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Gene Dmel\ken

General Information
Symbol	Dmel\ken	Species	D. melanogaster
Name	ken and barbie	Annotation symbol	CG5575
Feature type	protein_coding_gene	FlyBase ID	FBgn0011236
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	ok
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	60A6-60A7	Sequence location	2R:19,757,796..19,764,965 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene ken and barbie is referred to in FlyBase by the symbol Dmel\ken (CG5575, FBgn0011236). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: sequence-specific DNA binding transcription factor activity; nucleic acid binding. There is experimental evidence that it is involved in the biological process: imaginal disc-derived female genitalia development; female analia development; male analia development; insemination; imaginal disc-derived male genitalia development; regulation of JAK-STAT cascade; phagocytosis, engulfment. 32 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; region of integument; integumentary specialisation; rhabdomere R7; larval abdominal segment; spiracle; vagina; late extended germ band embryo. It has one annotated transcript and one annotated polypeptide. Protein features are: BTB/POZ; BTB/POZ fold; BTB/POZ-like; Zinc finger C2H2-type/integrase DNA-binding domain; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-18 embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628840_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ken. Gene sequence location is 2R:19757796..19764965.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60A6-60A7   Limits computationally determined from genome sequence between P{lacW}eIF6k13214 and P{lacW}kenk11035&P{EP}CG17658EP730
Experimentally Determined Cytological Location
Cytogenetic map Notes References 60A-60A     (Kuehnlein, 1998.11.2) 60A5-60A9   (determined by in situ hybridisation)   (Spradling et al., 1999) 60A8-60A11   60A5--9   (Beaton, 1997.12.27) 60A1-60A16   60A5--9 60A8--11   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Yamamoto et al., 1997)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ken for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ken-RA FBtr0072129  NM_079109   3070   601
Additional Transcript Data & Comments
Reported size (kB)	3.2 (northern blot) (Kuhnlein et al., 1998) 3.0 (northern blot) (Lukacsovich et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ken-PA   FBpp0072038   67.0   601   7.07     NP_523833   AAF47084
Additional Polypeptide Data & Comments
Reported size (kDa)	601 (aa) (Lukacsovich et al., 1999, Kuhnlein et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • BTB/POZ-like (IPR000210) Zinc finger, C2H2 (IPR007087) BTB/POZ (IPR013069) BTB/POZ fold (IPR011333) Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087) Zinc finger, C2H2-like (IPR015880)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA694824   AA804103   AA949713   AE013599 AAF47084   AB010260 BAA32683   AB010261 BAA32687   AF181647 AAD55433   AI134915   AI238343   AI293323   AI402290   AJ012576 CAA10062   AQ025948   AQ073275   AY231891   BF505614   BG632938   BG633545   BG641072   BI228777   BI238652   BI365562   BI367645   BI372193   BI483892   CO265446   CO268956   CO274592   CO297714   CO298125   CO300948   CO306134   CO331228   CZ476917   CZ483316   CZ483317   EC061326   EC072088   EC077265   EC085442   EC211257   EC219978   EC245605   EV585605   G01460   GH852283   GH906204   Z50627   Z83443
UniProtKB/Swiss-Prot	O77459
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000436502 2R:19,759,565..19,761,024 evidence=Reporter construct (in vivo) (Jakobsen et al., 2007, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval posterior spiracle   (Kuhnlein et al., 1998) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi embryonic stage 5   organism | 60% egg length   (Kuhnlein et al., 1998) Comment:64% egg length embryonic stage 5 -- 7   organism | 20% egg length   (Kuhnlein et al., 1998) Comment:17% egg length embryonic stage 7 -- 8   dorsal ectoderm   (Fisher et al., 2012) hindgut anlage   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   inclusive hindgut primordium   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic anal pad   (Fisher et al., 2012) embryonic dorsal apodeme   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic ventral apodeme   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval posterior spiracle   (Fisher et al., 2012) embryonic stage 16   embryonic/larval proventriculus   (Kuhnlein et al., 1998) anal pad   (Kuhnlein et al., 1998) dorsal pouch   (Kuhnlein et al., 1998) embryonic/larval foregut   (Kuhnlein et al., 1998) embryonic/larval hindgut   (Kuhnlein et al., 1998) tracheal system   (Kuhnlein et al., 1998) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage   organism   (Lukacsovich et al., 2003) embryonic stage 5   organism | 20% egg length & 70% egg length   (Lukacsovich et al., 2003) embryonic stage 6 -- 7   cephalic furrow | posterior to   (Lukacsovich et al., 2003) embryonic stage 6 -- 15   embryonic hindgut   (Lukacsovich et al., 2003) embryonic stage 15   anal pad   (Lukacsovich et al., 2003) embryonic foregut   (Lukacsovich et al., 2003) third instar larval stage   genital disc   (Lukacsovich et al., 2003) eye-antennal disc   (Lukacsovich et al., 2003) adult stage   organism   (Lukacsovich et al., 2003)
Additional Descriptive Data
	ken transcripts are first detected in early stage 5 embryos in two circumferential rings around the embryo at 17% and 64% egg length. At the end of stage 5, they co-localize with the first and seventh ftz stripes. The anterior stripe fades while the staining in the posterior domain persists until the end of embyrogenesis in the posterior spiracles. Expression is also observed at stage 16 in the foregut, the proventriculus, the dorsal pouch, the hindgut, and the anal pads. (Kuhnlein et al., 1998) Northern analysis indicates that expression of ken is higher during embryonic and pupal stages than in larval stages. Expression of ken is higher in adult females than in males. ken transcript is expressed ubiquitously in both male and female genital discs and eye-antennal discs. In the embryo ken transcript is detected in stage 5 blastoderm embryos as 2 stripes at 64% and 17% egg length. Expression is stronger with the anterior expression just posterior to the cephalic furrow and posterior expression confined to the hindgut primordium. Anterior expression disappears by stage 9 and then reappears in the foregut at stage 15. Posterior expression is confined to the hindgut and anal pad. (Lukacsovich et al., 2003)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
Reporter: P{PZ}ken1 Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage   embryonic/larval trachea   (Meister and Braun, 1995.10) imaginal disc   (Meister and Braun, 1995.10) embryonic/larval brain   (Meister and Braun, 1995.10) ring gland   (Meister and Braun, 1995.10) embryonic/larval hindgut   (Meister and Braun, 1995.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    99.35 Larval Midgut    40 Larval Hindgut    79.8 Larval Malpighian Tubules    15.5 Larval Fat Body    23 Larval Salivary Gland    119.7 Larval Trachea    204.75 Larval Carcass    151.775 Adult Head    97.1 Adult Eye    95.025 Adult Brain    41.6 Adult Thoracic-Abdominal Ganglion    49.8 Adult Crop    137.7 Adult Midgut    60.3 Adult Hindgut    108.2 Adult Malpighian Tubules    116.5 Adult Fat Body    83.3 Adult Salivary Gland    188.4 Adult Heart    81.625 Adult VirginFemale Spermatheca    111.9 Adult InseminatedFemale Spermatheca    125.5 Adult Ovary    405.5 Adult Testis    97.7 Adult Male Accessory Gland    82.4 Adult Carcass    101.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    97.1   NA  Eye    95.025   NA  Brain    41.6   99.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    49.8   NA  Crop    137.7   40  Midgut    60.3   79.8  Hindgut    108.2   15.5  Malpighian Tubules    116.5   no informative data  Fat Body    83.3   119.7  Salivary Gland    188.4   NA  Heart    81.625   204.75  Trachea    NA   NA  VirginFemale Spermatheca    111.9   NA  InseminatedFemale Spermatheca    125.5   NA  Ovary    405.5   NA  Testis    97.7   NA  Male Accessory Gland    82.4   151.775  Carcass    101.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    44 central nervous system, larvae L3    13 central nervous system, pupae P8    29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    39 carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female    54 ovary, mated 4-day female    102 testis, mated 4-day male    31 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (44) central nervous system, larvae L3    13 central nervous system, pupae P8  (29) head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8  (39) carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female  (54) ovary, mated 4-day female  (102) testis, mated 4-day male  (31) accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    44 central nervous system, larvae L3    13 central nervous system, pupae P8    29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    39 carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female    54 ovary, mated 4-day female  102 testis, mated 4-day male    31 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    44 central nervous system, larvae L3    13 central nervous system, pupae P8    29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    39 carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female    54 ovary, mated 4-day female    102 testis, mated 4-day male    31 accessory gland, mated 4-day male    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    44 central nervous system, larvae L3    13 central nervous system, pupae P8    29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    39 carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female    54 ovary, mated 4-day female    102 testis, mated 4-day male    31 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (44) central nervous system, larvae L3    13 central nervous system, pupae P8  29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8  (39) carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female  (54) ovary, mated 4-day female  (102) testis, mated 4-day male  31 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    44 central nervous system, larvae L3    13 central nervous system, pupae P8    29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    39 carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female    54 ovary, mated 4-day female  102 testis, mated 4-day male    31 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    44 central nervous system, larvae L3    13 central nervous system, pupae P8    29 head, virgin 1-day female    19 head, virgin 4-day female    15 head, virgin 20-day female    11 head, mated 1-day female    18 head, mated 4-day female    10 head, mated 20-day female    8 head, mated 1-day male    21 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    39 carcass, larvae L3 wandering    15 carcass, 1-day adult    14 carcass, 4-day adult    14 carcass, 20-day adult    17 ovary, virgin 4-day female    54 ovary, mated 4-day female    102 testis, mated 4-day male    31 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-18 embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr    29 embryo 14-16hr    145 embryo 16-18hr    141 embryo 18-20hr    66 embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2    34 larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    28 pupae 4d postWPP    29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr  (29) embryo 14-16hr  (145) embryo 16-18hr  (141) embryo 18-20hr  (66) embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2  (34) larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP  (47) pupae 3d postWPP  (28) pupae 4d postWPP  (29) adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day  (27) adult female 30day  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr    29 embryo 14-16hr  (145) embryo 16-18hr  (141) embryo 18-20hr    66 embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2    34 larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    28 pupae 4d postWPP    29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr    29 embryo 14-16hr    145 embryo 16-18hr    141 embryo 18-20hr    66 embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2    34 larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    28 pupae 4d postWPP    29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day    27 adult female 30day    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr    29 embryo 14-16hr    145 embryo 16-18hr    141 embryo 18-20hr    66 embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2    34 larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    28 pupae 4d postWPP    29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr  29 embryo 14-16hr  (145) embryo 16-18hr  (141) embryo 18-20hr  (66) embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2  (34) larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP  (47) pupae 3d postWPP  28 pupae 4d postWPP  29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day  27 adult female 30day  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr    29 embryo 14-16hr  (145) embryo 16-18hr  141 embryo 18-20hr    66 embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2    34 larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    28 pupae 4d postWPP    29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    10 embryo 04-06hr    7 embryo 06-08hr    2 embryo 08-10hr    5 embryo 10-12hr    15 embryo 12-14hr    29 embryo 14-16hr    145 embryo 16-18hr    141 embryo 18-20hr    66 embryo 20-22hr    13 embryo 22-24hr    14 larva L1    12 larva L2    34 larva L3 12hr old    12 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    25 white prepupae 24hr    18 pupae 2d postWPP    47 pupae 3d postWPP    28 pupae 4d postWPP    29 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    21 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    45 Schneider line 2 Sg4    30 embryonic 1182-4H    56 embryonic GM2    42 embryonic Kc167    51 embryonic S1    106 embryonic S3    26 leg disc CME L1    52 wing disc CME-W2    25 wing disc ML-DmD8    35 wing disc ML-DmD9    75 wing disc ML-DmD16-c3    104 wing disc ML-DmD21    32 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    56 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    44 ovary fGS/OSS    236 ovary OSC    93 ovary OSS    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (45) Schneider line 2 Sg4  (30) embryonic 1182-4H  (56) embryonic GM2  (42) embryonic Kc167  (51) embryonic S1  (106) embryonic S3  26 leg disc CME L1  (52) wing disc CME-W2    25 wing disc ML-DmD8  (35) wing disc ML-DmD9  (75) wing disc ML-DmD16-c3  (104) wing disc ML-DmD21  (32) wing disc ML-DmD32  (53) haltere disc ML-DmD17-c3  (56) eye-antennal disc ML-DmD11  (58) antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1  (40) CNS ML-DmBG1-c1  (47) CNS ML-DmBG2-c2  26 tumorous blood cells mbn2  (44) ovary fGS/OSS  (236) ovary OSC  (93) ovary OSS  (55) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    45 Schneider line 2 Sg4    30 embryonic 1182-4H    56 embryonic GM2    42 embryonic Kc167    51 embryonic S1  106 embryonic S3    26 leg disc CME L1    52 wing disc CME-W2    25 wing disc ML-DmD8    35 wing disc ML-DmD9    75 wing disc ML-DmD16-c3  104 wing disc ML-DmD21    32 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    56 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    44 ovary fGS/OSS  (236) ovary OSC    93 ovary OSS    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    45 Schneider line 2 Sg4    30 embryonic 1182-4H    56 embryonic GM2    42 embryonic Kc167    51 embryonic S1    106 embryonic S3    26 leg disc CME L1    52 wing disc CME-W2    25 wing disc ML-DmD8    35 wing disc ML-DmD9    75 wing disc ML-DmD16-c3    104 wing disc ML-DmD21    32 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    56 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    44 ovary fGS/OSS    236 ovary OSC    93 ovary OSS    55 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    45 Schneider line 2 Sg4    30 embryonic 1182-4H    56 embryonic GM2    42 embryonic Kc167    51 embryonic S1    106 embryonic S3    26 leg disc CME L1    52 wing disc CME-W2    25 wing disc ML-DmD8    35 wing disc ML-DmD9    75 wing disc ML-DmD16-c3    104 wing disc ML-DmD21    32 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    56 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    44 ovary fGS/OSS    236 ovary OSC    93 ovary OSS    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (45) Schneider line 2 Sg4  30 embryonic 1182-4H  (56) embryonic GM2  (42) embryonic Kc167  (51) embryonic S1  (106) embryonic S3  26 leg disc CME L1  (52) wing disc CME-W2    25 wing disc ML-DmD8  (35) wing disc ML-DmD9  (75) wing disc ML-DmD16-c3  (104) wing disc ML-DmD21  32 wing disc ML-DmD32  (53) haltere disc ML-DmD17-c3  (56) eye-antennal disc ML-DmD11  (58) antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1  (40) CNS ML-DmBG1-c1  (47) CNS ML-DmBG2-c2  26 tumorous blood cells mbn2  (44) ovary fGS/OSS  (236) ovary OSC  (93) ovary OSS  (55) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    45 Schneider line 2 Sg4    30 embryonic 1182-4H    56 embryonic GM2    42 embryonic Kc167    51 embryonic S1  106 embryonic S3    26 leg disc CME L1    52 wing disc CME-W2    25 wing disc ML-DmD8    35 wing disc ML-DmD9    75 wing disc ML-DmD16-c3  104 wing disc ML-DmD21    32 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    56 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    44 ovary fGS/OSS  (236) ovary OSC    93 ovary OSS    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    45 Schneider line 2 Sg4    30 embryonic 1182-4H    56 embryonic GM2    42 embryonic Kc167    51 embryonic S1    106 embryonic S3    26 leg disc CME L1    52 wing disc CME-W2    25 wing disc ML-DmD8    35 wing disc ML-DmD9    75 wing disc ML-DmD16-c3    104 wing disc ML-DmD21    32 wing disc ML-DmD32    53 haltere disc ML-DmD17-c3    56 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    47 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    44 ovary fGS/OSS    236 ovary OSC    93 ovary OSS    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    64 cold shock, 4-day adult    37 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    86 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    43 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    48 Paraquat 5 mM 48 hrs, 4-day adult    66 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (64) cold shock, 4-day adult  (37) heat shock, 4-day adult  (32) Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult  (40) Cadmium 100 mM 48 hrs, 4-day adult  (86) Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult  (43) Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (63) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (68) Caffeine 25 mg/ml 48 hrs, 4-day adult  (48) Paraquat 5 mM 48 hrs, 4-day adult  (66) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    64 cold shock, 4-day adult    37 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    86 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    43 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    48 Paraquat 5 mM 48 hrs, 4-day adult    66 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    64 cold shock, 4-day adult    37 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    86 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    43 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    48 Paraquat 5 mM 48 hrs, 4-day adult    66 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    64 cold shock, 4-day adult    37 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    86 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    43 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    48 Paraquat 5 mM 48 hrs, 4-day adult    66 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (64) cold shock, 4-day adult  (37) heat shock, 4-day adult  32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult  (40) Cadmium 100 mM 48 hrs, 4-day adult  (86) Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult  (43) Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (63) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (68) Caffeine 25 mg/ml 48 hrs, 4-day adult  (48) Paraquat 5 mM 48 hrs, 4-day adult  (66) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    64 cold shock, 4-day adult    37 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    86 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    43 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    48 Paraquat 5 mM 48 hrs, 4-day adult    66 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    64 cold shock, 4-day adult    37 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    86 Copper 0.5 mM 12 hrs, larvae L3    23 Copper 15 mM 48 hrs, 4-day adult    43 Zinc 5 mM 12 hrs, larvae L3    24 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    48 Paraquat 5 mM 48 hrs, 4-day adult    66 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • GH12495 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011236 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5575-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for ken Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal kenk11035 lethal, with Scer\GAL4pnr-MD237 kenGD16293 lethal | dominant kenk11035 lethal | pupal stage ken1 lethal | pupal stage (with Df(2R)Chig230) ken1 ken02970 lethal | recessive ken00628 ken02970 ken03907 ken05029 ken08253 kenk08507b kenk11035 partially lethal - majority die ken1 ken02970 Other Phenotypes Allele visible ken1 kenok visible | somatic clone kenunspecified Phenotype manifest in Allele antenna ken1 antenna (with Df(2R)Chig230) ken02970 arista ken1 kenok clasper ken1 embryonic/larval posterior spiracle (with Df(2R)Chig230), with Df(1)os1A2 kenk11035 female analia ken1 female genitalia ken1 female gonopod ken1 genitalia ken1 genitalia (with Df(2R)Chig230) ken02970 male genital disc (with ken1) kenok male genital disc (with kenok) ken1 male genitalia ken1 kenok penis ken1 rhabdomere R7, with Scer\GAL4Rh3.PP kenGS11590 terminalia (with ken1) ken02970 terminalia (with ken00628) kenok terminalia (with ken02970) ken1 kenok terminalia (with kenok) ken00628 ken02970 tracheal pit, with Scer\GAL4da.G32 kenScer\UAS.cAa vulva ken1 wing margin bristle | somatic clone kenunspecified
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of ken Class Mutagen Stocks Known lesion kenok P-element activity 3 Yes ken00628 P-element activity 1 Yes ken02970 hypomorphic allele - genetic evidence P-element activity 1 Yes ken1 hypomorphic allele - genetic evidence P-element activity 1 Yes kend03038 1 -- kenf00833 piggyBac transposase 1 -- kenf04247 1 -- kenGS11590 1 Yes kenk11035 P-element activity 1 Yes kenNP3604 1 -- kenNP4279 1 -- kenNP6255 1 -- kenUM-8215-3 1 -- ken03907 P-element activity 0 Yes ken05029 P-element activity 0 -- ken08253 P-element activity 0 -- ken5-SZ-3133 0 -- kenBG01259 P-element activity 0 -- kenCB-5269-3 0 -- kend09748 P-element activity 0 -- kenk08507b P-element activity 0 -- kenok-Rev1 Delta2-3 0 Yes kenunspecified 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ken Class Mutagen Stocks Known lesion kenGD16293 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes kenHMS01219 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ken+t9 in vitro construct - genomic fragment 0 Yes kenGD4365 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes kenhs.PL in vitro construct - regulatory fusion 0 Yes kenScer\UAS.cAa in vitro construct - regulatory fusion 0 Yes kenScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes kenScer\UAS.cLa in vitro construct - regulatory fusion 0 Yes kenScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC610 (Christensen et al., 2008.9.3) Df(2R)BSC660 (Christensen et al., 2008.12.28) Df(2R)bwVDe2LPxKR (Spradling et al., 1999, BDGP Project Members, 1994-1999, Beaton, 1997.12.27) Df(2R)bw-S46 (Spradling et al., 1999, Castrillon et al., 1993, BDGP Project Members, 1994-1999) Df(2R)or-BR11 (Spradling et al., 1999, BDGP Project Members, 1994-1999) In(2LR)ltG10LBR27R (BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)en-SFX31 (BDGP Project Members, 1994-1999) In(2LR)Px4 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4365} NA P{GD16293} NA P{TRiP.HMS01219} NA P{UAS-ken.A} NA P{UAS-ken.B} NA P{UAS-ken.GFP} NA P{UAS-ken.L} NA heat-shock construct P{hs-ken.L} NA characterization construct P{ken+t9} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}kenk08507b No P{lacW}kenk11035 No P{lacW}l(2)SH0321SH0321 No P{lacW}Thiolasek09828 No P{PZ}ken1 No P{PZ}ken00628 No P{PZ}ken02970 No P{PZ}ken03907 No P{PZ}ken05029 No P{PZ}ken08253 No miscellaneous insertions P{}kenok NA P{GT1}kenBG01259 NA P{RS3}kenCB-5269-3 NA P{RS3}kenUM-8215-3 NA P{RS5}ken5-SZ-3133 NA PBac{WH}kenf00833 NA PBac{WH}kenf04247 NA insertion of enhancer trap binary system P{GawB}kenNP3604 No P{GawB}kenNP4279 No P{GawB}kenNP6255 No insertion of mobile activating element P{GSV1}GSd220GSd220 NA P{GSV1}GSd482GSd482 NA P{GSV6}kenGS11590 NA P{XP}kend03038 NA P{XP}kend09748 NA
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
female analia development inferred from mutant phenotype (assigned by UniProtKB) (Lukacsovich et al., 2003) imaginal disc-derived female genitalia development inferred from mutant phenotype (assigned by UniProtKB) (Lukacsovich et al., 2003) imaginal disc-derived male genitalia development inferred from mutant phenotype (assigned by UniProtKB) (Lukacsovich et al., 2003) insemination inferred from mutant phenotype (Castrillon et al., 1993) male analia development inferred from mutant phenotype (assigned by UniProtKB) (Lukacsovich et al., 2003) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) regulation of JAK-STAT cascade inferred from mutant phenotype (Arbouzova et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
nucleic acid binding inferred from sequence or structural similarity with InterPro:IPR007087 (FlyBase, 1992-) sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity (assigned by UniProtKB) (Lukacsovich et al., 2003) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
copulation non-traceable author statement (Billeter et al., 2002)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with InterPro:IPR007087 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
ken - CG7518 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ken - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ken - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ken - lolal anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) ken - pzg anti bait coimmunoprecipitation, western blot (Kugler et al., 2011) ken - skpA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ken allele Gene References kenok cad (Lukacsovich et al., 2003) dpp (Lukacsovich et al., 2003) tll (Lukacsovich et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63378 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011236 DroID - A comprehensive database of gene and protein interactions. • FBgn0011236 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37785
Orthologs
OrthoDB Orthologs (29) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6S1TT0 Dipteran inclusive ortholog search EOG6TTGSH Insect inclusive ortholog search EOG6HX3WN Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6S1TT0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ken     Drosophila simulans   Dsim\GD13771 Y   Drosophila sechellia   Dsec\GM16054 Y   Drosophila erecta   Dere\GG22895 Y   Drosophila yakuba   Dyak\ken Y   Drosophila ananassae   Dana\GF12868 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18980 Y   Drosophila persimilis   Dper\GL11171 Y   Drosophila willistoni   Dwil\GK15862 Y   Drosophila virilis   Dvir\GJ21870 Y   Drosophila mojavensis   Dmoj\GI18841 Y   Drosophila grimshawi   Dgri\GH21687 Y
Orthologs in non-Drosophila Dipterans (EOG6TTGSH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002474   Anopheles gambiae Malaria mosquito  Agam\AGAP004767   Culex quinquefasciatus Southern house mosquito  Cqui\ken1   Culex quinquefasciatus Southern house mosquito  Cqui\ken2
Orthologs in non-Dipteran Insects (EOG6HX3WN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\ken   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007578   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16756   Atta cephalotes Leafcutter ant  Acep\ACEP11998   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18791   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16226   Linepithema humile Argentine ant  Lhum\LH23209   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21153   Solenopsis invicta Red fire ant  Sinv\SINV11409   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005188   Bombyx mori Silkmoth  Bmor\BGIBMGA002702   Pediculus humanus Human body louse  Phum\PHUM427690   Tribolium castaneum Red flour beetle  Tcas\TC001442
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13771 Drosophila sechellia Dsec\GM16054 Drosophila erecta Dere\GG22895 Drosophila yakuba Dyak\ken Drosophila ananassae Dana\GF12868 Drosophila pseudoobscura pseudoobscura Dpse\GA18980 Drosophila persimilis Dper\GL11171 Drosophila willistoni Dwil\GK15862 Drosophila virilis Dvir\GJ21870 Drosophila mojavensis Dmoj\GI18841 Drosophila grimshawi Dgri\GH21687
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	11753 cn1 P{PZ}ken1/CyO; ry506 11244 cn1 P{PZ}ken02970/CyO; ry506 34739 y1 sc* v1; P{TRiP.HMS01219}attP2
Harvard	- P{XP}kend03038 - PBac{WH}kenf00833 - PBac{WH}kenf04247
Kyoto	124419 w1118; P{RS3}kenUM-8215-3 109040 w1118; P{BmDelta-w}kenok / SM1 205322 y1 w67c23; P{GSV6}kenGS11590/SM1 103371 y1 w67c23 ; P{lacW}kenk11035 / CyO 109045 w1118; P{BmDelta-w}kenok P{hs-ken.L}9-2 / SM1
VDRC	v48596 w1118; P{GD16293}v48596
	More stocks available...
Genomic Clones ( 1 )
	BACR05F24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH12495
Other clones	DPiM_GH12495 yak-em_ken
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT07874 GH14371 GM01621 GM12839 LD29702 RE26421 RE35158 RE50920 RE53448 RE59086 RE66854 SD12314
Other clones	13015 32055 50336 53531 54672 144313 CK01.121D.H12 Dmel_dig_001174_0710_3723 EK093162 EK114549 EK142664 EK170409 EK171540 EK179745 EK250330 EK298848 RP003020089 RP003102914
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011236 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37785
Antibody Information
	polyclonal (Issigonis and Matunis, 2012)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ken CG5575 (Zhang, 1999.11)
Source for database merge of	Source for merge of: ken okina (Lukacsovich et al., 1999)
Additional comments
Other Comments
	S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) Mutants show malformation of adult terminalia. (Lukacsovich et al., 2003) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) Mutant homozygous males prematurely terminate copulation. (Yamamoto et al., 1997) Sperm transfer defect. (Castrillon et al., 1993) Locus originally described and named by C. Berg.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079109 RefSeq (Proteins) • NP_523833 DDBJ / EMBL / GenBank • AA694824 AA804103 AA949713 AAD55433 AAF47084 AB010260 AB010261 AF181647 AI134915 AI293323 AI402290 AJ012576 AQ025948 AQ073275 BAA32683 BAA32687 BG632938 BG633545 CAA10062 CZ476917 CZ483316 CZ483317 G01460 Z50627 Z83443 Entrez Gene - A searchable database of RefSeq genes. • 37785 UniProtKB/Swiss-Prot • O77459
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • BTB/POZ-like (IPR000210) Zinc finger, C2H2 (IPR007087) BTB/POZ (IPR013069) BTB/POZ fold (IPR011333) Zinc finger C2H2-type/integrase DNA-binding domain (IPR013087) Zinc finger, C2H2-like (IPR015880) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH12495
Linkouts
	BioGRID - A database of protein and genetic interactions • 63378 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011236 DroID - A comprehensive database of gene and protein interactions. • FBgn0011236 DRSC - Results from RNAi screens. • FBgn0011236 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011236 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5575-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011236 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 149310 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37785 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/ken&barbie.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37785 modMine - Data generated by the modENCODE project. • FBgn0011236
Synonyms & Secondary IDs ( 21 )
Reported As
Symbol Synonym	2970 (Harvie et al., 1998) 3907 (Harvie et al., 1998) 5029 (Harvie et al., 1998) 8253 (Harvie et al., 1998) BCL-6/KEN (Castelli-Gair Hombria, 2006) CG5575 (Arbouzova et al., 2006, Quinones-Coello, 2007, Brandt, 2006, Haussmann et al., 2008, Ni et al., 2010.12.1, Kanuka et al., 2005) ken (Arbouzova, 2006, Bullock et al., 2006, Castelli-Gair Hombria, 2006, Christensen et al., 2008.9.3, Stroschein-Stevenson et al., 2006, Quinones-Coello, 2007, Maeda et al., 2008, Baeg et al., 2007, McClure and Schubiger, 2008, Minidorff et al., 2007, Grimberg et al., 2011, Minidorff et al., 2007, Yoon et al., 2011, Levis et al., 2011.12.16, Christensen et al., 2008.12.28, Weake et al., 2011, Flaherty et al., 2009, Brandt, 2006, Haussmann et al., 2008, Berger et al., 2012, Kanuka et al., 2005) Ken (Arbouzova, 2006, Arbouzova et al., 2006, Bonchuk et al., 2011, Kugler et al., 2011, Franchini et al., 2004) KEN (Castelli-Gair Hombria, 2006, Arbouzova and Zeidler, 2006) l(2)00628 (Liebl, 1999) l(2)02970   l(2)05029   ok (Billeter et al., 2002, Yamamoto and Nakano, 1998) okina (Yamamoto et al., 1997, ) P420 (Liebl, 1999) unnamed (Kolhekar et al., 1997)
Name Synonym	ken & barbie (Arbouzova et al., 2003, Arbouzova et al., 2006) Ken & Barbie (Arbouzova, 2006, Arbouzova et al., 2006, Arbouzova and Zeidler, 2006, Maeng et al., 2012) ken and barbie (McClure and Schubiger, 2008, Flaherty et al., 2009, Jordan et al., 2007) ken-and-barbie (Kula-Eversole and Hatini, 2007, Wang et al., 2006) Ken and Barbie (Castelli-Gair Hombria, 2006, Yoon et al., 2011)
Secondary FlyBase IDs
	FBgn0010464 FBgn0010525 FBgn0020522 FBgn0024882
References ( 83 )
Generate a list of	All references relating to this gene ( 83 )
List References by type	Research paper  ( 35 ) Supplementary material  ( 12 ) Review  ( 6 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Curated genome annotation  ( 3 ) Website  ( 1 )
Recent research papers ( 9 )
	Berger et al., 2012, Cell Rep. 2(2): 407--418 FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. [FBrf0219320] Issigonis and Matunis, 2012, Dev. Biol. 368(2): 181--192 The Drosophila BCL6 homolog ken and barbie promotes somatic stem cell self-renewal in the testis niche. [FBrf0218994] Maeng et al., 2012, Mol. Cells 33(5): 457--463 The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development. [FBrf0218378] Bonchuk et al., 2011, J. Mol. Biol. 412(3): 423--436 Drosophila BTB/POZ Domains of "ttk Group" Can Form Multimers and Selectively Interact with Each Other. [FBrf0215054] Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Grimberg et al., 2011, Mol. Cell. Biol. 31(4): 897--909 Basic Leucine Zipper Protein Cnc-C Is a Substrate and Transcriptional Regulator of the Drosophila 26S Proteasome. [FBrf0212906] Kugler et al., 2011, Genetics 188(1): 127--139 The Putzig-NURF Nucleosome Remodeling Complex Is Required for Ecdysone Receptor Signaling and Innate Immunity in Drosophila melanogaster. [FBrf0213648] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Yoon et al., 2011, Nat. Cell Biol. 13(9): 1062--1069 miRNA-mediated feedback inhibition of JAK/STAT morphogen signalling establishes a cell fate threshold. [FBrf0215016] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...