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Gene Dmel\dia

General Information
Symbol	Dmel\dia	Species	D. melanogaster
Name	diaphanous	Annotation symbol	CG1768
Feature type	protein_coding_gene	FlyBase ID	FBgn0011202
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	38E.16
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	38E7-38E8	Sequence location	2L:20,758,147..20,767,536 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene diaphanous is referred to in FlyBase by the symbol Dmel\dia (CG1768, FBgn0011202). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence for 19 unique biological process terms, many of which group under: cell cycle; anatomical structure development; cellular component organization or biogenesis; multicellular organismal process; cell division; localization; biological regulation; cell cycle process; multicellular organismal reproductive process; cellular process involved in reproduction; apical constriction; embryonic development via the syncytial blastoderm; sensory perception of mechanical stimulus. 26 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; female germline cyst; portion of tissue; somatic cell; non-connected developing system; multi-cell-component structure; presumptive embryonic/larval nervous system; spermatocyte; cytoplasmic part; imaginal precursor; external compound sense organ; commissure; late extended germ band embryo. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Actin-binding FH2; Actin-binding FH2/DRF autoregulatory; Armadillo-type fold; DRF autoregulatory; Diaphanous FH3; Diaphanous GTPase-binding; Diaphanous autoregulatory; GTPase-binding/formin homology 3. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, larval/adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, larval/adult midgut, larval hindgut, larval/adult salivary gland, adult female reproductive system, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1625942_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of dia. Gene sequence location is 2L:20758147..20767536.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Bakal et al., 2007, Science 316(5832): 1753--1756 Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	References Laplante et al., 2010, Dev. Dyn. 239(9): 2509--2519 Sequence features BKN21796 HFA03519
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38E7-38E8   Limits computationally determined from genome sequence between P{PZ}Hr3802306&P{PZ}dia1 and P{EP}CG31673EP432
Experimentally Determined Cytological Location
Cytogenetic map Notes References 38E5-38E6   (determined by in situ hybridisation)   (Spradling et al., 1999) 38E1-38E10   38E5--6   (BDGP Project Members, 1994-1999) 38A1-39E1   Location based on deficiency mapping.   (Giansanti et al., 2004)
Experimentally Determined Recombination Data
Location	2- (Giansanti et al., 2004)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\dia for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) dia-RA FBtr0081410  NM_057633   4740   1091 dia-RB FBtr0081411  NM_165341   4243   1091 dia-RC FBtr0305960     1775   130 dia-RD FBtr0305961     3797   1098
Additional Transcript Data & Comments
Reported size (kB)	4.8, 4.4 (northern blot) (Castrillon and Wasserman, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein dia-PA   FBpp0080940   123.2   1091   8.25     NP_476981   AAF53922 dia-PB   FBpp0080941   123.2   1091   8.25     NP_724285   AAN11087 dia-PC   FBpp0297102   14.5   130   10.05       dia-PD   FBpp0297103   124.0   1098   8.51
Additional Polypeptide Data & Comments
Reported size (kDa)	1091 (aa); 123 (kD predicted) (Castrillon and Wasserman, 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Actin-binding FH2/DRF autoregulatory (IPR003104) DRF autoregulatory (IPR010465) Diaphanous FH3 (IPR010472) Diaphanous autoregulatory (IPR014767) GTPase-binding/formin homology 3 (IPR014768) Actin-binding FH2 (IPR015425) Diaphanous GTPase-binding (IPR010473) Armadillo-type fold (IPR016024)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA142035   AA392355   AA439629   AA441512   AA536376   AA802096   AE014134 AAF53922   AE014134 AAN11087   AC004759   AC007467   AI257364   AI293781   AI515699   AI946333   AQ025819   AQ026407   BG636029   BG641469   BI216639   BI239230   BI638524   BI638842   BT021398 AAX33546   BT120071   BT120072   BT120147   CK657499   CL844426   CO264241   CO268943   CO275614   CO289588   CO290616   CO293622   DN154231   EC061518   EC063745   EC068732   EC068821   EC070384   EC074922   EC082535   EC086747   EC197186   EC199931   EC207707   EC225351   EC231940   L80120   U11288 AAA67715
UniProtKB/Swiss-Prot	P48608
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage | female     (Castrillon and Wasserman, 1994) adult stage | male     (Castrillon and Wasserman, 1994)
Additional Descriptive Data
	dia transcripts are expressed in both males and females and are not germline specific. (Castrillon and Wasserman, 1994)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0011202    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0011202 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages, during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0011202 expression level Developmental Stage   Expression Level embryo 00-02hr    1702 embryo 02-04hr    1430 embryo 04-06hr    1744 embryo 06-08hr    1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr    1752 embryo 20-22hr    1644 embryo 22-24hr    1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6    1433 larva L3 puffstage 7-9    1392 white prepupae new    1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1702) embryo 02-04hr  (1430) embryo 04-06hr  (1744) embryo 06-08hr  (1520) embryo 08-10hr  (1330) embryo 10-12hr  (1066) embryo 12-14hr  (1118) embryo 14-16hr  (1040) embryo 16-18hr  (1267) embryo 18-20hr  (1752) embryo 20-22hr  (1644) embryo 22-24hr  (1933) larva L1  (742) larva L2  (645) larva L3 12hr old  (755) larva L3 puffstage 1-2  (955) larva L3 puffstage 3-6  (1433) larva L3 puffstage 7-9  (1392) white prepupae new  (1609) white prepupae 12hr  (835) white prepupae 24hr  (796) pupae 2d postWPP  (718) pupae 3d postWPP  (721) pupae 4d postWPP  (801) adult male 01day  (689) adult male 05day  (731) adult male 30day  (699) adult female 01day  (894) adult female 05day  (1363) adult female 30day  (1382) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1702) embryo 02-04hr  1430 embryo 04-06hr  (1744) embryo 06-08hr  (1520) embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr  (1752) embryo 20-22hr  (1644) embryo 22-24hr  (1933) larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6  1433 larva L3 puffstage 7-9    1392 white prepupae new  (1609) white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1702 embryo 02-04hr    1430 embryo 04-06hr    1744 embryo 06-08hr    1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr    1752 embryo 20-22hr    1644 embryo 22-24hr    1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6    1433 larva L3 puffstage 7-9    1392 white prepupae new    1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1702 embryo 02-04hr    1430 embryo 04-06hr    1744 embryo 06-08hr    1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr    1752 embryo 20-22hr    1644 embryo 22-24hr    1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6    1433 larva L3 puffstage 7-9    1392 white prepupae new    1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0011202 expression level Developmental Stage   Expression Level embryo 00-02hr    1702 embryo 02-04hr    1430 embryo 04-06hr    1744 embryo 06-08hr    1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr    1752 embryo 20-22hr    1644 embryo 22-24hr    1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6    1433 larva L3 puffstage 7-9    1392 white prepupae new    1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1702) embryo 02-04hr  (1430) embryo 04-06hr  (1744) embryo 06-08hr  (1520) embryo 08-10hr  (1330) embryo 10-12hr  (1066) embryo 12-14hr  (1118) embryo 14-16hr  (1040) embryo 16-18hr  (1267) embryo 18-20hr  (1752) embryo 20-22hr  (1644) embryo 22-24hr  (1933) larva L1  (742) larva L2  (645) larva L3 12hr old  (755) larva L3 puffstage 1-2  (955) larva L3 puffstage 3-6  (1433) larva L3 puffstage 7-9  (1392) white prepupae new  (1609) white prepupae 12hr  (835) white prepupae 24hr  (796) pupae 2d postWPP  (718) pupae 3d postWPP  (721) pupae 4d postWPP  (801) adult male 01day  (689) adult male 05day  (731) adult male 30day  (699) adult female 01day  (894) adult female 05day  (1363) adult female 30day  (1382) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1702 embryo 02-04hr  1430 embryo 04-06hr  1744 embryo 06-08hr  1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr  1752 embryo 20-22hr  1644 embryo 22-24hr  1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6  1433 larva L3 puffstage 7-9    1392 white prepupae new  1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1702 embryo 02-04hr    1430 embryo 04-06hr    1744 embryo 06-08hr    1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr    1752 embryo 20-22hr    1644 embryo 22-24hr    1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6    1433 larva L3 puffstage 7-9    1392 white prepupae new    1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1702 embryo 02-04hr    1430 embryo 04-06hr    1744 embryo 06-08hr    1520 embryo 08-10hr    1330 embryo 10-12hr    1066 embryo 12-14hr    1118 embryo 14-16hr    1040 embryo 16-18hr    1267 embryo 18-20hr    1752 embryo 20-22hr    1644 embryo 22-24hr    1933 larva L1    742 larva L2    645 larva L3 12hr old    755 larva L3 puffstage 1-2    955 larva L3 puffstage 3-6    1433 larva L3 puffstage 7-9    1392 white prepupae new    1609 white prepupae 12hr    835 white prepupae 24hr    796 pupae 2d postWPP    718 pupae 3d postWPP    721 pupae 4d postWPP    801 adult male 01day    689 adult male 05day    731 adult male 30day    699 adult female 01day    894 adult female 05day    1363 adult female 30day    1382 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0011202    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0011202 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, larval/adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, larval/adult midgut, larval hindgut, larval/adult salivary gland, adult female reproductive system, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0011202 expression level Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules  513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop  531.6 Adult Midgut    431.4 Adult Hindgut  (783.1) Adult Malpighian Tubules  (640.2) Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0011202 expression level Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules  513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop  531.6 Adult Midgut    431.4 Adult Hindgut  783.1 Adult Malpighian Tubules  640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    28.9 Larval Midgut    148.1 Larval Hindgut    189.1 Larval Malpighian Tubules    513.2 Larval Fat Body    68.6 Larval Salivary Gland    140.1 Larval Trachea    76.6 Larval Carcass    77.85 Adult Head    164.7 Adult Eye    39.425 Adult Brain    150.4 Adult Thoracic-Abdominal Ganglion    137.2 Adult Crop    531.6 Adult Midgut    431.4 Adult Hindgut    783.1 Adult Malpighian Tubules    640.2 Adult Fat Body    38.2 Adult Salivary Gland    102.7 Adult Heart    43.3 Adult VirginFemale Spermatheca    75.2 Adult InseminatedFemale Spermatheca    117.6 Adult Ovary    263.2 Adult Testis    45.4 Adult Male Accessory Gland    126.9 Adult Carcass    81.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    164.7   NA  Eye    39.425   NA  Brain    150.4   28.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    137.2   NA  Crop    531.6   148.1  Midgut    431.4   189.1  Hindgut    783.1   513.2  Malpighian Tubules    640.2   68.6  Fat Body    38.2   140.1  Salivary Gland    102.7   NA  Heart    43.3   76.6  Trachea    NA   NA  VirginFemale Spermatheca    75.2   NA  InseminatedFemale Spermatheca    117.6   NA  Ovary    263.2   NA  Testis    45.4   NA  Male Accessory Gland    126.9   77.85  Carcass    81.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011202 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1768-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 diaGD9442 diaKK101745 lethal | embryonic stage | non-rescuable maternal effect | recessive dia5 lethal | maternal effect | recessive dia9 lethal | pupal stage, with Scer\GAL4ph-p-NP0761 diaCA.Scer\UAS.T:Ivir\HA1 lethal | pupal stage | recessive dia2 dia3 lethal | recessive dia3 dia5 dia9 dia38 dia40 dia50 dia55 diak07135 diak07140 semi-lethal, with Scer\GAL4ph-p-NP1046 diaCA.Scer\UAS.T:Ivir\HA1 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 diaGD9442 viable diaEY11231 Sterility Allele female sterile | recessive dia1 fertile diaEY11231 male sterile dia1 male sterile | recessive dia1 Other Phenotypes Allele auditory perception defective, with Scer\GAL4CG13795-NP1245 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective, with Scer\GAL4JO15 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective, with Scer\GAL4NP6303 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective, with Scer\GAL4ph-p-NP1046 diaCA.Scer\UAS.T:Ivir\HA1 auditory perception defective | dominant dia5 cell shape defective | extended germ band stage diaCA.Scer\UAS.T:Ivir\HA1 cytokinesis defective | male (with Df(2L)TW84) diaZ5930 cytokinesis defective | male | recessive diaZ4053 diaZ5930 cytokinesis defective | recessive dia1 dia5 cytokinesis defective | somatic clone dia5 meiotic cell cycle defective | male (with Df(2L)TW84) diaZ5930 meiotic cell cycle defective | male | recessive diaZ4053 diaZ5930 mitotic cell cycle defective diadsRNA.cHa mitotic cell cycle defective | male (with Df(2L)TW84) diaZ5930 mitotic cell cycle defective | male | recessive diaZ4053 neuroanatomy defective, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 neuroanatomy defective | larval stage, with Scer\GAL41407 diaGD9442 neurophysiology defective | dominant dia5 visible, with Scer\GAL4pnr-MD237 diaGD9442 wild-type dia+t11 Phenotype manifest in Allele actin filament & embryonic/larval tracheal system dia5 actin filament & embryonic/larval tracheal system, with Scer\GAL4btl.PS diaNIG.1768R diaGD9442 actin filament | embryonic stage | germline clone dia5 amnioserosa, with Scer\GAL4c381 diaCA.Scer\UAS.T:Ivir\HA1 amnioserosa, with Scer\GAL4en-e16E diaCA.Scer\UAS.T:Ivir\HA1 amnioserosa | maternal effect (with dia2) dia5 amnioserosa | maternal effect (with dia5) dia2 blastoderm embryo | germline clone dia5 border follicle cell, with Scer\GAL4slbo.2.6 diaCA.Scer\UAS.T:Ivir\HA1 centrosome | embryonic stage | germline clone dia5 commissure, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 connective, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 contractile ring & meiotic cell cycle | male dia1 dia3 diaZ5930 contractile ring & meiotic cell cycle | male (with Df(2L)TW84) diaZ5930 cortical actin cytoskeleton & syncytial blastoderm embryo | maternal effect dia5 diak07135 cycle 11 embryo | germline clone dia5 cyst cell dia1 cytoskeleton | embryonic stage | germline clone dia5 denticle belt | germline clone dia5 dorsal closure embryo | germline clone dia5 ectoderm | extended germ band stage diaCA.Scer\UAS.T:Ivir\HA1 egg chamber dia1 egg chamber | somatic clone dia5 embryonic/first instar larval cuticle | germline clone dia5 embryonic/larval dorsal trunk, with Scer\GAL4btl.PS diaCA.Scer\UAS.T:Ivir\HA1 embryonic/larval dorsal trunk, with Scer\GAL4en-e16E diaCA.Scer\UAS.T:Ivir\HA1 embryonic/larval dorsal trunk, with Scer\GAL4prd.RG1 diaCA.Scer\UAS.T:Ivir\HA1 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 diaGD9442 embryonic epidermis, with Scer\GAL4en-e16E diaCA.Scer\UAS.T:Ivir\HA1 embryonic epidermis, with Scer\GAL4prd.RG1 diaCA.Scer\UAS.T:Ivir\HA1 embryonic head | germline clone dia5 epithelial cell | somatic clone, with Scer\GAL4Act5C.PI diaCA.Scer\UAS.T:Ivir\HA1 epithelium | somatic clone dia5 eye dia9 follicle cell dia1 follicle cell | somatic clone dia5 furrow canal | germline clone dia5 ganglion mother cell | larval stage, with Scer\GAL41407 diaGD9442 gastrula embryo | germline clone dia5 imaginal disc dia2 dia3 larval central nervous system & neuroblast dia2 dia3 dia9 mesothoracic tergum, with Scer\GAL4pnr-MD237 diaGD9442 microtubule | embryonic stage | germline clone dia5 morphogenetic furrow | somatic clone dia5 Nebenkern dia1 diaZ4053 diaZ5930 Nebenkern (with Df(2L)TW84) diaZ5930 nucleus | embryonic stage | germline clone dia5 organism | dorsal closure stage, with Scer\GAL4c381 diaCA.Scer\UAS.T:Ivir\HA1 organism | dorsal closure stage | maternal effect (with dia2) dia5 organism | dorsal closure stage | maternal effect (with dia5) dia2 pole bud | germline clone dia5 pole cell | germline clone dia5 presumptive embryonic/larval central nervous system, with Scer\GAL4elav-C155 diaCA.Scer\UAS.T:Ivir\HA1 S2 cell-line & spindle microtubule diadsRNA.cHa spermatid dia1 spermatid & nucleus | supernumerary diaZ4053 diaZ5930 spermatid & nucleus | supernumerary (with Df(2L)TW84) diaZ5930 spermatocyte dia1 spindle & meiotic cell cycle | male dia3 diaZ5930 spindle & meiotic cell cycle | male (with Df(2L)TW84) diaZ5930 testis dia1 tracheal fusion cell, with Scer\GAL4btl.PS diaCA.Scer\UAS.T:Ivir\HA1 ventral furrow | non-rescuable maternal effect dia5
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of dia Class Mutagen Stocks Known lesion diak07135 P-element activity 3 -- dia1 hypomorphic allele - genetic evidence P-element activity 2 Yes dia5 loss of function allele, hypomorphic allele - genetic evidence Delta2-3 1 Yes diaGS2135 1 Yes diak07140 P-element activity 1 -- dia2 amorphic allele - genetic evidence Delta2-3 0 Yes dia38 ethyl methanesulfonate 0 -- dia3 hypomorphic allele - genetic evidence Delta2-3 0 Yes dia40 ethyl methanesulfonate 0 -- dia50 ethyl methanesulfonate 0 -- dia55 ethyl methanesulfonate 0 -- dia9 hypomorphic allele - genetic evidence Delta2-3 0 Yes diaEY11231 P-element activity 0 -- diaunspecified 0 -- diaZ4053 ethyl methanesulfonate 0 -- diaZ5930 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of dia Class Mutagen Stocks Known lesion diaCA.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion 1 Yes diaGD9442 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaGL00408 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaHM05027 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaHMS00308 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes diaKK101745 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes dia+t11 in vitro construct - genomic fragment 0 Yes diadsRNA.cHa in vitro construct - RNAi 0 -- diaNIG.1768R in vitro construct - RNAi 0 -- diaScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)DS5 (Butler et al., 2001) Df(2L)DS6 (Kozlova et al., 1998) Df(2L)DS9 (Kozlova et al., 1998, Butler et al., 2001) Df(2L)Fs(2)Ket-RX32 (Kozlova et al., 1998, Butler et al., 2001) Df(2L)pr1122 (Butler et al., 2001) Df(2L)pr9204 (Butler et al., 2001) Df(2L)pr11163 (Butler et al., 2001) Df(2L)TW2 (Butler et al., 2001) Df(2L)TW65 (Butler et al., 2001) Df(2L)TW84 (Butler et al., 2001, Giansanti et al., 2004, Castrillon et al., 1993, BDGP Project Members, 1994-1999, Castrillon and Wasserman, 1994) Df(2L)TW161 (BDGP Project Members, 1994-1999) Df(2L)VA6 (Butler et al., 2001, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2L)Acon-21 (Butler et al., 2001) Df(2L)DS8 (Butler et al., 2001) Df(2L)pr1 (Butler et al., 2001) Df(2L)pr2b (Butler et al., 2001) Df(2L)pr37 (Butler et al., 2001) Df(2L)pr9201 (Butler et al., 2001) Df(2L)pr-A16 (Butler et al., 2001) Df(2L)TW50 (Butler et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9442} NA P{KK101745} NA P{TRiP.GL00408} NA P{TRiP.HM05027} NA P{TRiP.HMS00308} NA P{UAS-dia.CA} NA P{UASp-dia.EGFP} NA characterization construct P{DC4} NA P{NIG.1768R} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}diak07135 No P{lacW}diak07140 No P{lacW}l(2)SH0694SH0694 No P{PZ}dia1 No insertion of mobile activating element P{EPgy2}diaEY11231 NA P{GSV1}diaGS2135 NA miscellaneous insertions P{?PZ}dia5 NA
Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )
Terms Based on Experimental Evidence ( 26 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Apc2 (Webb et al., 2009) inferred from physical interaction with chic (Webb et al., 2009) inferred from physical interaction with Eb1 (Webb et al., 2009)
Biological Process
CV term Evidence References
actin filament organization inferred from mutant phenotype (Afshar et al., 2000, Kiger et al., 2003, Grosshans et al., 2005) apical constriction inferred from mutant phenotype (Bertet et al., 2009) cellularization inferred from mutant phenotype (Afshar et al., 2000, Grosshans et al., 2005) cytokinesis inferred from mutant phenotype (Kiger et al., 2003, Rogers et al., 2003, Echard et al., 2004, Eggert et al., 2004, Dean et al., 2005) cytokinesis, actomyosin contractile ring assembly inferred from mutant phenotype (Giansanti et al., 2004) cytokinesis after meiosis I inferred from mutant phenotype (Giansanti et al., 2004) cytokinesis after meiosis II inferred from mutant phenotype (Giansanti et al., 2004) maintenance of protein location in cell inferred from mutant phenotype (Dean et al., 2005) mitotic cell cycle, embryonic inferred from mutant phenotype (Afshar et al., 2000) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) open tracheal system development inferred from mutant phenotype (Matusek et al., 2006) pole cell formation inferred from mutant phenotype (Afshar et al., 2000) protein localization inferred from mutant phenotype (Afshar et al., 2000) protein secretion inferred from mutant phenotype (Massarwa et al., 2009) pseudocleavage involved in syncytial blastoderm formation inferred from mutant phenotype (Webb et al., 2009) regulation of embryonic cell shape inferred from mutant phenotype (Homem and Peifer, 2008, Bertet et al., 2009) sensory perception of sound inferred from mutant phenotype (Cosetti et al., 2008, Schoen et al., 2010) spermatogenesis inferred from mutant phenotype (Castrillon et al., 1993) spindle assembly inferred from mutant phenotype (Giansanti et al., 2004)
Cellular Component
CV term Evidence References
colocalizes_with actin filament inferred from direct assay (Webb et al., 2009) adherens junction inferred from direct assay (Homem and Peifer, 2008) apical part of cell inferred from direct assay (Massarwa et al., 2009, Corrigall et al., 2007) cell cortex inferred from direct assay (Homem and Peifer, 2008) cleavage furrow inferred from direct assay (Homem and Peifer, 2008) protein complex inferred from physical interaction with Apc2 (Webb et al., 2009)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
actin binding inferred from electronic annotation with InterPro:IPR003104, InterPro:IPR010472, InterPro:IPR010473 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) Rho GTPase binding inferred from electronic annotation with InterPro:IPR010473 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
actin filament-based process traceable author statement (Van Aelst and Symons, 2002, Mazumdar and Mazumdar, 2002) cellularization traceable author statement (Tepass et al., 2001, Mazumdar and Mazumdar, 2002) cytokinesis non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) traceable author statement (Van Aelst and Symons, 2002, Mazumdar and Mazumdar, 2002) cytokinesis, actomyosin contractile ring assembly non-traceable author statement (Tepass et al., 2001) protein localization non-traceable author statement (Gonzalez and Bornens, 2000) traceable author statement (Mazumdar and Mazumdar, 2002)
Cellular Component
CV term Evidence References
actomyosin contractile ring non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions dia allele Gene References dia1 CycB (Ji et al., 2002) LIMK1 (Chen et al., 2004) dia2 CycB (Ji et al., 2002) pbl (Prokopenko et al., 1999) Rho1 (Prokopenko et al., 1999) dia5 LIMK1 (Ng and Luo, 2004) dia9 CycB (Ji et al., 2002) diak07135 LIMK1 (Chen et al., 2004) unspecified pbl (Gregory et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61303 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011202 DroID - A comprehensive database of gene and protein interactions. • FBgn0011202 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35340
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15354 Dere\GG21250 Dgri\GH11618 Dmoj\GI17921 Dper\GL18518 Dpse\GA14608 Dsec\GM23367 Dsim\GD24279 Dvir\GJ17693 Dwil\GK14913 Dyak\GE12345
Curated drosophilid orthologs	Dpse\GA14608
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL014868-PA Aedes aegypti (mosquito) AGAP009643-PA Anopheles gambiae (mosquito) XP_395654.3 Apis mellifera (honey bee) XP_001943564.1 Acyrthosiphon pisum (pea aphid) 73298 Branchiostoma floridae (lancelet) XP_001893363.1 Brugia malayi (roundworm nematode) BGIBMGA011175-PA Bombyx mori (silk worm) ENSBTAP00000023082 Bos taurus (bovine) CAL0006256 Candida albicans (a form of yeast) CN01316 Caenorhabditis brenneri (small nematode) CBP36383 Caenorhabditis briggsae (nematode) CE36536 Caenorhabditis elegans (worm) ENSCAFP00000009242 Canis familiaris (dog) ENSCINP00000003491 Ciona intestinalis (sea squirt) JA08562 Caenorhabditis japonica (nematode) cn10108 Cryptococcus neoformans (yeast-like fungus) CPIJ000980 Culex pipens (mosquito) ENSCPOP00000006697 Cavia porcellus (guinea pig) RP43164 Caenorhabditis remanei (nematode) ENSCSAVP00000013802 Ciona savignyi (sedentary tunicate) 225699 Capitella spl (polychaete worm) DDB0214996 Dictyostelium discoideum (amoeba) DEHA2E14366g Debaryomyces Hansenii (yeast) 220427 Daphnia pulex (daphnia) ENSDARP00000094075 Danio rerio (zebra fish) ENSECAP00000017323 Equus caballus (horse) XP_656030 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000023647 Gymnopilus aculeatus (mushroom) ENSGALP00000011053 Gallus gallus (chicken) 86808 Hypsipyla robusta (cedar shoot borer) ENSP00000348082 Homo sapiens (human) KLLA0C02321g Kluyveromyces lactis (yeast) 142208 Lottia gigantea (owl limpet) 24127 Monocosta brevicollis (choanoflagellate) ENSMODP00000002970 Monodelphis domestica (gray opossum) ENSMMUP00000027456 Macaca mulatta (rhesus monkey) ENSMUSP00000094053 Mus musculus (house mouse) 171940 Nematostella vectensis (starlet anemone) XP_001600271.1 Nasonia vitripennis (wasp) ENSOANP00000011150 Ornithorynchus anatinus (platypus) ENSORLP00000005173 Oryzias latipes (japanese killifish) PHUM001551-PA Pediculus humanus (human body louse) ENSPPYP00000022986 Pongo pygmaeus (orangutan) 134532 Phytophthora sojae (root rot pathogen) 242488 Populus trichocarpa (black cottonwood) ENSPTRP00000010055 Pan troglodytes (chimpanzee) ENSRNOP00000026785 Rattus norvegicus (norway rat) YIL159W Saccharomyces cerevisiae (budding yeast) Smp_068720 Schistosoma mansoni (trematode parasite) SPCC895.05 Schizosaccharomyces pombe (fission yeast) XP_001184278.1 Strongylocentrotus purpuratus (purple sea urchin) 60005 Trichoplax adhaerens (metazoa) XP_971816.1 Tribolium castaneum (red flour beetle) ENSTNIP00000008593 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000028358 Takifugu rubripes (pufferfish Takifugu) 95394.m00365 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000039333 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV16014 LH10922 FBgn0230095 FBgn0216918 FBgn0204862 FBgn0195627 FBgn0178234 JGI_V11_220427 FBgn0074635 FBgn0156119 FBgn0140662 FBgn0119098 FBgn0113430 FBgn0092379 CPIJ000980 BGIBMGA011175 ACYPI002122 GB12036 AGAP009643 AAEL014868 AAEL012283 PB21742 PHUM368970 GLEAN_06029
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	27616 w*; P{UAS-dia.CA}3 9138 w*; dia5 P{neoFRT}40A/CyO 28541 y1 v1; P{TRiP.HM05027}attP2 33424 y1 sc* v1; P{TRiP.HMS00308}attP2 35479 y1 sc* v1; P{TRiP.GL00408}attP40/CyO
Kyoto	108878 P{PZ}dia1 cn1/CyO; ry506 108859 y1 w67c23; P{lacW}diak07135/CyO 205048 y1 w67c23; P{GSV1}diaGS2135/SM1 102532 y1 w67c23 ; P{lacW}diak07135 / CyO 102535 y1 w67c23 ; P{lacW}diak07140 / CyO
VDRC	v103914 P{KK101745}VIE-260B v20518 w1118; P{GD9442}v20518
	More stocks available...
Genomic Clones ( 1 )
	BACR07D17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD14246
Other clones	DPiM_LD14246 MIP15714 MIP15814 MIP15914
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM03848 LD11226 LD16477 LD16963 LD41344 LP05703 LP06996 LP23848 RE23844 RE35666 SD12820 SD13607 SD20829 SD21236
Other clones	34943 53725 69522 73490 74408 103116 bs23g02 CK01.20D.C7 CK01.22A.F6 CK01.91B.C5 CK01.95D.D8 CK01.121D.C3 CK01.121D.C12 CK00152 EK071411 EK074534 EK093142 EK118048 EK138303 EK202451 EK203851
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011202 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35340
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: dia CG1768 (Lei, 1999.11)
Source for database merge of	Source for merge of: dia l(2)k07135 (Spradling et al., 1999)
Additional comments	null alleles lethal.
Other Comments
	dia is required for generation of apical F-actin in diverse types of epithelial tubes in the embryo. (Massarwa et al., 2009) dia is required for furrow canal formation during cellularisation. (Grosshans et al., 2005) dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells. (Eggert et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) dia has a role in actin cytoskeleton organisation. (Afshar et al., 2000) dia function is required for cytokinesis. (Castrillon and Wasserman, 1994) Proliferation defect locus. (Castrillon et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057633 NM_165341 RefSeq (Proteins) • NP_476981 NP_724285 DDBJ / EMBL / GenBank • AA142035 AA439629 AA536376 AAA67715 AAF53922 AAN11087 AAX33546 AC004759 AC007467 AI946333 AQ025819 AQ026407 BT021398 CL844426 L80120 U11288 Entrez Gene - A searchable database of RefSeq genes. • 35340 UniProtKB/Swiss-Prot • P48608
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Actin-binding FH2/DRF autoregulatory (IPR003104) DRF autoregulatory (IPR010465) Diaphanous FH3 (IPR010472) Diaphanous autoregulatory (IPR014767) GTPase-binding/formin homology 3 (IPR014768) Actin-binding FH2 (IPR015425) Diaphanous GTPase-binding (IPR010473) Armadillo-type fold (IPR016024)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61303 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011202 DroID - A comprehensive database of gene and protein interactions. • FBgn0011202 DRSC - Results from RNAi screens. • FBgn0011202 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011202 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1768-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011202 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35340 InParanoid A subset of ortholog calls from InParanoid. • AAEL014868-PA Aedes aegypti (mosquito) AGAP009643-PA Anopheles gambiae (mosquito) XP_395654.3 Apis mellifera (honey bee) XP_001943564.1 Acyrthosiphon pisum (pea aphid) 73298 Branchiostoma floridae (lancelet) XP_001893363.1 Brugia malayi (roundworm nematode) BGIBMGA011175-PA Bombyx mori (silk worm) ENSBTAP00000023082 Bos taurus (bovine) CAL0006256 Candida albicans (a form of yeast) CN01316 Caenorhabditis brenneri (small nematode) CBP36383 Caenorhabditis briggsae (nematode) CE36536 Caenorhabditis elegans (worm) ENSCAFP00000009242 Canis familiaris (dog) ENSCINP00000003491 Ciona intestinalis (sea squirt) JA08562 Caenorhabditis japonica (nematode) cn10108 Cryptococcus neoformans (yeast-like fungus) CPIJ000980 Culex pipens (mosquito) ENSCPOP00000006697 Cavia porcellus (guinea pig) RP43164 Caenorhabditis remanei (nematode) ENSCSAVP00000013802 Ciona savignyi (sedentary tunicate) 225699 Capitella spl (polychaete worm) DDB0214996 Dictyostelium discoideum (amoeba) DEHA2E14366g Debaryomyces Hansenii (yeast) 220427 Daphnia pulex (daphnia) ENSDARP00000094075 Danio rerio (zebra fish) ENSECAP00000017323 Equus caballus (horse) XP_656030 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000023647 Gymnopilus aculeatus (mushroom) ENSGALP00000011053 Gallus gallus (chicken) 86808 Hypsipyla robusta (cedar shoot borer) ENSP00000348082 Homo sapiens (human) KLLA0C02321g Kluyveromyces lactis (yeast) 142208 Lottia gigantea (owl limpet) 24127 Monocosta brevicollis (choanoflagellate) ENSMODP00000002970 Monodelphis domestica (gray opossum) ENSMMUP00000027456 Macaca mulatta (rhesus monkey) ENSMUSP00000094053 Mus musculus (house mouse) 171940 Nematostella vectensis (starlet anemone) XP_001600271.1 Nasonia vitripennis (wasp) ENSOANP00000011150 Ornithorynchus anatinus (platypus) ENSORLP00000005173 Oryzias latipes (japanese killifish) PHUM001551-PA Pediculus humanus (human body louse) ENSPPYP00000022986 Pongo pygmaeus (orangutan) 134532 Phytophthora sojae (root rot pathogen) 242488 Populus trichocarpa (black cottonwood) ENSPTRP00000010055 Pan troglodytes (chimpanzee) ENSRNOP00000026785 Rattus norvegicus (norway rat) YIL159W Saccharomyces cerevisiae (budding yeast) Smp_068720 Schistosoma mansoni (trematode parasite) SPCC895.05 Schizosaccharomyces pombe (fission yeast) XP_001184278.1 Strongylocentrotus purpuratus (purple sea urchin) 60005 Trichoplax adhaerens (metazoa) XP_971816.1 Tribolium castaneum (red flour beetle) ENSTNIP00000008593 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000028358 Takifugu rubripes (pufferfish Takifugu) 95394.m00365 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000039333 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/diaph1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35340 modMine - Data generated by the modENCODE project. • FBgn0011202 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV16014 LH10922 FBgn0230095 FBgn0216918 FBgn0204862 FBgn0195627 FBgn0178234 JGI_V11_220427 FBgn0074635 FBgn0156119 FBgn0140662 FBgn0119098 FBgn0113430 FBgn0092379 CPIJ000980 BGIBMGA011175 ACYPI002122 GB12036 AGAP009643 AAEL014868 AAEL012283 PB21742 PHUM368970 GLEAN_06029
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	38E.16 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) CG1768 (Goldstein and Gunawardena, 2000, Rogers et al., 2003, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1, Ni et al., 2011.7.19) Dia (Pedra et al., 2004, Webb et al., 2007, Homem and Peifer, 2007, Matusek et al., 2006, Padash Barmchi et al., 2005, Bakal et al., 2007, Homem and Peifer, 2008, Mulinari et al., 2008, Cavey et al., 2008, Hickson and O'Farrell, 2008, Stevens et al., 2008, Llense and Martín-Blanco, 2008, Price et al., 2006, Warner and Longmore, 2009, Laplante et al., 2010, Warner and Longmore, 2009, Zavortink et al., 2005, Neisch et al., 2010, Blanchard et al., 2010, Laplante and Nilson, 2011) dia (Janody and Treisman, 2006, Padash Barmchi et al., 2005, Hickson et al., 2006, Kuranaga et al., 2006, Pichaud et al., 2007, Sandmann et al., 2007, Stuart et al., 2007, Kadandale et al., 2010, Bakal et al., 2007, Corrigall et al., 2007, Homem and Peifer, 2008, Wang and Riechmann, 2007, Mulinari et al., 2008, Castellanos et al., 2008, Williams et al., 2007, Pawson et al., 2008, Verdier et al., 2006, Webb et al., 2009, Widmann and Dahmann, 2009, Massarwa et al., 2009, Bertet et al., 2009, Homem and Peifer, 2009, Cao et al., 2010, Matusek et al., 2008, Cosetti et al., 2008, Tucker et al., 2011, Wasbrough et al., 2010, Wasbrough et al., 2010, Neumüller et al., 2011) DIA (D'Avino et al., 2005) Dias (Bertet et al., 2009) l(2)k07135 (BDGP Project Members, 1994-1999, ) ms(2)04138
Name Synonym	diaphanous (Pichaud, 2007, Padash Barmchi et al., 2005, Gregory et al., 2007, Hoopfer et al., 2008, Pichaud et al., 2007, Lecuit, 2004, Dean et al., 2005, Silverman-Gavrila et al., 2008, Wang and Riechmann, 2007, Minin et al., 2006, Castellanos et al., 2008, Williams et al., 2007, Pawson et al., 2008, Verdier et al., 2006, Shalaby et al., 2009, Cao et al., 2010, Cosetti et al., 2008, Schoen et al., 2010, Wasbrough et al., 2010) Diaphanous (Webb et al., 2007, Homem and Peifer, 2007, Rogers et al., 2003, Prokopenko and Chia, 2007, Higgs and Peterson, 2005, Homem and Peifer, 2008, Prokopenko and Chia, 2008, Cavey et al., 2008, Cao et al., 2008, Homem and Peifer, 2008, Mulinari et al., 2008, Price et al., 2006, Zavortink et al., 2005, Webb et al., 2009, Homem and Peifer, 2009, Neisch et al., 2010, Blanchard et al., 2010, Laplante and Nilson, 2011)
Secondary FlyBase IDs
	FBgn0022100
References ( 159 )
Generate a list of	All references relating to this gene ( 159 )
List References by type	Research paper  ( 79 ) Supplementary material  ( 15 ) Review  ( 25 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 21 ) FlyBase analysis  ( 2 ) Computer file  ( 3 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Conference report  ( 1 )
Recent research papers ( 11 )
	Laplante and Nilson, 2011, J. Cell Biol. 192(2): 335--348 Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure. [FBrf0212838] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Sansores-Garcia et al., 2011, EMBO J. 30(12): 2325--2335 Modulating F-actin organization induces organ growth by affecting the Hippo pathway. [FBrf0213953] Tucker et al., 2011, Dis. Model Mech. 4(1): 126--134 Ena drives invasive macrophage migration in Drosophila embryos. [FBrf0212563] Blanchard et al., 2010, Development 137(16): 2743--2752 Cytoskeletal dynamics and supracellular organisation of cell shape fluctuations during dorsal closure. [FBrf0211395] Cao et al., 2010, Curr. Biol. 20(8): 770--776 Cortical Actin Dynamics Facilitate Early-Stage Centrosome Separation. [FBrf0211162] Kadandale et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(23): 10502--10507 Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment. [FBrf0210996] Laplante et al., 2010, Dev. Dyn. 239(9): 2509--2519 Echinoid regulates tracheal morphology and fusion cell fate in Drosophila. [FBrf0211771] Neisch et al., 2010, J. Cell Biol. 189(2): 311--323 Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. [FBrf0210554] Schoen et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(30): 13396--13401 Increased activity of Diaphanous homolog 3 (DIAPH3)/diaphanous causes hearing defects in humans with auditory neuropathy and in Drosophila. [FBrf0211399] Wasbrough et al., 2010, J. Proteomics 73(11): 2171--2185 The Drosophila melanogaster sperm proteome-II (DmSP-II). [FBrf0211978] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...