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Gene Dmel\Sema-2a

General Information
Symbol	Dmel\Sema-2a	Species	D. melanogaster
Name	Sema-2a	Annotation symbol	CG4700
Feature type	protein_coding_gene	FlyBase ID	FBgn0011260
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	Sema2a, SemaII, D-SemaII, Dsema-II
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	53C6-53C7	Sequence location	2R:12,387,683..12,420,964 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Sema-2a is referred to in FlyBase by the symbol Dmel\Sema-2a (CG4700, FBgn0011260). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: adult behavior; axon guidance; visual behavior; flight behavior; synaptic target inhibition; salivary gland development; drinking behavior; dendrite guidance. 25 alleles are reported. The phenotypes of these alleles are annotated with: organ system; embryonic/larval neuron; multicellular structure; organ system subdivision; larval abdominal segment; peripheral nervous system; anatomical structure; synapse; imaginal precursor; embryonic/larval imaginal precursor. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Immunoglobulin subtype; Immunoglobulin-like domain; Immunoglobulin-like fold; Plexin-like fold; Plexin/semaphorin/integrin; Semaphorin; Semaphorin/CD100 antigen; WD40/YVTN repeat-like-containing domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult heart, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult midgut, larval hindgut, adult fat body, larval salivary gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1629819_s_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Sema-2a. Gene sequence location is 2R:12387683..12420964.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 53C6-53C7   Limits computationally determined from genome sequence between P{lacW}l(2)k07824k07824&P{lacW}vegk03402 and P{PZ}Sema-2a03021&P{PZ}Cdk405428
Experimentally Determined Cytological Location
Cytogenetic map Notes References 53C6-53C10   (determined by in situ hybridisation)   (Spradling et al., 1999) 53C3-53C10     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Sema-2a for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Sema-2a-RA FBtr0087090  NM_058159   3580   724 Sema-2a-RB FBtr0087089  NM_134303   3481   724 Sema-2a-RC FBtr0087088  NM_166178   3548   697
Additional Transcript Data & Comments
Reported size (kB)	4.0 (northern blot) (Kolodkin et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Sema-2a-PA   FBpp0086238   83.0   724   7.98     NP_477507   AAF57989 Sema-2a-PB   FBpp0086237   83.0   724   7.98     NP_599130   AAF57990 Sema-2a-PC   FBpp0086236   80.0   697   8.02     NP_725586   AAM68500
Additional Polypeptide Data & Comments
Reported size (kDa)	707 (aa) (Kolodkin et al., 1993)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WD40/YVTN repeat-like-containing domain (IPR015943) Semaphorin/CD100 antigen (IPR001627) Immunoglobulin subtype (IPR003599) Immunoglobulin-like domain (IPR007110) Plexin/semaphorin/integrin (IPR003659) Plexin-like fold (IPR016201) Semaphorin (IPR027231) Immunoglobulin-like fold (IPR013783)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141641   AA201106   AA201433   AA202026   AA246757   AA263826   AA264846   AA390412   AA391479   AA392296   AA392807   AA538907   AE013599 AAF57989   AE013599 AAF57990   AE013599 AAM68500   AC004432   AC005147   AC005647   AI515505   AQ073276   AW942069   AW942079   AY095026 AAM11354   AY128437 AAM75030   BG639224   BI162559   BI165127   BI168253   BI171815   BI172622   BI212353   BI215897   BI216167   BI229818   BI230837   BI233257   BI234629   BI235976   BI237666   BI239774   BI240663   BI241129   BI241884   BI243686   BI358423   BI362027   BI367713   BI374450   BI483089   BI483523   BI484006   BI484386   BI484450   BI486467   BI570372   BI577529   BI577825   BI580262   BI580895   BI613730   BP556937   BP560064   BT011447   BT071804   CK658115   CL525990   CO177071   CO177703   CO179494   CO187281   CO187562   CO263164   CO269516   CO273932   CO285597   CO291269   CO291994   CO297211   CO298360   CO298369   CO304781   CO307786   CO308368   CO308481   CO316346   CO322194   CO324215   CO324684   CO331422   CO332739   CZ482468   CZ489243   CZ489244   DN154338   EC066262   EC067656   EC080283   EC198074   EC214471   EC216628   EC217953   EC219582   EC224191   EC226501   EC226715   EC231228   EC236502   EC245976   EC246974   EC249550   EV577204   GH791320   GH818609   GH825290   GH836584   GH843757   GH905254   GH911189   GH916802   GH919959   GH935084   GH941830   GH945169   GH947080   GH948825   GH949674   GH949694   GH950337   GH951207   GH953109   GH955722   GH955819   GH956485   GH957861   GH960872   GH961633   GH961908   GH961924   GH963573   GH964062   GH964272   GH965580   GH965681   GH967851   GH968600   GH968896   GH969011   GH970200   GH971766   GH973559   GH974063   GH974990   GH978620   GH982858   GH982900   GH983820   GH983938   GH984850   L26083 AAC37187
UniProtKB/Swiss-Prot	Q24323
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   sense organ | anterior   (Kolodkin et al., 1993) embryonic/larval neuron   (Kolodkin et al., 1993) embryonic stage 14 -- 17   hypodermal muscle of metathorax | ventral   (Kolodkin et al., 1993) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   gonad   (Kolodkin et al., 1993) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   dorsal ectoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 10   epidermis | striped   (Kolodkin et al., 1993) embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 15 -- 17   central nervous system | restricted   (Kolodkin et al., 1993)
Additional Descriptive Data
	Sema-2a expression is first detected at embryonic stage 10 in weak epidermal stripes. It is also found in the CNS starting in stage 15 in a restricted set of neurons. Beginning in stage 14, Sema-2a is expressed in a single ventral thoracic muscle fiber in the metathoracic segment. Expression occurs also in embryonic gonads and anterior sense organs. (Kolodkin et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference prepupal stage P1   antennal lobe | restricted   (Sweeney et al., 2011) Comment:0h APF prepupal stage P3   antennal lobe | restricted   (Sweeney et al., 2011) Comment:6h APF prepupal stage P4(ii)   antennal lobe | restricted   (Sweeney et al., 2011) Comment:12h APF pupal stage P5(ii)   antennal lobe | restricted   (Sweeney et al., 2011) Comment:16h APF
Additional Descriptive Data
	Sema-2a protein is expressed in the medial and ventromedial antennal lobe. This pattern is similar to the one of Sema-2b protein, except that the gradient of Sema-2a protein is steeper than the one of Sema-2b. The level of expression of Sema-2a is opposite to the one of Sema-1a: if the expression level of Sema-2a is high, the one of Sema-1a is low, and vice-versa. (Sweeney et al., 2011)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult heart, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult midgut, larval hindgut, adult fat body, larval salivary gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1045.8 Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea    729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart    817.925 Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary    701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1045.8) Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea  (729.5) Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart  (817.925) Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary  (701.1) Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1045.8 Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea    729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart    817.925 Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary    701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1045.8 Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea    729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart    817.925 Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary    701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1045.8 Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea    729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart    817.925 Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary    701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1045.8) Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea  729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart  (817.925) Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary  701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1045.8 Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea    729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart    817.925 Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary    701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1045.8 Larval Midgut    90.7 Larval Hindgut    102.4 Larval Malpighian Tubules    69 Larval Fat Body    79.1 Larval Salivary Gland    136.8 Larval Trachea    729.5 Larval Carcass    216.525 Adult Head    242.4 Adult Eye    93.4 Adult Brain    474.7 Adult Thoracic-Abdominal Ganglion    293.6 Adult Crop    89.1 Adult Midgut    140.6 Adult Hindgut    61 Adult Malpighian Tubules    21.7 Adult Fat Body    151.1 Adult Salivary Gland    15.1 Adult Heart    817.925 Adult VirginFemale Spermatheca    32.2 Adult InseminatedFemale Spermatheca    39.3 Adult Ovary    701.1 Adult Testis    53.1 Adult Male Accessory Gland    54.5 Adult Carcass    176 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    242.4   NA  Eye    93.4   NA  Brain    474.7   1045.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    293.6   NA  Crop    89.1   90.7  Midgut    140.6   102.4  Hindgut    61   69  Malpighian Tubules    no informative data   79.1  Fat Body    151.1   136.8  Salivary Gland    no informative data   NA  Heart    817.925   729.5  Trachea    NA   NA  VirginFemale Spermatheca    32.2   NA  InseminatedFemale Spermatheca    39.3   NA  Ovary    701.1   NA  Testis    53.1   NA  Male Accessory Gland    54.5   216.525  Carcass    176 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    84 central nervous system, larvae L3    70 central nervous system, pupae P8    103 head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8    28 carcass, larvae L3 wandering    68 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female    67 ovary, mated 4-day female    56 testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (84) central nervous system, larvae L3  (70) central nervous system, pupae P8  (103) head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8  (28) carcass, larvae L3 wandering  (68) carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female  (67) ovary, mated 4-day female  (56) testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    84 central nervous system, larvae L3    70 central nervous system, pupae P8  103 head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8    28 carcass, larvae L3 wandering    68 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female    67 ovary, mated 4-day female    56 testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    84 central nervous system, larvae L3    70 central nervous system, pupae P8    103 head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8    28 carcass, larvae L3 wandering    68 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female    67 ovary, mated 4-day female    56 testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    84 central nervous system, larvae L3    70 central nervous system, pupae P8    103 head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8    28 carcass, larvae L3 wandering    68 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female    67 ovary, mated 4-day female    56 testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (84) central nervous system, larvae L3  (70) central nervous system, pupae P8  (103) head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8  28 carcass, larvae L3 wandering  (68) carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female  (67) ovary, mated 4-day female  (56) testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    84 central nervous system, larvae L3    70 central nervous system, pupae P8  103 head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8    28 carcass, larvae L3 wandering    68 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female    67 ovary, mated 4-day female    56 testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    84 central nervous system, larvae L3    70 central nervous system, pupae P8    103 head, virgin 1-day female    17 head, virgin 4-day female    13 head, virgin 20-day female    8 head, mated 1-day female    10 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    14 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    18 digestive system, larvae L3 wandering    17 digestive system, 1-day adult    3 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    1 fat body, white prepupae    19 fat body, pupae P8    28 carcass, larvae L3 wandering    68 carcass, 1-day adult    7 carcass, 4-day adult    9 carcass, 20-day adult    5 ovary, virgin 4-day female    67 ovary, mated 4-day female    56 testis, mated 4-day male    3 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    105 embryo 04-06hr    164 embryo 06-08hr    165 embryo 08-10hr    173 embryo 10-12hr    113 embryo 12-14hr    133 embryo 14-16hr    77 embryo 16-18hr    59 embryo 18-20hr    32 embryo 20-22hr    44 embryo 22-24hr    48 larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    49 white prepupae new    62 white prepupae 12hr    58 white prepupae 24hr    96 pupae 2d postWPP    74 pupae 3d postWPP    33 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day    38 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr  (105) embryo 04-06hr  (164) embryo 06-08hr  (165) embryo 08-10hr  (173) embryo 10-12hr  (113) embryo 12-14hr  (133) embryo 14-16hr  (77) embryo 16-18hr  (59) embryo 18-20hr  (32) embryo 20-22hr  (44) embryo 22-24hr  (48) larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6  (28) larva L3 puffstage 7-9  (49) white prepupae new  (62) white prepupae 12hr  (58) white prepupae 24hr  (96) pupae 2d postWPP  (74) pupae 3d postWPP  (33) pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day  (38) adult female 30day  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr  105 embryo 04-06hr  (164) embryo 06-08hr  (165) embryo 08-10hr  (173) embryo 10-12hr  (113) embryo 12-14hr  (133) embryo 14-16hr    77 embryo 16-18hr    59 embryo 18-20hr    32 embryo 20-22hr    44 embryo 22-24hr    48 larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    49 white prepupae new    62 white prepupae 12hr    58 white prepupae 24hr    96 pupae 2d postWPP    74 pupae 3d postWPP    33 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day    38 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    105 embryo 04-06hr    164 embryo 06-08hr    165 embryo 08-10hr    173 embryo 10-12hr    113 embryo 12-14hr    133 embryo 14-16hr    77 embryo 16-18hr    59 embryo 18-20hr    32 embryo 20-22hr    44 embryo 22-24hr    48 larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    49 white prepupae new    62 white prepupae 12hr    58 white prepupae 24hr    96 pupae 2d postWPP    74 pupae 3d postWPP    33 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day    38 adult female 30day    34 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    105 embryo 04-06hr    164 embryo 06-08hr    165 embryo 08-10hr    173 embryo 10-12hr    113 embryo 12-14hr    133 embryo 14-16hr    77 embryo 16-18hr    59 embryo 18-20hr    32 embryo 20-22hr    44 embryo 22-24hr    48 larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    49 white prepupae new    62 white prepupae 12hr    58 white prepupae 24hr    96 pupae 2d postWPP    74 pupae 3d postWPP    33 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day    38 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr  (105) embryo 04-06hr  (164) embryo 06-08hr  (165) embryo 08-10hr  (173) embryo 10-12hr  (113) embryo 12-14hr  (133) embryo 14-16hr  (77) embryo 16-18hr  (59) embryo 18-20hr  32 embryo 20-22hr  (44) embryo 22-24hr  (48) larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6  28 larva L3 puffstage 7-9  (49) white prepupae new  (62) white prepupae 12hr  (58) white prepupae 24hr  (96) pupae 2d postWPP  (74) pupae 3d postWPP  (33) pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day  (38) adult female 30day  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr  105 embryo 04-06hr  (164) embryo 06-08hr  (165) embryo 08-10hr  (173) embryo 10-12hr  113 embryo 12-14hr  133 embryo 14-16hr    77 embryo 16-18hr    59 embryo 18-20hr    32 embryo 20-22hr    44 embryo 22-24hr    48 larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    49 white prepupae new    62 white prepupae 12hr    58 white prepupae 24hr    96 pupae 2d postWPP    74 pupae 3d postWPP    33 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day    38 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    105 embryo 04-06hr    164 embryo 06-08hr    165 embryo 08-10hr    173 embryo 10-12hr    113 embryo 12-14hr    133 embryo 14-16hr    77 embryo 16-18hr    59 embryo 18-20hr    32 embryo 20-22hr    44 embryo 22-24hr    48 larva L1    18 larva L2    11 larva L3 12hr old    5 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    49 white prepupae new    62 white prepupae 12hr    58 white prepupae 24hr    96 pupae 2d postWPP    74 pupae 3d postWPP    33 pupae 4d postWPP    8 adult male 01day    6 adult male 05day    4 adult male 30day    4 adult female 01day    23 adult female 05day    38 adult female 30day    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    56 embryonic Kc167    11 embryonic S1    47 embryonic S3    51 leg disc CME L1    28 wing disc CME-W2    58 wing disc ML-DmD8    97 wing disc ML-DmD9    53 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    41 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    73 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    284 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    56 ovary fGS/OSS    295 ovary OSC    87 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2  (56) embryonic Kc167    11 embryonic S1  (47) embryonic S3  (51) leg disc CME L1  (28) wing disc CME-W2  (58) wing disc ML-DmD8  (97) wing disc ML-DmD9  (53) wing disc ML-DmD16-c3  (39) wing disc ML-DmD21  (41) wing disc ML-DmD32  (40) haltere disc ML-DmD17-c3  (73) eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1  (284) CNS ML-DmBG2-c2  (65) tumorous blood cells mbn2  (56) ovary fGS/OSS  (295) ovary OSC  (87) ovary OSS  (85) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    56 embryonic Kc167    11 embryonic S1    47 embryonic S3    51 leg disc CME L1    28 wing disc CME-W2    58 wing disc ML-DmD8    97 wing disc ML-DmD9    53 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    41 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    73 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1  (284) CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    56 ovary fGS/OSS  (295) ovary OSC    87 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    56 embryonic Kc167    11 embryonic S1    47 embryonic S3    51 leg disc CME L1    28 wing disc CME-W2    58 wing disc ML-DmD8    97 wing disc ML-DmD9    53 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    41 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    73 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    284 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    56 ovary fGS/OSS    295 ovary OSC    87 ovary OSS    85 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    56 embryonic Kc167    11 embryonic S1    47 embryonic S3    51 leg disc CME L1    28 wing disc CME-W2    58 wing disc ML-DmD8    97 wing disc ML-DmD9    53 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    41 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    73 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    284 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    56 ovary fGS/OSS    295 ovary OSC    87 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2  (56) embryonic Kc167    11 embryonic S1  (47) embryonic S3  (51) leg disc CME L1  28 wing disc CME-W2  (58) wing disc ML-DmD8  (97) wing disc ML-DmD9  (53) wing disc ML-DmD16-c3  (39) wing disc ML-DmD21  (41) wing disc ML-DmD32  (40) haltere disc ML-DmD17-c3  (73) eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1  (284) CNS ML-DmBG2-c2  (65) tumorous blood cells mbn2  (56) ovary fGS/OSS  (295) ovary OSC  (87) ovary OSS  (85) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    56 embryonic Kc167    11 embryonic S1    47 embryonic S3    51 leg disc CME L1    28 wing disc CME-W2    58 wing disc ML-DmD8    97 wing disc ML-DmD9    53 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    41 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    73 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1  (284) CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    56 ovary fGS/OSS  (295) ovary OSC    87 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    23 embryonic 1182-4H    23 embryonic GM2    56 embryonic Kc167    11 embryonic S1    47 embryonic S3    51 leg disc CME L1    28 wing disc CME-W2    58 wing disc ML-DmD8    97 wing disc ML-DmD9    53 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    41 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    73 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    284 CNS ML-DmBG2-c2    65 tumorous blood cells mbn2    56 ovary fGS/OSS    295 ovary OSC    87 ovary OSS    85 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3  (35) Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult  (37) Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3  (36) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (32) Paraquat 10 mM 48 hrs, 4-day adult  (30) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3  (35) Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult  (37) Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3  (36) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  32 Paraquat 10 mM 48 hrs, 4-day adult  30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    16 cold shock, 4-day adult    15 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    35 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    36 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • LD09561 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011260 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4700-RC FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Sema-2a Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive Sema-2a03021 Sema-2a03474 Sema-2ak11240 viable Sema-2aCA06989 Sema-2aEY08184 viable, with Scer\GAL4pnr-MD237 Sema-2aGD5476 Sterility Allele fertile Sema-2aEY08184 Other Phenotypes Allele behavior defective | recessive Sema-2a03021 Sema-2a03474 flightless | recessive Sema-2a03021 Sema-2a03474 neuroanatomy defective, with Scer\GAL4elav-C155 Sema-2aEY08184 neuroanatomy defective, with Scer\GAL4how-24B Sema-2aScer\UAS.cKa neuroanatomy defective | embryonic stage, with Scer\GAL4tey-5053A Sema-2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a Phenotype manifest in Allele abdominal 1 VL3/4 motor neuron Sema-2ahs.Tl abdominal 1 VO2 motor neuron Sema-2ahs.Tl abdominal 2 VL3/4 motor neuron Sema-2ahs.Tl abdominal 2 VO2 motor neuron Sema-2ahs.Tl abdominal lateral oblique muscle 1 & neuromuscular junction Sema-2a03021 Sema-2a03474 Sema-2aex59 abdominal segmental nerve Sema-2a03021 Sema-2a03474 Sema-2aex59 abdominal segment border muscle & neuromuscular junction Sema-2a03021 Sema-2a03474 Sema-2aex59 abdominal ventral longitudinal muscle 2 & neuromuscular junction Sema-2a03021 Sema-2a03474 Sema-2aex59 abdominal ventral longitudinal muscle 3 & neuromuscular junction Sema-2a03021 Sema-2a03474 Sema-2aex59 abdominal ventral longitudinal muscle 4 & neuromuscular junction Sema-2a03021 Sema-2a03474 Sema-2aex59 imaginal disc Sema-2ak11240 intersegmental nerve Sema-2a03021 Sema-2a03474 Sema-2aex59 intersegmental nerve branch ISNb of A1-7, with Scer\GAL4tey-5053A Sema-2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a neuromuscular junction | ectopic Sema-2a03021 Sema-2a03474 Sema-2aex59 RP3 motor neuron Sema-2ahs.Tl RP3 motor neuron, with Scer\GAL4how-24B Sema-2aScer\UAS.cKa synapse, with Scer\GAL4elav-C155 Sema-2aEY08184 transverse nerve Sema-2a03021 Sema-2a03474 Sema-2aex59 transverse nerve, with Scer\GAL4A51 Sema-2aScer\UAS.cKa transverse nerve, with Scer\GAL4how-24B Sema-2aScer\UAS.cKa
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of Sema-2a Class Mutagen Stocks Known lesion Sema-2ak11240 P-element activity 2 -- Sema-2a03021 amorphic allele - genetic evidence, loss of function allele P-element activity Delta2-3 1 Yes Sema-2aCB-0871-3 1 -- Sema-2ae04687 piggyBac transposase 1 -- Sema-2aEY08184 P-element activity 1 -- Sema-2af06535 piggyBac transposase 1 -- Sema-2aKG03423 1 -- Sema-2aMI01715 1 -- Sema-2aMI03303 1 -- Sema-2aMI05602 1 -- Sema-2a03474 loss of function allele P-element activity Delta2-3 0 Yes Sema-2aCA06989 P-element activity 0 Yes Sema-2aCPTI000798 piggyBac transposase 0 Yes Sema-2aCPTI001481 piggyBac transposase 0 Yes Sema-2aCPTI001506 piggyBac transposase 0 Yes Sema-2aex59 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Sema-2a Class Mutagen Stocks Known lesion Sema-2aGD5476 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Sema-2aGL00356 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sema-2aHM05196 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sema-2a+tWa in vitro construct - genomic fragment 0 Yes Sema-2ahs.Tl in vitro construct - regulatory fusion 0 Yes Sema-2aNIG.4700R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Sema-2aScer\UAS.cKa in vitro construct - regulatory fusion 0 -- Sema-2aScer\UAS.T:Avic\GFP,T:Mmus\Cd8a in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Sema-2aSema-2b.L in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2R)Sema-2b-C4 (Wu et al., 2011)
Disrupted in	Df(2R)A15 (Wu et al., 2011) Df(2R)B65 (Wu et al., 2011)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5476} NA P{NIG.4700R} NA P{TRiP.GL00356} NA P{TRiP.HM05196} NA P{UAS-Sema-2a-TM-GFP} NA P{UAS-sema-II.K} NA heat-shock construct P{pCaSpeR-Toll-semaII} NA characterization construct PBac{Sema-2a.W} NA PBac{Sema-2b-L-Sema-2a} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Sema-2ak11240 No P{PZ}Sema-2a03021 No P{PZ}Sema-2a03474 No insertion of mobile activating element P{EPgy2}Sema-2aEY08184 NA miscellaneous insertions Mi{MIC}Sema-2aMI01715 NA Mi{MIC}Sema-2aMI03303 NA Mi{MIC}Sema-2aMI05602 NA P{PTT-GA}Sema-2aCA06989 NA P{RS3}Sema-2aCB-0871-3 NA P{SUPor-P}Sema-2aKG03423 NA PBac{602.P.SVS-1}Sema-2aCPTI000798 NA PBac{681.P.FSVS-1}Sema-2aCPTI001481 NA PBac{681.P.FSVS-1}Sema-2aCPTI001506 NA PBac{RB}Sema-2ae04687 NA PBac{WH}Sema-2af06535 NA
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with UniProtKB:Q9V4A7 (assigned by UniProtKB) (Ayoob et al., 2006)
Biological Process
CV term Evidence References
adult behavior inferred from mutant phenotype (Kolodkin et al., 1993) axon guidance inferred from mutant phenotype (Matthes et al., 1995) dendrite guidance inferred from genetic interaction with Sema-2b (Sweeney et al., 2011) drinking behavior inferred from mutant phenotype (Kolodkin et al., 1993) flight behavior inferred from mutant phenotype (Kolodkin et al., 1993) salivary gland development inferred from mutant phenotype (Vining et al., 2002) synaptic target inhibition inferred from mutant phenotype (Chiba, 1999, Wu et al., 2011) visual behavior inferred from mutant phenotype (Kolodkin et al., 1993)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
receptor activity inferred from electronic annotation with InterPro:IPR016201 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
adult behavior non-traceable author statement (Swiss-Prot Project Members, 2000.2.15) axon guidance non-traceable author statement (Swiss-Prot Project Members, 2000.2.15) synaptic target inhibition non-traceable author statement (Swiss-Prot Project Members, 2000.2.15)
Cellular Component
CV term Evidence References
extracellular region inferred from sequence or structural similarity (Kolodkin et al., 1993) non-traceable author statement (Senti et al., 1999, Swiss-Prot Project Members, 2000.2.15) membrane inferred from electronic annotation with InterPro:IPR016201 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Sema-2a - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - CG10916 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - CG5323 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - Crc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - Pdi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - Pngl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - Ten-m anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Sema-2a - plexB genetic interference, affinity technology, light microscopy, tag visualisation (Wu et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Sema-2a allele Gene References Sema-2a03021 Kr (Abrell et al., 2000) Sema-2a03474 Fas2 (Winberg et al., 1998) Sema-2aScer\UAS.cKa Fas2 (Winberg et al., 1998)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62559 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011260 DroID - A comprehensive database of gene and protein interactions. • FBgn0011260 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36846
Orthologs
OrthoDB Orthologs (32) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6KPTW8 Dipteran inclusive ortholog search EOG6M920J Insect inclusive ortholog search EOG6QRG01 Arthropod inclusive ortholog search EOG6CJSZ4 Metazoa inclusive ortholog search EOG66MQBF
Orthologs in Drosophila Species (EOG6KPTW8)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Sema-2a     Drosophila simulans   Dsim\GD11202 Y   Drosophila sechellia   Dsec\GM21705 Y   Drosophila erecta   Dere\GG20610 Y   Drosophila yakuba   Dyak\GE11800 Y   Drosophila ananassae   Dana\GF13604 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA24470 Y   Drosophila persimilis   Dper\GL10746 Y   Drosophila willistoni   Dwil\GK21702 Y   Drosophila virilis   Dvir\GJ22377 Y   Drosophila mojavensis   Dmoj\GI20528 Y   Drosophila grimshawi   Dgri\GH21241 Y
Orthologs in non-Drosophila Dipterans (EOG6M920J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005993   Anopheles gambiae Malaria mosquito  Agam\AGAP006636   Anopheles gambiae Malaria mosquito  Agam\AGAP006634   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017234
Orthologs in non-Dipteran Insects (EOG6QRG01)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB46091 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG017731 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22320 Y Atta cephalotes Leafcutter ant  Acep\ACEP24363 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17804 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19212 Y Linepithema humile Argentine ant  Lhum\LH15542 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB25478 Y Solenopsis invicta Red fire ant  Sinv\SINV14366 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008055 Y Bombyx mori Silkmoth  Bmor\BGIBMGA008511 Y Pediculus humanus Human body louse  Phum\PHUM307950 Y Tribolium castaneum Red flour beetle  Tcas\Sema-2a Y
Orthologs in non-Insect Arthropods (EOG6CJSZ4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Hoxb8 Y Ixodes scapularis Deer tick  Isca\ISCW016640 Y
Orthologs in non-Arthropod Metazoa (EOG66MQBF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\mab-20 Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11202 Drosophila sechellia Dsec\GM21705 Drosophila erecta Dere\GG20610 Drosophila yakuba Dyak\GE11800 Drosophila ananassae Dana\GF13604 Drosophila pseudoobscura pseudoobscura Dpse\GA24470 Drosophila persimilis Dper\GL10746 Drosophila willistoni Dwil\GK21702 Drosophila virilis Dvir\GJ22377 Drosophila mojavensis Dmoj\GI20528 Drosophila grimshawi Dgri\GH21241
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	29519 y1 v1; P{TRiP.HM05196}attP2 33135 y1 w*; Mi{MIC}Sema-2aMI01715 37785 y1 w*; Mi{MIC}Sema-2aMI03303 43016 y1 w*; Mi{MIC}Sema-2aMI05602 35432 y1 sc* v1; P{TRiP.GL00356}attP2 11257 cn1 P{PZ}Sema-2a03021/CyO; ry506 17437 y1 w67c23; P{EPgy2}Sema-2aEY08184
Harvard	- PBac{RB}Sema-2ae04687 - PBac{WH}Sema-2af06535
Kyoto	123351 w1118; P{RS3}Sema-2aCB-0871-3
VDRC	v15810 w1118; P{GD5476}v15810/TM3 v15811 w1118; P{GD5476}v15811/CyO
	More stocks available...
Genomic Clones ( 1 )
	BACR06I15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 131 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD09306 LD09561 RE45189
Other clones	DPiM_LD09306 RT01502
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD03628 LD03680 LD04426 LD05691 LD07023 LD10146 LD11216 LD12113 LD18563 LD47367 LP14695 RE01847 RE04948 RE08850 RE13672 RE15156 RE15286 RE17432 RE22663 RE23105 RE27678 RE29556 RE30360 RE31948 RE33933 RE36360 RE37565 RE38145 RE39077 RE41396 RE46478 RE53521 RE62042 RE65929 RE66433 RE67015 RE67476 RE67554 RE69894 RE71222 RE71577 RE74424 RE75142 RH02684 RH42952
Other clones	153702 277131 381330 CK01.25A.G12 CK01.104C.E2 CK02190 Dmel_dig_010724_0658_2551 EK027722 EK030421 EK052406 EK054102 EK059534 EK076946 EK087649 EK094826 EK112657 EK169530 EK172835 EK172844 EK187522 EK193009 EK194521 EK198947 EK216408 EK220135 EK246206 EK255644 EK257733 EK258424 EK264705 EK299658 EK304414 FGM306D05 FGM324D11 RP003096906 RP003179237 RP003199574 RP003246481 RP003292272 RP003295269 RP003472577 RP003500085 RP003530063 RP003587676 RP004048698 RP004077836 RP004090166 RP004092247 RP004134549 RP004165867 RP004173508 RP004179705 RP004181561 RP004192891 RP004204602 RP004204702 RP004213797 RP004215888 RP004216860 RP004232851 RP004258281 RP004261914 RP004352801 RP004370439 RP004379850 RP004407608 RP004430818 RP004446692 RP004448599 RP004452207 RP004462597 RP004489175 RP004490965 RP004506886 RP004507370 RP004513391 RP004530179 RP004530802 RP004533993 RP004540672 RP004549110
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011260 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36846
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Sema-2a BcDNA:LD09561 (FlyBase, 1996-)
Additional comments	Source for merge of Sema-2a BcDNA:LD09561 was a shared cDNA (date:030206). (FlyBase, 1996-)
Other Comments
	Sema-2a and Sema-2b provide spatial cues for projection neuron dendrite targeting. (Sweeney et al., 2011) Sema-2a and Sema-2b are both ligands of the plexB receptor, but they have different guidance functions during neurogenesis. Sema-2b functions at short-range as a guidance cue to promote axonal attraction, mediating axon-axon recognition and fasciculation, whereas Sema-2a acts over a broader range as a repulsive cue, to prevent aberrant innervation. (Wu et al., 2011) Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology. (Roch et al., 1998) Sema-2a prevents promiscuous and ectopic synapse formation. Relative balance of Sema-2a and Fas2 controls synaptogenesis. The relative balance of Sema-2a and the Netrins controls the choice of a specific target and the avoidance of a potential target. NetB and Sema-2a can both be repulsive for certain motor axons. (Winberg et al., 1998) Sema-2a can function as a inhibitory signal during target recognition, it inhibits two identified motoneuron growth cones RP3 and DC1 from forming normal synaptic terminal aborizations on their target muscles. RP1 and RP4 are unresponsive to contact with Sema-2a. (Matthes et al., 1995) Genetic analysis reveals that semaII is an essential gene required for both proper adult behaviour and survival. (Kolodkin et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058159 NM_134303 NM_166178 RefSeq (Proteins) • NP_477507 NP_599130 NP_725586 DDBJ / EMBL / GenBank • AA264846 AA390412 AAC37187 AAF57989 AAF57990 AAM11354 AAM68500 AAM75030 AAS64837 AC004432 AC005147 AC005647 AQ073276 AW942069 AW942079 AY095026 AY128437 BI358423 CL525990 CZ482468 CZ489243 CZ489244 L26083 Entrez Gene - A searchable database of RefSeq genes. • 36846 UniProtKB/Swiss-Prot • Q24323
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WD40/YVTN repeat-like-containing domain (IPR015943) Semaphorin/CD100 antigen (IPR001627) Immunoglobulin subtype (IPR003599) Immunoglobulin-like domain (IPR007110) Plexin/semaphorin/integrin (IPR003659) Plexin-like fold (IPR016201) Semaphorin (IPR027231) Immunoglobulin-like fold (IPR013783) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD09561
Linkouts
	BioGRID - A database of protein and genetic interactions • 62559 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0011260 DroID - A comprehensive database of gene and protein interactions. • FBgn0011260 DRSC - Results from RNAi screens. • FBgn0011260 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011260 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4700-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011260 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36846 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/semapr21.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36846 modMine - Data generated by the modENCODE project. • FBgn0011260
Synonyms & Secondary IDs ( 27 )
Reported As
Symbol Synonym	112/40 (Roch et al., 1998) BcDNA:LD09561   CG4700 (Wu et al., 2011, Perkins et al., 2009, Ni et al., 2011.7.19) CT14922 (Hynes and Zhao, 2000) D-Sema2a (Vining et al., 2002) Dsema-II (Kolodkin, 1994.2.3) D-SemaII (Pini, 1994, Keynes and Cook, 1995) dSema-II (Henderson et al., 1996) D-semaII (Kolodkin et al., 1993) D-Sema II (Seeger, 1994) D-Sema-II   l(2)03021 (Abrell et al., 2000) Sema2a (Senti et al., 1999, Chiba and Rose, 1998, Winberg et al., 1998, Tessier-Lavigne and Goodman, 1996, Bate and Broadie, 1995, BDGP Project Members, 1994-1999, Kolodkin et al., 1993, Girard et al., 2006) Sema-2a (Buszczak et al., 2007, Wu et al., 2011, Carrillo et al., 2010, Wu et al., 2011, Franciscovich et al., 2008, Ayoob et al., 2006, Japanese National Institute of Genetics, 2012.5.21) sema-2a (Sweeney et al., 2011, Kucherenko et al., 2008) sema2a (Ismat et al., 2008, Bates and Whitington, 2007) sema 2a (Zlatic et al., 2009) sema-II (Spradling et al., 1999, ) sema II (Englund et al., 1999) SemaII (Andrew, 1998, Winberg et al., 1998, Andrew, 1998, Winberg et al., 1997, Garrity and Zipursky, 1995) semaII (Dodd and Schuchardt, 1995, Matthes et al., 1995)
Name Synonym	D-Semaphorin II (Goodman, 1995) Sema-2a (Cho et al., 2005) semaphorin (Dudu et al., 2004, Hughes and Salinas, 1999) semaphorin 2a (Ayoob et al., 2006) Semaphorin II (Anonymous, 1998) semaphorin II   semiphorin (Ackley and Jin, 2004)
Secondary FlyBase IDs
	FBgn0062466
References ( 77 )
Generate a list of	All references relating to this gene ( 77 )
List References by type	Research paper  ( 24 ) Supplementary material  ( 6 ) Review  ( 13 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 9 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 ) Letter  ( 1 )
Recent research papers ( 3 )
	Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Sweeney et al., 2011, Neuron 72(5): 734--747 Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting. [FBrf0216932] Wu et al., 2011, Neuron 70(2): 281--298 A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. [FBrf0213571] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...