FlyBase Gene Report: Dmel\bol

General Information

Symbol

Dmel\bol

Species

D. melanogaster

Name

boule

Annotation Symbol

CG4760

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011206

Gene Model Status

Current

Stock Availability

17 publicly available

Gene Summary

boule (bol) encodes a translational regulator required in spermatogenesis for entry into meiosis and spermatid differentiation. It has an additional role in mushroom body γ neurons as a negative regulator of axon pruning. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

lincRNA.485

Key Links

Genomic Location

Cytogenetic map

66F5-67A1

Sequence location

3L:9,097,629..9,134,405 [-]

Recombination map

3-28

RefSeq locus

NT_037436 REGION:9097629..9134405

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables RNA binding

inferred from mutant phenotype

(assigned by UniProt )

(Eberhart et al., 1996)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables mRNA 3'-UTR binding

inferred from biological aspect of ancestor with PANTHER:PTN000877352

(assigned by GO_Central )

(Gaudet et al., 2011)

enables mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

enables translation activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000877352

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in G2/MI transition of meiotic cell cycle

inferred from mutant phenotype

(assigned by UniProt )

(Eberhart et al., 1996)

involved_in regulation of female receptivity

inferred from mutant phenotype

(assigned by UniProt )

(Ram and Wolfner, 2007)

involved_in regulation of translation

inferred from genetic interaction with FLYBASE:twe; FB:FBgn0002673

(Maines and Wasserman, 1999)

involved_in spermatocyte division

inferred from mutant phenotype

(assigned by UniProt )

(Eberhart et al., 1996)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in 3'-UTR-mediated mRNA stabilization

inferred from biological aspect of ancestor with PANTHER:PTN000877352

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in G2/MI transition of meiotic cell cycle

traceable author statement

(Fuller, 1998)

involved_in positive regulation of translational initiation

inferred from biological aspect of ancestor with PANTHER:PTN000877352

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in spermatogenesis

traceable author statement

(Fuller, 1998)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Cheng et al., 1998)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Cheng et al., 1998)

located_in perikaryon

inferred from direct assay

(Hoopfer et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000877352

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the RRM DAZ family. (Q24207)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

RNA-binding protein that plays a central role in spermatogenesis. Required for meiotic entry and germline differentiation, at the transition between G2 and M phases of meiosis I. Acts by regulating translation of specific mRNAs, possibly by binding to their 3'-UTR. Essential for translation of twine (twe) mRNA. Required for the expression of various genes such as CG6784, CG17210, CG15841 scpr-B, scpr-C, and rho-6.

(UniProt, Q24207)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\bol for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Gene model reviewed during 5.51

Gene model reviewed during 5.46

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.45

Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

bol-RA

FBtr0076541

NM_168319

1801

189

bol-RB

FBtr0076538

NM_079265

2511

228

bol-RC

FBtr0076539

NM_168320

2294

189

bol-RD

FBtr0076540

NM_168318

2360

228

FBtr0300462

2023

FBtr0331858

6538

233

bol-RG

FBtr0344979

NM_001300063

2930

228

Additional Transcript Data and Comments

Reported size (kB)

3.0 (northern blot)

(Eberhart et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

bol-PA

FBpp0076268

20.2

189

8.83

NP_729457

AAF50316

bol-PB

FBpp0076265

24.7

228

7.65

NP_523989

AAF50317

bol-PC

FBpp0076266

20.2

189

8.83

NP_729458

AAN11987

bol-PD

FBpp0076267

24.7

228

7.65

NP_729456

AAN11986

bol-PE

FBpp0289689

9.9

7.53

NP_001163394

ACZ94665

bol-PF

FBpp0304242

25.2

233

7.14

NP_001261614

AGB94309

bol-PG

FBpp0311234

24.7

228

7.65

NP_001286992

AHN58017

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

228 aa isoforms: bol-PB, bol-PD, bol-PG

189 aa isoforms: bol-PA, bol-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

228 (aa)

(Eberhart et al., 1996)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with the translational regulator orb2.

(UniProt, Q24207)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bol using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

organism

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

late third instar larval stage

gamma Kenyon cell

(Hoopfer et al., 2008)

Kenyon cell | restricted

(Hoopfer et al., 2008)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage & pupal stage

(Eberhart et al., 1996)

spermatogenesis & adult stage

testis

(Eberhart et al., 1996)

Additional Descriptive Data

Zygotic-specific isoforms of bol with long 3' UTR extensions were observed.

(Hilgers et al., 2011)

bol mRNA is lost from the mushroom body gamma neurons by 0 hours APF.

(Hoopfer et al., 2008)

bol transcripts are expressed only in males where they are germline specific.

(Eberhart et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval brain

(Joiner and Wu, 2004)

late third instar larval stage

gamma Kenyon cell

(Hoopfer et al., 2008)

Kenyon cell | restricted

(Hoopfer et al., 2008)

adult stage

adult brain

(Joiner and Wu, 2004)

spermatogenesis & adult stage | male

spermatocyte

(Xu et al., 2012, Franklin-Dumont et al., 2007)

adult stage | male & spermatogenesis

spermatid

(Xu et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

bol is seen both in the cytoplasm and in a perinucleuar dot in spermatocytes, and in the cytoplasm of spermatids.

(Xu et al., 2012)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Cheng et al., 1998)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Cheng et al., 1998)

located_in perikaryon

inferred from direct assay

(Hoopfer et al., 2008)

Expression Deduced from Reporters

Reporter: PBac{810.P.FSVS-2}bol^CPTI004519

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

adult mushroom body | faint

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\bol in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\bol

View exonic expression by tissue for Dmel\bol

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39049/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011206

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011206

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011206

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4760

Flygut - An atlas of the Drosophila adult midgut

FBgn0011206

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 38 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of bol

Transgenic constructs containing regulatory region of bol

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(3L)29A6

(Castrillon et al., 1993)

Df(3L)BSC35

(Andrade and Cook, 2003.6.2)

Df(3L)BSC170

(Gresens et al., 2006.7.11)

Df(3L)BSC390

(Christensen et al., 2007.10.29)

Gene not disrupted in

Df(3L)AC1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

bol^GD10525

viable, with Scer\GAL4^Mef2.PR

bol^GD10525

viable, with Scer\GAL4^pnr-MD237

bol^GD10525

viable, with Scer\GAL4^tin.CΔ4

bol^GD10525

Sterility

Allele

fertile

bol^EY09688

male fertile

bol^gDNA-RP

male semi-sterile | progressive | heat sensitive

male sterile | recessive

Other Phenotypes

Allele

abnormal meiotic cell cycle

abnormal meiotic cell cycle | male

decreased fecundity | male

increased cell death | spermatogenesis

visible, with Scer\GAL4^en-e16E

bol^EP3659

visible, with Scer\GAL4^ey.PH

bol^EP3659

visible, with Scer\GAL4^hs.2sev

bol^EP3659

visible, with Scer\GAL4^pnr-MD237

bol^GD10525

Phenotype manifest in

Allele

chaeta, with Scer\GAL4^pnr-MD237

bol^GD10525

eye, with Scer\GAL4^ey.PH

bol^EP3659

eye, with Scer\GAL4^hs.2sev

bol^EP3659

gamma Kenyon cell | pupal stage, with Scer\GAL4^ey-OK107

gamma Kenyon cell | pupal stage, with Scer\GAL4^Tab2-201Y

bol^EP3659

germline cell | male

bol¹

Kenyon cell | pupal stage, with Scer\GAL4^ey-OK107

Kenyon cell | pupal stage, with Scer\GAL4^Tab2-201Y

Nebenkern

bol¹

nucleus | spermatogenesis

primary spermatocyte cyst

spermatid

bol^unspecified

spermatozoon

spermatozoon | decreased number

spindle | male

bol¹

testis

wing blade posterior compartment, with Scer\GAL4^en-e16E

bol^EP3659

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (33)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\BOLL

12 of 15

Yes

Hsap\DAZL

6 of 15

Yes

Hsap\DAZ1

5 of 15

Yes

Hsap\DAZ2

4 of 15

Yes

Hsap\DAZ3

4 of 15

Yes

Hsap\DAZ4

4 of 15

Yes

Hsap\CIRBP

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\RBM34

1 of 15

Hsap\RBM38

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (30)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Boll

11 of 15

Yes

Mmus\Dazl

6 of 15

Yes

Mmus\Cirbp

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (28)

Rnor\Boll

6 of 13

Yes

Rnor\Dazl

4 of 13

Yes

Rnor\LOC100911373

4 of 13

Yes

Rnor\LOC108348134

2 of 13

Yes

Rnor\Cirbp

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (22)

Xtro\dazl

8 of 12

Yes

Xtro\cirbp

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (29)

Drer\dazl

9 of 15

Yes

Drer\cirbpa

1 of 15

Yes

Drer\cirbpb

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\zgc:55733

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\daz-1

10 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (11)

Atha\AT1G20880

2 of 9

Yes

Atha\AT1G22330

2 of 9

Yes

Atha\AT1G33470

2 of 9

Yes

Atha\AT1G76460

2 of 9

Yes

Atha\AT1G78260

2 of 9

Yes

Atha\ARP1

1 of 9

Yes

Atha\AT1G22910

1 of 9

Yes

Atha\AT2G46780

1 of 9

Yes

Atha\AT3G06970

1 of 9

Yes

Atha\AT5G40490

1 of 9

Atha\RBP1

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (15)

Scer\CWC2

1 of 15

Yes

Scer\GBP2

1 of 15

Yes

Scer\HRB1

1 of 15

Yes

Scer\LHP1

1 of 15

Yes

Scer\MRN1

1 of 15

Yes

Scer\MUD1

1 of 15

Yes

Scer\NAB3

1 of 15

Yes

Scer\NAB6

1 of 15

Yes

Scer\NAM8

1 of 15

Yes

Scer\NGR1

1 of 15

Yes

Scer\NOP12

1 of 15

Yes

Scer\PUB1

1 of 15

Yes

Scer\TIF3

1 of 15

Yes

Scer\WHI3

1 of 15

Yes

Scer\WHI4

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FYO )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18412

Drosophila persimilis

Dper\GL15077

Drosophila willistoni

Dwil\GK24376

Drosophila virilis

Dvir\GJ12804

Drosophila mojavensis

Dmoj\GI16553

Drosophila grimshawi

Dgri\GH15900

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150CK6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015962

Glossina morsitans

Tsetse fly

Gmor\GMOY007448

Glossina morsitans

Tsetse fly

Gmor\GMOY007544

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_08413

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001684

Anopheles darlingi

American malaria mosquito

Adar\ADAC001933

Anopheles gambiae

Malaria mosquito

Agam\AGAP005506

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015150

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01KJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA013406

Danaus plexippus

Monarch butterfly

Dple\DPOGS203762-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002961

Apis florea

Little honeybee

Aflo\3118505860

Apis mellifera

Western honey bee

Amel\GB54094

Apis mellifera

Western honey bee

Amel\GB54095

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11153

Bombus terrestris

Buff-tailed bumblebee

Bter\15208974

Linepithema humile

Argentine ant

Lhum\LH22845

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0031ae

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0031af

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006679

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_11580

Tribolium castaneum

Red flour beetle

Tcas\bol

Pediculus humanus

Human body louse

Phum\PHUM575390

Rhodnius prolixus

Kissing bug

Rpro\RPRC005307

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI089294

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002644

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07509

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01II )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Stegodyphus mimosarum

African social velvet spider

Smim\KFM67886.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur24g00390

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g12860

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_299633

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02CS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Boule

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000010230

Gallus gallus

Domestic chicken

Ggal\DAZL

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (24)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

ssx

1 of 10

Sxl

1 of 10

tra2

1 of 10

trv

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BOLL; boule homolog, RNA binding protein

12 of 15

606165

DAZL; deleted in azoospermia like

6 of 15

601486

DAZ1; deleted in azoospermia 1

5 of 15

400003

DAZ2; deleted in azoospermia 2

4 of 15

400026

DAZ3; deleted in azoospermia 3

4 of 15

400027

DAZ4; deleted in azoospermia 4

4 of 15

400048

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

bol

Hsap\BOLL

(Xu et al., 2003)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

bol - Hsc70-4

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, pull down, Identification by mass spectrometry

(Gao et al., 2020)

bol - Hsp60C

pull down, Identification by mass spectrometry, anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Gao et al., 2020)

protein-protein

Physical Interaction

Assay

References

bol - CG9106

two hybrid

(Hu et al., 2017.6.13)

bol - orb2

anti bait coimmunoprecipitation, western blot

(Xu et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Gao et al., 2020)

suppressible

(Gao et al., 2020)

suppressible

(Maines and Wasserman, 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Zhu et al., 2005)

enhanceable

(Zhu et al., 2005)

enhanceable

(Zhu et al., 2005)

enhanceable

(Tuxworth et al., 2011)

Pvr

enhanceable

bol

(Zhu et al., 2005)

External Data

Subunit Structure (UniProtKB)

Interacts with the translational regulator orb2.

(UniProt, Q24207 )

Linkouts

BioGRID - A database of protein and genetic interactions.

64449

DroID - A comprehensive database of gene and protein interactions.

FBgn0011206

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-28

Cytogenetic map

66F5-67A1

Sequence location

3L:9,097,629..9,134,405 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66F5-67A1

Limits computationally determined from genome sequence between P{PZ}l(3)01629⁰¹⁶²⁹&P{PZ}mRpL12¹⁰⁵³⁴ and P{EP}Hsp26^EP3336&P{EP}Hsp26^EP3315

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66F1-66F6

(determined by in situ hybridisation)

(Spradling et al., 1999)

66F1-66F6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-28

(FlyBase, 2016)

3-21.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Bloomington

25759

y¹ w^*; P{UAS-bol.A::FLAG}12.2

65493

w¹¹¹⁸; PBac{IT.GAL4}bol^1179-G4

77620

w¹¹¹⁸; PBac{IT.GAL4}bol^2005-G4

56404

y¹ w^*; Mi{MIC}bol^MI10706

59500

y¹ w^*; Mi{MIC}bol^MI14335

11794

P{PZ}bol¹ ry⁵⁰⁶/TM6B, Tb¹

17591

y¹ w^67c23; P{EPgy2}bol^EY09688

28833

w^*; Pin¹/CyO; P{UAS-bol.A.ΔDAZ::FLAG}3.1

Kyoto

115626

w¹¹¹⁸; PBac{810.P.FSVS-2}bol^CPTI004519

101719

P{PZ}bol¹ ry⁵⁰⁶ / TM3, ry^RK Sb¹

VDRC

v101435

P{KK109153}VIE-260B

v21536

w¹¹¹⁸; P{GD10525}v21536

More stocks available...

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (114)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011206

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011206

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39049

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: bol BcDNA:AT13332

(FlyBase, 1996-)

Source for merge of: bol CG4727

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG4760 and CG4727 merged as CG4760 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of bol BcDNA:AT13332 was shared cDNA (AT13332) ( date:030602 ).

(FlyBase, 1996-)

Other Comments

The essential function of bol at the transition from G2 to M phase during meiosis is in the control of twe translation.

(Maines and Wasserman, 1999)

Meiotic entry failure in bol mutants can be overcome by heterologous expression of twe.

(Maines and Wasserman, 1999)

Rescue experiments suggest Xlae\dazl may play a role analogous to bol in promoting meiotic cell division.

(Houston et al., 1998)

bol has an essential meiotic function in spermatogenesis.

(Eberhart et al., 1996)

Meiotic entry defect.

(Castrillon et al., 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39049

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24207

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011206

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39049/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011206

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011206

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011206

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4760

Flygut - An atlas of the Drosophila adult midgut

FBgn0011206

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39049

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015063

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4760

MARRVEL_MODEL - MARRVEL (model organism gene)

39049

modMine - A data warehouse for the modENCODE project

FBgn0011206

Linkouts

BioGRID - A database of protein and genetic interactions.

64449

DroID - A comprehensive database of gene and protein interactions.

FBgn0011206

DRSC - Results frm RNAi screens

FBgn0011206

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011206

FlyMine - An integrated database for Drosophila genomics

FBgn0011206

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BOL

(Sofola et al., 2007)

BcDNA:AT13332

Bol

(Siddall and Hime, 2017, Xu et al., 2014, Franklin-Dumont et al., 2007)

Boule

(Lu and Fuller, 2015, Lesch and Page, 2012, Lim et al., 2012, Williams-Gregory et al., 2002)

CG4727

(Munier et al., 2004, Tseng and Hariharan, 2002, Morcillo, 2000.9.28)

CG4760

(Çiçek et al., 2016, Brooks et al., 2015, Misra et al., 2014, Keleman et al., 2009.8.5, Grieder et al., 2007, Cheng, 2000.4.18, Morcillo, 2000.9.28)

bol

(Lécureuil et al., 2021, Kurmangaliyev et al., 2020, Mazina et al., 2020, Shi et al., 2020, Fingerhut et al., 2019, Shih et al., 2019, Croset et al., 2018, Swevers et al., 2018, Hu et al., 2017.6.13, Ota et al., 2017, Carbone et al., 2016, Clandinin and Owens, 2016-, Brooks et al., 2015, Gene Disruption Project members, 2015-, Van Bortle et al., 2015, Ashwal-Fluss et al., 2014, Rodriguez et al., 2012, Smibert et al., 2012, Xu et al., 2012, Carreira et al., 2011, Hartmann et al., 2011, Hilgers et al., 2011, Moon et al., 2011, Gan et al., 2010, Kwon et al., 2009, Rautureau et al., 2009, Hoopfer et al., 2008, Turner et al., 2008, Chintapalli et al., 2007, Christensen et al., 2007.10.29, Grieder et al., 2007, Sofola et al., 2007, Arya et al., 2006, Gresens et al., 2006.7.11, Mukai et al., 2006, Joiner and Wu, 2004, Xu et al., 2003)

boule

(Tiwari et al., 2011, Shah et al., 2010)

lincRNA.485

(FlyBase Genome Annotators, 2014, Young et al., 2012)

ms(3)07272

Name Synonyms

Secondary FlyBase IDs

FBgn0040826
FBgn0063736

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (147)

Report Sections

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