DWnt4, DWnt-4, Wnt-4
ligand - Wnt homolog - mesodermal - a Wingless antagonist - transposon dysregulation modulates Wnt4 signaling to control germline stem cell differentiation
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Gene model reviewed during 5.47
Gene model reviewed during 5.55
3.2 (northern blot)
389 (aa)
Palmitoleoylated by porcupine. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport Wnt4 to unique sites at the cell surface. Depalmitoleoylated by notum, leading to inhibit Wnt signaling pathway.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Wnt4 using the Feature Mapper tool.
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as ventral nerve cord anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as embryonic leading edge cell specific anlage
Wnt4 is expressed in visceral mesoderm at embryonic stage 11. At stage 13, after cardioblasts are specified, transcripts can be detected at a low level in cardioblasts and at high levels in certain posterior segment dorsal muscles. Wnt4 expression becomes stronger in the cardioblasts at stage 15, with higher expression levels in the posterior segments. Expression in the posterior three pairs of ostia progenitors increases dramatically just before ostia formation starts. Wnt4 expression increases progressively from anterior to posterior with the highest expression in the posterior-most pairs of ostia cells. At stage 16, Wnt4 expression is highest in the ostia cells and at low levels in non-ostia cardioblasts.
At embryonic stage 10, Wnt4 transcript is expressed in a continuous band along the dorsal side of the embryo, where heart progenitors are are specified. Wnt4 transcript is present in both the ectoderm and mesoderm at this stage. From embryonic stage 11, ectodermal expression becomes segmented, and is localized on the anterior side of en stripes; mesodermal expression is undetectable. Starting at stage 15, Wnt4 transcript is expressed myocardial cells, and stage 16 is more pronounced in the posterior region of the dorsal vessel. At the end of embryogenesis, Wnt4 transcript is expressed in mycardial and pericardial cells.
Wnt4 transcripts are first detected in stage 8 embryos where they appear at the anterior tip and in a segment polarity pattern along the trunk. 15 stripes are observed in the posteriormost cells of each parasegment. They are one cell wide and overlap the ventralmost wg cells. Shortly after, expression is seen in the foregut primordium and in dorsal patches that are thought to correspond to cells of the labral and ocular segment primordia. By stage 14, transcripts are visible in the CNS and the developing gut. CNS expression is not uniform but increases, progressively from anterior to posterior regions of the embryo. In the gut, transcripts are seen in the foregut, hindgut, and in two regions of the visceral mesoderm corresponding to parasegments 4 and 8. The latter two sites correspond to the primordia of the gastric caeca and the second midgut constriction.
A 2 h after pupal formation (APF) protein is detected in the apical cells as they migrate basally. At 16 h APF Wnt4-expressing cells have migrated between the terminal filaments and developing germaria. At 18 h APF these cells have moved between individual basal stalk clusters. Expression is detected in sheath, basal half of the terminal filament cells and regions I and II of the germarium in the adult.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Wnt4 in GBrowse 22-25
2-25
2-23.9
Maps close to wg.
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Wnt4 CG4698
Wnt4 is required for normal salivary gland migration in the embryo. It is required in the second phase of salivary gland migration, as the gland moves posteriorly within the embryo.
Wnt4 regulates the dorsoventral specificity of retinal projections.