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Gene Dmel\lbe

General Information
Symbol	Dmel\lbe	Species	D. melanogaster
Name	ladybird early	Annotation symbol	CG6545
Feature type	protein_coding_gene	FlyBase ID	FBgn0011278
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	lb
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	93E1-93E1	Sequence location	3R:17,267,299..17,272,259 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene ladybird early is referred to in FlyBase by the symbol Dmel\lbe (CG6545, FBgn0011278). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity. There is experimental evidence for 14 unique biological process terms, many of which group under: single-organism developmental process; biological regulation; system development; post-embryonic organ morphogenesis; regulation of developmental process; neuron differentiation; regulation of RNA metabolic process; cellular component organization or biogenesis; localization; myoblast proliferation; muscle cell differentiation. 9 alleles are reported. The phenotypes of these alleles are annotated with: abdominal adult muscle precursor cell; multicellular structure; organ system; anatomical structure; organ system subdivision; ISN innervated muscle of A1-7; hypodermal muscle of larval abdomen; peripheral nervous system; embryonic/larval hypodermal muscle; embryonic/larval neuron. It has one annotated transcript and one annotated polypeptide. Protein features are: Helix-turn-helix motif; Homeobox, conserved site; Homeodomain; Homeodomain, metazoa; Homeodomain-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed within 06-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1634056_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of lbe. Gene sequence location is 3R:17267299..17272259.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93E1-93E1   Limits computationally determined from genome sequence between P{PZ}mod(mdg4)07038&P{lacW}mod(mdg4)L3101 and P{PZ}InR05545
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93E1-93E1     (Jagla, 1996.10.14) 93D9-93E2     (Jagla, 1993.12.16) 93D9-93E2   (determined by in situ hybridisation)   (Ranz et al., 1997) 93D-93E   (determined by in situ hybridisation)   (Jagla et al., 1994)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\lbe for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.40 Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) lbe-RA FBtr0084091  NM_079711   2277   479
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein lbe-PA   FBpp0083490   52.2   479   8.50     NP_524435   AAF55896
Additional Polypeptide Data & Comments
Reported size (kDa)	479 (aa) (Jagla et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Homeobox, conserved site (IPR017970) Helix-turn-helix motif (IPR000047) Homeodomain (IPR001356) Homeodomain-like (IPR009057) Homeodomain, metazoa (IPR020479)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF55896   BT126373   L08618 AAA28612   Y08821 CAA70056   Z29082 CAA82323
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KS77 Q9VDA1 Q7JMT6 Q61676
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage | late   presumptive embryonic/larval peripheral nervous system | restricted   (Jagla et al., 1997) central nervous system | restricted   (Jagla et al., 1997) segment border muscle   (Jagla et al., 1997) embryonic stage 10 -- 11   embryonic neuroblast   (Jagla et al., 1997) embryonic stage 10 -- 13   dorsal ectoderm of abdominal segment 1 -- abdominal segment 7   (Jagla et al., 1997) embryonic stage 11   epidermis | segmentally repeated   (Jagla et al., 1994) embryonic stage 13   ectoderm | ventral   (Jagla et al., 1997) embryonic stage 15   epidermis | dorsal   (Jagla et al., 1997) Comment:early embryonic stage 15
Additional Descriptive Data
	lbe transcript appears earlier, and is more abundant, than the lbl transcript. lbe transcript first appears at stage 8 of embryogenesis, in the anal plate primordium, and subsequently in stage 10-11, in some neuroblasts and in the ectoderm of gnathal segments. At stage 10-11, expression is also detected in a cluster of cells corresponding to the heart primordium. During stage 10-13, lbe and lbl are both expressed in the dorsal ectoderm of abdominal segments 1-7, and to a lesser extent, in the ventral ectoderm. At stage 15, lbl and FBgn0011278:lbe transcripts persist in the dorsal, but not in the ventral epidermis. Later in embryogenesis, FBgn0008651:lbl and FBgn0011278:lbe@ transcripts are restricted to clusters of cells in the central and peripheral nervous systems, and to the segment border muscles. (Jagla et al., 1997) Using RT-PCR, lbe transcript is detected early in embryogenesis, about 2 hours before lbl, and persists through embryogenesis. Using in-situ hybridization, lbe transcript is detected in stage 11 embryos, in the dorsal and ventral epidermis in a segmentally repeated pattern. lbl and lbe are expressed in cells just anterior to those expressing en. (Jagla et al., 1994)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunohistochemistry Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   dorsal head epidermis   (Zikova et al., 2003) embryonic stage 14   embryonic/larval heart anchoring cell   (Zikova et al., 2003) embryonic stage 14 -- 16   frontal sac   (Zikova et al., 2003) Comment:tip of the frontal sac immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage | late   presumptive embryonic/larval peripheral nervous system | restricted   (Jagla et al., 1997) segment border muscle   (Jagla et al., 1997) central nervous system | restricted   (Jagla et al., 1997) embryonic stage 9 -- 11   procephalic neurogenic region | restricted   (Urbach and Technau, 2003) protocerebral neuroblast | subset   (Urbach and Technau, 2003) tritocerebral neuroblast | subset   (Urbach and Technau, 2003) deuterocerebral neuroblast | subset   (Urbach and Technau, 2003) embryonic stage 9 -- 17   anal pad primordium   (Jagla et al., 1997) embryonic stage 10 -- 11   ectoderm of embryonic gnathal segment   (Jagla et al., 1997) embryonic stage 10 -- 13   dorsal ectoderm of abdominal segment 1 -- abdominal segment 7   (Jagla et al., 1997) embryonic stage 13   ectoderm | ventral   (Jagla et al., 1997) embryonic stage 14   cardioblast | segmentally repeated & subset   (Perrin et al., 2004) embryonic stage 14 -- 15   segment border muscle | precursor   (Jagla et al., 1999) embryonic stage 15   epidermis | dorsal   (Jagla et al., 1997) Comment:early stage 15
Additional Descriptive Data
	lbe gene product appears earlier, and is more abundant, than the lbl gene product. lbe protein first appears at stage 8 of embryogenesis, in the anal plate primordium, and subsequently in stage 10-11, in some neuroblasts and in the ectoderm of gnathal segments. At stage 10-11, expression is also detected in a cluster of cells corresponding to the heart primordium. During stage 10-13, lbe and lbl are both expressed in the dorsal ectoderm of abdominal segments 1-7, and to a lesser extent, in the ventral ectoderm. At stage 15, lb and lbe gene products persist in the dorsal, but not in the ventral epidermis. Later in embryogenesis, lbl and lbe gene products are restricted to clusters of cells in the central and peripheral nervous systems, and to the segment border muscles. (Jagla et al., 1997) Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d4; deuterocerebrum - d7; protocerebrum - cv8, pv3 (Urbach and Technau, 2003) lbe is expressed in the two central cardioblasts of every hemisegment. (Perrin et al., 2004)
Marker for
	cardioblast
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Jagla et al., 1997, Han et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.1 Larval Midgut    2 Larval Hindgut    7.1 Larval Malpighian Tubules    2.2 Larval Fat Body    11.4 Larval Salivary Gland    19.8 Larval Trachea    4.85 Larval Carcass    4.375 Adult Head    5 Adult Eye    2.15 Adult Brain    2.9 Adult Thoracic-Abdominal Ganglion    2.1 Adult Crop    5.4 Adult Midgut    8.7 Adult Hindgut    2.7 Adult Malpighian Tubules    4.9 Adult Fat Body    3.8 Adult Salivary Gland    19 Adult Heart    1.775 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    5 Adult Ovary    0.5 Adult Testis    10.1 Adult Male Accessory Gland    5.4 Adult Carcass    7.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    5   NA  Eye    2.15   NA  Brain    2.9   3.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.1   NA  Crop    5.4   2  Midgut    8.7   7.1  Hindgut    2.7   2.2  Malpighian Tubules    4.9   no informative data  Fat Body    3.8   no informative data  Salivary Gland    no informative data   NA  Heart    1.775   4.85  Trachea    NA   NA  VirginFemale Spermatheca    4.9   NA  InseminatedFemale Spermatheca    5   NA  Ovary    0.5   NA  Testis    no informative data   NA  Male Accessory Gland    5.4   4.375  Carcass    7.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed within 06-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    1 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_21 mE1_20_mRNA_expression_cluster_08
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011278 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6545-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Mef2.PR lbeGD4157 viable, with Scer\GAL4pnr-MD237 lbeGD4157 Other Phenotypes Allele locomotor behavior defective, with Scer\GAL41151 lbeScer\UAS.cJa Phenotype manifest in Allele A1-7 dorsal acute muscle 1, with Scer\GAL4how-24B lbeScer\UAS.cJa A1-7 dorsal oblique muscle 1, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 1 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 1 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 2 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 2 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 3 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 3 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 4 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 4 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 5 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 5 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 6 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 6 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 7 lateral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa abdominal 7 ventral adult muscle precursor cell, with Scer\GAL4how-24B lbeScer\UAS.cJa aorta primordium, with Scer\GAL4how-24B lbeScer\UAS.cJa cardiac outflow muscle, with Scer\GAL4how-24B lbeScer\UAS.cJa central nervous system, with Scer\GAL4l(3)31-1-31-1 lbeScer\UAS.cJa dorsal denticle lbehs.PJ EL neuron, with Scer\GAL4l(3)31-1-31-1 lbeScer\UAS.cJa embryonic/larval dorsal vessel | precursor lbehs.PJ embryonic/larval somatic muscle | lateral, with Scer\GAL4how-24B lbeScer\UAS.cJa exit glial cell | ectopic, with Scer\GAL4l(3)31-1-31-1 lbeScer\UAS.cJa glial cell | ectopic, with Scer\GAL4l(3)31-1-31-1 lbeScer\UAS.cJa intersegmental nerve, with Scer\GAL4l(3)31-1-31-1 lbeScer\UAS.cJa muscle fiber, with Scer\GAL41151 lbeScer\UAS.cJa myoblast, with Scer\GAL41151 lbeScer\UAS.cJa prothoracic segmental nerve, with Scer\GAL4l(3)31-1-31-1 lbeScer\UAS.cJa segment border muscle, with Scer\GAL4how-24B lbeScer\UAS.cJa tarsal levator muscle, with Scer\GAL41151 lbeScer\UAS.cJa
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of lbe Class Mutagen Stocks Known lesion lbeDf311 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of lbe Class Mutagen Stocks Known lesion lbeGD4157 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes lbeJF03011 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes lbeKK109261 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes lbedsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes lbehs.PJ in vitro construct - regulatory fusion 0 Yes lbeNIG.6545R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes lbeorg-1.HN39.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes lbeScer\UAS.cJa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3R)e-F1 (De Graeve et al., 2004) Df(3R)lb (De Graeve et al., 2004)
Disrupted in	Df(3R)e-BS2 (Jagla et al., 1997)
Not disrupted in	Df(3R)e-F1 (Jagla et al., 1997) Df(3R)e-Gp4 (Jagla et al., 1997) Df(3R)GC14 (Jagla et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{lbe-lacZ.LAMPE.ETS.mut} No P{lbe-lacZ.LAMPE.lb.mut} No P{lbe-lacZ.LAMPE} No P{lbe-lacZ.LME.ETS.mut} No P{lbe-lacZ.LME.Mef2.mut} No P{lbe-lacZ.LME} No UAS construct P{GD4157} NA P{KK109261} NA P{NIG.6545R} NA P{TRiP.JF03011} NA P{UAS-lbe.J} NA GAL4 construct P{lbe-GAL4.K} No heat-shock construct P{hs-lbe} NA characterization construct P{org1-HN39-lbe} NA P{UAS-lbe.RNAi} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 21 unique terms )
Terms Based on Experimental Evidence ( 16 terms )
Molecular Function
CV term Evidence References
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity inferred from direct assay (Han et al., 2002)
Biological Process
CV term Evidence References
cell migration inferred from genetic interaction with lbl (Junion et al., 2007) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) epidermis development inferred from mutant phenotype (Jagla et al., 1997) genital disc morphogenesis inferred from mutant phenotype (Jagla et al., 1997) imaginal disc-derived leg morphogenesis inferred from genetic interaction with lbl (Maqbool et al., 2006) muscle organ development inferred from mutant phenotype (Parrish et al., 2006) myoblast fusion inferred from genetic interaction with lbl (Junion et al., 2007) myoblast proliferation inferred from genetic interaction with lbl (Maqbool et al., 2006) negative regulation of transcription from RNA polymerase II promoter inferred from expression pattern (Han et al., 2002) neuron development inferred from mutant phenotype (Parrish et al., 2006) regulation of cell fate specification inferred from mutant phenotype (Jagla et al., 2002) regulation of neuron differentiation inferred from mutant phenotype (De Graeve et al., 2004) regulation of transcription, DNA-dependent inferred from mutant phenotype (Jagla et al., 1997) skeletal muscle tissue development inferred from genetic interaction with lbl (Junion et al., 2007)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Jagla et al., 1997, Han et al., 2002)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with InterPro:IPR001356 AND non-traceable author statement (FlyBase, 1992-) transcription regulatory region sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR000047 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
central nervous system development non-traceable author statement (Jagla et al., 2001) heart development traceable author statement (Jagla et al., 2001) mesodermal cell fate specification traceable author statement (Jagla et al., 2001) muscle organ development traceable author statement (Jagla et al., 2001) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with InterPro:IPR001356 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with InterPro:IPR001356 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions lbe allele Gene References lbehs.PJ lbl (Jagla et al., 2002)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0011278 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42542
Orthologs
OrthoDB Orthologs (7) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6GJ093 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6GJ093)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\lbe     Drosophila simulans   Dsim\GD19976 Y   Drosophila sechellia   Dsec\GM11006 Y   Drosophila erecta   Dere\GG14495 Y   Drosophila yakuba   Dyak\GE24975 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19675 Y   Drosophila persimilis   Dper\GL24419 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19976 Drosophila sechellia Dsec\GM11006 Drosophila erecta Dere\GG14495 Drosophila yakuba Dyak\GE24975 Drosophila ananassae Dana\GF16415 Drosophila pseudoobscura pseudoobscura Dpse\GA19675 Drosophila persimilis Dper\GL24419 Drosophila willistoni Dwil\GK11027 Drosophila virilis Dvir\GJ14417 Drosophila mojavensis Dmoj\GI10818 Drosophila grimshawi Dgri\GH18718
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	28374 y1 v1; P{TRiP.JF03011}attP2
VDRC	v102377 P{KK109261}VIE-260B v12662 w1118; P{GD4157}v12662
Genomic Clones ( 1 )
	BACR34E13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 1 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	MIP30310
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0011278 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42542
Antibody Information
	monoclonal (Maqbool et al., 2006, Perrin et al., 2004) polyclonal (Jagla et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: lbe Hox11-D125 (FlyBase, 1996-)
Additional comments	Source for merge of lbe Hox11-D125 was sequence comparison (date:030604). (FlyBase, 1996-)
Other Comments
	The attenuation of lbe and lbl may affect muscle fibre assembly and contractility, leading to reduced leg muscle performance. (Maqbool et al., 2006) dsRNA has been made from templates generated with primers directed against this gene. RNAi of lbe results in increased arborization of ddaD and ddaE neurons. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development. (Parrish et al., 2006) Cross-repressive interactions between the "identity genes" lbe, lbl, Dr and eve are essential for the specification of cardiac and muscular fates in the developing embryo. (Jagla et al., 2002) The specific epidermal expression patterns of lbe and lbl and their regulatory interactions with wg are analysed. Activity of lbe and lbl in the epidermis is regulated by the segment polarity gene network and depends on wg signalling. lbe and lbl are required in the dorsal epidermis and for anal plate development. (Jagla et al., 1997) lbe and lbl are involved in the specification of a segmental subset of heart precursors during the early phase of cardiogenesis, and are required for maintaining wg activity during the late phase of cardiogenesis. (Jagla et al., 1997) Cloning and characterisation of homeobox genes lbe and lbl. (Jagla et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079711 RefSeq (Proteins) • NP_524435 DDBJ / EMBL / GenBank • AAA28612 AAF55896 CAA70056 CAA82323 L08618 Y08821 Z29082 Entrez Gene - A searchable database of RefSeq genes. • 42542 UniProtKB/TrEMBL • Q7JMT6 Q7KS77 Q9VDA1 Q61676
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Homeobox, conserved site (IPR017970) Helix-turn-helix motif (IPR000047) Homeodomain (IPR001356) Homeodomain-like (IPR009057) Homeodomain, metazoa (IPR020479)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0011278 DRSC - Results from RNAi screens. • FBgn0011278 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011278 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6545-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011278 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42542 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/ladybrd1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42542 modMine - Data generated by the modENCODE project. • FBgn0011278
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG6545 (Keleman et al., 2009.8.5, Perkins et al., 2009) Hox11-D125   lb (Zikova et al., 2003, De Graeve et al., 2004, Reim et al., 2003, Cripps and Olson, 2002, Knirr et al., 1999, Frasch, 1999, Paululat et al., 1999, Ruiz-Gomez, 1998, Junion et al., 2007, Klingseisen et al., 2009, Figeac et al., 2010) Lb (Lo and Frasch, 2003, Zaffran and Frasch, 2000, Bataillé et al., 2007, Monier et al., 2007, Schaub et al., 2012, Carrasco-Rando et al., 2011, Figeac et al., 2010) Lbe (Perrin et al., 2004, Jagla, 1993.12.16, Rogulja-Ortmann et al., 2007, Parrish et al., 2006, Zaffran et al., 2006, Albrecht et al., 2006, Sellin et al., 2009, Figeac et al., 2010) lbe (Urbach et al., 2006, Liu et al., 2006, Hoskins et al., 2005, Maqbool et al., 2006, Dietzl et al., 2007, Philippakis et al., 2006, Balakirev et al., 2011, Sandmann et al., 2006, Liu et al., 2008, Parrish et al., 2007, Liu et al., 2009, Junion et al., 2007, Buechling et al., 2009, Copley, 2005, Klingseisen et al., 2009, Zeitlinger et al., 2007, Zeitlinger et al., 2007, Jagla et al., 1997, Bantignies et al., 2011, Baumgardt et al., 2009, Zmojdzian et al., 2008, Karlsson et al., 2010, Figeac et al., 2010, Jagla et al., 1999, Müller et al., 2010, Cherbas et al., 2011, Tolhuis et al., 2011, Karim and Moore, 2011, Schönbauer et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Cheutin and Cavalli, 2012) NK?
Name Synonym	ladybird (Clark et al., 2005, Urbach and Technau, 2002, Bidet et al., 2001, Hughes and Salinas, 1999, Nguyen and Xu, 1998, Baylies et al., 1998, Tajbakhsh and Sporle, 1998, Ruiz Gomez and Bate, 1997, Bataillé et al., 2007, Albrecht et al., 2006, Junion et al., 2007, Zeitlinger et al., 2007, Figeac et al., 2010) Ladybird (Lo and Frasch, 2003, Carrasco-Rando et al., 2011) ladybird early (Jagla et al., 1997, Maqbool et al., 2006, Philippakis et al., 2006, Liu et al., 2008, Junion et al., 2007, Buechling et al., 2009, Baumgardt et al., 2009, Figeac et al., 2010, Jagla et al., 1999, Beckervordersandforth et al., 2008, Schönbauer et al., 2011) lady-bird-early (Jagla et al., 1994)
Secondary FlyBase IDs
	FBgn0010394
References ( 111 )
Generate a list of	All references relating to this gene ( 111 )
List References by type	Research paper  ( 70 ) Supplementary material  ( 5 ) Review  ( 13 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 11 ) FlyBase analysis  ( 2 ) Conference report  ( 1 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 10 )
	Cheutin and Cavalli, 2012, PLoS Genet. 8(1): e1002465 Progressive polycomb assembly on H3K27me3 compartments generates polycomb bodies with developmentally regulated motion. [FBrf0218702] Schaub et al., 2012, Development 139(5): 1001--1012 Org-1, the Drosophila ortholog of Tbx1, is a direct activator of known identity genes during muscle specification. [FBrf0217455] Balakirev et al., 2011, PLoS ONE 6(7): e22613 Complex Interplay of Evolutionary Forces in the ladybird Homeobox Genes of Drosophila melanogaster. [FBrf0214564] Bantignies et al., 2011, Cell 144(2): 214--226 Polycomb-Dependent Regulatory Contacts between Distant Hox Loci in Drosophila. [FBrf0212775] Carrasco-Rando et al., 2011, PLoS Genet. 7(7): e1002186 Drosophila araucan and caupolican integrate intrinsic and signalling inputs for the acquisition by muscle progenitors of the lateral transverse fate. [FBrf0214635] Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Karim and Moore, 2011, J. Neurosci. 31(47): 17017--17027 Convergent local identity and topographic projection of sensory neurons. [FBrf0216770] Schönbauer et al., 2011, Nature 479(7373): 406--409 Spalt mediates an evolutionarily conserved switch to fibrillar muscle fate in insects. [FBrf0216735] Tolhuis et al., 2011, PLoS Genet. 7(3): e1001343 Interactions among Polycomb Domains Are Guided by Chromosome Architecture. [FBrf0213356] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...