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General Information
Symbol
Dmel\lbe
Species
D. melanogaster
Name
ladybird early
Annotation Symbol
CG6545
Feature Type
FlyBase ID
FBgn0011278
Gene Model Status
Stock Availability
Gene Snapshot
ladybird early (lbe) encodes a transcription factor involved in muscle and heart development. It also plays a role in regulating the embryonic expression of wg. [Date last reviewed: 2019-09-12]
Also Known As
ladybird, lb
Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:21,441,577..21,446,537 [-]
Recombination map
3-72
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
NK-LIKE HOMEOBOX TRANSCRIPTION FACTORS -
NK-like (NKL) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. NKL transcription factors are homeobox genes closely related to Hox-like genes, a number of which are found in the NK cluster. Many of the NKL members contain an Engrailed Homology 1 (EH1) motif. (Adapted from FBrf0232555 and PMID:22094586).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\lbe or the JBrowse view of Dmel\lbe for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.40
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084091
2277
479
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0083490
52.2
479
8.50
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lbe using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (20 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002388800
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR017970
(assigned by InterPro )
Biological Process (17 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002388800
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
epidermis | dorsal

Comment: early embryonic stage 15

Additional Descriptive Data
lbe transcript appears earlier, and is more abundant, than the lbl transcript. lbe transcript first appears at stage 8 of embryogenesis, in the anal plate primordium, and subsequently in stage 10-11, in some neuroblasts and in the ectoderm of gnathal segments. At stage 10-11, expression is also detected in a cluster of cells corresponding to the heart primordium. During stage 10-13, lbe and lbl are both expressed in the dorsal ectoderm of abdominal segments 1-7, and to a lesser extent, in the ventral ectoderm. At stage 15, lbl and FBgn0011278:lbe transcripts persist in the dorsal, but not in the ventral epidermis. Later in embryogenesis, FBgn0008651:lbl and FBgn0011278:lbe @ transcripts are restricted to clusters of cells in the central and peripheral nervous systems, and to the segment border muscles.
Using RT-PCR, lbe transcript is detected early in embryogenesis, about 2 hours before lbl, and persists through embryogenesis. Using in-situ hybridization, lbe transcript is detected in stage 11 embryos, in the dorsal and ventral epidermis in a segmentally repeated pattern. lbl and lbe are expressed in cells just anterior to those expressing en.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunohistochemistry
Stage
Tissue/Position (including subcellular localization)
Reference
frontal sac

Comment: tip of the frontal sac

immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
In stage 14 embryos, embryonic heart anchoring cells (HANCs) expressing lbe reach the tin-positive cardioblasts at the level of thoracic segment T2.
abdominal lateral adult muscle precursor cell ; FBbt:00003243
lbe is dynamically expressed in myoblasts associated with the leg imaginal disc proper. All leg myoblasts express twi. lbe is expressed initially in early third instar larvae in a small subset of twi-expressing cells located in the dorsal femur and giving rise to the levator muscle. Most ventrally, lbe marks individual myoblasts that give rise to the tibial depressor muscle. The number of leg myoblasts expressing lbe gradually increases, and at the beginning of pupation, all of them express lbe at least at a low level. Myoblasts associated with the proximal portion of the leg disc are lbe-negative. 3D analysis reveals that lbe myoblasts form spatially restricted groups of cells lying at highly stereotyped positions that can be correlated with defined muscles in the adult leg. In the tibia and femur at 0hr APF, the groups of lbe-expressing myblasts are located dorsally and ventrally at positions representing the levator and the depressor muscles. They are also seen at muscle-forming sites for the trochanter and coxa. lbe is also expressed in leg disc epithelium in the ventral region and in the long tendon.
lbe is expressed in the two central cardioblasts of every hemisegment.
Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d4; deuterocerebrum - d7; protocerebrum - cv8, pv3
lbe gene product appears earlier, and is more abundant, than the lbl gene product. lbe protein first appears at stage 8 of embryogenesis, in the anal plate primordium, and subsequently in stage 10-11, in some neuroblasts and in the ectoderm of gnathal segments. At stage 10-11, expression is also detected in a cluster of cells corresponding to the heart primordium. During stage 10-13, lbe and lbl are both expressed in the dorsal ectoderm of abdominal segments 1-7, and to a lesser extent, in the ventral ectoderm. At stage 15, lb and lbe gene products persist in the dorsal, but not in the ventral epidermis. Later in embryogenesis, lbl and lbe gene products are restricted to clusters of cells in the central and peripheral nervous systems, and to the segment border muscles.
Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\lbe in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of lbe
Transgenic constructs containing regulatory region of lbe
Deletions and Duplications ( 6 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (20)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
6 of 15
No
Yes
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (14)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
5 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (14)
7 of 13
Yes
Yes
3 of 13
No
Yes
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (12)
2 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (17)
8 of 15
Yes
No
7 of 15
No
No
6 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (10)
2 of 9
Yes
Yes
2 of 9
Yes
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190BX4 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila erecta
Drosophila yakuba
Drosophila yakuba
Drosophila yakuba
Drosophila ananassae
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila virilis
Drosophila mojavensis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150F0F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0JVJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0K1C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G09CY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (11)
6 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-72
    Cytogenetic map
    Sequence location
    3R:21,441,577..21,446,537 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    93E1-93E1
    Limits computationally determined from genome sequence between P{PZ}mod(mdg4)07038&P{lacW}mod(mdg4)L3101 and P{PZ}InR05545
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    93D9-93E2
    (determined by in situ hybridisation)
    93D-93E
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (25)
    cDNA Clones (1)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of
          Source for merge of: lbe Hox11-D125
          Additional comments
          Source for merge of lbe Hox11-D125 was sequence comparison ( date:030604 ).
          Other Comments
          The attenuation of lbe and lbl may affect muscle fibre assembly and contractility, leading to reduced leg muscle performance.
          dsRNA has been made from templates generated with primers directed against this gene. RNAi of lbe results in increased arborization of ddaD and ddaE neurons. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
          Cross-repressive interactions between the "identity genes" lbe, lbl, Dr and eve are essential for the specification of cardiac and muscular fates in the developing embryo.
          The specific epidermal expression patterns of lbe and lbl and their regulatory interactions with wg are analysed. Activity of lbe and lbl in the epidermis is regulated by the segment polarity gene network and depends on wg signalling. lbe and lbl are required in the dorsal epidermis and for anal plate development.
          lbe and lbl are involved in the specification of a segmental subset of heart precursors during the early phase of cardiogenesis, and are required for maintaining wg activity during the late phase of cardiogenesis.
          Cloning and characterisation of homeobox genes lbe and lbl.
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 35 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (13)
          Reported As
          Symbol Synonym
          Hox11-D125
          NK?
          Secondary FlyBase IDs
          • FBgn0010394
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (157)