Gene Dmel\Sas-4

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General Information

Symbol

Dmel\Sas-4

Species

D. melanogaster

Name

Sas-4

Annotation symbol

CG10061

Feature type

protein_coding_gene

FlyBase ID

FBgn0011020

Created / Updated

2003-12-01/2007-06-14

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

84C6-84C7

Sequence location

3R:2,977,076..2,979,886 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Sas-4 (CG10061, FBgn0011020). It has the cytological map location 84C6-84C7. Its sequence location is 3R:2977076..2979886. Its molecular function is unknown. It is involved in the biological processes: asymmetric cell division; centriole replication; centrosome organization and biogenesis; flagellum biogenesis; cilium biogenesis; locomotion; centrosome duplication; mitotic spindle organization and biogenesis. 5 alleles are reported. The phenotypes of these alleles are annotated with: centriole; centrosome; cilium; flagellum; neuroblast. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84C6-84C7

Limits computationally determined from genome sequence between P{lacW}l(3)L2100^L2100 and P{EP}EP3060^EP3060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84C4-84C6

(determined by in situ hybridisation)

(Spradling et al., 1999)

84C4-84C6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Sas-4 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Sas-4-RA

FBtr0081569

NM_141444

2760

901

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Sas-4-PA

FBpp0081088

103.3

901

7.79

NP_649701

AAF54053

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

T-complex 10/CenJ, C-terminal (IPR009852)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VI72

Maps to

Does NOT map to

Identified with

GM22640

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8459

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011020

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Sas-4^s2214

lethal | adult stage

Sas-4^s2214

Other Phenotypes

Allele

mating defective

Sas-4^s2214

uncoordinated

Sas-4^s2214

mitotic cell cycle defective

Sas-4^s2214

Sterility

Allele

male sterile

Sas-4^s2214

Phenotype manifest in

Allele

centriole

Sas-4^s2214

centrosome

Sas-4^s2214

cilium

Sas-4^s2214

flagellum

Sas-4^s2214

neuroblast

Sas-4^s2214

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Sas-4	Class	Mutagen	Stocks	Known lesion
Sas-4^s2214	loss of function	P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Sas-4	Mutagen	Stocks	Known lesion
Sas-4^GD6852	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Sas-4^{MtnA.C.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
Sas-4^{MtnA.N.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
Sas-4^{Ubi.T:Avic\GFP}	in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)Antp-A41

Useful duplication

Dp(3;3)D1

Disrupted in

Df(3R)Antp17

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3R)BSC221

(Gresens and Cook, 2006.12.5)

Df(3R)ED5221

(Roote, 2004.7.20)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6852}

characterization construct

P{Ubi-Sas-4-GFP}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}Sas-4^s2214

Yes

insertion of mobile activating element

P{EPgy2}EY00044

P{EPgy2}EY05035

Related Comments

Please look at the allele reports for the complete phenotype data

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

asymmetric cell division