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Gene Dmel\dia

General Information
Symbol	Dmel\dia	Species	D. melanogaster
Name	diaphanous	Annotation symbol	CG1768
Feature type	protein_coding_gene	FlyBase ID	FBgn0011202
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	38E.16
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	38E7-38E8	Sequence location	2L:20,758,144..20,768,053 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene diaphanous is referred to in FlyBase by the symbol Dmel\dia (CG1768, FBgn0011202). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence for 21 unique biological process terms, many of which group under: cell cycle; cellular component organization or biogenesis; single-organism developmental process; biological regulation; localization; cell cycle process; system development; multicellular organism reproduction; actin filament-based process; multicellular organismal development. 31 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; portion of tissue; organ system subdivision; anatomical structure; cell part; non-connected developing system; epithelial cell; cell projection; cytoplasmic part; imaginal precursor; external compound sense organ; late extended germ band embryo. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Actin-binding FH2; Actin-binding FH2/DRF autoregulatory; Armadillo-type fold; DRF autoregulatory; Diaphanous FH3; Diaphanous GTPase-binding; Diaphanous autoregulatory; GTPase-binding/formin homology 3. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, larval/adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, larval/adult midgut, larval hindgut, larval/adult salivary gland, adult female reproductive system, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1625942_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of dia. Gene sequence location is 2L:20758144..20768053.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38E7-38E8   Limits computationally determined from genome sequence between P{PZ}Hr3802306&P{PZ}dia1 and P{EP}CG31673EP432
Experimentally Determined Cytological Location
Cytogenetic map Notes References 38E5-38E6   (determined by in situ hybridisation)   (Spradling et al., 1999) 38E1-38E10   38E5--6   (BDGP Project Members, 1994-1999) 38A1-39E1   Location based on deficiency mapping.   (Giansanti et al., 2004)
Experimentally Determined Recombination Data
Location	2- (Giansanti et al., 2004)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\dia for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) dia-RA FBtr0081410  NM_057633   4743   1091 dia-RB FBtr0081411  NM_165341   4465   1091 dia-RC FBtr0305960  NM_001259183   1775   130 dia-RD FBtr0305961  NM_001259184   3797   1098 dia-RE FBtr0335145  NM_001273711   5260   1091
Additional Transcript Data & Comments
Reported size (kB)	4.8, 4.4 (northern blot) (Castrillon and Wasserman, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein dia-PA   FBpp0080940   123.2   1091   8.25     NP_476981   AAF53922 dia-PB   FBpp0080941   123.2   1091   8.25     NP_724285   AAN11087 dia-PC   FBpp0297102   14.5   130   10.05     NP_001246112   AFH03786 dia-PD   FBpp0297103   124.0   1098   8.51     NP_001246113   AFH03787 dia-PE   FBpp0307144   123.2   1091   8.25     NP_001260640   AGB93175
Additional Polypeptide Data & Comments
Reported size (kDa)	1091 (aa); 123 (kD predicted) (Castrillon and Wasserman, 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Actin-binding FH2/DRF autoregulatory (IPR003104) DRF autoregulatory (IPR010465) Diaphanous FH3 (IPR010472) Diaphanous autoregulatory (IPR014767) GTPase-binding/formin homology 3 (IPR014768) Actin-binding FH2 (IPR015425) Diaphanous GTPase-binding (IPR010473) Armadillo-type fold (IPR016024)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142035   AA392355   AA439629   AA441512   AA536376   AA802096   AE014134 AAF53922   AE014134 AAN11087   AC004759   AC007467   AE014134 AFH03786   AE014134 AFH03787   AE014134 AGB93175   AI257364   AI293781   AI515699   AI946333   AQ025819   AQ026407   BG636029   BG641469   BI216639   BI239230   BI638524   BI638842   BT021398 AAX33546   BT120071   BT120072   BT120147   CK657499   CL844426   CO264241   CO268943   CO275614   CO289588   CO290616   CO293622   DN154231   EC061518   EC063745   EC068732   EC068821   EC070384   EC074922   EC082535   EC086747   EC197186   EC199931   EC207707   EC225351   EC231940   L80120   U11288 AAA67715
UniProtKB/Swiss-Prot	P48608
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage | female     (Castrillon and Wasserman, 1994) adult stage | male     (Castrillon and Wasserman, 1994)
Additional Descriptive Data
	dia transcripts are expressed in both males and females and are not germline specific. (Castrillon and Wasserman, 1994)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with actin filament inferred from direct assay (Webb et al., 2009) adherens junction inferred from direct assay (Homem and Peifer, 2008) apical part of cell inferred from direct assay (Massarwa et al., 2009, Corrigall et al., 2007) cell cortex inferred from direct assay (Homem and Peifer, 2008) cleavage furrow inferred from direct assay (Homem and Peifer, 2008) protein complex inferred from physical interaction with Apc2 (Webb et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, larval/adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, larval/adult midgut, larval hindgut, larval/adult salivary gland, adult female reproductive system, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules  513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop  531.6 Adult Midgut    431.4 Adult Hindgut  (783.1) Adult Malpighian Tubules  (640.2) Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules  513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop  531.6 Adult Midgut    431.4 Adult Hindgut  783.1 Adult Malpighian Tubules  640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    164.7   NA  Eye    39.425   NA  Brain    150.4   28.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    137.2   NA  Crop    531.6   148.1  Midgut    431.4   189.1  Hindgut    783.1   513.2  Malpighian Tubules    640.2   68.6  Fat Body    38.2   140.1  Salivary Gland    102.7   NA  Heart    43.3   76.6  Trachea    NA   NA  VirginFemale Spermatheca    75.2   NA  InseminatedFemale Spermatheca    117.6   NA  Ovary    263.2   NA  Testis    45.4   NA  Male Accessory Gland    126.9   77.85  Carcass    81.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering  (39) digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering  (39) digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    8 central nervous system, larvae L3    6 central nervous system, pupae P8    4 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    12 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    17 digestive system, 4-day adult    19 digestive system, 20-day adult    18 fat body, larvae L3 wandering    4 fat body, white prepupae    12 fat body, pupae P8    9 carcass, larvae L3 wandering    8 carcass, 1-day adult    6 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    16 ovary, mated 4-day female    13 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages, during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    13 embryo 04-06hr    16 embryo 06-08hr    14 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    10 embryo 14-16hr    10 embryo 16-18hr    12 embryo 18-20hr    16 embryo 20-22hr    15 embryo 22-24hr    18 larva L1    7 larva L2    6 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    15 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    7 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2    44 embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    42 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    34 ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2  (44) embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3  (42) wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2  (34) ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2    44 embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    42 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    34 ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2    44 embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    42 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    34 ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2    44 embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    42 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    34 ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2  (44) embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3  (42) wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2  (34) ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2    44 embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    42 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    34 ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    16 embryonic 1182-4H    13 embryonic GM2    44 embryonic Kc167    15 embryonic S1    17 embryonic S3    10 leg disc CME L1    23 wing disc CME-W2    8 wing disc ML-DmD8    8 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    42 wing disc ML-DmD21    9 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    34 ovary fGS/OSS    7 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    8 heat shock, 4-day adult    10 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    12 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011202 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1768-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal diaGD9442 diaKK101745 lethal | embryonic stage, with Scer\GAL4e22c diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP diaFH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP lethal | embryonic stage | non-rescuable maternal effect | recessive dia5 lethal | maternal effect | recessive dia9 lethal | pupal stage, with Scer\GAL4ph-p-NP0761 diaCA.Scer\UAS.T:Ivir\HA1 lethal | pupal stage | recessive dia2 dia3 lethal | recessive dia3 dia5 dia9 dia38 dia40 dia50 dia55 diak07135 diak07140 partially lethal - majority die, with Scer\GAL4ph-p-NP1046 diaCA.Scer\UAS.T:Ivir\HA1 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 diaGD9442 viable diaEY11231 Sterility Allele female sterile | recessive dia1 fertile diaEY11231 male sterile dia1 male sterile | recessive dia1 Other Phenotypes Allele auditory perception defective, with Scer\GAL4CG13795-NP1245 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective, with Scer\GAL4JO15 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective, with Scer\GAL4NP6303 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective, with Scer\GAL4ph-p-NP1046 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective | dominant dia5 cell shape defective | extended germ band stage diaCA.Scer\UAS.T:Ivir\HA1 cytokinesis defective | male (with Df(2L)TW84) diaZ5930 cytokinesis defective | male | recessive diaZ4053 diaZ5930 cytokinesis defective | recessive dia1 dia5 cytokinesis defective | somatic clone dia5 immune response defective | larval stage, with Scer\GAL4Hml.PG diaKK101745 meiotic cell cycle defective | male (with Df(2L)TW84) diaZ5930 meiotic cell cycle defective | male | recessive diaZ4053 diaZ5930 mitotic cell cycle defective diadsRNA.cHa mitotic cell cycle defective | male (with Df(2L)TW84) diaZ5930 mitotic cell cycle defective | male | recessive diaZ4053 neuroanatomy defective, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 neuroanatomy defective | larval stage diaGD9442 neurophysiology defective | dominant dia5 visible, with Scer\GAL4pnr-MD237 diaGD9442 wild-type dia+t11 Phenotype manifest in Allele actin filament & embryonic/larval tracheal system dia5 actin filament & embryonic/larval tracheal system, with Scer\GAL4btl.PS diaNIG.1768R diaGD9442 actin filament | embryonic stage | germline clone dia5 amnioserosa, with Scer\GAL4c381 diaCA.Scer\UAS.T:Ivir\HA1 amnioserosa, with Scer\GAL4en-e16E diaCA.Scer\UAS.T:Ivir\HA1 amnioserosa | maternal effect (with dia2) dia5 amnioserosa | maternal effect (with dia5) dia2 blastoderm embryo | germline clone dia5 border follicle cell, with Scer\GAL4slbo.2.6 diaCA.Scer\UAS.T:Ivir\HA1 bract | somatic clone dia5 centrosome | embryonic stage | germline clone dia5 commissure, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 connective, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 contractile ring & meiotic cell cycle | male dia1 dia3 diaZ5930 contractile ring & meiotic cell cycle | male (with Df(2L)TW84) diaZ5930 cortical actin cytoskeleton & syncytial blastoderm embryo | maternal effect dia5 diak07135 cycle 11 embryo | germline clone dia5 cyst cell dia1 cytoskeleton | embryonic stage | germline clone dia5 denticle belt | germline clone dia5 dorsal closure embryo | germline clone dia5 ectoderm | extended germ band stage diaCA.Scer\UAS.T:Ivir\HA1 egg chamber dia1 egg chamber | somatic clone dia5 embryonic/first instar larval cuticle | germline clone dia5 embryonic/larval dorsal trunk, with Scer\GAL4btl.PS diaCA.Scer\UAS.T:Ivir\HA1 embryonic/larval dorsal trunk, with Scer\GAL4en-e16E diaCA.Scer\UAS.T:Ivir\HA1 embryonic/larval dorsal trunk, with Scer\GAL4prd.RG1 diaCA.Scer\UAS.T:Ivir\HA1 embryonic/larval neuroblast | larval stage diaGD9442 embryonic epidermis, with Scer\GAL4en-e16E diaCA.Scer\UAS.T:Ivir\HA1 embryonic epidermis, with Scer\GAL4prd.RG1 diaCA.Scer\UAS.T:Ivir\HA1 embryonic head | germline clone dia5 embryonic leading edge cell, with Scer\GAL4en-e16E diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP epidermal cell, with Scer\GAL4en-e16E diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP epithelial cell | somatic clone, with Scer\GAL4Act5C.PI diaCA.Scer\UAS.T:Ivir\HA1 epithelium | somatic clone dia5 eye dia9 filopodium dia5 filopodium, with Scer\GAL4en-e16E diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP filopodium | ectopic, with Scer\GAL4en-e16E diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP follicle cell dia1 follicle cell | somatic clone dia5 furrow canal | germline clone dia5 ganglion mother cell | larval stage diaGD9442 gastrula embryo | germline clone dia5 imaginal disc dia2 dia3 lamellipodium dia5 lamellipodium, with Scer\GAL4en-e16E diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP larval central nervous system & neuroblast dia2 dia3 dia9 mesothoracic tergum, with Scer\GAL4pnr-MD237 diaGD9442 microtubule | embryonic stage | germline clone dia5 morphogenetic furrow | somatic clone dia5 Nebenkern dia1 diaZ4053 diaZ5930 Nebenkern (with Df(2L)TW84) diaZ5930 nucleus | embryonic stage | germline clone dia5 organism | dorsal closure stage, with Scer\GAL4c381 diaCA.Scer\UAS.T:Ivir\HA1 organism | dorsal closure stage | maternal effect (with dia2) dia5 organism | dorsal closure stage | maternal effect (with dia5) dia2 pole bud | germline clone dia5 pole cell | germline clone dia5 presumptive embryonic/larval central nervous system, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 S2 cell-line & spindle microtubule diadsRNA.cHa spermatid dia1 spermatid & nucleus | supernumerary diaZ4053 diaZ5930 spermatid & nucleus | supernumerary (with Df(2L)TW84) diaZ5930 spermatocyte dia1 spindle & meiotic cell cycle | male dia3 diaZ5930 spindle & meiotic cell cycle | male (with Df(2L)TW84) diaZ5930 testis dia1 tormogen cell | somatic clone dia5 tracheal fusion cell, with Scer\GAL4btl.PS diaCA.Scer\UAS.T:Ivir\HA1 ventral furrow | non-rescuable maternal effect dia5
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of dia Class Mutagen Stocks Known lesion diak07135 P-element activity 3 -- dia1 hypomorphic allele - genetic evidence P-element activity 2 Yes dia5 loss of function allele, hypomorphic allele - genetic evidence Delta2-3 1 Yes diaGS2135 1 Yes diak07140 P-element activity 1 -- dia2 amorphic allele - genetic evidence Delta2-3 0 Yes dia38 ethyl methanesulfonate 0 -- dia3 hypomorphic allele - genetic evidence Delta2-3 0 Yes dia40 ethyl methanesulfonate 0 -- dia50 ethyl methanesulfonate 0 -- dia55 ethyl methanesulfonate 0 -- dia9 hypomorphic allele - genetic evidence Delta2-3 0 Yes diaEY11231 P-element activity 0 -- diaunspecified 0 -- diaZ4053 ethyl methanesulfonate 0 -- diaZ5930 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of dia Class Mutagen Stocks Known lesion diaCA.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion 1 Yes diaGD9442 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaGL00408 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaHM05027 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaHMS00308 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaKK101745 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes dia+t11 in vitro construct - genomic fragment 0 Yes diaDDFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - coding region fusion 0 Yes diadsRNA.cHa in vitro construct - RNAi 0 -- diadsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - coding region fusion 0 Yes diaFH3FH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - coding region fusion 0 Yes diaNIG.1768R in vitro construct - RNAi 0 -- diaScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes diaΔDad.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)DS5 (Butler et al., 2001) Df(2L)DS6 (Kozlova et al., 1998) Df(2L)DS9 (Kozlova et al., 1998, Butler et al., 2001) Df(2L)Fs(2)Ket-RX32 (Kozlova et al., 1998, Butler et al., 2001) Df(2L)pr1122 (Butler et al., 2001) Df(2L)pr9204 (Butler et al., 2001) Df(2L)pr11163 (Butler et al., 2001) Df(2L)TW2 (Butler et al., 2001) Df(2L)TW65 (Butler et al., 2001) Df(2L)TW84 (Butler et al., 2001, Giansanti et al., 2004, Castrillon et al., 1993, BDGP Project Members, 1994-1999, Castrillon and Wasserman, 1994) Df(2L)TW161 (BDGP Project Members, 1994-1999) Df(2L)VA6 (Butler et al., 2001, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2L)Acon-21 (Butler et al., 2001) Df(2L)DS8 (Butler et al., 2001) Df(2L)pr1 (Butler et al., 2001) Df(2L)pr2b (Butler et al., 2001) Df(2L)pr37 (Butler et al., 2001) Df(2L)pr9201 (Butler et al., 2001) Df(2L)pr-A16 (Butler et al., 2001) Df(2L)TW50 (Butler et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9442} NA P{KK101745} NA P{TRiP.GL00408} NA P{TRiP.HM05027} NA P{TRiP.HMS00308} NA P{UAS-dia.CA} NA P{UAS-dia.RNAi} NA P{UASp-dia.DDFH1FH2.EGFP} NA P{UASp-dia.EGFP} NA P{UASp-dia.FH1FH2.EGFP} NA P{UASp-dia.FH3FH1FH2.EGFP} NA P{UASp-dia.ΔDad.EGFP} NA characterization construct P{DC4} NA P{NIG.1768R} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}diak07135 No P{lacW}diak07140 No P{lacW}l(2)SH0694SH0694 No P{PZ}dia1 No insertion of mobile activating element P{EPgy2}diaEY11231 NA P{GSV1}diaGS2135 NA miscellaneous insertions P{?PZ}dia5 NA
Gene Ontology: Function, Process & Cellular Component ( 32 unique terms )
Terms Based on Experimental Evidence ( 28 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Apc2 (Webb et al., 2009) inferred from physical interaction with chic (Webb et al., 2009) inferred from physical interaction with Eb1 (Webb et al., 2009)
Biological Process
CV term Evidence References
actin filament organization inferred from mutant phenotype (Afshar et al., 2000, Kiger et al., 2003, Grosshans et al., 2005) apical constriction inferred from mutant phenotype (Bertet et al., 2009) cellularization inferred from mutant phenotype (Afshar et al., 2000, Grosshans et al., 2005) cytokinesis inferred from mutant phenotype (Kiger et al., 2003, Rogers et al., 2003, Echard et al., 2004, Eggert et al., 2004, Dean et al., 2005) cytokinesis, actomyosin contractile ring assembly inferred from mutant phenotype (Giansanti et al., 2004) cytokinesis after meiosis I inferred from mutant phenotype (Giansanti et al., 2004) cytokinesis after meiosis II inferred from mutant phenotype (Giansanti et al., 2004) maintenance of protein location in cell inferred from mutant phenotype (Dean et al., 2005) melanotic encapsulation of foreign target inferred from mutant phenotype (Howell et al., 2012) mitotic cell cycle, embryonic inferred from mutant phenotype (Afshar et al., 2000) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) open tracheal system development inferred from mutant phenotype (Matusek et al., 2006) pole cell formation inferred from mutant phenotype (Afshar et al., 2000) protein localization inferred from mutant phenotype (Afshar et al., 2000) protein secretion inferred from mutant phenotype (Massarwa et al., 2009) pseudocleavage involved in syncytial blastoderm formation inferred from mutant phenotype (Webb et al., 2009) regulation of embryonic cell shape inferred from mutant phenotype (Homem and Peifer, 2008, Bertet et al., 2009) regulation of filopodium assembly inferred from mutant phenotype (Homem and Peifer, 2009) sensory perception of sound inferred from mutant phenotype (Cosetti et al., 2008, Schoen et al., 2010) spermatogenesis inferred from mutant phenotype (Castrillon et al., 1993) spindle assembly inferred from mutant phenotype (Giansanti et al., 2004)
Cellular Component
CV term Evidence References
colocalizes_with actin filament inferred from direct assay (Webb et al., 2009) adherens junction inferred from direct assay (Homem and Peifer, 2008) apical part of cell inferred from direct assay (Massarwa et al., 2009, Corrigall et al., 2007) cell cortex inferred from direct assay (Homem and Peifer, 2008) cleavage furrow inferred from direct assay (Homem and Peifer, 2008) protein complex inferred from physical interaction with Apc2 (Webb et al., 2009)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
actin binding inferred from electronic annotation with InterPro:IPR003104, InterPro:IPR010472, InterPro:IPR010473 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) Rho GTPase binding inferred from electronic annotation with InterPro:IPR010473 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
actin filament-based process traceable author statement (Van Aelst and Symons, 2002, Mazumdar and Mazumdar, 2002) cellularization traceable author statement (Tepass et al., 2001, Mazumdar and Mazumdar, 2002) cytokinesis non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) traceable author statement (Van Aelst and Symons, 2002, Mazumdar and Mazumdar, 2002) cytokinesis, actomyosin contractile ring assembly non-traceable author statement (Tepass et al., 2001) protein localization non-traceable author statement (Gonzalez and Bornens, 2000) traceable author statement (Mazumdar and Mazumdar, 2002)
Cellular Component
CV term Evidence References
actomyosin contractile ring non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
dia - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - Int6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - Plod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) dia - eIF3-S8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions dia allele Gene References dia1 CycB (Ji et al., 2002) LIMK1 (Chen et al., 2004) dia2 CycB (Ji et al., 2002) pbl (Prokopenko et al., 1999) Rho1 (Prokopenko et al., 1999) dia5 LIMK1 (Ng and Luo, 2004) dia9 CycB (Ji et al., 2002) diak07135 LIMK1 (Chen et al., 2004) unspecified pbl (Gregory et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61303 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011202 DroID - A comprehensive database of gene and protein interactions. • FBgn0011202 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35340
Orthologs
OrthoDB Orthologs (34) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG60P4S5 Dipteran inclusive ortholog search EOG66T38H Insect inclusive ortholog search EOG605QVZ Arthropod inclusive ortholog search EOG6H189M Metazoa inclusive ortholog search EOG6ZD5X8
Orthologs in Drosophila Species (EOG60P4S5)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\dia     Drosophila simulans   Dsim\GD24279 Y   Drosophila sechellia   Dsec\GM23367 Y   Drosophila erecta   Dere\GG21250 Y   Drosophila yakuba   Dyak\GE12345 Y   Drosophila ananassae   Dana\GF15354 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14608 Y   Drosophila persimilis   Dper\GL18518 Y   Drosophila willistoni   Dwil\GK14913 Y   Drosophila virilis   Dvir\GJ17693 Y   Drosophila mojavensis   Dmoj\GI17921 Y   Drosophila grimshawi   Dgri\GH11618 Y
Orthologs in non-Drosophila Dipterans (EOG66T38H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012283   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014868   Anopheles gambiae Malaria mosquito  Agam\AGAP009643   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000980
Orthologs in non-Dipteran Insects (EOG605QVZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB55272   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012917   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH25862   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH27253   Atta cephalotes Leafcutter ant  Acep\ACEP13975   Atta cephalotes Leafcutter ant  Acep\ACEP13976   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17992   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14614   Linepithema humile Argentine ant  Lhum\LH10922   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21742   Solenopsis invicta Red fire ant  Sinv\SINV15397   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002122   Bombyx mori Silkmoth  Bmor\BGIBMGA011175   Pediculus humanus Human body louse  Phum\PHUM368970   Tribolium castaneum Red flour beetle  Tcas\TC006029
Orthologs in non-Insect Arthropods (EOG6H189M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\ndhF
Orthologs in non-Arthropod Metazoa (EOG6ZD5X8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F11H8.4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012710gn
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24279 Drosophila sechellia Dsec\GM23367 Drosophila erecta Dere\GG21250 Drosophila yakuba Dyak\GE12345 Drosophila ananassae Dana\GF15354 Drosophila pseudoobscura pseudoobscura Dpse\GA14608 Drosophila persimilis Dper\GL18518 Drosophila willistoni Dwil\GK14913 Drosophila virilis Dvir\GJ17693 Drosophila mojavensis Dmoj\GI17921 Drosophila grimshawi Dgri\GH11618
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	27616 w*; P{UAS-dia.CA}3 9138 w*; dia5 P{neoFRT}40A/CyO 28541 y1 v1; P{TRiP.HM05027}attP2 33424 y1 sc* v1; P{TRiP.HMS00308}attP2 35479 y1 sc* v1; P{TRiP.GL00408}attP40/CyO
Kyoto	108878 P{PZ}dia1 cn1/CyO; ry506 108859 y1 w67c23; P{lacW}diak07135/CyO 205048 y1 w67c23; P{GSV1}diaGS2135/SM1 102532 y1 w67c23 ; P{lacW}diak07135 / CyO 102535 y1 w67c23 ; P{lacW}diak07140 / CyO
VDRC	v103914 P{KK101745}VIE-260B v20518 w1118; P{GD9442}v20518
	More stocks available...
Genomic Clones ( 1 )
	BACR07D17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD14246
Other clones	DPiM_LD14246 MIP15714 MIP15814 MIP15914
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM03848 LD11226 LD16477 LD16963 LD41344 LP05703 LP06996 LP23848 RE23844 RE35666 SD12820 SD13607 SD20829 SD21236
Other clones	34943 53725 69522 73490 74408 103116 bs23g02 CK01.20D.C7 CK01.22A.F6 CK01.91B.C5 CK01.95D.D8 CK01.121D.C3 CK01.121D.C12 CK00152 EK071411 EK074534 EK093142 EK118048 EK138303 EK202451 EK203851
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011202 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35340
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: dia CG1768 (Lei, 1999.11)
Source for database merge of	Source for merge of: dia l(2)k07135 (Spradling et al., 1999)
Additional comments	null alleles lethal.
Other Comments
	dia is required for generation of apical F-actin in diverse types of epithelial tubes in the embryo. (Massarwa et al., 2009) dia is required for furrow canal formation during cellularisation. (Grosshans et al., 2005) dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells. (Eggert et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) dia has a role in actin cytoskeleton organisation. (Afshar et al., 2000) dia function is required for cytokinesis. (Castrillon and Wasserman, 1994) Proliferation defect locus. (Castrillon et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057633 NM_165341 NM_001259183 NM_001259184 NM_001273711 RefSeq (Proteins) • NP_476981 NP_724285 NP_001246112 NP_001246113 NP_001260640 DDBJ / EMBL / GenBank • AA142035 AA439629 AA536376 AAA67715 AAF53922 AAN11087 AAX33546 AC004759 AC007467 AFH03786 AFH03787 AGB93175 AI946333 AQ025819 AQ026407 BT021398 CL844426 L80120 U11288 Entrez Gene - A searchable database of RefSeq genes. • 35340 UniProtKB/Swiss-Prot • P48608
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Actin-binding FH2/DRF autoregulatory (IPR003104) DRF autoregulatory (IPR010465) Diaphanous FH3 (IPR010472) Diaphanous autoregulatory (IPR014767) GTPase-binding/formin homology 3 (IPR014768) Actin-binding FH2 (IPR015425) Diaphanous GTPase-binding (IPR010473) Armadillo-type fold (IPR016024)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61303 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011202 DroID - A comprehensive database of gene and protein interactions. • FBgn0011202 DRSC - Results from RNAi screens. • FBgn0011202 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011202 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1768-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011202 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35340 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/diaph1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35340 modMine - Data generated by the modENCODE project. • FBgn0011202
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	38E.16 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) CG1768 (Goldstein and Gunawardena, 2000, Rogers et al., 2003, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1, Ni et al., 2011.7.19, Howell et al., 2012) Dia (Pedra et al., 2004, Webb et al., 2007, Homem and Peifer, 2007, Matusek et al., 2006, Padash Barmchi et al., 2005, Bakal et al., 2007, Homem and Peifer, 2008, Mulinari et al., 2008, Muyskens and Guillemin, 2011, Cavey et al., 2008, Hickson and O'Farrell, 2008, Stevens et al., 2008, Llense and Martín-Blanco, 2008, Price et al., 2006, Warner and Longmore, 2009, Laplante et al., 2010, Warner and Longmore, 2009, Zavortink et al., 2005, Blanchard et al., 2010, Laplante and Nilson, 2011, Förster and Luschnig, 2012) dia (Janody and Treisman, 2006, Padash Barmchi et al., 2005, Hickson et al., 2006, Kuranaga et al., 2006, Pichaud et al., 2007, Sandmann et al., 2007, Stuart et al., 2007, Kadandale et al., 2010, Bakal et al., 2007, Guan et al., 2011, Delon and Brown, 2009, Vlachos and Harden, 2011, Levayer et al., 2011, Neisch et al., 2010, Peng et al., 2012, Corrigall et al., 2007, Homem and Peifer, 2008, Wang and Riechmann, 2007, Mulinari et al., 2008, Castellanos et al., 2008, Williams et al., 2007, Pawson et al., 2008, Verdier et al., 2006, Webb et al., 2009, Widmann and Dahmann, 2009, Massarwa et al., 2009, Bertet et al., 2009, Homem and Peifer, 2009, Cao et al., 2010, Matusek et al., 2008, Cosetti et al., 2008, Tucker et al., 2011, Wasbrough et al., 2010, Wasbrough et al., 2010, Neumüller et al., 2011, Howell et al., 2012) DIA (D'Avino et al., 2005) Dias (Bertet et al., 2009) l(2)k07135 (BDGP Project Members, 1994-1999, ) ms(2)04138
Name Synonym	diaphanous (Pichaud, 2007, Padash Barmchi et al., 2005, Gregory et al., 2007, Hoopfer et al., 2008, Pichaud et al., 2007, Lecuit, 2004, Dean et al., 2005, Guan et al., 2011, Silverman-Gavrila et al., 2008, Wang and Riechmann, 2007, Minin et al., 2006, Castellanos et al., 2008, Williams et al., 2007, Pawson et al., 2008, Verdier et al., 2006, Shalaby et al., 2009, Cao et al., 2010, Cosetti et al., 2008, Schoen et al., 2010, Wasbrough et al., 2010) Diaphanous (Webb et al., 2007, Homem and Peifer, 2007, Rogers et al., 2003, Prokopenko and Chia, 2007, Higgs and Peterson, 2005, Homem and Peifer, 2008, Prokopenko and Chia, 2008, Castoreno et al., 2010, Delon and Brown, 2009, Cavey et al., 2008, Cao et al., 2008, Homem and Peifer, 2008, Mulinari et al., 2008, Price et al., 2006, Zavortink et al., 2005, Webb et al., 2009, Homem and Peifer, 2009, Neisch et al., 2010, Blanchard et al., 2010, Laplante and Nilson, 2011)
Secondary FlyBase IDs
	FBgn0022100
References ( 174 )
Generate a list of	All references relating to this gene ( 174 )
List References by type	Research paper  ( 92 ) Supplementary material  ( 16 ) Review  ( 25 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 21 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Conference report  ( 1 )
Recent research papers ( 15 )
	Defays and Bertoli, 2012, Alcohol 46(8): 737--745 Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. [FBrf0220080] Dekanty et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(50): 20549--20554 Aneuploidy-induced delaminating cells drive tumorigenesis in Drosophila epithelia. [FBrf0220285] Förster and Luschnig, 2012, Nat. Cell Biol. 14(5): 526--534 Src42A-dependent polarized cell shape changes mediate epithelial tube elongation in Drosophila. [FBrf0218206] Howell et al., 2012, Immunogenetics 64(2): 155--161 A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response. [FBrf0217123] Palgi et al., 2012, BMC Genomics 13: 134 Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes. [FBrf0218515] Peng et al., 2012, Dev. Cell 23(3): 507--518 Planar Polarized Protrusions Break the Symmetry of EGFR Signaling during Drosophila Bract Cell Fate Induction. [FBrf0219458] Rojas-Ríos et al., 2012, PLoS Biol. 10(4): e1001298 Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. [FBrf0218031] Guan et al., 2011, Learn. Mem. 18(4): 191--206 Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. [FBrf0213277] Laplante and Nilson, 2011, J. Cell Biol. 192(2): 335--348 Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure. [FBrf0212838] Levayer et al., 2011, Nat. Cell Biol. 13(5): 529--540 Spatial regulation of Dia and Myosin-II by RhoGEF2 controls initiation of E-cadherin endocytosis during epithelial morphogenesis. [FBrf0213654] Muyskens and Guillemin, 2011, PLoS ONE 6(3): e17856 Helicobacter pylori CagA Disrupts Epithelial Patterning by Activating Myosin Light Chain. [FBrf0213299] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Sansores-Garcia et al., 2011, EMBO J. 30(12): 2325--2335 Modulating F-actin organization induces organ growth by affecting the Hippo pathway. [FBrf0213953] Tucker et al., 2011, Dis. Model Mech. 4(1): 126--134 Ena drives invasive macrophage migration in Drosophila embryos. [FBrf0212563] Vlachos and Harden, 2011, Genetics 187(2): 501--512 Genetic Evidence for Antagonism Between Pak Protein Kinase and Rho1 Small GTPase Signaling in Regulation of the Actin Cytoskeleton During Drosophila Oogenesis. [FBrf0212983] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...