FlyBase Gene Report: Dmel\MFS14

General Information

Symbol

Dmel\MFS14

Species

D. melanogaster

Name

Major Facilitator Superfamily Transporter 14

Annotation Symbol

CG15095

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010651

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Major Facilitator Superfamily Transporter 14 (MFS14) encodes a protein involved in response to hypoxia. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(2)08717, lethal (2) 08717

Key Links

Genomic Location

Cytogenetic map

55F3-55F4

Sequence location

2R:18,796,975..18,803,727 [-]

Recombination map

2-86

RefSeq locus

NT_033778 REGION:18796975..18803727

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables transmembrane transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000910504

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR011701, InterPro:IPR020846

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in response to hypoxia

inferred from expression pattern

(Azad et al., 2009)

inferred from mutant phenotype

(Azad et al., 2009)

involved_in wing disc dorsal/ventral pattern formation

inferred from genetic interaction with FLYBASE:Bx; FB:FBgn0265598,FLYBASE:ap; FB:FBgn0267978

(Bejarano et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in transmembrane transport

inferred from electronic annotation with InterPro:IPR011701

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Goshima et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000910504

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC17 FAMILY OF ORGANIC ANION TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Major Facilitator Superfamily Transporter 14 (MFS14) encodes a protein involved in response to hypoxia. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

SLC17 FAMILY OF ORGANIC ANION TRANSPORTERS -: Solute carrier family 17 (SLC17) members are transmembrane transporters of amino acids (glutamate and aspartate), nucleotides and organic anions. (Adapted from PMID:23506876, PMID:20059771 and FBrf0217562).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\MFS14 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8MRP7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MFS14-RA

FBtr0086635

NM_143781

2048

497

MFS14-RB

FBtr0086634

NM_166309

2999

497

MFS14-RC

FBtr0345426

NM_001299662

2337

497

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

MFS14-PA

FBpp0085817

54.8

497

8.59

FBpp0085816

54.8

497

8.59

FBpp0311551

54.8

497

8.59

Q8MRP7

NP_001286591

AHN56386

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

497 aa isoforms: MFS14-PA, MFS14-PB, MFS14-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MFS14 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.76

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 5 -- 7

organism | terminal expression pattern

(Laridon et al., 2008)

organism | pair rule expression pattern

(Laridon et al., 2008)

embryonic stage 6 -- 9

stomodeal invagination

(Laridon et al., 2008)

amnioproctodeal invagination

(Laridon et al., 2008)

embryonic stage 6 -- 13

yolk nucleus

(Laridon et al., 2008)

embryonic stage 7 -- 8

amnioserosa anlage

(Fisher et al., 2012)

anterior endoderm anlage

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 14 -- 15

embryonic/larval fat body

(Laridon et al., 2008)

embryonic stage 14 -- 16

embryonic/larval midgut

(Laridon et al., 2008)

embryonic stage 16

embryonic/larval salivary gland

(Laridon et al., 2008)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Goshima et al., 2007)

Expression Deduced from Reporters

Reporter: P{PTT-GA}MFS14^CA06962

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body glial cell

(DeSalvo et al., 2014)

adult brain subperineurial glial cell

(DeSalvo et al., 2014)

adult brain perineurial glial cell

(DeSalvo et al., 2014)

adult brain neuropil glial cell

(DeSalvo et al., 2014)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\MFS14 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MFS14

View exonic expression by tissue for Dmel\MFS14

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010651

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

46085/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010651

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010651

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010651

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15095

Flygut - An atlas of the Drosophila adult midgut

FBgn0010651

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\MFS14

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MFS14

Transgenic constructs containing regulatory region of MFS14

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(2R)Exel7158

(Ryder, 2004.4.26)

Df(2R)P34

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)PC29

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(2R)PC4

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)RM2-1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

MFS14^BG00842

lethal | recessive

MFS14⁰⁸⁷¹⁷

viable

MFS14^CA06962

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

MFS14^GD1653

viable, with Scer\GAL4^Mef2.PR

MFS14^GD1653

viable, with Scer\GAL4^pnr-MD237

MFS14^GD1653

viable, with Scer\GAL4^tin.CΔ4

MFS14^GD1653

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (13)

Hsap\SLC17A2

5 of 14

Yes

Hsap\SLC17A3

5 of 14

Yes

Hsap\SLC17A5

5 of 14

Yes

Hsap\SLC17A7

5 of 14

Yes

Hsap\SLC17A8

5 of 14

Yes

4 of 14

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Slc17a2

5 of 14

Yes

Rnor\Slc17a3

5 of 14

Yes

Rnor\Slc17a5

5 of 14

Yes

Rnor\Slc17a8

5 of 14

Yes

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Slc17a2

5 of 14

Yes

Mmus\Slc17a3

5 of 14

Yes

Mmus\Slc17a5

5 of 14

Yes

Mmus\Slc17a7

5 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\slc17a7

3 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tab3

1 of 13

Yes

Danio rerio (Zebrafish) (15)

Drer\slc17a5

5 of 14

Yes

Drer\slc17a7a

5 of 14

Yes

Drer\slc17a7b

5 of 14

Yes

Drer\slc17a8

5 of 14

Yes

Drer\si:ch1073-513e17.1

4 of 14

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (65)

Cele\cima-1

8 of 14

Yes

Cele\slc-17.5

7 of 14

Yes

Cele\slc-17.7

7 of 14

Yes

Cele\slc-17.8

7 of 14

Yes

Cele\slc-17.9

7 of 14

Yes

6 of 14

6 of 14

6 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\C39E9.7

2 of 14

Yes

Cele\D1046.4

2 of 14

Yes

Cele\F10C2.7

2 of 14

Yes

Cele\F41C3.2

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\H11E01.2

2 of 14

Cele\K08C7.1

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\T28H11.8

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\C06E7.88

1 of 14

Yes

Cele\C25H3.15

1 of 14

Yes

Cele\F47B8.10

1 of 14

Cele\F56A4.11

1 of 14

Yes

Cele\mfsd-10

1 of 14

Cele\T05H10.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\ZK54.3

1 of 14

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP004402

6 of 12

Yes

Agam\AgaP_AGAP007732

6 of 12

Yes

Agam\AgaP_AGAP008931

6 of 12

Yes

Agam\AgaP_AGAP009498

6 of 12

Yes

5 of 12

5 of 12

5 of 12

5 of 12

Yes

5 of 12

4 of 12

4 of 12

4 of 12

4 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (14)

Atha\ANTR2

6 of 13

Yes

Atha\PHT4;1

6 of 13

Yes

Atha\PHT4;2

6 of 13

Yes

Atha\PHT4;3

6 of 13

Yes

Atha\PHT4;5

6 of 13

Yes

Atha\PHT4;6

6 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\DAL5

4 of 13

Yes

Scer\THI73

4 of 13

Yes

Scer\YCT1

3 of 13

Yes

Scer\SOA1

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\SPCC417.10

4 of 12

Yes

Spom\SPCC757.13

4 of 12

Yes

Spom\liz1

3 of 12

Spom\SPAC11D3.18c

3 of 12

Yes

Spom\SPAC1002.16c

3 of 12

Yes

Spom\SPAC1039.04

3 of 12

Spom\SPBC1683.12

3 of 12

Spom\SPBC1773.15

3 of 12

Yes

Spom\SPBPB10D8.01

3 of 12

Yes

Spom\SPCPB1C11.03

3 of 12

Spom\SPAC1B3.15c

1 of 12

Yes

Spom\vht1

1 of 12

Yes

Escherichia coli (enterobacterium) (11)

Ecol\dgoT

8 of 11

Yes

Ecol\garP

8 of 11

Yes

Ecol\gudP

8 of 11

Yes

Ecol\lgoT

7 of 11

Yes

Ecol\exuT

5 of 11

Yes

Ecol\glpT

1 of 11

Ecol\mdtG

1 of 11

Ecol\tsgA

1 of 11

Yes

Ecol\uhpC

1 of 11

Ecol\uhpT

1 of 11

Ecol\yfaV

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:MFS14. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (28)

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

9 of 13

9 of 13

9 of 13

9 of 13

9 of 13

9 of 13

9 of 13

9 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

6 of 13

2 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

SLC17A5; solute carrier family 17 member 5

model of sialuria

IEA

(FlyBase, 2019-)

SLC17A8; solute carrier family 17 member 8

model of autosomal dominant nonsyndromic deafness 25

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC17A2; solute carrier family 17 member 2

5 of 14

611049

SLC17A3; solute carrier family 17 member 3

5 of 14

611034

URIC ACID CONCENTRATION, SERUM, QUANTITATIVE TRAIT LOCUS 4; UAQTL4

SLC17A5; solute carrier family 17 member 5

5 of 14

604322

sialuria

SLC17A7; solute carrier family 17 member 7

5 of 14

605208

SLC17A8; solute carrier family 17 member 8

5 of 14

607557

DEAFNESS, AUTOSOMAL DOMINANT 25; DFNA25

autosomal dominant nonsyndromic deafness 25

SLC17A1; solute carrier family 17 member 1

4 of 14

182308

SLC17A4; solute carrier family 17 member 4

4 of 14

604216

SLC17A6; solute carrier family 17 member 6

4 of 14

607563

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Dref

suppressible

MFS14

(Ida et al., 2009)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

70092

DroID - A comprehensive database of gene and protein interactions.

FBgn0010651

MIST (protein-protein) - An integrated Molecular Interaction Database

46085

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-86

Cytogenetic map

55F3-55F4

Sequence location

2R:18,796,975..18,803,727 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55F3-55F4

Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)03709⁰³⁷⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

55F2-55F3

(determined by in situ hybridisation)

(Spradling et al., 1999)

55F2-55F3

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-91.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

50810

y¹ w^*; P{PTT-GA}MFS14^CA06962

12356

P{PZ}MFS14⁰⁸⁷¹⁷ cn¹/CyO; ry⁵⁰⁶

13863

y¹ w^67c23; P{SUPor-P}MFS14^KG04987

12441

w¹¹¹⁸; P{GT1}MFS14^BG00842/CyO, P{sevRas1.V12}FK1

91899

y¹ sc^* v¹ sev²¹; P{TKO.GS05463}attP40

33999

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00962}attP2

603866

y¹ w^*; P{org-1.SM-RFP}Jon25Bi^18a P{tinC-GFP}cn^13b MFS14^S1842 P{HLH54F.LVM-RFP}16c/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

NIG-Fly

HMS00962

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00962}attP2

VDRC

v11117

w¹¹¹⁸; P{GD1653}v11117/TM3

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (31)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH07529

Other clones

DPiM_GH07529

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010651

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010651

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.

(Goshima et al., 2007)

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Showed no change of expression in Clk mutants in microarray experiments. Identified in S2/cycloheximide assay as a direct target of Clk mediated transcriptional regulation.

(McDonald and Rosbash, 2001)

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: l(2)08717 CG15095

(Misra, 2000.11.18)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: MFS14 l(2)08717

(Bergwitz et al., 2012)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

8717

(Harvie et al., 1998)

CG15095

(Tunstall et al., 2012, Mosqueira et al., 2010, Ni et al., 2010.12.1, Ida et al., 2009, Bejarano et al., 2008, Laridon et al., 2008, Goshima et al., 2007, Kadener et al., 2007, Gonzalez-Aguero et al., 2005, Wheeler et al., 2004, McDonald and Rosbash, 2001)

MFS14

(Reim, 2024.4.19, Ceder and Fredriksson, 2022, Levis, 2018.8.30, Mossman et al., 2017, Transgenic RNAi Project members, 2017-, Bergwitz et al., 2012)

l(2)08717

(DeSalvo et al., 2014, Neckameyer and Argue, 2013, Bergwitz et al., 2012, Azad et al., 2009, Ida et al., 2009, Leatherbarrow and Halfon, 2009, Buszczak et al., 2007, Choksi et al., 2006, Wheeler et al., 2004)

l208717

(Abruzzi et al., 2011)

lethal (2) 08717

(Laridon et al., 2008)

lethal(2)08717

(Laridon et al., 2008)

Name Synonyms

Major Facilitator Superfamily Transporter 14

lethal (2) 08717

Secondary FlyBase IDs

FBgn0034393

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

46085

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8MRP7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q8MRP7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8MRP7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010651

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

46085/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010651

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010651

FlyMine - An integrated database for Drosophila genomics

FBgn0010651

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15095

MARRVEL_MODEL - MARRVEL (model organism gene)

46085

Linkouts

BioGRID - A database of protein and genetic interactions.

70092

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010651

DroID - A comprehensive database of gene and protein interactions.

FBgn0010651

DRSC - Results frm RNAi screens

FBgn0010651

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010651

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010651

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15095

Flygut - An atlas of the Drosophila adult midgut

FBgn0010651

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

46085

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (62)

The following external sites may use different assemblies or annotations than FlyBase.

-0.76

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