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Gene Dmel\Wnt4

General Information
Symbol	Dmel\Wnt4	Species	D. melanogaster
Name	Wnt oncogene analog 4	Annotation symbol	CG4698
Feature type	protein_coding_gene	FlyBase ID	FBgn0010453
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	DWnt4, DWnt-4, Wnt-4
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	27E7-27E8	Sequence location	2L:7,255,419..7,277,168 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Wnt oncogene analog 4 is referred to in FlyBase by the symbol Dmel\Wnt4 (CG4698, FBgn0010453). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: female gonad development; establishment of imaginal disc-derived wing hair orientation; non-canonical Wnt receptor signaling pathway; retinal ganglion cell axon guidance; cell migration; neural retina development; regulation of hemocyte proliferation; salivary gland morphogenesis; Wnt receptor signaling pathway, planar cell polarity pathway. 24 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; organ system subdivision; abdominal segment; adult segment; external compound sense organ; peripheral nervous system; non-connected developing system; abdominal segment 10; embryonic foregut. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Wnt; Wnt protein, conserved site; Wnt-4 protein, insecta. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1632524_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Wnt4. Gene sequence location is 2L:7255419..7277168.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms frizzled binding
FB2013_02	Controlled Vocabulary Terms anterior/posterior pattern specification extracellular space establishment of ommatidial planar polarity negative regulation of transcription from RNA polymerase II promoter canonical Wnt receptor signaling pathway G-protein coupled receptor binding axis specification plasma membrane
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 27E7-27E8   Limits computationally determined from genome sequence between P{PZ}snRNP70K02107 and P{PZ}wg02657
Experimentally Determined Cytological Location
Cytogenetic map Notes References 27F-28A   (determined by in situ hybridisation)   (Gieseler et al., 1995)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes	Maps close to wg. (Graba et al., 1995)
Gene Model & Products
Please see the GBrowse view of Dmel\Wnt4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Wnt4-RA FBtr0089291  NM_057624   3364   539 Wnt4-RB FBtr0334799  NM_001273258   2935   539
Additional Transcript Data & Comments
Reported size (kB)	3.2 (northern blot) (Graba et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Wnt4-PA   FBpp0088345   58.7   539   9.29     NP_476972   AAF52499 Wnt4-PB   FBpp0306845   58.7   539   9.29     NP_001260187   AGB92723
Additional Polypeptide Data & Comments
Reported size (kDa)	389 (aa) (Graba et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Wnt protein, conserved site (IPR018161) Wnt (IPR005817) Wnt-4 protein, insecta (IPR026539)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF52499   AC009534   AF533773 AAN04479   AE014134 AGB92723   AI124296   AY095078 AAM11406   BI228808   BI356159   BP540871   BP541461   BP549202   BP549203   BP550667   BP552209   BP552210   BP553336   BP553337   BP555429   BP555430   BP558034   BP558035   BP558157   BP559421   BT120121   CL705927   CO177074   CO186390   CO275438   CO284694   CO286151   CO287610   CO294189   CO307297   CO315807   CO317960   CO344584   EC066987   EC080126   EC194829   EC203579   EC205138   EC207894   EC218753   EC224650   EC226787   EC241584   EC252606   GH748488   GH749362   GH750041   GH750115   GH751463   GH752307   GH752461   GH753449   GH755719   GH756851   GH757512   GH758091   GH759751   GH760796   GH772505   GH772635   GH772793   GH773050   GH774147   GH780020   GH781502   GH784447   GH785566   GH785933   GH786049   GH788761   GH788846   GH789422   GH789491   GH789688   GH791419   GH791485   GH794265   GH794755   GH796643   GH796905   GH797156   GH797888   GH798189   GH799904   GH801982   GH802317   GH802370   GH802679   GH803333   GH803682   GH805380   GH806303   GH810600   GH813429   GH816819   GH820175   GH825471   GH826641   GH828311   GH829862   GH830172   GH830352   GH830487   GH830741   GH832357   GH833937   GH838680   GH840218   GH842028   GH843987   GH844283   GH844533   GH844773   GH846299   GH846460   GH849650   GH851068   GH853795   GH857468   GH858905   GH860866   GH862370   GH862640   GH863822   GH864872   GH864888   GH866117   GH866442   GH867854   GH869750   GH869948   GH870088   GH871390   GH871664   GH872413   GH872797   GH872988   GH873402   GH873929   GH874420   GH876426   GH877691   GH878436   GH878591   GH878913   GH879819   GH880048   GH880802   GH881089   GH882178   GH883659   GH883750   GH884608   GH884987   GH888275   GH890265   GH892483   GH893391   GH895017   GH895284   GH895880   GH897394   GH898789   GH904257   GH904961   GH905540   GH906354   GH908407   GH909012   GH909523   GH909888   GH910366   GH912315   GH912455   GH914358   GH914657   GH916242   GH921429   GH921522   GH922167   GH922463   GH922574   GH922940   GH923779   GH924504   GH928499   GH929848   GH930181   GH931022   GH931436   GH931713   GH932255   GH932341   GH934098   GH936193   GH936369   GH936375   GH938179   GH938427
UniProtKB/Swiss-Prot	P40589
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Graba et al., 1995) presumptive embryonic/larval central nervous system   (Graba et al., 1995) ectoderm   (Graba et al., 1995) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage foregut anlage   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 8 -- 11   organism | segment polarity expression pattern   (Graba et al., 1995) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium foregut primordium   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11   organism | segmentally repeated   (Sato et al., 1999) parasegment 4 of visceral mesoderm   (Sato et al., 1999) ventral nerve cord   (Sato et al., 1999) head segment | segmentally repeated   (Buratovich et al., 2000) epidermis | segmentally repeated & ventral   (Buratovich et al., 2000) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) clypeo-labral primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) embryonic ganglion mother cell   (Fisher et al., 2012) embryonic inner optic lobe primordium   (Fisher et al., 2012) embryonic leading edge cell primordium   (Fisher et al., 2012) Comment:reported as embryonic leading edge cell specific anlage embryonic optic lobe primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) gnathal primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) longitudinal visceral muscle primordium   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic anal pad   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic esophagus   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic inner optic lobe   (Fisher et al., 2012) embryonic large intestine   (Fisher et al., 2012) embryonic optic lobe   (Fisher et al., 2012) embryonic proventriculus   (Fisher et al., 2012) embryonic rectum   (Fisher et al., 2012) embryonic small intestine   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) gastric caecum   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 14   embryonic/larval foregut   (Graba et al., 1995) parasegment 4 of visceral mesoderm   (Graba et al., 1995) parasegment 8 of visceral mesoderm   (Graba et al., 1995) embryonic/larval hindgut   (Graba et al., 1995) embryonic stage 16   gastric caecum   (Graba et al., 1995) midgut constriction 2   (Graba et al., 1995)
Additional Descriptive Data
	Wnt4 transcripts are first detected in stage 8 embryos where they appear at the anterior tip and in a segment polarity pattern along the trunk. 15 stripes are observed in the posteriormost cells of each parasegment. They are one cell wide and overlap the ventralmost wg cells. Shortly after, expression is seen in the foregut primordium and in dorsal patches that are thought to correspond to cells of the labral and ocular segment primordia. By stage 14, transcripts are visible in the CNS and the developing gut. CNS expression is not uniform but increases, progressively from anterior to posterior regions of the embryo. In the gut, transcripts are seen in the foregut, hindgut, and in two regions of the visceral mesoderm corresponding to parasegments 4 and 8. The latter two sites correspond to the primordia of the gastric caeca and the second midgut constriction. (Graba et al., 1995)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference pupal stage   epithelial sheath   (Cohen et al., 2002) adult stage   germarium region 2   (Cohen et al., 2002) terminal filament basal cell   (Cohen et al., 2002) germarium region 1   (Cohen et al., 2002) epithelial sheath   (Cohen et al., 2002)
Additional Descriptive Data
	A 2 h after pupal formation (APF) protein is detected in the apical cells as they migrate basally. At 16 h APF Wnt4-expressing cells have migrated between the terminal filaments and developing germaria. At 18 h APF these cells have moved between individual basal stalk clusters. Expression is detected in sheath, basal half of the terminal filament cells and regions I and II of the germarium in the adult. (Cohen et al., 2002)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cell surface inferred from direct assay (Sato et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    175 Larval Midgut    20 Larval Hindgut    20.2 Larval Malpighian Tubules    10.4 Larval Fat Body    19.5 Larval Salivary Gland    5 Larval Trachea    5.15 Larval Carcass    8.4 Adult Head    12.6 Adult Eye    5 Adult Brain    40.3 Adult Thoracic-Abdominal Ganglion    32.4 Adult Crop    9.8 Adult Midgut    14.9 Adult Hindgut    24.5 Adult Malpighian Tubules    9.4 Adult Fat Body    17.9 Adult Salivary Gland    22 Adult Heart    2.425 Adult VirginFemale Spermatheca    14.5 Adult InseminatedFemale Spermatheca    22.5 Adult Ovary    3 Adult Testis    20.2 Adult Male Accessory Gland    14.3 Adult Carcass    5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    5   NA  Brain    40.3   175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    32.4   NA  Crop    9.8   no informative data  Midgut    no informative data   20.2  Hindgut    24.5   no informative data  Malpighian Tubules    9.4   no informative data  Fat Body    no informative data   5  Salivary Gland    no informative data   NA  Heart    2.425   5.15  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    3   NA  Testis    20.2   NA  Male Accessory Gland    no informative data   8.4  Carcass    5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    51 central nervous system, pupae P8    59 head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8    33 carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3  (51) central nervous system, pupae P8  (59) head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8  (33) carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    51 central nervous system, pupae P8    59 head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8    33 carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    51 central nervous system, pupae P8    59 head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8    33 carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    51 central nervous system, pupae P8    59 head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8    33 carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3  (51) central nervous system, pupae P8  (59) head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8  (33) carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    51 central nervous system, pupae P8    59 head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8    33 carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    51 central nervous system, pupae P8    59 head, virgin 1-day female    2 head, virgin 4-day female    3 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    7 fat body, pupae P8    33 carcass, larvae L3 wandering    8 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr    27 embryo 08-10hr    29 embryo 10-12hr    38 embryo 12-14hr    34 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr  (27) embryo 08-10hr  (29) embryo 10-12hr  (38) embryo 12-14hr  (34) embryo 14-16hr  (30) embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr    27 embryo 08-10hr    29 embryo 10-12hr    38 embryo 12-14hr    34 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr    27 embryo 08-10hr    29 embryo 10-12hr    38 embryo 12-14hr    34 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr    27 embryo 08-10hr    29 embryo 10-12hr    38 embryo 12-14hr    34 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr  27 embryo 08-10hr  29 embryo 10-12hr  (38) embryo 12-14hr  (34) embryo 14-16hr  30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr    27 embryo 08-10hr    29 embryo 10-12hr    38 embryo 12-14hr    34 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    9 embryo 06-08hr    27 embryo 08-10hr    29 embryo 10-12hr    38 embryo 12-14hr    34 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    9 larva L1    7 larva L2    3 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    8 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    37 tumorous blood cells mbn2    0 ovary fGS/OSS    98 ovary OSC    133 ovary OSS    65 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2  (37) tumorous blood cells mbn2    0 ovary fGS/OSS  (98) ovary OSC  (133) ovary OSS  (65) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    37 tumorous blood cells mbn2    0 ovary fGS/OSS    98 ovary OSC  (133) ovary OSS    65 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    37 tumorous blood cells mbn2    0 ovary fGS/OSS    98 ovary OSC    133 ovary OSS    65 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    37 tumorous blood cells mbn2    0 ovary fGS/OSS    98 ovary OSC    133 ovary OSS    65 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2  (37) tumorous blood cells mbn2    0 ovary fGS/OSS  (98) ovary OSC  (133) ovary OSS  (65) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    37 tumorous blood cells mbn2    0 ovary fGS/OSS    98 ovary OSC  133 ovary OSS    65 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    37 tumorous blood cells mbn2    0 ovary fGS/OSS    98 ovary OSC    133 ovary OSS    65 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	BDGP expression data • RE26454 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010453 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4698-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Wnt4 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa lethal | partially Wnt4K2 lethal | pharate adult stage, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cSa partially lethal - majority die | larval stage | recessive Wnt4C1 Wnt4EMS23 Wnt4X1 viable, with Scer\GAL4pnr-MD237 Wnt4GD5347 Sterility Allele sterile | recessive Wnt4C1 Wnt4EMS23 Wnt4X1 Other Phenotypes Allele neuroanatomy defective Wnt4P23 neuroanatomy defective, with Scer\GAL4NP6099 Wnt4Scer\UAS.WT neuroanatomy defective (with Wnt4C1) Wnt4EMS23 neuroanatomy defective (with Wnt4EMS23) Wnt4C1 Wnt4P23 neuroanatomy defective (with Wnt4P23) Wnt4EMS23 planar polarity defective, with Scer\GAL4dpp.blk1 Wnt4Scer\UAS.cGa planar polarity defective, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa planar polarity defective (with Df(2L)spdj2) Wnt4K2 planar polarity defective | somatic clone, with Scer\GAL4Act5C.PP Wnt4Scer\UAS.cGa visible, with Scer\GAL4dpp.blk1 Wnt4Scer\UAS.cGa visible, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa visible | heat sensitive (with Df(2L)spdj2) Wnt4K2 Phenotype manifest in Allele abdominal segment Wnt4s.cGa abdominal segment 1 Wnt4a.cGa abdominal segment 2 Wnt4a.cGa abdominal segment 3 Wnt4a.cGa abdominal segment 4 Wnt4a.cGa abdominal segment 5 Wnt4a.cGa abdominal segment 6 Wnt4a.cGa abdominal segment 7 Wnt4a.cGa abdominal segment 8 Wnt4a.cGa abdominal segment 9 Wnt4a.cGa abdominal segment 10 Wnt4a.cGa abdominal segment 11 Wnt4a.cGa alula | ectopic | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa anterior crossvein, with Scer\GAL4dpp.blk1 Wnt4Scer\UAS.cGa anterior crossvein, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa denticle | ectopic, with Scer\GAL4arm.PS Wnt4Scer\UAS.cSa denticle | ectopic, with Scer\GAL4da.G32 Wnt4Scer\UAS.cGa denticle belt | ectopic, with Scer\GAL4da.G32 Wnt4Scer\UAS.cGa denticle row 1 Wnt4a.cGa denticle row 2 Wnt4a.cGa dorsal denticle, with Scer\GAL4da.G32 Wnt4Scer\UAS.cGa dorsocentral bristle | supernumerary, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa egg chamber Wnt4unspecified embryonic/first instar larval cuticle, with Scer\GAL4da.G32 Wnt4Scer\UAS.cGa embryonic/larval optic stalk (with Wnt4C1) Wnt4EMS23 embryonic/larval optic stalk (with Wnt4EMS23) Wnt4C1 embryonic epidermis | dorsal, with Scer\GAL4da.G32 Wnt4Scer\UAS.cGa embryonic ventral epidermis, with Scer\GAL4arm.PS Wnt4Scer\UAS.cSa eye | ventral compartment (with Df(2L)spdj2) Wnt4K2 eye equator | heat sensitive (with Df(2L)spdj2) Wnt4K2 eye photoreceptor cell Wnt4P23 eye photoreceptor cell, with Scer\GAL4NP6099 Wnt4Scer\UAS.WT eye photoreceptor cell (with Df(2L)spdj2) Wnt4K2 eye photoreceptor cell (with Wnt4C1) Wnt4EMS23 eye photoreceptor cell (with Wnt4EMS23) Wnt4C1 Wnt4P23 eye photoreceptor cell (with Wnt4P23) Wnt4EMS23 germarium Wnt4unspecified haltere | ectopic | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa lamina Wnt4P23 lamina, with Scer\GAL4NP6099 Wnt4Scer\UAS.WT lamina (with Wnt4C1) Wnt4EMS23 lamina (with Wnt4EMS23) Wnt4C1 Wnt4P23 lamina (with Wnt4P23) Wnt4EMS23 mesothoracic tergum | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa neuron Wnt4P23 neuron, with Scer\GAL4NP6099 Wnt4Scer\UAS.WT neuron (with Wnt4C1) Wnt4EMS23 neuron (with Wnt4EMS23) Wnt4C1 Wnt4P23 neuron (with Wnt4P23) Wnt4EMS23 ommatidium (with Df(2L)spdj2) Wnt4K2 ovarian sheath Wnt4unspecified ovariole Wnt4C1 ovary Wnt4unspecified posterior postalar bristle | supernumerary, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa presumptive embryonic salivary gland Wnt4EMS23 presumptive embryonic salivary gland, with Scer\GAL4bap.3 Wnt4Scer\UAS.cGa scutellar bristle | supernumerary, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa scutellum | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa sternopleural bristle | supernumerary, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa wing, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa Wnt4Scer\UAS.cSa wing (with Df(2L)spdj2) Wnt4K2 wing | ectopic | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa wing disc | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa wing hair, with Scer\GAL4dpp.blk1 Wnt4Scer\UAS.cGa wing hair, with Scer\GAL4en-e16E Wnt4Scer\UAS.cGa wing hair, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa wing hair | cell non-autonomous | somatic clone, with Scer\GAL4Act5C.PP Wnt4Scer\UAS.cGa wing margin bristle | ectopic | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa wing sensillum | ectopic | heat sensitive, with Scer\GAL4ptc-559.1 Wnt4Scer\UAS.cGa wing vein | ectopic (with Df(2L)spdj2) Wnt4K2
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Wnt4 Class Mutagen Stocks Known lesion Wnt4C1 X ray 2 Yes Wnt4EMS23 loss of function allele ethyl methanesulfonate 2 Yes Wnt4EY06939 P-element activity 1 -- Wnt4MI03717 1 -- Wnt4K2 loss of function allele 0 Yes Wnt4P23 P-element activity 0 Yes Wnt4unspecified 0 -- Wnt4X1 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Wnt4 Class Mutagen Stocks Known lesion Wnt4GD5347 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Wnt4JF03378 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Wnt4KK102348 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Wnt4a.cGa in vitro construct 0 Yes Wnt4dsRNA.cMa in vitro construct - RNAi 0 -- Wnt4hs.3x.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Wnt4NIG.4698R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Wnt4s.cGa in vitro construct 0 Yes Wnt4S403A.Scer\UAS.3x.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - coding region fusion 0 Yes Wnt4Scer\FRT.αTub84B in vitro construct - regulatory fusion 0 Yes Wnt4Scer\UAS.3x.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Wnt4Scer\UAS.cGa in vitro construct - regulatory fusion 0 Yes Wnt4Scer\UAS.cPa.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Wnt4Scer\UAS.cSa in vitro construct - regulatory fusion 0 Yes Wnt4Scer\UAS.WT in vitro construct - regulatory fusion 0 Yes Wnt4αTub84B.PW FLPase 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)ade3 (Cohen et al., 2002) Df(2L)BSC226 (Christensen and Cook, 2006.12.5) Df(2L)BSC291 (Christensen and Cook, 2007.5.8) Df(2L)BSC324 (Christensen et al., 2007.10.29) Df(2L)DE (Cohen et al., 2002) Df(2L)Exel6017 (Ryder, 2004.4.26) Df(2L)RF (Cohen et al., 2002)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct M{UAS-Wnt4.3xHA} NA M{UAS-Wnt4.S403A.3xHA} NA P{GD5347} NA P{KK102348} NA P{NIG.4698R} NA P{TRiP.JF03378} NA P{UAS-Wnt4.G} NA P{UAS-Wnt4.HA.P} NA P{UAS-Wnt4.S} NA P{UAS-Wnt4.WT} NA heat-shock construct M{hs-Wnt4.3xHA} NA characterization construct P{αTub84B(FRT.wGMR)Wnt4} NA P{αTub84B(-FRT)Wnt4} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Wnt4EY06939 NA insertion of enhancer trap P{lacW}l(2)SH0553SH0553 No P{lArB}Wnt4K2 No miscellaneous insertions Mi{MIC}Wnt4MI03717 NA
Gene Ontology: Function, Process & Cellular Component ( 25 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
frizzled binding inferred from physical interaction with fz, fz2, fz4 (Wu and Nusse, 2002) protein binding inferred from physical interaction with fj (Lim et al., 2005)
Biological Process
CV term Evidence References
cell migration inferred from mutant phenotype (assigned by UniProtKB) (Cohen et al., 2002) establishment of imaginal disc-derived wing hair orientation inferred from genetic interaction with fj (Lim et al., 2005) female gonad development inferred from mutant phenotype (assigned by UniProtKB) (Cohen et al., 2002) neural retina development inferred from mutant phenotype (Sato et al., 2006) non-canonical Wnt receptor signaling pathway inferred from mutant phenotype (Sato et al., 2006) regulation of hemocyte proliferation inferred from mutant phenotype (Sinenko et al., 2010) retinal ganglion cell axon guidance inferred from mutant phenotype (Sato et al., 2006) salivary gland morphogenesis inferred from mutant phenotype (Harris and Beckendorf, 2007) Wnt receptor signaling pathway, planar cell polarity pathway inferred from mutant phenotype (Lim et al., 2005)
Cellular Component
CV term Evidence References
cell surface inferred from direct assay (Sato et al., 2006)
Terms Based on Predictions or Assertions ( 13 terms )
Molecular Function
CV term Evidence References
G-protein coupled receptor binding inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) receptor binding inferred from sequence or structural similarity with wg (FlyBase, 1992-)
Biological Process
CV term Evidence References
anterior/posterior pattern specification inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) axis specification inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) canonical Wnt receptor signaling pathway inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) cellular component movement traceable author statement (Pandur et al., 2002) establishment of ommatidial planar polarity inferred from biological aspect of ancestor with PANTHER:PTN000246564 (assigned by RefGenome) (Gaudet et al., 2010) focal adhesion assembly traceable author statement (Pandur et al., 2002) negative regulation of transcription from RNA polymerase II promoter inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) Wnt receptor signaling pathway inferred from sequence or structural similarity with wg (Graba et al., 1995, FlyBase, 1992-)
Cellular Component
CV term Evidence References
extracellular region inferred from sequence model (Graba et al., 1995) inferred from sequence or structural similarity with wg (FlyBase, 1992-) extracellular space inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010) plasma membrane inferred from biological aspect of ancestor with PANTHER:PTN000246517 (assigned by RefGenome) (Gaudet et al., 2010)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Wnt4 allele Gene References Wnt4Scer\UAS.cGa hh (Buratovich et al., 2000) wg (Gieseler et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60153 DroID - A comprehensive database of gene and protein interactions. • FBgn0010453 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34007 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P40589
Orthologs
OrthoDB Orthologs (19) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62Z58Z Dipteran inclusive ortholog search EOG62Z4ZM Insect inclusive ortholog search EOG63BKJW Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG6XWTS2
Orthologs in Drosophila Species (EOG62Z58Z)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Wnt4     Drosophila simulans   Dsim\GD22501 Y   Drosophila sechellia   Dsec\GM13583 Y   Drosophila erecta   Dere\GG23540 Y   Drosophila yakuba   Dyak\GE18366 Y   Drosophila ananassae   Dana\GF14030 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA25881 Y   Drosophila persimilis   Dper\GL19231 Y   Drosophila willistoni   Dwil\GK24728 Y   Drosophila virilis   Dvir\GJ17616 Y   Drosophila mojavensis   Dmoj\GI17793 Y   Drosophila grimshawi   Dgri\GH13151 Y
Orthologs in non-Drosophila Dipterans (EOG62Z4ZM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000632   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010739   Anopheles gambiae Malaria mosquito  Agam\AGAP009735   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005836
Orthologs in non-Dipteran Insects (EOG63BKJW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Bombyx mori Silkmoth  Bmor\BGIBMGA006145   Tribolium castaneum Red flour beetle  Tcas\Wnt9
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6XWTS2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Xenopus tropicalis Western clawed frog  Xtro\wnt9b.2
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22501 Drosophila sechellia Dsec\GM13583 Drosophila erecta Dere\GG23540 Drosophila yakuba Dyak\GE18366 Drosophila ananassae Dana\GF14030 Drosophila pseudoobscura pseudoobscura Dpse\GA25881 Drosophila persimilis Dper\GL19231 Drosophila willistoni Dwil\GK24728 Drosophila virilis Dvir\GJ17616 Drosophila mojavensis Dmoj\GI17793 Drosophila grimshawi Dgri\GH13151
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	6651 w1118; Wnt4C1/CyO 29442 y1 v1; P{TRiP.JF03378}attP2 6650 Wnt4EMS23 bw1/CyO, P{HB-lacZ}GS1 36982 y1 w*; Mi{MIC}Wnt4MI03717/SM6a 20162 y1 w67c23; P{EPgy2}Wnt4EY06939/CyO 6907 y1 w1118; Wnt4C1 Wnt2L/CyO-Df(2R)B80, y+
Kyoto	108974 Wnt4EMS23 bw1/CyO, P{HB-lacZ}GS1
VDRC	v104671 P{KK102348}VIE-260B v38010 w1118; P{GD5347}v38010 v38011 w1118; P{GD5347}v38011/CyO
Genomic Clones ( 2 )
	BACR01E19 BACR10E07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 191 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE26454
Other clones	FI03360
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE42237
Other clones	78897 148524 159706 174780 273277 AI124296 CK01.20C.B6 CK01.22C.B2 CK01.64C.D1 CK01.79D.F3 CK01.97C.D5 EC252606 EK019239 EK052410 EK061922 EK066307 EK117606 EK166555 EK206263 EK254315 EK262515 EK269905 EP19540 FGA008F05 FGC001H07 FGM004C02 FGM208D11 FGM217D12 FGM223H06 FGM235C03 FGM312F04 FGM313C10 FGM320H03 RP003000434 RP003003175 RP003003949 RP003015498 RP003022101 RP003038334 RP003038835 RP003045813 RP003072584 RP003076967 RP003077265 RP003081688 RP003082733 RP003087397 RP003094759 RP003095584 RP003098077 RP003099259 RP003099925 RP003107815 RP003117935 RP003124441 RP003130119 RP003136343 RP003140643 RP003142419 RP003143670 RP003149099 RP003151942 RP003157170 RP003158712 RP003160140 RP003162908 RP003168909 RP003170386 RP003176031 RP003179827 RP003188283 RP003190317 RP003190459 RP003193711 RP003207832 RP003219573 RP003221565 RP003223541 RP003225530 RP003225994 RP003236310 RP003246964 RP003260673 RP003261566 RP003261998 RP003262415 RP003262937 RP003272227 RP003278915 RP003282697 RP003284502 RP003288757 RP003295070 RP003295704 RP003298760 RP003308600 RP003309776 RP003310896 RP003311551 RP003314369 RP003319841 RP003319944 RP003320387 RP003322634 RP003326897 RP003328097 RP003330387 RP003331417 RP003341202 RP003344135 RP003348641 RP003351291 RP003351770 RP003353360 RP003355354 RP003363198 RP003367879 RP003376696 RP003377413 RP003385197 RP003386896 RP003392874 RP003407004 RP003408241 RP003409911 RP003409981 RP003423248 RP003424672 RP003426266 RP003429282 RP003443197 RP003450156 RP003452581 RP003462144 RP003463798 RP003464860 RP003465236 RP003467616 RP003469459 RP003470405 RP003474173 RP003476107 RP003476854 RP003477806 RP003485515 RP003488286 RP003489593 RP003494415 RP003500061 RP003500326 RP003505669 RP003526158 RP003527691 RP003538048 RP003538907 RP003545244 RP003545286 RP003545497 RP003548539 RP003548890 RP003573335 RP003576233 RP003579757 RP003580145 RP003583210 RP003587343 RP003588655 RP003588776 RP003593938 RP003594813 RP003600369 RP003600543 RP003604306 RP003606149 RP003613854 RP003619435 RP003621127 RP003632101 RP003644227 RP003649064 RP003660456 RP003661660 RP003661784 RP003664739 RP003664958 RP003665106 RP003668668 RP003681524
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010453 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34007
Antibody Information
	polyclonal (Cohen et al., 2002)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Wnt4 CG4698 (Deng, 1999.11)
Source for database merge of
Additional comments
Other Comments
	Wnt4 is required for normal salivary gland migration in the embryo. It is required in the second phase of salivary gland migration, as the gland moves posteriorly within the embryo. (Harris and Beckendorf, 2007) Wnt4 regulates the dorsoventral specificity of retinal projections. (Sato et al., 2006) Wnt4 is required for cell movement and Fak56D regulation during ovarian morphogenesis. Wnt4 facilitates cell motility through regulation of focal adhesions. (Cohen et al., 2002) Wnt4 and wg produce distinct responses in cells of the dorsal embryonic epidermis. wg acts independently of hh signaling, while Wnt4 requires hh to elicit its effects. (Buratovich et al., 2000) Wnt4 has the capacity to antagonise wg signalling in the embryonic ventral epidermis. (Gieseler et al., 1999) wg and Wnt4 have partially overlapping embryonic expression patterns. Expression is regulated by HOM-C genes in the visceral mesoderm and segment polarity genes in the ectoderm. wg and Wnt4 achieve opposite, but complementary functions in intrasegmental cell patterning of the embryonic ectoderm. (Gieseler et al., 1995) Wnt4 has been characterised, Wnt4 gene product has a segment polarity-like expression pattern in the ectoderm and is controlled by homeotic complex (HOM-C) genes in the visceral mesoderm. (Graba et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057624 NM_001273258 RefSeq (Proteins) • NP_476972 NP_001260187 DDBJ / EMBL / GenBank • AAF52499 AAM11406 AAN04479 AC009534 AF533773 AGB92723 AI124296 AY095078 BI228808 CL705927 Entrez Gene - A searchable database of RefSeq genes. • 34007 UniProtKB/Swiss-Prot • P40589
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Wnt protein, conserved site (IPR018161) Wnt (IPR005817) Wnt-4 protein, insecta (IPR026539) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE26454
Linkouts
	BioGRID - A database of protein and genetic interactions • 60153 DroID - A comprehensive database of gene and protein interactions. • FBgn0010453 DRSC - Results from RNAi screens. • FBgn0010453 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010453 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4698-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010453 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34007 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/wnt4.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34007 modMine - Data generated by the modENCODE project. • FBgn0010453 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P40589
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	anon-EST:Liang-2.4   CG4698 (Keleman et al., 2009.8.5, Perkins et al., 2009) clone 2.4 (Liang and Biggin, 1998) Dm DWnt4 (Konikoff et al., 2010) DWnt4 (Coudreuse and Korswagen, 2007, Sato et al., 2005, Gupta and Schupbach, 2003, Wu and Nusse, 2002, Cadigan, 2002, Peifer and McEwen, 2002, Cohen et al., 2002, Cohen and Wilder, 2002, Llimargas and Lawrence, 2001, Gieseler et al., 2001, Cox et al., 2000, Rizkalla and Siegfried, 2000, Sato et al., 1999, Reifegerste and Moses, 1999, Mariol et al., 1997, Gieseler et al., 1995, Mariol et al., 1995, Gieseler et al., 1995, Sato et al., 2006, Lim et al., 2005, Port et al., 2008) Dwnt4 (Cadigan et al., 2002, Taylor, 2000, Terry et al., 2006, Joshi et al., 2007, Bovolenta et al., 2006) DWnt-4 (Pandur et al., 2002, Cohen and Wilder, 2001, Logan et al., 2001, Cohen et al., 2001, Wilder and Wallace, 2000, Wilder and Wallace, 2000, Cohen et al., 2000, Buratovich et al., 2000, Gieseler et al., 1999, Wilder et al., 1999, Buratovich and Wilder, 1999, Perrimon and Nusse, 1998, Buratovich and Wilder, 1998, Wilder et al., 1997, Cadigan and Nusse, 1997, Graba et al., 1997, Wilder et al., 1997, Graba et al., 1995, Chen et al., 2008) Wnt (Blair, 1999) Wnt4 (Pearson et al., 2005, Christensen and Cook, 2006.12.5, Christensen and Cook, 2007.5.8, Christensen et al., 2007.10.29, Herr and Basler, 2012, Casal et al., 2006, Ma et al., 2006, Harris and Beckendorf, 2007, Zeitouni et al., 2007, Chen et al., 2008, Inaki et al., 2007, Zeitlinger et al., 2007, Bina et al., 2010, Inaki et al., 2010, Cherbas et al., 2011, Lahaye et al., 2012, Peradziryi et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Kuroda et al., 2012) wnt4 (Veeman et al., 2003, Bhat, 1999, Bhat, 1998, Srahna et al., 2006, Sinenko et al., 2010) Wnt-4 (Bajpai et al., 2004, Jockusch and Ober, 2000, Wehrli and Tomlinson, 1998) wnt-4 (Liang and Biggin, 1998)
Name Synonym	Wnt oncogene analog 4
Secondary FlyBase IDs
References ( 108 )
Generate a list of	All references relating to this gene ( 108 )
List References by type	Research paper  ( 46 ) Supplementary material  ( 4 ) Review  ( 15 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 20 ) FlyBase analysis  ( 2 ) Conference report  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 2 ) Protein sequence record  ( 1 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 7 )
	Pitsouli and Perrimon, 2013, Sci. Signal. 6(263): ra12 The homeobox transcription factor cut coordinates patterning and growth during Drosophila airway remodeling. [FBrf0220848] Herr and Basler, 2012, Dev. Biol. 361(2): 392--402 Porcupine-mediated lipidation is required for Wnt recognition by Wls. [FBrf0217047] Kuroda et al., 2012, Mech. Dev. 128(11-12): 625--639 Canonical Wnt signaling in the visceral muscle is required for left-right asymmetric development of the Drosophila midgut. [FBrf0217619] Lahaye et al., 2012, PLoS ONE 7(3): e32297 WNT5 Interacts with the Ryk Receptors Doughnut and Derailed to Mediate Muscle Attachment Site Selection in Drosophila melanogaster. [FBrf0217725] Tauc et al., 2012, genesis 50(6): 466--481 A role for Drosophila Wnt-4 in heart development. [FBrf0218607] Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Peradziryi et al., 2011, EMBO J. 30(18): 3729--3740 PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling. [FBrf0215241] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...