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General Information
Symbol
Dmel\RyR
Species
D. melanogaster
Name
Ryanodine receptor
Annotation Symbol
CG10844
Feature Type
FlyBase ID
FBgn0011286
Gene Model Status
Stock Availability
Gene Snapshot
Ryanodine receptor (RyR) encodes an intracellular calcium channel localized in the sarcoplasmic reticulum of muscles and the endoplasmic reticulum of neurons and other cell types. It regulates the release of intracellular calcium stores and has a key role in muscle contraction. [Date last reviewed: 2018-09-13]
Also Known As

Rya-r44F, Ryanodine receptor 44F, l(2)k04913, dry, DRR

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:8,860,364..8,888,097 [+]
Recombination map

2-59

RefSeq locus
NT_033778 REGION:8860364..8888097
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the ryanodine receptor (TC 1.A.3.1) family. (Q24498)
Summaries
Gene Group (FlyBase)
OTHER CALCIUM CHANNEL-FORMING SUBUNITS -
The other calcium channel-forming subunits are a collection of proteins forming homomultimeric channels that do not fall into the main classes of calcium channels. (Adapted from PMID:12838335).
Protein Function (UniProtKB)
Intracellular calcium channel that is required for proper muscle function during embryonic development and may be essential for excitation-contraction coupling in larval body wall muscles.
(UniProt, Q24498)
Summary (Interactive Fly)

Ca2+-induced Ca2+ release channel located in presynaptic endoplasmic reticulum membranes - sustains vesicular mobilization thus enhancing release of transmitters and neuropeptides

Gene Model and Products
Number of Transcripts
10
Number of Unique Polypeptides
10

Please see the GBrowse view of Dmel\RyR or the JBrowse view of Dmel\RyR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088640
15812
5127
FBtr0088638
15770
5113
FBtr0088637
15847
5127
FBtr0088639
15770
5113
FBtr0304568
16003
5117
FBtr0304569
15788
5119
FBtr0304570
16056
5123
FBtr0304571
16042
5130
FBtr0304572
15871
5134
FBtr0308083
15811
5115
Additional Transcript Data and Comments
Reported size (kB)

16 (northern blot)

15 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087721
581.0
5127
5.32
FBpp0087719
579.7
5113
5.32
FBpp0087718
580.8
5127
5.29
FBpp0087720
579.5
5113
5.29
FBpp0293110
580.0
5117
5.29
FBpp0293111
580.3
5119
5.32
FBpp0293112
580.5
5123
5.33
FBpp0293113
581.3
5130
5.37
FBpp0293114
581.4
5134
5.32
FBpp0300426
579.9
5115
5.40
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homotetramer.

(UniProt, Q24498)
Domain

The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.

(UniProt, Q24498)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RyR using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (17 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001416417
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
traceable author statement
(assigned by UniProt )
Biological Process (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
traceable author statement
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN001416417
(assigned by GO_Central )
Cellular Component (8 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346459
(assigned by GO_Central )
non-traceable author statement
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN001416417
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000346461
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 6-12 hr AEL

Additional Descriptive Data

RyR transcript is first detected in the mesoderm at stage 9, and levels increase starting at stage 13. Highest levels are seen in hypodermal and visceral muscles. Expression is detected at lower levels in other tissues, including the CNS.

Expression is high in 6-12 hr embryos, low in second and third instar larvae, and absent from adults and pupae.

RyR transcripts are detected in RNA from embryos, larvae, and adults, but not in pupal RNA. They are more abundant in RNA from body than head. In embryos, transcripts are first detected in the mesoderm in stage 9. Subsequently, transcripts are found in somatic and cephalic muscle precursors, and later, in all of the somatic muscles and a subset of the cephalic muscles. In adults, RyR transcripts are expressed maximally in tubular muscles of the thorax, namely the tergotrochanteral muscle and the leg muscles. Expression is also observed in the cortex of the ventral ganglion and brain and in muscles of the head and proboscis.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RyR in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 21 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RyR
Transgenic constructs containing regulatory region of RyR
Deletions and Duplications ( 12 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
14 of 15
Yes
Yes
12 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
13 of 15
Yes
Yes
11 of 15
No
Yes
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
11 of 13
Yes
Yes
10 of 13
No
Yes
8 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (5)
9 of 12
Yes
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (10)
11 of 15
Yes
Yes
9 of 15
No
Yes
9 of 15
No
Yes
6 of 15
No
Yes
6 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919000H )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915000B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0009 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0008 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00T0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (1)
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Homotetramer.
(UniProt, Q24498 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map

2-59

Cytogenetic map
Sequence location
2R:8,860,364..8,888,097 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
44F1-44F2
Limits computationally determined from genome sequence between P{PZ}l(2)44DEa05847 and P{lacW}Rya-r44Fk04913
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
44F1-44F2
(determined by in situ hybridisation)
44F-44F
(determined by in situ hybridisation)
Stated (in error) to map to 76C--D (by in situ hybridisation).
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (24)
Genomic Clones (26)
cDNA Clones (78)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: RyR Rya-r44F

    Source for database merge of

    Source for merge of: Rya-r44F CG10844

    Source for merge of: Rya-r44F l(2)k04913

    Additional comments

    FlyBase curator comment: Gene symbol changed from 'Rya-r44F' to 'RyR' because: i) it represents community usage; ii) it mirrors the nomenclature of the vertebrate orthologs; iii) there is only a single RyR-encoding gene in the genome, so there's no need to distinguish this one.

    Other Comments

    Removing one copy of RyR protects against Hsap\APP neurotoxicity.

    Area matching Drosophila Ryanodine receptor gene Acc. No. D17389.

    Rya-r44F is required for proper muscle function.

    Isolated from a genomic library using a rabbit skeletal muscle ryanodine receptor cDNA as a probe.

    The genomic organisation of the Rya-r44F locus has been characterised.

    The Drosophila ryanodine receptor gene was cloned by PCR with degenerate oligonucleotides.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 91 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (26)
    Reported As
    Symbol Synonym
    Rya-r76CD
    dya
    Secondary FlyBase IDs
    • FBgn0010268
    • FBgn0022194
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (139)