General Information
Symbol
Dmel\Snap25
Species
D. melanogaster
Name
Synaptosomal-associated protein 25kDa
Annotation Symbol
CG40452
Feature Type
FlyBase ID
FBgn0011288
Gene Model Status
Stock Availability
Gene Snapshot
Synaptosomal-associated protein 25kDa (Snap25) is a synaptic target SNARE protein and a component of SNARE protein complexes known to function in neurotransmitter release. Snap25 functions in synaptic transmission in larvae and is essential in adults. [Date last reviewed: 2016-09-01]
Also Known As
SNAP-25
Genomic Location
Cytogenetic map
Sequence location
3L:24,074,180..24,299,205 [+]
Recombination map
3-47
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the SNAP-25 family. (P36975)
Molecular Function (see GO section for details)
Summaries
Gene Group Membership
Qbc-SNAREs -
Q-SNAREs are members of the SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein (SNAP) receptor) family that mediate vesicle-fusion. SNAREs are cytoplasmic, membrane-anchored proteins that contain a 60 amino acid SNARE domain. Three Q-SNAREs domains from the target membrane and one R-SNARE domain from the vesicle interact to form a four four-helix bundle that pulls the two membranes in close proximity and promotes fusion. After fusion, the complexes are dissembled by NSF (N-ethylmaleimide-sensitive factor) and SNAPs (Soluble NSF Attachment Proteins). Q-SNAREs are named from their contribution of a conserved glutamine (Q) residue to the helix bundle. Q-SNARE domains are designated a, b or c, depending on their position within the helix bundle. Qbc-SNAREs (or SNAPs (synaptosomal-associated proteins) possess a Qb and Qc domain, thus contribute two helices to the bundle. (Adapted from PMID:15893389 and FBrf0216182).
UniProt Contributed Function Data
May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion (By similarity).
(UniProt, P36975)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Snap25 or the JBrowse view of Dmel\Snap25 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.50
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0111143
2233
212
FBtr0304625
2233
212
Additional Transcript Data and Comments
Reported size (kB)
2.3 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0110435
23.7
212
4.24
FBpp0293167
23.7
212
4.20
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
25 (kD observed)
212 (aa); 23.7 (kD predicted)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Snap25 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (21 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000447753
(assigned by GO_Central )
inferred from sequence or structural similarity
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000447753
(assigned by GO_Central )
Biological Process (11 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000447753
(assigned by GO_Central )
inferred from sequence or structural similarity
non-traceable author statement
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000447754
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000447754
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000447753
(assigned by GO_Central )
non-traceable author statement
Cellular Component (7 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
colocalizes_with BLOC-1 complex
inferred from sequence or structural similarity with UniProtKB:P60880
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:Q17QQ3
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:P60879
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:P60879
(assigned by UniProt )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000447752
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000447753
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:P60880-1
(assigned by UniProt )
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Snap25 protein is detected at high levels starting at embryonic stage 14. Most intense staining is seen in the dorsal extent of the ventral nerve cord, which is rich in synapses, and the esophageal ganglion. At stage 16, chordotonal organs of the PNS also stain for Snap25 protein.
Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Snap25 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 29 )
Transgenic Constructs ( 13 )
Deletions and Duplications ( 6 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
No
8 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
No
8 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
6 of 13
Yes
No
3 of 13
No
Yes
1 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
10 of 12
Yes
No
5 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (6)
12 of 15
Yes
No
12 of 15
Yes
Yes
12 of 15
Yes
Yes
7 of 15
No
Yes
6 of 15
No
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
14 of 15
Yes
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (3)
7 of 9
Yes
Yes
6 of 9
No
Yes
6 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
2 of 15
Yes
No
2 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
4 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190F9Y )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila erecta
Drosophila yakuba
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150ATM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CCG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CAH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0N7J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-47
    Cytogenetic map
    Sequence location
    3L:24,074,180..24,299,205 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    FlyBase curator comment: FBrf0067338 stated that Snap25 mapped to clone DS04327 and thus cytological location 64E11--65B5, however subsequent analysis by Drosophila Heterochromatin Genome Project authoritatively determined that Snap25 lies in 3L heterochromatin.
    FlyBase curator comment: FBrf0127207 stated that Snap25 mapped to 64E--65B, determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones, however subsequent analysis by Drosophila Heterochromatin Genome Project authoritatively determined that Snap25 lies in 3L heterochromatin.
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (58)
    cDNA Clones (202)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Source for merge of: Snap25 CG17884 CG17676
    Source for merge of: CG17676 CG17884
    Additional comments
    Annotations CG17676 and CG17884 merged as CG40452 in release 3 of the genome annotation.
    Other Comments
    Snap25 has an evolutionarily conserved and complex gene organisation. The onset of Snap25 expression correlates with a time in neuronal development when synapses begin to be formed and when other synapse-specific genes are switched on.
    Isolated from a Drosophila head cDNA library using a chicken SNAP-25 cDNA fragment (encoding the 5' half of the coding region) as a probe, under low stringency conditions.
    Snap25 has been cloned and sequenced, and its RNA expression pattern has been analysed.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 40 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    InterPro - A database of protein families, domains and functional sites
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (13)
    Reported As
    Symbol Synonym
    CG17676
    CG17884
    dSNAP-25
    Name Synonyms
    Synaptosomal-associated protein 25kDa
    synaptosomal-associated protein 25
    Secondary FlyBase IDs
    • FBan0040452
    • FBgn0039960
    • FBgn0039996
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (107)