NF-κB
Please see the JBrowse view of Dmel\Dif for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.50
2.8 (northern blot)
667 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dif using the Feature Mapper tool.
Comment: 12 hr APF
Dif protein is expressed in the larval fat body.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Dif in GBrowse 22-53
2-52.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: Dif CG6794
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Tl pathway is required for the nuclear import of dl in the immune response, but not required for the nuclear import of Dif. Cytoplasmic retention of both dl and Dif depends on cact protein. The two signalling pathways that target cact for degradation must discriminate between cact-dl and cact-Dif complexes.
The molecular evolution of the Rel/NF-κB and IκB proteins is studied in parallel. Phylogenetic analysis allows the structure of the putative ancestor genes to be defined and proposes and evolutionary model that clusters both families in a unique Rel/NF-κB/IκB superfamily.
Antibacterial genes are not induced in L.boulardi resistant strains of Drosophila suggesting they cannot be involved directly in the antiparasitoid response.
Dif is a rel family protein that is thought to function in the humoral immune response.
The Dif gene product is a sequence specific transcription factor which trans-activates the CecA1 gene in cotransfection assays. Transactivation requires a 40bp upstream element including an insect κΒ-like motif. dl can also activate the CecA1 promoter, but to a lesser extent and in a less sequence-specific manner than Dif. The dl product exerts a dominant negative effect on Dif transactivation of CecA1.