FlyBase Gene Report: Dmel\Wnt4

WNTs -: WNTs are evolutionarily conserved secreted Cys-rich glycoproteins, defined by sequence homology to the original members of the family - Wnt1 in mouse and wingless (wg) in Drosophila. They are extracellular ligands for members of the Frizzled family of receptors as well as other receptors. (Adapted from PMID:23151663).

Protein Function (UniProtKB)

Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Acts downstream of homeotic complex genes in the visceral mesoderm and is required for embryonic segmentation. Also required for cell movement and FAK regulation during ovarian morphogenesis.

(UniProt, P40589)

Summary (Interactive Fly)

ligand - Wnt homolog - mesodermal - a Wingless antagonist - transposon dysregulation modulates Wnt4 signaling to control germline stem cell differentiation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Wnt4 or the JBrowse view of Dmel\Wnt4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Wnt4-RA

FBtr0089291

NM_057624

3367

539

Wnt4-RB

FBtr0334799

NM_001273258

2938

539

Additional Transcript Data and Comments

Reported size (kB)

3.2 (northern blot)

(Graba et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Wnt4-PA

FBpp0088345

58.7

539

9.29

NP_476972

AAF52499

Wnt4-PB

FBpp0306845

58.7

539

9.29

NP_001260187

AGB92723

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

539 aa isoforms: Wnt4-PA, Wnt4-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

389 (aa)

(Graba et al., 1995)

Comments

External Data

Post Translational Modification

Palmitoleoylated by porcupine. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport Wnt4 to unique sites at the cell surface. Depalmitoleoylated by notum, leading to inhibit Wnt signaling pathway.

(UniProt, P40589)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Wnt4 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Wnt4_1

Linkouts

Gene Ontology (20 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

frizzled binding

inferred from physical interaction with FLYBASE:fz; FB:FBgn0001085, FLYBASE:fz2; FB:FBgn0016797, FLYBASE:fz4; FB:FBgn0027342

(Wu and Nusse, 2002)

protein binding

inferred from physical interaction with FLYBASE:fj; FB:FBgn0000658

(Lim et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

frizzled binding

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(assigned by GO_Central )

(Gaudet et al., 2011)

signaling receptor binding

inferred from sequence or structural similarity with FLYBASE:wg; FB:FBgn0284084

(FlyBase Curators, 2008--)

Biological Process (14 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

cell migration

inferred from mutant phenotype

(assigned by UniProt )

(Cohen et al., 2002)

establishment of planar polarity

inferred from genetic interaction with FLYBASE:fj; FB:FBgn0000658

(Lim et al., 2005)

female gonad development

inferred from mutant phenotype

(assigned by UniProt )

(Cohen et al., 2002)

germ-line stem-cell niche homeostasis

inferred from mutant phenotype

(Wang et al., 2015)

motor neuron axon guidance

inferred from mutant phenotype

(Inaki et al., 2007)

neural retina development

inferred from mutant phenotype

(Sato et al., 2006)

non-canonical Wnt signaling pathway

inferred from mutant phenotype

(Sato et al., 2006)

regulation of hemocyte proliferation

inferred from mutant phenotype

(Sinenko et al., 2010)

retinal ganglion cell axon guidance

inferred from mutant phenotype

(Sato et al., 2006)

salivary gland morphogenesis

inferred from mutant phenotype

(Harris and Beckendorf, 2007)

synaptic target inhibition

inferred from mutant phenotype

(Inaki et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

cell fate commitment

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(assigned by GO_Central )

(Gaudet et al., 2011)

neuron differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(assigned by GO_Central )

(Gaudet et al., 2011)

Wnt signaling pathway

inferred from sequence or structural similarity with FLYBASE:wg; FB:FBgn0284084

(FlyBase Curators, 2008--, Graba et al., 1995)

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

cell surface

inferred from direct assay

(Sato et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

extracellular region

inferred from sequence model

(Graba et al., 1995)

non-traceable author statement

(assigned by UniProt )

(Cohen et al., 2002)

inferred from sequence or structural similarity with FLYBASE:wg; FB:FBgn0284084

(FlyBase Curators, 2008--)

extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000246517

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Graba et al., 1995)

presumptive embryonic/larval central nervous system

(Graba et al., 1995)

ectoderm

(Graba et al., 1995)

embryonic stage 16

gastric caecum

(Graba et al., 1995)

embryonic midgut constriction 2

(Graba et al., 1995)

embryonic/larval heart

(Tauc et al., 2012)

ventral longitudinal muscle 1 | restricted

(Inaki et al., 2007)

A1-7 ventral longitudinal muscle 2 | restricted

(Inaki et al., 2007)

embryonic stage 8 -- 11

organism | segment polarity expression pattern

(Graba et al., 1995)

embryonic stage 14

embryonic/larval foregut

(Graba et al., 1995)

parasegment 4 of visceral mesoderm

(Graba et al., 1995)

parasegment 8 of visceral mesoderm

(Graba et al., 1995)

embryonic/larval hindgut

(Graba et al., 1995)

embryonic stage 11

organism | segmentally repeated

(Sato et al., 1999)

parasegment 4 of visceral mesoderm

(Sato et al., 1999)

ventral nerve cord

(Sato et al., 1999)

head segment | segmentally repeated

(Buratovich et al., 2000)

epidermis | segmentally repeated | ventral

(Buratovich et al., 2000)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

foregut anlage

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic anal pad

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic epipharynx

(Fisher et al., 2012)

embryonic esophagus

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic large intestine

(Fisher et al., 2012)

embryonic proventriculus

(Fisher et al., 2012)

embryonic rectum

(Fisher et al., 2012)

embryonic small intestine

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

gastric caecum

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

embryonic inner optic lobe primordium

(Fisher et al., 2012)

embryonic stage 10

cardiogenic mesoderm | dorsal | uniform

(Tauc et al., 2012)

embryonic stage 11 -- 13

dorsal ectoderm | segmentally repeated

(Tauc et al., 2012)

embryonic stage 15 -- 17

embryonic cardioblast

(Tauc et al., 2012)

embryonic stage 16 -- 17

embryonic/larval pericardial cell

(Tauc et al., 2012)

Additional Descriptive Data

At embryonic stage 10, Wnt4 transcript is expressed in a continuous band along the dorsal side of the embryo, where heart progenitors are are specified. Wnt4 transcript is present in both the ectoderm and mesoderm at this stage. From embryonic stage 11, ectodermal expression becomes segmented, and is localized on the anterior side of en stripes; mesodermal expression is undetectable. Starting at stage 15, Wnt4 transcript is expressed myocardial cells, and stage 16 is more pronounced in the posterior region of the dorsal vessel. At the end of embryogenesis, Wnt4 transcript is expressed in mycardial and pericardial cells.

(Tauc et al., 2012)

Wnt4 transcripts are first detected in stage 8 embryos where they appear at the anterior tip and in a segment polarity pattern along the trunk. 15 stripes are observed in the posteriormost cells of each parasegment. They are one cell wide and overlap the ventralmost wg cells. Shortly after, expression is seen in the foregut primordium and in dorsal patches that are thought to correspond to cells of the labral and ocular segment primordia. By stage 14, transcripts are visible in the CNS and the developing gut. CNS expression is not uniform but increases, progressively from anterior to posterior regions of the embryo. In the gut, transcripts are seen in the foregut, hindgut, and in two regions of the visceral mesoderm corresponding to parasegments 4 and 8. The latter two sites correspond to the primordia of the gastric caeca and the second midgut constriction.

(Graba et al., 1995)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

germarium region 2

(Cohen et al., 2002)

terminal filament basal cell

(Cohen et al., 2002)

germarium region 1

(Cohen et al., 2002)

epithelial sheath

(Cohen et al., 2002)

pupal stage

epithelial sheath

(Cohen et al., 2002)

Additional Descriptive Data

A 2 h after pupal formation (APF) protein is detected in the apical cells as they migrate basally. At 16 h APF Wnt4-expressing cells have migrated between the terminal filaments and developing germaria. At 18 h APF these cells have moved between individual basal stalk clusters. Expression is detected in sheath, basal half of the terminal filament cells and regions I and II of the germarium in the adult.

(Cohen et al., 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

cell surface

inferred from direct assay

(Sato et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Wnt4 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Wnt4

View exonic expression by tissue for Dmel\Wnt4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010453

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010453

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4698-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4698

Flygut - An atlas of the Drosophila adult midgut

FBgn0010453

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Wnt4

Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Wnt4

Transgenic constructs containing regulatory region of Wnt4

Deletions and Duplications ( 7 )

Disrupted in

Df(2L)ade3

(Cohen et al., 2002)

Df(2L)BSC226

(Christensen and Cook, 2006.12.5)

Df(2L)BSC291

(Christensen and Cook, 2007.5.8)

Df(2L)BSC324

(Kahsai and Cook, 2018, Christensen et al., 2007.10.29)

Df(2L)DE

(Cohen et al., 2002)

Df(2L)Exel6017

(Ryder, 2004.4.26)

Df(2L)RF

(Cohen et al., 2002)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(2L)BSC324)

Wnt4^C1

lethal | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

lethal - all die before end of P-stage, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cSa

partially lethal

Wnt4^K2

partially lethal - majority die | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Wnt4^GD5347

viable, with Scer\GAL4^Mef2.PR

Wnt4^GD5347

viable, with Scer\GAL4^pnr-MD237

Wnt4^GD5347

viable, with Scer\GAL4^tin.CΔ4

Wnt4^GD5347

Sterility

Allele

sterile | recessive

Other Phenotypes

Allele

cell migration defective

Wnt4^EMS23

cell shape defective

Wnt4^EMS23

neuroanatomy defective

Wnt4^P23

neuroanatomy defective, with Scer\GAL4^NP6099

Wnt4^UAS.WT

neuroanatomy defective (with Wnt4^C1)

Wnt4^EMS23

neuroanatomy defective (with Wnt4^EMS23)

neuroanatomy defective (with Wnt4^P23)

Wnt4^EMS23

pain response defective | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Wnt4^GD5347

planar polarity defective, with Scer\GAL4^dpp.blk1

Wnt4^UAS.cGa

planar polarity defective, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

planar polarity defective (with Df(2L)spd^j2)

Wnt4^K2

planar polarity defective | somatic clone, with Scer\GAL4^Act5C.PP

Wnt4^UAS.cGa

some die during larval stage | recessive

some die during pharate adult stage, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cSa

visible, with Scer\GAL4^dpp.blk1

Wnt4^UAS.cGa

visible, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

visible | heat sensitive (with Df(2L)spd^j2)

Wnt4^K2

Phenotype manifest in

Allele

alula | ectopic | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

anterior crossvein, with Scer\GAL4^dpp.blk1

Wnt4^UAS.cGa

anterior crossvein, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

denticle | ectopic, with Scer\GAL4^arm.PS

Wnt4^UAS.cSa

denticle | ectopic, with Scer\GAL4^da.G32

Wnt4^UAS.cGa

denticle belt | ectopic, with Scer\GAL4^da.G32

Wnt4^UAS.cGa

dorsal denticle, with Scer\GAL4^da.G32

Wnt4^UAS.cGa

dorsocentral bristle | supernumerary, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

egg chamber

Wnt4^unspecified

embryonic/first instar larval cuticle, with Scer\GAL4^da.G32

Wnt4^UAS.cGa

embryonic/larval heart, with Scer\GAL4^HCH.Hand

Wnt4^KK102348

embryonic/larval heart (with Df(2L)BSC226)

Wnt4^EMS23

embryonic/larval heart | embryonic stage 14

Wnt4^EMS23

embryonic/larval optic stalk (with Wnt4^C1)

Wnt4^EMS23

embryonic/larval optic stalk (with Wnt4^EMS23)

Wnt4^C1

embryonic/larval ostium

Wnt4^EMS23

embryonic/larval ostium, with Scer\GAL4^HCH.Hand

Wnt4^KK102348

embryonic/larval ostium (with Df(2L)BSC226)

Wnt4^EMS23

embryonic cardioblast, with Scer\GAL4^HCH.Hand

Wnt4^KK102348

embryonic cardioblast (with Df(2L)BSC226)

Wnt4^EMS23

embryonic cardioblast | embryonic stage 14

Wnt4^EMS23

embryonic epidermis | dorsal, with Scer\GAL4^da.G32

Wnt4^UAS.cGa

embryonic somatic muscle

Wnt4^EMS23

embryonic ventral epidermis, with Scer\GAL4^arm.PS

Wnt4^UAS.cSa

eye equator | heat sensitive (with Df(2L)spd^j2)

Wnt4^K2

eye photoreceptor cell

Wnt4^P23

eye photoreceptor cell, with Scer\GAL4^NP6099

Wnt4^UAS.WT

eye photoreceptor cell (with Df(2L)spd^j2)

Wnt4^K2

eye photoreceptor cell (with Wnt4^C1)

Wnt4^EMS23

eye photoreceptor cell (with Wnt4^EMS23)

eye photoreceptor cell (with Wnt4^P23)

Wnt4^EMS23

eye ventral compartment (with Df(2L)spd^j2)

Wnt4^K2

germarium

Wnt4^unspecified

haltere | ectopic | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

lamina

Wnt4^P23

lamina, with Scer\GAL4^NP6099

Wnt4^UAS.WT

lamina (with Wnt4^C1)

Wnt4^EMS23

lamina (with Wnt4^EMS23)

lamina (with Wnt4^P23)

Wnt4^EMS23

mesothoracic tergum | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

neuron

Wnt4^P23

neuron, with Scer\GAL4^NP6099

Wnt4^UAS.WT

neuron (with Wnt4^C1)

Wnt4^EMS23

neuron (with Wnt4^EMS23)

neuron (with Wnt4^P23)

Wnt4^EMS23

ommatidium (with Df(2L)spd^j2)

Wnt4^K2

ovarian sheath

Wnt4^unspecified

ovariole

Wnt4^C1

ovary

Wnt4^unspecified

posterior postalar bristle | supernumerary, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

presumptive embryonic salivary gland

Wnt4^EMS23

presumptive embryonic salivary gland, with Scer\GAL4^bap.3

Wnt4^UAS.cGa

scutellar bristle | supernumerary, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

scutellum | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

sternopleural bristle | supernumerary, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

wing, with Scer\GAL4^ptc-559.1

wing (with Df(2L)spd^j2)

Wnt4^K2

wing | ectopic | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

wing disc | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

wing hair, with Scer\GAL4^dpp.blk1

Wnt4^UAS.cGa

wing hair, with Scer\GAL4^en-e16E

Wnt4^UAS.cGa

wing hair, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

wing hair | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PP

Wnt4^UAS.cGa

wing margin bristle | ectopic | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

wing sensillum | ectopic | heat sensitive, with Scer\GAL4^ptc-559.1

Wnt4^UAS.cGa

wing vein | ectopic (with Df(2L)spd^j2)

Wnt4^K2

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (19)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\WNT9B

7 of 15

Yes

Hsap\WNT9A

5 of 15

Yes

Hsap\WNT11

4 of 15

Yes

Hsap\WNT1

3 of 15

Hsap\WNT2B

3 of 15

Hsap\WNT2

3 of 15

Yes

Hsap\WNT16

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (19)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Wnt9b

7 of 15

Yes

Mmus\Wnt9a

5 of 15

Yes

Mmus\Wnt11

4 of 15

Yes

Mmus\Wnt1

3 of 15

Mmus\Wnt2b

3 of 15

Mmus\Wnt2

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Rattus norvegicus (Norway rat) (19)

Rnor\Wnt9b

7 of 13

Yes

Rnor\Wnt11

4 of 13

Yes

Rnor\Wnt2b

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Rnor\Wnt16

2 of 13

Yes

Rnor\Wnt1

1 of 13

Rnor\Wnt2

1 of 13

Yes

Rnor\Wnt4

1 of 13

Rnor\Wnt10a

1 of 13

Rnor\Wnt10b

1 of 13

Xenopus tropicalis (Western clawed frog) (18)

Xtro\wnt9b

4 of 12

Yes

Xtro\wnt1

3 of 12

Xtro\wnt9b.2

3 of 12

Yes

Xtro\wnt11

3 of 12

Yes

Xtro\wnt2b

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (25)

Drer\wnt9b

6 of 15

Yes

Drer\wnt11

4 of 15

Yes

Drer\wnt11r

4 of 15

Yes

Drer\wnt1

3 of 15

Drer\wnt2

3 of 15

Yes

Drer\wnt9a

3 of 15

Yes

Drer\LOC568110

2 of 15

Drer\wnt2ba

2 of 15

Yes

Drer\wnt2bb

2 of 15

Yes

Drer\wnt3

2 of 15

Yes

Drer\wnt4a

2 of 15

Drer\wnt4b

2 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\lin-44

3 of 15

Yes

Cele\mom-2

3 of 15

Yes

Cele\cwn-1

2 of 15

Cele\cwn-2

2 of 15

Cele\egl-20

2 of 15

Arabidopsis thaliana (thale-cress) (7)

Atha\AT3G30220

1 of 9

Yes

Atha\AT3G32160

1 of 9

Yes

Atha\AT4G07740

1 of 9

Yes

Atha\AT4G11300

1 of 9

Yes

Atha\AT4G23530

1 of 9

Yes

Atha\AT5G33898

1 of 9

Yes

Atha\AT5G35603

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091907QX )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Wnt4

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25881

Drosophila persimilis

Dper\GL19231

Drosophila willistoni

Dwil\GK24728

Drosophila virilis

Dvir\GJ17616

Drosophila mojavensis

Dmoj\GI17793

Drosophila grimshawi

Dgri\GH13151

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506CU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17005375

Musca domestica

House fly

Mdoa\17005449

Glossina morsitans

Tsetse fly

Gmor\GMOY006806

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10356

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000632

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010739

Anopheles darlingi

American malaria mosquito

Adar\ADAC005728

Anopheles gambiae

Malaria mosquito

Agam\AGAP009735

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005836

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0F8R )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006145

Danaus plexippus

Monarch butterfly

Dple\DPOGS214086-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011441

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00568

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00567

Tribolium castaneum

Red flour beetle

Tcas\Wnt9

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03611

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0OFF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt16

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt9

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-WntA_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt10

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wnt3

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002145

Ciona intestinalis

Vase tunicate

Cint\LOC778938

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000000407

Ciona intestinalis

Vase tunicate

Cint\LOC778723

Ciona intestinalis

Vase tunicate

Cint\LOC778792

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009599

Gallus gallus

Domestic chicken

Ggal\WNT7B

Gallus gallus

Domestic chicken

Ggal\Gga.47682

Gallus gallus

Domestic chicken

Ggal\WNT3A

Gallus gallus

Domestic chicken

Ggal\WNT4

Gallus gallus

Domestic chicken

Ggal\WNT2B

Gallus gallus

Domestic chicken

Ggal\WNT9B

Gallus gallus

Domestic chicken

Ggal\WNT9a

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028069

Gallus gallus

Domestic chicken

Ggal\WNT11

Gallus gallus

Domestic chicken

Ggal\WNT7A

Gallus gallus

Domestic chicken

Ggal\WNT5A

Gallus gallus

Domestic chicken

Ggal\WNT5B

Gallus gallus

Domestic chicken

Ggal\WNT3

Gallus gallus

Domestic chicken

Ggal\WNT6

Gallus gallus

Domestic chicken

Ggal\WNT10A

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (6)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

3 of 10

Wnt2

3 of 10

Wnt6

3 of 10

wntD

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Wnt4^C1

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Wnt4^EMS23

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

WNT9B; Wnt family member 9B

7 of 15

602864

WNT9A; Wnt family member 9A

5 of 15

602863

WNT11; Wnt family member 11

4 of 15

603699

WNT1; Wnt family member 1

3 of 15

164820

osteogenesis imperfecta type 15

WNT2B; Wnt family member 2B

3 of 15

601968

DIARRHEA 9; DIAR9

WNT2; Wnt family member 2

3 of 15

147870

WNT16; Wnt family member 16

3 of 15

606267

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Wnt4 - Ror

anti tag coimmunoprecipitation, anti tag western blot

(Ripp et al., 2018)

Wnt4 - fj

pull down, anti tag western blot

(Lim et al., 2005)

Wnt4 - fz

affinity technology, enzyme linked immunosorbent assay, inferred by author

(Wu and Nusse, 2002)

Wnt4 - fz2

affinity technology, enzyme linked immunosorbent assay, inferred by author

(Wu and Nusse, 2002)

Wnt4 - fz4

affinity technology, enzyme linked immunosorbent assay, inferred by author

(Wu and Nusse, 2002)

Wnt4 - otk

anti tag coimmunoprecipitation, western blot

(Peradziryi et al., 2011)

Wnt4 - por

anti tag coimmunoprecipitation, anti tag western blot

(Herr and Basler, 2012)

Wnt4 - wls

anti bait coimmunoprecipitation, anti tag western blot

(Herr and Basler, 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Wnt4

enhanceable

hep

(Sato et al., 2006)

Wnt4

enhanceable

Wnt5

(Harris and Beckendorf, 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Wnt4

(Lim et al., 2005)

suppressible

Wnt4

(Lim et al., 2005)

hep

enhanceable

Wnt4

(Sato et al., 2006)

suppressible

Wnt4

(Buratovich et al., 2000)

suppressible

Wnt4

(Gieseler et al., 2001)

Wnt5

enhanceable

Wnt4

(Harris and Beckendorf, 2007)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60153

DroID - A comprehensive database of gene and protein interactions.

FBgn0010453

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34007

MIST (genetic) - An integrated Molecular Interaction Database

Wnt4

MIST (protein-protein) - An integrated Molecular Interaction Database

Wnt4

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-25

Cytogenetic map

27E7-27E8

Sequence location

2L:7,255,419..7,277,171 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

27E7-27E8

Limits computationally determined from genome sequence between P{PZ}snRNP70K⁰²¹⁰⁷ and P{PZ}wg⁰²⁶⁵⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

27F-28A

(determined by in situ hybridisation)

(Gieseler et al., 1995)

Experimentally Determined Recombination Data

Location

2-25

(FlyBase, 2016)

2-23.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Maps close to wg.

(Graba et al., 1995)

Stocks and Reagents

Stocks (17)

Bloomington

6651

w¹¹¹⁸; Wnt4^C1/CyO

29442

y¹ v¹; P{TRiP.JF03378}attP2

6650

Wnt4^EMS23 bw¹/CyO, P{HB-lacZ}GS1

36982

y¹ w^*; Mi{MIC}Wnt4^MI03717/SM6a

20162

y¹ w^67c23; P{EPgy2}Wnt4^EY06939/CyO

67449

y¹ w^*; Mi{Trojan-GAL4.2}Wnt4^{MI03717-TG4.2}

FlyORF

F001112

M{UAS-Wnt4.ORF.3xHA}ZH-86Fb

Kyoto

108974

Wnt4^EMS23 bw¹/CyO, P{HB-lacZ}GS1

VDRC

v104671

P{KK102348}VIE-260B

v38010

w¹¹¹⁸; P{GD5347}v38010

v38011

w¹¹¹⁸; P{GD5347}v38011/CyO

v341833

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00986}attP40/CyO-GFP

More stocks available...

Genomic Clones (29)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (189)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

RE26454

Other clones

FI03360

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010453

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE42237

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010453

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34007

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Cohen et al., 2002)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Wnt4 CG4698

(Deng, 1999.11)

Source for database merge of

Additional comments

Other Comments

Wnt4 is required for normal salivary gland migration in the embryo. It is required in the second phase of salivary gland migration, as the gland moves posteriorly within the embryo.

(Harris and Beckendorf, 2007)

Wnt4 regulates the dorsoventral specificity of retinal projections.

(Sato et al., 2006)

Wnt4 is required for cell movement and Fak56D regulation during ovarian morphogenesis. Wnt4 facilitates cell motility through regulation of focal adhesions.

(Cohen et al., 2002)

Wnt4 and wg produce distinct responses in cells of the dorsal embryonic epidermis. wg acts independently of hh signaling, while Wnt4 requires hh to elicit its effects.

(Buratovich et al., 2000)

Wnt4 has the capacity to antagonise wg signalling in the embryonic ventral epidermis.

(Gieseler et al., 1999)

wg and Wnt4 have partially overlapping embryonic expression patterns. Expression is regulated by HOM-C genes in the visceral mesoderm and segment polarity genes in the ectoderm. wg and Wnt4 achieve opposite, but complementary functions in intrasegmental cell patterning of the embryonic ectoderm.

(Gieseler et al., 1995)

Wnt4 has been characterised, Wnt4 gene product has a segment polarity-like expression pattern in the ectoderm and is controlled by homeotic complex (HOM-C) genes in the visceral mesoderm.

(Graba et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34007

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P40589

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

D2NUH8

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010453

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010453

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Wnt4_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4698

Flygut - An atlas of the Drosophila adult midgut

FBgn0010453

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34007

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013708

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4698

modMine - A data warehouse for the modENCODE project

FBgn0010453

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P40589

Linkouts

BioGRID - A database of protein and genetic interactions.

60153

DroID - A comprehensive database of gene and protein interactions.

FBgn0010453

DRSC - Results frm RNAi screens

FBgn0010453

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010453

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4698-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010453

FlyMine - An integrated database for Drosophila genomics

FBgn0010453

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34007

MIST (genetic) - An integrated Molecular Interaction Database

Wnt4

MIST (protein-protein) - An integrated Molecular Interaction Database

Wnt4

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG4698

(McEachern et al., 2014, Schertel et al., 2013, Keleman et al., 2009.8.5, Perkins et al., 2009)

DWnt-4

(Chen et al., 2008, Pandur et al., 2002, Cohen and Wilder, 2001, Cohen et al., 2001, Logan et al., 2001, Buratovich et al., 2000, Cohen et al., 2000, Wilder and Wallace, 2000, Wilder and Wallace, 2000, Buratovich and Wilder, 1999, Gieseler et al., 1999, Wilder et al., 1999, Buratovich and Wilder, 1998, Perrimon and Nusse, 1998, Cadigan and Nusse, 1997, Graba et al., 1997, Wilder et al., 1997, Wilder et al., 1997, Graba et al., 1995)

Dm DWnt4

(Konikoff et al., 2010)

Dwnt-4

(Swarup and Verheyen, 2012)

Dwnt4

(Perochon et al., 2018, Joshi et al., 2007, Bovolenta et al., 2006, Terry et al., 2006, Huang and Klein, 2004, Cadigan et al., 2002, Taylor, 2000)

WNT4

(Harris et al., 2016)

Wnt

(Blair, 1999)

Wnt-4

(Bajpai et al., 2004, Jockusch and Ober, 2000, Wehrli and Tomlinson, 1998)

Wnt4

(Wu et al., 2019, Cho et al., 2018, Jankovics et al., 2018, Ripp et al., 2018, Tian et al., 2018, Waghmare and Page-McCaw, 2018, Hessinger et al., 2017, Transgenic RNAi Project members, 2017-, Chen et al., 2016, Dominguez et al., 2016, Gene Disruption Project members, 2016-, Mottier-Pavie et al., 2016, Hamada-Kawaguchi et al., 2014, Kerr et al., 2014, Linnemannstöns et al., 2014, Toshima et al., 2014, Schertel et al., 2013, Sopko and Perrimon, 2013, Webber et al., 2013, Wu et al., 2013, Herr and Basler, 2012, Japanese National Institute of Genetics, 2012.5.21, Kuroda et al., 2012, Lahaye et al., 2012, Nose, 2012, Cherbas et al., 2011, Peradziryi et al., 2011, Biehs et al., 2010, Bina et al., 2010, Inaki et al., 2010, Kong et al., 2010, Chen et al., 2008, Christensen and Cook, 2007.5.8, Christensen et al., 2007.10.29, Harris and Beckendorf, 2007, Inaki et al., 2007, Zeitlinger et al., 2007, Zeitouni et al., 2007, Casal et al., 2006, Christensen and Cook, 2006.12.5, Ma et al., 2006, Pearson et al., 2005)

anon-EST:Liang-2.4

clone 2.4

(Liang and Biggin, 1998)

dWnt4

(Humphries and Mlodzik, 2018)

wnt-4

(Liang and Biggin, 1998)

wnt4

(Ewen-Campen et al., 2017, Khan et al., 2013, Sinenko et al., 2010, Srahna et al., 2006, Veeman et al., 2003, Bhat, 1999, Bhat, 1998)

Name Synonyms

Wnt oncogene analog 4

(McEachern et al., 2014)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (161)

Report Sections

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