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Gene Dmel\Spn

General Information
Symbol	Dmel\Spn	Species	D. melanogaster
Name	Spinophilin	Annotation symbol	CG16757
Feature type	protein_coding_gene	FlyBase ID	FBgn0010905
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	l(3)06911
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	62E4-62E5	Sequence location	3L:2,505,245..2,554,292 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Spinophilin is referred to in FlyBase by the symbol Dmel\Spn (CG16757, FBgn0010905). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein phosphatase 1 binding. There is experimental evidence that it is involved in the biological process: inter-male aggressive behavior; olfactory behavior. 17 alleles are reported. No phenotypic data is available. It has 10 annotated transcripts and 10 annotated polypeptides. Protein features are: PDZ/DHR/GLGF; Sterile alpha motif domain; Sterile alpha motif, type 1; Sterile alpha motif/pointed domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult brain, adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult midgut, larval Malpighian tubules, larval fat body, adult ovary. Gene sequence location is 3L:2505245..2554292.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 6 of adulthood day 5 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 22 of adulthood day 20 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 35 of adulthood day 23 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood spermatozoon day 34 of adulthood day 44 of adulthood day 14 of adulthood male day 46 of adulthood References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Sequence features BKN20929 HFA07986 HFA08490 HFA08491
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 62E4-62E5   Limits computationally determined from genome sequence between P{PZ}slsrL182 and P{PZ}Spn06911
Experimentally Determined Cytological Location
Cytogenetic map Notes References 62E-62E     (Keegan et al., 2000) 62E-62E     (Keegan and Garza, 1998) 62E4-62E5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Spn for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Alternative translation stop created by use of multiphasic reading frames within coding region.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Spn-RB FBtr0304613     7334   2134 Spn-RC FBtr0304614     10136   2134 Spn-RD FBtr0304615     7343   2137 Spn-RE FBtr0304616     6383   1817 Spn-RF FBtr0304617     6520   1684 Spn-RG FBtr0304618     7295   2121 Spn-RH FBtr0304619     9544   2134 Spn-RI FBtr0304620     8756   2116 Spn-RJ FBtr0304621     7376   2148 Spn-RK FBtr0308203     7343   2137
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Spn-PB   FBpp0293155   232.0   2134   5.00       Spn-PC   FBpp0293156   232.0   2134   5.00       Spn-PD   FBpp0293157   232.4   2137   5.00       Spn-PE   FBpp0293158   198.4   1817   4.89       Spn-PF   FBpp0293159   183.3   1684   4.71       Spn-PG   FBpp0293160   230.7   2121   4.99       Spn-PH   FBpp0293161   232.0   2134   5.00       Spn-PI   FBpp0293162   229.9   2116   4.98       Spn-PJ   FBpp0293163   233.4   2148   5.02       Spn-PK   FBpp0300523   232.4   2137   5.03
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PDZ/DHR/GLGF (IPR001478) Sterile alpha motif domain (IPR001660) Sterile alpha motif, type 1 (IPR021129) Sterile alpha motif/pointed domain (IPR013761)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264973   AA391424   AA392687   AA538662   AA697455   AA697456   AA697950   AA699243   AA941586   AA949680   AA951562   AF285759 AAG41905   AI110161   AI402185   AI513020   AI544119   AQ073335   AW943766   AY061010   AY118590 AAM49959   AY118851 AAM50711   BG637947   BG638163   BG638453   BG638565   BI351789   BI629235   BP540818   BP542924   BP546126   BT100323   BT120285   BT124916   CO178394   CO180179   CO185421   CO265502   CO266980   CO273543   CO291155   CO296442   CO307589   CO310555   CO329912   CO338300   CO338899   CO344858   CZ466473   CZ470536   CZ470537   CZ470757   CZ475278   CZ484110   EC057476   EC061389   EC063480   EC077530   EC194814   EC205923   EC210475   EC218612   EC231069   EC233488   EC233999   EC236205   EC239380   EC245602   EC254982   EC255536   EC259699   EC259957   EC265305   EV579075   EV579136   EV581237   GO271081   GO271158
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MSF6 Q8MSU5 Q9GQA3 Q9W003
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0010905    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0010905 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0010905 expression level Developmental Stage   Expression Level embryo 00-02hr    4784 embryo 02-04hr    3860 embryo 04-06hr    1410 embryo 06-08hr    1694 embryo 08-10hr    2078 embryo 10-12hr    1843 embryo 12-14hr    2059 embryo 14-16hr    2996 embryo 16-18hr    2633 embryo 18-20hr    2306 embryo 20-22hr    3411 embryo 22-24hr    2661 larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new    1464 white prepupae 12hr    1803 white prepupae 24hr    2227 pupae 2d postWPP    1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day    1752 adult female 05day    2791 adult female 30day    2974 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4784) embryo 02-04hr  (3860) embryo 04-06hr  (1410) embryo 06-08hr  (1694) embryo 08-10hr  (2078) embryo 10-12hr  (1843) embryo 12-14hr  (2059) embryo 14-16hr  (2996) embryo 16-18hr  (2633) embryo 18-20hr  (2306) embryo 20-22hr  (3411) embryo 22-24hr  (2661) larva L1  (964) larva L2  (693) larva L3 12hr old  (442) larva L3 puffstage 1-2  (372) larva L3 puffstage 3-6  (1106) larva L3 puffstage 7-9  (1257) white prepupae new  (1464) white prepupae 12hr  (1803) white prepupae 24hr  (2227) pupae 2d postWPP  (1985) pupae 3d postWPP  (1230) pupae 4d postWPP  (891) adult male 01day  (910) adult male 05day  (907) adult male 30day  (826) adult female 01day  (1752) adult female 05day  (2791) adult female 30day  (2974) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4784) embryo 02-04hr  (3860) embryo 04-06hr  1410 embryo 06-08hr  (1694) embryo 08-10hr  (2078) embryo 10-12hr  (1843) embryo 12-14hr  (2059) embryo 14-16hr  (2996) embryo 16-18hr  (2633) embryo 18-20hr  (2306) embryo 20-22hr  (3411) embryo 22-24hr  (2661) larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new  1464 white prepupae 12hr  (1803) white prepupae 24hr  (2227) pupae 2d postWPP  (1985) pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day  (1752) adult female 05day  (2791) adult female 30day  (2974) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4784 embryo 02-04hr    3860 embryo 04-06hr    1410 embryo 06-08hr    1694 embryo 08-10hr    2078 embryo 10-12hr    1843 embryo 12-14hr    2059 embryo 14-16hr    2996 embryo 16-18hr    2633 embryo 18-20hr    2306 embryo 20-22hr    3411 embryo 22-24hr    2661 larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new    1464 white prepupae 12hr    1803 white prepupae 24hr    2227 pupae 2d postWPP    1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day    1752 adult female 05day    2791 adult female 30day    2974 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4784 embryo 02-04hr    3860 embryo 04-06hr    1410 embryo 06-08hr    1694 embryo 08-10hr    2078 embryo 10-12hr    1843 embryo 12-14hr    2059 embryo 14-16hr    2996 embryo 16-18hr    2633 embryo 18-20hr    2306 embryo 20-22hr    3411 embryo 22-24hr    2661 larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new    1464 white prepupae 12hr    1803 white prepupae 24hr    2227 pupae 2d postWPP    1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day    1752 adult female 05day    2791 adult female 30day    2974 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0010905 expression level Developmental Stage   Expression Level embryo 00-02hr    4784 embryo 02-04hr    3860 embryo 04-06hr    1410 embryo 06-08hr    1694 embryo 08-10hr    2078 embryo 10-12hr    1843 embryo 12-14hr    2059 embryo 14-16hr    2996 embryo 16-18hr    2633 embryo 18-20hr    2306 embryo 20-22hr    3411 embryo 22-24hr    2661 larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new    1464 white prepupae 12hr    1803 white prepupae 24hr    2227 pupae 2d postWPP    1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day    1752 adult female 05day    2791 adult female 30day    2974 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4784) embryo 02-04hr  (3860) embryo 04-06hr  (1410) embryo 06-08hr  (1694) embryo 08-10hr  (2078) embryo 10-12hr  (1843) embryo 12-14hr  (2059) embryo 14-16hr  (2996) embryo 16-18hr  (2633) embryo 18-20hr  (2306) embryo 20-22hr  (3411) embryo 22-24hr  (2661) larva L1  (964) larva L2  (693) larva L3 12hr old  (442) larva L3 puffstage 1-2  (372) larva L3 puffstage 3-6  (1106) larva L3 puffstage 7-9  (1257) white prepupae new  (1464) white prepupae 12hr  (1803) white prepupae 24hr  (2227) pupae 2d postWPP  (1985) pupae 3d postWPP  (1230) pupae 4d postWPP  (891) adult male 01day  (910) adult male 05day  (907) adult male 30day  (826) adult female 01day  (1752) adult female 05day  (2791) adult female 30day  (2974) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4784) embryo 02-04hr  (3860) embryo 04-06hr  1410 embryo 06-08hr  1694 embryo 08-10hr  2078 embryo 10-12hr  1843 embryo 12-14hr  2059 embryo 14-16hr  (2996) embryo 16-18hr  (2633) embryo 18-20hr  2306 embryo 20-22hr  (3411) embryo 22-24hr  (2661) larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new  1464 white prepupae 12hr  1803 white prepupae 24hr  2227 pupae 2d postWPP  1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day  1752 adult female 05day  (2791) adult female 30day  (2974) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4784 embryo 02-04hr    3860 embryo 04-06hr    1410 embryo 06-08hr    1694 embryo 08-10hr    2078 embryo 10-12hr    1843 embryo 12-14hr    2059 embryo 14-16hr    2996 embryo 16-18hr    2633 embryo 18-20hr    2306 embryo 20-22hr    3411 embryo 22-24hr    2661 larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new    1464 white prepupae 12hr    1803 white prepupae 24hr    2227 pupae 2d postWPP    1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day    1752 adult female 05day    2791 adult female 30day    2974 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4784 embryo 02-04hr    3860 embryo 04-06hr    1410 embryo 06-08hr    1694 embryo 08-10hr    2078 embryo 10-12hr    1843 embryo 12-14hr    2059 embryo 14-16hr    2996 embryo 16-18hr    2633 embryo 18-20hr    2306 embryo 20-22hr    3411 embryo 22-24hr    2661 larva L1    964 larva L2    693 larva L3 12hr old    442 larva L3 puffstage 1-2    372 larva L3 puffstage 3-6    1106 larva L3 puffstage 7-9    1257 white prepupae new    1464 white prepupae 12hr    1803 white prepupae 24hr    2227 pupae 2d postWPP    1985 pupae 3d postWPP    1230 pupae 4d postWPP    891 adult male 01day    910 adult male 05day    907 adult male 30day    826 adult female 01day    1752 adult female 05day    2791 adult female 30day    2974 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0010905    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0010905 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult brain, adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult midgut, larval Malpighian tubules, larval fat body, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass     Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass     modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010905 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16757-RA;GM16129;CG32295-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal Spn06911 semi-lethal | recessive Spn06911 viable SpnBG01796 viable, with Scer\GAL4pnr-MD237 SpnGD9198 Other Phenotypes Allele behavior defective | recessive SpnBG01011 smell perception defective SpnBG01796 Phenotype manifest in Allele
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of Spn Class Mutagen Stocks Known lesion Spnc00455 piggyBac transposase 1 -- Spnc06245 piggyBac transposase 1 -- Spnc06515 piggyBac transposase 1 -- SpnCB-5495-3 1 -- Spnd06681 P-element activity 1 -- Spne03547 1 -- Spnf01597 1 -- SpnMB01935 1 -- SpnNP1611 1 -- Spn06911 P-element activity 0 Yes SpnBG01011 0 -- SpnBG01796 0 Yes SpnCPTI001457 piggyBac transposase 0 Yes Spnf01643 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Spn Class Mutagen Stocks Known lesion SpnGD9198 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SpnHM05167 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SpnKK100833 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)HR370 (BDGP Project Members, 1994-1999) Df(3L)M21 (BDGP Project Members, 1994-1999)
Not disrupted in	Df(3L)R-G7 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9198} NA P{KK100833} NA P{TRiP.HM05167} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Spn06911 No insertion of mobile activating element P{XP}Spnd06681 NA miscellaneous insertions P{GT1}SpnBG01011 NA P{GT1}SpnBG01796 NA P{RS3}SpnCB-5495-3 NA PBac{681.P.FSVS-1}SpnCPTI001457 NA PBac{PB}Spnc00455 NA PBac{PB}Spnc06245 NA PBac{PB}Spnc06515 NA PBac{RB}Spne03547 NA PBac{WH}Spnf01597 NA PBac{WH}Spnf01643 NA insertion of enhancer trap binary system Mi{ET1}SpnMB01935 No P{GawB}SpnNP1611 No
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
inter-male aggressive behavior inferred from mutant phenotype (Edwards et al., 2009) olfactory behavior inferred from mutant phenotype (Sambandan et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
protein phosphatase 1 binding inferred from sequence or structural similarity (Keegan et al., 2000)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Spn allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 70124 DroID - A comprehensive database of gene and protein interactions. • FBgn0010905 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 46194
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24861 Dere\GG14511 Dgri\GH15755 Dmoj\GI12533 Dpse\GA14130 Dsec\GM14116 Dsim\GD13386 Dvir\GJ16049 Dwil\GK12507 Dyak\GE21702
Curated drosophilid orthologs	Dpse\GA14130
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL002251-PA Aedes aegypti (mosquito) AGAP005943-PA Anopheles gambiae (mosquito) XP_623895.2 Apis mellifera (honey bee) XP_001943518.1 Acyrthosiphon pisum (pea aphid) BGIBMGA012129-PA Bombyx mori (silk worm) CN10124 Caenorhabditis brenneri (small nematode) ENSCAFP00000025042 Canis familiaris (dog) CPIJ007358 Culex pipens (mosquito) ENSCPOP00000010404 Cavia porcellus (guinea pig) ENSECAP00000013846 Equus caballus (horse) ENSGACP00000007732 Gymnopilus aculeatus (mushroom) ENSP00000320510 Homo sapiens (human) ENSMODP00000032817 Monodelphis domestica (gray opossum) ENSMMUP00000034894 Macaca mulatta (rhesus monkey) ENSMUSP00000041732 Mus musculus (house mouse) ENSORLP00000021802 Oryzias latipes (japanese killifish) PHUM008432-PA Pediculus humanus (human body louse) ENSPPYP00000019958 Pongo pygmaeus (orangutan) ENSPTRP00000042311 Pan troglodytes (chimpanzee) ENSRNOP00000011756 Rattus norvegicus (norway rat) XP_975359.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020063 Tetraodon nigroviridis (green spotted pufferfish) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17058 NV23816 NV20951 NV17644 LH19486 LH19484 ISCW009137 FBgn0238950 FBgn0214517 FBgn0203234 FBgn0185100 FBgn0169045 JGI_V11_62650 JGI_V11_118335 JGI_V11_336955 JGI_V11_120489 FBgn0074159 FBgn0162196 FBgn0135290 FBgn0106770 CPIJ007358 BGIBMGA012129 ACYPI003780 GB13363 AGAP005943 AAEL002251 PHUM305910 GLEAN_02905
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	28956 y1 v1; P{TRiP.HM05167}attP2 23380 y1 w67c23; Mi{ET1}SpnMB01935
Harvard	- P{XP}Spnd06681 - PBac{PB}Spnc00455 - PBac{PB}Spnc06245 - PBac{PB}Spnc06515 - PBac{RB}Spne03547 - PBac{WH}Spnf01597
Kyoto	123661 w1118; P{RS3}SpnCB-5495-3 104048 y* w*; P{GawB}SpnNP1611 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v105888 P{KK100833}VIE-260B v19658 w1118; P{GD9198}v19658
Genomic Clones ( 1 )
	BACR27A03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 60 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM16129 HL02501 LD45234 LP20995
Other clones	MIP17038 MIP20585
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL02513 HL02514 HL03519 HL08024 LD08645 LD11470 LD32145 LD46353 RH58072
Other clones	33027 34677 36901 44016 50256 69925 80475 148738 191329 214219.22 214283.30 214307.19 214345.24 571251 CK01.6B.D11 CK01.22A.F7 CK01.28A.B10 CK01.71B.D7 CK01.98D.F5 Dmel_dig_000387_0656_0278 Dmel_dig_008629_0667_0463 Dmel_dig_011261_0646_0889 DMG7-chr3L.5.004.pred.a EC01151 EK009316 EK056616 EK076323 EK111404 EK142822 EK148058 EK213646 EK222007 EK255031 EK294141 EN16431 EP00139 EP20037 FGA008B09 FGC017F11 FGH103B03 GH09652 LD09846 LD18243 LD25791 LD29656
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010905 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 46194
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: E62 CG16757 (FlyBase, 1996-) Source for merge of: E62 l(3)06911 (Keegan et al., 2001) Source for merge of: Spn BcDNA:GM16129 (FlyBase, 1996-)
Additional comments	Release 2 annotation CG16757 split into CG32295 and CG16757 (which corresponds to Spn) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of Spn BcDNA:GM16129 was a shared cDNA (date:030728). (FlyBase, 1996-) Source for merge of E62 CG16757 was sequence comparison (date:001223). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Transcription unit spans the central region of the early-late puff at 62E. (Keegan et al., 2000) Cloning and molecular analysis has identified the Spn transcription unit spanning at least 20 kb of genomic DNA from the central region of the 62E puff that is induced following the metamorphic pulse of ecdysone at the end of the third larval instar. (Keegan and Garza, 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	DDBJ / EMBL / GenBank • AAF47657 AAG41905 AAM49959 AAM50711 AF285759 AI110161 AI402185 AI513020 AQ073335 AW943766 AY118590 AY118851 BI351789 CZ466473 CZ470536 CZ470537 CZ470757 CZ475278 CZ484110 Entrez Gene - A searchable database of RefSeq genes. • 46194 UniProtKB/TrEMBL • Q8MSF6 Q8MSU5 Q9GQA3 Q9W003
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • PDZ/DHR/GLGF (IPR001478) Sterile alpha motif domain (IPR001660) Sterile alpha motif, type 1 (IPR021129) Sterile alpha motif/pointed domain (IPR013761)
Linkouts
	BioGRID - A database of protein and genetic interactions • 70124 DroID - A comprehensive database of gene and protein interactions. • FBgn0010905 DRSC - Results from RNAi screens. • FBgn0010905 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010905 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16757-RA;GM16129;CG32295-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010905 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 46194 InParanoid A subset of ortholog calls from InParanoid. • AAEL002251-PA Aedes aegypti (mosquito) AGAP005943-PA Anopheles gambiae (mosquito) XP_623895.2 Apis mellifera (honey bee) XP_001943518.1 Acyrthosiphon pisum (pea aphid) BGIBMGA012129-PA Bombyx mori (silk worm) CN10124 Caenorhabditis brenneri (small nematode) ENSCAFP00000025042 Canis familiaris (dog) CPIJ007358 Culex pipens (mosquito) ENSCPOP00000010404 Cavia porcellus (guinea pig) ENSECAP00000013846 Equus caballus (horse) ENSGACP00000007732 Gymnopilus aculeatus (mushroom) ENSP00000320510 Homo sapiens (human) ENSMODP00000032817 Monodelphis domestica (gray opossum) ENSMMUP00000034894 Macaca mulatta (rhesus monkey) ENSMUSP00000041732 Mus musculus (house mouse) ENSORLP00000021802 Oryzias latipes (japanese killifish) PHUM008432-PA Pediculus humanus (human body louse) ENSPPYP00000019958 Pongo pygmaeus (orangutan) ENSPTRP00000042311 Pan troglodytes (chimpanzee) ENSRNOP00000011756 Rattus norvegicus (norway rat) XP_975359.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020063 Tetraodon nigroviridis (green spotted pufferfish) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 46194 modMine - Data generated by the modENCODE project. • FBgn0010905 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17058 NV23816 NV20951 NV17644 LH19486 LH19484 ISCW009137 FBgn0238950 FBgn0214517 FBgn0203234 FBgn0185100 FBgn0169045 JGI_V11_62650 JGI_V11_118335 JGI_V11_336955 JGI_V11_120489 FBgn0074159 FBgn0162196 FBgn0135290 FBgn0106770 CPIJ007358 BGIBMGA012129 ACYPI003780 GB13363 AGAP005943 AAEL002251 PHUM305910 GLEAN_02905
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	BcDNA:GM16129   c62E-5 (Keegan et al., 2001) CG16757 (Altenhein et al., 2006, Maisonhaute et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Aerts et al., 2010, Cammarato et al., 2011) E62   EL62 (Keegan et al., 2000) l(3)06911 (Berkeley Drosophila Genome Project, 1993.6.1, BDGP Project Members, 1994-1999, ) Spn (Morozova et al., 2009, Aerts et al., 2010, Wasbrough et al., 2010, Hazelett et al., 2009) spn (Maisonhaute et al., 2007)
Name Synonym	d-spinophilin (Keegan et al., 2001, Keegan, 2000.7.7) Spinophilin (Keegan et al., 2001, Sambandan et al., 2006, Edwards et al., 2009, Cammarato et al., 2011)
Secondary FlyBase IDs
	FBgn0024370 FBgn0035350 FBgn0045360 FBgn0045813 FBgn0052295 FBgn0063195
References ( 36 )
Generate a list of	All references relating to this gene ( 36 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 2 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Aerts et al., 2010, PLoS Biol. 8(7): e1000435 Robust Target Gene Discovery through Transcriptome Perturbations and Genome-Wide Enhancer Predictions in Drosophila Uncovers a Regulatory Basis for Sensory Specification. [FBrf0211419] Show all research papers ...