FlyBase Gene Report: Dmel\Gug

General Information

Symbol

Dmel\Gug

Species

D. melanogaster

Name

Grunge

Annotation Symbol

CG6964

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010825

Gene Model Status

Current

Stock Availability

15 publicly available

Enzyme Name (EC)

Histone deacetylase (3.5.1.98)

Gene Snapshot

Grunge (Gug) encodes a nuclear repressor protein that likely responds to Egfr signaling to control cell behavior for normal developmental patterning. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

Atro, Atrophin, l(3)03928, l(3)01323

Key Links

Genomic Location

Cytogenetic map

66C13-66D1

Sequence location

3L:8,449,922..8,472,669 [+]

Recombination map

3-24

RefSeq locus

NT_037436 REGION:8449922..8472669

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

SANT-MYB DOMAIN TRANSCRIPTION REGULATORS

Pathway (FlyBase)

Negative Regulators of Epidermal Growth Factor Receptor Signaling Pathway

Negative Regulators of Hedgehog Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

DNA binding; histone deacetylase activity; repressing transcription factor binding; transcription corepressor activity

Predictions / Assertions

DNA binding; RNA polymerase II transcription factor binding; transcription corepressor activity

Catalytic Activity (EC)

Experimental Evidence

Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone (3.5.1.98)

Predictions / Assertions

Summaries

Gene Group (FlyBase)

SANT-MYB DOMAIN TRANSCRIPTION REGULATORS -: The SANT-MYB domain transcription regulators are essentially part of chromatin remodelling complexes and co-repressors. The SANT domain shows similarity with DNA-binding helix-turn-helix of Myb domain, and are involved in protein-protein interactions in the chromatin complexes. (Adapted from PMID:15040448).

Pathway (FlyBase)

Negative Regulators of Epidermal Growth Factor Receptor Signaling Pathway -: Negative regulators of Epidermal Growth Factor Receptor signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl) or acting on downstream effectors.
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.

Summary (Interactive Fly)

transcriptional cofactor for Even-skipped - positive regulator of - homolog of mammalian Atrophin

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Gug or the JBrowse view of Dmel\Gug for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gug-RA

FBtr0076616

NM_145106

8151

1966

Gug-RC

FBtr0076618

NM_176300

8217

1988

Gug-RD

FBtr0076619

NM_206303

8704

1985

Gug-RE

FBtr0305696

NM_001259741

8350

1985

Gug-RF

FBtr0305697

NM_001259742

8713

1988

Gug-RG

FBtr0305698

NM_001259743

8413

2006

Gug-RH

FBtr0305699

NM_079249

8704

1985

Gug-RI

FBtr0333048

NM_001274651

8698

1983

Gug-RJ

FBtr0333049

NM_001274652

8485

2007

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Gug-PA

FBpp0076343

208.2

1966

7.54

NP_659574

AAN12008

Gug-PC

FBpp0076345

210.8

1988

7.50

NP_788478

AAO41268

Gug-PD

FBpp0089427

210.5

1985

7.45

NP_996025

AAS65054

Gug-PE

FBpp0296976

210.5

1985

7.45

NP_001246670

AFH04341

Gug-PF

FBpp0296977

210.9

1988

7.56

NP_001246671

AFH04342

Gug-PG

FBpp0296978

213.0

2006

7.57

NP_001246672

AFH04343

Gug-PH

FBpp0296979

210.4

1985

7.50

NP_523973

AAF50413

Gug-PI

FBpp0305262

210.3

1983

7.61

NP_001261580

AGB94275

Gug-PJ

FBpp0305263

213.1

2007

7.48

NP_001261581

AGB94276

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1985 aa isoforms: Gug-PD, Gug-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gug using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gug_1
Gug_2

Linkouts

Gene Ontology (19 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

DNA binding

inferred from direct assay

(Wang et al., 2006)

histone deacetylase activity

inferred from direct assay

(Wang et al., 2006)

repressing transcription factor binding

inferred from physical interaction with FLYBASE:eve; FB:FBgn0000606

(Zhang et al., 2002)

inferred from physical interaction with FLYBASE:hkb; FB:FBgn0261434

(Zhang et al., 2002)

transcription corepressor activity

inferred from direct assay

(Zhang et al., 2002)

inferred from genetic interaction with FLYBASE:eve; FB:FBgn0000606

(Zhang et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001015306

(assigned by GO_Central )

(Gaudet et al., 2011)

RNA polymerase II transcription factor binding

inferred from biological aspect of ancestor with PANTHER:PTN001015306

(assigned by GO_Central )

(Gaudet et al., 2011)

transcription corepressor activity

inferred from biological aspect of ancestor with PANTHER:PTN001015306

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (12 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

embryonic pattern specification

inferred from mutant phenotype

(Zhang et al., 2002)

eye development

inferred from mutant phenotype

(Charroux et al., 2006)

imaginal disc-derived leg morphogenesis

inferred from mutant phenotype

(Erkner et al., 2002)

imaginal disc-derived wing vein morphogenesis

inferred from mutant phenotype

(Wang et al., 2008, Charroux et al., 2006)

inter-male aggressive behavior

inferred from mutant phenotype

(Davis et al., 2014)

larval somatic muscle development

inferred from expression pattern

(Dobi et al., 2014)

inferred from mutant phenotype

(Dobi et al., 2014)

negative regulation of epidermal growth factor receptor signaling pathway

inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256

(Charroux et al., 2006)

inferred from genetic interaction with FLYBASE:aos; FB:FBgn0004569

(Charroux et al., 2006)

inferred from genetic interaction with FLYBASE:rho; FB:FBgn0004635

(Charroux et al., 2006)

negative regulation of smoothened signaling pathway

inferred from mutant phenotype

(Zhang et al., 2013)

inferred from physical interaction with FLYBASE:ci; FB:FBgn0004859

(Zhang et al., 2013)

negative regulation of transcription, DNA-templated

inferred from direct assay

(Zhang et al., 2013, Zhang et al., 2002)

regulation of histone acetylation

inferred from mutant phenotype

(Zhang et al., 2013)

segment specification

inferred from high throughput mutant phenotype

(Luschnig et al., 2004)

segmentation

inferred from mutant phenotype

(Zhang et al., 2002)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

nucleus

inferred from direct assay

(Zhang et al., 2002)

polytene chromosome interband

inferred from direct assay

(Wang et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001015306

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

cell body rind region

(Davis et al., 2014)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

nucleus

inferred from direct assay

(Zhang et al., 2002)

polytene chromosome interband

inferred from direct assay

(Wang et al., 2008)

Expression Deduced from Reporters

Reporter: P{GAL4}Gug^AGiR

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

peripodial epithelium of wing disc

(Bloomington Drosophila Stock Center, 2017)

third instar larval stage

peripodial epithelium of wing disc

(Gibson et al., 2002)

wandering third instar larval stage

wing hinge primordium

(Pallavi and Shashidhara, 2003)

mesothoracic tergum primordium

(Pallavi and Shashidhara, 2003)

peripodial epithelium of wing disc

(Pallavi and Shashidhara, 2003)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Gug in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gug

View exonic expression by tissue for Dmel\Gug

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010825

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010825

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6964-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6964

Flygut - An atlas of the Drosophila adult midgut

FBgn0010825

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 32 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gug

Transgenic constructs containing regulatory region of Gug

Deletions and Duplications ( 5 )

Disrupted in

Df(3L)BSC13

(Deal and Cook, 2002.2.25)

Df(3L)BSC157

(Gresens and Cook, 2006.5.11)

Df(3L)BSC389

(Christensen et al., 2007.10.29)

Df(3L)BSC815

(Christensen et al., 2009.6.15)

Df(3L)h-i22

(BDGP Project Members, 1994-1999)

Not disrupted in

Df(3L)h-i22

(Spradling et al., 1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Eaat1.PR

lethal, with Scer\GAL4^elav.PU

Gug^75QN.UAS

lethal, with Scer\GAL4^elav-C155

Gug^HMS00756

lethal | embryonic stage | germline clone | recessive | rescuable maternal effect

Gug^3L-22-3

lethal | recessive

lethal - all die before end of embryonic stage

lethal - all die before end of embryonic stage | germline clone | maternal effect | recessive

Gug^3L-22-3

lethal - all die before end of embryonic stage | maternal effect

lethal - all die before end of larval stage | recessive

Gug^j5A3

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gug^GD4271

partially lethal, with Scer\GAL4^NP5247

Gug^EY14339

partially lethal | heat sensitive, with Scer\GAL4^NP5247

Gug^dsRNA.UAS

partially lethal - majority die

Gug^j5A3

viable

Gug^EY14339

viable, with Scer\GAL4^Mef2.PR

Gug^GD4271

viable, with Scer\GAL4^pnr-MD237

Gug^GD4271

viable, with Scer\GAL4^tin.CΔ4

Gug^GD4271

viable (with Gug¹¹)

Gug³⁵

viable (with Gug³⁵)

Gug¹¹

Sterility

Allele

fertile

Gug^EY14339

Other Phenotypes

Allele

behavior defective | male limited, with Scer\GAL4^elav-C155

cell polarity defective | cell non-autonomous | somatic clone

Gug^unspecified

cell polarity defective | somatic clone

Gug^unspecified

flightless, with Scer\GAL4^Mef2.PR

Gug^GD4271

increased cell death | third instar larval stage, with Scer\GAL4^NP5247

Gug^EY14339

locomotor behavior defective, with Scer\GAL4^NP5247

Gug^EY14339

neuroanatomy defective | progressive, with Scer\GAL4^GMR.PF

Gug^75QN.UAS

planar polarity defective (with Gug¹¹)

Gug³⁵

planar polarity defective (with Gug³⁵)

Gug¹¹

planar polarity defective | somatic clone

Gug³⁵

some die during embryonic stage

some die during embryonic stage | maternal effect

some die during embryonic stage | maternal effect | recessive

Gug^3L-22-3

some die during larval stage | recessive

Gug^j5A3

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gug^GD4271

visible, with Scer\GAL4^pnr-MD237

Gug^GD4271

visible | homeotic | recessive | somatic clone

Gug¹⁷⁵⁷

visible | recessive | somatic clone

visible | somatic clone

Phenotype manifest in

Allele

abdominal 2 ventral denticle belt | germline clone | maternal effect

Gug^3L-22-3

abdominal 4 ventral denticle belt | germline clone | maternal effect

Gug^3L-22-3

abdominal 5 ventral denticle belt | germline clone | maternal effect

Gug^3L-22-3

abdominal 6 ventral denticle belt | germline clone | maternal effect

Gug^3L-22-3

abdominal 8 ventral denticle belt | germline clone | maternal effect

Gug^3L-22-3

adult abdominal segment 5 | somatic clone

Gug¹⁷⁵⁷

adult abdominal segment 6 | somatic clone

Gug¹⁷⁵⁷

adult thorax | somatic clone

Gug³⁵

arista | somatic clone

arista lateral | somatic clone

Gug^Eal15

autophagosome, with Scer\GAL4^GMR.PU

autophagosome | supernumerary, with Scer\GAL4^ninaE.PT

Gug^75QN.UAS

bract | ectopic | somatic clone

Gug³⁵

cone cell | somatic clone | supernumerary

Gug³⁵

coxa | somatic clone

Gug³⁵

denticle belt | germline clone

Gug^unspecified

egg | germline clone | maternal effect

Gug¹⁷⁵⁷

embryo | germline clone | maternal effect

Gug¹⁷⁵⁷

embryonic/first instar larval cuticle | germline clone

Gug^unspecified

embryonic/first instar larval cuticle | rescuable maternal effect | ventral

Gug³⁵

embryonic/larval brain, with Scer\GAL4^NP5247

Gug^EY14339

embryonic/larval neuron | ectopic | germline clone

Gug^unspecified

embryonic/larval somatic muscle

Gug⁰³⁹²⁸

embryonic/larval somatic muscle (with Df(3L)BSC389)

Gug⁰³⁹²⁸

embryonic abdomen | germline clone | maternal effect

Gug^3L-22-3

embryonic epidermis | germline clone

Gug^unspecified

embryonic head

Gug³⁵

embryonic segment | germline clone | maternal effect

Gug^3L-22-3

embryonic segment | germline clone | non-rescuable maternal effect

embryonic segment | non-rescuable maternal effect

Gug³⁵

eye, with Scer\GAL4^GMR.PU

eye, with Scer\GAL4^ninaE.PT

eye | somatic clone

Gug^unspecified

eye photoreceptor cell, with Scer\GAL4^GMR.PU

Gug^UAS.cCa

eye photoreceptor cell, with Scer\GAL4^ninaE.PT

eye photoreceptor cell | drug conditional, with Scer\GAL4^GMR.PU

Gug^75QN.UAS

eye photoreceptor cell | progressive, with Scer\GAL4^GMR.PF

Gug^75QN.UAS

eye photoreceptor cell | somatic clone

femur | proximal | somatic clone

Gug³⁵

interommatidial bristle, with Scer\GAL4^GMR.PF

Gug^UAS.cCa

leg, with Scer\GAL4^NP5247

Gug^EY14339

leg | cell non-autonomous | somatic clone | ventral

Gug¹⁷⁵⁷

leg | somatic clone

Gug^Eal15

leg | somatic clone | ventral

Gug³⁵

leg sensillum & prothoracic femur | somatic clone

Gug³⁵

leg sensillum | cell non-autonomous | somatic clone

Gug³⁵

leg sensillum | somatic clone

Gug³⁵

mesothoracic tarsal bristle | somatic clone

Gug³⁵

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Gug^GD4271

mesothoracic tergum | somatic clone

Gug^unspecified

mesothoracic tibial apical bristle | somatic clone

Gug³⁵

metathoracic tarsal bristle | somatic clone

Gug³⁵

mitochondrion, with Scer\GAL4^GMR.PU

Gug^UAS.cCa

ocellar triangle | somatic clone

Gug³⁵

ommatidium, with Scer\GAL4^GMR.PF

Gug^UAS.cCa

ommatidium (with Gug¹¹)

Gug³⁵

ommatidium (with Gug³⁵)

Gug¹¹

ommatidium | cell non-autonomous | somatic clone

Gug³⁵

ommatidium | somatic clone

prothoracic tarsal bristle | somatic clone

Gug³⁵

prothoracic tibial apical bristle | somatic clone

Gug³⁵

prothoracic tibial transverse row bristle | somatic clone

Gug³⁵

retina | temperature conditional, with Scer\GAL4^GMR.PU

rhabdomere | somatic clone

Gug^unspecified

sex comb | somatic clone

Gug³⁵

tarsal segment, with Scer\GAL4^dpp.blk1

Gug^UAS.cCa

trichome of the posterior wing margin | somatic clone

Gug³⁵

triple row | somatic clone

Gug³⁵

trochanter | somatic clone

Gug³⁵

unguis | somatic clone

wing, with Scer\GAL4^C-580

Gug^UAS.cCa

wing, with Scer\GAL4^NP5247

Gug^EY14339

wing | somatic clone

Gug³⁵

wing hair | cell non-autonomous | somatic clone

Gug^unspecified

wing margin | somatic clone

Gug^unspecified

wing vein

Gug^Ubi-p63E.GFP

wing vein, with Scer\GAL4^C-580

Gug^UAS.cCa

wing vein | cell autonomous | somatic clone | supernumerary

Gug³⁵

wing vein | ectopic, with Scer\GAL4^dpp.blk1

wing vein | ectopic | somatic clone

wing vein | somatic clone

Gug³³³³

wing vein | somatic clone | supernumerary

Gug¹⁴⁹⁶⁷

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RERE

10 of 15

Yes

Hsap\ATN1

8 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rere

9 of 15

Yes

Mmus\Atn1

6 of 15

Yes

Rattus norvegicus (Norway rat) (3)

Rnor\Rere

6 of 13

Yes

Rnor\Atn1

4 of 13

Yes

Rnor\LOC100911672

3 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\atn1

3 of 12

Yes

Xtro\mier2

1 of 12

Xtro\rere

1 of 12

Yes

Danio rerio (Zebrafish) (3)

Drer\rerea

9 of 15

Yes

Drer\rereb

9 of 15

Yes

Drer\atn1

3 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\egl-27

7 of 15

Yes

Cele\F10E7.11

1 of 15

Cele\pqn-20

1 of 15

Yes

Cele\T07F8.4

1 of 15

Arabidopsis thaliana (thale-cress) (5)

Atha\AT1G09040

2 of 9

Yes

Atha\AT1G09050

2 of 9

Yes

Atha\AT1G55050

2 of 9

Yes

Atha\AT2G47820

1 of 9

Yes

Atha\GATA7

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900TW )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Gug

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19989

Drosophila persimilis

Dper\GL15500

Drosophila willistoni

Dwil\GK20351

Drosophila virilis

Dvir\GJ13040

Drosophila mojavensis

Dmoj\GI12899

Drosophila grimshawi

Dgri\GH15305

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502NT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010559

Glossina morsitans

Tsetse fly

Gmor\GMOY005375

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05437

Mayetiola destructor

Hessian fly

Mdes\Mdes002608

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W08MB )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis florea

Little honeybee

Aflo\3043009700

Apis mellifera

Western honey bee

Amel\GB54092

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11151

Bombus terrestris

Buff-tailed bumblebee

Bter\15208976

Linepithema humile

Argentine ant

Lhum\LH22841

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0031ad

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006681

Pediculus humanus

Human body louse

Phum\PHUM418040

Rhodnius prolixus

Kissing bug

Rpro\RPRC015233

Rhodnius prolixus

Kissing bug

Rpro\RPRC015203

Cimex lectularius

Bed bug

Clec\CLEC009756

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009485

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07500

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0EZS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011833

Strigamia maritima

European centipede

Smar\SMAR015539

Ixodes scapularis

Black-legged tick

Isca\ISCW023589

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59729.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g05340

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_59449

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0O1H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rere

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_002979gn

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000023596

Gallus gallus

Domestic chicken

Ggal\ATN1

Gallus gallus

Domestic chicken

Ggal\RERE

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

dentatorubral-pallidoluysian atrophy

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Gug^75QN.UAS

model of dentatorubral-pallidoluysian atrophy

CEA

(Nisoli et al., 2010)

Gug^66QC.UAS

model of dentatorubral-pallidoluysian atrophy

CEA

(Nisoli et al., 2010)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ATN1; atrophin 1

model of dentatorubral-pallidoluysian atrophy

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Gug^75QN.UAS

model of dentatorubral-pallidoluysian atrophy

is exacerbated by Atg1^GS10797

(Nisoli et al., 2010)

is exacerbated by Tor^TED.UAS

(Nisoli et al., 2010)

is ameliorated by wts^G4808

(Napoletano et al., 2011)

is exacerbated by ft⁸

(Napoletano et al., 2011)

is exacerbated by ft^G-rv

(Napoletano et al., 2011)

is exacerbated by sav³

(Napoletano et al., 2011)

is ameliorated by d^GC13

(Napoletano et al., 2011)

Gug^66QC.UAS

model of dentatorubral-pallidoluysian atrophy

is exacerbated by Tor^TED.UAS

(Nisoli et al., 2010)

is exacerbated by Atg1^GS10797

(Nisoli et al., 2010)

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RERE; arginine-glutamic acid dipeptide repeats

10 of 15

605226

NEURODEVELOPMENTAL DISORDER WITH OR WITHOUT ANOMALIES OF THE BRAIN, EYE, OR HEART; NEDBEH

ATN1; atrophin 1

8 of 15

607462

dentatorubral-pallidoluysian atrophy

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Gug - mir-8

quantitative reverse transcription pcr, luminiscence technology, necessary binding region

(Karres et al., 2007)

protein-protein

Physical Interaction

Assay

References

Gug - G9a

anti tag coimmunoprecipitation, anti tag western blot

(Wang et al., 2008)

Gug - HDAC1

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Zhang et al., 2013, Wang et al., 2008)

Gug - Trl

anti bait coimmunoprecipitation, western blot

(Yeung et al., 2017)

Gug - ci

anti tag coimmunoprecipitation, anti tag western blot, pull down, two hybrid

(Zhang et al., 2013)

Gug - ft

pull down, anti tag western blot, two hybrid

(Sing et al., 2014, Fanto et al., 2003)

Gug - sbb

anti bait coimmunoprecipitation, anti tag western blot, pull down, autoradiography

(Haecker et al., 2007)

Gug - svp

two hybrid, pull down, autoradiography

(Wang et al., 2006)

Gug - tll

bimolecular fluorescence complementation, fluorescence microscopy, pull down, autoradiography, two hybrid, anti bait coimmunoprecipitation, anti tag western blot

(Davis et al., 2014, Haecker et al., 2007, Wang et al., 2006)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Gug

enhanceable

aop

(Charroux et al., 2006)

Gug

enhanceable

Atg1

(Nisoli et al., 2010)

Gug

enhanceable

Atg5

(Nisoli et al., 2010)

Gug

suppressible

(Napoletano et al., 2011)

Gug

suppressible

DnaJ-1

(Nisoli et al., 2010)

Gug

enhanceable

eve

(Zhang et al., 2002)

Gug

enhanceable

(Napoletano et al., 2011)

Gug

enhanceable

hkb

(Zhang et al., 2002)

Gug

enhanceable

pnt

(Charroux et al., 2006)

Gug

enhanceable

sav

(Napoletano et al., 2011)

Gug

enhanceable

Tor

(Nisoli et al., 2010)

Gug

suppressible

wts

(Napoletano et al., 2011)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Gug

(Wehn and Campbell, 2006)

Antp

suppressible

Gug

(Kankel et al., 2004)

aop

enhanceable

Gug

(Charroux et al., 2006)

aos

enhanceable

Gug

(Charroux et al., 2006)

aos

suppressible

Gug

(Charroux et al., 2006)

btl::Egfr

suppressible

Gug|aop

(Charroux et al., 2006)

enhanceable

Gug

(Fanto et al., 2003)

ftz

enhanceable

Gug

(Kankel et al., 2004)

mir-8

suppressible

Gug

(Karres et al., 2007)

Pcl

suppressible

Gug

(Kankel et al., 2004)

rho

suppressible

Gug

(Charroux et al., 2006)

rho

enhanceable

Gug

(Charroux et al., 2006)

enhanceable

Gug

(Charroux et al., 2006)

suppressible

Gug

(Charroux et al., 2006)

Scr

suppressible

Gug

(Kankel et al., 2004)

trx

enhanceable

Gug

(Kankel et al., 2004)

Ubx

enhanceable

Gug

(Kankel et al., 2004)

enhanceable

Gug

(Erkner et al., 2002)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

70115

DroID - A comprehensive database of gene and protein interactions.

FBgn0010825

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

46156

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Epidermal Growth Factor Receptor Signaling Pathway -: Negative regulators of Epidermal Growth Factor Receptor signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl) or acting on downstream effectors.
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Hippo signaling pathway - fly

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-24

Cytogenetic map

66C13-66D1

Sequence location

3L:8,449,922..8,472,669 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66C13-66D1

Limits computationally determined from genome sequence between P{lacW}l(3)L0139^L0139 and P{PZ}Gug⁰³⁹²⁸&P{EP}CG6745^EP595b

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66C-66C

(Luschnig et al., 2004)

66D1-66D2

(Zhang, 2002.1.24, BDGP Project Members, 1994-1999)

66D-66D

(Erkner, 1999.12.20)

66D1-66D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

Experimentally Determined Recombination Data

Location

3-24

(FlyBase, 2016)

3-19.7

(Comeron et al., 2012)

3-26

(Erkner et al., 2002)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Bloomington

51414

y¹ v¹; P{TRiP.GLC01789}attP40

84972

y¹ w^*; PBac{Gug-GFP.FPTB}VK00031

6773

w^*; P{GAL4}Gug^AGiR/TM6B, Tb¹

20800

y¹ w^67c23; P{EPgy2}Gug^EY14339

13419

y¹ w^67c23; P{SUPor-P}Gug^KG01280 ry⁵⁰⁶

32961

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00756}attP2

Kyoto

113676

w^*; P{GawB}Gug^NP5315 / TM6, P{UAS-lacZ.UW23-1}UW23-1

104556

w^*; P{GawB}Gug^NP3609 / TM6, Sb¹ Tb¹

112948

w^*; P{GawB}Gug^NP2517 / TM3, Sb¹ Ser¹

112723

y^* w^*; P{GawB}Gug^NP1598 / TM6, P{UAS-lacZ.UW23-1}UW23-1

VDRC

v107413

P{KK100675}VIE-260B

v13687

w¹¹¹⁸; P{GD4271}v13687/TM3

Availability

Atrophin

(Wang et al., 2006)

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (44)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010825

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010825

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

46156

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Wang et al., 2008)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: l(3)03928 l(3)01323

(Beaton, 1999.12.12)

Source for merge of: Gug CG6964

(Bayraktaroglu, 2000.8.7)

Source for merge of: Gug Atro

(O'Farrell, 2002.4.27)

Additional comments

Other Comments

Gug is a direct target of mir-8.

(Karres et al., 2007)

Gug acts downstream or in parallel to rl.

(Charroux et al., 2006)

Gug is crucial for correct segmentation during embryogenesis.

(Erkner et al., 2002)

Gug is required in diverse developmental processes including early embryo patterning.

(Zhang et al., 2002)

Identification: Modifies tsh expression in imaginal disc.

(Erkner et al., 1997)

Gug is required for patterning of the ventral proximal leg; in mutant cells proximal patterns are replaced with more distal ones. Gug is also required in all ventrally located cells along the entire proximal-distal axis of the leg for specific ventral identities or processes.

(Erkner et al., 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 84 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

46156

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010825

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010825

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gug_1
Gug_2

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6964

Flygut - An atlas of the Drosophila adult midgut

FBgn0010825

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

351111

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

46156

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6964

modMine - A data warehouse for the modENCODE project

FBgn0010825

Linkouts

BioGRID - A database of protein and genetic interactions.

70115

DroID - A comprehensive database of gene and protein interactions.

FBgn0010825

DRSC - Results frm RNAi screens

FBgn0010825

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0010825

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6964-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010825

FlyMine - An integrated database for Drosophila genomics

FBgn0010825

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

46156

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Hippo signaling pathway - fly

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG6964

(Guven-Ozkan et al., 2016, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Raker et al., 2009, Karres et al., 2007, Pilot et al., 2006)

GUG

(Tsai and Wang, 2007)

atro

(Robin et al., 2019, Sharma and McNeill, 2013, Singh and Mlodzik, 2012, Bao et al., 2011, Hyun et al., 2009, Li et al., 2009, Baena-Lopez et al., 2008, Arbouzova and McNeill, 2007, Karres et al., 2007, Strutt and Strutt, 2005, Luschnig et al., 2004, Parkhurst and Delidakis, 2003, Strutt, 2003)

gug

(Robin et al., 2019, Paik et al., 2012, Link et al., 2007, Tyler et al., 2007, Cho and Irvine, 2004, Luschnig et al., 2004, Luschnig et al., 2004)

l(3)01323

(Nicholls and Gelbart, 1998)

l(3)03928

(Misra, 2000.8.25, Beaton, 1999.12.12, Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(3)S146907

(Link et al., 2007)

l(3)j5A3

l(3)rO116

Name Synonyms

Grunge

(Thomas et al., 2015, Xiong et al., 2015, Xu et al., 2015, Alkhori et al., 2014, Housden et al., 2014, Sadeqzadeh et al., 2014, Zhang et al., 2013, Williams et al., 2012, Raker et al., 2009, Martin et al., 2007, Wang and Tsai, 2007, Erkner et al., 1997)

atrophin

(Lucas et al., 2015, Mulakkal et al., 2014, Vallejo et al., 2011, Hyun et al., 2009, Li et al., 2009, Sopko et al., 2009, Karres et al., 2007, Tyler et al., 2007, Riley, 2006, Strutt and Strutt, 2005)

grunge

(Aldaz et al., 2010, Link et al., 2007, Link et al., 2007)

Secondary FlyBase IDs

FBgn0011373
FBgn0011523
FBgn0020427
FBgn0024868
FBgn0035897

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (131)

Report Sections

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