FlyBase Gene Report: Dmel\TfIIS

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

(UniProt, P20232)

Summary (Interactive Fly)

critical for efficient release of stalled RNA Pol II from intrinsic stop sites in promoter regions - promotes transcriptional elongation and decreases pausing

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\TfIIS or the JBrowse view of Dmel\TfIIS for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

TfIIS-RA

FBtr0080722

NM_057619

1401

313

TfIIS-RB

FBtr0332475

NM_001273528

1773

313

Additional Transcript Data and Comments

Reported size (kB)

1.4 (northern blot)

(Marshall et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

TfIIS-PA

FBpp0080281

34.3

313

9.58

NP_476967

AAF53436

TfIIS-PB

FBpp0304751

34.3

313

9.58

NP_001260457

AGB92992

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

313 aa isoforms: TfIIS-PA, TfIIS-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

313 (aa)

(Oh et al., 1995)

313 (aa); 34.3 (kD)

(Marshall et al., 1990)

36 (kD)

(Sluder et al., 1989)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TfIIS using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TfIIS_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

nucleic acid binding

inferred from electronic annotation with InterPro:IPR001222, InterPro:IPR006289

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

zinc ion binding

inferred from electronic annotation with InterPro:IPR001222, InterPro:IPR017890

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

regulation of transcription, DNA-templated

inferred from electronic annotation with InterPro:IPR006289

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

transcription elongation from RNA polymerase II promoter

inferred from sequence or structural similarity with SGD:S000003011

(FlyBase Curators, 2008--)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

nucleus

inferred from sequence or structural similarity with SGD:S000003011

(FlyBase Curators, 2008--)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

TfIIS transcripts are present in Kc cell RNA.

(Marshall et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\TfIIS in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\TfIIS

View exonic expression by tissue for Dmel\TfIIS

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010422

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010422

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3710-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3710

Flygut - An atlas of the Drosophila adult midgut

FBgn0010422

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\TfIIS

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of TfIIS

Transgenic constructs containing regulatory region of TfIIS

Deletions and Duplications ( 106 )

Disrupted in

Df(2L)64j

(Roote, 2000.7.3, Ashburner et al., 1999, Iyengar et al., 1999, Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)A48

(Cai et al., 2001, Fossett et al., 1994)

Df(2L)A72

(Fossett et al., 1994, Ashburner et al., 1990)

Df(2L)A215

(Fossett et al., 1994)

Df(2L)A220

(Fossett et al., 1994)

Df(2L)A246

(Fossett et al., 1994)

Df(2L)A263

(Furukawa et al., 1992)

Df(2L)A264

(Fossett et al., 1994)

Df(2L)A267

(Cai et al., 2001, Fossett et al., 1994, Ashburner et al., 1990)

Df(2L)A337

(Fossett et al., 1994)

Df(2L)A376

(Fossett et al., 1994)

Df(2L)A400

(Fossett et al., 1994)

Df(2L)Adh-nBR101

(Fossett et al., 1994)

Df(2L)Adh-nBR102

(Fossett et al., 1994)

Df(2L)Adh-nBR103

(Fossett et al., 1994)

Df(2L)Adh-nBR105

(Fossett et al., 1994)

Df(2L)Adh-nBR118

(Fossett et al., 1994)

Df(2L)Adh-nBR120

(Fossett et al., 1994)

Df(2L)Adh-nBR122

(Fossett et al., 1994)

Df(2L)Adh-nBR125

(Fossett et al., 1994)

Df(2L)Adh-nBR126

(Fossett et al., 1994)

Df(2L)Adh-nBR128

(Fossett et al., 1994)

Df(2L)Adh-nBR129

(Fossett et al., 1994)

Df(2L)Adh-nBR130

(Fossett et al., 1994)

Df(2L)Adh-nBR131

(Fossett et al., 1994)

Df(2L)b80e4

(Ashburner et al., 1990)

Df(2L)b84h50

(Furukawa et al., 1992)

Df(2L)el20

(Ashburner et al., 1990)

Df(2L)el28

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)Exel7061

(Ryder, 2004.4.26)

Df(2L)Exel7062

(Ryder, 2004.4.26)

Df(2L)fn5

(Furukawa et al., 1992)

Df(2L)fn26

(Ashburner et al., 1990)

Df(2L)fn27

(Ashburner et al., 1990)

Df(2L)n78l3

(Ashburner et al., 1990)

Df(2L)noc11

(Ashburner et al., 1990)

Df(2L)osp29

(Cai et al., 2001, Furukawa et al., 1992)

Df(2L)osp38

(Ashburner et al., 1990)

Df(2L)Sco7

(Ashburner et al., 1990)

Df(2L)TE35BC-3

(Cai et al., 2001, Ashburner et al., 1990)

Df(2L)TE35BC-4

(Ashburner et al., 1990)

Df(2L)TE35BC-24

(Iyengar et al., 1999)

Df(2L)TE35BC-34

(Ashburner et al., 1990)

Df(2L)TE35D-20

(Ashburner et al., 1990)

Df(2L)TE35D-23

(Ashburner et al., 1990)

In(2L)el18

(Ashburner et al., 1990)

In(2LR)noc^4LSco^rv9R

(Cai et al., 2001)

Tp(2;2)A446

(Fossett et al., 1994)

Not disrupted in

Df(2L)A47

(Fossett et al., 1994)

Df(2L)A63

(Fossett et al., 1994)

Df(2L)A178

(Fossett et al., 1994)

Df(2L)A217

(Fossett et al., 1994)

Df(2L)A245

(Fossett et al., 1994)

Df(2L)A266

(Fossett et al., 1994)

Df(2L)A379

(Fossett et al., 1994)

Df(2L)A445

(Fossett et al., 1994)

Df(2L)Adh-nBR100

(Fossett et al., 1994)

Df(2L)Adh-nBR106

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR107

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR117

(Fossett et al., 1994)

Df(2L)Adh-nBR119

(Fossett et al., 1994)

Df(2L)Adh-nBR121

(Fossett et al., 1994)

Df(2L)Adh-nBR127

(Fossett et al., 1994)

Df(2L)ARR1

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)b84a3

(Furukawa et al., 1992)

Df(2L)b84a7

(Furukawa et al., 1992)

Df(2L)fn12

(Ashburner et al., 1990)

Df(2L)H20

(Ashburner et al., 1990)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Ashburner et al., 1990)

Df(2L)RA5

(Ashburner et al., 1990)

Df(2L)rd9

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)RM5

(Ashburner et al., 1990)

Df(2L)RN2

(Ashburner et al., 1990)

Df(2L)Sco^rv16

(Ashburner et al., 1990)

Df(2L)Sco^rv18

(Ashburner et al., 1990)

Df(2L)Sco^rv19

(Ashburner et al., 1990)

Df(2L)Sco^rv25

(Ashburner et al., 1990)

Df(2L)SS1

(Ashburner et al., 1990)

Df(2L)TE35BC-7

(Furukawa et al., 1992)

Df(2L)TE35BC-28

(Furukawa et al., 1992)

Df(2L)TE35D-1

(Ashburner et al., 1990)

Df(2L)TE35D-2

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-3

(Ashburner et al., 1990)

Df(2L)TE35D-4

(Ashburner et al., 1990)

Df(2L)TE35D-6

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-13

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-14

(Ashburner et al., 1990)

Df(2L)TE35D-15

(Furukawa et al., 1992, Ashburner et al., 1990)

Df(2L)TE35D-16

(Ashburner et al., 1990)

Df(2L)TE35D-18

(Furukawa et al., 1992)

Df(2L)TE35D-19

(Ashburner et al., 1990)

Df(2L)TE35D-21

(Ashburner et al., 1990)

Df(2L)TE35D-22

(Cai et al., 2001, Ashburner et al., 1990)

In(2L)dpp^s22Ldpp^d36R

(Ashburner et al., 1990)

In(2LR)Sco^rv1-DV7

(Ashburner et al., 1990)

T(2;3)Sco^rv7

(Ashburner et al., 1990)

Duplicated in

Dp(2;1)Sco^rv23

(Ashburner et al., 1990)

Dp(2;2)GYL

(Ashburner et al., 1990)

Dp(2;2)TE35B-54^L

(Ashburner et al., 1990)

Dp(2;3)osp³

(Ashburner et al., 1990)

In(2L)C163.41^LC158^R

(Ashburner et al., 1990)

In(2L)Sco^rv17LC158^R

(Ashburner et al., 1990)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

TfIIS^GD12773

lethal | recessive

TfIIS^unspecified

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

TfIIS^GD12773

viable, with Scer\GAL4^Mef2.PR

TfIIS^GD12773

viable, with Scer\GAL4^pnr-MD237

TfIIS^GD12773

viable, with Scer\GAL4^tin.CΔ4

TfIIS^GD12773

Phenotype manifest in

Allele

wing

TfIIS^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TCEA1

14 of 15

Yes

Hsap\TCEA3

13 of 15

Yes

Hsap\TCEA2

12 of 15

Yes

Hsap\TCEANC

5 of 15

Yes

Hsap\MED26

1 of 15

Hsap\TCEANC2

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Tcea1

14 of 15

Yes

Mmus\Tcea2

12 of 15

Yes

Mmus\Tcea3

11 of 15

Yes

Mmus\Tceanc

5 of 15

Yes

Mmus\Med26

1 of 15

Mmus\Stra6l

1 of 15

Yes

Mmus\Tceanc2

1 of 15

Yes

Rattus norvegicus (Norway rat) (8)

Rnor\Tcea3

11 of 13

Yes

Rnor\Tcea2

10 of 13

Yes

Rnor\LOC498453

7 of 13

Yes

Rnor\Tcea1

6 of 13

Yes

Rnor\Tceanc

5 of 13

Yes

Rnor\Med26

1 of 13

Rnor\RGD1305807

1 of 13

Yes

Rnor\Tceanc2

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (6)

Xtro\tcea1

9 of 12

Yes

Xtro\LOC100490835

5 of 12

Yes

Xtro\tceanc

3 of 12

Yes

Xtro\LOC100490835

2 of 12

Yes

Xtro\med26

1 of 12

Xtro\tceanc2

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\tcea2

12 of 15

Yes

Drer\tcea1

11 of 15

Yes

Drer\tcea3

10 of 15

Yes

Drer\crsp7

1 of 15

Drer\si:dkey-33g10.1

1 of 15

Drer\tceanc2

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\T24H10.1

13 of 15

Yes

Cele\mdt-26

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (7)

Atha\TFIIS

8 of 9

Yes

Atha\AT4G18720

2 of 9

Yes

Atha\AT5G42325

2 of 9

Yes

Atha\AT2G25640

1 of 9

Atha\AT2G42725

1 of 9

Yes

Atha\AT5G11430

1 of 9

Atha\AT5G25520

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\DST1

14 of 15

Yes

Scer\BYE1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\tfs1

11 of 12

Yes

Spom\SPCC645.13

1 of 12

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190DF9 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\TfIIS

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17632

Drosophila persimilis

Dper\GL25727

Drosophila willistoni

Dwil\GK18066

Drosophila virilis

Dvir\TfIIS

Drosophila mojavensis

Dmoj\GI17330

Drosophila grimshawi

Dgri\GH10818

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509N5 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17016144

Glossina morsitans

Tsetse fly

Gmor\GMOY005723

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12635

Mayetiola destructor

Hessian fly

Mdes\Mdes001717

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001496

Anopheles darlingi

American malaria mosquito

Adar\ADAC005224

Anopheles gambiae

Malaria mosquito

Agam\AGAP009035

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004677

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004678

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0M91 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006173

Danaus plexippus

Monarch butterfly

Dple\DPOGS214124-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011383

Apis florea

Little honeybee

Aflo\2773576212

Apis mellifera

Western honey bee

Amel\Ame.6676

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13366

Bombus terrestris

Buff-tailed bumblebee

Bter\15212429

Linepithema humile

Argentine ant

Lhum\LH10962

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0001ca

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG022317

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06111

Tribolium castaneum

Red flour beetle

Tcas\TC014756

Pediculus humanus

Human body louse

Phum\PHUM426350

Rhodnius prolixus

Kissing bug

Rpro\RPRC005785

Cimex lectularius

Bed bug

Clec\CLEC005605

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005512

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11525

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0MH2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR009155

Ixodes scapularis

Black-legged tick

Isca\ISCW000010

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60591.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60590.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g12260

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_53771

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G10EJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tcea1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007759

Gallus gallus

Domestic chicken

Ggal\TCEA1

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000026702

Gallus gallus

Domestic chicken

Ggal\TCEA2

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

7 of 10

1 of 10

pps

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TCEA1; transcription elongation factor A1

14 of 15

601425

TCEA3; transcription elongation factor A3

13 of 15

604128

TCEA2; transcription elongation factor A2

12 of 15

604784

TCEANC; transcription elongation factor A N-terminal and central domain containing

5 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

TfIIS - CG17118

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014, Guruharsha et al., 2011)

TfIIS - CG4854

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014, Guruharsha et al., 2011)

TfIIS - Mef2

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

TfIIS - TfIIFβ

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60901

DroID - A comprehensive database of gene and protein interactions.

FBgn0010422

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34883

MIST (protein-protein) - An integrated Molecular Interaction Database

TfIIS

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B10-35B10

Sequence location

2L:15,058,919..15,060,757 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B10-35B10

Limits computationally determined from genome sequence between P{lacW}Su(H)^k07904 and P{EP}vig^EP812&P{PZ}stc⁰⁵⁴⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35B-35B

(determined by in situ hybridisation)

(Oh et al., 1995)

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-47.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Bloomington

3899

TfIIS² bw¹/CyO

35429

y¹ sc^* v¹ sev²¹; P{TRiP.GL00353}attP2

78713

y¹ sc^* v¹ sev²¹; P{TOE.GS02146}attP40

34642

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01117}attP2

FlyORF

F003063

M{UAS-TfIIS.ORF.3xHA.GW}ZH-86Fb

107259

101537

v103718

v22979

w¹¹¹⁸; P{GD12773}v22979

v22980

w¹¹¹⁸; P{GD12773}v22980

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (87)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD34766

Other clones

DPiM_LD34766

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010422

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010422

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34883

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ni et al., 2008, Marshall et al., 1990)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: TfIIS CG3710

(Kodira, 1999.11)

Source for database merge of

Source for merge of: TfIIS l(2)35Cf

(Ashburner et al., 1999)

Additional comments

Other Comments

α-amanitin has an inhibitory effect on TfIIS-mediated transcript cleavage, but has no effect on elongation or pycophosphorolysis.

(Chafin et al., 1995)

TfIIS is necessary and sufficient to activate nascent transcript cleavage by RNA polymerase II during transcription of a dC-tailed template in vitro.

(Guo and Price, 1993)

2 alleles are discussed but are not named.

(Ashburner et al., 1990)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 70 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34883

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P20232

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PFV9

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010422

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010422

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

TfIIS_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3710

Flygut - An atlas of the Drosophila adult midgut

FBgn0010422

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34883

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014756

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3710

modMine - A data warehouse for the modENCODE project

FBgn0010422

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Linkouts

BioGRID - A database of protein and genetic interactions.

60901

DPiM - Drosophila Protein interaction map

FBgn0010422

DroID - A comprehensive database of gene and protein interactions.

FBgn0010422

DRSC - Results frm RNAi screens

FBgn0010422

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010422

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3710-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010422

FlyMine - An integrated database for Drosophila genomics

FBgn0010422

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34883

MIST (protein-protein) - An integrated Molecular Interaction Database

TfIIS

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

BG:DS00929.12

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG3710

(Çiçek et al., 2016, Chen et al., 2012, Ni et al., 2011.7.19, Zhang et al., 2011, Ni et al., 2010.12.1, Zhang et al., 2010, Keleman et al., 2009.8.5, de Wit et al., 2005)

DmS-II

(Chen et al., 1996, Chafin et al., 1995, Guo and Price, 1993, Kephart et al., 1992, Price, 1990.7.3)

DmSII

RnpSII

TFIIS

(Nechaev et al., 2010, Ardehali et al., 2009, Ni et al., 2008, Kim and Lis, 2005, Jin et al., 2004, Mizuguchi et al., 2001, Oh et al., 2001, Park et al., 2001, Reese et al., 2000, Oh et al., 1995)

TFIISA

(Tinker et al., 1998)

TFS-II

(Mushegian et al., 1998)

TfIIS

(Transgenic RNAi Project members, 2017-, Handler et al., 2013, Handler et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Dietzl et al., 2007, de Wit et al., 2005)

br52

(Furukawa et al., 1992)

l(2)35Cf

(Roote, 2000.6.13, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Roote, 1996.1.11, Ashburner, 1995.4.11, Roote, 1995.5.4, Spradling et al., 1995, Ashburner et al., 1990)

l(2)35cF

(Iyengar et al., 1999)

l(2)br52

l35Cf

(Fossett et al., 1994)

Name Synonyms

RNA polymerase II elongation factor

lethal(2)35Cf

transcription cleavage factor IIS

(Nechaev et al., 2010)

Secondary FlyBase IDs

FBgn0000474
FBgn0001982

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (88)

Report Sections

Open Close