FB2020_01, released Feb 12, 2020

Gene: Dmel\dia

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General Information
Symbol
Dmel\dia
Species
D. melanogaster
Name
diaphanous
Annotation Symbol
CG1768
Feature Type
protein_coding_gene
FlyBase ID
FBgn0011202
Gene Model Status
Current
Stock Availability
22 publicly available
Gene Snapshot
diaphanous (dia) encodes the sole Dia-class formin in Drosophila. It nucleates actin filaments de novo and acts as a processive actin polymerase, stimulating actin addition at the barbed end. It plays an essential role in cytokinesis and also regulates cell shape change and protrusion. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

38E.16, l(2)k07135

Key Links
Genomic Location
Cytogenetic map
38E7-38E8
Sequence location
2L:20,758,144..20,768,053 [+]
Recombination map

2-54

RefSeq locus
NT_033779 REGION:20758144..20768053
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the formin homology family. Diaphanous subfamily. (P48608)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
phosphatidylinositol-4,5-bisphosphate binding; protein binding; Rho GTPase binding
Predictions / Assertions
actin binding
Summaries
Protein Function (UniProtKB)
Required for cytokinesis in both mitosis and meiosis. Has a role in actin cytoskeleton organization and is essential for many, if not all, actin-mediated events involving membrane invagination. May serve as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle. Possible component of the contractile ring or may control its function.
(UniProt, P48608)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\dia or the JBrowse view of Dmel\dia for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
dia-RA
FBtr0081410
NM_057633
4743
1091
dia-RB
FBtr0081411
NM_165341
4465
1091
dia-RC
FBtr0305960
NM_001259183
1775
130
dia-RD
FBtr0305961
NM_001259184
3797
1098
dia-RE
FBtr0335145
NM_001273711
5260
1091
Additional Transcript Data and Comments
Reported size (kB)

4.8, 4.4 (northern blot)

(Castrillon and Wasserman, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
dia-PA
FBpp0080940
123.2
1091
8.25
NP_476981
AAF53922
dia-PB
FBpp0080941
123.2
1091
8.25
NP_724285
AAN11087
dia-PC
FBpp0297102
14.5
130
10.05
NP_001246112
AFH03786
dia-PD
FBpp0297103
124.0
1098
8.51
NP_001246113
AFH03787
dia-PE
FBpp0307144
123.2
1091
8.25
NP_001260640
AGB93175
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1091 aa isoforms: dia-PA, dia-PB, dia-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

1091 (aa); 123 (kD predicted)

(Castrillon and Wasserman, 1994)
Comments
External Data
Domain

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).

(UniProt, P48608)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dia using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (40 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
phosphatidylinositol-4,5-bisphosphate binding
inferred from mutant phenotype
(Rousso et al., 2013)
protein binding
inferred from physical interaction with FLYBASE:chic; FB:FBgn0000308
(Webb et al., 2009)
inferred from physical interaction with FLYBASE:Apc2; FB:FBgn0026598
(Webb et al., 2009)
inferred from physical interaction with FLYBASE:Eb1; FB:FBgn0027066
(Webb et al., 2009)
inferred from physical interaction with FLYBASE:Cip4; FB:FBgn0035533
(Yan et al., 2013)
Rho GTPase binding
inferred from mutant phenotype
(Rousso et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
actin binding
inferred from electronic annotation with InterPro:IPR010472, InterPro:IPR010473
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (27 terms)
Terms Based on Experimental Evidence (27 terms)
CV Term
Evidence
References
actin cytoskeleton reorganization
inferred from mutant phenotype
(Abreu-Blanco et al., 2014)
actin filament organization
inferred from mutant phenotype
(Großhans et al., 2005, Afshar et al., 2000)
actin nucleation
inferred from direct assay
(Yan et al., 2013)
actomyosin contractile ring assembly
inferred from mutant phenotype
(Giansanti et al., 2004)
apical constriction
inferred from mutant phenotype
(Chung et al., 2017, Bertet et al., 2009)
assembly of apicomedial cortex actomyosin
inferred from direct assay
(Chung et al., 2017)
cellularization
inferred from mutant phenotype
(Großhans et al., 2005, Afshar et al., 2000)
maintenance of protein location in cell
inferred from mutant phenotype
(Dean et al., 2005)
meiosis I cytokinesis
inferred from mutant phenotype
(Giansanti et al., 2004)
meiosis II cytokinesis
inferred from mutant phenotype
(Giansanti et al., 2004)
melanotic encapsulation of foreign target
inferred from mutant phenotype
(Howell et al., 2012)
mitotic cell cycle, embryonic
inferred from mutant phenotype
(Afshar et al., 2000)
mitotic cytokinesis
inferred from mutant phenotype
(Dean et al., 2005, Echard et al., 2004, Eggert et al., 2004, Rogers et al., 2003)
open tracheal system development
inferred from mutant phenotype
(Matusek et al., 2006)
plasma membrane tubulation
inferred from mutant phenotype
(Yan et al., 2013)
pole cell formation
inferred from mutant phenotype
(Afshar et al., 2000)
positive regulation of actin filament polymerization
inferred from direct assay
(Yan et al., 2013)
positive regulation of wound healing
inferred from mutant phenotype
(Abreu-Blanco et al., 2014)
protein localization
inferred from mutant phenotype
(Yan et al., 2013, Afshar et al., 2000)
protein secretion
inferred from mutant phenotype
(Massarwa et al., 2009)
pseudocleavage involved in syncytial blastoderm formation
inferred from mutant phenotype
(Webb et al., 2009)
regulation of actomyosin structure organization
inferred from mutant phenotype
(Homem and Peifer, 2008)
regulation of embryonic cell shape
inferred from mutant phenotype
(Bertet et al., 2009, Homem and Peifer, 2008)
regulation of filopodium assembly
inferred from mutant phenotype
(Homem and Peifer, 2009)
salivary gland morphogenesis
inferred from mutant phenotype
(Chung et al., 2017)
sensory perception of sound
inferred from mutant phenotype
(Schoen et al., 2010, Cosetti et al., 2008)
spindle assembly
inferred from mutant phenotype
(Giansanti et al., 2004)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
protein localization
non-traceable author statement
(Gonzalez and Bornens, 2000)
Cellular Component (9 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
colocalizes_with actin filament
inferred from direct assay
(Webb et al., 2009)
apical part of cell
inferred from direct assay
(Massarwa et al., 2009, Corrigall et al., 2007)
colocalizes_with apical plasma membrane
inferred from direct assay
(Rousso et al., 2013)
cell cortex
inferred from direct assay
(Homem and Peifer, 2008)
cleavage furrow
inferred from direct assay
(Yan et al., 2013, Homem and Peifer, 2008)
colocalizes_with cytoneme
inferred from direct assay
(Roy et al., 2014)
cytoplasm
inferred from direct assay
(Yan et al., 2013)
protein-containing complex
inferred from physical interaction with FLYBASE:Apc2; FB:FBgn0026598
(Webb et al., 2009)
vesicle membrane
inferred from direct assay
(Yan et al., 2013)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14 -- 17
embryonic/larval trachea
(Massarwa et al., 2009)
embryonic/larval hindgut
(Massarwa et al., 2009)
embryonic/larval Malpighian tubule
(Massarwa et al., 2009)
embryonic/larval salivary gland
(Massarwa et al., 2009)
embryonic/larval proventriculus
(Massarwa et al., 2009)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
(Castrillon and Wasserman, 1994)
adult stage | male
(Castrillon and Wasserman, 1994)
Additional Descriptive Data

dia transcript is expressed predominantly in tubular organs, including trachea, salivary glands, Malpighian tubules, the hindgut and the proventriculus, starting at embryonic stage 14. Transcript is localized to the apical side, facing the tube lumen.

(Massarwa et al., 2009)

dia transcripts are expressed in both males and females and are not germline specific.

(Castrillon and Wasserman, 1994)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14 -- 17
embryonic/larval trachea
(Massarwa et al., 2009)
embryonic/larval hindgut
(Massarwa et al., 2009)
embryonic/larval Malpighian tubule
(Massarwa et al., 2009)
embryonic/larval salivary gland
(Massarwa et al., 2009)
embryonic/larval proventriculus
(Massarwa et al., 2009)
third instar larval stage
embryonic/larval neuromuscular junction
(Pawson et al., 2008)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

dia protein is apically localized in tubular organs.

(Massarwa et al., 2009)

dia protein is found pre- and postsynaptically at the third instar larval neuromuscular junction.

(Pawson et al., 2008)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with actin filament
inferred from direct assay
(Webb et al., 2009)
apical part of cell
inferred from direct assay
(Massarwa et al., 2009, Corrigall et al., 2007)
colocalizes_with apical plasma membrane
inferred from direct assay
(Rousso et al., 2013)
cell cortex
inferred from direct assay
(Homem and Peifer, 2008)
cleavage furrow
inferred from direct assay
(Yan et al., 2013, Homem and Peifer, 2008)
colocalizes_with cytoneme
inferred from direct assay
(Roy et al., 2014)
cytoplasm
inferred from direct assay
(Yan et al., 2013)
protein-containing complex
inferred from physical interaction with FLYBASE:Apc2; FB:FBgn0026598
(Webb et al., 2009)
vesicle membrane
inferred from direct assay
(Yan et al., 2013)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\dia in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 23 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of dia
Transgenic constructs containing regulatory region of dia
Deletions and Duplications ( 20 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with Df(2L)DS9)
lethal | embryonic stage | non-rescuable maternal effect | recessive
lethal | maternal effect | recessive
lethal | recessive
lethal - all die before end of embryonic stage, with Scer\GAL4e22c
lethal - all die before end of P-stage, with Scer\GAL4GMR.PU
lethal - all die before end of pupal stage, with Scer\GAL4NP0761
lethal - all die before end of pupal stage | recessive
partially lethal - majority die, with Scer\GAL4NP1046
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile | recessive
fertile
male sterile
male sterile | recessive
Other Phenotypes
Allele
auditory perception defective, with Scer\GAL4JO15
auditory perception defective, with Scer\GAL4NP1046
auditory perception defective, with Scer\GAL4NP1245
auditory perception defective, with Scer\GAL4NP6303
auditory perception defective | dominant
cell migration defective | embryonic stage, with Scer\GAL4fkh.PH
cell shape defective, with Scer\GAL4pnr.PU
cell shape defective | embryonic stage 11, with Scer\GAL4fkh.PH
cell shape defective | extended germ band stage
cytokinesis defective | male (with Df(2L)TW84)
cytokinesis defective | male | recessive
cytokinesis defective | recessive
cytokinesis defective | somatic clone
decreased cell number | spermatogenesis
decreased occurrence of cell division | spermatogenesis
immune response defective | larval stage, with Scer\GAL4Hml.PG
meiotic cell cycle defective | male (with Df(2L)TW84)
meiotic cell cycle defective | male | recessive
mitotic cell cycle defective | male (with Df(2L)TW84)
mitotic cell cycle defective | male | recessive
neoplasia | larval stage, with Scer\GAL430A
neoplasia | larval stage, with Scer\GAL4dpp.PU
neuroanatomy defective, with Scer\GAL4elav-C155
neuroanatomy defective | larval stage
neurophysiology defective | dominant
size defective | larval stage, with Scer\GAL4dpp.PU
some die during embryonic stage, with Scer\GAL4e22c
some die during pupal stage, with Scer\GAL4NP0761
some die during pupal stage, with Scer\GAL4pnr-MD237
some die during pupal stage | recessive
visible, with Scer\GAL4en.PU
visible, with Scer\GAL4pnr-MD237
visible | adult stage, with Scer\GAL4GMR.PU
wild-type
wound healing defective | embryonic stage, with Scer\GAL4VP16.mat.αTub67C
Phenotype manifest in
Allele
actin filament & embryonic/larval tracheal system
actin filament & embryonic/larval tracheal system, with Scer\GAL4btl.PS
actin filament | embryonic stage | germline clone
air sac primordium | third instar larval stage, with Scer\GAL4btl.PS
amnioserosa, with Scer\GAL4c381
amnioserosa, with Scer\GAL4en-e16E
amnioserosa | maternal effect (with dia2)
amnioserosa | maternal effect (with dia5)
border follicle cell, with Scer\GAL4slbo.2.6
bract | somatic clone
centrosome | embryonic stage | germline clone
commissure, with Scer\GAL4elav-C155
connective, with Scer\GAL4elav-C155
contractile ring & meiotic cell cycle | male
contractile ring & meiotic cell cycle | male (with Df(2L)TW84)
cyst cell
cytoskeleton | embryonic stage | germline clone
denticle belt | germline clone
ectoderm | extended germ band stage
egg chamber
egg chamber | somatic clone
embryo | blastoderm stage | germline clone
embryo | dorsal closure stage | germline clone
embryo | embryonic cycle 11 | maternal effect
embryo | gastrula stage | germline clone
embryonic/first instar larval cuticle | germline clone
embryonic/larval dorsal trunk, with Scer\GAL4btl.PS
embryonic/larval dorsal trunk, with Scer\GAL4en-e16E
embryonic/larval dorsal trunk, with Scer\GAL4prd.RG1
embryonic epidermis, with Scer\GAL4en-e16E
embryonic epidermis, with Scer\GAL4prd.RG1
embryonic head | germline clone
embryonic leading edge cell, with Scer\GAL4en-e16E
epidermal cell, with Scer\GAL4en-e16E
epithelial cell | somatic clone, with Scer\GAL4Act5C.PI
epithelium | somatic clone
external sensory organ precursor cell, with Scer\GAL4pnr.PU
eye
eye, with Scer\GAL4GMR.PU
filamentous actin, with Scer\GAL4slbo.2.6
filopodium
filopodium, with Scer\GAL4en-e16E
filopodium | ectopic, with Scer\GAL4en-e16E
follicle cell
follicle cell | somatic clone
furrow canal | embryonic stage 4 | maternal effect
furrow canal | germline clone
fusion competent cell, with Scer\GAL4sns.PK
ganglion mother cell | larval stage
imaginal disc
lamellipodium
lamellipodium, with Scer\GAL4en-e16E
larval central nervous system & neuroblast
larval neuroblast | larval stage
male germline cell | spermatogenesis
mesothoracic tergum, with Scer\GAL4pnr-MD237
mesothoracic tergum | pupal stage | somatic clone
mesothoracic tergum | pupal stage | somatic clone, with Scer\GAL4FRT.Act5C
microtubule | embryonic stage | germline clone
morphogenetic furrow | somatic clone
Nebenkern
Nebenkern (with Df(2L)TW84)
nucleus | embryonic stage | germline clone
nucleus | larval stage, with Scer\GAL430A
organism | dorsal closure stage, with Scer\GAL4c381
organism | dorsal closure stage | maternal effect (with dia2)
organism | dorsal closure stage | maternal effect (with dia5)
pole bud | germline clone
pole cell | germline clone
presumptive embryonic/larval central nervous system, with Scer\GAL4elav-C155
salivary gland body primordium | embryonic stage 11, with Scer\GAL4fkh.PH
salivary gland body primordium | embryonic stage 14, with Scer\GAL4fkh.PH
spermatid
spermatid & nucleus | supernumerary
spermatid & nucleus | supernumerary (with Df(2L)TW84)
spermatocyte
spindle & meiotic cell cycle | male
spindle & meiotic cell cycle | male (with Df(2L)TW84)
testis
tormogen cell | somatic clone
tracheal fusion cell, with Scer\GAL4btl.PS
ventral furrow | non-rescuable maternal effect
ventral thoracic disc, with Scer\GAL4dpp.PU
wing, with Scer\GAL4en.PU
wing disc, with Scer\GAL430A
wing disc, with Scer\GAL4dpp.PU
wing hinge primordium, with Scer\GAL430A
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (13)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\DIAPH3
13 of 15
Yes
Yes
Hsap\DIAPH1
12 of 15
No
Yes
Hsap\DIAPH2
12 of 15
No
Yes
Hsap\DAAM1
2 of 15
No
No
Hsap\DAAM2
2 of 15
No
No
Hsap\GRID2IP
2 of 15
No
No
Hsap\INF2
2 of 15
No
No
Hsap\FHDC1
1 of 15
No
No
Hsap\FMN1
1 of 15
No
No
Hsap\FMN2
1 of 15
No
No
Hsap\FMNL1
1 of 15
No
No
Hsap\FMNL2
1 of 15
No
No
Hsap\FMNL3
1 of 15
No
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (13)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Diaph1
14 of 15
Yes
Yes
Mmus\Diaph3
13 of 15
No
Yes
Mmus\Diaph2
11 of 15
No
Yes
Mmus\Daam1
2 of 15
No
No
 
Mmus\Daam2
2 of 15
No
No
Mmus\Inf2
2 of 15
No
No
Mmus\Fhdc1
1 of 15
No
No
Mmus\Fmn1
1 of 15
No
No
Mmus\Fmn2
1 of 15
No
No
Mmus\Fmnl1
1 of 15
No
No
Mmus\Fmnl2
1 of 15
No
No
Mmus\Fmnl3
1 of 15
No
No
Mmus\Grid2ip
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
Rnor\Diaph3
8 of 13
Yes
Yes
Rnor\Diaph1
6 of 13
No
Yes
Rnor\Daam1
1 of 13
No
No
Rnor\Daam2
1 of 13
No
No
Rnor\Diaph2
1 of 13
No
Yes
Rnor\Fhdc1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (5)
Xtro\diaph2
4 of 12
Yes
Yes
Xtro\diaph1
2 of 12
No
Yes
Xtro\inf2
2 of 12
No
No
Xtro\diaph3
1 of 12
No
Yes
Xtro\fmnl1
1 of 12
No
No
Danio rerio (Zebrafish) (11)
Drer\diaph2
6 of 15
Yes
Yes
Drer\diaph3
4 of 15
No
Yes
Drer\daam1b
2 of 15
No
No
Drer\fhdc4
2 of 15
No
No
Drer\fhdc1
1 of 15
No
No
Drer\fhdc2
1 of 15
No
No
Drer\fmn1
1 of 15
No
No
Drer\fmn2b
1 of 15
No
No
Drer\fmnl2a
1 of 15
No
No
Drer\grid2ipb
1 of 15
No
No
Drer\inf2
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\cyk-1
10 of 15
Yes
Yes
Cele\daam-1
3 of 15
No
No
Cele\exc-6
1 of 15
No
No
Cele\F59B1.4
1 of 15
No
Yes
Cele\fbxc-26
1 of 15
No
Yes
Cele\frl-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (15)
Atha\AFH14
2 of 9
Yes
No
Atha\AT3G07540
2 of 9
Yes
Yes
Atha\AT3G32400
2 of 9
Yes
No
Atha\AT5G48360
2 of 9
Yes
Yes
Atha\AFH1
1 of 9
No
Yes
Atha\AT1G42980
1 of 9
No
Yes
Atha\AT1G59910
1 of 9
No
Yes
Atha\AT2G43800
1 of 9
No
Yes
Atha\AT3G05470
1 of 9
No
Yes
Atha\AT5G58160
1 of 9
No
No
Atha\FH4
1 of 9
No
Yes
Atha\Fh5
1 of 9
No
Yes
Atha\FH6
1 of 9
No
Yes
Atha\FH8
1 of 9
No
Yes
Atha\FH19
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\BNI1
2 of 15
Yes
No
Scer\BNR1
2 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\for3
5 of 12
Yes
Yes
Spom\cdc12
3 of 12
No
Yes
Spom\fus1
3 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902DM )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\dia
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000815
Drosophila simulans
Dsim\GD24279
Drosophila sechellia
Dsec\GM23367
Drosophila erecta
Dere\GG21250
Drosophila yakuba
Dyak\GE12345
Drosophila ananassae
Dana\GF15354
Drosophila pseudoobscura pseudoobscura
Dpse\GA14608
Drosophila persimilis
Dper\GL18518
Drosophila willistoni
Dwil\GK14913
Drosophila virilis
Dvir\GJ17693
Drosophila mojavensis
Dmoj\GI17921
Drosophila grimshawi
Dgri\GH11618
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915016I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013564
Musca domestica
House fly
Mdoa\17013565
Glossina morsitans
Tsetse fly
Gmor\GMOY003530
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08891
Mayetiola destructor
Hessian fly
Mdes\Mdes010396
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012283
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL014868
Anopheles darlingi
American malaria mosquito
Adar\ADAC007729
Anopheles gambiae
Malaria mosquito
Agam\AGAP009643
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ000980
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00ZH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA011175
Danaus plexippus
Monarch butterfly
Dple\DPOGS203185-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS203186-PA
Apis florea
Little honeybee
Aflo\2256740836
Apis mellifera
Western honey bee
Amel\GB55272
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22165
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22164
Bombus terrestris
Buff-tailed bumblebee
Bter\15212157
Linepithema humile
Argentine ant
Lhum\LH10922
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:003183
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG012917
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03169
Tribolium castaneum
Red flour beetle
Tcas\TC006029
Pediculus humanus
Human body louse
Phum\PHUM368970
Rhodnius prolixus
Kissing bug
Rpro\RPRC013727
Cimex lectularius
Bed bug
Clec\CLEC012377
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI002122
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10929
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00XQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR010881
Ixodes scapularis
Black-legged tick
Isca\ISCW017338
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59583.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59584.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59582.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur19g00980
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_220427
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04ZJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Diaph_1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
DAAM
4 of 10
form3
3 of 10
Frl
3 of 10
capu
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
diaUASp.EGFP
ameliorates  cancer
modeled by MycαTub84B.PBb
(Levayer et al., 2015)
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
DIAPH3; diaphanous related formin 3
13 of 15
614567
DIAPH1; diaphanous related formin 1
12 of 15
602121
DIAPH2; diaphanous related formin 2
12 of 15
300108
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement
View in Interactions Browser
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
dia
enhanceable
Apc2
(Webb et al., 2009)
dia
suppressible
ex
(Sansores-Garcia et al., 2011)
dia
suppressible
hpo
(Sansores-Garcia et al., 2011)
dia
suppressible
spi
(Peng et al., 2012)
dia
suppressible
wts
(Sansores-Garcia et al., 2011)
dia
suppressible
yki
(Sansores-Garcia et al., 2011)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Abl
enhanceable
dia
(Grevengoed et al., 2003)
Apc2
enhanceable
dia
(Webb et al., 2009)
Csk
enhanceable
dia
(Rudrapatna et al., 2014)
CycB
suppressible
dia
(Ji et al., 2002)
CycB
enhanceable
dia
(Ji et al., 2002)
Egfr|psq
suppressible
dia
(Fereres et al., 2019)
LIMK1
suppressible
dia
(Chen et al., 2004, Ng and Luo, 2004)
Myc
suppressible
dia
(Levayer et al., 2015)
pbl
enhanceable
dia
(Gregory et al., 2007, Prokopenko et al., 1999)
pbl
suppressible
dia
(Gregory et al., 2007)
Ret
suppressible
dia
(Fereres et al., 2019)
Rho1
suppressible
dia
(Prokopenko et al., 1999)
Rho1
enhanceable
dia
(Homem and Peifer, 2008)
Rok
suppressible
dia
(Verdier et al., 2006)
Rok
enhanceable
dia
(Warner and Longmore, 2009)
shot
suppressible
dia
(Sun et al., 2019)
step
suppressible
dia|zip
(Lee and Harris, 2013)
yki
enhanceable
dia
(Gerlach et al., 2018)
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map

2-54

Cytogenetic map
38E7-38E8
Sequence location
2L:20,758,144..20,768,053 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
38E7-38E8
Limits computationally determined from genome sequence between P{PZ}Hr3802306&P{PZ}dia1 and P{EP}CG31673EP432
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
38A1-39E1
(Giansanti et al., 2004)
38E5-38E6
(determined by in situ hybridisation)
(Spradling et al., 1999)
38E1-38E10
38E5--6
(BDGP Project Members, 1994-1999)
Location based on deficiency mapping.
(Giansanti et al., 2004)
Experimentally Determined Recombination Data
Location

2-54

(FlyBase, 2016)

2-54.1

(Comeron et al., 2012)

2-

(Giansanti et al., 2004)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (22)
Genomic Clones (21)
cDNA Clones (40)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: dia CG1768

(Lei, 1999.11)
Source for database merge of

Source for merge of: dia l(2)k07135

(Spradling et al., 1999)
Additional comments

null alleles lethal.

Other Comments

dia is required for establishing and maintaining distinct lateral and basal domains during cellularisation. Mutant embryos show an increased number of membrane extensions and endocytic activity in the basal domain, indicating a suppressing role of dia on membrane invaginations.

(Yan et al., 2013)

dia is required for generation of apical F-actin in diverse types of epithelial tubes in the embryo.

(Massarwa et al., 2009)

dia is required for furrow canal formation during cellularisation.

(Großhans et al., 2005)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.

(Eggert et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

dia has a role in actin cytoskeleton organisation.

(Afshar et al., 2000)

dia function is required for cytokinesis.

(Castrillon and Wasserman, 1994)

Proliferation defect locus.

(Castrillon et al., 1993)
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 68 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
InterPro - A database of protein families, domains and functional sites
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DPiM - Drosophila Protein interaction map
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
38E.16
(Butler, 2001, Butler et al., 2001, McGill Drosophila Genome Project, 2001.9.25)
CG1768
(Çiçek et al., 2016, Hosono et al., 2015, Christis and Munro, 2012, Howell et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Rogers et al., 2003, Goldstein and Gunawardena, 2000)
DIA
(D'Avino et al., 2005)
Dia
(Adler, 2018, Schmidt and Grosshans, 2018, Chung et al., 2017, Gilmour et al., 2017, Tran and Ten Hagen, 2017, Bothe and Baylies, 2016, Enomoto et al., 2015, Flores-Benitez and Knust, 2015, Levayer et al., 2015, Rodal et al., 2015, Rosa et al., 2015, Abreu-Blanco et al., 2014, Acharya et al., 2014, Haralalka et al., 2014, Lammel et al., 2014, Li et al., 2014, Nowotarski et al., 2014, Pichaud, 2014, Poukkula et al., 2014, Antunes et al., 2013, Jaiswal et al., 2013, Khoo et al., 2013, Lee and Harris, 2013, Matsui and Lai, 2013, Parkhurst, 2013.10.2, Zhang et al., 2013, Förster and Luschnig, 2012, Rojas-Ríos et al., 2012, Laplante and Nilson, 2011, Muyskens and Guillemin, 2011, Blanchard et al., 2010, Laplante et al., 2010, Warner and Longmore, 2009, Warner and Longmore, 2009, Cavey et al., 2008, Hickson and O'Farrell, 2008, Homem and Peifer, 2008, Llense and Martín-Blanco, 2008, Mulinari et al., 2008, Stevens et al., 2008, Bakal et al., 2007, Homem and Peifer, 2007, Webb et al., 2007, Matusek et al., 2006, Price et al., 2006, Padash Barmchi et al., 2005, Zavortink et al., 2005, Pedra et al., 2004)
Dias
(Bertet et al., 2009)
dia
(Blake-Hedges and Megraw, 2019, Boucher et al., 2019, Gao et al., 2019, Jiang et al., 2019, Meltzer et al., 2019, Myat et al., 2019, Tegeder et al., 2019, Córdoba and Estella, 2018, Du et al., 2018, Vu et al., 2018, Cheng et al., 2017, Couturier et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Huang et al., 2016, Rousso et al., 2016, Aradska et al., 2015, Davis et al., 2015, Goins and Mullins, 2015, Munjal et al., 2015, Ashwal-Fluss et al., 2014, Pézeron et al., 2014, Roy et al., 2014, Rudrapatna et al., 2014, Founounou et al., 2013, Külshammer and Uhlirova, 2013, Lee and Harris, 2013, Rousso et al., 2013, Vaque et al., 2013, Yan et al., 2013, Giansanti et al., 2012, Howell et al., 2012, Lesch and Page, 2012, Peng et al., 2012, Friedman et al., 2011, Guan et al., 2011, Levayer et al., 2011, Neumüller et al., 2011, Tucker et al., 2011, Vlachos and Harden, 2011, Cao et al., 2010, Kadandale et al., 2010, Neisch et al., 2010, Wasbrough et al., 2010, Wasbrough et al., 2010, Bertet et al., 2009, Delon and Brown, 2009, Homem and Peifer, 2009, Massarwa et al., 2009, Webb et al., 2009, Widmann and Dahmann, 2009, Castellanos et al., 2008, Cosetti et al., 2008, Homem and Peifer, 2008, Matusek et al., 2008, Mulinari et al., 2008, Pawson et al., 2008, Bakal et al., 2007, Corrigall et al., 2007, Pichaud et al., 2007, Sandmann et al., 2007, Stuart et al., 2007, Wang and Riechmann, 2007, Williams et al., 2007, Hickson et al., 2006, Janody and Treisman, 2006, Kuranaga et al., 2006, Verdier et al., 2006, Padash Barmchi et al., 2005)
l(2)k07135
(BDGP Project Members, 1994-1999)
ms(2)04138
Name Synonyms
Diaphanous
(Kobb et al., 2019, Adler, 2018, Córdoba and Estella, 2018, Tran and Ten Hagen, 2017, Martin, 2016, Rousso et al., 2016, Goins and Mullins, 2015, Levayer et al., 2015, Rikhy et al., 2015, Rosa et al., 2015, Abreu-Blanco et al., 2014, Gupta et al., 2014, Li et al., 2014, Nowotarski et al., 2014, Antunes et al., 2013, Khoo et al., 2013, Lee and Harris, 2013, Rousso et al., 2013, Gates, 2012, Laplante and Nilson, 2011, Blanchard et al., 2010, Castoreno et al., 2010, Neisch et al., 2010, Delon and Brown, 2009, Homem and Peifer, 2009, Webb et al., 2009, Cao et al., 2008, Cavey et al., 2008, Homem and Peifer, 2008, Homem and Peifer, 2008, Mulinari et al., 2008, Prokopenko and Chia, 2008, Homem and Peifer, 2007, Prokopenko and Chia, 2007, Webb et al., 2007, Price et al., 2006, Higgs and Peterson, 2005, Zavortink et al., 2005, Rogers et al., 2003)
diaphanous
(Roy et al., 2014, Rudrapatna et al., 2014, Giansanti et al., 2012, Lesch and Page, 2012, Guan et al., 2011, Cao et al., 2010, Schoen et al., 2010, Wasbrough et al., 2010, Shalaby et al., 2009, Castellanos et al., 2008, Cosetti et al., 2008, Hoopfer et al., 2008, Pawson et al., 2008, Silverman-Gavrila et al., 2008, Gregory et al., 2007, Pichaud, 2007, Pichaud et al., 2007, Wang and Riechmann, 2007, Williams et al., 2007, Minin et al., 2006, Verdier et al., 2006, Dean et al., 2005, Padash Barmchi et al., 2005, Lecuit, 2004)
Secondary FlyBase IDs
  • FBgn0022100
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (281)