FlyBase Gene Report: Dmel\a5

General Information

Symbol

Dmel\a5

Species

D. melanogaster

Name

antennal protein 5

Annotation Symbol

CG5430

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011294

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Snapshot

Insufficient genetic data for FlyBase to solicit a summary. [Date last reviewed: 2016-06-30]

Other Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

22A1-22A1

Sequence location

2L:1,329,161..1,329,993 [+]

Recombination map

2-3

RefSeq locus

NT_033779 REGION:1329161..1329993

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Protein Family (UniProt)

Belongs to the phosphatidylethanolamine-binding protein family. (P54185)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

Predictions / Assertions

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\a5 or the JBrowse view of Dmel\a5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

a5-RA

FBtr0077922

NM_057650

776

210

Additional Transcript Data and Comments

Reported size (kB)

0.687 (longest cDNA)

(Pikielny et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

a5-PA

FBpp0077588

24.6

210

4.53

NP_476998

AAF51385

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

210 (aa)

(Pikielny et al., 1994)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P54185

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\a5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

a5_1

Linkouts

Gene Ontology (0 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

third segment of antenna

(Pikielny et al., 1994)

sensillum coeloconicum of antennal segment 3

(Pikielny et al., 1994)

Additional Descriptive Data

a5 transcripts are detected in RNA made from appendages but not head or body. They are restricted to a few scattered sensilla on the posterior surface of the antenna that are probably coeloconic sensilla.

(Pikielny et al., 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\a5 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\a5

View exonic expression by tissue for Dmel\a5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0011294

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5430-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0011294

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of a5

Transgenic constructs containing regulatory region of a5

Deletions and Duplications ( 1 )

Disrupted in

Df(2L)Exel8004

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

a5^GD14736

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

a5^GD14736

viable, with Scer\GAL4^tin.CΔ4

a5^GD14736

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

a5^GD14736

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PEBP1

6 of 15

Yes

Hsap\PEBP4

5 of 15

Hsap\MRPL38

2 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Pbp2

6 of 15

Yes

Mmus\Pebp4

5 of 15

Mmus\Pebp1

4 of 15

Mmus\Mrpl38

2 of 15

Rattus norvegicus (Norway rat) (4)

Rnor\Pbp2

5 of 13

Yes

Rnor\Pebp1

5 of 13

Yes

Rnor\Mrpl38

2 of 13

Rnor\Pebp4

2 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\pebp1

5 of 12

Yes

Xtro\pebp4

4 of 12

Xtro\LOC100497751

2 of 12

Xtro\mrpl38

2 of 12

Danio rerio (Zebrafish) (3)

Drer\pebp1

5 of 15

Yes

Drer\pebp4

3 of 15

Drer\mrpl38

2 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\Y69E1A.5

6 of 15

Yes

Cele\F40A3.3

5 of 15

Cele\C56G2.4

1 of 15

Yes

Cele\mrpl-38

1 of 15

Arabidopsis thaliana (thale-cress) (7)

Atha\E12A11

7 of 9

Yes

Atha\FT

7 of 9

Yes

Atha\TSF

7 of 9

Yes

Atha\BFT

6 of 9

Atha\TFL1

6 of 9

Yes

Atha\ATC

5 of 9

Atha\AT5G01300

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\TFS1

6 of 15

Yes

Scer\YLR179C

6 of 15

Yes

Scer\MRPL35

5 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC2F12.10

4 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190F3Y )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18875

Drosophila willistoni

Dwil\GK26794

Drosophila virilis

Dvir\GJ17014

Drosophila mojavensis

Dmoj\GI14554

Drosophila grimshawi

Dgri\GH11408

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AI3 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17012896

Glossina morsitans

Tsetse fly

Gmor\GMOY005343

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07349

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0KH6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_013584gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_001716gn

Gallus gallus

Domestic chicken

Ggal\PEBP1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

5 of 10

4 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PEBP1; phosphatidylethanolamine binding protein 1

6 of 15

604591

PEBP4; phosphatidylethanolamine binding protein 4

5 of 15

612473

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59566

DroID - A comprehensive database of gene and protein interactions.

FBgn0011294

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33317

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-3

Cytogenetic map

22A1-22A1

Sequence location

2L:1,329,161..1,329,993 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

22A1-22A1

Limits computationally determined from genome sequence between P{PZ}l(2)10685¹⁰⁶⁸⁵&P{lacW}Tango14^k00619 and P{lacW}RFeSP^k11704

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

22A-22B

(Pikielny, 1994.1.18)

Experimentally Determined Recombination Data

Location

2-3

(FlyBase, 2016)

2-2.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Bloomington

55932

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04220}attP40

VDRC

v100657

P{KK106233}VIE-260B

v44713

w¹¹¹⁸ P{GD14736}v44713

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011294

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011294

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33317

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: a5 CG5430

(Li, 1999.11)

Source for database merge of

Additional comments

Other Comments

a5 is one of seven clones that have been identified from subtracted cDNA libraries, containing antennal cDNA from which head cDNAs have been subtracted. The protein is expressed in subsets of olfactory hairs and have a putative signal peptide at the amino terminus. a5 protein is highly similar both to vertebrate brain proteins that bind hydrophobic ligands and to yeast and nematode proteins.

(Pikielny et al., 1994)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 27 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33317

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information