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Gene Dmel\msn

General Information
Symbol	Dmel\msn	Species	D. melanogaster
Name	misshapen	Annotation symbol	CG16973
Feature type	protein_coding_gene	FlyBase ID	FBgn0010909
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	l(3)06946
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	62E6-62E7	Sequence location	3L:2,555,775..2,586,540 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene misshapen is referred to in FlyBase by the symbol Dmel\msn (CG16973, FBgn0010909). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein serine/threonine kinase activity; MAP kinase kinase kinase kinase activity. There is experimental evidence that it is involved in the biological process: regulation of cell shape; regulation of embryonic cell shape; dorsal closure; axon guidance; optic lobe placode development; JNK cascade; imaginal disc fusion, thorax closure; photoreceptor cell morphogenesis; nuclear migration. 43 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; multi-cell-component structure; external compound sense organ; adult segment; peripheral nervous system; nervous system; cell projection; imaginal precursor; rhabdomere; synaptic neuropil subdomain; wing hair; primordium; axon. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Citron-like; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site; Serine/threonine-protein kinase, catalytic domain; Serine/threonine-protein kinase-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1632475_at completely aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of msn. Gene sequence location is 3L:2555775..2586540.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Reis et al., 2010, PLoS Genet. 6(11): e1001206 Carreira et al., 2011, BMC Dev. Biol. 11: 32 Cobreros-Reguera et al., 2010, EMBO Rep. 11(12): 943--949 Alleles msn[S055409] Insertions P{EP}l(3)S055409[S055409] Sequence features BKN50851 BKN28948 HFA08498 BKN51329 HFA07990
FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 62E6-62E7   Limits computationally determined from genome sequence between P{PZ}Spn06911 and P{PZ}l(3)0680306803
Experimentally Determined Cytological Location
Cytogenetic map Notes References 62E5-62E7   (determined by in situ hybridisation)   (Huang and Rubin, 2000) 62E5-62E8   (determined by in situ hybridisation) 62E6--7 (determined by in situ hybridisation)   (Spradling et al., 1999) 62E6-62E7   (determined by in situ hybridisation)   (Paricio et al., 1999) 62E6-62E7   (determined by in situ hybridisation)   (Meister, 1998.2.10) 62E6-62E7   (determined by in situ hybridisation)   (Forjanic et al., 1997) 62E6-62E7   (determined by in situ hybridisation)   (Treisman et al., 1997) 66E6-66E7   (determined by in situ hybridisation)   (Perrimon et al., 1996) 62E5-62E8   62E6--7 62F6--6   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes	msn maps less than 1cM from ecd. (Sorrentino et al., 2002)
Gene Model & Products
Please see the GBrowse view of Dmel\msn for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone appears problematic: incomplete CDS (LD16337)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) msn-RA FBtr0072956  NM_079940   5490   1504 msn-RB FBtr0072957  NM_206252   4578   1200 msn-RC FBtr0072959  NM_206250   4267   1102 msn-RD FBtr0072958  NM_206251   4284   1102 msn-RE FBtr0072960  NM_206249   4104   1042
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein msn-PA   FBpp0072830   162.4   1504   9.82     NP_524679   AAF47658 msn-PB   FBpp0089310   130.3   1200   8.86     NP_995974   AAS64942 msn-PC   FBpp0089312   120.7   1102   8.06     NP_995972   AAS64944 msn-PD   FBpp0089311   120.7   1102   8.06     NP_995973   AAS64945 msn-PE   FBpp0089313   115.4   1042   8.17     NP_995971   AAS64943
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Citron-like (IPR001180) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA247055   AA441414   AA441433   AA736030   AA979568   AE014296 AAF47658   AE014296 AAS64942   AE014296 AAS64943   AE014296 AAS64944   AE014296 AAS64945   AF006653   AI531518   AI533382   AI944625   AQ025110   AQ025126   AQ026296   AQ073313   AW942411   BG637447   BG638730   BI170745   BI212850   BI231531   BI232278   BI232730   BI234317   BI235813   BI355876   BI361482   BI364284   BI365867   BI372041   BI633262   BI633754   BI639143   BP552530   BP558533   BT011116   BT031263   CA803277   CK658012   CL610200   CO152918   CO176778   CO178502   CO182853   CO188109   CO188119   CO188531   CO193299   CO262209   CO264135   CO264357   CO264964   CO272858   CO282938   CO284050   CO285192   CO287556   CO292709   CO294260   CO296090   CO298522   CO303078   CO304299   CO305964   CO310041   CO311028   CO312107   CO312733   CO321502   CO322007   CO325388   CO326103   CO327003   CZ468442   CZ483068   CZ483069   EC060903   EC062952   EC064251   EC067219   EC077446   EC078507   EC080693   EC081826   EC085689   EC085794   EC086250   EC088482   EC090442   EC195255   EC200445   EC202452   EC207496   EC211010   EC212772   EC213817   EC218066   EC223917   EC225063   EC227752   EC232394   EC234099   EC237685   EC240410   EC241665   EC244141   EC244408   EC244666   EC246851   EC249025   EC260072   EC262654   EC264664   G00763   GH941597   GH941705   GH944001   GH949451   GH951353   GH956067   GH956409   GH956848   GH957198   GH958744   GH959451   GH961752   GH965222   GH965329   GH965847   GH966902   GH967470   GH967682   GH968275   GH970356   GH972485   GH973650   GH974163   GH974259
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KV88 Q7KV89 Q7KV90 Q9W002
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0010909    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0010909 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0010909 expression level Developmental Stage   Expression Level embryo 00-02hr    4946 embryo 02-04hr    6980 embryo 04-06hr    4420 embryo 06-08hr    4794 embryo 08-10hr    6208 embryo 10-12hr    6609 embryo 12-14hr    6847 embryo 14-16hr    6983 embryo 16-18hr    4964 embryo 18-20hr    3558 embryo 20-22hr    3744 embryo 22-24hr    3409 larva L1    1606 larva L2    1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6    2724 larva L3 puffstage 7-9    4124 white prepupae new    5417 white prepupae 12hr    9959 white prepupae 24hr    7368 pupae 2d postWPP    4696 pupae 3d postWPP    2171 pupae 4d postWPP    2355 adult male 01day    1726 adult male 05day    1345 adult male 30day    1252 adult female 01day    2885 adult female 05day    3433 adult female 30day    3460 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4946) embryo 02-04hr  (6980) embryo 04-06hr  (4420) embryo 06-08hr  (4794) embryo 08-10hr  (6208) embryo 10-12hr  (6609) embryo 12-14hr  (6847) embryo 14-16hr  (6983) embryo 16-18hr  (4964) embryo 18-20hr  (3558) embryo 20-22hr  (3744) embryo 22-24hr  (3409) larva L1  (1606) larva L2  (1535) larva L3 12hr old  (1317) larva L3 puffstage 1-2  (1002) larva L3 puffstage 3-6  (2724) larva L3 puffstage 7-9  (4124) white prepupae new  (5417) white prepupae 12hr  (9959) white prepupae 24hr  (7368) pupae 2d postWPP  (4696) pupae 3d postWPP  (2171) pupae 4d postWPP  (2355) adult male 01day  (1726) adult male 05day  (1345) adult male 30day  (1252) adult female 01day  (2885) adult female 05day  (3433) adult female 30day  (3460) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4946) embryo 02-04hr  (6980) embryo 04-06hr  (4420) embryo 06-08hr  (4794) embryo 08-10hr  (6208) embryo 10-12hr  (6609) embryo 12-14hr  (6847) embryo 14-16hr  (6983) embryo 16-18hr  (4964) embryo 18-20hr  (3558) embryo 20-22hr  (3744) embryo 22-24hr  (3409) larva L1  (1606) larva L2  (1535) larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6  (2724) larva L3 puffstage 7-9  (4124) white prepupae new  (5417) white prepupae 12hr  (9959) white prepupae 24hr  (7368) pupae 2d postWPP  (4696) pupae 3d postWPP  (2171) pupae 4d postWPP  (2355) adult male 01day  (1726) adult male 05day    1345 adult male 30day    1252 adult female 01day  (2885) adult female 05day  (3433) adult female 30day  (3460) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4946 embryo 02-04hr    6980 embryo 04-06hr    4420 embryo 06-08hr    4794 embryo 08-10hr    6208 embryo 10-12hr    6609 embryo 12-14hr    6847 embryo 14-16hr    6983 embryo 16-18hr    4964 embryo 18-20hr    3558 embryo 20-22hr    3744 embryo 22-24hr    3409 larva L1    1606 larva L2    1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6    2724 larva L3 puffstage 7-9    4124 white prepupae new    5417 white prepupae 12hr    9959 white prepupae 24hr    7368 pupae 2d postWPP    4696 pupae 3d postWPP    2171 pupae 4d postWPP    2355 adult male 01day    1726 adult male 05day    1345 adult male 30day    1252 adult female 01day    2885 adult female 05day    3433 adult female 30day    3460 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4946 embryo 02-04hr    6980 embryo 04-06hr    4420 embryo 06-08hr    4794 embryo 08-10hr    6208 embryo 10-12hr    6609 embryo 12-14hr    6847 embryo 14-16hr    6983 embryo 16-18hr    4964 embryo 18-20hr    3558 embryo 20-22hr    3744 embryo 22-24hr    3409 larva L1    1606 larva L2    1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6    2724 larva L3 puffstage 7-9    4124 white prepupae new    5417 white prepupae 12hr    9959 white prepupae 24hr    7368 pupae 2d postWPP    4696 pupae 3d postWPP    2171 pupae 4d postWPP    2355 adult male 01day    1726 adult male 05day    1345 adult male 30day    1252 adult female 01day    2885 adult female 05day    3433 adult female 30day    3460 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0010909 expression level Developmental Stage   Expression Level embryo 00-02hr    4946 embryo 02-04hr    6980 embryo 04-06hr    4420 embryo 06-08hr    4794 embryo 08-10hr    6208 embryo 10-12hr    6609 embryo 12-14hr    6847 embryo 14-16hr    6983 embryo 16-18hr    4964 embryo 18-20hr    3558 embryo 20-22hr    3744 embryo 22-24hr    3409 larva L1    1606 larva L2    1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6    2724 larva L3 puffstage 7-9    4124 white prepupae new    5417 white prepupae 12hr    9959 white prepupae 24hr    7368 pupae 2d postWPP    4696 pupae 3d postWPP    2171 pupae 4d postWPP    2355 adult male 01day    1726 adult male 05day    1345 adult male 30day    1252 adult female 01day    2885 adult female 05day    3433 adult female 30day    3460 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4946) embryo 02-04hr  (6980) embryo 04-06hr  (4420) embryo 06-08hr  (4794) embryo 08-10hr  (6208) embryo 10-12hr  (6609) embryo 12-14hr  (6847) embryo 14-16hr  (6983) embryo 16-18hr  (4964) embryo 18-20hr  (3558) embryo 20-22hr  (3744) embryo 22-24hr  (3409) larva L1  (1606) larva L2  (1535) larva L3 12hr old  (1317) larva L3 puffstage 1-2  (1002) larva L3 puffstage 3-6  (2724) larva L3 puffstage 7-9  (4124) white prepupae new  (5417) white prepupae 12hr  (9959) white prepupae 24hr  (7368) pupae 2d postWPP  (4696) pupae 3d postWPP  (2171) pupae 4d postWPP  (2355) adult male 01day  (1726) adult male 05day  (1345) adult male 30day  (1252) adult female 01day  (2885) adult female 05day  (3433) adult female 30day  (3460) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4946) embryo 02-04hr  (6980) embryo 04-06hr  (4420) embryo 06-08hr  (4794) embryo 08-10hr  (6208) embryo 10-12hr  (6609) embryo 12-14hr  (6847) embryo 14-16hr  (6983) embryo 16-18hr  (4964) embryo 18-20hr  (3558) embryo 20-22hr  (3744) embryo 22-24hr  (3409) larva L1  1606 larva L2  1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6  (2724) larva L3 puffstage 7-9  (4124) white prepupae new  (5417) white prepupae 12hr  (9959) white prepupae 24hr  (7368) pupae 2d postWPP  (4696) pupae 3d postWPP  2171 pupae 4d postWPP  2355 adult male 01day  1726 adult male 05day    1345 adult male 30day    1252 adult female 01day  (2885) adult female 05day  (3433) adult female 30day  (3460) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4946 embryo 02-04hr    6980 embryo 04-06hr    4420 embryo 06-08hr    4794 embryo 08-10hr    6208 embryo 10-12hr    6609 embryo 12-14hr    6847 embryo 14-16hr    6983 embryo 16-18hr    4964 embryo 18-20hr    3558 embryo 20-22hr    3744 embryo 22-24hr    3409 larva L1    1606 larva L2    1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6    2724 larva L3 puffstage 7-9    4124 white prepupae new    5417 white prepupae 12hr    9959 white prepupae 24hr    7368 pupae 2d postWPP    4696 pupae 3d postWPP    2171 pupae 4d postWPP    2355 adult male 01day    1726 adult male 05day    1345 adult male 30day    1252 adult female 01day    2885 adult female 05day    3433 adult female 30day    3460 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4946 embryo 02-04hr    6980 embryo 04-06hr    4420 embryo 06-08hr    4794 embryo 08-10hr    6208 embryo 10-12hr    6609 embryo 12-14hr    6847 embryo 14-16hr    6983 embryo 16-18hr    4964 embryo 18-20hr    3558 embryo 20-22hr    3744 embryo 22-24hr    3409 larva L1    1606 larva L2    1535 larva L3 12hr old    1317 larva L3 puffstage 1-2    1002 larva L3 puffstage 3-6    2724 larva L3 puffstage 7-9    4124 white prepupae new    5417 white prepupae 12hr    9959 white prepupae 24hr    7368 pupae 2d postWPP    4696 pupae 3d postWPP    2171 pupae 4d postWPP    2355 adult male 01day    1726 adult male 05day    1345 adult male 30day    1252 adult female 01day    2885 adult female 05day    3433 adult female 30day    3460 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0010909    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0010909 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0010909 expression level Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0010909 expression level Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    252.025 Larval Midgut    235.2 Larval Hindgut    194.3 Larval Malpighian Tubules    177.2 Larval Fat Body    107.5 Larval Salivary Gland    59.1 Larval Trachea    203.9 Larval Carcass    147.625 Adult Head    117.9 Adult Eye    65.3 Adult Brain    163.3 Adult Thoracic-Abdominal Ganglion    125 Adult Crop    280.7 Adult Midgut    209.9 Adult Hindgut    200.1 Adult Malpighian Tubules    44.5 Adult Fat Body    111.9 Adult Salivary Gland    95.5 Adult Heart    149.3 Adult VirginFemale Spermatheca    84.5 Adult InseminatedFemale Spermatheca    72.4 Adult Ovary    480.3 Adult Testis    53.1 Adult Male Accessory Gland    105.8 Adult Carcass    113.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    117.9   NA  Eye    65.3   NA  Brain    163.3   252.025  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    125   NA  Crop    280.7   235.2  Midgut    209.9   194.3  Hindgut    200.1   177.2  Malpighian Tubules    44.5   107.5  Fat Body    111.9   59.1  Salivary Gland    95.5   NA  Heart    149.3   203.9  Trachea    NA   NA  VirginFemale Spermatheca    84.5   NA  InseminatedFemale Spermatheca    72.4   NA  Ovary    480.3   NA  Testis    53.1   NA  Male Accessory Gland    105.8   147.625  Carcass    113.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010909 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16973-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4elav.PLu, msn03349 msnD160N.Scer\UAS lethal, with Scer\GAL4elav.PLu, msnD160N.Scer\UAS msn03349 lethal | embryonic stage | maternal effect | poor msn03349 lethal | larval stage | recessive msn03349 lethal | pupal stage msn03349 lethal | pupal stage | recessive msn03349 lethal | recessive msn00817 msn03348 msn03349 msn05221 msn06286 msn06420 msn06683 msn06946 msn10640 msnj1E2 lethal | recessive, with Scer\GAL4[-]: msnEP609 semi-lethal msn03349 msn06946 semi-lethal | recessive msn03349 semi-viable msn03349 Other Phenotypes Allele neuroanatomy defective msn03349 neuroanatomy defective, with Scer\GAL4GMR.PF msnEP549 neuroanatomy defective (with msn102) msn03349 neuroanatomy defective (with msn03349) msn102 planar polarity defective, with Scer\GAL4hs.2sev msnEP549 msnScer\UAS.cSa visible msn102 msn130 msn172 visible, with Scer\GAL4ap-md544 msnEP549 visible, with Scer\GAL4hs.2sev msnEP549 msnScer\UAS.cSa visible | somatic clone msnunspecified wild-type, with Scer\GAL469B msnP656A,P659A.Scer\UAS Phenotype manifest in Allele adult thorax msn03349 axon, with Scer\GAL469B, msn102 msnP656A,P659A.Scer\UAS axon, with Scer\GAL469B, msnP656A,P659A.Scer\UAS msn102 dorsal closure embryo msn172 egg | germline clone msn130 msn172 embryonic/first instar larval cuticle | dorsal msn102 msn172 embryonic/first instar larval cuticle | maternal effect msn03349 embryonic leading edge cell, with Scer\GAL4e22c msnDN.Scer\UAS embryonic leading edge cell, with Scer\GAL4LE msnDN.Scer\UAS embryonic segment | dorsal closure stage msn172 eye, with Scer\GAL4hs.2sev msnEP549 msnScer\UAS.cSa eye disc, with Scer\GAL469B, msn102 msnP656A,P659A.Scer\UAS eye disc, with Scer\GAL469B, msnP656A,P659A.Scer\UAS msn102 eye photoreceptor cell msn102 msn130 msn172 eye photoreceptor cell, with Scer\GAL4hs.2sev msnEP549 eye photoreceptor cell & axon msn03349 eye photoreceptor cell & axon, with Scer\GAL4GMR.PF msnEP549 eye photoreceptor cell & axon | somatic clone msn102 eye photoreceptor cell & growth cone msn03349 eye photoreceptor cell | somatic clone msn102 msn172 filamentous actin | dorsal closure stage msn172 filamentous actin | dorsal closure stage, with Scer\GAL4e22c msnDN.Scer\UAS filamentous actin | dorsal closure stage, with Scer\GAL4LE msnDN.Scer\UAS frontal ganglion msn06683 intersegmental nerve, with Scer\GAL4elav-C155 msnEP549 lamina msn03349 lamina, with Scer\GAL4GMR.PF msnScer\UAS.cSa lamina | somatic clone msn102 lamina neuropil | somatic clone msn102 lamina plexus msn03349 lamina plexus, with Scer\GAL4GMR.PF msnEP549 medulla msn03349 medulla, with Scer\GAL4GMR.PF msnEP549 medulla | somatic clone msn102 microchaeta & scutum, with Scer\GAL4ap-md544 msnEP549 myosin II complex | dorsal closure stage, with Scer\GAL4e22c msnDN.Scer\UAS myosin II complex | dorsal closure stage, with Scer\GAL4LE msnDN.Scer\UAS neuron | somatic clone msnH1135 ommatidial precursor cluster, with Scer\GAL4hs.2sev msnEP549 ommatidial precursor cluster | somatic clone msn102 msn172 ommatidium, with Scer\GAL4hs.2sev msnEP549 msnScer\UAS.cSa ommatidium | somatic clone msn102 msn172 photoreceptor & axon, with Scer\GAL469B, msn102 msnP656A,P659A.Scer\UAS photoreceptor & axon, with Scer\GAL469B, msnP656A,P659A.Scer\UAS msn102 photoreceptor & axon | somatic clone msn102 photoreceptor cell & axon msn03349 photoreceptor cell | precursor & nucleus, with Scer\GAL4unspecified msnDN.Scer\UAS photoreceptor cell | precursor & nucleus, with Scer\GAL4unspecified, msn03349 msnDN.Scer\UAS photoreceptor cell | precursor & nucleus, with Scer\GAL4unspecified, msnDN.Scer\UAS msn03349 photoreceptor cell | precursor & nucleus | somatic clone msn102 photoreceptor cell R1 & axon, with Scer\GAL4GMR.PF msnEP549 msnScer\UAS.cSa photoreceptor cell R1 & axon (with msn102) msn03349 photoreceptor cell R1 & axon (with msn03349) msn102 photoreceptor cell R1 & axon | somatic clone msn102 photoreceptor cell R2 & axon, with Scer\GAL4GMR.PF msnEP549 msnScer\UAS.cSa photoreceptor cell R2 & axon (with msn102) msn03349 photoreceptor cell R2 & axon (with msn03349) msn102 photoreceptor cell R2 & axon | somatic clone msn102 photoreceptor cell R3 & axon, with Scer\GAL4GMR.PF msnEP549 msnScer\UAS.cSa photoreceptor cell R3 & axon (with msn102) msn03349 photoreceptor cell R3 & axon (with msn03349) msn102 photoreceptor cell R3 & axon | somatic clone msn102 photoreceptor cell R4 & axon, with Scer\GAL4GMR.PF msnEP549 msnScer\UAS.cSa photoreceptor cell R4 & axon (with msn102) msn03349 photoreceptor cell R4 & axon (with msn03349) msn102 photoreceptor cell R4 & axon | somatic clone msn102 photoreceptor cell R4 | somatic clone msn102 msn172 photoreceptor cell R5 & axon, with Scer\GAL4GMR.PF msnEP549 msnScer\UAS.cSa photoreceptor cell R5 & axon (with msn102) msn03349 photoreceptor cell R5 & axon (with msn03349) msn102 photoreceptor cell R5 & axon | somatic clone msn102 photoreceptor cell R6 & axon, with Scer\GAL4GMR.PF msnEP549 msnScer\UAS.cSa photoreceptor cell R6 & axon (with msn102) msn03349 photoreceptor cell R6 & axon (with msn03349) msn102 photoreceptor cell R6 & axon | somatic clone msn102 photoreceptor cell R7 & axon, with Scer\GAL4GMR.PF msnEP549 photoreceptor cell R8 & axon, with Scer\GAL4GMR.PF msnEP549 rhabdomere msn102 msn130 msn172 rhabdomere, with Scer\GAL4hs.2sev msnEP549 rhabdomere | somatic clone msn102 msn172 stomodeal nerve msn06683 wing disc | somatic clone msnunspecified wing hair, with Scer\GAL4ap-md544 msnEP549 wing hair | precursor | somatic clone msnunspecified wing hair | somatic clone msnunspecified
Classical Alleles ( 29 )
For All Classical Alleles Show
Allele of msn Class Mutagen Stocks Known lesion msnj1E2 P-element activity 2 -- msn06946 P-element activity 1 -- msn102 loss of function allele X ray 1 Yes msn172 X ray 1 Yes msn5-HA-3063 1 -- msnc03118 piggyBac transposase 1 -- msnEY08051 P-element activity 1 -- msnf00606 piggyBac transposase 1 -- msnMB00430 1 -- msnNP4746 1 -- msn00817 P-element activity 0 -- msn03348 P-element activity 0 -- msn03349 hypomorphic allele - genetic evidence P-element activity 0 -- msn05221 P-element activity 0 -- msn06286 P-element activity 0 -- msn06420 P-element activity 0 -- msn06683 P-element activity 0 -- msn10640 P-element activity 0 -- msn130 X ray 0 Yes msn5-HA-2429 0 -- msnCPTI003908 piggyBac transposase 0 Yes msnEP549 P-element activity 0 Yes msnEP609 P-element activity 0 Yes msnH1135 chemical 0 -- msnNP0687 0 -- msnNP4728 0 -- msnS055409 0 -- msnS138716 P-element activity 0 Yes msnunspecified 0 --
Alleles Carried on Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Allele of msn Class Mutagen Stocks Known lesion msnScer\UAS.cSa in vitro construct - regulatory fusion 2 Yes msnJF03219 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes msnKK108948 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes msn-CT.Scer\UAS in vitro construct - deletion 0 Yes msnD160N.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes msnDN.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes msndsRNA.cCa in vitro construct - RNAi 0 Yes msnF.Scer\UAS.T:Uuuu\Far1 in vitro construct - coding region fusion 0 Yes msnGD1493 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes msnKD.Scer\UAS in vitro construct - amino acid replacement 0 Yes msnMtnA.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes msnP656A,P659A.Scer\UAS in vitro construct - amino acid replacement 0 Yes msnScer\UAS.P\T.T:Ivir\HA1,T:Uuuu\Myr5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes msnΔ332-667.Scer\UAS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Aprt-32 (Spradling et al., 1999, Keegan et al., 2001) Df(3L)Aprt-102 (Keegan et al., 2001) Df(3L)Aprt-148 (Keegan et al., 2001) Df(3L)Aprt-156 (Keegan et al., 2001) Df(3L)h-i22 (BDGP Project Members, 1994-1999) Df(3L)HR370 (BDGP Project Members, 1994-1999) Df(3L)M21 (BDGP Project Members, 1994-1999) In(3)msn102 (Su et al., 1998, Treisman et al., 1997) In(3)msn172 (Su et al., 1998, Treisman et al., 1997)
Not disrupted in	Df(3L)R-G7 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1493} NA P{KK108948} NA P{TRiP.JF03219} NA P{UAS-msn.D160N} NA P{UAS-msn.DN} NA P{UAS-msn.F} NA P{UAS-msn.P656A,P659A} NA P{UAS-msn.S} NA P{UAS-msn.Δ332-667} NA P{UAS-Msn(-CT)} NA P{UAS-Msn(KD)} NA P{UASp-myr-msn} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}msnj1E2 No P{lacW}msnS138716 No P{PZ}msn00817 No P{PZ}msn03348 No P{PZ}msn03349 No P{PZ}msn05221 No P{PZ}msn06286 No P{PZ}msn06420 No P{PZ}msn06683 No P{PZ}msn06946 No P{PZ}msn10640 No insertion of mobile activating element P{EP}l(3)S055409S055409 NA P{EP}msnEP549 NA P{EP}msnEP609 NA P{EPgy2}msnEY08051 NA miscellaneous insertions P{PTT-GC}CC00523 NA P{RS5}msn5-HA-2429 NA P{RS5}msn5-HA-3063 NA PBac{802.P.SVS-2}msnCPTI003908 NA PBac{PB}msnc03118 NA PBac{WH}msnf00606 NA insertion of enhancer trap binary system Mi{ET1}msnMB00430 No P{GawB}msnNP0687 No P{GawB}msnNP4728 No P{GawB}msnNP4746 No
Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Berger et al., 2008) dorsal closure inferred from genetic interaction with bsk AND inferred from genetic interaction with hep AND inferred from mutant phenotype (Su et al., 1998) inferred from mutant phenotype (Koppen et al., 2006, Kadrmas et al., 2004) imaginal disc fusion, thorax closure inferred from mutant phenotype (Kadrmas et al., 2004) JNK cascade inferred from genetic interaction with bsk AND inferred from genetic interaction with hep AND inferred from mutant phenotype AND inferred from sequence or structural similarity (Su et al., 1998) nuclear migration inferred from mutant phenotype (Houalla et al., 2005) optic lobe placode development inferred from mutant phenotype (Houalla et al., 2005) photoreceptor cell morphogenesis inferred from mutant phenotype (Treisman et al., 1997) regulation of cell shape inferred from mutant phenotype (Treisman et al., 1997) regulation of embryonic cell shape inferred from mutant phenotype (Koppen et al., 2006)
Cellular Component
CV term Evidence References
cell cortex inferred from direct assay (Koppen et al., 2006)
Terms Based on Predictions or Assertions ( 12 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) JUN kinase kinase kinase kinase activity traceable author statement (Martin and Wood, 2002) MAP kinase kinase kinase kinase activity inferred from sequence or structural similarity (Su et al., 1998) inferred from sequence or structural similarity with Saccharomyces STE20 (FlyBase, 1992-) non-traceable author statement (Stronach and Perrimon, 2002) protein serine/threonine kinase activity inferred from sequence or structural similarity (Spradling et al., 1999) non-traceable author statement (Su et al., 1998) receptor signaling protein serine/threonine kinase activity non-traceable author statement (Morrison et al., 2000) small GTPase regulator activity inferred from electronic annotation with InterPro:IPR001180 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
activation of MAPKKK activity non-traceable author statement (Su et al., 1998) dorsal closure traceable author statement (Noselli and Agnes, 1999, Van Aelst and Symons, 2002, Harden, 2002) establishment of planar polarity non-traceable author statement (Mlodzik, 2002) JNK cascade non-traceable author statement (Noselli and Agnes, 1999) oogenesis non-traceable author statement (Treisman et al., 1997) protein phosphorylation inferred from sequence or structural similarity (Spradling et al., 1999) inferred from sequence or structural similarity with ninaC AND inferred from sequence or structural similarity with Saccharomyces CDC15 (Treisman et al., 1997) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions msn allele Gene References msn102 bsk (Su et al., 1998) dock (Su et al., 2000) dsh (Paricio et al., 1999) hep (Su et al., 1998) Jra (Su et al., 1998) Rac1 (Stronach and Perrimon, 2002) tkv (Su et al., 1998) msn172 dsh (Paricio et al., 1999) egr (Igaki et al., 2002) Jra (Su et al., 1998) Tak1 (Mihaly et al., 2001) tkv (Su et al., 1998) msn03349 BicD (Houalla et al., 2005) dock (Ruan et al., 1999) msnDN.Scer\UAS BicD (Houalla et al., 2005) Lis-1 (Houalla et al., 2005) msnEP549 bif (Ruan et al., 2002) Cdc42 (Ruan et al., 2002) dock (Ruan et al., 1999) Nrt (Ruan et al., 2002) msnEP609 ksr::tor (Huang and Rubin, 2000) Ras85D (Huang and Rubin, 2000) unspecified bsk (Paricio et al., 1999) Egfr (Read et al., 2005) H (Mueller et al., 2005) hep (Paricio et al., 1999) ics (Kadrmas et al., 2004) Jra (Paricio et al., 1999) ksr (Huang and Rubin, 2000) Ppt1 (Buff et al., 2007) Ret (Read et al., 2005) slpr (Polaski et al., 2006) stck (Kadrmas et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68789 DroID - A comprehensive database of gene and protein interactions. • FBgn0010909 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44030
Orthologs
Genome-wide drosophilid orthologs	Dana\GF25017 Dere\GG14510 Dgri\GH16388 Dmoj\GI12471 Dper\GL22452 Dpse\GA24328 Dsec\GM14115 Dsim\GD13385 Dvir\GJ12373 Dwil\GK12652 Dyak\GE21701
Curated drosophilid orthologs	Dpse\GA14247
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006730-PA Aedes aegypti (mosquito) AGAP006340-PA Anopheles gambiae (mosquito) 124841 Branchiostoma floridae (lancelet) BGIBMGA007730-PA Bombyx mori (silk worm) ENSBTAP00000006458 Bos taurus (bovine) CBP33647 Caenorhabditis briggsae (nematode) ENSCAFP00000023157 Canis familiaris (dog) ENSCINP00000010913 Ciona intestinalis (sea squirt) ENSCPOP00000010460 Cavia porcellus (guinea pig) 134455 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000013881 Ciona savignyi (sedentary tunicate) 194254 Capitella spl (polychaete worm) ENSDARP00000051235 Danio rerio (zebra fish) ENSECAP00000007446 Equus caballus (horse) ENSGACP00000020019 Gymnopilus aculeatus (mushroom) ENSGALP00000014575 Gallus gallus (chicken) 156036 Hypsipyla robusta (cedar shoot borer) ENSP00000313644 Homo sapiens (human) 214738 Lottia gigantea (owl limpet) 29957 Monocosta brevicollis (choanoflagellate) ENSMODP00000006774 Monodelphis domestica (gray opossum) ENSMMUP00000014167 Macaca mulatta (rhesus monkey) ENSMUSP00000072091 Mus musculus (house mouse) ENSOANP00000018449 Ornithorynchus anatinus (platypus) ENSORLP00000010011 Oryzias latipes (japanese killifish) PHUM005152-PA Pediculus humanus (human body louse) PP10990 Pristionchus pacificus (nematode) ENSPPYP00000013498 Pongo pygmaeus (orangutan) 136369 Phytophthora sojae (root rot pathogen) ENSPTRP00000021060 Pan troglodytes (chimpanzee) ENSRNOP00000048832 Rattus norvegicus (norway rat) YHR102W Saccharomyces cerevisiae (budding yeast) Smp_163420.1 Schistosoma mansoni (trematode parasite) XP_001186054.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001193111.1 Strongylocentrotus purpuratus (purple sea urchin) XP_968527.2 Tribolium castaneum (red flour beetle) ENSTNIP00000003547 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000041760 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000009417 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13799 NV18625 LH12881 ISCW023156 FBgn0238949 FBgn0214661 FBgn0199618 FBgn0185099 FBgn0169044 JGI_V11_301634 FBgn0245725 FBgn0160044 FBgn0135228 FBgn0123859 FBgn0106769 FBgn0102009 CPIJ011908 BGIBMGA007730 GB11839 AGAP006340 AAEL006730 PHUM076200 GLEAN_14568
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	5946 w*; P{UAS-msn.S}2 28791 y1 v1; P{TRiP.JF03219}attP2 5945 w*; msn102 P{neoFRT}80B/TM6B 5947 w*; msn172 P{neoFRT}80B/TM6B 22796 y1 w67c23; Mi{ET1}msnMB00430 10162 y1 w1118; P{lacW}msnj1E2/TM3, Sb1
Harvard	- PBac{PB}msnc03118 - PBac{WH}msnf00606
Kyoto	108496 w*; P{UAS-msn.S}2 125790 w1118; P{RS5}msn5-HA-3063 113512 w*; P{GawB}msnNP4746 / TM3, Sb1 Ser1
VDRC	v101517 P{KK108948}VIE-260B
	More stocks available...
Genomic Clones ( 1 )
	BACR27A03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 122 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD16337 LD34191
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM10814 GM32681 LD06193 LD16364 LP14490 RE12316 RE18095 RE22407 RE28324 RE28851 RE30007 RE31748 RE45711 RE49360 RE51260 RE57474 SD02593 SD05170 SD15452 SD21604 SD27307 SD27909
Other clones	9448 16494 28157 30187 32292 45822 46532 58356 59969 60863 60898 65805 67817 69369 76496 80326 97872 104329 104689 120203 122023 122168 147289 175395 180297 190275 194539 214569.23 521933 523972 bs02g10 CK01.7A.C10 CK01.44D.B1 CK01.44D.C2 CK01.51C.H3 CK01.71D.F1 CK01.82D.F6 CK01.97D.F5 EC24144 EC35551 EK037404 EK037431 EK040830 EK050940 EK056916 EK066155 EK084703 EK108555 EK137605 EK138739 EK141044 EK161816 EK165127 EK168458 EK173162 EK185812 EK186964 EK196364 EK200809 EK206441 EK212652 EK220316 EK223202 EK227883 EK229818 EK239849 EK244306 EK249913 EN00822 EP03236 EP05434 ESG011b.E7_F04 FGM219C07 FGM315E08 RP004025102 RP004063560 RP004070040 RP004079115 RP004086195 RP004158530 RP004184892 RP004246836 RP004263217 RP004265880 RP004367039 RP004368711 RP004378322 RP004396252 RP004429112 RP004457000 RP004464418 RP004467063 RP004474095 RP004486671 RP004493570 RP004495030 RP004531468 RP004559137
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0010909 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44030
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: msn Nik (Manning, 2000.8.8) Source for merge of: Nik CG16973 (Misra, 2000.8.9)
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) msn acts through bsk in epithelial planar polarity (EPP) signalling. (Paricio et al., 1999) msn acts downstream of fz and dsh in the epithelial planar polarity (EPP) signalling pathway in eyes and wings. (Paricio et al., 1999) msn is required for the proper targetting of the R1-R6 photoreceptor cell growth cones in the developing visual system. (Ruan et al., 1999) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) msn functions upstream of hep and bsk to stimulate dorsal closure in the embryo. (Su et al., 1998) Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell dysregulation. (Braun et al., 1997) msn is required for the normal shape and orientation of photoreceptor cells and is essential for oogenesis. msn may act in a signal transduction pathway leading to cytoskeletal rearrangements. (Treisman et al., 1997) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. (Perrimon et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079940 NM_206249 NM_206250 NM_206251 NM_206252 RefSeq (Proteins) • NP_524679 NP_995971 NP_995972 NP_995973 NP_995974 DDBJ / EMBL / GenBank • AA441414 AA979568 AAF47658 AAS64942 AAS64943 AAS64944 AAS64945 AF006653 AI944625 AQ025110 AQ025126 AQ026296 AQ073313 AW942411 BG638730 CL610200 CZ468442 CZ483068 CZ483069 G00763 Entrez Gene - A searchable database of RefSeq genes. • 44030 UniProtKB/TrEMBL • Q7KV88 Q7KV89 Q7KV90 Q9W002
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Citron-like (IPR001180) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68789 DroID - A comprehensive database of gene and protein interactions. • FBgn0010909 DRSC - Results from RNAi screens. • FBgn0010909 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010909 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG16973-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010909 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 450712 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44030 InParanoid A subset of ortholog calls from InParanoid. • AAEL006730-PA Aedes aegypti (mosquito) AGAP006340-PA Anopheles gambiae (mosquito) 124841 Branchiostoma floridae (lancelet) BGIBMGA007730-PA Bombyx mori (silk worm) ENSBTAP00000006458 Bos taurus (bovine) CBP33647 Caenorhabditis briggsae (nematode) ENSCAFP00000023157 Canis familiaris (dog) ENSCINP00000010913 Ciona intestinalis (sea squirt) ENSCPOP00000010460 Cavia porcellus (guinea pig) 134455 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000013881 Ciona savignyi (sedentary tunicate) 194254 Capitella spl (polychaete worm) ENSDARP00000051235 Danio rerio (zebra fish) ENSECAP00000007446 Equus caballus (horse) ENSGACP00000020019 Gymnopilus aculeatus (mushroom) ENSGALP00000014575 Gallus gallus (chicken) 156036 Hypsipyla robusta (cedar shoot borer) ENSP00000313644 Homo sapiens (human) 214738 Lottia gigantea (owl limpet) 29957 Monocosta brevicollis (choanoflagellate) ENSMODP00000006774 Monodelphis domestica (gray opossum) ENSMMUP00000014167 Macaca mulatta (rhesus monkey) ENSMUSP00000072091 Mus musculus (house mouse) ENSOANP00000018449 Ornithorynchus anatinus (platypus) ENSORLP00000010011 Oryzias latipes (japanese killifish) PHUM005152-PA Pediculus humanus (human body louse) PP10990 Pristionchus pacificus (nematode) ENSPPYP00000013498 Pongo pygmaeus (orangutan) 136369 Phytophthora sojae (root rot pathogen) ENSPTRP00000021060 Pan troglodytes (chimpanzee) ENSRNOP00000048832 Rattus norvegicus (norway rat) YHR102W Saccharomyces cerevisiae (budding yeast) Smp_163420.1 Schistosoma mansoni (trematode parasite) XP_001186054.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001193111.1 Strongylocentrotus purpuratus (purple sea urchin) XP_968527.2 Tribolium castaneum (red flour beetle) ENSTNIP00000003547 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000041760 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000009417 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/mishap1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44030 modMine - Data generated by the modENCODE project. • FBgn0010909 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13799 NV18625 LH12881 ISCW023156 FBgn0238949 FBgn0214661 FBgn0199618 FBgn0185099 FBgn0169044 JGI_V11_301634 FBgn0245725 FBgn0160044 FBgn0135228 FBgn0123859 FBgn0106769 FBgn0102009 CPIJ011908 BGIBMGA007730 GB11839 AGAP006340 AAEL006730 PHUM076200 GLEAN_14568
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	6286 (Harvie et al., 1998) CG16973 (Morrison et al., 2000, Manning, 2000.8.8, Carrera et al., 2008, Keleman et al., 2009.8.5, Perkins et al., 2009, Berger et al., 2008) DMSN (Chen et al., 2004) HGK (Balakireva et al., 2007) JNKKKK (Wech and Nagel, 2005) l(3)03349   l(3)6286 (Harvie, 1997.6.4) l(3)06946 (Braun et al., 1997, Forjanic et al., 1997, ) l(3)j1E2 (BDGP Project Members, 1994-1999, ) MESR5 (Huang and Rubin, 2000) msm (Agnes and Noselli, 1999) MSN (Xia and Karin, 2004) Msn (Kockel et al., 2001, Houalla and Rao, 2007, Gonda and Stronach, 2007, Buszczak et al., 2007, Findlay et al., 2007, Lee et al., 2006, Ribeiro et al., 2010) msn (Tyler et al., 2007, Walther and Pichaud, 2007, Koppen et al., 2006, Delaney et al., 2006, Kadrmas et al., 2004, Balakireva et al., 2007, Kaneko et al., 2011, Tiwari and Roy, 2009, Tokusumi et al., 2009, Polaski et al., 2006, Wech and Nagel, 2005, Balakireva et al., 2006, Bakal et al., 2008, Pearson et al., 2009, Carrera et al., 2008, Carreira et al., 2009, Tokusumi et al., 2009, Cook et al., 2010.2.12, Djiane and Mlodzik, 2010, Avet-Rochex et al., 2010, Yavari et al., 2010, Garlena et al., 2010, Mattila et al., 2005, Kim and McGinnis, 2011, Cobreros-Reguera et al., 2010, Reis et al., 2010) NIK (Morrison et al., 2000) Nik   unnamed (Treisman and Rubin, 1996)
Name Synonym	Mishappen (Wang et al., 1999, Suganuma et al., 2010) misshapen (Treisman et al., 1997, Walther and Pichaud, 2007, Korey et al., 2007, Wu et al., 2008, Wech and Nagel, 2005, Link et al., 2007, Fiehler and Wolff, 2008, Findlay et al., 2007, Babcock et al., 2009, Carreira et al., 2009, Tokusumi et al., 2009, Tokusumi et al., 2009, Berger et al., 2008, Ferguson et al., 2009, Yavari et al., 2010, Garlena et al., 2010, Mattila et al., 2005, Kim and McGinnis, 2011) Misshapen (Koppen et al., 2006, Kadrmas et al., 2004, Buff et al., 2007, Llense and Martín-Blanco, 2008, Lee et al., 2006, Ribeiro et al., 2010) NIK (Lee et al., 2006)
Secondary FlyBase IDs
	FBgn0010797 FBgn0024520 FBgn0035351
References ( 151 )
Generate a list of	All references relating to this gene ( 151 )
List References by type	Research paper  ( 78 ) Supplementary material  ( 8 ) Review  ( 21 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 18 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 14 )
	Brooks et al., 2011, Genome Res. 21(2): 193--202 Conservation of an RNA regulatory map between Drosophila and mammals. [FBrf0213079] Carreira et al., 2011, BMC Dev. Biol. 11: 32 Genetic basis of wing morphogenesis in Drosophila: sexual dimorphism and non-allometric effects of shape variation. [FBrf0214273] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Kaneko et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(27): 11127--11132 Smad inhibition by the Ste20 kinase Misshapen. [FBrf0214278] Kim and McGinnis, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(2): 650--655 Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier. [FBrf0214191] Avet-Rochex et al., 2010, BMC Dev. Biol. 10: 65 An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. [FBrf0211137] Cobreros-Reguera et al., 2010, EMBO Rep. 11(12): 943--949 The Ste20 kinase misshapen is essential for the invasive behaviour of ovarian epithelial cells in Drosophila. [FBrf0212408] Djiane and Mlodzik, 2010, PLoS ONE 5(6): e11228 The Drosophila GIPC homologue can modulate myosin based processes and planar cell polarity but is not essential for development. [FBrf0211141] Garlena et al., 2010, J. Cell Sci. 123(18): 3177--3188 Regulation of mixed-lineage kinase activation in JNK-dependent morphogenesis. [FBrf0211702] Lesch et al., 2010, Genetics 186(3): 943--957 A Targeted UAS-RNAi Screen in Drosophila Larvae Identifies Wound Closure Genes Regulating Distinct Cellular Processes. [FBrf0212282] Reis et al., 2010, PLoS Genet. 6(11): e1001206 A buoyancy-based screen of Drosophila larvae for fat-storage mutants reveals a role for sir2 in coupling fat storage to nutrient availability. [FBrf0212335] Ribeiro et al., 2010, Mol. Cell 39(4): 521--534 Combined Functional Genomic and Proteomic Approaches Identify a PP2A Complex as a Negative Regulator of Hippo Signaling. [FBrf0211636] Suganuma et al., 2010, Cell 142(5): 726--736 The ATAC acetyltransferase complex coordinates MAP kinases to regulate JNK target genes. [FBrf0214172] Yavari et al., 2010, Dev. Cell 19(1): 54--65 Role of Lipid Metabolism in Smoothened Derepression in Hedgehog Signaling. [FBrf0211286] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...