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General Information
Symbol
Dmel\jar
Species
D. melanogaster
Name
jaguar
Annotation Symbol
CG5695
Feature Type
FlyBase ID
FBgn0011225
Gene Model Status
Stock Availability
Enzyme Name (EC)
Adenosinetriphosphatase (3.6.1.3)
Gene Snapshot
In progress.Contributions welcome.
Also Known As

Myosin VI, Mhc95F, 95F myosin, MyoVI, Myosin heavy chain at 95F

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:24,252,826..24,271,233 [-]
Recombination map

3-84

RefSeq locus
NT_033777 REGION:24252826..24271233
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. (Q01989)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O = ADP + phosphate (3.6.1.3)
Summaries
Gene Group (FlyBase)
MYOSINS -
Myosins are motor proteins which use the hydrolysis of ATP to drive movement along actin filaments. Myosins can be divided into two groups. Conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction. Unconventional myosins are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714).
Protein Function (UniProtKB)
Myosin is a protein that binds to actin and has ATPase activity that is activated by actin. Together CLIP-190 and jar may coordinate the interaction between the actin and microtubule cytoskeleton. May link endocytic vesicles to microtubules and may be involved in transport in the early embryo and in the dynamic process of dorsal closure. It is believed that its function changes during the life cycle.
(UniProt, Q01989)
Summary (Interactive Fly)

Drosophila myosin VI, a pointed end directed molecular motor - involved in asymmetric cell division - regulates actin dynamics during sperm individualization

Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
4

Please see the GBrowse view of Dmel\jar or the JBrowse view of Dmel\jar for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.47

Gene model reviewed during 6.02

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084636
4316
1253
FBtr0301367
4597
1253
FBtr0301368
4603
1268
FBtr0301369
3587
1073
FBtr0301370
4279
1253
FBtr0301371
4550
1253
FBtr0334871
4361
1268
FBtr0334872
5025
1253
FBtr0345266
4505
1238
Additional Transcript Data and Comments
Reported size (kB)

4.7, 4.5-4.7 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0084020
143.3
1253
8.86
FBpp0290581
143.3
1253
8.86
FBpp0290582
144.9
1268
8.91
FBpp0290583
122.4
1073
8.56
FBpp0290584
143.3
1253
8.86
FBpp0290585
143.3
1253
8.86
FBpp0306894
144.9
1268
8.91
FBpp0306895
143.3
1253
8.86
FBpp0311443
141.8
1238
8.86
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1253 aa isoforms: jar-PB, jar-PG, jar-PJ, jar-PK, jar-PM
1268 aa isoforms: jar-PH, jar-PL
Additional Polypeptide Data and Comments
Reported size (kDa)

1268, 1253, 1078 (aa); 145, 140, 120 (kD)

Comments

The jar protein isoforms differ only at the carboxy-terminal end. All are related to other myosin proteins in the head region. No sequences in the database are related to the tail regions.

The jar protein isoforms differ only at the

carboxy-terminal end. All are related to other myosin proteins in the head

region. No sequences in the database are related to the tail regions.

External Data
Linkouts
Sequences Consistent with the Gene Model
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\jar using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (55 terms)
Molecular Function (11 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253
inferred from direct assay
(assigned by UniProt )
inferred from physical interaction with FLYBASE:mira; FB:FBgn0021776
inferred from physical interaction with UniProtKB:Q9VJE5
(assigned by UniProt )
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
non-traceable author statement
(assigned by UniProt )
inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR004009
(assigned by InterPro )
non-traceable author statement
(assigned by UniProt )
non-traceable author statement
(assigned by UniProt )
inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR004009
(assigned by InterPro )
non-traceable author statement
Biological Process (32 terms)
Terms Based on Experimental Evidence (28 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:l(2)gl; FB:FBgn0002121
inferred from expression pattern
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:shi; FB:FBgn0003392
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
Cellular Component (12 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from physical interaction with FLYBASE:Acam; FB:FBgn0011273
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
non-traceable author statement
(assigned by UniProt )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

jar protein is observed in a punctate pattern throughout the cortical cytoplasm.

jar protein is detected in the border cells in stage 9 developing oocytes. The protein is expressed during border cell migration during the first 6 hours of stage 9. Expression of jar protein is also detected in all the follicle cells starting in mid stage 9 and persisting through stage 10.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687
inferred from physical interaction with FLYBASE:Acam; FB:FBgn0011273
inferred from direct assay
(assigned by UniProt )
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\jar in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 30 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of jar
Transgenic constructs containing regulatory region of jar
Deletions and Duplications ( 9 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
amnioserosa & actin filament
filopodium & border follicle cell, with Scer\GAL4slbo.2.6
follicle cell & nucleus, with Scer\GAL4c532
neuroblast & spindle, with Scer\GAL4VP16.mat.αTub67C
nurse cell & nucleus, with Scer\GAL4c532
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
12 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (3)
11 of 15
Yes
Yes
11 of 15
Yes
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
13 of 15
Yes
Yes
11 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
2 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900V7 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500I5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00W1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00UJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01BJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (9)
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map

    3-84

    Cytogenetic map
    Sequence location
    3R:24,252,826..24,271,233 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    95F6-95F8
    Limits computationally determined from genome sequence between P{PZ}Syx1A06737&P{PZ}Syx1A10660 and P{PZ}crb07207&P{PZ}BRWD305842
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    95F1-95F15
    (determined by in situ hybridisation)
    95F7-95F15
    (determined by in situ hybridisation)
    95F-95F
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (25)
    Genomic Clones (22)
    cDNA Clones (136)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: jar CG5695

    Source for database merge of

    Source for merge of: jar Mhc95F

    Additional comments
    Other Comments

    didum acts to interrupt mitochondrial transport on microtubules to decrease the duty cycle and perhaps facilitate docking in retrograde transport.

    jar is necessary for dorsal closure.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    jar is required for proper localization of mira protein, but not insc protein in embryonic neuroblasts.

    jar is necessary for basal protein targeting and spindle orientation in dividing embryonic neuroblasts.

    dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

    jar is necessary for stabilization of shg and arm proteins in the border follicle cells.

    jar is necessary for border follicle cell migration.

    jar is required for both imaginal disc and egg chamber morphogenesis.

    jar is essential for individualisation of spermatids during spermatogenesis.

    CLIP-190 and jar are coexpressed in a number of tissues during embryogenesis.

    jar protein is involved in intra- and intercellular transport during oogenesis.

    Inhibition of jar function, by injection of anti-jar antibody into embryos, results in profound defects in syncytial blastoderm organisation.

    jar associates with cytoplasmic particles that undergo cell cycle- dependent translocations. In interphase these particles are in cytoplasmic domains surrounding the nuclei near the syncytial blastoderm, during mitosis the particles associate with transient actin rich membrane invaginations that separate neighbouring nuclei. Time lapse computational optical sectioning microscopy has shown directed transport of the particles. The transport is actin based, ATP-dependent and is disrupted by a jar specific antibody.

    Actin based, ATP-dependent linear transport of cytoplasmic particles in syncytial blastoderm embryos can be visualised by antibodies and is catalysed by the unconventional myosin jar.

    Postmeiotic differentiation defect.

    jar encodes a novel class of MHC, first detected as a 140kD actin binding protein by Miller et al., (J. Cell Biol. 109: 2963-2975) by their 3C7 monoclonal antibody. Transcription and splicing patterns are complex, with probably four protein isoforms.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 85 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (34)
    Reported As
    Symbol Synonym
    Dro95F
    ms(3)06746
    myosins VI
    Name Synonyms
    95F Myosin
    95F unconventional myosin
    Myosin heavy chain
    Myosin heavy chain at 95F
    myosin-VI
    unconventional myosin VI
    Secondary FlyBase IDs
    • FBgn0004452
    • FBgn0010074
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (195)