tyrosine kinase - Toll pathway - dorsal group - involved in signal transduction during induction of dorsal/ventral polarity during early embryogenesis - regulation of antimicrobial peptides.
Gene model reviewed during 5.47
501 (aa); 56 (kD)
Interacts (via Death domain) with tub (via Death domain). Interacts with Pellino (Pli).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pll using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\pll in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a decrease in cytokinetic index is seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.
Targeting of either tub or pll product to the plasma membrane by myristylation is sufficient to activate the signal transduction pathway that leads to translocation of the dl product. Activated Tl induces a localized recruitment of tub and pll proteins to the plasma membrane.
The embryonic regulatory pathway, comprising the gene products between spz and cact (Tl, tub and pll) but not the genes acting upstream or downstream (ea and dl), is involved in the induction of the Drs gene in adults.
Mutations that affect the synthesis of antimicrobial peptides dramatically lower the resistance of flies to infection.
Interaction of the pll kinase with the membrane associated tub protein is required for transduction of the dorsoventral signal in embryos. pll can function in signal transduction in the absence of tub and cannot act upstream of tub in the signaling pathway.
tub acts upstream of pll in the dorsoventral signal transduction pathway. The two-hybrid yeast interaction system demonstrates direct interaction of pll (bait) with tub (prey) to activate a reporter gene, Ecol\lacZ. Combinations pll dl and pll cact did not activate Ecol\lacZ expression.
pll is not essential for viability but lack of pll gene product decreases the chances of survival to adult stages. Different pll alleles interact with a different spectra of tub alleles. Double mutants of tub and pll show higher zygotic lethality than either single mutant.
Genetic and molecular characterization of pll shows that it encodes a protein kinase, predicted to have a serine and threonine specificity. The pll kinase represents a new member of the raf/mos family.
Double mutant combinations of pll with ea alleles demonstrate that spatial regulation of ea activity by localized zymogen activation is a key initial event in defining the polarity of the dorsal-ventral embryonic pattern.
Recessive dorsalizing mutants of the dorsal group gene pll have significantly reduced axial ratios in pupae.
Epistatic relationships exist between dorsalizing maternal effect mutations and "dppHin" alleles.