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Gene Dmel\Sec61β

General Information
Symbol	Dmel\Sec61β	Species	D. melanogaster
Name	Sec61β	Annotation symbol	CG10130
Feature type	protein_coding_gene	FlyBase ID	FBgn0010638
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	51B6-51B6	Sequence location	2R:10,506,232..10,507,189 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Sec61β (CG10130, FBgn0010638). It has the cytological map location 51B6. Its sequence location is 2R:10506232..10507189. Its molecular function is described as protein transporter activity. It is involved in the biological process SRP-dependent cotranslational protein targeting to membrane, translocation. 11 alleles are reported. The phenotypes of these alleles are annotated with 13 unique terms, many of which group under: organ system; anatomical structure; egg; peripheral nervous system; adult segment; adult; cuticle; cell part; nervous system; thoracic segment. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 51B6-51B6   Limits computationally determined from genome sequence between P{lacW}ttvk03617&P{EP}ttvEP765 and P{lacW}Rpn6k00103
Experimentally Determined Cytological Location
Cytogenetic map Notes References 51B10-51B11   (Valcarcel, 1999.9.24) 51B10-51B11 (determined by in situ hybridisation)   (Valcarcel et al., 1999) 51B7-51B10 (determined by in situ hybridisation)   (Spradling et al., 1999) 51B7-51B10 (determined by in situ hybridisation)   (Perrimon et al., 1996) 51B7-51B10 51B7--8   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Sec61β for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Sec61beta-RA FBtr0087498  NM_143780   839   100
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Sec61beta-PA   FBpp0086627   10.2   100   12.17     NP_652037   AAF58229
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0010638
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Preprotein translocase Sec, Sec61-beta subunit (IPR005609) Preprotein translocase Sec, Sec61-beta subunit, eukarya (IPR016482)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390469   AC005646   AE003814 AAF58229   AI064020   AI257403   AI257815   AI517983   AI518193   AI519535   AI520501   AI542218   AI947091   AJ249754 CAB56698   AQ073507   AY071155 AAL48777   AY075554 AAL68361   BF490785   BG636089   BG640161   BG640504   BI171379   BI213250   BI238685   BI243133   BI361300   BI374790   BI486173   BI564465   BI578042   BI579283   BI612259   BI614574   BI615993   BI621417   BI631871   BI632388   BP549923   BP549924   BP552360   BP552361   BQ102820   CK662084   CO153106   CO184384   CO185598   CO271757   CO280318   CO282426   CO293479   CO302741   CO325398   CO330286   CO336739   EC061872   EC063023   EC073322   EC075278   EC086685   EC197591   EC202597   EC222239   EC222491   EC228909   EC235376   EC265113   EL872681   EL872682   EL872804   EL872805   EL874719   EL874720   G00586
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8SXW0 Q7JZN0
Maps to	DS00968 (Valcarcel et al., 1999)
Does NOT map to
Identified with	bs34h10 LD40681 (Oliver, 2001.8.16) LD09383 (Valcarcel et al., 1999) RE18615 (BDGP Project Members, 2000-) RH61539 (BDGP Project Members, 2000-, Abrams and Andrew, 2005) Dm0307 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12517
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010638 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0010638 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0010638 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Sec61β Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive Sec61β07214 Sec61βk03307 Sec61βk03316 Sec61βk07836 lethal | embryonic stage | germ-line clone | non-rescuable maternal effect | recessive Sec61β14448 lethal | embryonic stage | partially | rescuable maternal effect Sec61β07214 lethal | pupal stage | recessive Sec61β07214 Other Phenotypes Allele wild-type Sec61βTub.PV Phenotype manifest in Allele embryonic/first instar larval cuticle | germ-line clone Sec61β07214 embryonic/first instar larval cuticle Sec61β07214 Sec61β14448 Sec61βk07836 leg Sec61β07214 Sec61βk07836 head | germ-line clone Sec61β07214 abdomen | germ-line clone | posterior Sec61β07214 denticle belt Sec61β14448 epicuticle & embryo Sec61β14448 endocuticle & embryo Sec61β14448 embryonic/larval trachea Sec61β14448 dorsal appendage | germ-line clone Sec61β14448 eye | somatic clone Sec61β14448 ommatidium | somatic clone Sec61β14448 rhabdomere | somatic clone Sec61β14448 tarsal segment | somatic clone Sec61β14448 leg | somatic clone Sec61β14448 dorsal appendage Sec61βk03307 Sec61βk03316 Sec61βk16004 Sec61βk17010b egg Sec61βk16004 Sec61βk17010b chorion Sec61βk16004 Sec61βk17010b
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of Sec61β Class Mutagen Stocks Known lesion Sec61β07214 P-element activity 1 Yes Sec61β14448 P-element activity 0 Yes Sec61βEP2342 P-element activity 1 -- Sec61βk03307 P-element activity 3 -- Sec61βk03316 P-element activity 0 -- Sec61βk07819b P-element activity 0 Yes Sec61βk07836 P-element activity 0 -- Sec61βk16004 P-element activity 0 Yes Sec61βk17010b P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Sec61β Class Mutagen Stocks Known lesion Sec61βGD3787 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes Sec61βTub.PV in vitro construct | regulatory fusion 1 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)03072
Useful duplication	Dp(2;2)SMG45
Disrupted in	Df(2R)03072 (Spradling et al., 1999, Valcarcel et al., 1999, BDGP Project Members, 1994-1999) Df(2R)Exel6284 (Ryder, 2004.4.26) Df(2R)Exel7133 (Ryder, 2004.4.26)
Not disrupted in	Df(2R)trix (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{Tub-Sec61β.V} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Sec61β07214 Yes P{lacW}Sec61β14448 No P{lacW}Sec61βk03307 No P{lacW}Sec61βk03316 No P{lacW}Sec61βk07819b No P{lacW}Sec61βk07836 No P{lacW}Sec61βk16004 No P{lacW}Sec61βk17010b No P{lacW}l(2)SH1851SH1851 No insertion of mobile activating element P{EPgy2}EY03147 NA P{EP}Sec61βEP2342 NA
Related Comments
	Please look at the allele reports for the complete phenotype data The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. (Perrimon et al., 1996) Sec61β mutants die at the end of embryogenesis with a severe defect in the deposition of cuticle proteins. (Valcarcel et al., 1999)
Gene Ontology: Function, Process & Cellular Component ( 3 )
Molecular Function
CV term Evidence References
protein transporter activity inferred from sequence or structural similarity with UniProtKB:P38391 (FlyBase, 1992-)
Biological Process
CV term Evidence References
SRP-dependent cotranslational protein targeting to membrane, translocation inferred from sequence or structural similarity with UniProtKB:P38391 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
translocon complex non-traceable author statement (Valcarcel, 1999.9.24) inferred from sequence or structural similarity with UniProtKB:P38391 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Sec61β allele Gene References Sec61β07214 peb (Wilk et al., 2004) Sec61βk03307 Ras85D (Schnorr et al., 2001) peb (Wilk et al., 2004) Sec61βk03316 Ras85D (Schnorr et al., 2001) Sec61βk07819b Ras85D (Schnorr et al., 2001) Sec61βk16004 Ras85D (Schnorr et al., 2001) Sec61βk17010b Ras85D (Schnorr et al., 2001)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 70091
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11335 Dere\GG20468 Dgri\GH21806 Dmoj\GI20777 Dper\GL11450 Dpse\GA10096 Dsec\GM21558 Dsim\GD11064 Dvir\GJ20512 Dwil\GK19628 Dyak\Sec61β
Curated drosophilid orthologs	Dpse\GA10096   Dyak\Sec61β
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000014901 Anopheles gambiae (Ensembl) ENSANGP00000022309 Anopheles gambiae (Ensembl) ENSBTAP00000003190 Bos taurus (Ensembl) ENSCAFP00000003690 Canis familiaris (Ensembl) ENSGALP00000022049 Gallus gallus (Ensembl) ENSP00000223641 Homo sapiens (Ensembl) ENSMMUP00000013968 Macaca mulatta (Ensembl) ENSMODP00000015842 Monodelphis domestica (Ensembl) ENSPTRP00000036204 Pan troglodytes (Ensembl) ENSRNOP00000008329 Rattus norvegicus (Ensembl) GSTENP00034040001 Tetraodon nigroviridis (Ensembl) AT5G60460.1 Arabidopsis thaliana (Arabidopsis) WBGene00036649 Caenorhabditis briggsae (Wormbase) WBGene00021427 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040718-289 Danio rerio (Zfin) ZDB-GENE-040718-260 Danio rerio (Zfin) DDB0204435 Dictyostelium discoideum (Dictybase) MGI:1913462 Mus musculus (Informatics) SPBC2G2.03c Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Szeged	- P{EP}Sec61βEP2342 - P{lacW}l(2)SH1851SH1851
Bloomington	7335 P{Tub-Sec61β.V}1, w* 15626 y1 w67c23; P{EPgy2}EY03147/CyO 12339 P{PZ}Sec61β07214 cn1/CyO; ry506 10376 y1 w67c23; P{lacW}Sec61βk03307/CyO
VDRC	v8785 w1118; P{GD3787}v8785
Kyoto	102268 Df(1)w67c23, y1 ; P{lacW}Sec61βk03307 / CyO 102539 Df(1)w67c23, y1 ; P{lacW}Sec61βk03307 / CyO
Genomic Clones ( 2 )
	BACR27M04 BACR07P02
cDNA Clones ( 46 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RH61539 RE18615
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE73283 RH45932 SD11123 LP06292 LD09383 RE13137 RH52754 LD39159 RE71839 AT27371 RE40690 LD37354 SD13679 LD37638 RE35196 RE69560 LP22045 RH43975 RH40678 LP05757 SD26156 GH04267