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Gene Dmel\Spn

General Information
Symbol	Dmel\Spn	Species	D. melanogaster
Name	Spinophilin	Annotation symbol	CG16757
Feature type	protein_coding_gene	FlyBase ID	FBgn0010905
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	l(3)06911
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	62E4-62E5	Sequence location	3L:2,505,245..2,554,292 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Spinophilin is referred to in FlyBase by the symbol Dmel\Spn (CG16757, FBgn0010905). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein phosphatase 1 binding. There is experimental evidence that it is involved in the biological process: inter-male aggressive behavior; olfactory behavior. 19 alleles are reported. No phenotypic data is available. It has 10 annotated transcripts and 10 annotated polypeptides. Protein features are: PDZ domain; Sterile alpha motif domain; Sterile alpha motif, type 1; Sterile alpha motif/pointed domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult brain, adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult midgut, larval Malpighian tubules, larval fat body, adult ovary. Gene sequence location is 3L:2505245..2554292.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 62E4-62E5   Limits computationally determined from genome sequence between P{PZ}slsrL182 and P{PZ}Spn06911
Experimentally Determined Cytological Location
Cytogenetic map Notes References 62E-62E     (Keegan et al., 2000) 62E-62E     (Keegan and Garza, 1998) 62E4-62E5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Spn for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated Alternative translation stop created by use of multiphasic reading frames within coding region.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Spn-RB FBtr0304613  NM_001259641   7334   2134 Spn-RC FBtr0304614  NM_001259642   10136   2134 Spn-RD FBtr0304615  NM_001259643   7343   2137 Spn-RE FBtr0304616  NM_001259644   6383   1817 Spn-RF FBtr0304617  NM_001259645   6520   1684 Spn-RG FBtr0304618  NM_001259646   7295   2121 Spn-RH FBtr0304619  NM_001259647   9544   2134 Spn-RI FBtr0304620  NM_001259648   8756   2116 Spn-RJ FBtr0304621  NM_001259649   7376   2148 Spn-RK FBtr0308203  NM_080516   7343   2137
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Spn-PB   FBpp0293155   232.0   2134   5.00     NP_001246570   AFH04241 Spn-PC   FBpp0293156   232.0   2134   5.00     NP_001246571   AFH04242 Spn-PD   FBpp0293157   232.4   2137   5.00     NP_001246572   AFH04243 Spn-PE   FBpp0293158   198.4   1817   4.89     NP_001246573   AFH04244 Spn-PF   FBpp0293159   183.3   1684   4.71     NP_001246574   AFH04245 Spn-PG   FBpp0293160   230.7   2121   4.99     NP_001246575   AFH04246 Spn-PH   FBpp0293161   232.0   2134   5.00     NP_001246576   AFH04247 Spn-PI   FBpp0293162   229.9   2116   4.98     NP_001246577   AFH04248 Spn-PJ   FBpp0293163   233.4   2148   5.02     NP_001246578   AFH04249 Spn-PK   FBpp0300523   232.4   2137   5.03     NP_536777   AAF47657
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sterile alpha motif, type 1 (IPR021129) Sterile alpha motif/pointed domain (IPR013761) Sterile alpha motif domain (IPR001660) PDZ domain (IPR001478)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264973   AA391424   AA392687   AA538662   AA697455   AA697456   AA697950   AA699243   AA941586   AA949680   AA951562   AE014296 AAF47657   AF285759 AAG41905   AE014296 AFH04241   AE014296 AFH04242   AE014296 AFH04243   AE014296 AFH04244   AE014296 AFH04245   AE014296 AFH04246   AE014296 AFH04247   AE014296 AFH04248   AE014296 AFH04249   AI110161   AI402185   AI513020   AI544119   AQ073335   AW943766   AY061010   AY118590 AAM49959   AY118851 AAM50711   BG637947   BG638163   BG638453   BG638565   BI351789   BI629235   BP540818   BP542924   BP546126   BT100323   BT120285   BT124916   BT133165   CO178394   CO180179   CO185421   CO265502   CO266980   CO273543   CO291155   CO296442   CO307589   CO310555   CO329912   CO338300   CO338899   CO344858   CZ466473   CZ470536   CZ470537   CZ470757   CZ475278   CZ484110   EC057476   EC061389   EC063480   EC077530   EC194814   EC205923   EC210475   EC218612   EC231069   EC233488   EC233999   EC236205   EC239380   EC245602   EC254982   EC255536   EC259699   EC259957   EC265305   EV579075   EV579136   EV581237   GO271081   GO271158
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MSF6 Q9W003 Q9GQA3 Q8MSU5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000436656 3L:2,553,322..2,553,690 evidence=Reporter construct (in vivo) (Aerts et al., 2010, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult brain, adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult midgut, larval Malpighian tubules, larval fat body, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    55 central nervous system, pupae P8    98 head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male    32 head, mated 4-day male    31 head, mated 20-day male    29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    44 ovary, mated 4-day female    48 testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3  (55) central nervous system, pupae P8  (98) head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male  (32) head, mated 4-day male  (31) head, mated 20-day male  (29) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  (32) digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (48) testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    55 central nervous system, pupae P8    98 head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male    32 head, mated 4-day male    31 head, mated 20-day male    29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    44 ovary, mated 4-day female    48 testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    55 central nervous system, pupae P8    98 head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male    32 head, mated 4-day male    31 head, mated 20-day male    29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    44 ovary, mated 4-day female    48 testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    55 central nervous system, pupae P8    98 head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male    32 head, mated 4-day male    31 head, mated 20-day male    29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    44 ovary, mated 4-day female    48 testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3  (55) central nervous system, pupae P8  (98) head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male  32 head, mated 4-day male  31 head, mated 20-day male  29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (48) testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    55 central nervous system, pupae P8    98 head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male    32 head, mated 4-day male    31 head, mated 20-day male    29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    44 ovary, mated 4-day female    48 testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    55 central nervous system, pupae P8    98 head, virgin 1-day female    18 head, virgin 4-day female    19 head, virgin 20-day female    13 head, mated 1-day female    18 head, mated 4-day female    16 head, mated 20-day female    12 head, mated 1-day male    32 head, mated 4-day male    31 head, mated 20-day male    29 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    32 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    11 digestive system, 4-day adult    15 digestive system, 20-day adult    15 fat body, larvae L3 wandering    22 fat body, white prepupae    8 fat body, pupae P8    25 carcass, larvae L3 wandering    19 carcass, 1-day adult    7 carcass, 4-day adult    6 carcass, 20-day adult    6 ovary, virgin 4-day female    44 ovary, mated 4-day female    48 testis, mated 4-day male    5 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    36 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr    28 embryo 16-18hr    27 embryo 18-20hr    23 embryo 20-22hr    33 embryo 22-24hr    27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (44) embryo 02-04hr  (36) embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr  (28) embryo 16-18hr  (27) embryo 18-20hr    23 embryo 20-22hr  (33) embryo 22-24hr  (27) larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day  (27) adult female 30day  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    36 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr    28 embryo 16-18hr    27 embryo 18-20hr    23 embryo 20-22hr    33 embryo 22-24hr    27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    36 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr    28 embryo 16-18hr    27 embryo 18-20hr    23 embryo 20-22hr    33 embryo 22-24hr    27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    36 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr    28 embryo 16-18hr    27 embryo 18-20hr    23 embryo 20-22hr    33 embryo 22-24hr    27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (44) embryo 02-04hr  (36) embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr  28 embryo 16-18hr  27 embryo 18-20hr    23 embryo 20-22hr  (33) embryo 22-24hr  27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day  27 adult female 30day  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    36 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr    28 embryo 16-18hr    27 embryo 18-20hr    23 embryo 20-22hr    33 embryo 22-24hr    27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    44 embryo 02-04hr    36 embryo 04-06hr    14 embryo 06-08hr    16 embryo 08-10hr    20 embryo 10-12hr    18 embryo 12-14hr    22 embryo 14-16hr    28 embryo 16-18hr    27 embryo 18-20hr    23 embryo 20-22hr    33 embryo 22-24hr    27 larva L1    10 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    14 white prepupae new    16 white prepupae 12hr    20 white prepupae 24hr    25 pupae 2d postWPP    23 pupae 3d postWPP    13 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    9 adult male 30day    8 adult female 01day    16 adult female 05day    27 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    4 embryonic 1182-4H    41 embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3    43 wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    57 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS    51 ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    4 embryonic 1182-4H  (41) embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3  (43) wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3  (57) eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS  (51) ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    4 embryonic 1182-4H    41 embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3    43 wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    57 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS    51 ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    4 embryonic 1182-4H    41 embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3    43 wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    57 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS    51 ovary OSC    20 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    4 embryonic 1182-4H    41 embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3    43 wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    57 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS    51 ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    4 embryonic 1182-4H  (41) embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3  (43) wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3  (57) eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS  (51) ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    4 embryonic 1182-4H    41 embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3    43 wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    57 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS    51 ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    4 embryonic 1182-4H    41 embryonic GM2    8 embryonic Kc167    3 embryonic S1    16 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    8 wing disc ML-DmD8    24 wing disc ML-DmD9    23 wing disc ML-DmD16-c3    43 wing disc ML-DmD21    9 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    57 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    9 tumorous blood cells mbn2    13 ovary fGS/OSS    51 ovary OSC    20 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (30) cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult  (37) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (38) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (30) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (28) Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult  (37) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (38) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    30 cold shock, 4-day adult    24 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    37 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    15 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010905 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16757-RA;GM16129;CG32295-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die Spn06911 partially lethal - majority die | recessive Spn06911 viable SpnBG01796 viable, with Scer\GAL4pnr-MD237 SpnGD9198 Other Phenotypes Allele behavior defective | recessive SpnBG01011 smell perception defective SpnBG01796 Phenotype manifest in Allele
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of Spn Class Mutagen Stocks Known lesion Spnc00455 piggyBac transposase 1 -- Spnc06245 piggyBac transposase 1 -- Spnc06515 piggyBac transposase 1 -- SpnCB-5495-3 1 -- Spnd06681 P-element activity 1 -- Spne03547 1 -- Spnf01597 1 -- SpnMB01935 1 -- SpnMI05102 1 -- SpnMI06873 1 -- SpnNP1611 1 -- Spn06911 P-element activity 0 Yes SpnBG01011 0 -- SpnBG01796 0 Yes SpnCPTI001457 piggyBac transposase 0 Yes Spnf01643 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Spn Class Mutagen Stocks Known lesion SpnGD9198 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SpnHM05167 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SpnKK100833 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)HR370 (BDGP Project Members, 1994-1999) Df(3L)M21 (BDGP Project Members, 1994-1999)
Not disrupted in	Df(3L)R-G7 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9198} NA P{KK100833} NA P{TRiP.HM05167} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Spn06911 No insertion of mobile activating element P{XP}Spnd06681 NA miscellaneous insertions Mi{MIC}SpnMI05102 NA Mi{MIC}SpnMI06873 NA P{GT1}SpnBG01011 NA P{GT1}SpnBG01796 NA P{RS3}SpnCB-5495-3 NA PBac{681.P.FSVS-1}SpnCPTI001457 NA PBac{PB}Spnc00455 NA PBac{PB}Spnc06245 NA PBac{PB}Spnc06515 NA PBac{RB}Spne03547 NA PBac{WH}Spnf01597 NA PBac{WH}Spnf01643 NA insertion of enhancer trap binary system Mi{ET1}SpnMB01935 No P{GawB}SpnNP1611 No
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
inter-male aggressive behavior inferred from mutant phenotype (Edwards et al., 2009) olfactory behavior inferred from mutant phenotype (Sambandan et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
protein phosphatase 1 binding inferred from sequence or structural similarity (Keegan et al., 2000)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Spn allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 70124 DroID - A comprehensive database of gene and protein interactions. • FBgn0010905 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 46194 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8MSU5
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6MSDHS Dipteran inclusive ortholog search EOG6Z8Z4W Insect inclusive ortholog search EOG6H9WFJ Arthropod inclusive ortholog search EOG64B8H7 Metazoa inclusive ortholog search EOG67HJGX
Orthologs in Drosophila Species (EOG6MSDHS)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Spn     Drosophila melanogaster fruit fly  Dmel\CG32829     Drosophila simulans   Dsim\GD13386 Y Y Drosophila sechellia   Dsec\GM14116 Y Y Drosophila sechellia   Dsec\GM13738   Y Drosophila sechellia   Dsec\GM19187   Y Drosophila erecta   Dere\GG14511 Y Y Drosophila erecta   Dere\GG20462   Y Drosophila erecta   Dere\GG16169   Y Drosophila erecta   Dere\GG12590   Y Drosophila yakuba   Dyak\GE21702 Y Y Drosophila pseudoobscura pseudoobscura   Dpse\GA30209   Y Drosophila pseudoobscura pseudoobscura   Dpse\GA14130 Y Y Drosophila persimilis   Dper\GL24607   Y Drosophila willistoni   Dwil\GK12507 Y Y Drosophila willistoni   Dwil\GK21196   Y Drosophila virilis   Dvir\GJ16049 Y Y Drosophila mojavensis   Dmoj\GI22992   Y Drosophila mojavensis   Dmoj\GI12533 Y Y
Orthologs in non-Drosophila Dipterans (EOG6Z8Z4W)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002251 Y Anopheles gambiae Malaria mosquito  Agam\AGAP005943 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ007358 Y
Orthologs in non-Dipteran Insects (EOG6H9WFJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB54230 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG018760 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG032298 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22817 Y Atta cephalotes Leafcutter ant  Acep\ACEP14207 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO16491 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23562 Y Linepithema humile Argentine ant  Lhum\LH19484 Y Linepithema humile Argentine ant  Lhum\LH19486 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17058 Y Solenopsis invicta Red fire ant  Sinv\SINV19651 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI003780 Y Bombyx mori Silkmoth  Bmor\BGIBMGA012129 Y Pediculus humanus Human body louse  Phum\PHUM305910 Y Tribolium castaneum Red flour beetle  Tcas\TC002905 Y
Orthologs in non-Insect Arthropods (EOG64B8H7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_118335 Y Daphnia pulex Water flea  Dpux\JGI_V11_336955 Y Daphnia pulex Water flea  Dpux\JGI_V11_62650 Y Ixodes scapularis Deer tick  Isca\ISCW009137 Y
Orthologs in non-Arthropod Metazoa (EOG67HJGX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\C43E11.6 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002170gn Y Danio rerio Zebrafish  Drer\si:ch211-269i23.2 Y Danio rerio Zebrafish  Drer\ENSDARG00000076431 Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13386 Drosophila sechellia Dsec\GM14116 Drosophila erecta Dere\GG14511 Drosophila yakuba Dyak\GE21702 Drosophila ananassae Dana\GF24861 Drosophila pseudoobscura pseudoobscura Dpse\GA14130 Drosophila willistoni Dwil\GK12507 Drosophila virilis Dvir\GJ16049 Drosophila mojavensis Dmoj\GI12533 Drosophila grimshawi Dgri\GH15755
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	28956 y1 v1; P{TRiP.HM05167}attP2 40209 y1 w*; Mi{MIC}SpnMI05102 23380 y1 w67c23; Mi{ET1}SpnMB01935
Harvard	- P{XP}Spnd06681 - PBac{PB}Spnc00455 - PBac{PB}Spnc06245 - PBac{PB}Spnc06515 - PBac{RB}Spne03547 - PBac{WH}Spnf01597
Kyoto	123661 w1118; P{RS3}SpnCB-5495-3
VDRC	v105888 P{KK100833}VIE-260B v19658 w1118; P{GD9198}v19658
	More stocks available...
Genomic Clones ( 1 )
	BACR27A03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 61 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM16129 HL02501 LD45234 LP20995
Other clones	FI18374 MIP17038 MIP20585
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL02513 HL02514 HL03519 HL08024 LD08645 LD11470 LD32145 LD46353 RH58072
Other clones	33027 34677 36901 44016 50256 69925 80475 148738 191329 214219.22 214283.30 214307.19 214345.24 571251 CK01.6B.D11 CK01.22A.F7 CK01.28A.B10 CK01.71B.D7 CK01.98D.F5 Dmel_dig_000387_0656_0278 Dmel_dig_008629_0667_0463 Dmel_dig_011261_0646_0889 DMG7-chr3L.5.004.pred.a EC01151 EK009316 EK056616 EK076323 EK111404 EK142822 EK148058 EK213646 EK222007 EK255031 EK294141 EN16431 EP00139 EP20037 FGA008B09 FGC017F11 FGH103B03 GH09652 LD09846 LD18243 LD25791 LD29656
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010905 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 46194
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: E62 CG16757 (FlyBase, 1996-) Source for merge of: E62 l(3)06911 (Keegan et al., 2001) Source for merge of: Spn BcDNA:GM16129 (FlyBase, 1996-)
Additional comments	Release 2 annotation CG16757 split into CG32295 and CG16757 (which corresponds to Spn) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of Spn BcDNA:GM16129 was a shared cDNA (date:030728). (FlyBase, 1996-) Source for merge of E62 CG16757 was sequence comparison (date:001223). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Transcription unit spans the central region of the early-late puff at 62E. (Keegan et al., 2000) Cloning and molecular analysis has identified the Spn transcription unit spanning at least 20 kb of genomic DNA from the central region of the 62E puff that is induced following the metamorphic pulse of ecdysone at the end of the third larval instar. (Keegan and Garza, 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080516 NM_001259641 NM_001259642 NM_001259643 NM_001259644 NM_001259645 NM_001259646 NM_001259647 NM_001259648 NM_001259649 RefSeq (Proteins) • NP_536777 NP_001246570 NP_001246571 NP_001246572 NP_001246573 NP_001246574 NP_001246575 NP_001246576 NP_001246577 NP_001246578 DDBJ / EMBL / GenBank • AAF47657 AAG41905 AAM49959 AAM50711 AF285759 AFH04241 AFH04242 AFH04243 AFH04244 AFH04245 AFH04246 AFH04247 AFH04248 AFH04249 AI110161 AI402185 AI513020 AQ073335 AW943766 AY118590 AY118851 BI351789 CZ466473 CZ470536 CZ470537 CZ470757 CZ475278 CZ484110 Entrez Gene - A searchable database of RefSeq genes. • 46194 UniProtKB/TrEMBL • Q8MSF6 Q8MSU5 Q9GQA3 Q9W003
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sterile alpha motif, type 1 (IPR021129) Sterile alpha motif/pointed domain (IPR013761) Sterile alpha motif domain (IPR001660) PDZ domain (IPR001478)
Linkouts
	BioGRID - A database of protein and genetic interactions • 70124 DroID - A comprehensive database of gene and protein interactions. • FBgn0010905 DRSC - Results from RNAi screens. • FBgn0010905 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010905 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16757-RA;GM16129;CG32295-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010905 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 46194 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 46194 modMine - Data generated by the modENCODE project. • FBgn0010905 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8MSU5
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	BcDNA:GM16129   c62E-5 (Keegan et al., 2001) CG16757 (Altenhein et al., 2006, Maisonhaute et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Aerts et al., 2010, Cammarato et al., 2011) E62   EL62 (Keegan et al., 2000) l(3)06911 (Berkeley Drosophila Genome Project, 1993.6.1, BDGP Project Members, 1994-1999, ) Spn (Morozova et al., 2009, Aerts et al., 2010, Wasbrough et al., 2010, Hazelett et al., 2009) spn (Maisonhaute et al., 2007)
Name Synonym	d-spinophilin (Keegan et al., 2001, Keegan, 2000.7.7) Spinophilin (Keegan et al., 2001, Sambandan et al., 2006, Edwards et al., 2009, Cammarato et al., 2011)
Secondary FlyBase IDs
	FBgn0024370 FBgn0035350 FBgn0045360 FBgn0045813 FBgn0052295 FBgn0063195
References ( 42 )
Generate a list of	All references relating to this gene ( 42 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 2 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...