatedFemale Spermatheca    94.9 Adult Ovary    321.2 Adult Testis    101.6 Adult Male Accessory Gland    139.3 Adult Carcass    67.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    85   NA  Eye    227   NA  Brain    104.1   339.55  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    104   NA  Crop    95.7   82.7  Midgut    72.9   86  Hindgut    72   97.7  Malpighian Tubules    84.6   110.2  Fat Body    60.6   118.1  Salivary Gland    94.5   NA  Heart    67.575   129.8  Trachea    NA   NA  VirginFemale Spermatheca    87.1   NA  InseminatedFemale Spermatheca    94.9   NA  Ovary    321.2   NA  Testis    101.6   NA  Male Accessory Gland    139.3   98.45  Carcass    67.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    83 central nervous system, larvae L3    71 central nervous system, pupae P8    41 head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae    34 fat body, pupae P8    60 carcass, larvae L3 wandering    41 carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    69 ovary, mated 4-day female    75 testis, mated 4-day male    68 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (83) central nervous system, larvae L3  (71) central nervous system, pupae P8  (41) head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae  (34) fat body, pupae P8  (60) carcass, larvae L3 wandering  (41) carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female  (69) ovary, mated 4-day female  (75) testis, mated 4-day male  (68) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    83 central nervous system, larvae L3    71 central nervous system, pupae P8    41 head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae    34 fat body, pupae P8    60 carcass, larvae L3 wandering    41 carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    69 ovary, mated 4-day female    75 testis, mated 4-day male    68 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    83 central nervous system, larvae L3    71 central nervous system, pupae P8    41 head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae    34 fat body, pupae P8    60 carcass, larvae L3 wandering    41 carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    69 ovary, mated 4-day female    75 testis, mated 4-day male    68 accessory gland, mated 4-day male    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    83 central nervous system, larvae L3    71 central nervous system, pupae P8    41 head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae    34 fat body, pupae P8    60 carcass, larvae L3 wandering    41 carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    69 ovary, mated 4-day female    75 testis, mated 4-day male    68 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (83) central nervous system, larvae L3  (71) central nervous system, pupae P8  (41) head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae  (34) fat body, pupae P8  (60) carcass, larvae L3 wandering  (41) carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female  (69) ovary, mated 4-day female  (75) testis, mated 4-day male  (68) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    83 central nervous system, larvae L3    71 central nervous system, pupae P8    41 head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae    34 fat body, pupae P8    60 carcass, larvae L3 wandering    41 carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    69 ovary, mated 4-day female    75 testis, mated 4-day male    68 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    83 central nervous system, larvae L3    71 central nervous system, pupae P8    41 head, virgin 1-day female    18 head, virgin 4-day female    20 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    23 head, mated 4-day male    25 head, mated 20-day male    25 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    13 digestive system, larvae L3 wandering    22 digestive system, 1-day adult    22 digestive system, 4-day adult    23 digestive system, 20-day adult    15 fat body, larvae L3 wandering    11 fat body, white prepupae    34 fat body, pupae P8    60 carcass, larvae L3 wandering    41 carcass, 1-day adult    11 carcass, 4-day adult    15 carcass, 20-day adult    15 ovary, virgin 4-day female    69 ovary, mated 4-day female    75 testis, mated 4-day male    68 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    101 embryo 04-06hr    73 embryo 06-08hr    73 embryo 08-10hr    58 embryo 10-12hr    54 embryo 12-14hr    38 embryo 14-16hr    30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1    30 larva L2    28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    38 white prepupae new    37 white prepupae 12hr    47 white prepupae 24hr    53 pupae 2d postWPP    37 pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day    32 adult female 05day    53 adult female 30day    56 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (82) embryo 02-04hr  (101) embryo 04-06hr  (73) embryo 06-08hr  (73) embryo 08-10hr  (58) embryo 10-12hr  (54) embryo 12-14hr  (38) embryo 14-16hr  (30) embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1  (30) larva L2  (28) larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6  (30) larva L3 puffstage 7-9  (38) white prepupae new  (37) white prepupae 12hr  (47) white prepupae 24hr  (53) pupae 2d postWPP  (37) pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day  (32) adult female 05day  (53) adult female 30day  (56) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr  101 embryo 04-06hr    73 embryo 06-08hr    73 embryo 08-10hr    58 embryo 10-12hr    54 embryo 12-14hr    38 embryo 14-16hr    30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1    30 larva L2    28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    38 white prepupae new    37 white prepupae 12hr    47 white prepupae 24hr    53 pupae 2d postWPP    37 pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day    32 adult female 05day    53 adult female 30day    56 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    101 embryo 04-06hr    73 embryo 06-08hr    73 embryo 08-10hr    58 embryo 10-12hr    54 embryo 12-14hr    38 embryo 14-16hr    30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1    30 larva L2    28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    38 white prepupae new    37 white prepupae 12hr    47 white prepupae 24hr    53 pupae 2d postWPP    37 pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day    32 adult female 05day    53 adult female 30day    56 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    101 embryo 04-06hr    73 embryo 06-08hr    73 embryo 08-10hr    58 embryo 10-12hr    54 embryo 12-14hr    38 embryo 14-16hr    30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1    30 larva L2    28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    38 white prepupae new    37 white prepupae 12hr    47 white prepupae 24hr    53 pupae 2d postWPP    37 pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day    32 adult female 05day    53 adult female 30day    56 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (82) embryo 02-04hr  (101) embryo 04-06hr  (73) embryo 06-08hr  (73) embryo 08-10hr  (58) embryo 10-12hr  (54) embryo 12-14hr  (38) embryo 14-16hr  30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1  30 larva L2  28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6  30 larva L3 puffstage 7-9  (38) white prepupae new  (37) white prepupae 12hr  (47) white prepupae 24hr  (53) pupae 2d postWPP  (37) pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day  32 adult female 05day  (53) adult female 30day  (56) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr  101 embryo 04-06hr    73 embryo 06-08hr    73 embryo 08-10hr    58 embryo 10-12hr    54 embryo 12-14hr    38 embryo 14-16hr    30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1    30 larva L2    28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    38 white prepupae new    37 white prepupae 12hr    47 white prepupae 24hr    53 pupae 2d postWPP    37 pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day    32 adult female 05day    53 adult female 30day    56 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    101 embryo 04-06hr    73 embryo 06-08hr    73 embryo 08-10hr    58 embryo 10-12hr    54 embryo 12-14hr    38 embryo 14-16hr    30 embryo 16-18hr    23 embryo 18-20hr    15 embryo 20-22hr    14 embryo 22-24hr    14 larva L1    30 larva L2    28 larva L3 12hr old    18 larva L3 puffstage 1-2    22 larva L3 puffstage 3-6    30 larva L3 puffstage 7-9    38 white prepupae new    37 white prepupae 12hr    47 white prepupae 24hr    53 pupae 2d postWPP    37 pupae 3d postWPP    23 pupae 4d postWPP    15 adult male 01day    20 adult male 05day    25 adult male 30day    22 adult female 01day    32 adult female 05day    53 adult female 30day    56 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    61 Schneider line 2 Sg4    63 embryonic 1182-4H    35 embryonic GM2    26 embryonic Kc167    64 embryonic S1    106 embryonic S3    51 leg disc CME L1    66 wing disc CME-W2    62 wing disc ML-DmD8    58 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    127 wing disc ML-DmD21    135 wing disc ML-DmD32    61 haltere disc ML-DmD17-c3    68 eye-antennal disc ML-DmD11    65 antennal disc ML-DmD20-c5    73 mixed discs ML-DmD4-c1    57 CNS ML-DmBG1-c1    82 CNS ML-DmBG2-c2    75 tumorous blood cells mbn2    42 ovary fGS/OSS    73 ovary OSC    70 ovary OSS    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (61) Schneider line 2 Sg4  (63) embryonic 1182-4H  (35) embryonic GM2  26 embryonic Kc167  (64) embryonic S1  (106) embryonic S3  (51) leg disc CME L1  (66) wing disc CME-W2  (62) wing disc ML-DmD8  (58) wing disc ML-DmD9  (77) wing disc ML-DmD16-c3  (127) wing disc ML-DmD21  (135) wing disc ML-DmD32  (61) haltere disc ML-DmD17-c3  (68) eye-antennal disc ML-DmD11  (65) antennal disc ML-DmD20-c5  (73) mixed discs ML-DmD4-c1  (57) CNS ML-DmBG1-c1  (82) CNS ML-DmBG2-c2  (75) tumorous blood cells mbn2  (42) ovary fGS/OSS  (73) ovary OSC  (70) ovary OSS  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    61 Schneider line 2 Sg4    63 embryonic 1182-4H    35 embryonic GM2    26 embryonic Kc167    64 embryonic S1  106 embryonic S3    51 leg disc CME L1    66 wing disc CME-W2    62 wing disc ML-DmD8    58 wing disc ML-DmD9    77 wing disc ML-DmD16-c3  (127) wing disc ML-DmD21  (135) wing disc ML-DmD32    61 haltere disc ML-DmD17-c3    68 eye-antennal disc ML-DmD11    65 antennal disc ML-DmD20-c5    73 mixed discs ML-DmD4-c1    57 CNS ML-DmBG1-c1    82 CNS ML-DmBG2-c2    75 tumorous blood cells mbn2    42 ovary fGS/OSS    73 ovary OSC    70 ovary OSS    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    61 Schneider line 2 Sg4    63 embryonic 1182-4H    35 embryonic GM2    26 embryonic Kc167    64 embryonic S1    106 embryonic S3    51 leg disc CME L1    66 wing disc CME-W2    62 wing disc ML-DmD8    58 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    127 wing disc ML-DmD21    135 wing disc ML-DmD32    61 haltere disc ML-DmD17-c3    68 eye-antennal disc ML-DmD11    65 antennal disc ML-DmD20-c5    73 mixed discs ML-DmD4-c1    57 CNS ML-DmBG1-c1    82 CNS ML-DmBG2-c2    75 tumorous blood cells mbn2    42 ovary fGS/OSS    73 ovary OSC    70 ovary OSS    47 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    61 Schneider line 2 Sg4    63 embryonic 1182-4H    35 embryonic GM2    26 embryonic Kc167    64 embryonic S1    106 embryonic S3    51 leg disc CME L1    66 wing disc CME-W2    62 wing disc ML-DmD8    58 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    127 wing disc ML-DmD21    135 wing disc ML-DmD32    61 haltere disc ML-DmD17-c3    68 eye-antennal disc ML-DmD11    65 antennal disc ML-DmD20-c5    73 mixed discs ML-DmD4-c1    57 CNS ML-DmBG1-c1    82 CNS ML-DmBG2-c2    75 tumorous blood cells mbn2    42 ovary fGS/OSS    73 ovary OSC    70 ovary OSS    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (61) Schneider line 2 Sg4  (63) embryonic 1182-4H  (35) embryonic GM2  26 embryonic Kc167  (64) embryonic S1  (106) embryonic S3  (51) leg disc CME L1  (66) wing disc CME-W2  (62) wing disc ML-DmD8  (58) wing disc ML-DmD9  (77) wing disc ML-DmD16-c3  (127) wing disc ML-DmD21  (135) wing disc ML-DmD32  (61) haltere disc ML-DmD17-c3  (68) eye-antennal disc ML-DmD11  (65) antennal disc ML-DmD20-c5  (73) mixed discs ML-DmD4-c1  (57) CNS ML-DmBG1-c1  (82) CNS ML-DmBG2-c2  (75) tumorous blood cells mbn2  (42) ovary fGS/OSS  (73) ovary OSC  (70) ovary OSS  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    61 Schneider line 2 Sg4    63 embryonic 1182-4H    35 embryonic GM2    26 embryonic Kc167    64 embryonic S1  106 embryonic S3    51 leg disc CME L1    66 wing disc CME-W2    62 wing disc ML-DmD8    58 wing disc ML-DmD9    77 wing disc ML-DmD16-c3  127 wing disc ML-DmD21  135 wing disc ML-DmD32    61 haltere disc ML-DmD17-c3    68 eye-antennal disc ML-DmD11    65 antennal disc ML-DmD20-c5    73 mixed discs ML-DmD4-c1    57 CNS ML-DmBG1-c1    82 CNS ML-DmBG2-c2    75 tumorous blood cells mbn2    42 ovary fGS/OSS    73 ovary OSC    70 ovary OSS    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    61 Schneider line 2 Sg4    63 embryonic 1182-4H    35 embryonic GM2    26 embryonic Kc167    64 embryonic S1    106 embryonic S3    51 leg disc CME L1    66 wing disc CME-W2    62 wing disc ML-DmD8    58 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    127 wing disc ML-DmD21    135 wing disc ML-DmD32    61 haltere disc ML-DmD17-c3    68 eye-antennal disc ML-DmD11    65 antennal disc ML-DmD20-c5    73 mixed discs ML-DmD4-c1    57 CNS ML-DmBG1-c1    82 CNS ML-DmBG2-c2    75 tumorous blood cells mbn2    42 ovary fGS/OSS    73 ovary OSC    70 ovary OSS    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    29 Cadmium 50 mM 48 hrs, 4-day adult    38 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    78 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    67 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult  (31) Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3  (29) Cadmium 50 mM 48 hrs, 4-day adult  (38) Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  (78) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult  (30) Ethanol 2.5% 3 hrs, larvae L3  (42) Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (67) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (55) Paraquat 10 mM 48 hrs, 4-day adult  (29) Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    29 Cadmium 50 mM 48 hrs, 4-day adult    38 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    78 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    67 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    29 Cadmium 50 mM 48 hrs, 4-day adult    38 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    78 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    67 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    29 Cadmium 50 mM 48 hrs, 4-day adult    38 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    78 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    67 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult  31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3  29 Cadmium 50 mM 48 hrs, 4-day adult  (38) Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  (78) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult  30 Ethanol 2.5% 3 hrs, larvae L3  (42) Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (67) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (55) Paraquat 10 mM 48 hrs, 4-day adult  29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    29 Cadmium 50 mM 48 hrs, 4-day adult    38 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    78 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    67 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    14 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    29 Cadmium 50 mM 48 hrs, 4-day adult    38 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    78 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    18 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    67 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    23 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD34766 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010422 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3710-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for TfIIS Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Act5C.PU TfIISGD12773 lethal | recessive TfIISunspecified viable, with Scer\GAL4pnr-MD237 TfIISGD12773 Phenotype manifest in Allele wing TfIISunspecified
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of TfIIS Class Mutagen Stocks Known lesion TfIIS2 PM hybrid dysgenesis 2 -- TfIIS1 PM hybrid dysgenesis 1 -- TfIISunspecified PM hybrid dysgenesis 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of TfIIS Class Mutagen Stocks Known lesion TfIISGD12773 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes TfIISGL00353 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TfIISHMS01117 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TfIISKK101167 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TfIISdsRNA.cYa in vitro construct - RNAi 0 -- TfIISNIG.3710R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)64j (Ashburner et al., 1999, Iyengar et al., 1999, Roote, 2000.7.3, Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)A48 (Cai et al., 2001, Fossett et al., 1994) Df(2L)A72 (Ashburner et al., 1990, Fossett et al., 1994) Df(2L)A215 (Fossett et al., 1994) Df(2L)A220 (Fossett et al., 1994) Df(2L)A246 (Fossett et al., 1994) Df(2L)A263 (Furukawa et al., 1992) Df(2L)A264 (Fossett et al., 1994) Df(2L)A267 (Cai et al., 2001, Ashburner et al., 1990, Fossett et al., 1994) Df(2L)A337 (Fossett et al., 1994) Df(2L)A376 (Fossett et al., 1994) Df(2L)A400 (Fossett et al., 1994) Df(2L)Adh-nBR101 (Fossett et al., 1994) Df(2L)Adh-nBR102 (Fossett et al., 1994) Df(2L)Adh-nBR103 (Fossett et al., 1994) Df(2L)Adh-nBR105 (Fossett et al., 1994) Df(2L)Adh-nBR118 (Fossett et al., 1994) Df(2L)Adh-nBR120 (Fossett et al., 1994) Df(2L)Adh-nBR122 (Fossett et al., 1994) Df(2L)Adh-nBR125 (Fossett et al., 1994) Df(2L)Adh-nBR126 (Fossett et al., 1994) Df(2L)Adh-nBR128 (Fossett et al., 1994) Df(2L)Adh-nBR129 (Fossett et al., 1994) Df(2L)Adh-nBR130 (Fossett et al., 1994) Df(2L)Adh-nBR131 (Fossett et al., 1994) Df(2L)b80e4 (Ashburner et al., 1990) Df(2L)b84h50 (Furukawa et al., 1992) Df(2L)el20 (Ashburner et al., 1990) Df(2L)el28 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)Exel7061 (Ryder, 2004.4.26) Df(2L)Exel7062 (Ryder, 2004.4.26) Df(2L)fn5 (Furukawa et al., 1992) Df(2L)fn26 (Ashburner et al., 1990) Df(2L)fn27 (Ashburner et al., 1990) Df(2L)n78l3 (Ashburner et al., 1990) Df(2L)noc11 (Ashburner et al., 1990) Df(2L)osp29 (Cai et al., 2001, Furukawa et al., 1992) Df(2L)osp38 (Ashburner et al., 1990) Df(2L)Sco7 (Ashburner et al., 1990) Df(2L)TE35BC-3 (Cai et al., 2001, Ashburner et al., 1990) Df(2L)TE35BC-4 (Ashburner et al., 1990) Df(2L)TE35BC-24 (Iyengar et al., 1999) Df(2L)TE35BC-34 (Ashburner et al., 1990) Df(2L)TE35D-20 (Ashburner et al., 1990) Df(2L)TE35D-23 (Ashburner et al., 1990) In(2L)el18 (Ashburner et al., 1990) In(2LR)noc4LScorv9R (Cai et al., 2001) Tp(2;2)A446 (Fossett et al., 1994)
Not disrupted in	Df(2L)A47 (Fossett et al., 1994) Df(2L)A63 (Fossett et al., 1994) Df(2L)A178 (Fossett et al., 1994) Df(2L)A217 (Fossett et al., 1994) Df(2L)A245 (Fossett et al., 1994) Df(2L)A266 (Fossett et al., 1994) Df(2L)A379 (Fossett et al., 1994) Df(2L)A445 (Fossett et al., 1994) Df(2L)Adh-nBR100 (Fossett et al., 1994) Df(2L)Adh-nBR106 (Fossett et al., 1994, Roote, 1996.1.11, Roote, 1996.8) Df(2L)Adh-nBR107 (Fossett et al., 1994, Roote, 1996.1.11, Roote, 1996.8) Df(2L)Adh-nBR117 (Fossett et al., 1994) Df(2L)Adh-nBR119 (Fossett et al., 1994) Df(2L)Adh-nBR121 (Fossett et al., 1994) Df(2L)Adh-nBR127 (Fossett et al., 1994) Df(2L)ARR1 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)b84a3 (Furukawa et al., 1992) Df(2L)b84a7 (Furukawa et al., 1992) Df(2L)fn12 (Ashburner et al., 1990) Df(2L)H20 (Ashburner et al., 1990) Df(2L)PZ07130-mr1 (Roote, 2000.6.13) Df(2L)PZ07130-mr3 (Roote, 2000.6.13) Df(2L)PZ07130-mr8 (Roote, 2000.6.13) Df(2L)PZ07130-mr9 (Roote, 2000.6.13) Df(2L)r10 (Ashburner et al., 1990) Df(2L)RA5 (Ashburner et al., 1990) Df(2L)rd9 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)RM5 (Ashburner et al., 1990) Df(2L)RN2 (Ashburner et al., 1990) Df(2L)Scorv16 (Ashburner et al., 1990) Df(2L)Scorv18 (Ashburner et al., 1990) Df(2L)Scorv19 (Ashburner et al., 1990) Df(2L)Scorv25 (Ashburner et al., 1990) Df(2L)SS1 (Ashburner et al., 1990) Df(2L)TE35BC-7 (Furukawa et al., 1992) Df(2L)TE35BC-28 (Furukawa et al., 1992) Df(2L)TE35D-1 (Ashburner et al., 1990) Df(2L)TE35D-2 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)TE35D-3 (Ashburner et al., 1990) Df(2L)TE35D-4 (Ashburner et al., 1990) Df(2L)TE35D-6 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)TE35D-13 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)TE35D-14 (Ashburner et al., 1990) Df(2L)TE35D-15 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)TE35D-16 (Ashburner et al., 1990) Df(2L)TE35D-18 (Furukawa et al., 1992) Df(2L)TE35D-19 (Ashburner et al., 1990) Df(2L)TE35D-21 (Ashburner et al., 1990) Df(2L)TE35D-22 (Cai et al., 2001, Ashburner et al., 1990) In(2L)dpps22Ldppd36R (Ashburner et al., 1990) In(2LR)Scorv1-DV7 (Ashburner et al., 1990) T(2;3)Scorv7 (Ashburner et al., 1990)
Duplicated in	Dp(2;1)Scorv23 (Ashburner et al., 1990) Dp(2;2)GYL (Ashburner et al., 1990) Dp(2;2)TE35B-54L (Ashburner et al., 1990) Dp(2;3)osp3 (Ashburner et al., 1990) In(2L)C163.41LC158R (Ashburner et al., 1990) In(2L)Scorv17LC158R (Ashburner et al., 1990)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12773} NA P{KK101167} NA P{NIG.3710R} NA P{TRiP.GL00353} NA P{TRiP.HMS01117} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}vasKG01651 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR017923 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR001222, InterPro:IPR017890 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of DNA-dependent transcription, elongation inferred from electronic annotation with InterPro:IPR016492 (FlyBase Curators et al., 2004-) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with EMBL:AJ223472 (FlyBase, 1992-) regulation of transcription from RNA polymerase II promoter inferred from electronic annotation with InterPro:IPR016492 (FlyBase Curators et al., 2004-) transcription elongation from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1991.2.1)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity (Ashburner et al., 1999) inferred from sequence or structural similarity with EMBL:AJ223472 (FlyBase, 1992-) non-traceable author statement (Oh, 1993)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
TfIIS - Bx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TfIIS - CG17118 experimental knowledge based (Guruharsha et al., 2011) TfIIS - CG4854 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) TfIIS - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) TfIIS - MED6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions TfIIS allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60901 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010422 DroID - A comprehensive database of gene and protein interactions. • FBgn0010422 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34883
Orthologs
OrthoDB Orthologs (49) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VHKSN Dipteran inclusive ortholog search EOG6FFD9R Insect inclusive ortholog search EOG6RBPFR Arthropod inclusive ortholog search EOG62JM7G Metazoa inclusive ortholog search EOG62G5BT
Orthologs in Drosophila Species (EOG6VHKSN)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\TfIIS     Drosophila simulans   Dsim\GD21972 Y   Drosophila sechellia   Dsec\GM14545 Y   Drosophila erecta   Dere\GG24225 Y   Drosophila yakuba   Dyak\GE19417 Y   Drosophila ananassae   Dana\GF15561 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17632 Y   Drosophila persimilis   Dper\GL25727 Y   Drosophila willistoni   Dwil\GK18066 Y   Drosophila virilis   Dvir\TfIIS Y   Drosophila mojavensis   Dmoj\GI17330 Y   Drosophila grimshawi   Dgri\GH10818 Y
Orthologs in non-Drosophila Dipterans (EOG6FFD9R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001496   Anopheles gambiae Malaria mosquito  Agam\AGAP009035   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ802263   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ802264
Orthologs in non-Dipteran Insects (EOG6RBPFR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Ame.6676   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG022317   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11449   Atta cephalotes Leafcutter ant  Acep\ACEP21797   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22097   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20252   Linepithema humile Argentine ant  Lhum\LH10962   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17976   Solenopsis invicta Red fire ant  Sinv\SINV10592   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005512   Bombyx mori Silkmoth  Bmor\BGIBMGA006173   Pediculus humanus Human body louse  Phum\PHUM426350   Tribolium castaneum Red flour beetle  Tcas\TC014756
Orthologs in non-Insect Arthropods (EOG62JM7G)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\SEPSECS Y Ixodes scapularis Deer tick  Isca\ISCW000010 Y
Orthologs in non-Arthropod Metazoa (EOG62G5BT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\T24H10.1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020528gn Y Danio rerio Zebrafish  Drer\tcea1 Y Danio rerio Zebrafish  Drer\tcea2 Y Danio rerio Zebrafish  Drer\tcea3 Y Xenopus tropicalis Western clawed frog  Xtro\tcea1 Y Xenopus tropicalis Western clawed frog  Xtro\tcea3 Y Gallus gallus Domestic chicken  Ggal\TCEA1 Y Gallus gallus Domestic chicken  Ggal\TCEA2 Y Mus musculus House mouse  Mmus\Tcea1 Y Mus musculus House mouse  Mmus\Tcea2 Y Rattus norvegicus Norway rat  Rnor\Tcea2 Y Rattus norvegicus Norway rat  Rnor\Tcea3 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000008114 Y Rattus norvegicus Norway rat  Rnor\Tcea1 Y Homo sapiens Human  Hsap\ENSG00000187735 Y Homo sapiens Human  Hsap\TCEA3 Y Homo sapiens Human  Hsap\ENSG00000171703 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\ENSG00000187735     Hsap\TCEA3     Hsap\ENSG00000171703
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21972 Drosophila sechellia Dsec\GM14545 Drosophila erecta Dere\GG24225 Drosophila yakuba Dyak\GE19417 Drosophila ananassae Dana\GF15561 Drosophila pseudoobscura pseudoobscura Dpse\GA17632 Drosophila persimilis Dper\GL25727 Drosophila willistoni Dwil\GK18066 Drosophila virilis Dvir\TfIIS Drosophila mojavensis Dmoj\GI17330 Drosophila grimshawi Dgri\GH10818
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	3899 TfIIS2 bw1/CyO 35429 y1 sc* v1; P{TRiP.GL00353}attP2 34642 y1 sc* v1; P{TRiP.HMS01117}attP2
Kyoto	107259 TfIIS2 bw1/CyO 101537 TfIIS1 cn1 bw1 / CyO
VDRC	v103718 P{KK101167}VIE-260B v22979 w1118; P{GD12773}v22979 v22980 w1118; P{GD12773}v22980
Genomic Clones ( 2 )
	BACR03D18 BACR06I01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 87 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD34766
Other clones	DPiM_LD34766
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06459 GH16559 GM02906 GM03078 GM04006 GM09514 LD15545 LD18436 LD32510 LD33310 RE16305 RE27666 RE35323 RE55416 RE65844
Other clones	125888 153961 162193 CK01.56B.F11 CK01.74A.A12 CK01.74A.H11 CK01.121C.D8 Dmel_dig_008298_0654_3318 Dmel_neb_010298_0089_2991 EK045424 EK058330 EK058334 EK092948 EK248408 EK257458 EK265646 FGC015A08 FGM309E04 RP003016340 RP003035800 RP003037962 RP003048111 RP003051732 RP003076537 RP003080725 RP003090769 RP003094220 RP003095945 RP003097928 RP003110501 RP003131720 RP003145900 RP003150483 RP003162837 RP003166484 RP003179095 RP003191952 RP003196065 RP003217631 RP003221135 RP003241084 RP003250149 RP003270173 RP003286932 RP003297407 RP003304390 RP003307067 RP003323137 RP003324233 RP003330551 RP003346276 RP003385867 RP003402139 RP003409249 RP003446788 RP003451177 RP003463215 RP003473831 RP003476070 RP003484153 RP003487428 RP003492197 RP003514601 RP003557234 RP003575038 RP003577662 RP003617904 RP003640358 RP003663636 RP003676866
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010422 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34883
Antibody Information
	polyclonal (Marshall et al., 1990, Ni et al., 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: TfIIS CG3710 (Kodira, 1999.11)
Source for database merge of	Source for merge of: TfIIS l(2)35Cf (Ashburner et al., 1999)
Additional comments
Other Comments
	α-amanitin has an inhibitory effect on TfIIS-mediated transcript cleavage, but has no effect on elongation or pycophosphorolysis. (Chafin et al., 1995) TfIIS is necessary and sufficient to activate nascent transcript cleavage by RNA polymerase II during transcription of a dC-tailed template in vitro. (Guo and Price, 1993) 2 alleles are discussed but are not named. (Ashburner et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057619 NM_001273528 RefSeq (Proteins) • NP_476967 NP_001260457 DDBJ / EMBL / GenBank • AA440684 AA538819 AA695486 AA695587 AA697244 AA802194 AA951789 AA979056 AA990842 AAA92864 AAF44916 AAF53436 AAK93267 AC092238 AE003412 AGB92992 AW943246 AY051843 CAA37710 L26091 X53670 Entrez Gene - A searchable database of RefSeq genes. • 34883 UniProtKB/Swiss-Prot • P20232
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, TFIIS-type (IPR001222) Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type (IPR003617) Transcription elongation factor S-II, central domain (IPR003618) Transcription elongation factor, TFIIS (IPR006289) Transcription elongation factor, TFIIS-related (IPR016492) Transcription elongation factor S-IIM (IPR017890) Transcription factor IIS, N-terminal (IPR017923) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD34766
Linkouts
	BioGRID - A database of protein and genetic interactions • 60901 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0010422 DroID - A comprehensive database of gene and protein interactions. • FBgn0010422 DRSC - Results from RNAi screens. • FBgn0010422 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010422 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3710-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010422 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34883 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/tfiis.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34883 modMine - Data generated by the modENCODE project. • FBgn0010422
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	BG:DS00929.12 (Ashburner et al., 1999.10.12, Ashburner et al., 1999) br52 (Furukawa et al., 1992) CG3710 (Keleman et al., 2009.8.5, de Wit et al., 2005, Ni et al., 2010.12.1, Ni et al., 2011.7.19) DmS-II (Price, 1990.7.3, Chafin et al., 1995, Chen et al., 1996, Guo and Price, 1993, Kephart et al., 1992) DmSII   l35Cf (Fossett et al., 1994) l(2)35Cf (Roote, 2000.6.13, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Ashburner, 1995.4.11, Roote, 1996.1.11, Spradling et al., 1995, Roote, 1995.5.4, Ashburner et al., 1990, ) l(2)35cF (Iyengar et al., 1999) l(2)br52   RnpSII   TFIIS (Mizuguchi et al., 2001, Oh et al., 2001, Park et al., 2001, Reese et al., 2000, Oh et al., 1995, Kim and Lis, 2005, Nechaev et al., 2010, Jin et al., 2004, Ni et al., 2008, Ardehali et al., 2009) TfIIS (Dietzl et al., 2007, de Wit et al., 2005, Japanese National Institute of Genetics, 2012.5.21) TFIISA (Tinker et al., 1998) TFS-II (Mushegian et al., 1998)
Name Synonym	lethal(2)35Cf   RNA polymerase II elongation factor   transcription cleavage factor IIS (Nechaev et al., 2010)
Secondary FlyBase IDs
	FBgn0000474 FBgn0001982
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 7 ) Review  ( 2 ) Personal communication to FlyBase  ( 17 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 ) Conference report  ( 1 )
Recent research papers ( 2 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Gilchrist et al., 2012, Genes Dev. 26(9): 933--944 Regulating the regulators: the pervasive effects of Pol II pausing on stimulus-responsive gene networks. [FBrf0218258] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...